Mitochondrial genome has been widely used in species identification and gene conservation.In the present study,the complete mitochondrial genome of Oula sheep(Ovis aries)was determined using next-generation sequencing...Mitochondrial genome has been widely used in species identification and gene conservation.In the present study,the complete mitochondrial genome of Oula sheep(Ovis aries)was determined using next-generation sequencing.This genome was16618 bp(NCBI accession number:KU575248)and contained 13 protein coding genes,22 transfer RNA genes,two ribosomal RNA genes,and a typical control region.The overall nucleotide composition was 33.7%A,27.4%T,25.8%C,and 13.1%G,with a total A+T content of 61.1%.The phylogenetic analysis of selected sheep breeds showed that Oula sheep were clustered within branch A and originated from approximately 6 ka.This mitochondrial genome will provide valuable information for molecular genetic research of Oula sheep.展开更多
The Tibetan antelope(Pantholops hodgsonii),blue sheep(Pseudois nayaur),and Tibetan sheep(Ovis aries)are the dominant small ruminants in the Three-River-Source National Park(TRSNP).However,knowledge about the associati...The Tibetan antelope(Pantholops hodgsonii),blue sheep(Pseudois nayaur),and Tibetan sheep(Ovis aries)are the dominant small ruminants in the Three-River-Source National Park(TRSNP).However,knowledge about the association between gut microbiota and host adaptability remains poorly understood.Herein,multi-omics sequencing approaches were employed to investigate the gut microbiota-mediated forage adaption in these ruminants.The results revealed that although wild ruminants(WR)of P.hodgsoni and P.nayaur were faced with severe foraging environments with significantly low vegetation coverage and nutrition,the apparent forage digestibility of dry matter,crude protein,and acid detergent fiber was significantly higher than that of O.aries.The 16s rRNA sequencing showed that the gut microbiota in WR underwent convergent evolution,and alpha diversity in these two groups was significantly higher than that in O.aries.Moreover,indicator species,including Bacteroidetes and Firmicutes,exhibited positive relationships with apparent forage digestibility,and their relative abundances were enriched in the gut of WR.Enterotype analysis further revealed that enterotype 1 belonged to WR,and the abundance of fatty acid synthesis metabolic pathway-related enzyme genes was significantly higher than enterotype 2,represented by O.aries.Besides,the metagenomic analysis identified 14 pathogenic bacterial species,among which 10 potentially pathogenic bacteria were significantly enriched in the gut microbiota of O.aries.Furthermore,the cellulolytic strains and genes encoding cellulase and hemicellulase were significantly enriched in WR.In conclusion,our results provide new evidence of gut microbiota to facilitate wildlife adaption in severe foraging environments of the TRSNP,China.展开更多
基金Supported by the Central Public-Interest Scientific Institution Basal Research Fund(1610322016006)Science and Technology Support Projects in Gansu Province(1504NKCA052)the Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2014-LIHPS-01)
文摘Mitochondrial genome has been widely used in species identification and gene conservation.In the present study,the complete mitochondrial genome of Oula sheep(Ovis aries)was determined using next-generation sequencing.This genome was16618 bp(NCBI accession number:KU575248)and contained 13 protein coding genes,22 transfer RNA genes,two ribosomal RNA genes,and a typical control region.The overall nucleotide composition was 33.7%A,27.4%T,25.8%C,and 13.1%G,with a total A+T content of 61.1%.The phylogenetic analysis of selected sheep breeds showed that Oula sheep were clustered within branch A and originated from approximately 6 ka.This mitochondrial genome will provide valuable information for molecular genetic research of Oula sheep.
基金supported by the Youth Fund Project of the National Natural Science Foundation of China(32100100)the Joint Grant from the Chinese Academy of Sciences-People’s Government of Qinghai Province on Sanjiangyuan National Park(LHZX-2022-02)+1 种基金the Youth Innovation Promotion Association,Chinese Academy of Sciencesthe National Science Foundation of Qinghai Province(2022-ZJ-943Q).
文摘The Tibetan antelope(Pantholops hodgsonii),blue sheep(Pseudois nayaur),and Tibetan sheep(Ovis aries)are the dominant small ruminants in the Three-River-Source National Park(TRSNP).However,knowledge about the association between gut microbiota and host adaptability remains poorly understood.Herein,multi-omics sequencing approaches were employed to investigate the gut microbiota-mediated forage adaption in these ruminants.The results revealed that although wild ruminants(WR)of P.hodgsoni and P.nayaur were faced with severe foraging environments with significantly low vegetation coverage and nutrition,the apparent forage digestibility of dry matter,crude protein,and acid detergent fiber was significantly higher than that of O.aries.The 16s rRNA sequencing showed that the gut microbiota in WR underwent convergent evolution,and alpha diversity in these two groups was significantly higher than that in O.aries.Moreover,indicator species,including Bacteroidetes and Firmicutes,exhibited positive relationships with apparent forage digestibility,and their relative abundances were enriched in the gut of WR.Enterotype analysis further revealed that enterotype 1 belonged to WR,and the abundance of fatty acid synthesis metabolic pathway-related enzyme genes was significantly higher than enterotype 2,represented by O.aries.Besides,the metagenomic analysis identified 14 pathogenic bacterial species,among which 10 potentially pathogenic bacteria were significantly enriched in the gut microbiota of O.aries.Furthermore,the cellulolytic strains and genes encoding cellulase and hemicellulase were significantly enriched in WR.In conclusion,our results provide new evidence of gut microbiota to facilitate wildlife adaption in severe foraging environments of the TRSNP,China.
