The purpose of this paper is to develop an analytic way for designing optimal PI-controllers for the interval plant family. Optimality means that the coefficient intervals of the plant stabilized by a PI-controller is...The purpose of this paper is to develop an analytic way for designing optimal PI-controllers for the interval plant family. Optimality means that the coefficient intervals of the plant stabilized by a PI-controller is maximized. It will be shown that the optimization problem can be formulated in terms of an eigenvalue minimization problem of matrix pairs of the form （H（h0, g0）, H（δ1,k, δ2,k））, where k= 1, 2, 3, 4 and both H（h0, g0） and H（δ1,k,δ2,k） are frequency independent Hurwitz-like matrices. A numerical example is provided to illustrate that optimal controller parameters can be successfully obtained by a two-dimensional search.展开更多
基金the National Natural Science Foundation of China (No.69904003)the Research Fund for Doctoral Program of the Higher Education (RFDP) (No.1999000701.)
文摘The purpose of this paper is to develop an analytic way for designing optimal PI-controllers for the interval plant family. Optimality means that the coefficient intervals of the plant stabilized by a PI-controller is maximized. It will be shown that the optimization problem can be formulated in terms of an eigenvalue minimization problem of matrix pairs of the form （H（h0, g0）, H（δ1,k, δ2,k））, where k= 1, 2, 3, 4 and both H（h0, g0） and H（δ1,k,δ2,k） are frequency independent Hurwitz-like matrices. A numerical example is provided to illustrate that optimal controller parameters can be successfully obtained by a two-dimensional search.
文摘为了建立基于全柱成像毛细管等电聚焦电泳(capillary isoelectric focusing,cIEF)技术的重组呼吸道合胞病毒蛋白疫苗(CHO细胞)原液抗原等电点(isoelectric point,pI)检测方法,完善疫苗质量控制体系,本研究根据抗原结构特性,采用变性剂RapiGest SF及还原剂二硫苏糖醇对蛋白进行前处理,确保其稳定单体化。通过优化蛋白助溶剂种类、添加比例及供试品上样量等关键参数,建立高效、稳定的cIEF检测方法,并对其主要验证参数和耐用性进行评价。优化后检测条件为使用含34%SimpleSol Protein Solubilizer、0.35%甲基纤维素(methylcellulose,MC)、4%两性电解质及1%等电点标记物混合溶液的样品缓冲液,聚焦程序设定为:1500 V 1 min,3000 V 7 min。采用该方法分析重组呼吸道合胞病毒蛋白疫苗(CHO细胞)原液抗原等电点,可得到峰型清楚且分离度高的图谱。本研究成功建立了适用于重组呼吸道合胞病毒蛋白疫苗(CHO细胞)原液抗原pI检测的cIEF方法。该方法可为该疫苗的分子表征及生产过程中的质量控制提供可靠的技术支持,并为同类重组蛋白疫苗的研发与标准化分析提供参考依据。
