[Objectives]To establish a multi-indicator quality control method for the retention of Longqing Capsule based on the principle of prescription of Chinese medicine.[Methods]High performance liquid chromatography(HPLC)w...[Objectives]To establish a multi-indicator quality control method for the retention of Longqing Capsule based on the principle of prescription of Chinese medicine.[Methods]High performance liquid chromatography(HPLC)with ShimNex CS C 18 as the column;column temperature:35℃;wavelength:270 nm;methanol-0.1%phosphoric acid solution as the mobile phase with gradient elution.[Results]The 12 components of the retention of Longqing Capsule showed good linearity within the investigated range(r≥0.9995),with the average spiked recoveries of 97.83%-100.52%and the RSD of 0.9%-2.1%.[Conclusions]The method is exclusive,sensitive,reproducible,simple and easy to use,and can provide a reference for the construction of the quality standard and control system of Longqing Capsule based on the theory of traditional Chinese medicine.展开更多
As a traditional herbal medicine,the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects.It is essential to develop an effective method for the determination ...As a traditional herbal medicine,the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects.It is essential to develop an effective method for the determination of the major alkaloids in U.rhynchophylla.In this research,a rapid quantitative analysis involving multi-components analysis by a single marker strategy coupled with core-shell column HPLC was adopted to analyse four alkaloids(corynoxeine,isocorynoxeine,isorhynchophylline,rhynchophylline)in U.rhynchophylla.Isorhynchophylline was selected as the internal reference substance,the content of which was determined by the traditional external standard method.Relative correction factors(RCF)between isorhynchophylline and the other three alkaloids were calculated respectively.The results showed that the QAMS method had good robustness under different HPLC instruments.Nineteen batches of U.rhynchophylla were tested.No significant difference was observed between the results by QAMS and EMS(Correlation coefficient>0.99,p>0.05).The QAMS method could be employed as a rapid,effective technique for the quality control of U.rhynchophylla.展开更多
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenolic acids,salvianolic acid B,tanshinol,lithospermic acid a...[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenolic acids,salvianolic acid B,tanshinol,lithospermic acid and rosmarinic acid in Radix Salviae Miltiorrhizae by HPLC and conduct methodological investigation.[Methods]Salvianolic acid B,with moderate retention time,good resolution,great response value and easy availability was selected as the internal standard,and the relative retention values and relative correction factors between tanshinol,rosmarinic acid,lithospermic acid and salvianolic acid B were established.The results of QAMS were compared with those of external standard method to verify the accuracy and applicability of QAMS.[Results]There were no significant differences between the calculated values of QAMS and the measured values of external standard method.The relative deviation was less than 3%,and the relative correction factors obtained from the experiment were credible.[Conclusions]QAMS can be used to determine the contents of phenolic acids in Radix Salviae Miltiorrhizae formula granules.展开更多
[Objectives] The research aimed to evaluate the quality of Zhenrongdan mixture by fingerprint combining QAMS. [Methods] The quality evaluation method was established and validated with echinacoside as internal referen...[Objectives] The research aimed to evaluate the quality of Zhenrongdan mixture by fingerprint combining QAMS. [Methods] The quality evaluation method was established and validated with echinacoside as internal reference to determine the contents of other components(ferulic acid, salvianolic acid B, and icariin) according to the relative correction factor. The accuracy and feasibility of QAMS were evaluated by comparison on the results between the measured value and calculation value by external standard method and QAMS. [Results] A common pattern of characteristic fingerprint of Zhenrongdan mixture by HPLC was established. Thirteen common peaks were identified, and they account for 91% of the total peak area, and four components were verified in five batches of Zhenrongdan mixture. Good similarities with correlation coefficients higher than 0.99 were found in the fingerprints. There was no significant difference between the quantitative results of the four ingredients in the five batches by external standard method and QAMS. [Conclusions] The method of fingerprint combined with QAMS could be used for the quality control of multiple components determination and fingerprint chromatography for Zhenrongdan mixture.展开更多
[Objectives]Chromatographic fingerprint analysis technology and quantitative analysis of multi-components by singlemarker(QAMS)were used to identify the authenticity and multi-component quantitative analysis ofStrepto...[Objectives]Chromatographic fingerprint analysis technology and quantitative analysis of multi-components by singlemarker(QAMS)were used to identify the authenticity and multi-component quantitative analysis ofStreptocaulon griffithiiHook,which provided experimental reference for the quality evaluation and control ofS.griffithiiHook.[Methods]Method was carried out on Agilent ZORBAX SB-C18(150 mm×4.6 mm,5μm)column with mobile phase composed of methanol-0.2%phosphoric acid solution at a flow rate of 1.0 mL/min in gradient elution mode.The column temperature was maintained at 30℃,the injection volume was 5μL,and the detection wavelengths were set at 230 nm.The HPLC fingerprint ofS.griffithiiHook was established byFingerprint Similarity Evaluation Software of Traditional Chinese Med-i cine(2012 edition),and the quality of 11 batches ofS.griffithiiHook extracts was analyzed.The content of chlorogenic acid,caffeic acid and4-methoxysalicylaldehyde inS.griffithiiHook was determined by QAMS.[Results]Thirteen common peaks were identified in the extract ofS.g riffithiiHook,and three components were identified as chlorogenic acid,caffeic acid and 4-methoxysalicylaldehyde,there was no significant difference between QAMS method and external standard method with chlorogenic acid as reference substance.[Conclusions]The established HPLC method is specific,accurate,stable and reproducible,and it can be used as an effective method for the quality control ofS.griffithii Hook.展开更多
Objective:To establish an high performance liquid chromatography-quantitative analysis of multi components by single marker(HPLC-QAMS)method for simultaneous determination of gastrodin,parishin E,parishin B,parishin,t...Objective:To establish an high performance liquid chromatography-quantitative analysis of multi components by single marker(HPLC-QAMS)method for simultaneous determination of gastrodin,parishin E,parishin B,parishin,tenuifolin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andαasarone in Dianxiankang Capsules.Methods:Waters Symmetry C_(18)column was used with acetonitrile-0.05%phosphoric acid solution as mobile phase for gradient elution.Multiwavelength switching detection.The contents of gastrodin,parishin E,parishin B,parishin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andα-asarone were calculated by relative correction factor.At the same time,the contents of 10 components in 12 batches of Dianxiankang Capsules were determined by external standard method(ESM).Results:An HPLC-QAMS method was established tenuifolin as the internal reference substance was established.The relative correction factors of gastrodin,parishin E,parishin B,parishin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andα-asarone were 0.8238,0.7239,1.0229,1.1881,0.7272,1.3108,0.9314,0.6549 and 1.0572,respectively.The relative correction factors had good repeatability and no significant difference with ESM(P>0.05).Conclusion:HPLC-QAMS can be used for simultaneous determination of multi-index components in Dianxiankang Capsules.展开更多
[Objectives]To regulate the quality of Wanjinxiang Shushuang Ointment through simultaneously quantifying menthol,camphor,1,8-cineole,linalool,borneol and caryophyllene oxide by QAMS(quantitative analysis of multi-comp...[Objectives]To regulate the quality of Wanjinxiang Shushuang Ointment through simultaneously quantifying menthol,camphor,1,8-cineole,linalool,borneol and caryophyllene oxide by QAMS(quantitative analysis of multi-components by single-marker).[Methods]The method was performed using an Agilent DB-WAX(30 m×0.32 mm,0.25μm)polyethylene glycol chromatographic column;with nitrogen employed as a carrier gas.The constant pressure was 4.73 psi;and the injection temperature was 240℃,with a shunt ratio:of 10:1;The hydrogen flame ion detector with a detector temperature of 240℃;the injection volume was 0.3μL.To verify the accuracy and applicability of QAMS,the results were compared with those obtained using the internal standard method(naphthalene).[Results]In Wanjinxiang Shushuang Ointment,menthol,camphor,1,8-cineole,linalool,borneol,caryophyllene oxide and naphthalene were well separated by the same chromatography with good linearity in their respective ranges(R≥0.9992).The average recoveries were 99.66%,101.03%,98.07%,98.24%,101.39%,and 103.39%with RSD s of 0.69%,1.52%,1.25%,1.94%,1.44%,and 2.74%,respectively.The QAMS is similar to the internal standard method.[Conclusions]This simple,accurate method with high precision,separation and reproducibility can serve as a reference for the quality control of Wanjinxiang Shushuang Ointment.展开更多
基金Supported by Provincial University Scientific Research Platform Team Project of Guizhou Provincial Department of Education(Qianjiaoji[2022]No.010).
文摘[Objectives]To establish a multi-indicator quality control method for the retention of Longqing Capsule based on the principle of prescription of Chinese medicine.[Methods]High performance liquid chromatography(HPLC)with ShimNex CS C 18 as the column;column temperature:35℃;wavelength:270 nm;methanol-0.1%phosphoric acid solution as the mobile phase with gradient elution.[Results]The 12 components of the retention of Longqing Capsule showed good linearity within the investigated range(r≥0.9995),with the average spiked recoveries of 97.83%-100.52%and the RSD of 0.9%-2.1%.[Conclusions]The method is exclusive,sensitive,reproducible,simple and easy to use,and can provide a reference for the construction of the quality standard and control system of Longqing Capsule based on the theory of traditional Chinese medicine.
文摘As a traditional herbal medicine,the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects.It is essential to develop an effective method for the determination of the major alkaloids in U.rhynchophylla.In this research,a rapid quantitative analysis involving multi-components analysis by a single marker strategy coupled with core-shell column HPLC was adopted to analyse four alkaloids(corynoxeine,isocorynoxeine,isorhynchophylline,rhynchophylline)in U.rhynchophylla.Isorhynchophylline was selected as the internal reference substance,the content of which was determined by the traditional external standard method.Relative correction factors(RCF)between isorhynchophylline and the other three alkaloids were calculated respectively.The results showed that the QAMS method had good robustness under different HPLC instruments.Nineteen batches of U.rhynchophylla were tested.No significant difference was observed between the results by QAMS and EMS(Correlation coefficient>0.99,p>0.05).The QAMS method could be employed as a rapid,effective technique for the quality control of U.rhynchophylla.
基金Supported by High-level Entrepreneurial and Innovative Talent(Team)Project 2015.
文摘[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenolic acids,salvianolic acid B,tanshinol,lithospermic acid and rosmarinic acid in Radix Salviae Miltiorrhizae by HPLC and conduct methodological investigation.[Methods]Salvianolic acid B,with moderate retention time,good resolution,great response value and easy availability was selected as the internal standard,and the relative retention values and relative correction factors between tanshinol,rosmarinic acid,lithospermic acid and salvianolic acid B were established.The results of QAMS were compared with those of external standard method to verify the accuracy and applicability of QAMS.[Results]There were no significant differences between the calculated values of QAMS and the measured values of external standard method.The relative deviation was less than 3%,and the relative correction factors obtained from the experiment were credible.[Conclusions]QAMS can be used to determine the contents of phenolic acids in Radix Salviae Miltiorrhizae formula granules.
文摘[Objectives] The research aimed to evaluate the quality of Zhenrongdan mixture by fingerprint combining QAMS. [Methods] The quality evaluation method was established and validated with echinacoside as internal reference to determine the contents of other components(ferulic acid, salvianolic acid B, and icariin) according to the relative correction factor. The accuracy and feasibility of QAMS were evaluated by comparison on the results between the measured value and calculation value by external standard method and QAMS. [Results] A common pattern of characteristic fingerprint of Zhenrongdan mixture by HPLC was established. Thirteen common peaks were identified, and they account for 91% of the total peak area, and four components were verified in five batches of Zhenrongdan mixture. Good similarities with correlation coefficients higher than 0.99 were found in the fingerprints. There was no significant difference between the quantitative results of the four ingredients in the five batches by external standard method and QAMS. [Conclusions] The method of fingerprint combined with QAMS could be used for the quality control of multiple components determination and fingerprint chromatography for Zhenrongdan mixture.
基金Supported by Scientific Research Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GZZC2019147)2020 Guangxi University Young and Middle-aged Teachers'Basic Scientific Research Ability Improvement Project(2020KY13034)+1 种基金the First Scientific Research Project of High-level Talents in the Affiliated Hospital of Youjiang Medical University for Nationalities in 2019(Y20196311)Scientific Research Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(20210241)。
文摘[Objectives]Chromatographic fingerprint analysis technology and quantitative analysis of multi-components by singlemarker(QAMS)were used to identify the authenticity and multi-component quantitative analysis ofStreptocaulon griffithiiHook,which provided experimental reference for the quality evaluation and control ofS.griffithiiHook.[Methods]Method was carried out on Agilent ZORBAX SB-C18(150 mm×4.6 mm,5μm)column with mobile phase composed of methanol-0.2%phosphoric acid solution at a flow rate of 1.0 mL/min in gradient elution mode.The column temperature was maintained at 30℃,the injection volume was 5μL,and the detection wavelengths were set at 230 nm.The HPLC fingerprint ofS.griffithiiHook was established byFingerprint Similarity Evaluation Software of Traditional Chinese Med-i cine(2012 edition),and the quality of 11 batches ofS.griffithiiHook extracts was analyzed.The content of chlorogenic acid,caffeic acid and4-methoxysalicylaldehyde inS.griffithiiHook was determined by QAMS.[Results]Thirteen common peaks were identified in the extract ofS.g riffithiiHook,and three components were identified as chlorogenic acid,caffeic acid and 4-methoxysalicylaldehyde,there was no significant difference between QAMS method and external standard method with chlorogenic acid as reference substance.[Conclusions]The established HPLC method is specific,accurate,stable and reproducible,and it can be used as an effective method for the quality control ofS.griffithii Hook.
基金National Traditional Chinese Medicine Technology Inheritance Talent Training Project(NO.T20194828003)。
文摘Objective:To establish an high performance liquid chromatography-quantitative analysis of multi components by single marker(HPLC-QAMS)method for simultaneous determination of gastrodin,parishin E,parishin B,parishin,tenuifolin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andαasarone in Dianxiankang Capsules.Methods:Waters Symmetry C_(18)column was used with acetonitrile-0.05%phosphoric acid solution as mobile phase for gradient elution.Multiwavelength switching detection.The contents of gastrodin,parishin E,parishin B,parishin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andα-asarone were calculated by relative correction factor.At the same time,the contents of 10 components in 12 batches of Dianxiankang Capsules were determined by external standard method(ESM).Results:An HPLC-QAMS method was established tenuifolin as the internal reference substance was established.The relative correction factors of gastrodin,parishin E,parishin B,parishin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andα-asarone were 0.8238,0.7239,1.0229,1.1881,0.7272,1.3108,0.9314,0.6549 and 1.0572,respectively.The relative correction factors had good repeatability and no significant difference with ESM(P>0.05).Conclusion:HPLC-QAMS can be used for simultaneous determination of multi-index components in Dianxiankang Capsules.
基金Supported by Projects of Guizhou Provincial Department of Education-Growth Program of Young Scientific and Technological Talents in Guizhou Ordinary Higher Education Institutions(QJHKY[2022]264)Rolling Support for Provincial University Research Platform Team Projects(QJJ[2022]010).
文摘[Objectives]To regulate the quality of Wanjinxiang Shushuang Ointment through simultaneously quantifying menthol,camphor,1,8-cineole,linalool,borneol and caryophyllene oxide by QAMS(quantitative analysis of multi-components by single-marker).[Methods]The method was performed using an Agilent DB-WAX(30 m×0.32 mm,0.25μm)polyethylene glycol chromatographic column;with nitrogen employed as a carrier gas.The constant pressure was 4.73 psi;and the injection temperature was 240℃,with a shunt ratio:of 10:1;The hydrogen flame ion detector with a detector temperature of 240℃;the injection volume was 0.3μL.To verify the accuracy and applicability of QAMS,the results were compared with those obtained using the internal standard method(naphthalene).[Results]In Wanjinxiang Shushuang Ointment,menthol,camphor,1,8-cineole,linalool,borneol,caryophyllene oxide and naphthalene were well separated by the same chromatography with good linearity in their respective ranges(R≥0.9992).The average recoveries were 99.66%,101.03%,98.07%,98.24%,101.39%,and 103.39%with RSD s of 0.69%,1.52%,1.25%,1.94%,1.44%,and 2.74%,respectively.The QAMS is similar to the internal standard method.[Conclusions]This simple,accurate method with high precision,separation and reproducibility can serve as a reference for the quality control of Wanjinxiang Shushuang Ointment.
