Foliar uptake of airborne metal(loid)s plays a crucial role in metal(loid)accumulation in plant organs and is influ-enced by the size and emission sources of aerosols.Given the high enrichment of toxic metal(loid)s in...Foliar uptake of airborne metal(loid)s plays a crucial role in metal(loid)accumulation in plant organs and is influ-enced by the size and emission sources of aerosols.Given the high enrichment of toxic metal(loid)s in submicron-scale particulates(PM1),this study established a PM1 exposure system to examine airborne metal(loid)accu-mulation and foliar physiological responses in Oryza sativa L.The results showed that the concentrations of Cu,Zn,As,Pb,and Cd in the leaves and grains were influenced not only by the airborne metal(loid)levels but also by the specific nature of the PM1 particles.The quantitative model for PM1-associated Pb entry into leaf tissue indicated that foliar Pb accumulation was primarily driven by particle adhesion,followed by hydrophilic pene-tration and trans-stomatal liquid film migration,accounting for 87%–89%of the total accumulation.The strong hygroscopicity and high Pb activity of PM1 emitted from waste incineration(WI)increased the Pb absorption coefficient via the hydrophilic and liquid film migration pathway.In contrast,the high hydrophobicity of PM1 from coal burning(CB)led to greater retention of Pb on leaf surfaces.Both foliar reactive oxygen metabolism and photosynthesis indices were sensitive to air pollution.Foliar metal(loid)accumulation and airborne PM1 concentration accounted for the variance in physiological responses in rice leaves.Our results also indicated that Pb was the key element in PM1 emissions from both coal burning(CB)and waste incineration(WI)responsible for significant physiological changes in rice leaves.展开更多
Accumulating evidence indicates that antibiotic exposure may lead to impaired vaccine responses1-4;however,the mechanisms underlying this association remain poorly understood.Here we prospectively followed 191 healthy...Accumulating evidence indicates that antibiotic exposure may lead to impaired vaccine responses1-4;however,the mechanisms underlying this association remain poorly understood.Here we prospectively followed 191 healthy,vaginally born,term infants from birth to 15 months,using a systems vaccinology approach to assess the effects of antibiotic exposure on immune responses to vaccination.Exposure to direct neonatal but not intrapartum antibiotics was associated with significantly lower antibody titres against various polysaccharides in the 13-valent pneumococcal conjugate vaccine and the Haemophilus influenzae type b polyribosylribitol phosphate and diphtheria toxoid antigens in the combined 6-in-1 Infanrix Hexa vaccine at 7 months of age.Blood from infants exposed to neonatal antibiotics had an inflammatory transcriptional profile before vaccination;in addition,faecal metagenomics showed reduced abundance of Bifidobacterium species in these infants at the time of vaccination,which was correlated with reduced vaccine antibody titres 6 months later.In preclinical models,responses to the 13-valent pneumococcal conjugate vaccine were strongly dependent on an intact microbiota but could be restored in germ-free mice by administering a consortium of Bifidobacterium species or a probiotic already widely used in neonatal units.Our data suggest that microbiota-targeted interventions could mitigate the detrimental effects of early-life antibiotics on vaccine immunogenicity.展开更多
Fibers with deformation-triggered responses are essential for smart textiles and wearable electronics.Here,smart core-shell elastomer fibers with a conductive core and a liquid crystal elastomer shell showing simultan...Fibers with deformation-triggered responses are essential for smart textiles and wearable electronics.Here,smart core-shell elastomer fibers with a conductive core and a liquid crystal elastomer shell showing simultaneous resistance and color responses are designed and prepared.The conductive core is consisted of interconnected liquid metal nanodroplets dispersed in a polymer matrix and the elastomer shell is made of cholesteric liquid crystals.When stretched,the fiber resistance increases as the interconnected pathways of liquid metal nanodroplets along the fiber axis become narrower,and the selective reflection color from the fiber surface blueshifts since the cholesteric pitch decreases.The smart elastomer fibers could be woven into smart textiles and respond to various mechanical deformations,including stretching,bending,compression and twisting.The average resistance change is 51%under 100%strain and its variation is smaller than 4%over 500 cycles,showing remarkable fatigue resistance.The simultaneous resistance and color responses to mechanical deformations make the fibers attractive for broad applications,such as flexible electronics.展开更多
Being caught in a flood is incredibly dangerous.Like many other natural disasters,floods can occur with little or no warning.Flash floods move quickly and have strong currents.They are known to rip(扯)trees out of the...Being caught in a flood is incredibly dangerous.Like many other natural disasters,floods can occur with little or no warning.Flash floods move quickly and have strong currents.They are known to rip(扯)trees out of the ground,destroy buildings and cause bridges to collapse.展开更多
The Nyctereutes procyonoides is highly regarded in the farming and leather industries because of the high value of its fur,which renders artificial feeding a crucial aspect.However,high-fat diets have always been asso...The Nyctereutes procyonoides is highly regarded in the farming and leather industries because of the high value of its fur,which renders artificial feeding a crucial aspect.However,high-fat diets have always been associated with a variety of digestive disorders.This study aimed to investigate the impact of high-fat diets on the gut microbiota and the mechanisms of gut damage in Nyctereutes procyonoides.16S rRNA sequencing demonstrated that high-fat diets caused diarrhea and intestinal damage through alterations in the gut microbiota:a decrease in the abundance of Firmicutes,an increase in the abundance of Proteobacteria and Actinobacteria,and an increase in the abundance of Enterococcaceae,Escherichia coli-Shigella,Clostridium and Lactobacillus.Subsequently,changes in metabolic path-ways,such as amino and fatty acid pathways,were identified by KEGG and COG enrichment analysis,and the TLR4/NF-κB/NLRP3 inflammatory signaling pathway was shown to be activated by high-fat diets.In addition,high-fat diets lead to the accumulation of ROS and MDA and reduce the activity of the antioxidant enzymes GSH-PX and SOD.C orrespondingly,the levels of proinflammatory cytokines(IL-6,IL-1βand TNF-α)were significantly increased,and the apoptosis and necrosis signaling pathways of colonic cells were detected,causing a dramatic decrease in the expression of intestinal tight junction proteins(Occludin,E-cadherin,ZO-1 and ZO-2).In conclusion,high-fat diets altered the structure of the Nyctereutes procyonoides gut microbiota community and led to colon damage.This study provides new insights into the intestinal health of Nyctereutes procyonoides.展开更多
The cardiopulmonary health of children may be affected by acute ozone(O3)exposure during physical activity[1];however,its effects in high-altitude regions such as the Xizang Plateau remain uncertain.In high-altitude a...The cardiopulmonary health of children may be affected by acute ozone(O3)exposure during physical activity[1];however,its effects in high-altitude regions such as the Xizang Plateau remain uncertain.In high-altitude areas,lower oxygen levels may cause children to experience shortness of breath or require increased respiratory effort during vigorous activities such as running.This could lead to increased pollutant inhalation,potentially elevating the burden on the cardiovascular system and triggering adverse reactions such as increased heart rate and elevated blood pressure.Furthermore,differences in physiological adaptation between Han children who have migrated to Xizang and Tibetan children who are native to the region may contribute to different reactions to environmental exposure[2].展开更多
Shape memory alloys(SMAs)are smart materials with superelasticity originating from a reversible stressinduced martensitic transformation(MT)accompanied by a significant electrical resistance change.However,the stress-...Shape memory alloys(SMAs)are smart materials with superelasticity originating from a reversible stressinduced martensitic transformation(MT)accompanied by a significant electrical resistance change.However,the stress-strain and resistance-stress relationships of typical NiTi wires are non-linear due to the stress plateau during the stress-induced MT.This limits the usage of these materials as pressure sensors.Herein,we propose a high-strength flexible sensor based on superelastic NiTi wires that achieves near-linear mechanical and electrical responses through a low-cost double-braided strategy.This microarchitectured strategy reduces or even eliminates stress plateau and it is demonstrated that the phase transformation of microfilaments can be controlled:regions with localized stress undergo the MT first,which is successively followed by the rest of the microfilament.This structure-dependent MT characteristic exhibits slim-hysteresis superelasticity and tunable low stiffness,and the braided wire shows improved flexibility.The double-braided NiTi microfilaments exhibit stable electrical properties and repeatability under approximately 600 MPa(8%strain)and can maintain stability over a wide temperature range(303-403 K).Moreover,a cross-grid flexible woven sensor array textile based on microfilaments is further developed to detect pressure distribution.This work provides insight into the design and application of SMAs in the field of flexible and functional fiber.展开更多
Magneto-electro-elastic(MEE)materials are widely utilized across various fields due to their multi-field coupling effects.Consequently,investigating the coupling behavior of MEE composite materials is of significant i...Magneto-electro-elastic(MEE)materials are widely utilized across various fields due to their multi-field coupling effects.Consequently,investigating the coupling behavior of MEE composite materials is of significant importance.The traditional finite element method(FEM)remains one of the primary approaches for addressing such issues.However,the application of FEM typically necessitates the use of a fine finite element mesh to accurately capture the heterogeneous properties of the materials and meet the required computational precision,which inevitably leads to a reduction in computational efficiency.To enhance the computational accuracy and efficiency of the FEM for heterogeneous multi-field coupling problems,this study presents the coupling magneto-electro-elastic multiscale finite element method(CM-MsFEM)for heterogeneous MEE structures.Unlike the conventional multiscale FEM(MsFEM),the proposed algorithm simultaneously constructs displacement,electric,and magnetic potential multiscale basis functions to address the heterogeneity of the corresponding parameters.The macroscale formulation of CM-MsFEM was derived,and the macroscale/microscale responses of the problems were obtained through up/downscaling calculations.Evaluation using numerical examples analyzing the transient behavior of heterogeneous MEE structures demonstrated that the proposed method outperforms traditional FEM in terms of both accuracy and computational efficiency,making it an appropriate choice for numerically modeling the dynamics of heterogeneous MEE structures.展开更多
Multi-axle heavy-duty vehicles(MHVs)are essential for military equipment transport due to their safety and stability.However,braking dynamic responses between MHVs and pavement systems still remain underexplored,parti...Multi-axle heavy-duty vehicles(MHVs)are essential for military equipment transport due to their safety and stability.However,braking dynamic responses between MHVs and pavement systems still remain underexplored,particularly regarding their complex load transfer mechanisms.This paper develops an enhanced model of a multi-axle heavy-duty vehicle(MHV)coupled with the uneven and flexible pavement.An advanced coupling iterative method is proposed to solve the highly dimensional equations of the MHV-pavement coupled system.The proposed method was validated through experimental tests,with characteristic parameters of vertical accelerations showing relative errors between 0.42%and 11.80%.The coupling effect and influence mechanism of the braking process are investigated by characteristic parameters of the dynamic responses.Additionally,the influences of braking conditions and pavement parameters are analyzed in time and frequency domains in order to reveal the vibration mechanisms of the coupled system.Moreover,this study establishes a theoretical foundation for monitoring pavement health via vehicle-mounted acceleration signals,which is necessary in military transportation.展开更多
Transformations of the world unseen in a century are unfolding at a faster pace.Changes of the world,of our times,and of the historical trajectory are taking place in ways like never before.As the new round of technol...Transformations of the world unseen in a century are unfolding at a faster pace.Changes of the world,of our times,and of the historical trajectory are taking place in ways like never before.As the new round of technological revolution and industrial transformation advances,the international power structure and world order are undergoing tectonic realignments and constantly being reshaped.The protracted Ukraine crisis and the escalating Palestinian-Israeli conflict exemplify the intensification of major-power strategic rivalries and geopolitical conflicts.At the same time,non-traditional security issues are getting severe and more deeply intertwined with traditional security.Challenges such as climate change,cybersecurity,energy security,and food security are becoming critical global issues which confront humanity with unprecedented threats and herald a new era of instability and transformation for the world’s development.展开更多
Under external disturbances,the shear mechanical responses and debonding failure mechanisms at anisotropic interfaces of anchoring system composed of multiphase media are inherently difficult to characterize due to th...Under external disturbances,the shear mechanical responses and debonding failure mechanisms at anisotropic interfaces of anchoring system composed of multiphase media are inherently difficult to characterize due to the concealment nature of interfacial interactions.This study establishes an equivalent shear model for a bolt-resin-rock anchoring system and conducts direct shear tests under dynamic normal load(DNL)boundary from both laboratory experiments and discrete element method(DEM)simulations.The research investigates the influence of normal dynamic load amplitude(An)and rock type on shear strength parameters,elucidating the evolutionary characteristics and underlying mechanisms of shear load and normal displacement fluctuations induced by cyclic normal loading,with maximum shear load decreasing by 36.81%to 46.94%as An increases from 10%to 70%when rock type varies from coal to limestone.Through analysis of strain field evolution,the critical impact of rock type on localization of shear failure surface is revealed,with systematic summarization of differentiated wear characteristics,failure modes,and key controlling factors associated with shear failure surface.Mesoscopic investigations enabled by DEM simulations uncover the nonuniform distribution of contact force chains within the material matrix and across the anisotropic interfaces under various DNL boundaries,clarify rock type dependent crack propagation pathways,and quantitatively assess the damage extent of shear failure surface,with the anisotropic interface damage factor increasing from 34.9%to 56.6%as An rises from 10%to 70%,and decreasing from 49.6%to 23.4%as rock type varies from coal to limestone.展开更多
Background:Published clinical trials have yielded controversial findings regarding the effects of sex on the benefits of immune checkpoint inhibitors(ICIs).Sex-associated differences in the efficacy of immunotherapy r...Background:Published clinical trials have yielded controversial findings regarding the effects of sex on the benefits of immune checkpoint inhibitors(ICIs).Sex-associated differences in the efficacy of immunotherapy remain an important,unresolved question.Methods:We investigated sex-biased molecular profiles across a multitude of biomarkers linked to immunotherapy responses.Multiomics data from major solid tumors in The Cancer Genome Atlas,with sufficient sample sizes(≥50 patients of each sex),were analyzed.Ninety-five molecular markers characterizing 4 distinct aspects of the tumor immune system were summarized and compared.The inverse probability of weights algorithm was used to generate well-balanced sex subgroups.Results:Our results showed that lung squamous cell carcinoma(LUSC),pancreatic adenocarcinoma,and liver hepatocellular carcinoma were the top 3 cancer types with extensive sex-biased biomarker profiles(31/95,15/95,and 14/95,respectively).Notably,although both were categorized as non–small cell lung carcinoma,LUSC harbored significantly more sex-biased immunological features than those of lung adenocarcinoma(p<0.01).We further explored the validity of this finding by analyzing ICI-responsive signatures and individual patient-level data for non–small cell lung carcinoma and found that sex had significant interaction effects on immunotherapy outcomes in LUSC(p_(interaction)<0.05),with women tending to derive greater benefits from ICIs than men.However,this difference was not apparent in the lung adenocarcinoma group(p_(interaction)=0.66),with men and women deriving comparable benefits.Conclusions:We systematically characterized sex-biased profiles of key molecular biomarkers predicting immunotherapy responses across solid tumors,which could pave the way for individualized therapeutic approaches for men and women.展开更多
Superior neutral or cationic dinuclear gold(Ⅰ)N-heterocyclic carbene(NHC)complexes with antitumor and tumor microenvironment regulation functions were developed by introducing an additional gold atom.The novel cation...Superior neutral or cationic dinuclear gold(Ⅰ)N-heterocyclic carbene(NHC)complexes with antitumor and tumor microenvironment regulation functions were developed by introducing an additional gold atom.The novel cationic dinuclear gold(Ⅰ)complex 4a(BF5-Au)with bis-NHC ligands exhibited potent anti-liver cancer capacity in vitro and in vivo.The Hyper7 sensor was first used to analyze the sites of reactive oxygen species(ROS)generation by BF5-Au,showing that ROS were preferably generated in mitochondria and endoplasmic reticulum.Mechanism studies showed that BF5-Au could induce immunogenic cell death(ICD)via ROS-driven endoplasmic reticulum stress(ERS).However,targeting a single type of immune cell seems insufficient to reverse the immunosuppressive circumstances.Further investigation indicated that BF5-Au could enhance antitumor immune responses by inducing ferroptosis and polarizing macrophages to M1-like types.Overall,BF5-Au could inhibit tumor growth and remodel the tumor microenvironment via ROS-driven ERS and ferroptosis,which is expected to be a promising chemoimmunotherapy for cancer treatment.展开更多
1.Introduction Planning and managing land resources requires the use of land use and land cover(LULC)maps,which provide vital information on the interactions between humans and the environment(Esfandeh et al.,2022;Pra...1.Introduction Planning and managing land resources requires the use of land use and land cover(LULC)maps,which provide vital information on the interactions between humans and the environment(Esfandeh et al.,2022;Pratic`o et al.,2021;Yao et al.,2022).The precision of LULC monitoring has increased due to developments in Earth observation and remote sensing,allowing for well-informed environmental management decision-making(Qian and Zhang,2022;Viana et al.,2019).展开更多
The coupling effects of rainfall,earthquake,and complex topographic and geological conditions complicate the dynamic responses and disasters of slope-tunnel systems.For this,the large-scale shaking table tests were ca...The coupling effects of rainfall,earthquake,and complex topographic and geological conditions complicate the dynamic responses and disasters of slope-tunnel systems.For this,the large-scale shaking table tests were carried out to explore the dynamic responses of steep bedding slope-tunnel system under the coupling effect of rainfall and earthquake.Results show that the slope surface and elevation amplification effect exhibit pronounced nonlinear change caused by the tunnel and weak interlayers.When seismic wave propagates to tunnels,the weak interlayers and rock intersecting areas present complex wave field distribution characteristics.The dynamic responses of the slope are influenced by the frequency,amplitude,and direction of seismic waves.The acceleration amplification coefficient initially rises and then falls as increasing seismic frequency,peaking at 20 Hz.Additionally,the seismic damage process of slope is categorized into elastic(2-3 m/s^(2)),elastoplastic(4-5 m/s^(2))and plastic damage stages(≥6.5 m/s^(2)).In elastic stage,ΔMPGA(ratio of acceleration amplification factor)increases with increasing seismic intensity,without obvious strain distribution change.In plastic stage,ΔMPGA begins to gradually plummet,and the strain is mainly distributed in the damaged area.The modes of seismic damage in the slope-tunnel system are mainly of tensile failure of the weak interlayer,cracking failure of tunnel lining,formation of persistent cracks on the slope crest and waist,development and outward shearing of the sliding mass,and buckling failure at the slope foot under extrusion of the upper rock body.This study can serve as a reference for predicting the failure modes of tunnel-slope system in strong seismic regions.展开更多
The time processes of photosynthetic induction responses to various irradiances in Korean pine (Pinus koraiensis) seedlings grown in open-light environments and in understory of forest were studied in an area near the...The time processes of photosynthetic induction responses to various irradiances in Korean pine (Pinus koraiensis) seedlings grown in open-light environments and in understory of forest were studied in an area near the Research Station of Changbai Mountain Forest Ecosystems, Jilin Province, China from July 15 to August 5, 1997. The results showed that at 200 靘olm-2s-1 photosynthetic photon flux density (PPFD) and 500 靘olm-2s-1 PPFD, the induction time for the photosynthetic rates of understory-grown seedlings to reach 50% and 90% steady-state net photosynthetic rates was longer than that of the open-grown seedlings. The induction responses of open-growth seedlings at 500 靘olm-2s-1 PPFD were slower than those at 200 靘olm-2s-1 PPFD, but it was the very reverse for understory-growth seedlings, which indicates that the photosynthetic induction times of Korean pine seedlings grown in the understory depended on the sunfleck intensity.展开更多
[Objective] The aim of this study was to explore the physiological and biochemical responses of Chinese cabbage to La and Zn. [Method] The effects of La and Zn on seed germination and seedling growth were explored by ...[Objective] The aim of this study was to explore the physiological and biochemical responses of Chinese cabbage to La and Zn. [Method] The effects of La and Zn on seed germination and seedling growth were explored by tissue culture method. [Result] La and Zn had little effects on seed germination rate, but significantly inhibited the growth of root and seedling, decreased their fresh weight at higher concentration; the inhibition of La proved higher on Chinese cabbage. The contents of chlorophyll and soluble protein increased at lower concentrations of La and Zn, but decreased at higher concentrations. With increasing doses of La and Zn, the activities of CAT and POD rose gradually, while SOD activity decreased at lower doses and increased at higher doses. Both of La and Zn would promote Of. producing and MDA accumulation. [Conclusion] In higher concentration, La was more poisonous than Zn. It is clear that the poisonous mechanism of La is similar to that of Zn. Hence, La is possibly a kind of new pollutant.展开更多
AIM: To study the modulation of glutamate on post-ischemic intestinal and cerebral inflammatory responses in a ischemic and excitotoxic rat model.METHODS: Adult male rats were subjected to bilateral carotid artery occ...AIM: To study the modulation of glutamate on post-ischemic intestinal and cerebral inflammatory responses in a ischemic and excitotoxic rat model.METHODS: Adult male rats were subjected to bilateral carotid artery occlusion for 15 min and injection of monosodium glutamate intraperitoneally, to decapitate them at selected time points. Tumor necrosis factor alpha (TNF-α) level and nuclear factor kappa B (NF-κB) activity were determined by enzyme-linked immunosorbant assay (ELISA) and electrophoretic mobility shift assay (EMSA), respectively.Hemodynamic parameters were monitored continuously during the whole process of cerebral ischemia and reperfusion.RESULTS: Monosodium glutamate (MSG) treated rats displayed statistically significant high levels of TNF-α in cerebral and intestinal tissuess within the first 6 h of ischemia. The rats with cerebral ischemia showed a minor decrease of TNF-α production in cerebral and intestinal tissuess. The rats with cerebral ischemia and treated with MSG displayed statistically significant low levels of TNF-α in cerebral and intestinal tissues. These results correlated significantly with NF-κB production calculated at the same intervals. During experiment, the mean blood pressure and heart rates in all groups were stable.CONCLUSION: Glutamate is involved in the mechanism of intestinal and cerebral inflammation responses. The effects of glutamate on cerebral and intestinal inflammatory responses after ischemia are up-regulated at the transcriptional level,through the NF-κB signal transduction pathway.展开更多
Habitat fragmentation poses a significant threat to bird communities, especially those in open and semi-open ecosystems such as steppes. This study investigates how steppe birds adapt to and utilize fragmented habitat...Habitat fragmentation poses a significant threat to bird communities, especially those in open and semi-open ecosystems such as steppes. This study investigates how steppe birds adapt to and utilize fragmented habitats by combining niche modeling with ecological trait analysis. We conducted standardized point surveys to examine the habitat preferences of 32 bird species in Inner Mongolia, China, and quantified their habitat niche parameters using the Outlying Mean Index (OMI). Our results reveal distinct habitat preferences among species, with some thriving in intact environments while others are better adapted to fragmented areas. Grassland species showed high specialization along the fragmentation gradient, while others exhibited adaptability to varying levels of fragmentation. Using a Generalized Additive Model (GAM), we identified three key traits influencing habitat occupancy: hand-wing index, body mass, and range size. Specifically, species with medium hand-wing indices, moderate body mass, and larger range sizes were more likely to occupy heavily fragmented habitats. These findings provide empirical evidence on how habitat fragmentation affects bird species in steppe ecosystems. The study highlights the importance of functional traits in understanding avian responses to habitat fragmentation and offers a foundation for developing effective conservation strategies to preserve biodiversity in fragmented landscapes.展开更多
基金supported by the National Natural Science Foundation of China(Nos.42077367 and 21677123).
文摘Foliar uptake of airborne metal(loid)s plays a crucial role in metal(loid)accumulation in plant organs and is influ-enced by the size and emission sources of aerosols.Given the high enrichment of toxic metal(loid)s in submicron-scale particulates(PM1),this study established a PM1 exposure system to examine airborne metal(loid)accu-mulation and foliar physiological responses in Oryza sativa L.The results showed that the concentrations of Cu,Zn,As,Pb,and Cd in the leaves and grains were influenced not only by the airborne metal(loid)levels but also by the specific nature of the PM1 particles.The quantitative model for PM1-associated Pb entry into leaf tissue indicated that foliar Pb accumulation was primarily driven by particle adhesion,followed by hydrophilic pene-tration and trans-stomatal liquid film migration,accounting for 87%–89%of the total accumulation.The strong hygroscopicity and high Pb activity of PM1 emitted from waste incineration(WI)increased the Pb absorption coefficient via the hydrophilic and liquid film migration pathway.In contrast,the high hydrophobicity of PM1 from coal burning(CB)led to greater retention of Pb on leaf surfaces.Both foliar reactive oxygen metabolism and photosynthesis indices were sensitive to air pollution.Foliar metal(loid)accumulation and airborne PM1 concentration accounted for the variance in physiological responses in rice leaves.Our results also indicated that Pb was the key element in PM1 emissions from both coal burning(CB)and waste incineration(WI)responsible for significant physiological changes in rice leaves.
文摘Accumulating evidence indicates that antibiotic exposure may lead to impaired vaccine responses1-4;however,the mechanisms underlying this association remain poorly understood.Here we prospectively followed 191 healthy,vaginally born,term infants from birth to 15 months,using a systems vaccinology approach to assess the effects of antibiotic exposure on immune responses to vaccination.Exposure to direct neonatal but not intrapartum antibiotics was associated with significantly lower antibody titres against various polysaccharides in the 13-valent pneumococcal conjugate vaccine and the Haemophilus influenzae type b polyribosylribitol phosphate and diphtheria toxoid antigens in the combined 6-in-1 Infanrix Hexa vaccine at 7 months of age.Blood from infants exposed to neonatal antibiotics had an inflammatory transcriptional profile before vaccination;in addition,faecal metagenomics showed reduced abundance of Bifidobacterium species in these infants at the time of vaccination,which was correlated with reduced vaccine antibody titres 6 months later.In preclinical models,responses to the 13-valent pneumococcal conjugate vaccine were strongly dependent on an intact microbiota but could be restored in germ-free mice by administering a consortium of Bifidobacterium species or a probiotic already widely used in neonatal units.Our data suggest that microbiota-targeted interventions could mitigate the detrimental effects of early-life antibiotics on vaccine immunogenicity.
基金supported by the National Natural Science Foundation of China(No.22278352)National Key Research and Development Program of China(No.2021YFC3001100)。
文摘Fibers with deformation-triggered responses are essential for smart textiles and wearable electronics.Here,smart core-shell elastomer fibers with a conductive core and a liquid crystal elastomer shell showing simultaneous resistance and color responses are designed and prepared.The conductive core is consisted of interconnected liquid metal nanodroplets dispersed in a polymer matrix and the elastomer shell is made of cholesteric liquid crystals.When stretched,the fiber resistance increases as the interconnected pathways of liquid metal nanodroplets along the fiber axis become narrower,and the selective reflection color from the fiber surface blueshifts since the cholesteric pitch decreases.The smart elastomer fibers could be woven into smart textiles and respond to various mechanical deformations,including stretching,bending,compression and twisting.The average resistance change is 51%under 100%strain and its variation is smaller than 4%over 500 cycles,showing remarkable fatigue resistance.The simultaneous resistance and color responses to mechanical deformations make the fibers attractive for broad applications,such as flexible electronics.
文摘Being caught in a flood is incredibly dangerous.Like many other natural disasters,floods can occur with little or no warning.Flash floods move quickly and have strong currents.They are known to rip(扯)trees out of the ground,destroy buildings and cause bridges to collapse.
文摘The Nyctereutes procyonoides is highly regarded in the farming and leather industries because of the high value of its fur,which renders artificial feeding a crucial aspect.However,high-fat diets have always been associated with a variety of digestive disorders.This study aimed to investigate the impact of high-fat diets on the gut microbiota and the mechanisms of gut damage in Nyctereutes procyonoides.16S rRNA sequencing demonstrated that high-fat diets caused diarrhea and intestinal damage through alterations in the gut microbiota:a decrease in the abundance of Firmicutes,an increase in the abundance of Proteobacteria and Actinobacteria,and an increase in the abundance of Enterococcaceae,Escherichia coli-Shigella,Clostridium and Lactobacillus.Subsequently,changes in metabolic path-ways,such as amino and fatty acid pathways,were identified by KEGG and COG enrichment analysis,and the TLR4/NF-κB/NLRP3 inflammatory signaling pathway was shown to be activated by high-fat diets.In addition,high-fat diets lead to the accumulation of ROS and MDA and reduce the activity of the antioxidant enzymes GSH-PX and SOD.C orrespondingly,the levels of proinflammatory cytokines(IL-6,IL-1βand TNF-α)were significantly increased,and the apoptosis and necrosis signaling pathways of colonic cells were detected,causing a dramatic decrease in the expression of intestinal tight junction proteins(Occludin,E-cadherin,ZO-1 and ZO-2).In conclusion,high-fat diets altered the structure of the Nyctereutes procyonoides gut microbiota community and led to colon damage.This study provides new insights into the intestinal health of Nyctereutes procyonoides.
基金supported by the National Key Research and Development Program of China(grant number 2022YFC3702604)National Natural Science Foundation of China(41977374).
文摘The cardiopulmonary health of children may be affected by acute ozone(O3)exposure during physical activity[1];however,its effects in high-altitude regions such as the Xizang Plateau remain uncertain.In high-altitude areas,lower oxygen levels may cause children to experience shortness of breath or require increased respiratory effort during vigorous activities such as running.This could lead to increased pollutant inhalation,potentially elevating the burden on the cardiovascular system and triggering adverse reactions such as increased heart rate and elevated blood pressure.Furthermore,differences in physiological adaptation between Han children who have migrated to Xizang and Tibetan children who are native to the region may contribute to different reactions to environmental exposure[2].
基金supported by the National Natural Science Foundation of China(Nos.52031005,52201224)the Natural Science Foundation of Shanghai(No.24ZR1438200)+1 种基金the Shanghai Academy of Spaceflight Technology Joint Research Fund(No.USCAST2023-19)the Equipment Development Depart-ment Huiyan Action.
文摘Shape memory alloys(SMAs)are smart materials with superelasticity originating from a reversible stressinduced martensitic transformation(MT)accompanied by a significant electrical resistance change.However,the stress-strain and resistance-stress relationships of typical NiTi wires are non-linear due to the stress plateau during the stress-induced MT.This limits the usage of these materials as pressure sensors.Herein,we propose a high-strength flexible sensor based on superelastic NiTi wires that achieves near-linear mechanical and electrical responses through a low-cost double-braided strategy.This microarchitectured strategy reduces or even eliminates stress plateau and it is demonstrated that the phase transformation of microfilaments can be controlled:regions with localized stress undergo the MT first,which is successively followed by the rest of the microfilament.This structure-dependent MT characteristic exhibits slim-hysteresis superelasticity and tunable low stiffness,and the braided wire shows improved flexibility.The double-braided NiTi microfilaments exhibit stable electrical properties and repeatability under approximately 600 MPa(8%strain)and can maintain stability over a wide temperature range(303-403 K).Moreover,a cross-grid flexible woven sensor array textile based on microfilaments is further developed to detect pressure distribution.This work provides insight into the design and application of SMAs in the field of flexible and functional fiber.
基金supported by the National Natural Science Foundation of China(Grant Nos.42102346,42172301).
文摘Magneto-electro-elastic(MEE)materials are widely utilized across various fields due to their multi-field coupling effects.Consequently,investigating the coupling behavior of MEE composite materials is of significant importance.The traditional finite element method(FEM)remains one of the primary approaches for addressing such issues.However,the application of FEM typically necessitates the use of a fine finite element mesh to accurately capture the heterogeneous properties of the materials and meet the required computational precision,which inevitably leads to a reduction in computational efficiency.To enhance the computational accuracy and efficiency of the FEM for heterogeneous multi-field coupling problems,this study presents the coupling magneto-electro-elastic multiscale finite element method(CM-MsFEM)for heterogeneous MEE structures.Unlike the conventional multiscale FEM(MsFEM),the proposed algorithm simultaneously constructs displacement,electric,and magnetic potential multiscale basis functions to address the heterogeneity of the corresponding parameters.The macroscale formulation of CM-MsFEM was derived,and the macroscale/microscale responses of the problems were obtained through up/downscaling calculations.Evaluation using numerical examples analyzing the transient behavior of heterogeneous MEE structures demonstrated that the proposed method outperforms traditional FEM in terms of both accuracy and computational efficiency,making it an appropriate choice for numerically modeling the dynamics of heterogeneous MEE structures.
基金National Defense Basic Scientific Research Program of China(Grant No.JCKY2021602B030).
文摘Multi-axle heavy-duty vehicles(MHVs)are essential for military equipment transport due to their safety and stability.However,braking dynamic responses between MHVs and pavement systems still remain underexplored,particularly regarding their complex load transfer mechanisms.This paper develops an enhanced model of a multi-axle heavy-duty vehicle(MHV)coupled with the uneven and flexible pavement.An advanced coupling iterative method is proposed to solve the highly dimensional equations of the MHV-pavement coupled system.The proposed method was validated through experimental tests,with characteristic parameters of vertical accelerations showing relative errors between 0.42%and 11.80%.The coupling effect and influence mechanism of the braking process are investigated by characteristic parameters of the dynamic responses.Additionally,the influences of braking conditions and pavement parameters are analyzed in time and frequency domains in order to reveal the vibration mechanisms of the coupled system.Moreover,this study establishes a theoretical foundation for monitoring pavement health via vehicle-mounted acceleration signals,which is necessary in military transportation.
文摘Transformations of the world unseen in a century are unfolding at a faster pace.Changes of the world,of our times,and of the historical trajectory are taking place in ways like never before.As the new round of technological revolution and industrial transformation advances,the international power structure and world order are undergoing tectonic realignments and constantly being reshaped.The protracted Ukraine crisis and the escalating Palestinian-Israeli conflict exemplify the intensification of major-power strategic rivalries and geopolitical conflicts.At the same time,non-traditional security issues are getting severe and more deeply intertwined with traditional security.Challenges such as climate change,cybersecurity,energy security,and food security are becoming critical global issues which confront humanity with unprecedented threats and herald a new era of instability and transformation for the world’s development.
基金support from the National Natural Science Foundation of China(Nos.51504247,52174092,51904290,and 52074259)the Natural Science Foundation of Jiangsu Province,China(No.BK20220157)+1 种基金the Fundamental Research Funds for the Central Universities,China(No.2022YCPY0202)the China University of Mining and Technology(CUMT)Open Sharing Fund for Large-scale Instruments and Equipment(No.DYGX-2025-47)is gratefully acknowledged.
文摘Under external disturbances,the shear mechanical responses and debonding failure mechanisms at anisotropic interfaces of anchoring system composed of multiphase media are inherently difficult to characterize due to the concealment nature of interfacial interactions.This study establishes an equivalent shear model for a bolt-resin-rock anchoring system and conducts direct shear tests under dynamic normal load(DNL)boundary from both laboratory experiments and discrete element method(DEM)simulations.The research investigates the influence of normal dynamic load amplitude(An)and rock type on shear strength parameters,elucidating the evolutionary characteristics and underlying mechanisms of shear load and normal displacement fluctuations induced by cyclic normal loading,with maximum shear load decreasing by 36.81%to 46.94%as An increases from 10%to 70%when rock type varies from coal to limestone.Through analysis of strain field evolution,the critical impact of rock type on localization of shear failure surface is revealed,with systematic summarization of differentiated wear characteristics,failure modes,and key controlling factors associated with shear failure surface.Mesoscopic investigations enabled by DEM simulations uncover the nonuniform distribution of contact force chains within the material matrix and across the anisotropic interfaces under various DNL boundaries,clarify rock type dependent crack propagation pathways,and quantitatively assess the damage extent of shear failure surface,with the anisotropic interface damage factor increasing from 34.9%to 56.6%as An rises from 10%to 70%,and decreasing from 49.6%to 23.4%as rock type varies from coal to limestone.
基金supported by grants from the Special Funding of China Postdoctoral Science Foundation(No.2022TQ0389)the National Natural Science Foundation of China(No.82303693)the National Postdoctoral Program for Innovative Talents(No.BX2021386)。
文摘Background:Published clinical trials have yielded controversial findings regarding the effects of sex on the benefits of immune checkpoint inhibitors(ICIs).Sex-associated differences in the efficacy of immunotherapy remain an important,unresolved question.Methods:We investigated sex-biased molecular profiles across a multitude of biomarkers linked to immunotherapy responses.Multiomics data from major solid tumors in The Cancer Genome Atlas,with sufficient sample sizes(≥50 patients of each sex),were analyzed.Ninety-five molecular markers characterizing 4 distinct aspects of the tumor immune system were summarized and compared.The inverse probability of weights algorithm was used to generate well-balanced sex subgroups.Results:Our results showed that lung squamous cell carcinoma(LUSC),pancreatic adenocarcinoma,and liver hepatocellular carcinoma were the top 3 cancer types with extensive sex-biased biomarker profiles(31/95,15/95,and 14/95,respectively).Notably,although both were categorized as non–small cell lung carcinoma,LUSC harbored significantly more sex-biased immunological features than those of lung adenocarcinoma(p<0.01).We further explored the validity of this finding by analyzing ICI-responsive signatures and individual patient-level data for non–small cell lung carcinoma and found that sex had significant interaction effects on immunotherapy outcomes in LUSC(p_(interaction)<0.05),with women tending to derive greater benefits from ICIs than men.However,this difference was not apparent in the lung adenocarcinoma group(p_(interaction)=0.66),with men and women deriving comparable benefits.Conclusions:We systematically characterized sex-biased profiles of key molecular biomarkers predicting immunotherapy responses across solid tumors,which could pave the way for individualized therapeutic approaches for men and women.
基金supported by the National Natural Science Foundation of China(No.82173684)the Priority Academic Program Development of Jiangsu Higher Education Institutions(Integration of Chinese and Western Medicine),High level key discipline construction project of the National Administration of Traditional Chinese Medicine-Resource Chemistry of Chinese Medicinal Materials(No.zyyzdxk-2023083)+1 种基金the Key R&D Program of Jiangsu Province(No.BE2023840)Yunnan Provincial Science and Technology Talent and Platform Program(No.202405AF140031)。
文摘Superior neutral or cationic dinuclear gold(Ⅰ)N-heterocyclic carbene(NHC)complexes with antitumor and tumor microenvironment regulation functions were developed by introducing an additional gold atom.The novel cationic dinuclear gold(Ⅰ)complex 4a(BF5-Au)with bis-NHC ligands exhibited potent anti-liver cancer capacity in vitro and in vivo.The Hyper7 sensor was first used to analyze the sites of reactive oxygen species(ROS)generation by BF5-Au,showing that ROS were preferably generated in mitochondria and endoplasmic reticulum.Mechanism studies showed that BF5-Au could induce immunogenic cell death(ICD)via ROS-driven endoplasmic reticulum stress(ERS).However,targeting a single type of immune cell seems insufficient to reverse the immunosuppressive circumstances.Further investigation indicated that BF5-Au could enhance antitumor immune responses by inducing ferroptosis and polarizing macrophages to M1-like types.Overall,BF5-Au could inhibit tumor growth and remodel the tumor microenvironment via ROS-driven ERS and ferroptosis,which is expected to be a promising chemoimmunotherapy for cancer treatment.
文摘1.Introduction Planning and managing land resources requires the use of land use and land cover(LULC)maps,which provide vital information on the interactions between humans and the environment(Esfandeh et al.,2022;Pratic`o et al.,2021;Yao et al.,2022).The precision of LULC monitoring has increased due to developments in Earth observation and remote sensing,allowing for well-informed environmental management decision-making(Qian and Zhang,2022;Viana et al.,2019).
基金supported by the National Natural Science Foundation of China (Grant No.52109125)the Natural Science Foundation of Jiangsu Province,China (Grant No.BK20231217)the Key Laboratory of Geomechanics and Geotechnical Engineering Safety,Chinese Academy of Sciences (Grant No.SKLGME023001).
文摘The coupling effects of rainfall,earthquake,and complex topographic and geological conditions complicate the dynamic responses and disasters of slope-tunnel systems.For this,the large-scale shaking table tests were carried out to explore the dynamic responses of steep bedding slope-tunnel system under the coupling effect of rainfall and earthquake.Results show that the slope surface and elevation amplification effect exhibit pronounced nonlinear change caused by the tunnel and weak interlayers.When seismic wave propagates to tunnels,the weak interlayers and rock intersecting areas present complex wave field distribution characteristics.The dynamic responses of the slope are influenced by the frequency,amplitude,and direction of seismic waves.The acceleration amplification coefficient initially rises and then falls as increasing seismic frequency,peaking at 20 Hz.Additionally,the seismic damage process of slope is categorized into elastic(2-3 m/s^(2)),elastoplastic(4-5 m/s^(2))and plastic damage stages(≥6.5 m/s^(2)).In elastic stage,ΔMPGA(ratio of acceleration amplification factor)increases with increasing seismic intensity,without obvious strain distribution change.In plastic stage,ΔMPGA begins to gradually plummet,and the strain is mainly distributed in the damaged area.The modes of seismic damage in the slope-tunnel system are mainly of tensile failure of the weak interlayer,cracking failure of tunnel lining,formation of persistent cracks on the slope crest and waist,development and outward shearing of the sliding mass,and buckling failure at the slope foot under extrusion of the upper rock body.This study can serve as a reference for predicting the failure modes of tunnel-slope system in strong seismic regions.
文摘The time processes of photosynthetic induction responses to various irradiances in Korean pine (Pinus koraiensis) seedlings grown in open-light environments and in understory of forest were studied in an area near the Research Station of Changbai Mountain Forest Ecosystems, Jilin Province, China from July 15 to August 5, 1997. The results showed that at 200 靘olm-2s-1 photosynthetic photon flux density (PPFD) and 500 靘olm-2s-1 PPFD, the induction time for the photosynthetic rates of understory-grown seedlings to reach 50% and 90% steady-state net photosynthetic rates was longer than that of the open-grown seedlings. The induction responses of open-growth seedlings at 500 靘olm-2s-1 PPFD were slower than those at 200 靘olm-2s-1 PPFD, but it was the very reverse for understory-growth seedlings, which indicates that the photosynthetic induction times of Korean pine seedlings grown in the understory depended on the sunfleck intensity.
基金Supported by National Natural Science Foundation of China(No.30900071)Start-up Foundation for Doctor Scientific Research in Shandong University of Technology(4041-406027)~~
文摘[Objective] The aim of this study was to explore the physiological and biochemical responses of Chinese cabbage to La and Zn. [Method] The effects of La and Zn on seed germination and seedling growth were explored by tissue culture method. [Result] La and Zn had little effects on seed germination rate, but significantly inhibited the growth of root and seedling, decreased their fresh weight at higher concentration; the inhibition of La proved higher on Chinese cabbage. The contents of chlorophyll and soluble protein increased at lower concentrations of La and Zn, but decreased at higher concentrations. With increasing doses of La and Zn, the activities of CAT and POD rose gradually, while SOD activity decreased at lower doses and increased at higher doses. Both of La and Zn would promote Of. producing and MDA accumulation. [Conclusion] In higher concentration, La was more poisonous than Zn. It is clear that the poisonous mechanism of La is similar to that of Zn. Hence, La is possibly a kind of new pollutant.
文摘AIM: To study the modulation of glutamate on post-ischemic intestinal and cerebral inflammatory responses in a ischemic and excitotoxic rat model.METHODS: Adult male rats were subjected to bilateral carotid artery occlusion for 15 min and injection of monosodium glutamate intraperitoneally, to decapitate them at selected time points. Tumor necrosis factor alpha (TNF-α) level and nuclear factor kappa B (NF-κB) activity were determined by enzyme-linked immunosorbant assay (ELISA) and electrophoretic mobility shift assay (EMSA), respectively.Hemodynamic parameters were monitored continuously during the whole process of cerebral ischemia and reperfusion.RESULTS: Monosodium glutamate (MSG) treated rats displayed statistically significant high levels of TNF-α in cerebral and intestinal tissuess within the first 6 h of ischemia. The rats with cerebral ischemia showed a minor decrease of TNF-α production in cerebral and intestinal tissuess. The rats with cerebral ischemia and treated with MSG displayed statistically significant low levels of TNF-α in cerebral and intestinal tissues. These results correlated significantly with NF-κB production calculated at the same intervals. During experiment, the mean blood pressure and heart rates in all groups were stable.CONCLUSION: Glutamate is involved in the mechanism of intestinal and cerebral inflammation responses. The effects of glutamate on cerebral and intestinal inflammatory responses after ischemia are up-regulated at the transcriptional level,through the NF-κB signal transduction pathway.
基金supported by the National Natural Science Foundation of China(No.32201304)the Fundamental Research Funds for the Central Universities(No.2412022QD026).
文摘Habitat fragmentation poses a significant threat to bird communities, especially those in open and semi-open ecosystems such as steppes. This study investigates how steppe birds adapt to and utilize fragmented habitats by combining niche modeling with ecological trait analysis. We conducted standardized point surveys to examine the habitat preferences of 32 bird species in Inner Mongolia, China, and quantified their habitat niche parameters using the Outlying Mean Index (OMI). Our results reveal distinct habitat preferences among species, with some thriving in intact environments while others are better adapted to fragmented areas. Grassland species showed high specialization along the fragmentation gradient, while others exhibited adaptability to varying levels of fragmentation. Using a Generalized Additive Model (GAM), we identified three key traits influencing habitat occupancy: hand-wing index, body mass, and range size. Specifically, species with medium hand-wing indices, moderate body mass, and larger range sizes were more likely to occupy heavily fragmented habitats. These findings provide empirical evidence on how habitat fragmentation affects bird species in steppe ecosystems. The study highlights the importance of functional traits in understanding avian responses to habitat fragmentation and offers a foundation for developing effective conservation strategies to preserve biodiversity in fragmented landscapes.
