以沈阳地铁公安无线通信系统为研究对象,针对既有系统构成复杂、设备老化和急需制式更新等问题,文章提出了一种从泛欧集群无线电(Trans European Trunked Radio,TETRA)制式转入警用数字集群制式的平滑转网方案,分别从公安无线通信系统...以沈阳地铁公安无线通信系统为研究对象,针对既有系统构成复杂、设备老化和急需制式更新等问题,文章提出了一种从泛欧集群无线电(Trans European Trunked Radio,TETRA)制式转入警用数字集群制式的平滑转网方案,分别从公安无线通信系统交换控制中心、无线网、传输链路和业务平台方面进行详细介绍,得出系统应采用兼容PDT与TETRA制式混合组网模式进行平滑过渡,逐步实现PDT制式转网的结论,可为类似项目提供借鉴和实施经验。展开更多
Photodynamic therapy(PDT),as a non-invasive treatment,is expected to be widely used in clinical cancer treatment.In this paper,a near-infrared(NIR)light responsive Lu_(2)O_(3):Yb/Er/Li-Ce6@MIL-101/GOx-PDA(LCMGP)nanodi...Photodynamic therapy(PDT),as a non-invasive treatment,is expected to be widely used in clinical cancer treatment.In this paper,a near-infrared(NIR)light responsive Lu_(2)O_(3):Yb/Er/Li-Ce6@MIL-101/GOx-PDA(LCMGP)nanodiagnostic platform was prepared for CT/PA imaging-guided multimodality synergistic therapy.Under 808 nm laser irradiation,Lu_(2)O_(3):Yb/Er/Li-Ce6(LC)NPs in the nano-diagnostic platform are activated and sensitized by NIR light,resulting in the generation of more single-linear oxygen(^(1)O_(2))for efficient PDT.Glucose oxidase(GOx)and MIL-101(Fe)nanozymes can utilize their excellent enzyme cascade catalytic properties and peroxidase-like catalytic properties to undergo glycolysis reactions and catalyze the decomposition of endogenous hydrogen peroxide(H_(2)O_(2)),producing a large amount of hydroxyl radicals(·OH),achieving efficient chemodynamic therapy(CDT).The functionalized modification of polydopamine(PDA)endows the LCMGP nanoparticles with higher photothermal conversion efficiency,which can prompt high local temperature at the tumor site under the irradiation of NIR light to achieve efficient photothermal therapy(PTT).In addition,hyperthermia accelerates the catalytic efficiency of the nanozymatic cascade of LCMGP nanoparticles to enhance ROS production while increasing cellular uptake,resulting in PTT-induced PDT/CDT synergistic effect(96%).Based on the excellent photothermal conversion effect of PDA and the high X-ray atte nuation effect of Lu/Fe,the LCMGP nanotherapeutic system demonstrates good CT/PA imaging function,providing new insights and ideas for imaging-mediated multimodal synergistic therapy.展开更多
目的：观察PDT联合玻璃体腔注射ranibizumab（雷珠单抗）治疗老年性黄斑变性脉络膜新生血管（choroidal neovascularization,CNV）的疗效。方法：将符合纳入标准,经吲哚青绿脉络膜血管造影（indocyanine green angiography,ICGA）、光...目的：观察PDT联合玻璃体腔注射ranibizumab（雷珠单抗）治疗老年性黄斑变性脉络膜新生血管（choroidal neovascularization,CNV）的疗效。方法：将符合纳入标准,经吲哚青绿脉络膜血管造影（indocyanine green angiography,ICGA）、光学相干断层扫描（optical coherence tomography,OCT）检查确诊为黄斑区脉络膜新生血管（CNV）患者27例27眼,经PDT治疗后3~7d内行ranibizumab玻璃体腔注射。观察治疗后1,3,6mo、末次随访时行最佳矫正视力、FFA、ICGA、OCT检查及有无并发症发生情况。结果：最佳矫正视力提高17眼（63%）,最佳矫正视力稳定6眼（22%）,最佳矫正视力下降4眼（15%）。27例27眼治疗前平均渗漏面积为1 005.69±105.47μm,治疗后1,3mo后平均875.54±103.27,423.37±79.68μm,与治疗前比较差异有统计学意义（P〈0.01）,视网膜黄斑中央厚度27例27眼治疗前平均厚度为485.58±122.59μm,治疗后1,3mo后平均398.84±105.32,297.74±89.18μm,与治疗前比较差异有统计学意义（P〈0.01）。结论：PDT封闭CNV后,联合玻璃体内腔内注射ranibizumab,有效阻断新生血管复发,减少PDT再次治疗次数和并发症,可提高治疗效果。展开更多
文摘以沈阳地铁公安无线通信系统为研究对象,针对既有系统构成复杂、设备老化和急需制式更新等问题,文章提出了一种从泛欧集群无线电(Trans European Trunked Radio,TETRA)制式转入警用数字集群制式的平滑转网方案,分别从公安无线通信系统交换控制中心、无线网、传输链路和业务平台方面进行详细介绍,得出系统应采用兼容PDT与TETRA制式混合组网模式进行平滑过渡,逐步实现PDT制式转网的结论,可为类似项目提供借鉴和实施经验。
基金Project supported by the Natural Science Foundation of Shandong Province(ZR2020ME045,ZR2020ME046)the Taishan Scholar Project of Shandong Province(tsqn201812130)+2 种基金the National Natural Science Foundation of China(82272833)"New Universities 20"Foundation of Jinan(2021GXRC099,T202204)China Postdoctoral Science Foundation(2022M711438)。
文摘Photodynamic therapy(PDT),as a non-invasive treatment,is expected to be widely used in clinical cancer treatment.In this paper,a near-infrared(NIR)light responsive Lu_(2)O_(3):Yb/Er/Li-Ce6@MIL-101/GOx-PDA(LCMGP)nanodiagnostic platform was prepared for CT/PA imaging-guided multimodality synergistic therapy.Under 808 nm laser irradiation,Lu_(2)O_(3):Yb/Er/Li-Ce6(LC)NPs in the nano-diagnostic platform are activated and sensitized by NIR light,resulting in the generation of more single-linear oxygen(^(1)O_(2))for efficient PDT.Glucose oxidase(GOx)and MIL-101(Fe)nanozymes can utilize their excellent enzyme cascade catalytic properties and peroxidase-like catalytic properties to undergo glycolysis reactions and catalyze the decomposition of endogenous hydrogen peroxide(H_(2)O_(2)),producing a large amount of hydroxyl radicals(·OH),achieving efficient chemodynamic therapy(CDT).The functionalized modification of polydopamine(PDA)endows the LCMGP nanoparticles with higher photothermal conversion efficiency,which can prompt high local temperature at the tumor site under the irradiation of NIR light to achieve efficient photothermal therapy(PTT).In addition,hyperthermia accelerates the catalytic efficiency of the nanozymatic cascade of LCMGP nanoparticles to enhance ROS production while increasing cellular uptake,resulting in PTT-induced PDT/CDT synergistic effect(96%).Based on the excellent photothermal conversion effect of PDA and the high X-ray atte nuation effect of Lu/Fe,the LCMGP nanotherapeutic system demonstrates good CT/PA imaging function,providing new insights and ideas for imaging-mediated multimodal synergistic therapy.
文摘目的:检测5-氨基酮戊酸-光动力疗法(ALA-PDT)治疗前后尖锐湿疣皮损凋亡相关因子(Fas、caspase3、Bcl-2)及促炎症细胞因子(CCL-20、TNF-α、IL-1)的表达,探讨AL A-PDT治疗尖锐湿疣的可能机理。方法:收集10例尖锐湿疣患者ALA-PDT治疗前后皮损(同时以正常健康者皮肤组织对照),采用荧光定量PCR检测其Fas、caspase3、Bcl-2、CCL-20、TNF-α、IL-1 mRNA表达;Western blot检测其Fas、caspase3、Bcl-2、CCL-20、TNF-α、IL-1蛋白表达。结果:尖锐湿疣皮损组织内Fas、caspase3、Bcl-2、CCL-20、TNF-α、IL-1 mRNA和蛋白表达均高于正常皮肤组织(P<0.05);与治疗前比较,治疗后Bcl-2、CCL-20、TNF-α、IL-1 m RNA和蛋白表达水平明显降低(P<0.01),Fas、caspase3 m RNA和蛋白表达水平明显升高(P<0.01)。结论:AL A-PDT是一种能有效治疗尖锐湿疣的方法,它可诱导细胞表面凋亡蛋白Fas、caspase3的表达、减少凋亡抑制因子Bcl-2的表达,从而可能依靠内源性途径(线粒体介导的凋亡)和外源性途径(死亡受体介导的凋亡)促进尖锐湿疣角质形成细胞凋亡;并可能通过抑制促炎细胞因子CCL-20、TNF-α、IL-1而发挥局部免疫调节作用。
