The age-hardening response,mechanical,and corrosion-resistant properties of AA7085 alloys with and without the addition of 0.3 wt.%scandium(Sc)were compared.Using advanced techniques such as aberration-corrected trans...The age-hardening response,mechanical,and corrosion-resistant properties of AA7085 alloys with and without the addition of 0.3 wt.%scandium(Sc)were compared.Using advanced techniques such as aberration-corrected transmission electron microscopy and first-principles calculations,the underlying micromechanisms of Sc microalloying were revealed.Results show that the increase in strength of the AA7085-Sc alloy is mainly attributed to the decreased Al grain size and increased number density of both Al_(3)Sc@Al_(3)(Sc,Zr)core−shell nanoparticles and Sc-containingη_(p) and GP−η_(p) nanoprecipitates.Strong strain fields and evident electron transfer from Zr to the neighboring matrix Al atoms exist at the Al_(3)Sc@Al_(3)(Sc,Zr)/Al interface.The Sc doping in GP−η_(p) andη_(p) suppresses the GP−η_(p)→η_(p) transformation.Modified corrosion resistance of the AA7085-Sc alloy compared with AA7085 alloy is associated with the fine grain boundary precipitates ofη_(p)hases and narrow precipitation free zone.The reasons of property changes of AA7085 alloy after Sc microalloying are explored based on the multiscale microstructural characterization.展开更多
[目的]明确36%烯肟·氟环唑SC对玉米小斑病的防治效果。[方法]以17%唑醚·氟环唑SC和30%肟菌·戊唑醇SC为对照药剂,在山东省潍坊市寒亭区高里街道河南村玉米田进行田间小区试验。[结果]36%烯肟·氟环唑SC81、108 g a.i....[目的]明确36%烯肟·氟环唑SC对玉米小斑病的防治效果。[方法]以17%唑醚·氟环唑SC和30%肟菌·戊唑醇SC为对照药剂,在山东省潍坊市寒亭区高里街道河南村玉米田进行田间小区试验。[结果]36%烯肟·氟环唑SC81、108 g a.i./hm^(2)处理对玉米小斑病效果优于对照药剂17%唑醚·氟环唑SC和30%肟菌·戊唑醇SC,54 g a.i./hm^(2)的36%烯肟·氟环唑SC处理对玉米小斑病效果与对照药剂无显著性差异。[结论]使用36%烯肟·氟环唑SC54~108 g a.i./hm^(2)在玉米小斑病发病前或发病初期茎叶均匀喷雾,间隔7~10 d再施用1次,可有效防治玉米小斑病。展开更多
为解析分泌型免疫球蛋白A(sIgA)中特有成分SC蛋白的结构和功能,通过PCR扩增猪SC基因的跨膜区,克隆到原核表达载体pET-28a(+)中,经测序验证后,诱导表达重组SC蛋白,用镍柱亲和层析纯化的重组SC蛋白免疫BALB/c小鼠,通过细胞融合技术筛选出...为解析分泌型免疫球蛋白A(sIgA)中特有成分SC蛋白的结构和功能,通过PCR扩增猪SC基因的跨膜区,克隆到原核表达载体pET-28a(+)中,经测序验证后,诱导表达重组SC蛋白,用镍柱亲和层析纯化的重组SC蛋白免疫BALB/c小鼠,通过细胞融合技术筛选出能够稳定分泌抗SC蛋白的细胞株,采用免疫荧光试验和Western-blot验证单抗特异性。结果表明,成功获得分子质量约72 k Da的重组SC蛋白;筛选出2株效价高达1∶32000的单抗细胞株(3F7A9和6F10E11)。结论:成功表达了SC蛋白,并制备出了抗SC蛋白的单克隆抗体,为研究SC蛋白的结构特性、功能作用以及构建高效、快速的sIgA抗体检测方法奠定了实验基础。展开更多
基金the support from the National Natural Science Foundation of China (Nos. U20A20274, 52061003)the Natural Science Foundation of Yunnan Province, China (No. 202301AT070209)the Science and Technology Major Project of Yunnan Province, China (No. 202102AG050017)。
文摘The age-hardening response,mechanical,and corrosion-resistant properties of AA7085 alloys with and without the addition of 0.3 wt.%scandium(Sc)were compared.Using advanced techniques such as aberration-corrected transmission electron microscopy and first-principles calculations,the underlying micromechanisms of Sc microalloying were revealed.Results show that the increase in strength of the AA7085-Sc alloy is mainly attributed to the decreased Al grain size and increased number density of both Al_(3)Sc@Al_(3)(Sc,Zr)core−shell nanoparticles and Sc-containingη_(p) and GP−η_(p) nanoprecipitates.Strong strain fields and evident electron transfer from Zr to the neighboring matrix Al atoms exist at the Al_(3)Sc@Al_(3)(Sc,Zr)/Al interface.The Sc doping in GP−η_(p) andη_(p) suppresses the GP−η_(p)→η_(p) transformation.Modified corrosion resistance of the AA7085-Sc alloy compared with AA7085 alloy is associated with the fine grain boundary precipitates ofη_(p)hases and narrow precipitation free zone.The reasons of property changes of AA7085 alloy after Sc microalloying are explored based on the multiscale microstructural characterization.
文摘[目的]明确36%烯肟·氟环唑SC对玉米小斑病的防治效果。[方法]以17%唑醚·氟环唑SC和30%肟菌·戊唑醇SC为对照药剂,在山东省潍坊市寒亭区高里街道河南村玉米田进行田间小区试验。[结果]36%烯肟·氟环唑SC81、108 g a.i./hm^(2)处理对玉米小斑病效果优于对照药剂17%唑醚·氟环唑SC和30%肟菌·戊唑醇SC,54 g a.i./hm^(2)的36%烯肟·氟环唑SC处理对玉米小斑病效果与对照药剂无显著性差异。[结论]使用36%烯肟·氟环唑SC54~108 g a.i./hm^(2)在玉米小斑病发病前或发病初期茎叶均匀喷雾,间隔7~10 d再施用1次,可有效防治玉米小斑病。
文摘为解析分泌型免疫球蛋白A(sIgA)中特有成分SC蛋白的结构和功能,通过PCR扩增猪SC基因的跨膜区,克隆到原核表达载体pET-28a(+)中,经测序验证后,诱导表达重组SC蛋白,用镍柱亲和层析纯化的重组SC蛋白免疫BALB/c小鼠,通过细胞融合技术筛选出能够稳定分泌抗SC蛋白的细胞株,采用免疫荧光试验和Western-blot验证单抗特异性。结果表明,成功获得分子质量约72 k Da的重组SC蛋白;筛选出2株效价高达1∶32000的单抗细胞株(3F7A9和6F10E11)。结论:成功表达了SC蛋白,并制备出了抗SC蛋白的单克隆抗体,为研究SC蛋白的结构特性、功能作用以及构建高效、快速的sIgA抗体检测方法奠定了实验基础。
