Ubiquitination,a critical post-translational modification,plays a pivotal role in fine tuning the immune responses of plants.The tomato(Solanum lycopersicum)suffers significant yield and quality losses caused by the d...Ubiquitination,a critical post-translational modification,plays a pivotal role in fine tuning the immune responses of plants.The tomato(Solanum lycopersicum)suffers significant yield and quality losses caused by the devastating pathogen Botrytis cinerea.We have discovered the role of SlRAE1,a gene encoding an E3 ubiquitin ligase,as a pivotal negative regulator of resistance to B.cinerea.SlRAE1 interacts with SlSKP1,a component of the SKP1–Cullin1–F-box(SCF)complex,to modulate the protein stability of the transcription factor SlWRKY1 through the 26S proteasome pathway.SlWRKY1 targets and inhibits the transcription of SlJAZ7,a suppressor of jasmonic acid(JA)signaling,thereby activating the JA-induced defense system and affecting tomato susceptibility to B.cinerea.The resistance enhancement observed with knock-out SlRAE1 was reduced when SlWRKY1 was also knocked out,highlighting SlWRKY1's role in SlRAE1's regulation of tomato defense against B.cinerea.Our findings elucidate the defense mechanism in tomato and suggest that targeting SlRAE1,by modulating SlWRKY1 stability,could help to develop resistant tomato varieties.These insights have broader implications for using gene-editing technologies to enhance crop defense against fungi.展开更多
生长素参与植物生长和发育诸多过程,调控众多生理反应,在植物整个生命周期中自始至终发挥着调节作用.研究生长素的作用机制,对深入认识植物生长发育的生理过程有着重要的意义.综述了与生长素信号转导途径相关的3类主要蛋白组分:生长素/...生长素参与植物生长和发育诸多过程,调控众多生理反应,在植物整个生命周期中自始至终发挥着调节作用.研究生长素的作用机制,对深入认识植物生长发育的生理过程有着重要的意义.综述了与生长素信号转导途径相关的3类主要蛋白组分:生长素/吲哚乙酸蛋白(auxin/indoleacetic acids proteins,Aux/IAAs)、生长素响应因子(auxin response factors,ARFs)和SCF(SKP1-CDC53/CUL1-F-box)复合体,及相关的SGT1(suppressor of the G2 allele of skp1)基因,并对生长素相关基因表达的模式及其生物学功能进行了总结.展开更多
基金supported by the National Natural Science Foundation of China (32172599)the Beijing Rural Revitalization Agricultural Science and Technology Project (NY2401080000)+3 种基金the Beijing Agriculture Innovation Consortium (BAIC012025)the support from the Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Cropsthe Engineering Research Center of Breeding and Propagation of Horticultural Cropsthe 2115 Talent Development Program of China Agricultural University
文摘Ubiquitination,a critical post-translational modification,plays a pivotal role in fine tuning the immune responses of plants.The tomato(Solanum lycopersicum)suffers significant yield and quality losses caused by the devastating pathogen Botrytis cinerea.We have discovered the role of SlRAE1,a gene encoding an E3 ubiquitin ligase,as a pivotal negative regulator of resistance to B.cinerea.SlRAE1 interacts with SlSKP1,a component of the SKP1–Cullin1–F-box(SCF)complex,to modulate the protein stability of the transcription factor SlWRKY1 through the 26S proteasome pathway.SlWRKY1 targets and inhibits the transcription of SlJAZ7,a suppressor of jasmonic acid(JA)signaling,thereby activating the JA-induced defense system and affecting tomato susceptibility to B.cinerea.The resistance enhancement observed with knock-out SlRAE1 was reduced when SlWRKY1 was also knocked out,highlighting SlWRKY1's role in SlRAE1's regulation of tomato defense against B.cinerea.Our findings elucidate the defense mechanism in tomato and suggest that targeting SlRAE1,by modulating SlWRKY1 stability,could help to develop resistant tomato varieties.These insights have broader implications for using gene-editing technologies to enhance crop defense against fungi.
文摘生长素参与植物生长和发育诸多过程,调控众多生理反应,在植物整个生命周期中自始至终发挥着调节作用.研究生长素的作用机制,对深入认识植物生长发育的生理过程有着重要的意义.综述了与生长素信号转导途径相关的3类主要蛋白组分:生长素/吲哚乙酸蛋白(auxin/indoleacetic acids proteins,Aux/IAAs)、生长素响应因子(auxin response factors,ARFs)和SCF(SKP1-CDC53/CUL1-F-box)复合体,及相关的SGT1(suppressor of the G2 allele of skp1)基因,并对生长素相关基因表达的模式及其生物学功能进行了总结.
