Spermatozoa have a highly complex RNA profile.Several of these transcripts are suggested as biomarkers for male infertility and contribute to early development.To analyze the differences between sperm RNA quantity and...Spermatozoa have a highly complex RNA profile.Several of these transcripts are suggested as biomarkers for male infertility and contribute to early development.To analyze the differences between sperm RNA quantity and expression of protamine(PRM1 and PRM2)and testis-specific histone 2B(TH2B)genes,spermatozoa from 33 patients who enrolled in assisted reproduction treatment(ART)program were analyzed.Sperm RNA of teratozoospermic(T),oligoteratozoospermic(OT),and normozoospermic(N)samples was extracted,and the differences in transcript levels among the study groups were analyzed by quantitative real-time polymerase chain reaction(qRT-PCR).The correlations of total RNA per spermatozoon and the expression of the transcripts were evaluated in relation to sperm characteristics and preimplantation embryo development.The mean(±standard deviation)RNA amount per spermatozoon was 28.48(±23.03)femtogram in the overall group and was significantly higher in the OT group than that in N and T groups.Total sperm RNA and gene expression of PRM1 and PRM2 genes were related to preimplantation embryo development and developmental arrest.Specific sperm characteristics were correlated with the expressions of PRM1,PRM2,or TH2B genes.We conclude that the sperm RNA amount and composition are important factors and might influence early embryonic development and also differ in different cases of male infertility.展开更多
目的探讨少弱精子症患者与正常人精液精子中PRM2基因m RNA的表达水平。方法选取少弱精子症患者20例为实验组,精子正常者10例为对照组,比较两组间精子浓度、活力和PRM2 m RNA水平的变化。结果 PRM2 m RNA水平,实验组与对照组比较差异有...目的探讨少弱精子症患者与正常人精液精子中PRM2基因m RNA的表达水平。方法选取少弱精子症患者20例为实验组,精子正常者10例为对照组,比较两组间精子浓度、活力和PRM2 m RNA水平的变化。结果 PRM2 m RNA水平,实验组与对照组比较差异有统计学意义(P<0.05);精子浓度,两组间差异有显著统计学意义(P<0.01);A级精子,两组间比较差异有显著统计学意义(P<0.01);A+B级精子,两组间差异有显著统计学意义(P<0.01);另外PRM2 m RNA水平与A级精子和A+B级之间存在正相关性(相关系数分别为0.419,0.571;P值分别为0.021,0.001)。结论少弱精子症患者其PRM2基因m RNA表达水平低于正常人,我们推测PRM2基因的m RNA的表达水平可以在一定程度上反映患者的精子质量水平,检测精子中PRM2 m RNA的表达水平能在一定程度上反映睾丸精子发生过程中的表达事件,可成为男性不育症病因研究、检测和临床治疗效果观察的一个重要指标。展开更多
基金supported by the Scientific Research Projects Coordination Unit of Istanbul University(No.13930).
文摘Spermatozoa have a highly complex RNA profile.Several of these transcripts are suggested as biomarkers for male infertility and contribute to early development.To analyze the differences between sperm RNA quantity and expression of protamine(PRM1 and PRM2)and testis-specific histone 2B(TH2B)genes,spermatozoa from 33 patients who enrolled in assisted reproduction treatment(ART)program were analyzed.Sperm RNA of teratozoospermic(T),oligoteratozoospermic(OT),and normozoospermic(N)samples was extracted,and the differences in transcript levels among the study groups were analyzed by quantitative real-time polymerase chain reaction(qRT-PCR).The correlations of total RNA per spermatozoon and the expression of the transcripts were evaluated in relation to sperm characteristics and preimplantation embryo development.The mean(±standard deviation)RNA amount per spermatozoon was 28.48(±23.03)femtogram in the overall group and was significantly higher in the OT group than that in N and T groups.Total sperm RNA and gene expression of PRM1 and PRM2 genes were related to preimplantation embryo development and developmental arrest.Specific sperm characteristics were correlated with the expressions of PRM1,PRM2,or TH2B genes.We conclude that the sperm RNA amount and composition are important factors and might influence early embryonic development and also differ in different cases of male infertility.
文摘目的探讨少弱精子症患者与正常人精液精子中PRM2基因m RNA的表达水平。方法选取少弱精子症患者20例为实验组,精子正常者10例为对照组,比较两组间精子浓度、活力和PRM2 m RNA水平的变化。结果 PRM2 m RNA水平,实验组与对照组比较差异有统计学意义(P<0.05);精子浓度,两组间差异有显著统计学意义(P<0.01);A级精子,两组间比较差异有显著统计学意义(P<0.01);A+B级精子,两组间差异有显著统计学意义(P<0.01);另外PRM2 m RNA水平与A级精子和A+B级之间存在正相关性(相关系数分别为0.419,0.571;P值分别为0.021,0.001)。结论少弱精子症患者其PRM2基因m RNA表达水平低于正常人,我们推测PRM2基因的m RNA的表达水平可以在一定程度上反映患者的精子质量水平,检测精子中PRM2 m RNA的表达水平能在一定程度上反映睾丸精子发生过程中的表达事件,可成为男性不育症病因研究、检测和临床治疗效果观察的一个重要指标。
