[Objective]The paper was to establish a rapid identification method of Bactrocera cilifera(Hendel)with species-specific primers(SS-COI).[Method]Using B.cilifera(Hendel)as the positive control,and 19 species of fruit f...[Objective]The paper was to establish a rapid identification method of Bactrocera cilifera(Hendel)with species-specific primers(SS-COI).[Method]Using B.cilifera(Hendel)as the positive control,and 19 species of fruit flies such as B.diaphora(Coquillett)and B.dorsalis(Hendel)as the negative controls,a pair of species-specific primers,YF290 and YR511,were designed and screened for accurate identification of B.cilifera,based on mitochondrial DNA COI sequence.[Result]The PCR products were amplified and detected by electrophoresis.Only a clear and single band was observed at about 222 bp in the positive control,while no bands were found in the other negative controls.[Conclusion]The established rapid identification method with species-specific primers(SS-COI)is of great practical significance for rapid identification of fruit flies intercepted from import and export fruits and vegetables at ports,and for rapid clearance and early warning of import fruits and vegetables at ports.展开更多
Objective To construct a rapid and high-throughput assay for identifying recombinant bacteria based on mass spectrometry. Methods Matrix assisted laser desorption ionization time-of-flight mass spectrometry （MALDI-TO...Objective To construct a rapid and high-throughput assay for identifying recombinant bacteria based on mass spectrometry. Methods Matrix assisted laser desorption ionization time-of-flight mass spectrometry （MALDI-TOF MS） techniques were used to identify 12 recombinant proteins （10 of Yersinia pestis, 1 of Campylobacter jejuni and 1 of Helicobacter pylori）. A classification model for the various phase of recombinant bacteria was established, optimized and validated, using MALDI-TOF MS-CIinProTools system. The differences in the peptide mass spectra were analyzed by using Biotyper and FIexAnalysis softwares. Results Models of GA, SNN, and QC were established. After optimizing the parameters, the GA recognition model showed good classification capabilities： RC=100%, mean CVA=98.7% （the CVA was 96.4% in phase 1, 100% in phase 2, 98.4% in phase 3, and 100% in phase 4, respectively） and PPV=95}. This model can be used to classify the bacteria and their recombinant, which only requires 3.7x103 cells for analysis. The total time needed is only 10 min from protein extraction to reporting the result for one sample. Furthermore, this assay can automatically detect and test 96 samples concurrently. A total of 48 specific peaks （9, 16, 9, and 14 for the four stages, respectively） was found in the various phase of recombinant bacteria. Conclusion MALDI-TOF MS can be used as a fast, accurate, and high-throughput method to identify recombinant bacteria, which provide a new ideas not only for recombinant bacteria but also for the identification of mutant strains and bioterrorism pathogens.展开更多
Switched systems, i.e., systems changing the parameter values(even structural forms) abruptly and randomly at arbitrary instants, have been extensively utilized in many fields of modern industries. Rapid identificatio...Switched systems, i.e., systems changing the parameter values(even structural forms) abruptly and randomly at arbitrary instants, have been extensively utilized in many fields of modern industries. Rapid identification of switched systems, i.e.,capturing all the changing instants and reconstructing the mathematical models rapidly, is of great significance for behavior prediction, performance evaluation and possible control, but is restricted by small data amount available. Here, the rapid identification problem is successfully solved by a data-driven method in variational framework. The data-driven method only requires a small amount of data due to the compact form of the variational description, and is robust to data noise due to the holistic viewpoint. Two numerical examples, i.e., Duffing oscillator and van der Pol system(as two representative systems in nonlinear dynamics), are adopted to illustrate its application, efficiency and robustness to noise.展开更多
There is an urgent need for the development of a method that can undertake rapid, effective, and accurate monitoring and identification of fog by satellite remote sensing, since heavy fog can cause enormous disasters ...There is an urgent need for the development of a method that can undertake rapid, effective, and accurate monitoring and identification of fog by satellite remote sensing, since heavy fog can cause enormous disasters to China’s national economy and people's lives and property in the urban and coastal areas. In this paper, the correlative relationship between the reflectivity of land surface and clouds in different time phases is found, based on the analysis of the radiative and satellite-based spectral characteristics of fog. Through calculation and analyses of the relative variability of the reflectivity in the images, the threshold to identify quasi-fog areas is generated automatically. Furthermore, using the technique of quick image run-length encoding, and in combination with such practical methods as analyzing texture and shape fractures, smoothness, and template characteristics, the automatic identification of fog and fog-cloud separation using meteorological satellite remote sensing images are studied, with good results in application.展开更多
The rapid PCR identification technology plays a crucial role in the quality control of traditional Chinese medicinal materials and decoction pieces.This technology,based on the principle of polymerase chain reaction(P...The rapid PCR identification technology plays a crucial role in the quality control of traditional Chinese medicinal materials and decoction pieces.This technology,based on the principle of polymerase chain reaction(PCR),can quickly and accurately identify the authenticity of medicinal materials,thereby ensuring the safety and effectiveness of drugs.To promote the standardized development of rapid PCR identification,the China Association of Chinese Medicine has approved and issued 3 group standards led by the National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences.These standards are Quickly PCR Identification of Lonicerae japonicae flos(T/CACM 013-2016),Quickly PCR Identification of Bungarus parvus(T/CACM 012-2016),and Quickly PCR Identification of Rhizoma bletillae(T/CACM 1027.105-2018).This paper provides a detailed interpretation of the above standards,expounds on the principles,methods,and applications of rapid PCR identification,and its significance in the traditional Chinese medicine industry,so as to promote the development of rapid PCR identification and the inheritance and innovation of traditional Chinese medicine identification.展开更多
Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by s...Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.展开更多
During the COVID-19 pandemic,hospital wards served as crucial medical infrastructure for patient treatment.The existing ventilation systems in hospital wards are often criticized for their high energy consumption and ...During the COVID-19 pandemic,hospital wards served as crucial medical infrastructure for patient treatment.The existing ventilation systems in hospital wards are often criticized for their high energy consumption and poor pollutant removal.This study introduces an improved active chilled beam(ACB)system for wards.This system integrates an ionization module with the ACB,utilizing the ACB’s supply air jet and exhaust air convergence to create a directed airflow within the ward,effectively purifying aerosols.First,a validated CFD model is employed to simulate airflow generated by the ACB and the movement of aerosols exhaled by patients.Particle image velocimetry(PIV)is then used to visualize the indoor directed airflow created by the ACB.The targeted aerosol removal performance of the ACB system is quantitatively described using the TARGETING index.To address the cumbersome calculation of the TARGETING index,a qualitative rapid identification method for targeted ventilation is proposed.Finally,the purification effect of the ACB system in multipatient wards was optimized through theoretical analysis.Compared with existing systems,the improved ACB system enhances the targeted aerosol removal performance by 41.9%–62.5%,and achieves energy savings of 33.3%.In multipatient wards,the ACB system can reduce the cross-infection risk by 80.5%–91.0%.展开更多
基金Supported by Natural Science Foundation of Fujian Province (2011J01066, 2012JO1061)。
文摘[Objective]The paper was to establish a rapid identification method of Bactrocera cilifera(Hendel)with species-specific primers(SS-COI).[Method]Using B.cilifera(Hendel)as the positive control,and 19 species of fruit flies such as B.diaphora(Coquillett)and B.dorsalis(Hendel)as the negative controls,a pair of species-specific primers,YF290 and YR511,were designed and screened for accurate identification of B.cilifera,based on mitochondrial DNA COI sequence.[Result]The PCR products were amplified and detected by electrophoresis.Only a clear and single band was observed at about 222 bp in the positive control,while no bands were found in the other negative controls.[Conclusion]The established rapid identification method with species-specific primers(SS-COI)is of great practical significance for rapid identification of fruit flies intercepted from import and export fruits and vegetables at ports,and for rapid clearance and early warning of import fruits and vegetables at ports.
基金supported by the National Key Program for Infectious Disease of China(Contract No.2013ZX10004216-002)Key Projects in the National Science&Technology Pillar Program during the 12th Five-year Plan Period(Contract No.2012BAI06B02)the Science and Technology Program of Zhejiang Province Public Technology Social Development Project(No.2010C33035)
文摘Objective To construct a rapid and high-throughput assay for identifying recombinant bacteria based on mass spectrometry. Methods Matrix assisted laser desorption ionization time-of-flight mass spectrometry （MALDI-TOF MS） techniques were used to identify 12 recombinant proteins （10 of Yersinia pestis, 1 of Campylobacter jejuni and 1 of Helicobacter pylori）. A classification model for the various phase of recombinant bacteria was established, optimized and validated, using MALDI-TOF MS-CIinProTools system. The differences in the peptide mass spectra were analyzed by using Biotyper and FIexAnalysis softwares. Results Models of GA, SNN, and QC were established. After optimizing the parameters, the GA recognition model showed good classification capabilities： RC=100%, mean CVA=98.7% （the CVA was 96.4% in phase 1, 100% in phase 2, 98.4% in phase 3, and 100% in phase 4, respectively） and PPV=95}. This model can be used to classify the bacteria and their recombinant, which only requires 3.7x103 cells for analysis. The total time needed is only 10 min from protein extraction to reporting the result for one sample. Furthermore, this assay can automatically detect and test 96 samples concurrently. A total of 48 specific peaks （9, 16, 9, and 14 for the four stages, respectively） was found in the various phase of recombinant bacteria. Conclusion MALDI-TOF MS can be used as a fast, accurate, and high-throughput method to identify recombinant bacteria, which provide a new ideas not only for recombinant bacteria but also for the identification of mutant strains and bioterrorism pathogens.
基金supported by the National Natural Science Foundation of China (Grant Nos. 11872328 and 11472240)supported by the National Natural Science Foundation of China (Grant Nos.11532011 and 11621062)。
文摘Switched systems, i.e., systems changing the parameter values(even structural forms) abruptly and randomly at arbitrary instants, have been extensively utilized in many fields of modern industries. Rapid identification of switched systems, i.e.,capturing all the changing instants and reconstructing the mathematical models rapidly, is of great significance for behavior prediction, performance evaluation and possible control, but is restricted by small data amount available. Here, the rapid identification problem is successfully solved by a data-driven method in variational framework. The data-driven method only requires a small amount of data due to the compact form of the variational description, and is robust to data noise due to the holistic viewpoint. Two numerical examples, i.e., Duffing oscillator and van der Pol system(as two representative systems in nonlinear dynamics), are adopted to illustrate its application, efficiency and robustness to noise.
基金Key research project "Research of Shanghai City and Costal Heavy Fog Remote Sensing Detecting and Warning System" of Science and Technology Commission of Shanghai Municipality (075115011)
文摘There is an urgent need for the development of a method that can undertake rapid, effective, and accurate monitoring and identification of fog by satellite remote sensing, since heavy fog can cause enormous disasters to China’s national economy and people's lives and property in the urban and coastal areas. In this paper, the correlative relationship between the reflectivity of land surface and clouds in different time phases is found, based on the analysis of the radiative and satellite-based spectral characteristics of fog. Through calculation and analyses of the relative variability of the reflectivity in the images, the threshold to identify quasi-fog areas is generated automatically. Furthermore, using the technique of quick image run-length encoding, and in combination with such practical methods as analyzing texture and shape fractures, smoothness, and template characteristics, the automatic identification of fog and fog-cloud separation using meteorological satellite remote sensing images are studied, with good results in application.
基金financially supported by the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CI2023D001)the National Science Fund for Distinguished Young Scholars(No.82325049).
文摘The rapid PCR identification technology plays a crucial role in the quality control of traditional Chinese medicinal materials and decoction pieces.This technology,based on the principle of polymerase chain reaction(PCR),can quickly and accurately identify the authenticity of medicinal materials,thereby ensuring the safety and effectiveness of drugs.To promote the standardized development of rapid PCR identification,the China Association of Chinese Medicine has approved and issued 3 group standards led by the National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences.These standards are Quickly PCR Identification of Lonicerae japonicae flos(T/CACM 013-2016),Quickly PCR Identification of Bungarus parvus(T/CACM 012-2016),and Quickly PCR Identification of Rhizoma bletillae(T/CACM 1027.105-2018).This paper provides a detailed interpretation of the above standards,expounds on the principles,methods,and applications of rapid PCR identification,and its significance in the traditional Chinese medicine industry,so as to promote the development of rapid PCR identification and the inheritance and innovation of traditional Chinese medicine identification.
基金supported by the National Transgenic Major Program of China(No.2019ZX08010-002)the China Agriculture Research System(No.CARS-21)the Major Science and Technology Projects of Breeding New Varieties of Agriculture in Zhejiang Province,China(No.2016C02058)。
文摘Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.
基金the National Key R&D Program from the Ministry of Science and Technology,China,on“Comprehensive control of air,soil and groundwater pollution”through Grant No.2022YFC3702803the National Natural Science Foundation of China(NSFC)through grant No.52108084.
文摘During the COVID-19 pandemic,hospital wards served as crucial medical infrastructure for patient treatment.The existing ventilation systems in hospital wards are often criticized for their high energy consumption and poor pollutant removal.This study introduces an improved active chilled beam(ACB)system for wards.This system integrates an ionization module with the ACB,utilizing the ACB’s supply air jet and exhaust air convergence to create a directed airflow within the ward,effectively purifying aerosols.First,a validated CFD model is employed to simulate airflow generated by the ACB and the movement of aerosols exhaled by patients.Particle image velocimetry(PIV)is then used to visualize the indoor directed airflow created by the ACB.The targeted aerosol removal performance of the ACB system is quantitatively described using the TARGETING index.To address the cumbersome calculation of the TARGETING index,a qualitative rapid identification method for targeted ventilation is proposed.Finally,the purification effect of the ACB system in multipatient wards was optimized through theoretical analysis.Compared with existing systems,the improved ACB system enhances the targeted aerosol removal performance by 41.9%–62.5%,and achieves energy savings of 33.3%.In multipatient wards,the ACB system can reduce the cross-infection risk by 80.5%–91.0%.
