Background:Benign prostatic hyperplasia(BPH)is the most common disease in elderly men.There is increasing evidence that periodontitis increases the risk of BPH,but the specific mechanism remains unclear.This study aim...Background:Benign prostatic hyperplasia(BPH)is the most common disease in elderly men.There is increasing evidence that periodontitis increases the risk of BPH,but the specific mechanism remains unclear.This study aimed to explore the role and mechanism of the key periodontal pathogen Porphyromonas gingivalis(P.gingivalis)in the development of BPH.Methods:The subgingival plaque(Sp)and prostatic fluid(Pf)of patients with BPH concurrent periodontitis were extracted and cultured for 16S r DNA sequencing.Ligature-induced periodontitis,testosterone-induced BPH and the composite models in rats were established.The P.gingivalis and its toxic factor P.gingivalis lipopolysaccharide(P.gLPS)were injected into the ventral lobe of prostate in rats to simulate its colonization of prostate.P.g-LPS was used to construct the prostate cell infection model for mechanism exploration.Results:P.gingivalis,Streptococcus oralis,Capnocytophaga ochracea and other oral pathogens were simultaneously detected in the Sp and Pf of patients with BPH concurrent periodontitis,and the average relative abundance of P.gingivalis was found to be the highest.P.gingivalis was detected in both Sp and Pf in 62.5%of patients.Simultaneous periodontitis and BPH synergistically aggravated prostate histological changes.P.gingivalis and P.gLPS infection could induce obvious hyperplasia of the prostate epithelium and stroma(epithelial thickness was 2.97-fold and 3.08-fold that of control group,respectively),and increase of collagen fibrosis(3.81-fold and 5.02-fold that of control group,respectively).P.gingivalis infection promoted prostate cell proliferation,inhibited apoptosis,and upregulated the expression of inflammatory cytokines interleukin-6(IL-6;4.47-fold),interleukin-6 receptor-α(IL-6Rα;5.74-fold)and glycoprotein 130(gp130;4.47-fold)in prostatic tissue.P.g-LPS could significantly inhibit cell apoptosis,promote mitosis and proliferation of cells.P.g-LPS activates the Akt pathway through IL-6/IL-6Rα/gp130 complex,which destroys the imbalance between proliferation and apoptosis of prostate cells,induces BPH.Conclusion:P.gingivalis was abundant in the Pf of patients with BPH concurrent periodontitis.P.gingivalis infection can promote BPH,which may affect the progression of BPH via inflammation and the Akt signaling pathway.展开更多
针对耦合神经P系统利用脉冲机制实现区域生长依赖于初始种子点选择的问题,提出一种自适应区域生长耦合神经P系统(adaptive region growing coupled neural P systems,ARGCNP)的图像分割方法。该方法利用金豺优化算法(golden jackal opti...针对耦合神经P系统利用脉冲机制实现区域生长依赖于初始种子点选择的问题,提出一种自适应区域生长耦合神经P系统(adaptive region growing coupled neural P systems,ARGCNP)的图像分割方法。该方法利用金豺优化算法(golden jackal optimization,GJO)的全局搜索能力,通过引入四种策略提升GJO的全局寻优性能,从而在图像中寻找最佳阈值点,以优化区域生长中的种子点选择。在实验中,首先通过CEC2017测试函数对改进后的GJO进行性能测试,结果表明改进后的GJO在测试函数上整体性能第一;随后将ARGCNP应用于分割彩色图像和医学图像,以峰值信噪比等三个指标对分割效果进行量化评价,分割结果显示该方法能够提升分割精度及分割结果的稳定性,证明ARGCNP在应用场景下具有的优势,能够满足图像分割需求。展开更多
为了明晰人类活动对冶木河流域水文情势的影响效果,基于冶力关水文站1981—2020年的年径流量和日均流量数据,采用曼宁肯德尔-彭曼(Mann-Kendall-Pettitt,MK-P)突变点检测、水文指标(Indicators of Hydrologic Alteration,IHA)和动态时...为了明晰人类活动对冶木河流域水文情势的影响效果,基于冶力关水文站1981—2020年的年径流量和日均流量数据,采用曼宁肯德尔-彭曼(Mann-Kendall-Pettitt,MK-P)突变点检测、水文指标(Indicators of Hydrologic Alteration,IHA)和动态时间弯曲(Dynamic Time Warping,DTW)算法计算了基准期和黄河流域生态保护和高质量发展重大国家战略实施影响期的水文指标变化情况及两个时期的水文情势相似度。基于全球陆面数据同化系统(Global Land Data Assimilation System,GLDAS)提取冶木河陆地水储量,分析其与径流量的相关关系及变化时间点。研究结果表明:1)冶木河径流量平稳变化点和陆地水储量变化点均为2018年。2)战略实施影响期的径流量大于基准期,径流量平均增大29.79%。3)基准期和战略实施影响期5组水文情势指标的相似度分别为0.025、0.025、0.25、0.1、0,说明冶木河流量极值出现的次数增加,小流量持续情况有所好转。4)战略实施影响期实施的水源涵养措施袭夺了河川基流,减水效果较明显。5)陆地水储量与径流量相关关系较弱。展开更多
基金supported(in part)by the National Natural Science Foundation of China(82200862,82370778)the Hubei Provincial Natural Science Foundation(2022CFB681,2023AFA061,2019CFB760)+4 种基金the Hubei Province Health and Family Planning Scientific Research Project(WJ2023M058,WJ2019H035)the Key Scientific Research Project of Education Department of Henan Province(22A320038)the Fundamental Research Funds for the Central Universities(2042023kf1019,2042023kf0051,2042022kf0072)the Zhongnan Hospital of Wuhan University,Science Technology and Innovation Seed Fund(CXPY2022074)the Young Top-notch Talent Cultivation Program of Hubei Province(for Prof.Zeng XT).
文摘Background:Benign prostatic hyperplasia(BPH)is the most common disease in elderly men.There is increasing evidence that periodontitis increases the risk of BPH,but the specific mechanism remains unclear.This study aimed to explore the role and mechanism of the key periodontal pathogen Porphyromonas gingivalis(P.gingivalis)in the development of BPH.Methods:The subgingival plaque(Sp)and prostatic fluid(Pf)of patients with BPH concurrent periodontitis were extracted and cultured for 16S r DNA sequencing.Ligature-induced periodontitis,testosterone-induced BPH and the composite models in rats were established.The P.gingivalis and its toxic factor P.gingivalis lipopolysaccharide(P.gLPS)were injected into the ventral lobe of prostate in rats to simulate its colonization of prostate.P.g-LPS was used to construct the prostate cell infection model for mechanism exploration.Results:P.gingivalis,Streptococcus oralis,Capnocytophaga ochracea and other oral pathogens were simultaneously detected in the Sp and Pf of patients with BPH concurrent periodontitis,and the average relative abundance of P.gingivalis was found to be the highest.P.gingivalis was detected in both Sp and Pf in 62.5%of patients.Simultaneous periodontitis and BPH synergistically aggravated prostate histological changes.P.gingivalis and P.gLPS infection could induce obvious hyperplasia of the prostate epithelium and stroma(epithelial thickness was 2.97-fold and 3.08-fold that of control group,respectively),and increase of collagen fibrosis(3.81-fold and 5.02-fold that of control group,respectively).P.gingivalis infection promoted prostate cell proliferation,inhibited apoptosis,and upregulated the expression of inflammatory cytokines interleukin-6(IL-6;4.47-fold),interleukin-6 receptor-α(IL-6Rα;5.74-fold)and glycoprotein 130(gp130;4.47-fold)in prostatic tissue.P.g-LPS could significantly inhibit cell apoptosis,promote mitosis and proliferation of cells.P.g-LPS activates the Akt pathway through IL-6/IL-6Rα/gp130 complex,which destroys the imbalance between proliferation and apoptosis of prostate cells,induces BPH.Conclusion:P.gingivalis was abundant in the Pf of patients with BPH concurrent periodontitis.P.gingivalis infection can promote BPH,which may affect the progression of BPH via inflammation and the Akt signaling pathway.
文摘针对耦合神经P系统利用脉冲机制实现区域生长依赖于初始种子点选择的问题,提出一种自适应区域生长耦合神经P系统(adaptive region growing coupled neural P systems,ARGCNP)的图像分割方法。该方法利用金豺优化算法(golden jackal optimization,GJO)的全局搜索能力,通过引入四种策略提升GJO的全局寻优性能,从而在图像中寻找最佳阈值点,以优化区域生长中的种子点选择。在实验中,首先通过CEC2017测试函数对改进后的GJO进行性能测试,结果表明改进后的GJO在测试函数上整体性能第一;随后将ARGCNP应用于分割彩色图像和医学图像,以峰值信噪比等三个指标对分割效果进行量化评价,分割结果显示该方法能够提升分割精度及分割结果的稳定性,证明ARGCNP在应用场景下具有的优势,能够满足图像分割需求。
文摘为了明晰人类活动对冶木河流域水文情势的影响效果,基于冶力关水文站1981—2020年的年径流量和日均流量数据,采用曼宁肯德尔-彭曼(Mann-Kendall-Pettitt,MK-P)突变点检测、水文指标(Indicators of Hydrologic Alteration,IHA)和动态时间弯曲(Dynamic Time Warping,DTW)算法计算了基准期和黄河流域生态保护和高质量发展重大国家战略实施影响期的水文指标变化情况及两个时期的水文情势相似度。基于全球陆面数据同化系统(Global Land Data Assimilation System,GLDAS)提取冶木河陆地水储量,分析其与径流量的相关关系及变化时间点。研究结果表明:1)冶木河径流量平稳变化点和陆地水储量变化点均为2018年。2)战略实施影响期的径流量大于基准期,径流量平均增大29.79%。3)基准期和战略实施影响期5组水文情势指标的相似度分别为0.025、0.025、0.25、0.1、0,说明冶木河流量极值出现的次数增加,小流量持续情况有所好转。4)战略实施影响期实施的水源涵养措施袭夺了河川基流,减水效果较明显。5)陆地水储量与径流量相关关系较弱。
