The reproduction of grapevine genotypes,one of the most important species in the world,while preserving their genetic characteristics,is practically done by rooting cuttings.Adventive rooting of cutting studies for se...The reproduction of grapevine genotypes,one of the most important species in the world,while preserving their genetic characteristics,is practically done by rooting cuttings.Adventive rooting of cutting studies for seedling production in nursery conditions often remain below the expected productivity level due to biotic and abiotic stress-related reasons.Studies to increase nursery yields are still on the agenda of grapevine researchers.In this study,the effects of silver nanoparticles(AgNPs)produced by the green synthesis method using grape seed extract and AgNO3 on rooting and vegetative growth of the standard(TS 4027)cuttings taken during the dormancy period of Vitis vinifera L.cvs Ekşi Kara and GökÜzüm were investigated under greenhouse conditions.Cuttings treated by keeping in 0.1,0.2 g·L^(−1)AgNPs,0.1,0.2 g·L^(−1)IBA aqueous solutions for 24 h were planted in black,1 L volume seedling bags filled with 1:1 peat:perlite in the greenhouse,while the control was kept in pure water for 24 h and planted.Changes in sprouting rate,plant transformation rate,shoot length,shoot diameter,number of nodes,stomatal conductance,leaf temperature,photosynthetic efficiency,leaf fresh and dry weight,SPAD,root number,root length,root fresh and root weight were examined in developing seedlings.In evaluating the effects of AgNPs and Indole-3-butyric acid(IBA)treatments on cutting rooting and vegetative development,ANOVA,post hoc analysis with the Tukey test,and Principal Component Analyses(PCAs)were used to better understand and depict the correlations between the examined variables.This analysis method was performed using ggplot2 in the R Studio program.The heatmap generated by the pheatmap package was used to visualize the correlation and variation.As a result of this study,AgNPs applications were found to be more effective than IBA treatments in the rooting of grapevine cuttings and the vegetative development of young plants.In conclusion,0.1 g·L^(−1)AgNPs can be tested as a support and/or economical alternative to IBA for the promotion of rooting of cuttings and vegetative development of young plants for subsequent clonal propagation.展开更多
In order to reduce the non-point source pollution caused by the large or excessive application of chemical fertilizers and pesticides,this study was aimed to develop new alternative functional substances.The effects o...In order to reduce the non-point source pollution caused by the large or excessive application of chemical fertilizers and pesticides,this study was aimed to develop new alternative functional substances.The effects of different concentrations of indolebutyric acid-lanthanum chelate on seed germination and tomato seedling growth were studied by germination test of cucumber and tomato seeds and pot experiment of tomato seedlings.The results showed that the optimum concentration of indolebutyric acid-lanthanum chelate could promote the germination and growth of tomato and cucumber seeds.Among them,0.02 mg/L of indolebutyric acid-lanthanum chelate is the optimum concentration for tomato and cucumber seed germination.When the concentration is 0.1 mg/L,it is the optimum concentration for radicle and hypocotyl growth.In the pot experiment,when the optimum concentration of indolebutyric acid-lanthanum chelate on tomato seedlings was 0.5 mg/L,the plant height,stem diameter,aboveground biomass,underground biomass and leaf area of tomato seedlings could be significantly promoted.At the same time,the absorption of the main elements P,K,Mg and trace elements Fe,Mn,Cu,Zn and Na and their transfer from the underground part to the aboveground part of the seedlings were enhanced.The relative expression of the tomato expansion protein genes leEXP2,leEXP18 and leEXP5 was also significantly increased.Therefore,the suitable concentration of indolebutyric acid-lanthanum chelate has the effect of promoting root growth and promoting the quality of tomato seedlings.展开更多
文摘The reproduction of grapevine genotypes,one of the most important species in the world,while preserving their genetic characteristics,is practically done by rooting cuttings.Adventive rooting of cutting studies for seedling production in nursery conditions often remain below the expected productivity level due to biotic and abiotic stress-related reasons.Studies to increase nursery yields are still on the agenda of grapevine researchers.In this study,the effects of silver nanoparticles(AgNPs)produced by the green synthesis method using grape seed extract and AgNO3 on rooting and vegetative growth of the standard(TS 4027)cuttings taken during the dormancy period of Vitis vinifera L.cvs Ekşi Kara and GökÜzüm were investigated under greenhouse conditions.Cuttings treated by keeping in 0.1,0.2 g·L^(−1)AgNPs,0.1,0.2 g·L^(−1)IBA aqueous solutions for 24 h were planted in black,1 L volume seedling bags filled with 1:1 peat:perlite in the greenhouse,while the control was kept in pure water for 24 h and planted.Changes in sprouting rate,plant transformation rate,shoot length,shoot diameter,number of nodes,stomatal conductance,leaf temperature,photosynthetic efficiency,leaf fresh and dry weight,SPAD,root number,root length,root fresh and root weight were examined in developing seedlings.In evaluating the effects of AgNPs and Indole-3-butyric acid(IBA)treatments on cutting rooting and vegetative development,ANOVA,post hoc analysis with the Tukey test,and Principal Component Analyses(PCAs)were used to better understand and depict the correlations between the examined variables.This analysis method was performed using ggplot2 in the R Studio program.The heatmap generated by the pheatmap package was used to visualize the correlation and variation.As a result of this study,AgNPs applications were found to be more effective than IBA treatments in the rooting of grapevine cuttings and the vegetative development of young plants.In conclusion,0.1 g·L^(−1)AgNPs can be tested as a support and/or economical alternative to IBA for the promotion of rooting of cuttings and vegetative development of young plants for subsequent clonal propagation.
基金This work was supported by the“13th Five-Year Plan”National Key Research and Development Project.The authors wish to thank Chen Qin,Hu Lin and Cui Jianyu for helpful discussion and advice on the manuscript.
文摘In order to reduce the non-point source pollution caused by the large or excessive application of chemical fertilizers and pesticides,this study was aimed to develop new alternative functional substances.The effects of different concentrations of indolebutyric acid-lanthanum chelate on seed germination and tomato seedling growth were studied by germination test of cucumber and tomato seeds and pot experiment of tomato seedlings.The results showed that the optimum concentration of indolebutyric acid-lanthanum chelate could promote the germination and growth of tomato and cucumber seeds.Among them,0.02 mg/L of indolebutyric acid-lanthanum chelate is the optimum concentration for tomato and cucumber seed germination.When the concentration is 0.1 mg/L,it is the optimum concentration for radicle and hypocotyl growth.In the pot experiment,when the optimum concentration of indolebutyric acid-lanthanum chelate on tomato seedlings was 0.5 mg/L,the plant height,stem diameter,aboveground biomass,underground biomass and leaf area of tomato seedlings could be significantly promoted.At the same time,the absorption of the main elements P,K,Mg and trace elements Fe,Mn,Cu,Zn and Na and their transfer from the underground part to the aboveground part of the seedlings were enhanced.The relative expression of the tomato expansion protein genes leEXP2,leEXP18 and leEXP5 was also significantly increased.Therefore,the suitable concentration of indolebutyric acid-lanthanum chelate has the effect of promoting root growth and promoting the quality of tomato seedlings.
