Objective To study the effect of Chinese herbs preparation Bu-shen on pituitary prolactin-releasing peptide receptor (PrRP-R) and prolactin receptor (PRL-R) expression in the bromocriptine-induced rat abortion model M...Objective To study the effect of Chinese herbs preparation Bu-shen on pituitary prolactin-releasing peptide receptor (PrRP-R) and prolactin receptor (PRL-R) expression in the bromocriptine-induced rat abortion model Materials & Methods Female SD rats were divided into five groups randomly. Group A,B,C and D were injected with bromocriptine [0. 3 mg/(kg · d)] during the pregnant day 6～ 8, respectively. Group B was given powder of Bu-shen herbs [3 g/(kg · d)]. Group C was injected with prolactin (8 IU) twice a day and Group D with progesterone [(8 mg/kg · d) in the pregnant day 1～ 11. The rats in Group E were normal pregnant rats. All these rats were killed at the pregnant day 12 to observe the expression of PrRP-R, PRL-R from the pituitary using RT-PCR. Results The pregnant rate and total number of litters in Group A were significantly lower than those in Group B, C, D and E (P＜0. 01) and the expression of PrRP-R and PRL-R in the pituitary of Group A was higher than that of any other group. Conclusion Preparation Bu-shen plays a regulatory role in the secretion of prolactin possibly via modulating rats' hypothalamus and pituitary.展开更多
文摘Objective To study the effect of Chinese herbs preparation Bu-shen on pituitary prolactin-releasing peptide receptor (PrRP-R) and prolactin receptor (PRL-R) expression in the bromocriptine-induced rat abortion model Materials & Methods Female SD rats were divided into five groups randomly. Group A,B,C and D were injected with bromocriptine [0. 3 mg/(kg · d)] during the pregnant day 6～ 8, respectively. Group B was given powder of Bu-shen herbs [3 g/(kg · d)]. Group C was injected with prolactin (8 IU) twice a day and Group D with progesterone [(8 mg/kg · d) in the pregnant day 1～ 11. The rats in Group E were normal pregnant rats. All these rats were killed at the pregnant day 12 to observe the expression of PrRP-R, PRL-R from the pituitary using RT-PCR. Results The pregnant rate and total number of litters in Group A were significantly lower than those in Group B, C, D and E (P＜0. 01) and the expression of PrRP-R and PRL-R in the pituitary of Group A was higher than that of any other group. Conclusion Preparation Bu-shen plays a regulatory role in the secretion of prolactin possibly via modulating rats' hypothalamus and pituitary.
