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Random walk modeling of wake dispersion for the exhaust tower of an underground tunnel in urban area 被引量:2
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作者 JIANG Wei\|mei\+1, YU Hong\|bin\+1, LI Xin\+2 (1.Department of Atmospheric Sciences, Nanjing University, Nanjing 210093, China 2.LAPC, Institute of Atmospheric Physics,Chinese Academy of Sciences, Beijing 100029, China) 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 1999年第4期474-479,共6页
In this paper, some experimental studies on the impact of effluent from an exhaust tower of an underground tunnel with special construction are reported. By measuring the flow field downstream of the tower in NJU mete... In this paper, some experimental studies on the impact of effluent from an exhaust tower of an underground tunnel with special construction are reported. By measuring the flow field downstream of the tower in NJU meteorological wind tunnel, some flow characteristics in the make area were established. Based on these, an advanced random\|walk dispersion model was set up and applied successfully to the simulation of dispersion in the wake area. The modelling results were in accordance with wind tunnel measurements. The computed maximum of ground surface concentration in the building case was a factor of 3-4 higher than that in the flat case and appeared much closer to the source. The simulation indicated that random walk modelling is an effective and practical tool for the wake stream impact assessment. 展开更多
关键词 exhaust tower air pollution in urban area atmospheric dispersion random walk modelling
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Modeling random telegraph signal noise in CMOS image sensor under low light based on binomial distribution 被引量:2
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作者 张钰 逯鑫淼 +2 位作者 王光义 胡永才 徐江涛 《Chinese Physics B》 SCIE EI CAS CSCD 2016年第7期164-170,共7页
The random telegraph signal noise in the pixel source follower MOSFET is the principle component of the noise in the CMOS image sensor under low light. In this paper, the physical and statistical model of the random t... The random telegraph signal noise in the pixel source follower MOSFET is the principle component of the noise in the CMOS image sensor under low light. In this paper, the physical and statistical model of the random telegraph signal noise in the pixel source follower based on the binomial distribution is set up. The number of electrons captured or released by the oxide traps in the unit time is described as the random variables which obey the binomial distribution. As a result,the output states and the corresponding probabilities of the first and the second samples of the correlated double sampling circuit are acquired. The standard deviation of the output states after the correlated double sampling circuit can be obtained accordingly. In the simulation section, one hundred thousand samples of the source follower MOSFET have been simulated,and the simulation results show that the proposed model has the similar statistical characteristics with the existing models under the effect of the channel length and the density of the oxide trap. Moreover, the noise histogram of the proposed model has been evaluated at different environmental temperatures. 展开更多
关键词 random telegraph signal noise physical and statistical model binomial distribution CMOS image sensor
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Statistical Modeling of Gate Capacitance Variations Induced by Random Dopants in Nanometer MOSFETs Reserving Correlations
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作者 吕伟锋 王光义 +1 位作者 林弥 孙玲玲 《Chinese Physics Letters》 SCIE CAS CSCD 2015年第10期159-161,共3页
We consider intrinsic gate capacitance variations due to random dopants in the nanometer metal oxide semi- conductor field effect transistor (MOSFET) channel. The variations of total gate capacitance and gate transc... We consider intrinsic gate capacitance variations due to random dopants in the nanometer metal oxide semi- conductor field effect transistor (MOSFET) channel. The variations of total gate capacitance and gate transcapacitances are investigated and the strong correlations between the trans-capacitance variations are discovered. A simple statistical model is proposed for accurately capturing total gate capacitance variability based on the correlations. The model fits very well with the Monte Carlo simulations and the average errors are -0.033% for n-type metal-oxide semiconductor and -0.012% for p-type metal-oxide semiconductor, respectively. Our simulation studies also indicate that, owing to these correlations, the total gate capacitance variability will not dominate in gate capacitance variations. 展开更多
关键词 MOSFET Statistical modeling of Gate Capacitance Variations Induced by random Dopants in Nanometer MOSFETs Reserving Correlations
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Random walk dispersion model for missile contrail particles in cross-airspace environments
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作者 Chenshuo Li Debin Fu Tianyu Wei 《Defence Technology(防务技术)》 2025年第7期307-320,共14页
Missiles provide long-range precision strike capabilities and have become a cornerstone of modern warfare.The contrail clouds formed by missile during their active flight phase present significant chal-lenges to high-... Missiles provide long-range precision strike capabilities and have become a cornerstone of modern warfare.The contrail clouds formed by missile during their active flight phase present significant chal-lenges to high-altitude environmental observation and target detection and tracking.Existing studies primarily focus on specific airspace regions,leaving critical gaps in understanding the effects of long dispersion times,wide altitude ranges,and variable atmospheric conditions on missile contrail clouds.To address these gaps,this article develops a numerical method based on the Lagrangian random walk model,which incorporates various velocity variation terms,including particle velocity caused by the difference of wind field,by the thermal motion of local gas molecules and by random collisions between contrail cloud particles to capture the influence of environmental wind fields,atmospheric conditions,and particle concentrations on the motion of contrail cloud particles.A general coordinate system aligned with the missile's flight trajectory is employed to represent particle distribution characteristics.The proposed method is in good agreement with the conducted experiments as well as with the available numerical simulations.The results demonstrate that the proposed model effectively simulates the dispersion state of contrail clouds,accurately reflecting the impact of large-scale wind field variations and altitude changes with high computational efficiency.Additionally,simulation results indicate that the increased distance between gas molecules in rarefied environments facilitates enhanced particle dispersion,while larger particles exhibit a faster dispersion rate due to their greater mass. 展开更多
关键词 Missile contrail Dispersion process random walk model Concentration distribution Cross-airspace
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AI-Driven Malware Detection with VGG Feature Extraction and Artificial Rabbits Optimized Random Forest Model
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作者 Brij B.Gupta Akshat Gaurav +3 位作者 Wadee Alhalabi Varsha Arya Shavi Bansal Ching-Hsien Hsu 《Computers, Materials & Continua》 2025年第9期4755-4772,共18页
Detecting cyber attacks in networks connected to the Internet of Things(IoT)is of utmost importance because of the growing vulnerabilities in the smart environment.Conventional models,such as Naive Bayes and support v... Detecting cyber attacks in networks connected to the Internet of Things(IoT)is of utmost importance because of the growing vulnerabilities in the smart environment.Conventional models,such as Naive Bayes and support vector machine(SVM),as well as ensemble methods,such as Gradient Boosting and eXtreme gradient boosting(XGBoost),are often plagued by high computational costs,which makes it challenging for them to perform real-time detection.In this regard,we suggested an attack detection approach that integrates Visual Geometry Group 16(VGG16),Artificial Rabbits Optimizer(ARO),and Random Forest Model to increase detection accuracy and operational efficiency in Internet of Things(IoT)networks.In the suggested model,the extraction of features from malware pictures was accomplished with the help of VGG16.The prediction process is carried out by the random forest model using the extracted features from the VGG16.Additionally,ARO is used to improve the hyper-parameters of the random forest model of the random forest.With an accuracy of 96.36%,the suggested model outperforms the standard models in terms of accuracy,F1-score,precision,and recall.The comparative research highlights our strategy’s success,which improves performance while maintaining a lower computational cost.This method is ideal for real-time applications,but it is effective. 展开更多
关键词 Malware detection VGG feature extraction artificial rabbits OPTIMIZATION random forest model
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A non-affine constitutive model for the extremely large deformation of hydrogel polymer network based on network modeling method
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作者 Jincheng Lei Yuan Gao +1 位作者 Danyang Wang Zishun Liu 《Acta Mechanica Sinica》 2025年第7期69-80,共12页
Current hyperelastic constitutive models of hydrogels face difficulties in capturing the stress-strain behaviors of hydrogels under extremely large deformation because the effect of non-affine deformation of the polym... Current hyperelastic constitutive models of hydrogels face difficulties in capturing the stress-strain behaviors of hydrogels under extremely large deformation because the effect of non-affine deformation of the polymer network inside is ambiguous.In this work,we construct periodic random network(PRN)models for the effective polymer network in hydrogels and investigate the non-affine deformation of polymer chains intrinsically originates from the structural randomness from bottom up.The non-affine deformation in PRN models is manifested as the actual stretch of polymer chains randomly deviated from the chain stretch predicted by affine assumption,and quantified by a non-affine ratio of each polymer chain.It is found that the non-affine ratios of polymer chains are closely related to bulk deformation state,chain orientation,and initial chain elongation.By fitting the non-affine ratio of polymer chains in all PRN models,we propose a non-affine constitutive model for the hydrogel polymer network based on micro-sphere model.The stress-strain curves of the proposed constitutive models under uniaxial tension condition agree with the simulation results of different PRN models of hydrogels very well. 展开更多
关键词 Non-affine deformation Periodic random network model Large deformation Constitutive model
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A Hybrid Air Quality Prediction Method Based on VAR and Random Forest
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作者 Minghao Yi Fuming Lin 《Journal of Computer and Communications》 2025年第2期142-154,共13页
To improve the efficiency of air quality analysis and the accuracy of predictions, this paper proposes a composite method based on Vector Autoregressive (VAR) and Random Forest (RF) models. In the theoretical section,... To improve the efficiency of air quality analysis and the accuracy of predictions, this paper proposes a composite method based on Vector Autoregressive (VAR) and Random Forest (RF) models. In the theoretical section, the model introduction and estimation algorithms are provided. In the empirical analysis section, global air quality data from 2022 to 2024 are used, and the proposed method is applied. Specifically, principal component analysis (PCA) is first conducted, and then VAR and Random Forest methods are used for prediction on the reduced-dimensional data. The results show that the RMSE of the hybrid model is 45.27, significantly lower than the 49.11 of the VAR model alone, verifying its superiority. The stability and predictive performance of the model are effectively enhanced. 展开更多
关键词 Var Model Principal Component Analysis random Forest Model
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DUAL RANDOM MODEL OF INCREASING ANNUITY 被引量:6
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作者 He Wenjiong Zhang YiEconomic College & Science College, Zhejiang Univ.(Xixi Campus),Hangzhou 310028. 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2001年第4期430-438,共9页
The dual random models about the life insurance and social pension insurance have received considerable attention in the recent articles on actuarial theory and applications. This paper discusses a general kind of inc... The dual random models about the life insurance and social pension insurance have received considerable attention in the recent articles on actuarial theory and applications. This paper discusses a general kind of increasing annuity based on its force of interest accumulation function as a general random process. The dual random model of the present value of the benefits of the increasing annuity has been set, and their moments have been calculated under certain conditions. 展开更多
关键词 Increasing annuity random model independent increment process.
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EMPIRICAL BAYES TEST PROBLEMS OF VARIANCE COMPONENTS IN RANDOM EFFECTS MODEL 被引量:3
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作者 韦来生 张伟平 《Acta Mathematica Scientia》 SCIE CSCD 2005年第2期274-282,共9页
Bayes decision rule of variance components for one-way random effects model is derived and empirical Bayes (EB) decision rules are constructed by kernel estimation method. Under suitable conditions, it is shown that t... Bayes decision rule of variance components for one-way random effects model is derived and empirical Bayes (EB) decision rules are constructed by kernel estimation method. Under suitable conditions, it is shown that the proposed EB decision rules are asymptotically optimal with convergence rates near O(n-1/2). Finally, an example concerning the main result is given. 展开更多
关键词 Empirical Bayes test variance components random effects model convergence rates
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A LAW OF ITERATED LOGARITHM FOR THE MLE IN A RANDOM CENSORING MODEL WITH INCOMPLETE INFORMATION 被引量:2
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作者 宋凤丽 刘禄勤 《Acta Mathematica Scientia》 SCIE CSCD 2008年第3期501-512,共12页
In this article, a law of iterated logarithm for the maximum likelihood estimator in a random censoring model with incomplete information under certain regular conditions is obtained.
关键词 random censoring model maximum likelihood estimator law of iterated logarithm
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OUTLIER TEST IN RANDOMIZED LINEAR MODEL 被引量:2
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作者 XIANGLIMING SHILEI 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 1994年第1期65-75,共11页
In this papert we give an approach for detecting one or more outliers inrandomized linear model.The likelihood ratio test statistic and its distributions underthe null hypothesis and the alternative hypothesis are giv... In this papert we give an approach for detecting one or more outliers inrandomized linear model.The likelihood ratio test statistic and its distributions underthe null hypothesis and the alternative hypothesis are given. Furthermore,the robustnessof the test statistic in a certain sense is proved. Finally,the optimality properties of thetest are derived. 展开更多
关键词 randomized Linear Model.Outliers Likelihood Ratio Test UNIFORMLY
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Desertification status mapping in MuttumaWatershed by using Random Forest Model 被引量:1
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作者 S.Dharumarajan Thomas F.A.Bishop 《Research in Cold and Arid Regions》 CSCD 2022年第1期32-42,共11页
Potential of the Random Forest Model on mapping of different desertification processes was studied in Muttuma watershed of mid-Murrumbidgee river region of New South Wales,Australia.Desertification vulnerability index... Potential of the Random Forest Model on mapping of different desertification processes was studied in Muttuma watershed of mid-Murrumbidgee river region of New South Wales,Australia.Desertification vulnerability index was developed using climate,terrain,vegetation,soil and land quality indices to identify environmentally sensitive areas for desertification.Random Forest Model(RFM)was used to predict the different desertification processes such as soil erosion,salinization and waterlogging in the watershed and the information needed to train classification algorithms was obtained from satellite imagery interpretation and ground truth data.Climatic factors(evaporation,rainfall,temperature),terrain factors(aspect,slope,slope length,steepness,and wetness index),soil properties(pH,organic carbon,clay and sand content)and vulnerability indices were used as an explanatory variable.Classification accuracy and kappa index were calculated for training and testing datasets.We recorded an overall accuracy rate of 87.7%and 72.1%for training and testing sites,respectively.We found larger discrepancies between overall accuracy rate and kappa index for testing datasets(72.2%and 27.5%,respectively)suggesting that all the classes are not predicted well.The prediction of soil erosion and no desertification process was good and poor for salinization and water-logging process.Overall,the results observed give a new idea of using the knowledge of desertification process in training areas that can be used to predict the desertification processes at unvisited areas. 展开更多
关键词 desertification processes vulnerability indices random Forest Model EXTRAPOLATION
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Magnetic-resonance image segmentation based on improved variable weight multi-resolution Markov random field in undecimated complex wavelet domain 被引量:1
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作者 Hong Fan Yiman Sun +3 位作者 Xiaojuan Zhang Chengcheng Zhang Xiangjun Li Yi Wang 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第7期655-667,共13页
To solve the problem that the magnetic resonance(MR)image has weak boundaries,large amount of information,and low signal-to-noise ratio,we propose an image segmentation method based on the multi-resolution Markov rand... To solve the problem that the magnetic resonance(MR)image has weak boundaries,large amount of information,and low signal-to-noise ratio,we propose an image segmentation method based on the multi-resolution Markov random field(MRMRF)model.The algorithm uses undecimated dual-tree complex wavelet transformation to transform the image into multiple scales.The transformed low-frequency scale histogram is used to improve the initial clustering center of the K-means algorithm,and then other cluster centers are selected according to the maximum distance rule to obtain the coarse-scale segmentation.The results are then segmented by the improved MRMRF model.In order to solve the problem of fuzzy edge segmentation caused by the gray level inhomogeneity of MR image segmentation under the MRMRF model,it is proposed to introduce variable weight parameters in the segmentation process of each scale.Furthermore,the final segmentation results are optimized.We name this algorithm the variable-weight multi-resolution Markov random field(VWMRMRF).The simulation and clinical MR image segmentation verification show that the VWMRMRF algorithm has high segmentation accuracy and robustness,and can accurately and stably achieve low signal-to-noise ratio,weak boundary MR image segmentation. 展开更多
关键词 undecimated dual-tree complex wavelet MR image segmentation multi-resolution Markov random field model
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Remaining useful life prediction based on nonlinear random coefficient regression model with fusing failure time data 被引量:4
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作者 WANG Fengfei TANG Shengjin +3 位作者 SUN Xiaoyan LI Liang YU Chuanqiang SI Xiaosheng 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2023年第1期247-258,共12页
Remaining useful life(RUL) prediction is one of the most crucial elements in prognostics and health management(PHM). Aiming at the imperfect prior information, this paper proposes an RUL prediction method based on a n... Remaining useful life(RUL) prediction is one of the most crucial elements in prognostics and health management(PHM). Aiming at the imperfect prior information, this paper proposes an RUL prediction method based on a nonlinear random coefficient regression(RCR) model with fusing failure time data.Firstly, some interesting natures of parameters estimation based on the nonlinear RCR model are given. Based on these natures,the failure time data can be fused as the prior information reasonably. Specifically, the fixed parameters are calculated by the field degradation data of the evaluated equipment and the prior information of random coefficient is estimated with fusing the failure time data of congeneric equipment. Then, the prior information of the random coefficient is updated online under the Bayesian framework, the probability density function(PDF) of the RUL with considering the limitation of the failure threshold is performed. Finally, two case studies are used for experimental verification. Compared with the traditional Bayesian method, the proposed method can effectively reduce the influence of imperfect prior information and improve the accuracy of RUL prediction. 展开更多
关键词 remaining useful life(RUL)prediction imperfect prior information failure time data NONLINEAR random coefficient regression(RCR)model
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Error Threshold of Fully Random Eigen Model 被引量:1
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作者 李多芳 曹天光 +3 位作者 耿金鹏 乔丽华 顾建中 展永 《Chinese Physics Letters》 SCIE CAS CSCD 2015年第1期170-173,共4页
Species evolution is essentially a random process of interaction between biological populations and their environ- ments. As a result, some physical parameters in evolution models are subject to statistical fluctuatio... Species evolution is essentially a random process of interaction between biological populations and their environ- ments. As a result, some physical parameters in evolution models are subject to statistical fluctuations. In this work, two important parameters in the Eigen model, the fitness and mutation rate, are treated as Gaassian dis- tributed random variables simultaneously to examine the property of the error threshold. Numerical simulation results show that the error threshold in the fully random model appears as a crossover region instead of a phase transition point, and &s the fluctuation strength increases the crossover region becomes smoother and smoother. Furthermore, it is shown that the randomization of the mutation rate plays a dominant role in changing the error threshold in the fully random model, which is consistent with the existing experimental data. The implication of the threshold change due to the randomization for antiviral strategies is discussed. 展开更多
关键词 Error Threshold of Fully random Eigen Model
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An Image Segmentation Algorithm Based on a Local Region Conditional Random Field Model 被引量:1
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作者 Xiao Jiang Haibin Yu Shuaishuai Lv 《International Journal of Communications, Network and System Sciences》 2020年第9期139-159,共21页
To reduce the computation cost of a combined probabilistic graphical model and a deep neural network in semantic segmentation, the local region condition random field (LRCRF) model is investigated which selectively ap... To reduce the computation cost of a combined probabilistic graphical model and a deep neural network in semantic segmentation, the local region condition random field (LRCRF) model is investigated which selectively applies the condition random field (CRF) to the most active region in the image. The full convolutional network structure is optimized with the ResNet-18 structure and dilated convolution to expand the receptive field. The tracking networks are also improved based on SiameseFC by considering the frame relations in consecutive-frame traffic scene maps. Moreover, the segmentation results of the greyscale input data sets are more stable and effective than using the RGB images for deep neural network feature extraction. The experimental results show that the proposed method takes advantage of the image features directly and achieves good real-time performance and high segmentation accuracy. 展开更多
关键词 Image Segmentation Local Region Condition random Field Model Deep Neural Network Consecutive Shooting Traffic Scene
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Influence of random shrinkage porosity on equivalent elastic modulus of casting: A statistical and numerical approach 被引量:1
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作者 Wei Liu Feng Li +1 位作者 Fuhua Yan Hui Wang 《China Foundry》 SCIE 2017年第2期108-120,共13页
Shrinkage porosity is a type of random distribution defects and exists in most large castings. Different from the periodic symmetry defects or certain distribution defects, shrinkage porosity presents a random "c... Shrinkage porosity is a type of random distribution defects and exists in most large castings. Different from the periodic symmetry defects or certain distribution defects, shrinkage porosity presents a random "cloud-like" configuration, which brings difficulties in quantifying the effective performance of defected casting. In this paper, the influences of random shrinkage porosity on the equivalent elastic modulus of QT400-18 casting were studied by a numerical statistics approach. An improved random algorithm was applied into the lattice model to simulate the "cloud-like" morphology of shrinkage porosity. Then, a large number of numerical samples containing random levels of shrinkage were generated by the proposed algorithm. The stress concentration factor and equivalent elastic modulus of these numerical samples were calculated. Based on a statistical approach, the effects of shrinkage porosity's distribution characteristics, such as area fraction, shape, and relative location on the casting's equivalent mechanical properties were discussed respectively. It is shown that the approach with randomly distributed defects has better predictive capabilities than traditional methods. The following conclusions can be drawn from the statistical simulations:(1) the effective modulus decreases remarkably if the shrinkage porosity percent is greater than 1.5%;(2) the average Stress Concentration Factor(SCF) produced by shrinkage porosity is about 2.0;(3) the defect's length across the loading direction plays a more important role in the effective modulus than the length along the loading direction;(4) the surface defect perpendicular to loading direction reduces the mean modulus about 1.5% more than a defect of other position. 展开更多
关键词 random lattice model equivalent elastic modulus shrinkage porosity defects casting
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A Comparative Study on Asperity Peak Modeling Methods
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作者 Wei Zhou Daiyan Zhao +1 位作者 Jinyuan Tang Jun Yi 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2021年第4期137-150,共14页
The peak identification scheme based method(three-point definition)and the spectral moments based method(spectral moment approach)are both widely used for asperity peak modeling in tribology.To discover the difference... The peak identification scheme based method(three-point definition)and the spectral moments based method(spectral moment approach)are both widely used for asperity peak modeling in tribology.To discover the differences between the two methods,a great number of rough surface profile samples with various statistical distributions are first randomly generated using FFT.Then the distribution parameters of asperity peaks are calculated for the generated samples with both methods.The obtained results are compared and verified by experiment.The variation rules of the differences between the two methods with statistical characteristics of rough surfaces are investigated.To explain for the discovered differences,the assumptions by spectral moment approach that the joint distribution of surface height,slope and curvature is normal and that the height distribution of asperities is Gaussian,are examined.The results show that it is unreasonable to assume a joint normal distribution without inspecting the correlation pattern of[z],[z′]and[z′′],and that the height distribution of asperities is not exactly Gaussian before correlation length of rough surface increases to a certain extent,20 for instance. 展开更多
关键词 Surface topography ROUGHNESS Surface analysis Asperity peak random process model
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Experimental study of population density using an optimized random forest model
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作者 LI Lingling LIU Jinsong +3 位作者 LI Zhi WEN Peizhang LI Yancheng LIU Yi 《Journal of Geographical Sciences》 SCIE CSCD 2024年第8期1636-1656,共21页
Random forest model is the mainstream research method used to accurately describe the distribution law and impact mechanism of regional population.We took Shijiazhuang as the research area,with comprehensive zoning ba... Random forest model is the mainstream research method used to accurately describe the distribution law and impact mechanism of regional population.We took Shijiazhuang as the research area,with comprehensive zoning based on endowments as the modeling unit,conducted stratified sampling on a hectare grid cell,and systematically carried out incremental selection experiments of population density impact factors,optimizing the population density random forest model throughout the process(zonal modeling,stratified sampling,factor selection,weighted output).The results are as follows:(1)Zonal modeling addresses the issue of confusion in population distribution laws caused by a single model.Sampling on a grid cell not only ensures the quality of training data by avoiding the modifiable areal unit problem(MAUP)but also attempts to mitigate the adverse effects of the ecological fallacy.Stratified sampling ensures the stability of population density label values(target variable)in the training sample.(2)Zonal selection experiments on population density impact factors help identify suitable combinations of factors,leading to a significant improvement in the goodness of fit(R^(2))of the zonal models.(3)Weighted combination output of the population density prediction dataset substantially enhances the model's robustness.(4)The population density dataset exhibits multi-scale superposition characteristics.On a large scale,the population density in plains is higher than that in mountainous areas,while on a small scale,urban areas have higher density compared to rural areas.The optimization scheme for the population density random forest model that we propose offers a unified technical framework for uncovering local population distribution law and the impact mechanisms. 展开更多
关键词 population density random forest model endowment zones stratified sampling factor selection weighted output
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Identification of Mixtures of Two Types of Body Fluids Using the Multiplex Methylation System and Random Forest Models
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作者 Han-xiao WANG Xiao-zhao LIU +3 位作者 Xi-miao HE Chao XIAO Dai-xin HUANG Shao-hua YI 《Current Medical Science》 SCIE CAS 2023年第5期908-918,共11页
Objective Body fluid mixtures are complex biological samples that frequently occur in crime scenes,and can provide important clues for criminal case analysis.DNA methylation assay has been applied in the identificatio... Objective Body fluid mixtures are complex biological samples that frequently occur in crime scenes,and can provide important clues for criminal case analysis.DNA methylation assay has been applied in the identification of human body fluids,and has exhibited excellent performance in predicting single-source body fluids.The present study aims to develop a methylation SNaPshot multiplex system for body fluid identification,and accurately predict the mixture samples.In addition,the value of DNA methylation in the prediction of body fluid mixtures was further explored.Methods In the present study,420 samples of body fluid mixtures and 250 samples of single body fluids were tested using an optimized multiplex methylation system.Each kind of body fluid sample presented the specific methylation profiles of the 10 markers.Results Significant differences in methylation levels were observed between the mixtures and single body fluids.For all kinds of mixtures,the Spearman’s correlation analysis revealed a significantly strong correlation between the methylation levels and component proportions(1:20,1:10,1:5,1:1,5:1,10:1 and 20:1).Two random forest classification models were trained for the prediction of mixture types and the prediction of the mixture proportion of 2 components,based on the methylation levels of 10 markers.For the mixture prediction,Model-1 presented outstanding prediction accuracy,which reached up to 99.3%in 427 training samples,and had a remarkable accuracy of 100%in 243 independent test samples.For the mixture proportion prediction,Model-2 demonstrated an excellent accuracy of 98.8%in 252 training samples,and 98.2%in 168 independent test samples.The total prediction accuracy reached 99.3%for body fluid mixtures and 98.6%for the mixture proportions.Conclusion These results indicate the excellent capability and powerful value of the multiplex methylation system in the identification of forensic body fluid mixtures. 展开更多
关键词 body fluid identification MIXTURE mixing ratio DNA methylation multiplex assay random forest model
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