Two bacterial blight （BB） resistance genes, Xa21 and Xa4, from IRBB24 were introduced into hybrid rice restorer line Mianhui 725, which is highly susceptible to BB, by using hybridization and molecular marker-assist...Two bacterial blight （BB） resistance genes, Xa21 and Xa4, from IRBB24 were introduced into hybrid rice restorer line Mianhui 725, which is highly susceptible to BB, by using hybridization and molecular marker-assisted selection technology. Four homologous restorer lines were obtained through testing the R target genes with molecular markers and analyzing parental genetic background. Inoculation of the four lines and their hybrids with the specific strains of Xanthomonas oryzae pv. oryzae, P1, P6 and seven representative strains of Chinese pathotype, C Ⅰ -CⅦ, showed that all of the four lines and their hybrids were highly resistant and presented broad resistance-spectrum to BB. The hybrids of G46A / R207-2 displayed good agronomic characters and high yield potential, and R207-2 was named Shuhui 207.展开更多
Acoustic measurements or ultrasonic testing can be strongly affected by reflections or echoes from test tank walls. In order to create a non-reflecting environment equivalent to infinite medium, a pyramidal structured...Acoustic measurements or ultrasonic testing can be strongly affected by reflections or echoes from test tank walls. In order to create a non-reflecting environment equivalent to infinite medium, a pyramidal structured absorber (PSA) can be used to coat the walls of an ultrasonic tank. In this work, we model an array of tetragonal pyramid ultrasonic wave absorbers. This model is based on two coupled first-order equations describing the stress and particle velocity within an isotropic medium. For absorbing media, the Kelvin-Voigt model of viscoelasticity is used. The equations are discretized in 2D using an efficient time-stepping pseudo-spectral scheme that takes in consideration both, the acoustic properties and attenuation characteristics of the composite materials. We then built a 3D printed PSA using a Stratasys Objet500 Connex 3D printer, which allows to combine photopolymers in specific concentrations and microstructures. We designed PSA covering the frequency ranges from 0.5 MHz to 5 MHz and from 1 MHz to 10 MHz, with double homogeneous layer: a core of rubber material with a skin of a variety of elastomers by combining rigid and flexible materials. Each single pyramid contains two major parts: the ground of the pyramid (9.4 mm base × 4.7 mm height, for 0.5 MHz and 4.7 mm base × 2.35 mm height, for 1 MHz) and the body of the pyramid (23.5 mm height, for 0.5 MHZ and 11.75 mm height, for 1 MHz). The measured echo-reduction was greater than 35 dB at the covering frequency range and the transmission loss was estimated by 20 dB. Echoes increase rapidly for frequencies below the minimum frequency of the covering range. The modeling and 3D printing of PSA with different sizes, in a wide range of frequencies, is a cost-effective custom solution for a wide range of applications including for example, radiation force balances, hydrophone mounts and medical ultrasound equipment.展开更多
Genetic improvement is one of the most effective strategies to prevent rice from blast and bacterial blight （BB） diseases, the two most prevalent diseases jeopardizing rice production. Rice hybrids with dural resist...Genetic improvement is one of the most effective strategies to prevent rice from blast and bacterial blight （BB） diseases, the two most prevalent diseases jeopardizing rice production. Rice hybrids with dural resistance to blast and BB are needed for sustainable production of food. An incomplete diallele design resulted in 25 crosses between five blast and five BB resistant germplasm accessions. Only one pair of parents, DH146 ×TM487, showed polymorphism for all the markers to identify one blast resistance gene Pi25 and three BB resistance genes, Xa21, xa13 and xa5, thus it was used in the marker-assisted selection （MAS）. F2 individuals of DH146× TM487 were genotyped using flanking markers of RM3330 and sequence tagged site （STS） marker SA7 for Pi25. The resistant F2 plants with Pi25 were used for pyramiding BB resistance genes Xa21, xa13 and xa5 identified by the markers pTA248, RM264 and RM153, respectively in subsequent generations. Finally, after selection for agronomic traits and restoration ability among 12 pyramided lines, we acquired an elite restorer line, R8012 including all four target genes （Pi25＋Xa21＋xa13＋xa5）. Hybrid Zhong 9NR8012 derived from the selected line showed stronger resistance to blast and BB, and higher grain yield than the commercial checks uniformally in experimental plots, 2007 state-wide yield trial and 2008 nation-wide yield trial. This study provides a paradigmatic example to show that MAS is a practically feasible tool in effectively pyramiding multiple resistance genes. The resultant restoring line and its hybrid would play an important role in securing rice production in China.展开更多
文摘Two bacterial blight （BB） resistance genes, Xa21 and Xa4, from IRBB24 were introduced into hybrid rice restorer line Mianhui 725, which is highly susceptible to BB, by using hybridization and molecular marker-assisted selection technology. Four homologous restorer lines were obtained through testing the R target genes with molecular markers and analyzing parental genetic background. Inoculation of the four lines and their hybrids with the specific strains of Xanthomonas oryzae pv. oryzae, P1, P6 and seven representative strains of Chinese pathotype, C Ⅰ -CⅦ, showed that all of the four lines and their hybrids were highly resistant and presented broad resistance-spectrum to BB. The hybrids of G46A / R207-2 displayed good agronomic characters and high yield potential, and R207-2 was named Shuhui 207.
文摘Acoustic measurements or ultrasonic testing can be strongly affected by reflections or echoes from test tank walls. In order to create a non-reflecting environment equivalent to infinite medium, a pyramidal structured absorber (PSA) can be used to coat the walls of an ultrasonic tank. In this work, we model an array of tetragonal pyramid ultrasonic wave absorbers. This model is based on two coupled first-order equations describing the stress and particle velocity within an isotropic medium. For absorbing media, the Kelvin-Voigt model of viscoelasticity is used. The equations are discretized in 2D using an efficient time-stepping pseudo-spectral scheme that takes in consideration both, the acoustic properties and attenuation characteristics of the composite materials. We then built a 3D printed PSA using a Stratasys Objet500 Connex 3D printer, which allows to combine photopolymers in specific concentrations and microstructures. We designed PSA covering the frequency ranges from 0.5 MHz to 5 MHz and from 1 MHz to 10 MHz, with double homogeneous layer: a core of rubber material with a skin of a variety of elastomers by combining rigid and flexible materials. Each single pyramid contains two major parts: the ground of the pyramid (9.4 mm base × 4.7 mm height, for 0.5 MHz and 4.7 mm base × 2.35 mm height, for 1 MHz) and the body of the pyramid (23.5 mm height, for 0.5 MHZ and 11.75 mm height, for 1 MHz). The measured echo-reduction was greater than 35 dB at the covering frequency range and the transmission loss was estimated by 20 dB. Echoes increase rapidly for frequencies below the minimum frequency of the covering range. The modeling and 3D printing of PSA with different sizes, in a wide range of frequencies, is a cost-effective custom solution for a wide range of applications including for example, radiation force balances, hydrophone mounts and medical ultrasound equipment.
基金supported in part by the National Natural Science Foundation of China (Grant No. 30623006,No. 31101209)the National High Technology Research and Development Program of China (Grant No.2006AA10Z1E8)the Program of Introducing International Advanced Agricultural Science and Technologies (948 Program) of Ministry of Agricultureof China (Grant No. 2006-G51)
文摘Genetic improvement is one of the most effective strategies to prevent rice from blast and bacterial blight （BB） diseases, the two most prevalent diseases jeopardizing rice production. Rice hybrids with dural resistance to blast and BB are needed for sustainable production of food. An incomplete diallele design resulted in 25 crosses between five blast and five BB resistant germplasm accessions. Only one pair of parents, DH146 ×TM487, showed polymorphism for all the markers to identify one blast resistance gene Pi25 and three BB resistance genes, Xa21, xa13 and xa5, thus it was used in the marker-assisted selection （MAS）. F2 individuals of DH146× TM487 were genotyped using flanking markers of RM3330 and sequence tagged site （STS） marker SA7 for Pi25. The resistant F2 plants with Pi25 were used for pyramiding BB resistance genes Xa21, xa13 and xa5 identified by the markers pTA248, RM264 and RM153, respectively in subsequent generations. Finally, after selection for agronomic traits and restoration ability among 12 pyramided lines, we acquired an elite restorer line, R8012 including all four target genes （Pi25＋Xa21＋xa13＋xa5）. Hybrid Zhong 9NR8012 derived from the selected line showed stronger resistance to blast and BB, and higher grain yield than the commercial checks uniformally in experimental plots, 2007 state-wide yield trial and 2008 nation-wide yield trial. This study provides a paradigmatic example to show that MAS is a practically feasible tool in effectively pyramiding multiple resistance genes. The resultant restoring line and its hybrid would play an important role in securing rice production in China.
