近年来全球极端低温天气频发,严重影响了茶树的产量和品质。ICE(Inducer of CBF expression)基因家族主要参与植物的低温胁迫响应,但在茶树领域中的相关研究还不够全面。本研究从茶树基因组中鉴定出51个茶树CsICEs基因,对其理化性质、...近年来全球极端低温天气频发,严重影响了茶树的产量和品质。ICE(Inducer of CBF expression)基因家族主要参与植物的低温胁迫响应,但在茶树领域中的相关研究还不够全面。本研究从茶树基因组中鉴定出51个茶树CsICEs基因,对其理化性质、基因结构和启动子顺式作用元件展开生物信息学分析。茶树CsICEs基因的启动子区域富含光响应、植物激素、生长发育及非生物胁迫相关顺式作用元件,其可能参与多种逆境胁迫响应。转录组分析和RT-qPCR验证结果发现,低温下CsICE43基因的表达量上升了4.24倍,其可能与茶树低温响应相关。以茶树品种‘保靖黄金茶1号’的cDNA为模板,克隆获得了CsICE43基因,其在不同组织中的表达模式存在差异,在顶芽和嫩叶中特异性高表达。蛋白氨基酸序列和系统进化树分析表明,CsICE43基因包含与ICE家族其他成员一致的S-rich、bHLH、ACT等保守结构域,且与毛花猕猴桃(Actinidiaeriantha)的亲缘关系较近。在STRING在线网站中以拟南芥AtICEs为模型,推测茶树CsICE43蛋白与HOS1、MYB15、DREB1/2存在潜在的互作关系。亚细胞定位试验表明CsICE43定位于细胞核,与跨膜结构分析结果一致。综上所述,本研究发现CsICE43基因可能与茶树低温响应关联,为深入挖掘其基因功能与抗寒分子机理提供了一定的理论基础。展开更多
Although the phase-shift seismic processing method has characteristics of high accuracy, good stability, high efficiency, and high-dip imaging, it is not able to adapt to strong lateral velocity variation. To overcome...Although the phase-shift seismic processing method has characteristics of high accuracy, good stability, high efficiency, and high-dip imaging, it is not able to adapt to strong lateral velocity variation. To overcome this defect, a finite-difference method in the frequency-space domain is introduced in the migration process, because it can adapt to strong lateral velocity variation and the coefficient is optimized by a hybrid genetic and simulated annealing algorithm. The two measures improve the precision of the approximation dispersion equation. Thus, the imaging effect is improved for areas of high-dip structure and strong lateral velocity variation. The migration imaging of a 2-D SEG/EAGE salt dome model proves that a better imaging effect in these areas is achieved by optimized phase-shift migration operator plus a finite-difference method based on a hybrid genetic and simulated annealing algorithm. The method proposed in this paper is better than conventional methods in imaging of areas of high-dip angle and strong lateral velocity variation.展开更多
A tunable frequency-multiplying optoelectronic oscillator(OEO) based on a dual-parallel Mach-Zehnder modulator(DPMZM) is proposed and experimentally demonstrated. In the proposed system, the tunable fundamental microw...A tunable frequency-multiplying optoelectronic oscillator(OEO) based on a dual-parallel Mach-Zehnder modulator(DPMZM) is proposed and experimentally demonstrated. In the proposed system, the tunable fundamental microware signal is generated by a tunable optoelectronic oscillator incorporating a phase-shifted fiber Bragg grating(PS-FBG). By adjusting the DC bias of the DPMZM, the frequency-doubled microwave signal with a tunable frequency range from 11 GHz to 20 GHz and the frequency-quadrupled microwave signal with a tunable frequency range from 22.5 GHz to 26 GHz are generated. The phase noises of the fundamental, frequency-doubled and frequency-quadrupled signals at 10 k Hz offset frequency are-105.9 d Bc/Hz,-103.3 d Bc/Hz and-86.2 d Bc/Hz, respectively.展开更多
为探究ICE(inducer of CBF expression)转录因子在高丛蓝莓花果发育进程中可能的调控机制,通过对VcICE基因家族进行全基因组鉴定及生物信息学分析,采用实时荧光定量PCR(qRT-PCR)技术分析VcICE基因亚家族成员在蓝莓花芽膨大和果实发育进...为探究ICE(inducer of CBF expression)转录因子在高丛蓝莓花果发育进程中可能的调控机制,通过对VcICE基因家族进行全基因组鉴定及生物信息学分析,采用实时荧光定量PCR(qRT-PCR)技术分析VcICE基因亚家族成员在蓝莓花芽膨大和果实发育进程中的相对表达水平.结果表明:在高丛蓝莓‘Draper’基因组中鉴定出8个VcICE成员,系统进化分析将其划分为2个亚家族,各亚家族成员均包含S-rich,bHLH和ACT等保守结构域;VcICE基因启动子区域富含光响应、植物激素、生长发育及非生物胁迫相关顺式作用元件;VcICE亚家族基因在高丛蓝莓‘O’Neal’和‘Bluerain’花芽膨大与果实发育进程中的相对表达差异显著.研究结果以期为深入了解VcICE转录因子在蓝莓花果发育进程中的功能提供理论参考.展开更多
Phase and amplitude regeneration are necessary for degraded differential phase-shift keying communication sys- tems. This paper proposes a regenerator based on semiconductor optical amplifier for differential phase-sh...Phase and amplitude regeneration are necessary for degraded differential phase-shift keying communication sys- tems. This paper proposes a regenerator based on semiconductor optical amplifier for differential phase-shift keying signals. The key regeneration mechanism is theoretically analysed. The effectiveness of semiconductor optical amplifier based regenerator is demonstrated by comparing the bit error rate and eye diagrams before and after regeneration for 40-Cbit/s differential phase-shift keying 1080-km transmission systems. The results show that regeneration effects are very well. Bit error rate is tess than 10-12 with the regenerator.展开更多
We present a scheme to realize geometric phase-shift gate for two superconducting quantum interference device （SQUID） qubits coupled to a single-mode microwave field. The geometric phase-shift gate operation is perf...We present a scheme to realize geometric phase-shift gate for two superconducting quantum interference device （SQUID） qubits coupled to a single-mode microwave field. The geometric phase-shift gate operation is performed in two lower flux states, and the excited state [2〉 would not participate in the procedure. The SQUIDs undergo no transitions during the gate operation. Thus, the docoherence due to energy spontaneous emission based on the levels of SQUIDs are suppressed. The gate is insensitive to the cavity decay throughout the operation since the cavity mode is displaced along a circle in the phase space, acquiring a phase conditional upon the two lower flux states of the SQUID qubits, and the cavity mode is still in the original vacuum state. Based on the SQUID qubits interacting with the cavity mode, our proposed approach may open promising prospects for quantum iogic in SQUID-system.展开更多
Broad-band all-optical wavelength conversion of differential phase-shift keyed （DPSK） signal is experimentally demonstrated. This scheme is composed of a one-bit delay interferometer demodulation stage followed by a...Broad-band all-optical wavelength conversion of differential phase-shift keyed （DPSK） signal is experimentally demonstrated. This scheme is composed of a one-bit delay interferometer demodulation stage followed by a semiconductor optical amplifier （SOA） based nonlinear polarization switch. A wavelength converter for the 10 G b/s DPSK signal is presented, which has a wide wavelength range of more than 30 nm. The converted signals experience small power penalties less than 1.4 dB compared with the original signal, at a bit error rate of 10-9. Additionally, the optical spectra, the measured waveforms and the open eye diagrams of the converted signals show a high quality wavelength conversion performance.展开更多
focus of all countries.As an effective new energy,the fuel cell has attracted the attention of scholars.However,due to the particularity of proton exchange membrane fuel cell(PEMFC),the performance of traditional PI c...focus of all countries.As an effective new energy,the fuel cell has attracted the attention of scholars.However,due to the particularity of proton exchange membrane fuel cell(PEMFC),the performance of traditional PI controlled phase-shifted full-bridge power electronics DC-DC converter cannot meet the needs of practical application.In order to further improve the dynamic performance of the converter,this paper first introduces several main topologies of the current mainstream front-end DC-DC converter,and analyzes their performance in the fuel cell system.Then,the operation process of the phase-shifted fullbridge power electronics DC-DC converter is introduced,and the shortcomings of the traditional PI control are analyzed.Finally,a double closed-loop adaptive fuzzy PI controller is proposed,which is characterized by dynamically adjusting PI parameters according to different working states to complete the intelligent control of phase-shifted full-bridge DC-DC converter.The simulation results in MATLAB/Simulink show that the proposed algorithm has good a control effect.Compared with the traditional algorithm,the overshoot and stabilization time of the system are shorter.The algorithm can effectively suppress the fluctuation of the output current of the fuel cell converter,and is a very practical control method.展开更多
Recently, a novel kind of quantum key distribution called the round-robin differential phase-shift （RRDPS） protocol was proposed, which bounds the amount of leakage without monitoring signal disturbance. The protoco...Recently, a novel kind of quantum key distribution called the round-robin differential phase-shift （RRDPS） protocol was proposed, which bounds the amount of leakage without monitoring signal disturbance. The protocol can be implemented by a weak coherent source. The security of this protocol with a simply characterized source has been proved. The application of a common phase shift can improve the secret key rate of the protocol. In practice, the randomized phase is discrete and the secret key rate is deviated from the continuous case. In this study, we analyze security of the RRDPS protocol with discrete-phase-randomized coherent state source and bound the secret key rate. We fix the length of each packet at 32 and 64, then simulate the secret key rates of the RRDPS protocol with discrete-phase randomization and continuous-phase randomization. Our simulation results show that the performance of the discrete-phase randomization case is close to the continuous counterpart with only a small number of discrete phases. The research is practically valuable for experimental implementation.展开更多
文摘近年来全球极端低温天气频发,严重影响了茶树的产量和品质。ICE(Inducer of CBF expression)基因家族主要参与植物的低温胁迫响应,但在茶树领域中的相关研究还不够全面。本研究从茶树基因组中鉴定出51个茶树CsICEs基因,对其理化性质、基因结构和启动子顺式作用元件展开生物信息学分析。茶树CsICEs基因的启动子区域富含光响应、植物激素、生长发育及非生物胁迫相关顺式作用元件,其可能参与多种逆境胁迫响应。转录组分析和RT-qPCR验证结果发现,低温下CsICE43基因的表达量上升了4.24倍,其可能与茶树低温响应相关。以茶树品种‘保靖黄金茶1号’的cDNA为模板,克隆获得了CsICE43基因,其在不同组织中的表达模式存在差异,在顶芽和嫩叶中特异性高表达。蛋白氨基酸序列和系统进化树分析表明,CsICE43基因包含与ICE家族其他成员一致的S-rich、bHLH、ACT等保守结构域,且与毛花猕猴桃(Actinidiaeriantha)的亲缘关系较近。在STRING在线网站中以拟南芥AtICEs为模型,推测茶树CsICE43蛋白与HOS1、MYB15、DREB1/2存在潜在的互作关系。亚细胞定位试验表明CsICE43定位于细胞核,与跨膜结构分析结果一致。综上所述,本研究发现CsICE43基因可能与茶树低温响应关联,为深入挖掘其基因功能与抗寒分子机理提供了一定的理论基础。
基金the Open Fund(PLC201104)of State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation (Chengdu University of Technology)the National Natural Science Foundation of China(No.61072073)the Key Project of Education Commission of Sichuan Province(No.10ZA072)
文摘Although the phase-shift seismic processing method has characteristics of high accuracy, good stability, high efficiency, and high-dip imaging, it is not able to adapt to strong lateral velocity variation. To overcome this defect, a finite-difference method in the frequency-space domain is introduced in the migration process, because it can adapt to strong lateral velocity variation and the coefficient is optimized by a hybrid genetic and simulated annealing algorithm. The two measures improve the precision of the approximation dispersion equation. Thus, the imaging effect is improved for areas of high-dip structure and strong lateral velocity variation. The migration imaging of a 2-D SEG/EAGE salt dome model proves that a better imaging effect in these areas is achieved by optimized phase-shift migration operator plus a finite-difference method based on a hybrid genetic and simulated annealing algorithm. The method proposed in this paper is better than conventional methods in imaging of areas of high-dip angle and strong lateral velocity variation.
基金supported by the National Key R&D Program of China (No.2018YFB1801003)the National Natural Science Foundation of China (Nos.61525501 and 61827817)+1 种基金the Beijing Natural Science Foundation (No.4192022)the Project of Shandong Province Higher Educational Science and Technology Program (No.J17KA089)。
文摘A tunable frequency-multiplying optoelectronic oscillator(OEO) based on a dual-parallel Mach-Zehnder modulator(DPMZM) is proposed and experimentally demonstrated. In the proposed system, the tunable fundamental microware signal is generated by a tunable optoelectronic oscillator incorporating a phase-shifted fiber Bragg grating(PS-FBG). By adjusting the DC bias of the DPMZM, the frequency-doubled microwave signal with a tunable frequency range from 11 GHz to 20 GHz and the frequency-quadrupled microwave signal with a tunable frequency range from 22.5 GHz to 26 GHz are generated. The phase noises of the fundamental, frequency-doubled and frequency-quadrupled signals at 10 k Hz offset frequency are-105.9 d Bc/Hz,-103.3 d Bc/Hz and-86.2 d Bc/Hz, respectively.
文摘为探究ICE(inducer of CBF expression)转录因子在高丛蓝莓花果发育进程中可能的调控机制,通过对VcICE基因家族进行全基因组鉴定及生物信息学分析,采用实时荧光定量PCR(qRT-PCR)技术分析VcICE基因亚家族成员在蓝莓花芽膨大和果实发育进程中的相对表达水平.结果表明:在高丛蓝莓‘Draper’基因组中鉴定出8个VcICE成员,系统进化分析将其划分为2个亚家族,各亚家族成员均包含S-rich,bHLH和ACT等保守结构域;VcICE基因启动子区域富含光响应、植物激素、生长发育及非生物胁迫相关顺式作用元件;VcICE亚家族基因在高丛蓝莓‘O’Neal’和‘Bluerain’花芽膨大与果实发育进程中的相对表达差异显著.研究结果以期为深入了解VcICE转录因子在蓝莓花果发育进程中的功能提供理论参考.
基金supported by the Scientific Fund for Chinese Universities (Grant No. BUPT 2009RC0413)the National "863" High Technology Projects (Grant No. 2009AA01Z224)
文摘Phase and amplitude regeneration are necessary for degraded differential phase-shift keying communication sys- tems. This paper proposes a regenerator based on semiconductor optical amplifier for differential phase-shift keying signals. The key regeneration mechanism is theoretically analysed. The effectiveness of semiconductor optical amplifier based regenerator is demonstrated by comparing the bit error rate and eye diagrams before and after regeneration for 40-Cbit/s differential phase-shift keying 1080-km transmission systems. The results show that regeneration effects are very well. Bit error rate is tess than 10-12 with the regenerator.
基金The project supported by the National Natural Science Foundation of China under Grant No. 10574126.
文摘We present a scheme to realize geometric phase-shift gate for two superconducting quantum interference device （SQUID） qubits coupled to a single-mode microwave field. The geometric phase-shift gate operation is performed in two lower flux states, and the excited state [2〉 would not participate in the procedure. The SQUIDs undergo no transitions during the gate operation. Thus, the docoherence due to energy spontaneous emission based on the levels of SQUIDs are suppressed. The gate is insensitive to the cavity decay throughout the operation since the cavity mode is displaced along a circle in the phase space, acquiring a phase conditional upon the two lower flux states of the SQUID qubits, and the cavity mode is still in the original vacuum state. Based on the SQUID qubits interacting with the cavity mode, our proposed approach may open promising prospects for quantum iogic in SQUID-system.
文摘Broad-band all-optical wavelength conversion of differential phase-shift keyed （DPSK） signal is experimentally demonstrated. This scheme is composed of a one-bit delay interferometer demodulation stage followed by a semiconductor optical amplifier （SOA） based nonlinear polarization switch. A wavelength converter for the 10 G b/s DPSK signal is presented, which has a wide wavelength range of more than 30 nm. The converted signals experience small power penalties less than 1.4 dB compared with the original signal, at a bit error rate of 10-9. Additionally, the optical spectra, the measured waveforms and the open eye diagrams of the converted signals show a high quality wavelength conversion performance.
基金This work was supported in part by the Natural Science Foundation of Jiangsu Province under Grant BK20200969(L.Z.,URL:http://std.jiangsu.gov.cn/)in part by the Natural Science Foundation for Universities of Jiangsu Province under Grant 20KJB520008(Y.Y.,URL:http://jyt.jiangsu.gov.cn/)+2 种基金in part by the Nantong Science and Technology Plan Project under Grant JC2020148(Y.Y.,URL:http://kjj.nantong.gov.cn/)JC2020151(Y.C.,URL:http://kjj.nantong.gov.cn/)JC2019095(L.R.,URL:http://kjj.nantong.gov.cn/).
文摘focus of all countries.As an effective new energy,the fuel cell has attracted the attention of scholars.However,due to the particularity of proton exchange membrane fuel cell(PEMFC),the performance of traditional PI controlled phase-shifted full-bridge power electronics DC-DC converter cannot meet the needs of practical application.In order to further improve the dynamic performance of the converter,this paper first introduces several main topologies of the current mainstream front-end DC-DC converter,and analyzes their performance in the fuel cell system.Then,the operation process of the phase-shifted fullbridge power electronics DC-DC converter is introduced,and the shortcomings of the traditional PI control are analyzed.Finally,a double closed-loop adaptive fuzzy PI controller is proposed,which is characterized by dynamically adjusting PI parameters according to different working states to complete the intelligent control of phase-shifted full-bridge DC-DC converter.The simulation results in MATLAB/Simulink show that the proposed algorithm has good a control effect.Compared with the traditional algorithm,the overshoot and stabilization time of the system are shorter.The algorithm can effectively suppress the fluctuation of the output current of the fuel cell converter,and is a very practical control method.
基金Supported by the National Basic Research Program of China under Grant No 2013CB338002the National Natural Science Foundation of China under Grant Nos 11304397 and 61505261
文摘Recently, a novel kind of quantum key distribution called the round-robin differential phase-shift （RRDPS） protocol was proposed, which bounds the amount of leakage without monitoring signal disturbance. The protocol can be implemented by a weak coherent source. The security of this protocol with a simply characterized source has been proved. The application of a common phase shift can improve the secret key rate of the protocol. In practice, the randomized phase is discrete and the secret key rate is deviated from the continuous case. In this study, we analyze security of the RRDPS protocol with discrete-phase-randomized coherent state source and bound the secret key rate. We fix the length of each packet at 32 and 64, then simulate the secret key rates of the RRDPS protocol with discrete-phase randomization and continuous-phase randomization. Our simulation results show that the performance of the discrete-phase randomization case is close to the continuous counterpart with only a small number of discrete phases. The research is practically valuable for experimental implementation.
