The bottom aquaculture of yesso scallop (Patinopecten yessoensis) has developed rapidly in the north of Yellow Sea, China, but not without accompanying productivity problems. We, therefore, conducted field surveys to ...The bottom aquaculture of yesso scallop (Patinopecten yessoensis) has developed rapidly in the north of Yellow Sea, China, but not without accompanying productivity problems. We, therefore, conducted field surveys to investigate factors related to growth and mortality rates of bottom-cultured scallops after release. Specifically, we focused on the effects of total antioxidant capacity (TAOC) and three key enzymes involved in antioxidant and metabolic function:glutamic pyruvic transaminase (GPT), lactic dehydrogenase (LDH), and superoxide dismutase (SOD). Across summer and winter, we also measured the relative mRNA expression of GPT and SOD to understand their seasonal variation in the scallops, along with how such variation correlated to growth and mortality. Results show that bottom cultured scallops experienced mass mortality during the first six months post-release. During winter (December), scallops grew more rapidly and suffered less mortality than in summer (August). The observed lower performance probably resulted from less advantageous environmental factors during the summer seasons, such as high temperature and low dissolved oxygen. These environmental stressors enhance protein consumption while decreasing energetic resources in scallops. Furthermore, scallops in summer exhibited high antioxidant levels that probably competed for energy with process integral to growth and survival. These negative factors combined to elevate mortality rates. In conclusion, we provided evidence suggesting correlations between metabolic/antioxidant activity and growth and mortality of bottom-cultured yesso scallops. These correlations implied us an accurate method to estimate the performance of bottom culture system. Suggestions about innovative aquaculture techniques were also discussed in the study. Our results might provide a possible guideline to the improvement of bottom culture techniques for this commercially valuable seafood species.展开更多
试验旨在利用实时定量RT—PCR技术建立肉鸡组织钙结合蛋白（CaBP）基因相对表达量的测定方法．为进一步研究肉鸡钙吸收和利用的分子生物学机理以及骨骼的发育奠定技术基础。根据GenBank中鸡的CaBP基因序列，设计合成引物，进行SYBR Gre...试验旨在利用实时定量RT—PCR技术建立肉鸡组织钙结合蛋白（CaBP）基因相对表达量的测定方法．为进一步研究肉鸡钙吸收和利用的分子生物学机理以及骨骼的发育奠定技术基础。根据GenBank中鸡的CaBP基因序列，设计合成引物，进行SYBR Green Ⅰ实时定量RT—PCR。以管家基因B—Actin为内参基因，对组织Total RNA进行均一化处理，利用循环阈值（Ct值）的变化计算CaBP基因的相对表达量。结果表明，肉鸡十二指肠及胫骨组织CaBP基因相对表达量分别在2^7.78～2^-4.60和2^-2.46～2^-17.20范围。表明利用实时定量RT—PCR技术对肉鸡组织CaBP基因相对表达水平进行检测是可行的。展开更多
基金Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-48)the Taishan-Scholar Climbing Program of Shandong Provincethe Key Research Program of the Chinese Academy of Sciences(No.KFZD-SW-106)
文摘The bottom aquaculture of yesso scallop (Patinopecten yessoensis) has developed rapidly in the north of Yellow Sea, China, but not without accompanying productivity problems. We, therefore, conducted field surveys to investigate factors related to growth and mortality rates of bottom-cultured scallops after release. Specifically, we focused on the effects of total antioxidant capacity (TAOC) and three key enzymes involved in antioxidant and metabolic function:glutamic pyruvic transaminase (GPT), lactic dehydrogenase (LDH), and superoxide dismutase (SOD). Across summer and winter, we also measured the relative mRNA expression of GPT and SOD to understand their seasonal variation in the scallops, along with how such variation correlated to growth and mortality. Results show that bottom cultured scallops experienced mass mortality during the first six months post-release. During winter (December), scallops grew more rapidly and suffered less mortality than in summer (August). The observed lower performance probably resulted from less advantageous environmental factors during the summer seasons, such as high temperature and low dissolved oxygen. These environmental stressors enhance protein consumption while decreasing energetic resources in scallops. Furthermore, scallops in summer exhibited high antioxidant levels that probably competed for energy with process integral to growth and survival. These negative factors combined to elevate mortality rates. In conclusion, we provided evidence suggesting correlations between metabolic/antioxidant activity and growth and mortality of bottom-cultured yesso scallops. These correlations implied us an accurate method to estimate the performance of bottom culture system. Suggestions about innovative aquaculture techniques were also discussed in the study. Our results might provide a possible guideline to the improvement of bottom culture techniques for this commercially valuable seafood species.
文摘试验旨在利用实时定量RT—PCR技术建立肉鸡组织钙结合蛋白（CaBP）基因相对表达量的测定方法．为进一步研究肉鸡钙吸收和利用的分子生物学机理以及骨骼的发育奠定技术基础。根据GenBank中鸡的CaBP基因序列，设计合成引物，进行SYBR Green Ⅰ实时定量RT—PCR。以管家基因B—Actin为内参基因，对组织Total RNA进行均一化处理，利用循环阈值（Ct值）的变化计算CaBP基因的相对表达量。结果表明，肉鸡十二指肠及胫骨组织CaBP基因相对表达量分别在2^7.78～2^-4.60和2^-2.46～2^-17.20范围。表明利用实时定量RT—PCR技术对肉鸡组织CaBP基因相对表达水平进行检测是可行的。
