Background This study investigated the potential impacts of increasing linoleic andα-linolenic acid intake during lactation and wean-to-breeding on subsequent reproduction of sows.A total of 309 sows(PIC Camborough L...Background This study investigated the potential impacts of increasing linoleic andα-linolenic acid intake during lactation and wean-to-breeding on subsequent reproduction of sows.A total of 309 sows(PIC Camborough L42)were balanced by parity(140 and 169 sows representing parity 1 to 2[P1-2]and 3 to 9[P3+],respectively)and assigned within parity to a 2×2 factorial arrangement.Factors included essential fatty acid(EFA)supplementation(control diets containing 1.2%linoleic and 0.15%α-linolenic acid or diets with 3.0%linoleic and 0.38%α-linolenic acid)and supplementation period(lactation or wean-to-breeding).Tallow(low EFA diets)or soybean oil(high EFA diets)were included at 4%in sorghum-soybean meal-wheat middlings-based diets to attain targeted EFA levels.Results High levels of EFA fed during lactation had no effect on feed intake or litter performance,but increased subsequent farrowing rate(P=0.027;82.1%vs.70.4%),tended to reduce the proportion of sows removed(P=0.070;12.4%vs.20.8%),decreased the number of total pigs born in the following litter(P=0.072;15.3 vs.16.2),and increased total pigs born alive per 100 sows weaned(P=0.062;1,122 vs.974),regardless of sow parity.Young sows(P1-2)consuming the high EFA diet during lactation displayed a shorter wean-to-estrus interval(P=0.035;4.2 vs.4.6),but P3+sows were unaffected.Increasing EFA intake for P3+sows,but not P1-2 sows,resulted in more sows bred by d 5(P=0.028;91.1%vs.81.7%)and more mummies in the subsequent litter(P=0.040;0.32 vs.0.16).Feeding increased EFA to P1-2 sows during the wean-to-breeding period decreased subsequent farrowing rate(P=0.042;72.0%vs.87.7%),and increased removal rate(P=0.003;28.8%vs.9.4%).Total pigs born alive per 100 sows weaned was reduced(P=0.007)in P1-2 sows when supplemented with EFA during wean-breeding(939 vs.1,149)but was not impacted in P3+sows(1,131 vs.982).Conclusions Supplemental EFA in lactation diets benefited subsequent reproduction of sows,regardless of parity.Increasing dietary levels of EFA during the wean-to-breeding period to younger sows negatively impacted subsequent reproduction.展开更多
Cytokinins are ancient hormones present across all kingdoms of life except archaea,although functional biosynthesis pathways have yet to be identified in animalia.Known for their roles in cell division and proliferati...Cytokinins are ancient hormones present across all kingdoms of life except archaea,although functional biosynthesis pathways have yet to be identified in animalia.Known for their roles in cell division and proliferation,cytokinins are critical to plant life,as they regulate various aspects of vegetative growth,stress response,and reproduction.In this review,we summarize literature from 2020 to 2025 pertaining to the cytokinin functions in plant reproduction.While general aspects of cytokinin’s role in plant reproduction have been addressed,we particularly focus on the role of cytokinin in reproductive systems due to recent work identifying their role as sex-determining factors in dioecious species in Salicaceae and other families,their role in determining flower sex in monoecious species,and their involvement in self-incompatibility response and asexual reproduction.展开更多
Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadens...Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadensis (L.) Nutt.) in the national economy. The trials were carried out in the experimental site of SamSU’s Botanic Garden, which is named after Sharof Rashidov. The study’s findings are based on the results obtained by planting cuttings of S. canadensis and E. macrophylla species of varying sizes and durations, as well as the cuttings’ roots and the impacts of growth chemicals.展开更多
Environmental fluctuations are a pervasive natural phenomenon that typically favor diverse maternal investment strategies.Organisms across various evolutionary lineages have acquired the ability to enter the dormancy ...Environmental fluctuations are a pervasive natural phenomenon that typically favor diverse maternal investment strategies.Organisms across various evolutionary lineages have acquired the ability to enter the dormancy state to tolerate conditions that are unsuitable for normal life activities.In cyclical parthenogenesis,sexual reproduction is generally linked with dormancy,thereby enhancing the long-term fitness of populations through positive feedback mechanisms.This study evaluates the impact of environmental variability on offspring population dynamics through sexual reproduction in the rotifer Brachionus calyciflorus.We found that mictic females(sexual females)exposed to low-predictability environments produced resting eggs with a sporadic hatching pattern,reduced hatching rate,and smaller sizes of resting egg,embryo,and stem females,while increasing variations in fertilization among individuals to maximize population fitness.Variations in population growth could be manifested in enhanced reproductive capacity 2–3 d post-hatching,along with differences in fecundity among individuals.This suggests that dormant eggs from unstable environments may prioritize offspring survival and adjust to increased fertilization among individuals.This adaptive strategy enables rotifer populations to rapidly adjust to environmental changes,thus avoiding extinction and thriving in diverse habitats.Our systematic study enhances the understanding of how environmental stability affects long-term population fitness in cyclical parthenogens.展开更多
In this paper,the author follows the trail of C.Malabou,Q.Meillassoux,and G.Deleuze and tries to test three philosophical concepts that seem to be particularly threatened in the era of automatic digital reproduction.T...In this paper,the author follows the trail of C.Malabou,Q.Meillassoux,and G.Deleuze and tries to test three philosophical concepts that seem to be particularly threatened in the era of automatic digital reproduction.These three concepts are plasticity(defended for many years by C.Malabou),contingency(reconstructed by Q.Meillassoux),and virtuality(developed by G.Deleuze).The main task of the text will be to reflect on which of these three concepts better protects our thinking against automation and stays faithful to the ideal of creativity.In what sense are plasticity,contingency,and the possibility of virtualization the a priori condition of any transformation,physical or intellectual,affective or conceptual metamorphosis?In what sense are these three concepts the only conditions for the survival of every living being?Would a being without contingency,plasticity,and disposition to virtualization simply be a dead being?展开更多
Objective:To evaluate mobile applications available for patients undergoing assisted reproduction and assess the extent of their clinical validation.Methods:A systematic search was conducted in the Apple App Store and...Objective:To evaluate mobile applications available for patients undergoing assisted reproduction and assess the extent of their clinical validation.Methods:A systematic search was conducted in the Apple App Store and Google Play between September 1,2023 and September 30,2023 to identify mobile applications related to assisted reproduction.Apps were evaluated using the mobile app rating scale(MARS).In parallel,a literature search of PubMed,Scopus,Embase,and Web of Science was performed to identify clinical studies related to mobile applications in assisted reproduction.Clinical validation status and MARS scores were recorded,and findings were synthesized to highlight the gap between commercially available apps and research-based evidence.Results:From 1143 apps screened,11 met the inclusion criteria.Mean MARS score across apps was 3.63,with Leeaf scoring the highest(4.60).However,only one application(Embie)was supported by published research.The literature research identified 13 relevant studies,mostly randomized controlled trials,cohort studies,or usability studies.While research-based apps demonstrated clinical utility(e.g.,MediEmo,PreLiFe,Patient Journey App),most were unavailable on app stores.This revealed a disconnect between research-backed applications and those accessible to patients.Conclusions:Although several mobile apps for assisted reproduction demonstrate high usability and quality,few are clinically validated.The lack of integration between research and practice highlights the need for stronger collaboration between researchers,developers,and policymakers to ensure that patients access safe and effective tools.展开更多
The paper is an introduction to the front-end pulse acquisition and the back-end pulse biomimetic reproduction system.This system is capable of faithfully replicating the complete pulse waveform collected at the front...The paper is an introduction to the front-end pulse acquisition and the back-end pulse biomimetic reproduction system.This system is capable of faithfully replicating the complete pulse waveform collected at the front end.Traditional Chinese Medicine(TCM)practitioners analyze and diagnose the pulse patterns at the replication end.Meanwhile,the obtained pulse waveforms are analyzed and learnt by a neural network based on key diagnostic points in TCM pulse taking,which enables the determination of the corresponding relationships between different pulse waveforms and various pulse patterns in TCM pulse taking.With the support of clinical samples,an auxiliary diagnostic system for TCM pulse patterns ensures the accuracy of pulse pattern replication.展开更多
Objective: Adolescent reproduction health (ARH) differs greatly based on religions, religious norms, culture, and ethnicities in the Indonesian context. The value of local wisdom was considered too binding, traditiona...Objective: Adolescent reproduction health (ARH) differs greatly based on religions, religious norms, culture, and ethnicities in the Indonesian context. The value of local wisdom was considered too binding, traditional, and outdated, particularly on ARH in agricultural areas. The purpose of the study was to identify the problems, challenges, and needs, specifically for ARH, in the agricultural area of Indonesia. Methods: A qualitative study was performed among 41 communities, including 28 village officials (VOs), 9 community health volunteers (CHVs), and 9 adolescents. The data were collected through in-depth interviews and FGD. An thematic analysis was used to describe the phenomena. Results: This research obtained results regarding ARH in terms of communication patterns, daily behavior, reproductive practices, and hygiene of adolescent reproductive organs, especially in agricultural areas. Conclusions: The differences in the perspective of ARH are influenced by environment, parental roles, religion, ethnicity, and knowledge.展开更多
This paper defines and explores the concept of social reproduction work,highlighting its significance and the fact that women are its primary contributors.It identifies key social issues faced by social reproduction w...This paper defines and explores the concept of social reproduction work,highlighting its significance and the fact that women are its primary contributors.It identifies key social issues faced by social reproduction workers,including marginalization,neglect,and unequal access to resources.Additionally,the study examines the primary challenges encountered by these workers:the lack of direct economic benefits,difficulty in balancing work and family responsibilities,and the significant physical and mental strain associated with unpaid labor.Using the theoretical framework of the capitalist economic system,the paper delves into the root causes of the caregiving crisis.Furthermore,it critiques the applicability of reproduction work theories in explaining unpaid care work in the Global South.Three key limitations are outlined:the neglect of informal economic systems in the Global South,persistent gender inequality,and the dynamic nature of global economic migration.展开更多
Sperm-structure-integrating nanodecorated microrobots have shown promise in medicine delivery and infertility treatment.A variety of spermbots use cutting-edge nanomaterials and 3D printing technology to enhance their...Sperm-structure-integrating nanodecorated microrobots have shown promise in medicine delivery and infertility treatment.A variety of spermbots use cutting-edge nanomaterials and 3D printing technology to enhance their functioning,such as biomimetic sperms and flagellate microorganisms.The success rates of assisted reproductive technology techniques like in vitro fertilisation(IVF)and intracytoplasmic sperm injection(ICSI)may increase as a result of these developments.Furthermore,the incorporation of artificial intelligence(AI)into spermbots has the potential to optimize reproductive therapies by reducing inherited illnesses through genetic screening and editing.However,before the widespread implementation of spermbots in clinical practice,several critical aspects must be addressed.Thorough investigations into biocompatibility,ethical considerations,and long-term safety are necessary to ensure that these technologies are safe and effective for in vivo applications.展开更多
[目的]雌牦牛性成熟晚是影响牦牛繁殖率的重要原因之一,分析不同发育时期牦牛卵巢的全基因组DNA甲基化差异,筛选出调控卵巢卵泡发育的差异甲基化基因,为研究表观遗传在牦牛卵巢组织生长发育过程中的作用提供数据基础。[方法]分别选取0.5...[目的]雌牦牛性成熟晚是影响牦牛繁殖率的重要原因之一,分析不同发育时期牦牛卵巢的全基因组DNA甲基化差异,筛选出调控卵巢卵泡发育的差异甲基化基因,为研究表观遗传在牦牛卵巢组织生长发育过程中的作用提供数据基础。[方法]分别选取0.5岁(幼年)、2.5岁(初情期)和4.5岁(性成熟)的牦牛各3头,采用全基因组重亚硫酸盐测序法,检测分析不同发育时期牦牛卵巢组织的全基因组DNA甲基化谱。[结果]牦牛卵巢组织中,甲基化位点以CpG序列类型为主,其甲基化水平明显高于CHG和CHH类型。CpG序列在CDS区、内含子和3′UTR的甲基化水平高于基因下游2 kb、基因上游2 kb和5′UTR。对比3个年龄组的甲基化位点,统计出3种类型的差异甲基化区域数量分别为4233(0.5 vs 2.5)、7302(2.5 vs 4.5)和1133(0.5 vs 4.5),其中CpG序列的差异甲基化区域分别对应334(0.5 vs 2.5)、640(2.5 vs 4.5)和371(0.5 vs 4.5)个差异甲基化基因。采用重亚硫酸盐测序法对测序结果进一步验证,结果显示变化趋势一致。GO和KEGG富集分析结果显示,CpG序列的差异甲基化基因显著富集到包括卵母细胞减数分裂、剪接体通路、Hippo信号通路、催产素信号通路、GnRH信号通路、Wnt信号通路、钙信号通路和Rap1信号通路等与卵巢卵泡发育相关的通路(P<0.05)。并从这些通路中筛选出8个甲基化差异显著且与牦牛卵巢卵泡发育相关的基因作为候选基因(P<0.05)。[结论]本研究利用全基因组重亚硫酸盐测序法检测分析了不同发育阶段牦牛卵巢全基因组甲基化谱,筛选出调控卵巢卵泡发育的8个候选基因,为进一步研究DNA甲基化对牦牛卵巢生长发育的调控机制提供数据基础。展开更多
文摘Background This study investigated the potential impacts of increasing linoleic andα-linolenic acid intake during lactation and wean-to-breeding on subsequent reproduction of sows.A total of 309 sows(PIC Camborough L42)were balanced by parity(140 and 169 sows representing parity 1 to 2[P1-2]and 3 to 9[P3+],respectively)and assigned within parity to a 2×2 factorial arrangement.Factors included essential fatty acid(EFA)supplementation(control diets containing 1.2%linoleic and 0.15%α-linolenic acid or diets with 3.0%linoleic and 0.38%α-linolenic acid)and supplementation period(lactation or wean-to-breeding).Tallow(low EFA diets)or soybean oil(high EFA diets)were included at 4%in sorghum-soybean meal-wheat middlings-based diets to attain targeted EFA levels.Results High levels of EFA fed during lactation had no effect on feed intake or litter performance,but increased subsequent farrowing rate(P=0.027;82.1%vs.70.4%),tended to reduce the proportion of sows removed(P=0.070;12.4%vs.20.8%),decreased the number of total pigs born in the following litter(P=0.072;15.3 vs.16.2),and increased total pigs born alive per 100 sows weaned(P=0.062;1,122 vs.974),regardless of sow parity.Young sows(P1-2)consuming the high EFA diet during lactation displayed a shorter wean-to-estrus interval(P=0.035;4.2 vs.4.6),but P3+sows were unaffected.Increasing EFA intake for P3+sows,but not P1-2 sows,resulted in more sows bred by d 5(P=0.028;91.1%vs.81.7%)and more mummies in the subsequent litter(P=0.040;0.32 vs.0.16).Feeding increased EFA to P1-2 sows during the wean-to-breeding period decreased subsequent farrowing rate(P=0.042;72.0%vs.87.7%),and increased removal rate(P=0.003;28.8%vs.9.4%).Total pigs born alive per 100 sows weaned was reduced(P=0.007)in P1-2 sows when supplemented with EFA during wean-breeding(939 vs.1,149)but was not impacted in P3+sows(1,131 vs.982).Conclusions Supplemental EFA in lactation diets benefited subsequent reproduction of sows,regardless of parity.Increasing dietary levels of EFA during the wean-to-breeding period to younger sows negatively impacted subsequent reproduction.
基金funded by a National Science Foundation award to Henning PM(no.2208975).
文摘Cytokinins are ancient hormones present across all kingdoms of life except archaea,although functional biosynthesis pathways have yet to be identified in animalia.Known for their roles in cell division and proliferation,cytokinins are critical to plant life,as they regulate various aspects of vegetative growth,stress response,and reproduction.In this review,we summarize literature from 2020 to 2025 pertaining to the cytokinin functions in plant reproduction.While general aspects of cytokinin’s role in plant reproduction have been addressed,we particularly focus on the role of cytokinin in reproductive systems due to recent work identifying their role as sex-determining factors in dioecious species in Salicaceae and other families,their role in determining flower sex in monoecious species,and their involvement in self-incompatibility response and asexual reproduction.
文摘Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadensis (L.) Nutt.) in the national economy. The trials were carried out in the experimental site of SamSU’s Botanic Garden, which is named after Sharof Rashidov. The study’s findings are based on the results obtained by planting cuttings of S. canadensis and E. macrophylla species of varying sizes and durations, as well as the cuttings’ roots and the impacts of growth chemicals.
基金Supported by the National Natural Science Foundation of China(No.32071528)。
文摘Environmental fluctuations are a pervasive natural phenomenon that typically favor diverse maternal investment strategies.Organisms across various evolutionary lineages have acquired the ability to enter the dormancy state to tolerate conditions that are unsuitable for normal life activities.In cyclical parthenogenesis,sexual reproduction is generally linked with dormancy,thereby enhancing the long-term fitness of populations through positive feedback mechanisms.This study evaluates the impact of environmental variability on offspring population dynamics through sexual reproduction in the rotifer Brachionus calyciflorus.We found that mictic females(sexual females)exposed to low-predictability environments produced resting eggs with a sporadic hatching pattern,reduced hatching rate,and smaller sizes of resting egg,embryo,and stem females,while increasing variations in fertilization among individuals to maximize population fitness.Variations in population growth could be manifested in enhanced reproductive capacity 2–3 d post-hatching,along with differences in fecundity among individuals.This suggests that dormant eggs from unstable environments may prioritize offspring survival and adjust to increased fertilization among individuals.This adaptive strategy enables rotifer populations to rapidly adjust to environmental changes,thus avoiding extinction and thriving in diverse habitats.Our systematic study enhances the understanding of how environmental stability affects long-term population fitness in cyclical parthenogens.
文摘In this paper,the author follows the trail of C.Malabou,Q.Meillassoux,and G.Deleuze and tries to test three philosophical concepts that seem to be particularly threatened in the era of automatic digital reproduction.These three concepts are plasticity(defended for many years by C.Malabou),contingency(reconstructed by Q.Meillassoux),and virtuality(developed by G.Deleuze).The main task of the text will be to reflect on which of these three concepts better protects our thinking against automation and stays faithful to the ideal of creativity.In what sense are plasticity,contingency,and the possibility of virtualization the a priori condition of any transformation,physical or intellectual,affective or conceptual metamorphosis?In what sense are these three concepts the only conditions for the survival of every living being?Would a being without contingency,plasticity,and disposition to virtualization simply be a dead being?
基金funded by Vietnam National University Ho Chi Minh City(VNU-HCM)under grant number NCM2020-28-01.
文摘Objective:To evaluate mobile applications available for patients undergoing assisted reproduction and assess the extent of their clinical validation.Methods:A systematic search was conducted in the Apple App Store and Google Play between September 1,2023 and September 30,2023 to identify mobile applications related to assisted reproduction.Apps were evaluated using the mobile app rating scale(MARS).In parallel,a literature search of PubMed,Scopus,Embase,and Web of Science was performed to identify clinical studies related to mobile applications in assisted reproduction.Clinical validation status and MARS scores were recorded,and findings were synthesized to highlight the gap between commercially available apps and research-based evidence.Results:From 1143 apps screened,11 met the inclusion criteria.Mean MARS score across apps was 3.63,with Leeaf scoring the highest(4.60).However,only one application(Embie)was supported by published research.The literature research identified 13 relevant studies,mostly randomized controlled trials,cohort studies,or usability studies.While research-based apps demonstrated clinical utility(e.g.,MediEmo,PreLiFe,Patient Journey App),most were unavailable on app stores.This revealed a disconnect between research-backed applications and those accessible to patients.Conclusions:Although several mobile apps for assisted reproduction demonstrate high usability and quality,few are clinically validated.The lack of integration between research and practice highlights the need for stronger collaboration between researchers,developers,and policymakers to ensure that patients access safe and effective tools.
基金Key R&D Plan of Liaoning Province(No.202000357-JH13/103):Construction of Liaoning Traditional Chinese Medicine Industry Technology Innovation Research InstituteNational Key Research and Development Plan Special Project(No.2019JH2/10300040)。
文摘The paper is an introduction to the front-end pulse acquisition and the back-end pulse biomimetic reproduction system.This system is capable of faithfully replicating the complete pulse waveform collected at the front end.Traditional Chinese Medicine(TCM)practitioners analyze and diagnose the pulse patterns at the replication end.Meanwhile,the obtained pulse waveforms are analyzed and learnt by a neural network based on key diagnostic points in TCM pulse taking,which enables the determination of the corresponding relationships between different pulse waveforms and various pulse patterns in TCM pulse taking.With the support of clinical samples,an auxiliary diagnostic system for TCM pulse patterns ensures the accuracy of pulse pattern replication.
基金supported by Kementerian RisetTeknologi dan Pendidikan Tinggi (Ministry of Research,Technology&Higher Education (RISTEKDIKTI) for funding International Collaboration Hibah Kerjasama International LP2M Universitas Jember 2021
文摘Objective: Adolescent reproduction health (ARH) differs greatly based on religions, religious norms, culture, and ethnicities in the Indonesian context. The value of local wisdom was considered too binding, traditional, and outdated, particularly on ARH in agricultural areas. The purpose of the study was to identify the problems, challenges, and needs, specifically for ARH, in the agricultural area of Indonesia. Methods: A qualitative study was performed among 41 communities, including 28 village officials (VOs), 9 community health volunteers (CHVs), and 9 adolescents. The data were collected through in-depth interviews and FGD. An thematic analysis was used to describe the phenomena. Results: This research obtained results regarding ARH in terms of communication patterns, daily behavior, reproductive practices, and hygiene of adolescent reproductive organs, especially in agricultural areas. Conclusions: The differences in the perspective of ARH are influenced by environment, parental roles, religion, ethnicity, and knowledge.
文摘This paper defines and explores the concept of social reproduction work,highlighting its significance and the fact that women are its primary contributors.It identifies key social issues faced by social reproduction workers,including marginalization,neglect,and unequal access to resources.Additionally,the study examines the primary challenges encountered by these workers:the lack of direct economic benefits,difficulty in balancing work and family responsibilities,and the significant physical and mental strain associated with unpaid labor.Using the theoretical framework of the capitalist economic system,the paper delves into the root causes of the caregiving crisis.Furthermore,it critiques the applicability of reproduction work theories in explaining unpaid care work in the Global South.Three key limitations are outlined:the neglect of informal economic systems in the Global South,persistent gender inequality,and the dynamic nature of global economic migration.
文摘Sperm-structure-integrating nanodecorated microrobots have shown promise in medicine delivery and infertility treatment.A variety of spermbots use cutting-edge nanomaterials and 3D printing technology to enhance their functioning,such as biomimetic sperms and flagellate microorganisms.The success rates of assisted reproductive technology techniques like in vitro fertilisation(IVF)and intracytoplasmic sperm injection(ICSI)may increase as a result of these developments.Furthermore,the incorporation of artificial intelligence(AI)into spermbots has the potential to optimize reproductive therapies by reducing inherited illnesses through genetic screening and editing.However,before the widespread implementation of spermbots in clinical practice,several critical aspects must be addressed.Thorough investigations into biocompatibility,ethical considerations,and long-term safety are necessary to ensure that these technologies are safe and effective for in vivo applications.
文摘[目的]雌牦牛性成熟晚是影响牦牛繁殖率的重要原因之一,分析不同发育时期牦牛卵巢的全基因组DNA甲基化差异,筛选出调控卵巢卵泡发育的差异甲基化基因,为研究表观遗传在牦牛卵巢组织生长发育过程中的作用提供数据基础。[方法]分别选取0.5岁(幼年)、2.5岁(初情期)和4.5岁(性成熟)的牦牛各3头,采用全基因组重亚硫酸盐测序法,检测分析不同发育时期牦牛卵巢组织的全基因组DNA甲基化谱。[结果]牦牛卵巢组织中,甲基化位点以CpG序列类型为主,其甲基化水平明显高于CHG和CHH类型。CpG序列在CDS区、内含子和3′UTR的甲基化水平高于基因下游2 kb、基因上游2 kb和5′UTR。对比3个年龄组的甲基化位点,统计出3种类型的差异甲基化区域数量分别为4233(0.5 vs 2.5)、7302(2.5 vs 4.5)和1133(0.5 vs 4.5),其中CpG序列的差异甲基化区域分别对应334(0.5 vs 2.5)、640(2.5 vs 4.5)和371(0.5 vs 4.5)个差异甲基化基因。采用重亚硫酸盐测序法对测序结果进一步验证,结果显示变化趋势一致。GO和KEGG富集分析结果显示,CpG序列的差异甲基化基因显著富集到包括卵母细胞减数分裂、剪接体通路、Hippo信号通路、催产素信号通路、GnRH信号通路、Wnt信号通路、钙信号通路和Rap1信号通路等与卵巢卵泡发育相关的通路(P<0.05)。并从这些通路中筛选出8个甲基化差异显著且与牦牛卵巢卵泡发育相关的基因作为候选基因(P<0.05)。[结论]本研究利用全基因组重亚硫酸盐测序法检测分析了不同发育阶段牦牛卵巢全基因组甲基化谱,筛选出调控卵巢卵泡发育的8个候选基因,为进一步研究DNA甲基化对牦牛卵巢生长发育的调控机制提供数据基础。
