Moutan Cortex terpene glycoside is derived from the dried root bark of Paeonia suffruticosa Andr.in the Paeoniaceae family,which holds significant value as a traditional Chinese medicine.This study investigated that M...Moutan Cortex terpene glycoside is derived from the dried root bark of Paeonia suffruticosa Andr.in the Paeoniaceae family,which holds significant value as a traditional Chinese medicine.This study investigated that Moutan Cortex terpene glycoside(MCTG)improved diabetic kidney disease(DKD)by targeting sirtuin 1(SIRT1)mediated autophagy pathway.Mechanistic insights were gained using DKD model rats and human umbilical vein endothelial cells(HUVECs)to delineate how MCTG operated in the treatment of DKD.Furthermore,network pharmacology was used to identify the primary metabolic pathways affected by MCTG,with key targets being confirmed through polymerase chain reaction(PCR),Western blot,Transmission electron microscope,immunofluorescence staining and monodansylcadaverine(MDC)staining.Finally,small interfering RNA transfection testified SIRT1 in advanced glycation end-products(AGEs)-induced HUVECs injury.MCTG effectively decreased blood glucose rise in DKD rats and reduced levels of cytokines and biochemical indicators.Network pharmacology revealed that metabolism was the main pathway of Moutan Cortex,and the main targets were verified by PCR and protein experiments.Based on these results,we found that Moutan Cortex could improve DKD and SIRT1 was a potential target.Furthermore,knockdown of SIRT1 attenuated AGEs-induced the expression of Beclin 1 and microtubule-associated protein 1 light chain 3 II/I(LC3 II/I)in HUVECs.In summary,this study demonstrated that Moutan Cortex could alleviate DKD via down-regulating SIRT1-mediated autophagy pathway.展开更多
To the Editor:Abeleda et al.[1]recently argued in a perspective article that the Philippines should do better in order to achieve its goal of reducing schistosomiasis incidence to zero in humans,animals,and snails by ...To the Editor:Abeleda et al.[1]recently argued in a perspective article that the Philippines should do better in order to achieve its goal of reducing schistosomiasis incidence to zero in humans,animals,and snails by 2025[2,3].The authors appropriately ascribed the need to ramp up surveillance,WASH(water,sanitation,and hygiene),and veterinary efforts.In this letter,I would like to add to the discussion that the key to achieving schistosomiasis elimination may be strengthening the veterinary public health sector and framing mitigation efforts within the One Health lens.Schistosoma(S.)japonicum infection is a zoonotic disease and it affects more than 40 species of animals,including livestock animals like cattle,pigs,and water buffaloes,and pets like dogs[4].In the Philippines,the prevalence of infection in animal reservoirs remains largely unknown in most endemic areas due to the lack of concerted epidemiological efforts unlike what is being done in human hosts.In areas where studies have been done,infection rates are from less than 10%to 97%in water buffaloes tested[5-7].Indeed,as Abeleda et al.[1]argued,there is a need to do better on this front.展开更多
The circumscriptions of sections Auganthus Pax ex Balf. f. and Ranunculoides Chen et C. M. Hu in the genus Primula L. have been controversial, due to the different treatments of P. filchnerae Knuth. W...The circumscriptions of sections Auganthus Pax ex Balf. f. and Ranunculoides Chen et C. M. Hu in the genus Primula L. have been controversial, due to the different treatments of P. filchnerae Knuth. We conducted a phylogenetic analysis using sequences from the ITS region of nuclear ribosomal DNA. All five species of these two sections and some representatives of other related sections were sampled. The ITS trees show that P. filchnerae could be convincingly placed in sect. Auganthus , together with P. sinensis Sabine ex Lindl. and P. rupestris Balf. f. et Farrer, whereas sect. Ranunculoides contains two species: P. cicutariifolia Pax and P. merrilliana Schltr. These two sections are distantly related and their association with other sections needs further studies. This study also demonstrated that ITS sequences would be a suitable marker for the reconstruction of Primula phylogeny.展开更多
[Objective] To utilize the germplasm resources,new systematic evidence for the classification of sect.Paracamellia,genus Camellia is found in this article.[Method] Fruit anatomy of 10 species representing sect.Paracam...[Objective] To utilize the germplasm resources,new systematic evidence for the classification of sect.Paracamellia,genus Camellia is found in this article.[Method] Fruit anatomy of 10 species representing sect.Paracamellia was investigated.And the main component of sect.Paracamellia in the genus Camellia was studied by MVSP software.[Result] The exocarp was thin;mesocarp was broad,having a large number of stone cells;endocarp was lignified,and separated easily from seed.The principle components analysis suggested that the distance between species was far,showing far relation;the current study did not support the formation of Camellia grijsii（C.grijsii）and Camellia yuhsienensis（C.yuhsienensis）,Camellia shensiensis（C.shensiensis）should not be recognized as a variety of C.yuhsienensis.In addition,it also proved Ming＇s combining of Camellia confusa（C.confusa）and Camellia kissi（C.kissi）was unnatural.[Conclusion] The study provided theoretical basis for the development and application of sect.Paracamellia in the genus Camellia.展开更多
Yunnan is not only the center of tea origination but also the center of tea evolution and variation.There is a broad distribution of Sect.Thea（L.） Dyer in Yunnan.This paper concerns the research progress of Sect.The...Yunnan is not only the center of tea origination but also the center of tea evolution and variation.There is a broad distribution of Sect.Thea（L.） Dyer in Yunnan.This paper concerns the research progress of Sect.Thea（L.） Dyer,reveals the distribution of Sect.Thea（L.） Dyer in Yunnan,and checks out the number and name of Sect.Thea（L.） Dyer in Yunnan,which will offer references for further study and application of tea plants.展开更多
Editor's Note:As a unique system of succession in Tibetan Buddhism,the reincarnation of Living Buddhas is governed by standardized religious rituals,historical conventions,and established principles.Since the Karm...Editor's Note:As a unique system of succession in Tibetan Buddhism,the reincarnation of Living Buddhas is governed by standardized religious rituals,historical conventions,and established principles.Since the Karma Kagyu sect pioneered the reincarnation system of Living Buddhas in the 13th century,this tradition has continued for more than 700 years.Since the Gelug sect adopted the reincarnation practice in the 16th century,resulting in major lineages such as the Dalai Lama and Panchen Erdeni reincarnations,the system has now held a history of over 400 years.Since the Yuan Dynasty(1271-1368),successive central governments of China have,without exception,strengthened administration over reincarnation matters.In the 57th year of the reign of Emperor Qianlong(1792)of the Qing Dynasty,the Golden Urn Lot-Drawing System was formally established.This system has remained in place up to the present day.Demonstrating national sovereignty,governmental authority,as well as the sacredness and impartiality of Buddhism,it has effectively prevented corruption and malpractice during the reincarnation process.展开更多
电感储能型脉冲电源是目前电磁发射技术的研究热点,提升装置储能密度对于推动电感储能型脉冲电源的应用和研究具有重要意义。本文介绍了一种适用于电感储能型脉冲电源的名为meat grinder with SECT的新电路,并对该电路进行了详细的原理...电感储能型脉冲电源是目前电磁发射技术的研究热点,提升装置储能密度对于推动电感储能型脉冲电源的应用和研究具有重要意义。本文介绍了一种适用于电感储能型脉冲电源的名为meat grinder with SECT的新电路,并对该电路进行了详细的原理分析,指出该电路兼具电流关断能力强和拓扑结构简单的优点。通过仿真将meat grinder with SECT和STRETCH meat grinder、STRETCH meat grinder with ICCOS这两种主流电路进行了性能比较,发现meat grinder with SECT电路在保持电气性能不变的同时,成本最低,体积最小。基于meat grinder with SECT电路,本文研制了50k J电感储能型脉冲电源并进行了试验测试,测试结果达到了预期目标。展开更多
The present study was designed to investigate the therapeutic effcts of Moutan Cortex(CM,root bark of Paeonia suffruticosa Andr) and Paeoniae Radix Rubra(PR,root of Paeonia veitchii Lynch) on metabolic disorders,focus...The present study was designed to investigate the therapeutic effcts of Moutan Cortex(CM,root bark of Paeonia suffruticosa Andr) and Paeoniae Radix Rubra(PR,root of Paeonia veitchii Lynch) on metabolic disorders,focusing on the infuence of CM and PR on the obesity-related gut microbiota homeostasis.The diet-induced obese(DIO) mouse model was used to test the therapeutic effects of CM and PR.The mice were orally administered with CM and PR for 6 weeks,and oral glucose tolerance test(OGTT) and insulin tolerance test(ITT) were performed to evaluate the insulin sensitivity of the mice.Sterol-regulatory element binding proteins(SREBPs) and their target genes were measured by quantitative RT-PCR.High-throughput 16 S ribosomal RNA(16S rR NA) gene sequencing technology was used to determine the composition of gut microbiota,and the metabolites in serum were analyzed by GC-MS.Our results indicated that CM and PR combination alleviated obese and insulin resistance in the DIO mice,leading to increased glucose uptake and gene expression in muscle and liver,and down-regulated SREBPs and their target genes in liver.Interesting,neither the CM-PR extracts,nor the major components of CM and PR did not affect SREBPs activity in cultured cells.Meanwhile,CM and PR significantly modulated the gut microbiota of the high-fat diet(HFD) treated mice,similar to metformin,and CM-PR reversed the overall microbiota composition similar to the normal chow diet(NCD) treated mice.In conclusion,our results provide novel mechanisms of action for the effects of CM and PR in treating DIO-induced dysregulation of sugar and lipid metabolism.展开更多
基金supported by grants from the National Natural Science Foundation of China(82474093,81973536)Jiangsu Province“Blue and Green Project”(184080H10240)+2 种基金Graduate Research Innovation Program of Jiangsu(KYCX23_0871)the National Natural Science Foundation of the Youth Science Fund Project(81703775)Health Research Program of Wuxi Municipal Health Commission(Q202107).
文摘Moutan Cortex terpene glycoside is derived from the dried root bark of Paeonia suffruticosa Andr.in the Paeoniaceae family,which holds significant value as a traditional Chinese medicine.This study investigated that Moutan Cortex terpene glycoside(MCTG)improved diabetic kidney disease(DKD)by targeting sirtuin 1(SIRT1)mediated autophagy pathway.Mechanistic insights were gained using DKD model rats and human umbilical vein endothelial cells(HUVECs)to delineate how MCTG operated in the treatment of DKD.Furthermore,network pharmacology was used to identify the primary metabolic pathways affected by MCTG,with key targets being confirmed through polymerase chain reaction(PCR),Western blot,Transmission electron microscope,immunofluorescence staining and monodansylcadaverine(MDC)staining.Finally,small interfering RNA transfection testified SIRT1 in advanced glycation end-products(AGEs)-induced HUVECs injury.MCTG effectively decreased blood glucose rise in DKD rats and reduced levels of cytokines and biochemical indicators.Network pharmacology revealed that metabolism was the main pathway of Moutan Cortex,and the main targets were verified by PCR and protein experiments.Based on these results,we found that Moutan Cortex could improve DKD and SIRT1 was a potential target.Furthermore,knockdown of SIRT1 attenuated AGEs-induced the expression of Beclin 1 and microtubule-associated protein 1 light chain 3 II/I(LC3 II/I)in HUVECs.In summary,this study demonstrated that Moutan Cortex could alleviate DKD via down-regulating SIRT1-mediated autophagy pathway.
文摘To the Editor:Abeleda et al.[1]recently argued in a perspective article that the Philippines should do better in order to achieve its goal of reducing schistosomiasis incidence to zero in humans,animals,and snails by 2025[2,3].The authors appropriately ascribed the need to ramp up surveillance,WASH(water,sanitation,and hygiene),and veterinary efforts.In this letter,I would like to add to the discussion that the key to achieving schistosomiasis elimination may be strengthening the veterinary public health sector and framing mitigation efforts within the One Health lens.Schistosoma(S.)japonicum infection is a zoonotic disease and it affects more than 40 species of animals,including livestock animals like cattle,pigs,and water buffaloes,and pets like dogs[4].In the Philippines,the prevalence of infection in animal reservoirs remains largely unknown in most endemic areas due to the lack of concerted epidemiological efforts unlike what is being done in human hosts.In areas where studies have been done,infection rates are from less than 10%to 97%in water buffaloes tested[5-7].Indeed,as Abeleda et al.[1]argued,there is a need to do better on this front.
文摘The circumscriptions of sections Auganthus Pax ex Balf. f. and Ranunculoides Chen et C. M. Hu in the genus Primula L. have been controversial, due to the different treatments of P. filchnerae Knuth. We conducted a phylogenetic analysis using sequences from the ITS region of nuclear ribosomal DNA. All five species of these two sections and some representatives of other related sections were sampled. The ITS trees show that P. filchnerae could be convincingly placed in sect. Auganthus , together with P. sinensis Sabine ex Lindl. and P. rupestris Balf. f. et Farrer, whereas sect. Ranunculoides contains two species: P. cicutariifolia Pax and P. merrilliana Schltr. These two sections are distantly related and their association with other sections needs further studies. This study also demonstrated that ITS sequences would be a suitable marker for the reconstruction of Primula phylogeny.
基金Supported by General Program of Jinhua City Science and Technology Bureau(2009-2-020)~~
文摘[Objective] To utilize the germplasm resources,new systematic evidence for the classification of sect.Paracamellia,genus Camellia is found in this article.[Method] Fruit anatomy of 10 species representing sect.Paracamellia was investigated.And the main component of sect.Paracamellia in the genus Camellia was studied by MVSP software.[Result] The exocarp was thin;mesocarp was broad,having a large number of stone cells;endocarp was lignified,and separated easily from seed.The principle components analysis suggested that the distance between species was far,showing far relation;the current study did not support the formation of Camellia grijsii（C.grijsii）and Camellia yuhsienensis（C.yuhsienensis）,Camellia shensiensis（C.shensiensis）should not be recognized as a variety of C.yuhsienensis.In addition,it also proved Ming＇s combining of Camellia confusa（C.confusa）and Camellia kissi（C.kissi）was unnatural.[Conclusion] The study provided theoretical basis for the development and application of sect.Paracamellia in the genus Camellia.
基金Supported by Foundation of Tea Research Institute of Yunnan Acad-emy of Agricultural Science (2009A09-37)~~
文摘Yunnan is not only the center of tea origination but also the center of tea evolution and variation.There is a broad distribution of Sect.Thea（L.） Dyer in Yunnan.This paper concerns the research progress of Sect.Thea（L.） Dyer,reveals the distribution of Sect.Thea（L.） Dyer in Yunnan,and checks out the number and name of Sect.Thea（L.） Dyer in Yunnan,which will offer references for further study and application of tea plants.
文摘Editor's Note:As a unique system of succession in Tibetan Buddhism,the reincarnation of Living Buddhas is governed by standardized religious rituals,historical conventions,and established principles.Since the Karma Kagyu sect pioneered the reincarnation system of Living Buddhas in the 13th century,this tradition has continued for more than 700 years.Since the Gelug sect adopted the reincarnation practice in the 16th century,resulting in major lineages such as the Dalai Lama and Panchen Erdeni reincarnations,the system has now held a history of over 400 years.Since the Yuan Dynasty(1271-1368),successive central governments of China have,without exception,strengthened administration over reincarnation matters.In the 57th year of the reign of Emperor Qianlong(1792)of the Qing Dynasty,the Golden Urn Lot-Drawing System was formally established.This system has remained in place up to the present day.Demonstrating national sovereignty,governmental authority,as well as the sacredness and impartiality of Buddhism,it has effectively prevented corruption and malpractice during the reincarnation process.
文摘电感储能型脉冲电源是目前电磁发射技术的研究热点,提升装置储能密度对于推动电感储能型脉冲电源的应用和研究具有重要意义。本文介绍了一种适用于电感储能型脉冲电源的名为meat grinder with SECT的新电路,并对该电路进行了详细的原理分析,指出该电路兼具电流关断能力强和拓扑结构简单的优点。通过仿真将meat grinder with SECT和STRETCH meat grinder、STRETCH meat grinder with ICCOS这两种主流电路进行了性能比较,发现meat grinder with SECT电路在保持电气性能不变的同时,成本最低,体积最小。基于meat grinder with SECT电路,本文研制了50k J电感储能型脉冲电源并进行了试验测试,测试结果达到了预期目标。
基金supported by the Fund for Creative Research Groups of China,National Natural Science Foundation of China(No.81421005)the Specialized Research Fund of the Doctoral Program of Higher Education(SRFDP)+3 种基金Research Council Earmarked Research Grants(RCERG)(No.201300 96140001)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)partially supported by the National Natural Science Foundation of China(No.81274159)supported by New Century Excellent Talents in University(NCET-12-0976)
文摘The present study was designed to investigate the therapeutic effcts of Moutan Cortex(CM,root bark of Paeonia suffruticosa Andr) and Paeoniae Radix Rubra(PR,root of Paeonia veitchii Lynch) on metabolic disorders,focusing on the infuence of CM and PR on the obesity-related gut microbiota homeostasis.The diet-induced obese(DIO) mouse model was used to test the therapeutic effects of CM and PR.The mice were orally administered with CM and PR for 6 weeks,and oral glucose tolerance test(OGTT) and insulin tolerance test(ITT) were performed to evaluate the insulin sensitivity of the mice.Sterol-regulatory element binding proteins(SREBPs) and their target genes were measured by quantitative RT-PCR.High-throughput 16 S ribosomal RNA(16S rR NA) gene sequencing technology was used to determine the composition of gut microbiota,and the metabolites in serum were analyzed by GC-MS.Our results indicated that CM and PR combination alleviated obese and insulin resistance in the DIO mice,leading to increased glucose uptake and gene expression in muscle and liver,and down-regulated SREBPs and their target genes in liver.Interesting,neither the CM-PR extracts,nor the major components of CM and PR did not affect SREBPs activity in cultured cells.Meanwhile,CM and PR significantly modulated the gut microbiota of the high-fat diet(HFD) treated mice,similar to metformin,and CM-PR reversed the overall microbiota composition similar to the normal chow diet(NCD) treated mice.In conclusion,our results provide novel mechanisms of action for the effects of CM and PR in treating DIO-induced dysregulation of sugar and lipid metabolism.
