BACKGROUND Recently,the identification of cell apoptosis induced by natural products has become research hotspot and frontier in the biopharmaceutical and food industries under the umbrella of global green development...BACKGROUND Recently,the identification of cell apoptosis induced by natural products has become research hotspot and frontier in the biopharmaceutical and food industries under the umbrella of global green development worldwide.Traditionally,cell apoptosis is identified using morphological,biochemical,and cell cycle experiments,which is time consuming,and experimental materials are not from the same group,and it is very hard to ensure the identity and veracity of results of former and latter experiments.AIM To establish a selective,instant,and practical protocol to identify cell apoptosis induced by natural products.METHODS A one transient cell processing procedure(OTCPP)was used to detect human colorectal cancer LoVo cell apoptosis after treatment with Pinus massoniana bark extract(PMBE)at the morphological,biochemical,and cell cycle levels.The methods used included treatment with DNA gel electrophoresis,fluorescence microscopy,and flow cytometry.RESULTS In PMBE-treated LoVo cells,we observed a DNA ladder on gel electrophoresis and fluorescence microscopy revealed"nuclear shrinkage,chromatin condensation or fragmentation".In addition,flow cytometry showed an"obvious apoptosis curve".Thus OTCPP achieved synchronous detection of the morphology,biochemistry,cell cycle,and the DNA content of the cells.CONCLUSION OTCPP can quickly identify apoptosis and measure the apoptosis rate,thereby unifying qualitative and quantitative analysis.展开更多
A new algorithm is proposed to determine the actual length and the number of the protruding fibres of yarn based on a combination of image acquisition technology. First, a yarn hairiness image is obtained by the serie...A new algorithm is proposed to determine the actual length and the number of the protruding fibres of yarn based on a combination of image acquisition technology. First, a yarn hairiness image is obtained by the series of image processing procedures that include grayscale transformation,skew correction,yarn binary image acquisition and yarn core binary image obtaining. Then,the hairiness is realized in single pixel width by the usage of thinning algorithm. Finally, a baseline of yarn core margin is obtained,and pixels that match 8-neighbor template correctly are found by row scanning in a certain area. From this,these pixels are judged and the real crossover points of yarn core margin and hairiness,i. e.,the starting points of hairiness,are gained. The real length of the protruding fibres is calculated by tracking hairiness from the starting point constantly.展开更多
Yeast-driven alcoholic fermentation is widely applied to process fruits into value-added beverages.Fruit geno-types,yeast strains,and beverage production methods significantly influence the diversity and quality of al...Yeast-driven alcoholic fermentation is widely applied to process fruits into value-added beverages.Fruit geno-types,yeast strains,and beverage production methods significantly influence the diversity and quality of alco-holic beverages.Traditionally,Saccharomyces cerevisiae is regarded as the most commercially important yeast in the alcoholic beverage production markets,whereas other Saccharomyces and non-Saccharomyces is considered to be either harmful or useless.However,the interest of applying these yeasts in numerous innovative yeast fer-mented alcoholic fruit-based beverages is growing due to their advantageous flavor attributions.In this review,the strategies for enhancing volatile properties and aroma and flavor complexity of alcoholic beverage fermentation were critically examined with a special emphasis on fruit types and yeast strains,as well as their impacts on the quality of alcoholic beverages.The current challenges and future prospects were also discussed on the development of desirable alcoholic beverage production.展开更多
基金Supported by 2023 Key R&D Projects of Tongji University,No.150029607160-24323Industrial Key Program Foundation of Guangdong Province:R&D of Natural Novel Anticancer Drugs,No.2004B10401033。
文摘BACKGROUND Recently,the identification of cell apoptosis induced by natural products has become research hotspot and frontier in the biopharmaceutical and food industries under the umbrella of global green development worldwide.Traditionally,cell apoptosis is identified using morphological,biochemical,and cell cycle experiments,which is time consuming,and experimental materials are not from the same group,and it is very hard to ensure the identity and veracity of results of former and latter experiments.AIM To establish a selective,instant,and practical protocol to identify cell apoptosis induced by natural products.METHODS A one transient cell processing procedure(OTCPP)was used to detect human colorectal cancer LoVo cell apoptosis after treatment with Pinus massoniana bark extract(PMBE)at the morphological,biochemical,and cell cycle levels.The methods used included treatment with DNA gel electrophoresis,fluorescence microscopy,and flow cytometry.RESULTS In PMBE-treated LoVo cells,we observed a DNA ladder on gel electrophoresis and fluorescence microscopy revealed"nuclear shrinkage,chromatin condensation or fragmentation".In addition,flow cytometry showed an"obvious apoptosis curve".Thus OTCPP achieved synchronous detection of the morphology,biochemistry,cell cycle,and the DNA content of the cells.CONCLUSION OTCPP can quickly identify apoptosis and measure the apoptosis rate,thereby unifying qualitative and quantitative analysis.
基金National Natural Science Foundation of China(No.61301276)Xi’an Polytechnic University Young Scholar Backbone Supporting Plan,ChinaDiscipline Construction Funds of Xi’an Polytechnic University,China(No.107090811)
文摘A new algorithm is proposed to determine the actual length and the number of the protruding fibres of yarn based on a combination of image acquisition technology. First, a yarn hairiness image is obtained by the series of image processing procedures that include grayscale transformation,skew correction,yarn binary image acquisition and yarn core binary image obtaining. Then,the hairiness is realized in single pixel width by the usage of thinning algorithm. Finally, a baseline of yarn core margin is obtained,and pixels that match 8-neighbor template correctly are found by row scanning in a certain area. From this,these pixels are judged and the real crossover points of yarn core margin and hairiness,i. e.,the starting points of hairiness,are gained. The real length of the protruding fibres is calculated by tracking hairiness from the starting point constantly.
基金the financial supports from First Division Alar City Financial Science and Technology Plan Project(No.2024SP01)National Natural Science Foundation of China(No.32402151).
文摘Yeast-driven alcoholic fermentation is widely applied to process fruits into value-added beverages.Fruit geno-types,yeast strains,and beverage production methods significantly influence the diversity and quality of alco-holic beverages.Traditionally,Saccharomyces cerevisiae is regarded as the most commercially important yeast in the alcoholic beverage production markets,whereas other Saccharomyces and non-Saccharomyces is considered to be either harmful or useless.However,the interest of applying these yeasts in numerous innovative yeast fer-mented alcoholic fruit-based beverages is growing due to their advantageous flavor attributions.In this review,the strategies for enhancing volatile properties and aroma and flavor complexity of alcoholic beverage fermentation were critically examined with a special emphasis on fruit types and yeast strains,as well as their impacts on the quality of alcoholic beverages.The current challenges and future prospects were also discussed on the development of desirable alcoholic beverage production.
