The highly pathogenic avian influenza （HPAI） H5N1 virus has caused several outbreaks in domestic poultry. Despite great efforts to control the spread of this virus, it continues to evolve and poses a substantial thr...The highly pathogenic avian influenza （HPAI） H5N1 virus has caused several outbreaks in domestic poultry. Despite great efforts to control the spread of this virus, it continues to evolve and poses a substantial threat to public health because of a high mortality rate. In this study, we sequenced whole genomes of eight H5N1 avian influenza viruses isolated from domestic poultry in eastern China and compared them with those of typical influenza virus strains. Phylogenetic analyses showed that all eight genomes belonged to clade 2.3.2.1 and clade 7.2, the two main circulating clades in China. Viruses that clustered in clade 2.3.2.1 shared a high degree of homology with H5N1 isolates located in eastern Asian. Isolates that clustered in clade 7.2 were found to circulate throughout China, with an east-to-west density gradient. Pathogenicity studies in mice showed that these isolates replicate in the lungs, and clade 2.3.2.1 viruses exhibit a notably higher degree of virulence compared to clade 7.2 viruses. Our results contribute to the elucidation of the biological characterization and pathogenicity of HPAI H5N1 viruses.展开更多
Cotton disea ses represent a major challenge to cotton growth.Cloning of a cotton pathogen re-sponse gene and promoter is of great importance to improve disease resistance.In this study,a full length CC-NBS-LRRgene(GH...Cotton disea ses represent a major challenge to cotton growth.Cloning of a cotton pathogen re-sponse gene and promoter is of great importance to improve disease resistance.In this study,a full length CC-NBS-LRRgene(GHNBS)and its 5L flanking sequence have been cloned by race and tail PCR and further studied.The entire coding region is 2583 bp and enco des a polypeptide of 861 a mino acids with 28%maximum homology to an R gene of Arabi dopsis depo sited in the GenBank.Semi quantitativeRT-PCR showed thatGHNBS was expressed in floral buds,petals,phloem,root s,and leaves,and it has a greater exp ression pattern in roots and leaves.展开更多
基金supported in part by the funding from the National Natural Scientific Foundation(81370518)the National High Technology Research and Development Program of China(2015AA020924 and 2013ZX10004003)supported by a grant from the Beijing Nova Program(No.Z141107001814054)
文摘The highly pathogenic avian influenza （HPAI） H5N1 virus has caused several outbreaks in domestic poultry. Despite great efforts to control the spread of this virus, it continues to evolve and poses a substantial threat to public health because of a high mortality rate. In this study, we sequenced whole genomes of eight H5N1 avian influenza viruses isolated from domestic poultry in eastern China and compared them with those of typical influenza virus strains. Phylogenetic analyses showed that all eight genomes belonged to clade 2.3.2.1 and clade 7.2, the two main circulating clades in China. Viruses that clustered in clade 2.3.2.1 shared a high degree of homology with H5N1 isolates located in eastern Asian. Isolates that clustered in clade 7.2 were found to circulate throughout China, with an east-to-west density gradient. Pathogenicity studies in mice showed that these isolates replicate in the lungs, and clade 2.3.2.1 viruses exhibit a notably higher degree of virulence compared to clade 7.2 viruses. Our results contribute to the elucidation of the biological characterization and pathogenicity of HPAI H5N1 viruses.
文摘Cotton disea ses represent a major challenge to cotton growth.Cloning of a cotton pathogen re-sponse gene and promoter is of great importance to improve disease resistance.In this study,a full length CC-NBS-LRRgene(GHNBS)and its 5L flanking sequence have been cloned by race and tail PCR and further studied.The entire coding region is 2583 bp and enco des a polypeptide of 861 a mino acids with 28%maximum homology to an R gene of Arabi dopsis depo sited in the GenBank.Semi quantitativeRT-PCR showed thatGHNBS was expressed in floral buds,petals,phloem,root s,and leaves,and it has a greater exp ression pattern in roots and leaves.
