The human retina,a complex and highly specialized structure,includes multiple cell types that work synergistically to generate and transmit visual signals.However,genetic predisposition or age-related degeneration can...The human retina,a complex and highly specialized structure,includes multiple cell types that work synergistically to generate and transmit visual signals.However,genetic predisposition or age-related degeneration can lead to retinal damage that severely impairs vision or causes blindness.Treatment options for retinal diseases are limited,and there is an urgent need for innovative therapeutic strategies.Cell and gene therapies are promising because of the efficacy of delivery systems that transport therapeutic genes to targeted retinal cells.Gene delivery systems hold great promise for treating retinal diseases by enabling the targeted delivery of therapeutic genes to affected cells or by converting endogenous cells into functional ones to facilitate nerve regeneration,potentially restoring vision.This review focuses on two principal categories of gene delivery vectors used in the treatment of retinal diseases:viral and non-viral systems.Viral vectors,including lentiviruses and adeno-associated viruses,exploit the innate ability of viruses to infiltrate cells,which is followed by the introduction of therapeutic genetic material into target cells for gene correction.Lentiviruses can accommodate exogenous genes up to 8 kb in length,but their mechanism of integration into the host genome presents insertion mutation risks.Conversely,adeno-associated viruses are safer,as they exist as episomes in the nucleus,yet their limited packaging capacity constrains their application to a narrower spectrum of diseases,which necessitates the exploration of alternative delivery methods.In parallel,progress has also occurred in the development of novel non-viral delivery systems,particularly those based on liposomal technology.Manipulation of the ratios of hydrophilic and hydrophobic molecules within liposomes and the development of new lipid formulations have led to the creation of advanced non-viral vectors.These innovative systems include solid lipid nanoparticles,polymer nanoparticles,dendrimers,polymeric micelles,and polymeric nanoparticles.Compared with their viral counterparts,non-viral delivery systems offer markedly enhanced loading capacities that enable the direct delivery of nucleic acids,mRNA,or protein molecules into cells.This bypasses the need for DNA transcription and processing,which significantly enhances therapeutic efficiency.Nevertheless,the immunogenic potential and accumulation toxicity associated with non-viral particulate systems necessitates continued optimization to reduce adverse effects in vivo.This review explores the various delivery systems for retinal therapies and retinal nerve regeneration,and details the characteristics,advantages,limitations,and clinical applications of each vector type.By systematically outlining these factors,our goal is to guide the selection of the optimal delivery tool for a specific retinal disease,which will enhance treatment efficacy and improve patient outcomes while paving the way for more effective and targeted therapeutic interventions.展开更多
Ⅰ. INTRODUCTION We have isolated from the extract of rat tectum a 30-kD protein, which is capable of maintaining survival and promoting growth of retinal ganglion cells of neonatal rats, namely retinal ganglion neuro...Ⅰ. INTRODUCTION We have isolated from the extract of rat tectum a 30-kD protein, which is capable of maintaining survival and promoting growth of retinal ganglion cells of neonatal rats, namely retinal ganglion neuronotrophic factor(RGNTF). With this RGNTF, we have raised展开更多
The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and contin...The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.展开更多
AIM:To explore the effect and mechanism of Lycium barbarum polysaccharide(LBP)inhibiting retinal neovascularization.METHODS:In vitro tests were performed on human retinal microvascular endothelial cells(HRECs)from thr...AIM:To explore the effect and mechanism of Lycium barbarum polysaccharide(LBP)inhibiting retinal neovascularization.METHODS:In vitro tests were performed on human retinal microvascular endothelial cells(HRECs)from three groups,including control group(normal oxygen),hypoxic group(hypoxia at 37℃,1%O_(2),5%CO_(2),and 94%N_(2)),and LBP group(hypoxic group with LBP 100μg/mL).In vivo experiments,C57 mice were divided into three groups:control group(normal rearing group),the oxygen-induced ischemic retinopathy(OIR)group,and the OIR with 50 mg/kg LBP group.Retinal neovascularization was observed by fluorescein angiography and quantified.Retinal thickness was evaluated by Hematoxylin and eosin(HE)stain.The expression of epidermal growth factor receptor(EGFR),phosphatidylinositol 3-kinase(PI3K),mammalian target of rapamycin(mTOR),phosphorylated mammalian target of rapamycin(p-mTOR),protein kinase B(AKT),phosphorylated protein kinase B(p-AKT),interleukin-1β(IL-1β),inducible nitric oxide synthase(iNOS),and tumor necrosis factor-α(TNF-α)in each group were analyzed by Western blot.IL-1βlevel in retina was analyzed using immunohistochemical staining.RESULTS:The increased area of neovascular clusters in OIR mice was significantly decreased by LBP.Retinal thickness of OIR mice was significantly thinner compared with normal oxygenated mice and was increased in LBP group.Compared with those in the hypoxic groups,Western blotting of HRECs and retinal tissues revealed that the expression of EGFR,PI3K,p-mTOR,p-AKT,IL-1β,iNOS,and TNF-αdecreased in the LBP group but was still greater than that in control group.Moreover,IL-1βwas reduced in retinal sections treated with LBP.In the scratch test,the cell migration of the hypoxic group was significantly greater than that of the control group,while LBP treatment attenuated this increase in migration.CONCLUSION:LBP reduces retinal neovascularization and inflammation in vivo and inhibits the migration of HRECs in vitro by regulating the EGFR/PI3K/Akt/mTOR signaling pathway.展开更多
Diabetic corneal neuropathy and diabetic retinopathy are ocular complications occurring in the context of diabetes mellitus.Diabetic corneal neuropathy refers to the progressive damage of corneal nerves.Diabetic retin...Diabetic corneal neuropathy and diabetic retinopathy are ocular complications occurring in the context of diabetes mellitus.Diabetic corneal neuropathy refers to the progressive damage of corneal nerves.Diabetic retinopathy has traditionally been considered as damage to the retinal microvasculature.However,growing evidence suggests that diabetic retinopathy is a complex neurovascular disorder resulting from dysfunction of the neurovascular unit,which includes both the retinal vascular structures and neural tissues.Diabetic retinopathy is one of the leading causes of blindness and is frequently screened for as part of diabetic ocular screening.However,diabetic corneal neuropathy is commonly overlooked and underdiagnosed,leading to severe ocular surface impairment.Several studies have found that these two conditions tend to occur together,and they share similarities in their pathogenesis pathways,being triggered by a status of chronic hyperglycemia.This review aims to discuss the interconnection between diabetic corneal neuropathy and diabetic retinopathy,whether diabetic corneal neuropathy precedes diabetic retinopathy,as well as the relation between the stage of diabetic retinopathy and the severity of corneal neuropathy.We also endeavor to explore the relevance of a corneal screening in diabetic eyes and the possibility of using corneal nerve measurements to monitor the progression of diabetic retinopathy.展开更多
Müller glia,as prominent glial cells within the retina,plays a significant role in maintaining retinal homeostasis in both healthy and diseased states.In lower vertebrates like zebrafish,these cells assume respon...Müller glia,as prominent glial cells within the retina,plays a significant role in maintaining retinal homeostasis in both healthy and diseased states.In lower vertebrates like zebrafish,these cells assume responsibility for spontaneous retinal regeneration,wherein endogenous Müller glia undergo proliferation,transform into Müller glia-derived progenitor cells,and subsequently regenerate the entire retina with restored functionality.Conversely,Müller glia in the mouse and human retina exhibit limited neural reprogramming.Müller glia reprogramming is thus a promising strategy for treating neurodegenerative ocular disorders.Müller glia reprogramming in mice has been accomplished with remarkable success,through various technologies.Advancements in molecular,genetic,epigenetic,morphological,and physiological evaluations have made it easier to document and investigate the Müller glia programming process in mice.Nevertheless,there remain issues that hinder improving reprogramming efficiency and maturity.Thus,understanding the reprogramming mechanism is crucial toward exploring factors that will improve Müller glia reprogramming efficiency,and for developing novel Müller glia reprogramming strategies.This review describes recent progress in relatively successful Müller glia reprogramming strategies.It also provides a basis for developing new Müller glia reprogramming strategies in mice,including epigenetic remodeling,metabolic modulation,immune regulation,chemical small-molecules regulation,extracellular matrix remodeling,and cell-cell fusion,to achieve Müller glia reprogramming in mice.展开更多
This study presents a real-time tracking algorithm derived from the retina algorithm,designed for the rapid,real-time tracking of straight-line particle trajectories.These trajectories are detected by pixel detectors ...This study presents a real-time tracking algorithm derived from the retina algorithm,designed for the rapid,real-time tracking of straight-line particle trajectories.These trajectories are detected by pixel detectors to localize single-event effects in two-dimensional space.Initially,we developed a retina algorithm to track the trajectory of a single heavy ion and achieved a positional accuracy of 40μm.This was accomplished by analyzing trajectory samples from the simulations using a pixel sensor with a 72×72 pixel array and an 83μm pixel pitch.Subsequently,we refined this approach to create an iterative retina algorithm for tracking multiple heavy-ion trajectories in single events.This iterative version demonstrated a tracking efficiency of over 97%,with a positional resolution comparable to that of single-track events.Furthermore,it exhibits significant parallelism,requires fewer resources,and is ideally suited for implementation in field-programmable gate arrays on board-level systems,facilitating real-time online trajectory tracking.展开更多
Photobiomodulation has been known to have potential medicinal effects for ages.It involves the use of specific wavelengths to target specific regions in the cell.Different health conditions have been reported to be tr...Photobiomodulation has been known to have potential medicinal effects for ages.It involves the use of specific wavelengths to target specific regions in the cell.Different health conditions have been reported to be treated with exposure to light such as cardiovascular conditions,inflammatory diseases,infectious diseases,and most importantly ocular diseases.This review specifically targets the treatment of retinal diseases including age-related macular degeneration,diabetic macular edema,myopia and acute retinal light injury with photobiomodulation.Red light is used in this therapy since this wavelength has lower frequency and hence minimal chance of causing any damage.Red light has the potential to penetrate cellular structures such as mitochondria and facilitate cellular processes.For ocular diseases,the target wavelength ranges between 630 to 800 nm.In most of the cases the primary target for red light is the cytochrome C oxidase enzyme in mitochondria,which alters the gene expression and promotes cellular energy production.Clinical evidence shows improvement of visual activity and reduction in thickness of retina post treatment.Future prospects of photobiomodulation involve target-specific treatment,combinational therapy to treat complex retinal diseases including gene therapy,and longitudinal studies to predict long-term efficacy and the chance of any recurrence in the patients.Hence the future of photobiomodulation holds significant potential in medicine especially in ocular diseases characterized by progress in research,technology,and clinical trials.展开更多
Optical Coherence Tomography(OCT)provides cross-sectional and three-dimensional reconstructions of the target tissue,allowing precise imaging and quantitative analysis of individual retinal layers.These images,based o...Optical Coherence Tomography(OCT)provides cross-sectional and three-dimensional reconstructions of the target tissue,allowing precise imaging and quantitative analysis of individual retinal layers.These images,based on optical inhomogeneities,reveal intricate cellular structures and are vital for tasks like retinal segmentation.The proposed study uses OCT images to identify significant differences in peripapillary retinal nerve fiber layer thickness.Incorporating spectral-domain analysis of OCT images significantly enhances the evaluation of Purtcher Retinopathy.To streamline this process,the study introduces a Context Encoding Deep Neural Network(CEDNN),which eliminates the time-consuming manual segmentation process while improving the accuracy of retinal layer thickness measurements.Despite the excellent performance of deep learning-based Convolutional Neural Networks(CNNs)in multiclass ocular fluid segmentation and lesion identification,certain challenges remain.Specifically,segmentation accuracy declines in regions with very tiny patches of subretinal fluid,often due to limited training data.The proposed CEDNN addresses these limitations by reducing processing time and enhancing accuracy.The approach incorporates advanced diffusion techniques in the 2D segmentation process using a gradient convergence field that accounts for the anisotropic nature of image features.Experimental results on public datasets and clinical OCT images demonstrate that the CEDNN approach achieves remarkable performance,with an accuracy of 99.3%,sensitivity of 99.4%,and specificity of 99%.Furthermore,the use of 3D representations of surface data outperforms traditional 2D surface estimates,enhancing segmentation quality.The system also incorporates temporal dimension estimation,making it feasible to forecast rapid disease progression.This advanced approach holds significant potential for improving retinal disease detection and analysis,setting a new benchmark in automated OCT-based diagnostics.展开更多
Microcystin-LR(MC-LR)is a highly toxic category of biotoxins that can damage eye development and retinal structure in zebrafish,while probiotics can largely benefit the function of the retina.Although they both act on...Microcystin-LR(MC-LR)is a highly toxic category of biotoxins that can damage eye development and retinal structure in zebrafish,while probiotics can largely benefit the function of the retina.Although they both act on the visual system,whether probiotics can alleviate the visual damage caused by MC-LR in fish and the underlying mechanisms remains unclear.In this study,we exposed adult zebrafish for 28 days at MC-LR concentrations of 0,2.20,and 22.00μg/L with or without the probiotic Lactobacillus rhamnosus in the diet.MC-LR exposure alone resulted in structural damage to the retina and abnormal phototropic behavior,whereas L.rhamnosus could alleviate these damages.Biochemical analyses showed thatMCLR-induced abnormalities in apoptosis of ocular cells,retinal inflammatory responses,neurotransmission,and phototransduction were restored in the L.rhamnosus treatment group,indicating L.rhamnosus alleviated MC-LR-induced defects in the visual system and dysfunctions.This study underlines the defensive role of probiotics in protecting the host from environmental pollutants,which may provide guidance for the application of probiotics in aquaculture.展开更多
AIM:To examined the effects of a high-fat diet(HFD)on retinal pathological changes and dysfunction using peroxisome proliferator-activated receptor-alpha(PPARα)knockout mice.METHODS:For four months,C57BL/6J and PPAR...AIM:To examined the effects of a high-fat diet(HFD)on retinal pathological changes and dysfunction using peroxisome proliferator-activated receptor-alpha(PPARα)knockout mice.METHODS:For four months,C57BL/6J and PPARαknockout mice received either HFD or a standard diet(SD).A fluorometric method was used to determine the retinal triglycerides.The retinal malondialdehyde(MDA)content was measured.Hematoxylin-eosin was used to evaluate retinal pathological changes.Protein expression was analyzed by Western blot and immunofluorescence,while mRNA expression was evaluated by quantitative reverse transcription-polymerase chain reaction.Electroretinogram was used to assess retinal function.RESULTS:HFD resulted in increased fatty acidβ-oxidation in the inner retina,particularly retinal ganglion cells(RGCs),as well as increased weight and accumulation of retinal triglyceride.Retinal fatty acid β-oxidation and triglyceride accumulation were affected by PPARα^(−/−)abnormalities.PPARαknockdown increased the infiltration and activation of inflammatory cells,as well as it upregulated the nuclear factor kappa B(NF-κB)signaling pathway and corresponding proinflammatory cytokine levels in the most retina subjected to the HFD.In the HFD mice,oxidative stress levels were elevated in the inner retina,particularly in the HFD PPARα^(−/−)mice.HFD-induced RGCs apoptosis initiation was exacerbated by PPARαdeficiency.Lastly,HFD feeding resulted in the lower amplitudes of scotopic a-wave,b-wave and photopic negative response(PhNR)wave,particularly in HFD PPARα^(−/−)mice.CONCLUSION:In HFD-fed mice retina,particularly in the inner retina,PPARα knockout increases lipid metabolic abnormalities,inflammatory responses,oxidative stress,apoptosis initiation and dysfunction.展开更多
Optical coherence tomography angiography(OCTA)is a powerful tool for non-invasive,label-free,three-dimensional vi-sualization of blood vessels down to the capillary level in vivo.However,its widespread usage is hinder...Optical coherence tomography angiography(OCTA)is a powerful tool for non-invasive,label-free,three-dimensional vi-sualization of blood vessels down to the capillary level in vivo.However,its widespread usage is hindered by the trade-off between transverse sampling rate and signal-to-noise ratio(SNR).This trade-off results in either a limited field of view(FOV)to maintain sampling density or loss of capillary details to fulfil FOV requirement.It also restricts microvascular quantifications,including flow velocimetry,which typically demand higher transverse sampling rate and SNR compared with standard qualitative OCTA.We introduce spectrally extended line field OCTA(SELF-OCTA),a cost-effective imag-ing modality that improves transverse sampling rate and SNR through spectrally encoded parallel sampling and in-creased signal acquired over longer periods,respectively.In the human skin and retina in vivo,we demonstrate its ad-vantages in achieving significantly extended FOV without sacrificing microvascular resolution,high sensitivity to slower flow without compromising FOV,and flow velocity quantification with the highest dynamic range,emphasizing that these features can be achieved with readily available and standard OCTA hardware settings.SELF-OCTA has the potential to make wide-field,high-resolution,quantitative angiographic imaging accessible to a wider population,thereby facilitating the early detection and follow-up of vascular-related diseases.展开更多
Autism spectrum disorder(ASD)represents a neurodevelopmental disorder that has been the focus of numerous studies on the central nervous system(CNS).The embryological origin of the brain and retina is shared,with the ...Autism spectrum disorder(ASD)represents a neurodevelopmental disorder that has been the focus of numerous studies on the central nervous system(CNS).The embryological origin of the brain and retina is shared,with the axons of retinal ganglion cells(RGC)developing into the optic nerves that enter the brain through the dorsal lateral geniculate nucleus(LGN)of the thalamus,LGN,and other visual cortices.Given the evidence that individuals with ASD exhibit impairments in the visual mechanisms,including deficits in emotional face recognition,and difficulty in maintaining gaze control as well as eye contact,some studies have documented retinal alterations in individuals with ASD.These have been identified through ophthalmic assessments,including optical coherence tomography(OCT),optical coherence tomography angiography(OCTA),and electroretinography(ERG).With the improvements in ASD animal models,it is possible to obtain a better understanding of vision dysfunction in ASD by analyzing the molecular mechanisms of retinal function and structure abnormalities.This review aims to provide a summary of the recent research on ocular alterations in ASD patients and animal models,intending to contribute to further investigation of the eye-brain connection and communication.展开更多
AIM:To evaluate retinal hemoglobin oxygen saturation in myopic eyes by scanning laser ophthalmoscope(SLO)and to assess its correlations with different severity of myopia.METHODS:Sixty-one eyes from 61 patients were in...AIM:To evaluate retinal hemoglobin oxygen saturation in myopic eyes by scanning laser ophthalmoscope(SLO)and to assess its correlations with different severity of myopia.METHODS:Sixty-one eyes from 61 patients were included and subdivided into three groups according to their refractive errors:high myopia group[20 eyes,spherical equivalent(SE)≤-6 D];low and moderate myopia(22 eyes,-6.0<SE≤-0.5 D);normal(19 eyes,-0.5<SE<+0.5 D).All subjects underwent SLO imaging with dual lasers(532 nm and 633 nm).The oxygen saturations of hemoglobin in arteries(SO_(2)A)and veins(SO_(2)V),and their differences(SO_(2)AV)were estimated from the optical densities of the vessels on the images at the two wavelengths.Pearson’s or Spearman’s rank correlation coefficient were calculated to assess the correlation between retinal hemoglobin oxygen saturation and refractive error/axial length(AL).RESULTS:For the retinal oxygen saturation,the SO_(2)V in high myopia group(73.21%±21.42%)was significantly higher than that in normal group(55.81%±21.69%)and low and moderate myopia group(56.88%±13.83%,P<0.05).The SE was significantly correlated with SO_(2)A(r=-0.30)and SO_(2)V(r=-0.36;P<0.05),and AL was also significantly correlated with SO_(2)A(r=0.27)and SO_(2)V(r=0.30;P<0.05).No significant correlations were found between SO_(2)AV and SE and AL(P>0.05).CONCLUSION:SO_(2)A and SO_(2)V increased in more myopic eye based on SLO measurements.Further studies are warranted to investigate the changes of retinal hemoglobin oxygen saturation in myopia with different methods.展开更多
BACKGROUND Silicone oil(SiO)migration to the central nervous system(CNS)is a rare complication of SiO tamponade after vitreo-retinal surgeries,it could masquerade hemorrhage on computed tomography neuro-imaging.Only l...BACKGROUND Silicone oil(SiO)migration to the central nervous system(CNS)is a rare complication of SiO tamponade after vitreo-retinal surgeries,it could masquerade hemorrhage on computed tomography neuro-imaging.Only limited cases were reported in the literature,certain intra-operative and post-operative ocular risk factors might contribute to the different extend of SiO migration in the CNS.AIM To study the risk factors for cerebral ventricular migration(CVM)on top of visual pathway migration(VPM).METHODS Conforming to the preferred reporting items for systematic reviews and metaanalyses guidelines,literature searches on PubMed,MEDLINE,EMBASE were performed on June 1,2024.Publications on SiO migration to CNS were included in this review.Non-English articles,and studies without neuro-imaging of the CNS were excluded.Patient demographics,SiO filled eyes'ocular characteristics and vitrectomy surgical details were extracted from included studies in this review.VPM and CVM were assigned as group 1 and group 2 respectively.Fisher's exact tests,Mann-Whitney U tests and binary logistic regression were performed.RESULTS Total 68 articles were obtained after searches,48 publications were included for analysis.Total 54 SiO filled eyes were analyzed.Post-vitrectomy intraocular pressure(IOP)was found to be significant in both Mann-Whitney U test(P=0.047)and binary logistic regression(P=0.012).Diabetic was found to be significant in binary logistic regression(P=0.037),but at borderline risk for CVM in Fisher's exact test(P=0.05).Other significant factors include longer SiO tamponade time(P=0.002 in Fisher's exact test)and visual acuity(P=0.011 in binary logistic regression).Optic nerve atrophy or disc cupping(P=1.00,P=0.790)and congenital optic disc anomalies(P=0.424)were all with P>0.05.CONCLUSION SiO migration to CNS is rare with limited case reports only.Our analysis of the existing literature demonstrated higher post-vitrectomy IOP was associated with CVM,followed by patients’diabetic status,longer SiO tamponade time and visual acuity.Optic nerve atrophy,disc cupping and congenital optic disc anomalies were not associated.Modifiable risk factors of post-vitrectomy IOP and SiO tamponade time should be closely monitored by vitreoretinal surgeons.Lower IOP target post-vitrectomy and earlier SiO removal surgeries should be arranged.展开更多
Dear Editor,Irreversible retinal damage can occur due to retinal degenerative(RD)diseases as well as injuries caused by accidents or devices.Laser devices can inflict permanent damage to the retina,leading to the loss...Dear Editor,Irreversible retinal damage can occur due to retinal degenerative(RD)diseases as well as injuries caused by accidents or devices.Laser devices can inflict permanent damage to the retina,leading to the loss of photoreceptors(PRs)and underlying retinal pigment epithelium(RPE),culminating in vision impairment.Since there is no effective treatment for permanent retinal injuries,replacing damaged PRs and RPE with corresponding healthy cells can be a suitable therapeutic approach.展开更多
Inflammation plays a crucial role in the regeneration of fish and avian retinas.However,how inflammation regulates Müller glia(MG)reprogramming remains unclear.Here,we used single-cell RNA sequencing to investiga...Inflammation plays a crucial role in the regeneration of fish and avian retinas.However,how inflammation regulates Müller glia(MG)reprogramming remains unclear.Here,we used single-cell RNA sequencing to investigate the cell heterogeneity and interactions of MG and immune cells in the regenerating zebrafish retina.We first showed that two types of quiescent MG(resting MG1 and MG2)reside in the uninjured retina.Following retinal injury,resting MG1 transitioned into an activated state expressing known reprogramming genes,while resting MG2 gave rise to rod progenitors.We further showed that retinal microglia can be categorized into three subtypes(microglia-1,microglia-2,and proliferative)and pseudotime analysis demonstrated dynamic changes in microglial status following retinal injury.Analysis of cell–cell interactions indicated extensive crosstalk between immune cells and MG,with many interactions shared among different immune cell types.Finally,we showed that inflammation activated Jak1–Stat3 signaling in MG,promoting their transition from a resting to an activated state.Our study reveals the cell heterogeneity and crosstalk of immune cells and MG in zebrafish retinal repair,and may provide valuable insights into future mammalian retina regeneration.展开更多
目的无人机摄像资料的分辨率直接影响目标识别与信息获取,所以摄像分辨率的提高具有重大意义。为了改善无人机侦察视频质量,针对目前无人机摄像、照相数据的特点,提出一种无人机侦察视频超分辨率重建方法。方法首先提出基于AGAST-Differ...目的无人机摄像资料的分辨率直接影响目标识别与信息获取,所以摄像分辨率的提高具有重大意义。为了改善无人机侦察视频质量,针对目前无人机摄像、照相数据的特点,提出一种无人机侦察视频超分辨率重建方法。方法首先提出基于AGAST-Difference与Fast Retina Keypoint(FREAK)的特征匹配算法对视频目标帧与相邻帧之间配准,然后提出匹配区域搜索方法找到目标帧与航片的对应关系,利用航片对视频帧进行高频补偿,最后采用凸集投影方法对补偿后视频帧进行迭代优化。结果基于AGAST-Difference与FREAK的特征匹配算法在尺度、旋转、视点等变化及运行速度上存在很大优势,匹配区域搜索方法使无人机视频的高频补偿连续性更好,凸集投影迭代优化提高了重建的边缘保持能力,与一种简单有效的视频序列超分辨率复原算法相比,本文算法重建质量提高约4 d B,运行速度提高约5倍。结论提出了一种针对无人机的视频超分辨率重建方法,分析了无人机视频超分辨率问题的核心所在,并且提出基于AGAST-Difference与FREAK的特征匹配算法与匹配区域搜索方法来解决图像配准与高频补偿问题。实验结果表明,本文算法强化了重建图像的一致性与保真度,特别是对图像边缘细节部分等效果极为明显,且处理速度更快。展开更多
Objective To observe the change of the neuropeptide pro-protein processing system in the ischemic retina ganglion cell-5(RGC-5) cells,pro-protein convertase-2(PC2),carboxypeptidase-E(CPE) and preproneuropeptide Y(prep...Objective To observe the change of the neuropeptide pro-protein processing system in the ischemic retina ganglion cell-5(RGC-5) cells,pro-protein convertase-2(PC2),carboxypeptidase-E(CPE) and preproneuropeptide Y(preproNPY) protein levels in the ischemic RGC-5 cells and conditioned medium were analyzed. Methods The RGC-5 cell was differentiated in 0.1 μmol/L staurosporine for 24 h and then stressed by different doses of oxygen and glucose deprivation(OGD). The acute or chronic OGD-induced cell death rates w...展开更多
基金Hongguang Wu,Both authors contributed equally to this work and share first authorshipLing Dong,Both authors contributed equally to this work and share first authorship。
文摘The human retina,a complex and highly specialized structure,includes multiple cell types that work synergistically to generate and transmit visual signals.However,genetic predisposition or age-related degeneration can lead to retinal damage that severely impairs vision or causes blindness.Treatment options for retinal diseases are limited,and there is an urgent need for innovative therapeutic strategies.Cell and gene therapies are promising because of the efficacy of delivery systems that transport therapeutic genes to targeted retinal cells.Gene delivery systems hold great promise for treating retinal diseases by enabling the targeted delivery of therapeutic genes to affected cells or by converting endogenous cells into functional ones to facilitate nerve regeneration,potentially restoring vision.This review focuses on two principal categories of gene delivery vectors used in the treatment of retinal diseases:viral and non-viral systems.Viral vectors,including lentiviruses and adeno-associated viruses,exploit the innate ability of viruses to infiltrate cells,which is followed by the introduction of therapeutic genetic material into target cells for gene correction.Lentiviruses can accommodate exogenous genes up to 8 kb in length,but their mechanism of integration into the host genome presents insertion mutation risks.Conversely,adeno-associated viruses are safer,as they exist as episomes in the nucleus,yet their limited packaging capacity constrains their application to a narrower spectrum of diseases,which necessitates the exploration of alternative delivery methods.In parallel,progress has also occurred in the development of novel non-viral delivery systems,particularly those based on liposomal technology.Manipulation of the ratios of hydrophilic and hydrophobic molecules within liposomes and the development of new lipid formulations have led to the creation of advanced non-viral vectors.These innovative systems include solid lipid nanoparticles,polymer nanoparticles,dendrimers,polymeric micelles,and polymeric nanoparticles.Compared with their viral counterparts,non-viral delivery systems offer markedly enhanced loading capacities that enable the direct delivery of nucleic acids,mRNA,or protein molecules into cells.This bypasses the need for DNA transcription and processing,which significantly enhances therapeutic efficiency.Nevertheless,the immunogenic potential and accumulation toxicity associated with non-viral particulate systems necessitates continued optimization to reduce adverse effects in vivo.This review explores the various delivery systems for retinal therapies and retinal nerve regeneration,and details the characteristics,advantages,limitations,and clinical applications of each vector type.By systematically outlining these factors,our goal is to guide the selection of the optimal delivery tool for a specific retinal disease,which will enhance treatment efficacy and improve patient outcomes while paving the way for more effective and targeted therapeutic interventions.
文摘Ⅰ. INTRODUCTION We have isolated from the extract of rat tectum a 30-kD protein, which is capable of maintaining survival and promoting growth of retinal ganglion cells of neonatal rats, namely retinal ganglion neuronotrophic factor(RGNTF). With this RGNTF, we have raised
基金supported by the National Natural Science Foundation of China,Nos.81901156(to ZZ),82271200(to ZZ),82171308(to XC)the Fundamental Research Funds for the Central Universities,No.xzy012022035(to ZZ)+1 种基金the Natural Science Foundation of Shaanxi Province,Nos.2021JM-261(to QK),2023-YBSF-303(to ZZ)Traditional Chinese Medicine Project of Shaanxi Province,No.2019-ZZ-JC047(to QK)。
文摘The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.
基金Supported by the Tianjin Health Research Project(No.ZC20069No.TJWJ2022MS040)+1 种基金the Foundation of the Committee of Integrated Traditional Chinese and Western Medicine(No.2021011)the Science and Technology Foundation of Tianjin Eye Hospital(No.YKYB1901).
文摘AIM:To explore the effect and mechanism of Lycium barbarum polysaccharide(LBP)inhibiting retinal neovascularization.METHODS:In vitro tests were performed on human retinal microvascular endothelial cells(HRECs)from three groups,including control group(normal oxygen),hypoxic group(hypoxia at 37℃,1%O_(2),5%CO_(2),and 94%N_(2)),and LBP group(hypoxic group with LBP 100μg/mL).In vivo experiments,C57 mice were divided into three groups:control group(normal rearing group),the oxygen-induced ischemic retinopathy(OIR)group,and the OIR with 50 mg/kg LBP group.Retinal neovascularization was observed by fluorescein angiography and quantified.Retinal thickness was evaluated by Hematoxylin and eosin(HE)stain.The expression of epidermal growth factor receptor(EGFR),phosphatidylinositol 3-kinase(PI3K),mammalian target of rapamycin(mTOR),phosphorylated mammalian target of rapamycin(p-mTOR),protein kinase B(AKT),phosphorylated protein kinase B(p-AKT),interleukin-1β(IL-1β),inducible nitric oxide synthase(iNOS),and tumor necrosis factor-α(TNF-α)in each group were analyzed by Western blot.IL-1βlevel in retina was analyzed using immunohistochemical staining.RESULTS:The increased area of neovascular clusters in OIR mice was significantly decreased by LBP.Retinal thickness of OIR mice was significantly thinner compared with normal oxygenated mice and was increased in LBP group.Compared with those in the hypoxic groups,Western blotting of HRECs and retinal tissues revealed that the expression of EGFR,PI3K,p-mTOR,p-AKT,IL-1β,iNOS,and TNF-αdecreased in the LBP group but was still greater than that in control group.Moreover,IL-1βwas reduced in retinal sections treated with LBP.In the scratch test,the cell migration of the hypoxic group was significantly greater than that of the control group,while LBP treatment attenuated this increase in migration.CONCLUSION:LBP reduces retinal neovascularization and inflammation in vivo and inhibits the migration of HRECs in vitro by regulating the EGFR/PI3K/Akt/mTOR signaling pathway.
文摘Diabetic corneal neuropathy and diabetic retinopathy are ocular complications occurring in the context of diabetes mellitus.Diabetic corneal neuropathy refers to the progressive damage of corneal nerves.Diabetic retinopathy has traditionally been considered as damage to the retinal microvasculature.However,growing evidence suggests that diabetic retinopathy is a complex neurovascular disorder resulting from dysfunction of the neurovascular unit,which includes both the retinal vascular structures and neural tissues.Diabetic retinopathy is one of the leading causes of blindness and is frequently screened for as part of diabetic ocular screening.However,diabetic corneal neuropathy is commonly overlooked and underdiagnosed,leading to severe ocular surface impairment.Several studies have found that these two conditions tend to occur together,and they share similarities in their pathogenesis pathways,being triggered by a status of chronic hyperglycemia.This review aims to discuss the interconnection between diabetic corneal neuropathy and diabetic retinopathy,whether diabetic corneal neuropathy precedes diabetic retinopathy,as well as the relation between the stage of diabetic retinopathy and the severity of corneal neuropathy.We also endeavor to explore the relevance of a corneal screening in diabetic eyes and the possibility of using corneal nerve measurements to monitor the progression of diabetic retinopathy.
基金supported by the National Natural Science Foundation of China,No.31930068National Key Research and Development Program of China,Nos.2018YFA0107302 and 2021YFA1101203(all to HX).
文摘Müller glia,as prominent glial cells within the retina,plays a significant role in maintaining retinal homeostasis in both healthy and diseased states.In lower vertebrates like zebrafish,these cells assume responsibility for spontaneous retinal regeneration,wherein endogenous Müller glia undergo proliferation,transform into Müller glia-derived progenitor cells,and subsequently regenerate the entire retina with restored functionality.Conversely,Müller glia in the mouse and human retina exhibit limited neural reprogramming.Müller glia reprogramming is thus a promising strategy for treating neurodegenerative ocular disorders.Müller glia reprogramming in mice has been accomplished with remarkable success,through various technologies.Advancements in molecular,genetic,epigenetic,morphological,and physiological evaluations have made it easier to document and investigate the Müller glia programming process in mice.Nevertheless,there remain issues that hinder improving reprogramming efficiency and maturity.Thus,understanding the reprogramming mechanism is crucial toward exploring factors that will improve Müller glia reprogramming efficiency,and for developing novel Müller glia reprogramming strategies.This review describes recent progress in relatively successful Müller glia reprogramming strategies.It also provides a basis for developing new Müller glia reprogramming strategies in mice,including epigenetic remodeling,metabolic modulation,immune regulation,chemical small-molecules regulation,extracellular matrix remodeling,and cell-cell fusion,to achieve Müller glia reprogramming in mice.
基金supported by the National Natural Science Foundation of China(No.12205224)the Research Foundation of Education Bureau of Hubei Province China(No.Q20221703)+1 种基金the National Natural Science Foundation of China(Nos.12035006,U2032140)the National Key Research and Development Program of China(No.2020YFE0202000)。
文摘This study presents a real-time tracking algorithm derived from the retina algorithm,designed for the rapid,real-time tracking of straight-line particle trajectories.These trajectories are detected by pixel detectors to localize single-event effects in two-dimensional space.Initially,we developed a retina algorithm to track the trajectory of a single heavy ion and achieved a positional accuracy of 40μm.This was accomplished by analyzing trajectory samples from the simulations using a pixel sensor with a 72×72 pixel array and an 83μm pixel pitch.Subsequently,we refined this approach to create an iterative retina algorithm for tracking multiple heavy-ion trajectories in single events.This iterative version demonstrated a tracking efficiency of over 97%,with a positional resolution comparable to that of single-track events.Furthermore,it exhibits significant parallelism,requires fewer resources,and is ideally suited for implementation in field-programmable gate arrays on board-level systems,facilitating real-time online trajectory tracking.
文摘Photobiomodulation has been known to have potential medicinal effects for ages.It involves the use of specific wavelengths to target specific regions in the cell.Different health conditions have been reported to be treated with exposure to light such as cardiovascular conditions,inflammatory diseases,infectious diseases,and most importantly ocular diseases.This review specifically targets the treatment of retinal diseases including age-related macular degeneration,diabetic macular edema,myopia and acute retinal light injury with photobiomodulation.Red light is used in this therapy since this wavelength has lower frequency and hence minimal chance of causing any damage.Red light has the potential to penetrate cellular structures such as mitochondria and facilitate cellular processes.For ocular diseases,the target wavelength ranges between 630 to 800 nm.In most of the cases the primary target for red light is the cytochrome C oxidase enzyme in mitochondria,which alters the gene expression and promotes cellular energy production.Clinical evidence shows improvement of visual activity and reduction in thickness of retina post treatment.Future prospects of photobiomodulation involve target-specific treatment,combinational therapy to treat complex retinal diseases including gene therapy,and longitudinal studies to predict long-term efficacy and the chance of any recurrence in the patients.Hence the future of photobiomodulation holds significant potential in medicine especially in ocular diseases characterized by progress in research,technology,and clinical trials.
基金funded by the Deanship of Research and Graduate Studies at King Khalid University through small group research under grant number RGP1/180/46.
文摘Optical Coherence Tomography(OCT)provides cross-sectional and three-dimensional reconstructions of the target tissue,allowing precise imaging and quantitative analysis of individual retinal layers.These images,based on optical inhomogeneities,reveal intricate cellular structures and are vital for tasks like retinal segmentation.The proposed study uses OCT images to identify significant differences in peripapillary retinal nerve fiber layer thickness.Incorporating spectral-domain analysis of OCT images significantly enhances the evaluation of Purtcher Retinopathy.To streamline this process,the study introduces a Context Encoding Deep Neural Network(CEDNN),which eliminates the time-consuming manual segmentation process while improving the accuracy of retinal layer thickness measurements.Despite the excellent performance of deep learning-based Convolutional Neural Networks(CNNs)in multiclass ocular fluid segmentation and lesion identification,certain challenges remain.Specifically,segmentation accuracy declines in regions with very tiny patches of subretinal fluid,often due to limited training data.The proposed CEDNN addresses these limitations by reducing processing time and enhancing accuracy.The approach incorporates advanced diffusion techniques in the 2D segmentation process using a gradient convergence field that accounts for the anisotropic nature of image features.Experimental results on public datasets and clinical OCT images demonstrate that the CEDNN approach achieves remarkable performance,with an accuracy of 99.3%,sensitivity of 99.4%,and specificity of 99%.Furthermore,the use of 3D representations of surface data outperforms traditional 2D surface estimates,enhancing segmentation quality.The system also incorporates temporal dimension estimation,making it feasible to forecast rapid disease progression.This advanced approach holds significant potential for improving retinal disease detection and analysis,setting a new benchmark in automated OCT-based diagnostics.
基金supported by the National Key Research and Development Program of China(No.2023YFD2400900)the National Natural Science Foundation of China(Nos.32171619 and 32201388)+2 种基金the Youth project of the Natural Science Foundation of Hubei Province(No.2021CFB243)Hubei Province Excellent Young and Middle aged Science and Technology Innovation Team Project(No.T2022028)the Youth Talent Project of Science and Technology Research Program of Hubei Provincial Department of Education(No.Q20212503)。
文摘Microcystin-LR(MC-LR)is a highly toxic category of biotoxins that can damage eye development and retinal structure in zebrafish,while probiotics can largely benefit the function of the retina.Although they both act on the visual system,whether probiotics can alleviate the visual damage caused by MC-LR in fish and the underlying mechanisms remains unclear.In this study,we exposed adult zebrafish for 28 days at MC-LR concentrations of 0,2.20,and 22.00μg/L with or without the probiotic Lactobacillus rhamnosus in the diet.MC-LR exposure alone resulted in structural damage to the retina and abnormal phototropic behavior,whereas L.rhamnosus could alleviate these damages.Biochemical analyses showed thatMCLR-induced abnormalities in apoptosis of ocular cells,retinal inflammatory responses,neurotransmission,and phototransduction were restored in the L.rhamnosus treatment group,indicating L.rhamnosus alleviated MC-LR-induced defects in the visual system and dysfunctions.This study underlines the defensive role of probiotics in protecting the host from environmental pollutants,which may provide guidance for the application of probiotics in aquaculture.
基金Supported by the Anhui Medical University Research Fund(No.2023xkj035)National Natural Science Foundation Incubation Program Project of the Second Affiliated Hospital of Anhui Medical University(No.2023GQFY05)the Key Research and Development Technology project of Anhui Province(No.2022j11020013).
文摘AIM:To examined the effects of a high-fat diet(HFD)on retinal pathological changes and dysfunction using peroxisome proliferator-activated receptor-alpha(PPARα)knockout mice.METHODS:For four months,C57BL/6J and PPARαknockout mice received either HFD or a standard diet(SD).A fluorometric method was used to determine the retinal triglycerides.The retinal malondialdehyde(MDA)content was measured.Hematoxylin-eosin was used to evaluate retinal pathological changes.Protein expression was analyzed by Western blot and immunofluorescence,while mRNA expression was evaluated by quantitative reverse transcription-polymerase chain reaction.Electroretinogram was used to assess retinal function.RESULTS:HFD resulted in increased fatty acidβ-oxidation in the inner retina,particularly retinal ganglion cells(RGCs),as well as increased weight and accumulation of retinal triglyceride.Retinal fatty acid β-oxidation and triglyceride accumulation were affected by PPARα^(−/−)abnormalities.PPARαknockdown increased the infiltration and activation of inflammatory cells,as well as it upregulated the nuclear factor kappa B(NF-κB)signaling pathway and corresponding proinflammatory cytokine levels in the most retina subjected to the HFD.In the HFD mice,oxidative stress levels were elevated in the inner retina,particularly in the HFD PPARα^(−/−)mice.HFD-induced RGCs apoptosis initiation was exacerbated by PPARαdeficiency.Lastly,HFD feeding resulted in the lower amplitudes of scotopic a-wave,b-wave and photopic negative response(PhNR)wave,particularly in HFD PPARα^(−/−)mice.CONCLUSION:In HFD-fed mice retina,particularly in the inner retina,PPARα knockout increases lipid metabolic abnormalities,inflammatory responses,oxidative stress,apoptosis initiation and dysfunction.
基金support from the Ministry of Education Singapore under its Academic Research Funding Tier 2 grant(MOE-T2EP30120-0001)Academic Research Funding Tier 1 grant(RG35/22)+1 种基金Singapore National Medical Research Council CS-NIG grant(MOH-CNIG24jan-0004)Guangzhou National Laboratory grant(GZNL2025C03014).
文摘Optical coherence tomography angiography(OCTA)is a powerful tool for non-invasive,label-free,three-dimensional vi-sualization of blood vessels down to the capillary level in vivo.However,its widespread usage is hindered by the trade-off between transverse sampling rate and signal-to-noise ratio(SNR).This trade-off results in either a limited field of view(FOV)to maintain sampling density or loss of capillary details to fulfil FOV requirement.It also restricts microvascular quantifications,including flow velocimetry,which typically demand higher transverse sampling rate and SNR compared with standard qualitative OCTA.We introduce spectrally extended line field OCTA(SELF-OCTA),a cost-effective imag-ing modality that improves transverse sampling rate and SNR through spectrally encoded parallel sampling and in-creased signal acquired over longer periods,respectively.In the human skin and retina in vivo,we demonstrate its ad-vantages in achieving significantly extended FOV without sacrificing microvascular resolution,high sensitivity to slower flow without compromising FOV,and flow velocity quantification with the highest dynamic range,emphasizing that these features can be achieved with readily available and standard OCTA hardware settings.SELF-OCTA has the potential to make wide-field,high-resolution,quantitative angiographic imaging accessible to a wider population,thereby facilitating the early detection and follow-up of vascular-related diseases.
基金Supported by Jiangsu Department of Science and Technology Key R&D Program(No.BE2023777)the Jiangsu Commission of Health(No.H2022185)Jiangsu Province Hospital(the First Affiliated Hospital of Nanjing Medical University)Clinical Capacity Enhancement Project(No.JSPH-MB-2023-18).
文摘Autism spectrum disorder(ASD)represents a neurodevelopmental disorder that has been the focus of numerous studies on the central nervous system(CNS).The embryological origin of the brain and retina is shared,with the axons of retinal ganglion cells(RGC)developing into the optic nerves that enter the brain through the dorsal lateral geniculate nucleus(LGN)of the thalamus,LGN,and other visual cortices.Given the evidence that individuals with ASD exhibit impairments in the visual mechanisms,including deficits in emotional face recognition,and difficulty in maintaining gaze control as well as eye contact,some studies have documented retinal alterations in individuals with ASD.These have been identified through ophthalmic assessments,including optical coherence tomography(OCT),optical coherence tomography angiography(OCTA),and electroretinography(ERG).With the improvements in ASD animal models,it is possible to obtain a better understanding of vision dysfunction in ASD by analyzing the molecular mechanisms of retinal function and structure abnormalities.This review aims to provide a summary of the recent research on ocular alterations in ASD patients and animal models,intending to contribute to further investigation of the eye-brain connection and communication.
基金Supported by Shantou Science and Technology Project(No.190917155269927)2020 Li Ka Shing Foundation Cross-Disciplinary Research Grant(No.2020LKSFG06B).
文摘AIM:To evaluate retinal hemoglobin oxygen saturation in myopic eyes by scanning laser ophthalmoscope(SLO)and to assess its correlations with different severity of myopia.METHODS:Sixty-one eyes from 61 patients were included and subdivided into three groups according to their refractive errors:high myopia group[20 eyes,spherical equivalent(SE)≤-6 D];low and moderate myopia(22 eyes,-6.0<SE≤-0.5 D);normal(19 eyes,-0.5<SE<+0.5 D).All subjects underwent SLO imaging with dual lasers(532 nm and 633 nm).The oxygen saturations of hemoglobin in arteries(SO_(2)A)and veins(SO_(2)V),and their differences(SO_(2)AV)were estimated from the optical densities of the vessels on the images at the two wavelengths.Pearson’s or Spearman’s rank correlation coefficient were calculated to assess the correlation between retinal hemoglobin oxygen saturation and refractive error/axial length(AL).RESULTS:For the retinal oxygen saturation,the SO_(2)V in high myopia group(73.21%±21.42%)was significantly higher than that in normal group(55.81%±21.69%)and low and moderate myopia group(56.88%±13.83%,P<0.05).The SE was significantly correlated with SO_(2)A(r=-0.30)and SO_(2)V(r=-0.36;P<0.05),and AL was also significantly correlated with SO_(2)A(r=0.27)and SO_(2)V(r=0.30;P<0.05).No significant correlations were found between SO_(2)AV and SE and AL(P>0.05).CONCLUSION:SO_(2)A and SO_(2)V increased in more myopic eye based on SLO measurements.Further studies are warranted to investigate the changes of retinal hemoglobin oxygen saturation in myopia with different methods.
文摘BACKGROUND Silicone oil(SiO)migration to the central nervous system(CNS)is a rare complication of SiO tamponade after vitreo-retinal surgeries,it could masquerade hemorrhage on computed tomography neuro-imaging.Only limited cases were reported in the literature,certain intra-operative and post-operative ocular risk factors might contribute to the different extend of SiO migration in the CNS.AIM To study the risk factors for cerebral ventricular migration(CVM)on top of visual pathway migration(VPM).METHODS Conforming to the preferred reporting items for systematic reviews and metaanalyses guidelines,literature searches on PubMed,MEDLINE,EMBASE were performed on June 1,2024.Publications on SiO migration to CNS were included in this review.Non-English articles,and studies without neuro-imaging of the CNS were excluded.Patient demographics,SiO filled eyes'ocular characteristics and vitrectomy surgical details were extracted from included studies in this review.VPM and CVM were assigned as group 1 and group 2 respectively.Fisher's exact tests,Mann-Whitney U tests and binary logistic regression were performed.RESULTS Total 68 articles were obtained after searches,48 publications were included for analysis.Total 54 SiO filled eyes were analyzed.Post-vitrectomy intraocular pressure(IOP)was found to be significant in both Mann-Whitney U test(P=0.047)and binary logistic regression(P=0.012).Diabetic was found to be significant in binary logistic regression(P=0.037),but at borderline risk for CVM in Fisher's exact test(P=0.05).Other significant factors include longer SiO tamponade time(P=0.002 in Fisher's exact test)and visual acuity(P=0.011 in binary logistic regression).Optic nerve atrophy or disc cupping(P=1.00,P=0.790)and congenital optic disc anomalies(P=0.424)were all with P>0.05.CONCLUSION SiO migration to CNS is rare with limited case reports only.Our analysis of the existing literature demonstrated higher post-vitrectomy IOP was associated with CVM,followed by patients’diabetic status,longer SiO tamponade time and visual acuity.Optic nerve atrophy,disc cupping and congenital optic disc anomalies were not associated.Modifiable risk factors of post-vitrectomy IOP and SiO tamponade time should be closely monitored by vitreoretinal surgeons.Lower IOP target post-vitrectomy and earlier SiO removal surgeries should be arranged.
基金supported by the National Eye Institute,National Institute of Health,Bethesda,Maryland,USA[CIRM DISC1-09912(BBT),NIH EY031144(BBT),R01 EY031834,EY-017337]the USC Ophthalmology Core(NIH P30EY029220,an unrestricted grant to the USC Department of Ophthalmology from RPB)+1 种基金the BrightFocus Foundation(M2016186,BBT)support from a Research to Prevent Blindness(RPB,New York,NY,USA)unrestricted grant to the UCI Department of Ophthalmology.
文摘Dear Editor,Irreversible retinal damage can occur due to retinal degenerative(RD)diseases as well as injuries caused by accidents or devices.Laser devices can inflict permanent damage to the retina,leading to the loss of photoreceptors(PRs)and underlying retinal pigment epithelium(RPE),culminating in vision impairment.Since there is no effective treatment for permanent retinal injuries,replacing damaged PRs and RPE with corresponding healthy cells can be a suitable therapeutic approach.
基金supported by the National Natural Science Foundation of China,Nos.81970820(to HX),31771644(to JL),31930068(to JL),82371176(to JL),81801331(to LC)National Key Research and Development Project of China.Nos.2017YFA0104100(to JL),2017YFA0701304(to HX)+1 种基金Shanghai Yangzhi Rehabilitation Hospital(Shanghai Sunshine Rehabilitation Center)Talent Introduction Plan,No.KYPT202204(to LC)the Fundamental Research Funds for the Central Universities,No.22120230292(to JL)。
文摘Inflammation plays a crucial role in the regeneration of fish and avian retinas.However,how inflammation regulates Müller glia(MG)reprogramming remains unclear.Here,we used single-cell RNA sequencing to investigate the cell heterogeneity and interactions of MG and immune cells in the regenerating zebrafish retina.We first showed that two types of quiescent MG(resting MG1 and MG2)reside in the uninjured retina.Following retinal injury,resting MG1 transitioned into an activated state expressing known reprogramming genes,while resting MG2 gave rise to rod progenitors.We further showed that retinal microglia can be categorized into three subtypes(microglia-1,microglia-2,and proliferative)and pseudotime analysis demonstrated dynamic changes in microglial status following retinal injury.Analysis of cell–cell interactions indicated extensive crosstalk between immune cells and MG,with many interactions shared among different immune cell types.Finally,we showed that inflammation activated Jak1–Stat3 signaling in MG,promoting their transition from a resting to an activated state.Our study reveals the cell heterogeneity and crosstalk of immune cells and MG in zebrafish retinal repair,and may provide valuable insights into future mammalian retina regeneration.
文摘目的无人机摄像资料的分辨率直接影响目标识别与信息获取,所以摄像分辨率的提高具有重大意义。为了改善无人机侦察视频质量,针对目前无人机摄像、照相数据的特点,提出一种无人机侦察视频超分辨率重建方法。方法首先提出基于AGAST-Difference与Fast Retina Keypoint(FREAK)的特征匹配算法对视频目标帧与相邻帧之间配准,然后提出匹配区域搜索方法找到目标帧与航片的对应关系,利用航片对视频帧进行高频补偿,最后采用凸集投影方法对补偿后视频帧进行迭代优化。结果基于AGAST-Difference与FREAK的特征匹配算法在尺度、旋转、视点等变化及运行速度上存在很大优势,匹配区域搜索方法使无人机视频的高频补偿连续性更好,凸集投影迭代优化提高了重建的边缘保持能力,与一种简单有效的视频序列超分辨率复原算法相比,本文算法重建质量提高约4 d B,运行速度提高约5倍。结论提出了一种针对无人机的视频超分辨率重建方法,分析了无人机视频超分辨率问题的核心所在,并且提出基于AGAST-Difference与FREAK的特征匹配算法与匹配区域搜索方法来解决图像配准与高频补偿问题。实验结果表明,本文算法强化了重建图像的一致性与保真度,特别是对图像边缘细节部分等效果极为明显,且处理速度更快。
基金supported by Guangdong Pharmaceutical University Grant (No. 2005SMK22) and Key-Teacher Training Grant.
文摘Objective To observe the change of the neuropeptide pro-protein processing system in the ischemic retina ganglion cell-5(RGC-5) cells,pro-protein convertase-2(PC2),carboxypeptidase-E(CPE) and preproneuropeptide Y(preproNPY) protein levels in the ischemic RGC-5 cells and conditioned medium were analyzed. Methods The RGC-5 cell was differentiated in 0.1 μmol/L staurosporine for 24 h and then stressed by different doses of oxygen and glucose deprivation(OGD). The acute or chronic OGD-induced cell death rates w...
