本研究提取中国东北汉族和达斡尔、鄂伦春、鄂温克等 4个人群的基因组 DNA后 ,利用PCR- RFLP方法扩增 apo E基因第 4外显子片段并进行遗传分析 ,计算各个人群中的 apo E等位基因频率。结果发现 4个人群中的 apo E等位基因频率分布趋势...本研究提取中国东北汉族和达斡尔、鄂伦春、鄂温克等 4个人群的基因组 DNA后 ,利用PCR- RFLP方法扩增 apo E基因第 4外显子片段并进行遗传分析 ,计算各个人群中的 apo E等位基因频率。结果发现 4个人群中的 apo E等位基因频率分布趋势与国内外报告基本一致 ,但等位基因ε3展开更多
To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to...To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to am plify the gene and Eco RΙ and Hind were used to generate the RFL P fingerprinting.Target DNA fragm ents from 13of2 0 samples were successfully amplified and the relevant RFL P fingerprintings were obtained.It is concluded thatthe m ethod can be used to amplify the whole Cag A gene and Cag A gene has apparent diversity of RFL P profile.展开更多
基金Thisprojectwassupported by a grant from the MinistryofPublicHealth(Serial No.:98- 1- 12 3)
文摘To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to am plify the gene and Eco RΙ and Hind were used to generate the RFL P fingerprinting.Target DNA fragm ents from 13of2 0 samples were successfully amplified and the relevant RFL P fingerprintings were obtained.It is concluded thatthe m ethod can be used to amplify the whole Cag A gene and Cag A gene has apparent diversity of RFL P profile.
