SARP Workshop on risk analysis of agroecological zonation and optimization of crop rotation was held at CNRRI’s research center on 24—27 Oct, 1995. Experts from Philippines, India, China, and IRRI were present in th...SARP Workshop on risk analysis of agroecological zonation and optimization of crop rotation was held at CNRRI’s research center on 24—27 Oct, 1995. Experts from Philippines, India, China, and IRRI were present in the workshop. Participants exchanged the research results on the field of land use, soil erosion. optimization of rice—wheat, rice—peanut, rice—corn cropping system in the different environments, risk analysis of rice based cropping system, nitrogen balance in rice based cropping system with system approach, demonstrated models used in their studies, and discussed the ongoing cooperative research.展开更多
Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due...Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitive binding capacity to mitochondrial complex I.The biosynthetic pathway of piericidin A1 has been reported in Streptomyces piomogeues var.Hangzhouwanensis,while the regulatory mechanism remains poorly understood.In this study,a Streptomyces antibiotic regulatory protein(SARP)family transcriptional regulator PieR was characterized.Genetic disruption and complementation manipulations revealed that PieR positively regulated the production of piericidin A1.Moreover,the overexpression of pieR contributed to the improvement of piericidin A1 productivity.The real-time quantitative PCR(RT-qPCR)was carried out and the data showed that pieR stimulated the transcription of all the biosynthesis-related genes for piericidin A1.In order to explore the regulatory mechanism,electrophoresis mobility shift assays(EMSA)and DNase I footprinting experiments have been conducted.A protected region covering 50 nucleotides within the upstream region of pieR was identified and two 5-nt direct repeat sequences(5′-CCGGA-3′)in the protected region were found.These findings,taken together,set stage for transcriptional control engineering in the view of optimizing piericidin A1 production and thus provide a viable potent route for the construction of strains with high productivity.展开更多
文摘SARP Workshop on risk analysis of agroecological zonation and optimization of crop rotation was held at CNRRI’s research center on 24—27 Oct, 1995. Experts from Philippines, India, China, and IRRI were present in the workshop. Participants exchanged the research results on the field of land use, soil erosion. optimization of rice—wheat, rice—peanut, rice—corn cropping system in the different environments, risk analysis of rice based cropping system, nitrogen balance in rice based cropping system with system approach, demonstrated models used in their studies, and discussed the ongoing cooperative research.
基金the National Natural Science Foundation of China(31630002,31700029,31770038,31470183,21661140002 and 31170085)the Ministry of Science and Technology+1 种基金Shanghai Pujiang Program from the Shanghai Municipal Council of Science and Technology(12PJD021)China Postdoctoral Science Foundation(2017M620151).
文摘Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitive binding capacity to mitochondrial complex I.The biosynthetic pathway of piericidin A1 has been reported in Streptomyces piomogeues var.Hangzhouwanensis,while the regulatory mechanism remains poorly understood.In this study,a Streptomyces antibiotic regulatory protein(SARP)family transcriptional regulator PieR was characterized.Genetic disruption and complementation manipulations revealed that PieR positively regulated the production of piericidin A1.Moreover,the overexpression of pieR contributed to the improvement of piericidin A1 productivity.The real-time quantitative PCR(RT-qPCR)was carried out and the data showed that pieR stimulated the transcription of all the biosynthesis-related genes for piericidin A1.In order to explore the regulatory mechanism,electrophoresis mobility shift assays(EMSA)and DNase I footprinting experiments have been conducted.A protected region covering 50 nucleotides within the upstream region of pieR was identified and two 5-nt direct repeat sequences(5′-CCGGA-3′)in the protected region were found.These findings,taken together,set stage for transcriptional control engineering in the view of optimizing piericidin A1 production and thus provide a viable potent route for the construction of strains with high productivity.
