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Ostrich breeder rides wave of online popularity
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作者 李霄梅 《疯狂英语(新读写)》 2025年第3期50-51,77,共3页
While feeding her ostriches,Wang Xue swiftly responds to fans'questions with a sense of humor through her popular live‑streaming channel.Since Wang,30,an expert in ostrich farming,started live broadcasting on a sh... While feeding her ostriches,Wang Xue swiftly responds to fans'questions with a sense of humor through her popular live‑streaming channel.Since Wang,30,an expert in ostrich farming,started live broadcasting on a short‑video platform with her husband Han Peng,their ostrich farming business has flourished,garnering nearly 3 million fans on the platform.“The highest viewership for one single live broadcast reached over 6 million,”said Wang,adding that riding an ostrich at high speed was an essential part of her broadcasts.Through her videos,Wang brought fans close to the ostriches,providing details of their habits,farming methods and economic value. 展开更多
关键词 live streaming ostrich live broadcast riding ostrich fan interaction HUMOR viewership POPULARITY
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Distribution Law of Goblet Cells in the Intestinal Tracts of African Ostrich Chicks 被引量:4
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作者 金春艳 彭克美 +4 位作者 刘婷婷 王蕾 曹维维 宋卉 刘华珍 《Agricultural Science & Technology》 CAS 2013年第1期103-106,共4页
[Objective] This study aimed to investigate the distribution law of different goblet cells in the intestinal tracts of African ostrich chicks. [Method] Alcian blue/pe- riodic acid-schiff reaction (AB/PAS) was adopte... [Objective] This study aimed to investigate the distribution law of different goblet cells in the intestinal tracts of African ostrich chicks. [Method] Alcian blue/pe- riodic acid-schiff reaction (AB/PAS) was adopted to observe and analyze the types and distribution of goblet cells in the intestinal tracts of ostrich chicks. Acid mu- copolysaccharide could be stained blue with alcian blue (AB), and neutral mu- copolysaccharide could be stained red with periodic acid-schiff reagent (PAS). [Result] According to AB/PAS results, goblet cells in the intestinal tracts were divided in- to four types: TypeⅠ was pure red, with AB negative result and PAS positive result containing neutral mucoitin; type Ⅱ was pure blue, with AB positive result and PAS negative result containing acidic mucoitin; type Ⅲ was purple reddish, with PAS posi- tive result greater than AB; typeⅣ was purple blue, with AB positive result greater than PAS. Large quantities of goblet cells were found in the intestinal tracts of os- trich chicks, mostly type III and IV.The quantities of goblet cells were decreased gradually in the duodenum, jejunum and ileum, while the quantities were increased in the cecum, colon and rectum. The goblet cells in the large intestine are more than that in the small intestine. The most quantities of goblet cells were contained in rectum. [Conclusion] These results indicate that the distribution of goblet cells is closely related with the structure and function of intestinal tracts. The mucus secret- ed by the goblet Cells plays a series of important roles in the digestion and mucosal immunization. 展开更多
关键词 AB/PAS ostrich chicks Goblet cell Mucosal immunization
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Morphological Differences in Africa Ostrich Chicks Suffering from Tibial Disease
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作者 王蕾 彭克美 +4 位作者 程佳月 陈敏 金春燕 刘婷婷 曹维维 《Agricultural Science & Technology》 CAS 2013年第2期336-339,360,共5页
[Objective] This study aimed to investigate the differences in morphological structure of tibias between sick and normal ostrich chicks. [Method] Normal and sick ostrich chicks aged 90 days were used in this research ... [Objective] This study aimed to investigate the differences in morphological structure of tibias between sick and normal ostrich chicks. [Method] Normal and sick ostrich chicks aged 90 days were used in this research for bone mineral density determination, bone demineralization paraffin section preparation (HE staining), bone abrasive disc preparation and scanning electron microscopy (SEM) observation, to analyze the differences in microstructure and ultrastructure of tibias between sick ostrich chicks and normal ones. [Result] Bone mineral density of ostrich chicks suffering from leg disease was much lower than that of the normal ones. Compared with normal tibias, less trabeculae were observed in sick tibias, which were attenuated and began to disappear. In addition, trabeculae micro-fracture could be observed under scanning electron microscope; resorption pits on bone slices formed by osteoclast could be observed under scanning electronic microscope. [Conclusion] These results indicate that the sick tibias all have lower bone density and trabeculae microfracture phenomenon in tibias with different diseases, which might be resulted from osteoporosis. 展开更多
关键词 Africa ostrich chick Tibia disease Morphology Differences
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Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered cornea 被引量:6
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作者 Xian-Ning Liu Xiu-Ping Zhu +10 位作者 Jie Wu Zheng-Jie Wu Yong Yin Xiang-HuaXiao Xin Su Bin Kong Shi-Yin Pan Hua Yang Yan Cheng Na An Sheng-Li Mi 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2016年第3期325-331,共7页
AIM: To assess acellular ostrich corneal matrix used as a scaffold to reconstruct a damaged cornea. METHODS: A hypertonic saline solution combined with a digestion method was used to decellularize the ostrich cornea... AIM: To assess acellular ostrich corneal matrix used as a scaffold to reconstruct a damaged cornea. METHODS: A hypertonic saline solution combined with a digestion method was used to decellularize the ostrich cornea. The microstructure of the acellular corneal matrix was observed by transmission electron microscopy (TEM) and hematoxylin and eosin (H&E) staining. The mechanical properties were detected by a rheometer and a tension machine. The acellular corneal matrix was also transplanted into a rabbit cornea and cytokeratin 3 was used to check the immune phenotype, RESULTS: The microstructure and mechanical properties of the ostrich cornea were well preserved after the decellularization process, in vitro, the methyl thiazolyl tetrazoUum results revealed that extracts of the acellular ostrich corneas (AOCs) had no inhibitory effects on the proliferation of the corneal epithelial or endothelial cells or on the keratocytes, The rabbit lamellar keratoplasty showed that the transplanted AOCs were transparent and completely incorporated into the host cornea while corneal turbidity and graft dissolution occurred in the acellular porcine cornea (APC) transplantation, The phenotype of the reconstructed cornea was similar to a normal rabbit cornea with a high expression of cytokeratin 3 in the superficial epithelial cell layer, CONCLUSION: We first used AOCs as scaffolds to reconstruct damaged corneas. Compared with porcine corneas, the anatomical structures of ostrich corneas are closer to those of human corneas. In accordance with the principle that structure determines function, a xenograft lamellar keratoplasty also confirmed that the AOC transplantation generated a superior outcome compared to that of the APC graft. 展开更多
关键词 ostrich acellular corneal stroma tissue engineering CORNEA
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Pathological Microstructure of a Miocene Ostrich Eggshell from Asia 被引量:2
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作者 WANG Shuo 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2007年第5期697-702,共6页
Struthio linxiaensis is the oldest fossil ostrich from Asia till today. This paper analyzes the microstructure of the eggshell of Struthio linxiaensis. The SEM and light microscopic results show that a pathological st... Struthio linxiaensis is the oldest fossil ostrich from Asia till today. This paper analyzes the microstructure of the eggshell of Struthio linxiaensis. The SEM and light microscopic results show that a pathological structure occurred associated with the formation of pore canal. The feature of this structure is that crystals of the columnar layer are around the lower part of the pore canal, and formed a broad structure. This structure unit contracts upwards more severely than the canal and looks like a "garlic", which was observed in aumiotes eggshells for the first time. Many evidences indicate that this structure was deposited with the columns simultaneously. The radial section view suggests that the "garlic structure" is not a result of antiperistaltic waves in the avian oviduct. The most possible process of the "garlic structure" formation is the column units around the pore canals with imperfect acidifying when calcified, or have some correlations with the changes in the protein profiles of the organic matrix in the calcareous eggshell. 展开更多
关键词 Struthio linxiaensis ostrich egg pathological structure MIOCENE Linxia Basin
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Protection against Infectious Bronchitis Virus, a Corona Virus Infection, Using Ostrich Antibodies 被引量:2
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作者 Yasuhiro Tsukamoto Yuna Nakano Kazuhide Adachi 《Health》 2018年第10期1294-1308,共15页
In chickens, infectious bronchitis (IB) is a major respiratory disease. The respiratory system is the primary multiplication site of IB virus (IBV), a coronavirus, after which the virus is distributed to other organs.... In chickens, infectious bronchitis (IB) is a major respiratory disease. The respiratory system is the primary multiplication site of IB virus (IBV), a coronavirus, after which the virus is distributed to other organs. Poultry farms sustain considerable economic damage due to IB outbreaks in flocks, since IB causes a severe reduction in weight gain in chicks. In the present study, we produced the ostrich IgY against IBV by immunizing female ostriches with the IB viral antigens. The resultant purified IgY showed a strong neutralizing activity against IBV infection of cultured primary chick kidney cells. The infectivity of IBV was markedly inhibited in the trachea of chicks when ostrich IgY was injected intra-muscularly into newly hatched chicks prior to viral inhalation challenge at two weeks of age. Furthermore, the infection was strongly blocked in the tracheae when IgY was injected into chicks at one day and one week of age, with viral inhalation performed at three weeks of age. These findings suggest that the injection of ostrich IgY can help protect young chicks from IBV infections. In south Asian and African countries, broiler chicks are sent to poultry market around 30 days of age, so it is important to prevent IB outbreaks in very young flocks. We strongly believe that ostrich IgY will be a powerful weapon against IB infection in poultry farms on a wide scale and also hope that these findings will aid in the development of antibody vaccines for new type corona viruses, SARS-CoV and MERS-CoV. 展开更多
关键词 ostrich Antibody INFECTIOUS BRONCHITIS CORONA Virus
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Ostrich Antibody and Its Application to Skin Diseases, a Review and Case Report 被引量:1
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作者 Yasuhiro Tsukamoto Osamu Maeda +2 位作者 Genshi Shigekawa Stuart Greenberg Barry Hendler 《Health》 2018年第10期1357-1370,共14页
Antibodies are immunoglobulins produced by B cells when antigens such as allergens or pathogens invade an animal’s body. The antibodies remove and inactivate antigens. Antibodies are distributed in internal body and ... Antibodies are immunoglobulins produced by B cells when antigens such as allergens or pathogens invade an animal’s body. The antibodies remove and inactivate antigens. Antibodies are distributed in internal body and mucosal membrane to protect living animals, but they are excellent proteins that can exert their functions, “antigen-antibody reactions,” even when removed from the body. For that reason, antibodies are being put to practical use in diagnostic kits for conditions such as pregnancy and influenza infection, and as anticancer drugs targeting specific tumor markers. The result has been an increasing use of antibodies for research, diagnosis, and therapeutic purposes. Unfortunately, antibodies from experimental mammals such as mice, rats, and rabbits, are not suited to industrial use because of their high production cost. Moreover, handling of these antibodies is difficult due to their vulnerability to heat, acids and alkalis. Accordingly, there is no adaptability to mass production. Recently, we developed a convenient method for the low-cost, mass-production of antibodies using egg-laying hen ostriches. The ostrich egg is an excellent source of antibodies for industrial purposes. The present report shows that the ostrich antibodies have therapeutic effects in ailments such as atopic dermatitis, acne, pyoderma, and pollen allergies. We have successfully produced and purified ostrich immunoglobulin yolk (IgY) against pollen allergens (Cryj1, Cryj2, Chao1, Chao2) and found that allergic reactions were alleviated in skin patch tests of allergic patients by using the ostrich IgY. In addition, we produced ostrich IgY against the homogenates of Staphylococcus aureus and Propionibacterium acnes, and applied to dermal lesions of atopic dermatitis and acne patients, and then observed the therapeutic effects on the dermatitis of volunteer subjects. Antibody against S. aureus also had the therapeutic effect on canine pyoderma caused by MRSA. A particular advantage in using ostrich antibodies is the fact that they inactivate and neutralize a specific antigen, without damaging the indigenous microflora of the dermal surface. In this review article and case repot, we wish to suggest that ostrich antibodies can contribute to the treatment of cutaneous disorders as an alternative to treatment with steroids or antibiotics. 展开更多
关键词 ostrich ANTIBODY Cosmetics ATOPIC DERMATITIS ACNE Pollen Allergy PYODERMA
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The Efficacy of Ostrich Antibodies to Dihydrotestosterone and 5<i>α</i>-Reductase in the Restoration of Hair Growth 被引量:1
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作者 Yasuhiro Tsukamoto Barry Hendler +1 位作者 Stuart Greenberg Carol Epstein 《Health》 2019年第10期1320-1330,共11页
The hormone dihydro testosterone (DHT) and the catalyst 5α-reductase are key contributors to hair loss due to androgenetic alopecia (AGA). Also, dermal bacteria and their toxins have been implicated in hair loss. A p... The hormone dihydro testosterone (DHT) and the catalyst 5α-reductase are key contributors to hair loss due to androgenetic alopecia (AGA). Also, dermal bacteria and their toxins have been implicated in hair loss. A preliminary study by the authors with six male volunteer subjects showed that ostrich antibodies against above causative substances were quite effective in promoting hair regrowth. The present study included 13 men and 4 women, with ages ranging from 39 to 78. Pre- and post-treatment hair counts were also incorporated into the study procedure. In all instances but one, the subjects had a hair-growth effect about 3 months after the antibody was applied to the scalp, and the study participants with hair growth were pleased with the results and with the ease of use of the antibody solution. 展开更多
关键词 ALOPECIA AGA ostrich Antibody
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Application of Ostrich Antibodies to the Restoration of Hair Growth, a Preliminary and Case Report 被引量:1
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作者 Yasuhiro Tsukamoto Barry Hendler Stuart Greenberg 《Journal of Cosmetics, Dermatological Sciences and Applications》 2018年第4期179-184,共6页
The hormone dihydrotestosterone (DHT) and the catalyst 5α-reductase play very important roles in the hair loss due to androgenetic alopecia (AGA). Recently, some chemicals against these hormones have been commerciali... The hormone dihydrotestosterone (DHT) and the catalyst 5α-reductase play very important roles in the hair loss due to androgenetic alopecia (AGA). Recently, some chemicals against these hormones have been commercialized as therapeutics for AGA. In the present study, we have attempted to find out the potential effects of antibodies on AGA therapeutics as an inhibitor of androgenic hormones. The immune system of the ostrich is believed to be the most versatile and powerful of any animal on the planet, and antibodies derived from the ostrich egg yolk are now being developed for a wide range of diseases and ailments. Ostrich antibodies against DHT and 5α-reductase have been produced and combined with antibodies against harmful skin bacteria to restore normal hair growth in AGA cases. Preliminary testing with six volunteer subjects showed that ostrich antibodies were clearly quite effective in hair regrowth. 展开更多
关键词 ALOPECIA AGA ostrich ANTIBODY
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Characterization of the Gastric Intraluminal pH and Development of the Proventricular Deep Glands during Perinatal Period in the Ostrich
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作者 Ilmars Duritis Arnis Mugurevics 《Journal of Life Sciences》 2011年第10期806-813,共8页
In this research, the state of the gastric secretion in 38 days old ostrich embryos and chicks of different age was investigated by determining the pH level in the stomach as well as the histological structure of the ... In this research, the state of the gastric secretion in 38 days old ostrich embryos and chicks of different age was investigated by determining the pH level in the stomach as well as the histological structure of the deep proventricular glands (gll. proventriculares profundi). In both parts of the stomach, on the 38th day of embryonic development pH parameters were higher than in chicks after hatching. The proventricular deep gland lobules and secretory epithelium on the 38th day of embryonic development and on the day of hatching were comparatively poorly developed. The most rapid increase of the proportion of the secretory epithelium of the proventricular deep glands was observed just before hatching and during the first week after hatching, but having reached two weeks of age it did not increase any more. The obtained results allow making a conclusion that already on the day of hatching an intensive gastric juice secretion occurs in the proventricular deep glands regardless of the stage of their histological development. The gastric intraluminal pH parameters indicate that the gastric secretion increases with chicks advancing in age, however on the day of hatching this important gastric function is biologically rather complete. 展开更多
关键词 ostrich gastric intraluminal pH deep proventricular glands.
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Population Status and Trend of the Maasai Ostrich in the Mid Kenya—Tanzania Borderland
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作者 Moses Makonjio Okello John Warui Kiringe +12 位作者 Lekishon Kenana Fiesta Warinwa Hanori Maliti Noah Wasilwa Sitati Erastus Kanga Samwel Bakari Stephen Ndambuki Philip Muruthi Nathan Gichohi Edeus Massawe David Kimutai Machoke Mwita Daniel Muteti 《Natural Resources》 2016年第10期558-579,共22页
The Maasai ostrich (Struthio camelus) is a the largest avian species in East Africa and though it’s not considered to be a species of conservation concern, some populations are on the decline and this is attributed t... The Maasai ostrich (Struthio camelus) is a the largest avian species in East Africa and though it’s not considered to be a species of conservation concern, some populations are on the decline and this is attributed to bush meat activities, predation on their eggs illegal consumption by humans, habitat destruction and forage competition with other large wildlife species. Climate change is also emerging to be another major threat due to interference with food availability which in turn interferes with the breeding rhythm. Thus, this study examined the population status, trend and distribution of the Maasai ostrich in the Southern Kenya and Northern Tanzania borderland after the 2007 to 2009 drought. The results showed that the species was found across the entire borderland but the Amboseli region had the highest number and density of Maasai ostrich (726.00 ± 100.9;0.08 ± 0.01 ostriches per km<sup>2</sup>), followed by Lake Natron area (330.8 ± 69.8;0.05 ± 0.01 ostriches per km<sup>2</sup>) and the least was in West Kilimanjaro (85.5 ± 18.0;0.03 ± 0.01 ostriches per km<sup>2</sup>). Drought caused a decline in the population of the Maasai ostrich but the Amboseli area experienced the highest decline in density (?13.44 ± 12.61) compared to other borderland sectors. However, the populations increased in most sectors after the drought, and wet season numbers and densities were higher than the dry season. The highest positive increase in number and density was in Lake Natron area (+85.65 ± 91.06) followed by West Kilimanjaro (+68.39 ± 59.54), and the least was in the Magadi area (+22.26 ± 32.05). There is a need to enhance conservation of avian species like the Maasai ostrich other than just focusing on the charismatic species such as the African elephant and black rhino. We therefore recommend joint collaboration in monitoring all large wildlife populations across the Kenya-Tanzania borderland with a view of understanding their status, trend and best management actions that can enhance their conservation. 展开更多
关键词 BORDERLAND Kenya Maasai ostrich Population Trend and Status Tanzania
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Archaeological study of ostrich eggshell beads collected from SDG site 被引量:7
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作者 WANG ChunXue1,2,3, ZHANG Yue1,3,4, GAO Xing1,3, ZHANG XiaoLing1,2,3 & WANG HuiMin1,5 1 Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044, China 2 Graduate School, Chinese Academy of Sciences, Beijing 100039, China +2 位作者 3 Joint Laboratory of Human Evolution and Archaeometry, Chinese Academy of Sciences, Beijing 100044, China 4 State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing 210008, China 5 Ningxia Provincial Institute of Archaeology, Yinchuan 750001, China 《Chinese Science Bulletin》 SCIE EI CAS 2009年第21期3887-3895,共9页
Ostrich eggshell beads and fragments collected from SDG site reflect primordial art and a kind of symbolic behavior of modern humans. Based on stratigraphic data and OSL dating, these ostrich eggshell beads are probab... Ostrich eggshell beads and fragments collected from SDG site reflect primordial art and a kind of symbolic behavior of modern humans. Based on stratigraphic data and OSL dating, these ostrich eggshell beads are probably in Early Holocene (< 10 ka BP). Two different prehistoric manufacturing pathways are usually used in the manufacture of ostrich eggshell beads in Upper Paleolithic. According to statistic analysis of the characteristics of ostrich eggshell beads, Pathway 1 is identified from these collections. In pathway 1, blanks are drilled prior to being trimmed to rough discs. They exhibit great potential for the study of the origin of primordial art and the development of ancient cultures and provide important data for studying behavioral options adopted by hominids in SDG area. In addition, they bear important implications for the origin of modern humans in East Asia. 展开更多
关键词 蛋壳 鸵鸟 收集 网站 考古 现代人类 原始艺术 旧石器时代
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基于RC缩放因子的IC物理设计时序一致性方法研究
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作者 李锦明 周焱江 《微电子学与计算机》 2025年第12期153-160,共8页
在IC物理设计中,准确地对电路中的RC参数进行合理的提取计算,是对后面可以更快更高效完成时序收敛以及设计前后时序一致性的重要保障。针对数字IC物理设计中,RC寄生参数提取的数值因工具不同而产生较大偏差,进而引发设计前后时序一致性... 在IC物理设计中,准确地对电路中的RC参数进行合理的提取计算,是对后面可以更快更高效完成时序收敛以及设计前后时序一致性的重要保障。针对数字IC物理设计中,RC寄生参数提取的数值因工具不同而产生较大偏差,进而引发设计前后时序一致性差的问题,提出了一种采用辅助工具Ostrich来对不同来源的SPEF文件进行对比,从而生成缩放因子对RC参数进行校准,并进一步修正设计。最后,项目验证WNS和TNS与签核标准的契合度均有所提升,证明了该方法能够有效提高系统的可靠性并且实现设计前后时序一致性的目的。 展开更多
关键词 物理设计 时序一致性 RC参数 ostrich 缩放因子
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不同日龄非洲鸵鸟盲肠微生物多样性分析
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作者 蒋金凤 谢洋 +6 位作者 张妍 周峰 兰忠岐 沈爽 罗青平 王家乡 李鹏 《家畜生态学报》 北大核心 2025年第3期15-21,共7页
盲肠是机体消化粗纤维,促进营养物质发酵的主要部位,有着庞大的微生物群落,许多动物的盲肠微生物已被分析研究,而非洲鸵鸟的相关研究还未见报道。因此,本试验采用宏基因组学测序技术对7日龄、30日龄、60日龄和180日龄非洲鸵鸟盲肠中的... 盲肠是机体消化粗纤维,促进营养物质发酵的主要部位,有着庞大的微生物群落,许多动物的盲肠微生物已被分析研究,而非洲鸵鸟的相关研究还未见报道。因此,本试验采用宏基因组学测序技术对7日龄、30日龄、60日龄和180日龄非洲鸵鸟盲肠中的微生物进行多样性分析。结果显示,非洲鸵鸟盲肠中的微生物群分布于9个菌门,16个菌纲,29个菌目,58个菌科和128个菌属,且随着日龄增加,盲肠中微生物的丰富度和多样性先增高后降低,其中60日龄时微生物最丰富,30日龄时微生物多样性最高。门水平上,7日龄、60日龄和180日龄均以厚壁菌门(Firmicutes)和变形菌门(Proteobacteria)为最优势菌门,而30日龄时的优势菌门为厚壁菌门、柔膜菌门(Tenericutes)和拟杆菌门(Bacteroidetes);属水平上,各日龄盲肠中的优势菌属均不相同,7日龄时不动杆菌属(Acinetobacter)、大肠埃希氏菌-志贺菌属(Escherichia-Shigella)、阿克曼菌属(Ackermansia)丰度最高,30日龄时厌氧原体属(Anaeroplasma)和拟杆菌属(Bacteroides)丰度最高,不动杆菌属和假单胞菌属(Pseudomonas)分别为60日龄和180日龄时的优势菌属;各日龄盲肠中均存在差异菌群,7日龄、30日龄、60日龄和180日龄时分别有28个、24个、8个和6个差异菌群,随着日龄增大,差异菌群的数量逐渐减少。综上显示,非洲鸵鸟盲肠微生物复杂多样,且随着日龄的增加,微生物组成及优势物种会产生变化,这为进一步研究年龄对肠道微生物的影响以及肠道微生物与机体之间的相互作用提供理论依据。 展开更多
关键词 非洲鸵鸟 盲肠 肠道微生物 宏基因组学
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Ghrelin对非洲雏鸵鸟胸腺生长发育的影响
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作者 蒋金凤 李白涛 +2 位作者 周峰 王家乡 李鹏 《中国家禽》 北大核心 2025年第1期89-96,共8页
为探明注射外源性生长素释放肽(Ghrelin)对非洲雏鸵鸟胸腺生长发育的影响,试验将16只7日龄非洲雏鸵鸟随机分为4组,每组4个重复,饲养33 d后采用趾静脉内注射不同剂量(0、10μg/kg、50μg/kg、100μg/kg)Ghrelin,取胸腺组织进行石蜡包埋... 为探明注射外源性生长素释放肽(Ghrelin)对非洲雏鸵鸟胸腺生长发育的影响,试验将16只7日龄非洲雏鸵鸟随机分为4组,每组4个重复,饲养33 d后采用趾静脉内注射不同剂量(0、10μg/kg、50μg/kg、100μg/kg)Ghrelin,取胸腺组织进行石蜡包埋、切片,采用HE染色、TUNEL技术、免疫组织化学染色、qPCR技术,对雏鸵鸟胸腺形态学、胸腺细胞凋亡及胸腺中Foxn1基因进行研究。结果显示:①与对照组相比,注射10μg/kg、50μg/kg、100μg/kg Ghrelin后,非洲雏鸵鸟胸腺指数和胸腺重量都会降低(P<0.05),其中100μg/kg剂量组的胸腺指数显著低于50μg/kg剂量组(P<0.05);②注射不同剂量Ghrelin后,随着剂量的增加,胸腺形态结构变化显著,其中50μg/kg剂量组胸腺皮质厚度和皮髓面积比明显降低(P<0.05),皮髓质交界模糊,皮质和髓质淋巴细胞减少,排列疏松,并且有少量淋巴细胞体积减小,胞浆减小,胞核固缩深染;③在胸腺的髓质部,皮质和皮髓交界处均有凋亡细胞,其中10μg/kg剂量组TUNEL染色阳性率极显著高于对照组(P<0.01);④Foxn1阳性细胞主要分布在髓质中,皮质部和皮髓交界处也有少量分布;各剂量组胸腺内Foxn1阳性细胞MD值均低于对照组,以100μg/kg剂量组显著降低(P<0.05);⑤qPCR结果显示,注射不同剂量Ghrelin的胸腺内Foxn1 mRNA表达水平均低于对照组(P<0.01),这与免疫组化得到的结果相似。综合以上结果,注射不同剂量Ghrelin会抑制雏鸵鸟胸腺的生长,诱导胸腺细胞凋亡,抑制胸腺内Foxn1基因表达,从而抑制胸腺的正常发育,影响雏鸵鸟的免疫调节能力。 展开更多
关键词 非洲鸵鸟 GHRELIN 胸腺 Foxn1基因 细胞凋亡
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南疆地区鸵鸟源枯草芽孢杆菌的分离鉴定及生物学特性分析
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作者 王辉翔 龙宝琴 +5 位作者 崔雅琪 陈贝贝 陈小雪 衣力亚尔·阿布拉江 赵健钢 焦海宏 《饲料研究》 北大核心 2025年第6期83-88,共6页
试验旨在从南疆地区非洲鸵鸟的新鲜粪便中分离鉴定枯草芽孢杆菌并研究其生物学特性。试验采用革兰氏染色和16S rRNA基因序列分析方法对分离菌株进行种属鉴定,对这些菌株的溶血性质、抗菌活性、生物学特性、生物化学特性以及药物抗性进... 试验旨在从南疆地区非洲鸵鸟的新鲜粪便中分离鉴定枯草芽孢杆菌并研究其生物学特性。试验采用革兰氏染色和16S rRNA基因序列分析方法对分离菌株进行种属鉴定,对这些菌株的溶血性质、抗菌活性、生物学特性、生物化学特性以及药物抗性进行全面分析。结果显示,试验分离鉴定出2株枯草芽孢杆菌,分别被命名为T4和T9。2株菌株在LB固体培养基上生长成为乳白色菌落,菌落特征为凸起、表面湿润、光滑且透明。T4菌株对大肠杆菌和金黄色葡萄球菌均具有抑制作用,其抑制圈直径分别为10 mm和14 mm;而T9菌株则仅对大肠杆菌表现出抑制效果,抑制圈直径为13 mm。T4和T9对多种抗生素如环丙沙星、头孢哌酮、阿奇霉素等表现出敏感性,而对苯唑西林等表现出耐药性。T4菌株的最优生长条件为接种量4%、振荡转速180 r/min、温度42℃、pH值7.0;T9菌株的最优生长条件为接种量5%、振荡转速180 r/min、温度47℃、pH值7.0。2株菌株在胆盐浓度为5%时的存活率均在30%以上,表明其具有良好的胆盐耐受能力。研究表明,从南疆地区非洲鸵鸟粪便中成功分离出2株枯草芽孢杆菌,其可作为微生物饲料添加剂候选株。 展开更多
关键词 枯草芽孢杆菌 鸵鸟 生物学特性 耐药性 抑菌能力
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非洲鸵鸟食管组织学观察 被引量:13
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作者 肖传斌 刘忠虎 +3 位作者 梁宏德 党静 高春生 王平利 《河南农业大学学报》 CAS CSCD 北大核心 2005年第1期102-105,共4页
应用常规石蜡切片,H-E染色,对非洲鸵鸟食管组织结构进行观察并与家禽和哺乳动物的食管进行比较.结果表明:非洲鸵鸟食管黏膜上皮为复层扁平上皮,但角质化不明显,固有膜内食管腺丰富,由腺细胞围成的管泡状腺直接开口于黏膜上皮,分泌大量黏... 应用常规石蜡切片,H-E染色,对非洲鸵鸟食管组织结构进行观察并与家禽和哺乳动物的食管进行比较.结果表明:非洲鸵鸟食管黏膜上皮为复层扁平上皮,但角质化不明显,固有膜内食管腺丰富,由腺细胞围成的管泡状腺直接开口于黏膜上皮,分泌大量黏液.食管肌层非常发达,分为内环肌、中纵肌、外环肌3层,环肌很厚,与纵肌的比例约为3:1.外膜是一层薄的纤维膜,内含丰富的血管,淋巴组织和神经. 展开更多
关键词 食管黏膜上皮 组织学观察 常规石蜡切片 血管 淋巴组织 纤维膜 神经 非洲鸵鸟 家禽 黏液
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鸵鸟源隐孢子虫的种类鉴定及其生物学特性研究 被引量:15
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作者 孙铭飞 张龙现 +3 位作者 宁长申 梁宏德 菅复春 张敏 《中国农业科学》 CAS CSCD 北大核心 2007年第7期1528-1534,共7页
【目的】掌握鸵鸟隐孢子虫病的流行范围和流行动态,以及研究鸵鸟源隐孢子虫的生物学特性。【方法】应用饱和蔗糖溶液漂浮法检查鸵鸟新鲜粪样404份,详细观察、测量卵囊形态大小;收集纯化阳性样品中的隐孢子虫卵囊,分别人工经口感染幼鸵... 【目的】掌握鸵鸟隐孢子虫病的流行范围和流行动态,以及研究鸵鸟源隐孢子虫的生物学特性。【方法】应用饱和蔗糖溶液漂浮法检查鸵鸟新鲜粪样404份,详细观察、测量卵囊形态大小;收集纯化阳性样品中的隐孢子虫卵囊,分别人工经口感染幼鸵鸟、雏鸡和小白鼠并利用组织病理学试验技术研究其生物学特性。【结果】检测到阳性样品10份,总阳性率为2.48%(10/404),且感染率与鸵鸟日龄有一定的关系。其卵囊平均大小为5.43×4.38μm(4.0~6.0×3.7~5.0μm),形状指数1.24(长/宽),(n=50)。分离到的卵囊能成功感染雏鸡和幼鸵鸟,但不能感染小白鼠。受试雏鸡具有明显的呼吸道症状,而幼龄鸵鸟呼吸道症状较轻微。此外,在两种动物体内的排卵囊规律差别较大,在鸵鸟体内出现多个排卵囊高峰。虫体主要寄生于雏鸡和幼鸵鸟的泄殖腔和法氏囊,引起法氏囊萎缩、微绒毛大量脱落等病理变化。【结论】根据其卵囊形态、宿主范围和虫体寄生部位等生物学特性,结合文献报道,笔者在国内发现并初步鉴定出鸵鸟源隐孢子虫为贝氏隐孢子虫(Cryptosporidium baileyi)。 展开更多
关键词 鸵鸟 隐孢子虫 交叉感染 电镜 种类鉴定
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非洲雏鸵鸟十二指肠发育的形态学研究 被引量:21
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作者 王家乡 彭克美 +6 位作者 杜安娜 唐丽 位兰 王岩 李升和 宋卉 靳二辉 《中国兽医学报》 CAS CSCD 北大核心 2008年第12期1419-1422,共4页
采用大体解剖学、组织化学和形态计量学的方法研究了0~3月龄非洲鸵鸟十二指肠的发育过程。观察了十二指肠的相对质量(相对于体质量),绒毛的高度、宽度、肌层厚度、肠腺隐窝的深度、肠腺的密度、绒毛杯状细胞、肠腺杯状细胞的数量变... 采用大体解剖学、组织化学和形态计量学的方法研究了0~3月龄非洲鸵鸟十二指肠的发育过程。观察了十二指肠的相对质量(相对于体质量),绒毛的高度、宽度、肌层厚度、肠腺隐窝的深度、肠腺的密度、绒毛杯状细胞、肠腺杯状细胞的数量变化。观察到十二指肠的相对质量在90日龄达到高峰;肠绒毛的高度、肌层厚度、肠腺隐窝的深度、肠腺的密度与日龄呈正相关;肠绒毛和肠腺的宽度在90日龄时达到峰值;绒毛杯状细胞、肠腺杯状细胞的数量从初生至45日龄随日龄增加而增加,45日龄至90日龄随日龄增加而减少,在45日龄达顶峰。结果表明,0~3月龄十二指肠的发育不完善,必须重视此阶段的饲养管理。 展开更多
关键词 非洲雏鸵鸟 十二指肠 形态学 发育
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沙地刚性轮构型仿生设计及牵引性能数值分析 被引量:16
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作者 张锐 罗刚 +5 位作者 薛书亮 杨明明 刘芳 张四华 潘润铎 李建桥 《农业工程学报》 EI CAS CSCD 北大核心 2015年第3期122-128,共7页
为了提高车轮牵引性能,改善车辆在松散沙土介质环境的通过能力,该文以善于沙地奔跑的鸵鸟足部关键部位—足趾甲为仿生原型,通过仿生优化轮刺结构,设计出具有高牵引性能的仿生轮刺式沙地刚性轮,并以一种模拟月壤作为试验松散沙土介质材料... 为了提高车轮牵引性能,改善车辆在松散沙土介质环境的通过能力,该文以善于沙地奔跑的鸵鸟足部关键部位—足趾甲为仿生原型,通过仿生优化轮刺结构,设计出具有高牵引性能的仿生轮刺式沙地刚性轮,并以一种模拟月壤作为试验松散沙土介质材料,采用离散元软件PFC2D?的内置语言FISH和相关命令,建立了适用于非规则结构刚性轮的轮壤相互作用动态模拟系统,并获得试验验证。通过仿生轮刺式刚性轮与模拟月壤相互作用离散元模拟,并与矩形轮刺式刚性轮模拟结果对照,从轮下模拟月壤颗粒细观运动、接触力场、速度场以及车轮挂钩牵引力角度,验证了仿生轮刺式刚性轮具有优越的牵引性能,在车轮滑转率50%的稳定运行状态下,仿生轮刺式刚性轮的牵引性能可提高5.2%左右。该研究为提高刚性轮在松散沙土介质环境中的牵引性能提供了全新设计和研究手段。 展开更多
关键词 车辆 仿生学 数值分析 沙地刚性轮 鸵鸟足趾甲 牵引性能 离散元模拟
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