Broad-spectrum resistance(BSR)reduces pathogen-related yield losses in crops such as rice(Oryza sativa).To achieve BSR,traditional breeding has focused on the time-intensive process of incorporating resistance(R)genes...Broad-spectrum resistance(BSR)reduces pathogen-related yield losses in crops such as rice(Oryza sativa).To achieve BSR,traditional breeding has focused on the time-intensive process of incorporating resistance(R)genes into elite germplasm.Now,CRISPR/Cas9-mediated genome editing makes it possible to modify susceptibility(S)genes to rapidly achieve BSR in rice.However,identifying S genes remains challenging.Here,we analyzed transcriptome data and determined that OsJAC1,encoding a mannosebinding jacalin-related lectin,is significantly induced upon infection by the rice blast fungus Magnaporthe oryzae.To explore the role of OsJAC1 in BSR in rice,we generated OsJAC1 overexpression and knockout mutant lines in the rice ZH11(Zhonghua 11)background and performed pathogen inoculation assays,revealing that OsJAC1 negatively regulates resistance against M.oryzae and the bacterial blight pathogen Xanthomonas oryzae pv.oryzae.Further evidence of defense responses,such as a reactive oxygen species burst,defense-related gene expression,and MAPK phosphorylation,also supports the role of OsJAC1 as a negative regulator of plant immunity.To further validate the function of OsJAC1,we knocked out OsJAC1 in the Nipponbare(NPB)background.The resulting NPB-osjac1^(cas9)plants showed enhanced defense responses and resistance against M.oryzae.Notably,the osjac1 mutants did not compromise agronomic traits in the ZH11 or NPB background.Hence,OsJAC1 could be regarded as an S gene and could serve as a potential target in rice breeding programs,providing valuable insights for crop improvement.展开更多
Lectin plays an Important role In defense signaling In plants, but its function In plant growth and development is not well known. Previously, we cloneds rice （Oryza sativa L.） gene OsJAC1 encoding s msnnose-blndlng...Lectin plays an Important role In defense signaling In plants, but its function In plant growth and development is not well known. Previously, we cloneds rice （Oryza sativa L.） gene OsJAC1 encoding s msnnose-blndlng Jscslln-relsted lectln, and found that OsJAC1 was Jssmonlc acid （JA） Inducible. Here we cloned the promoter of OsJAC1, and GUS activity was detected In young roots, coleoptlles, sheaths, leaves, nodes of stems, stems, rschlses, pistils, stsmens and lemmss of OsJAC1：：GUS trsnsgenlc rice, suggesting that OsJAC1 Is s constitutive expression gene In rice. Moreover, OsJAC1-overexpressed （Ubi：：OsJAC1） rice showed dwarfism with shorter coleptlles resulting from the failure of cell elongation of coleoptlles. In addition, compared with coleoptlles of wild-type plants, those of OsJAC1 overexpresslon rice were more sensitive to JA treatment. These data revealed that, besides Its roles in defense response, lectin plays an Important role in rice growth and development.展开更多
基金supported by the Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSCB-202301)the National Natural Science Foundation of China(32272505 and 32161143009)to Yuese Ning+1 种基金the National Natural Science Foundation of China(32372141)the China Postdoctoral Science Foundation(2023M733827)to Hui Tao.
文摘Broad-spectrum resistance(BSR)reduces pathogen-related yield losses in crops such as rice(Oryza sativa).To achieve BSR,traditional breeding has focused on the time-intensive process of incorporating resistance(R)genes into elite germplasm.Now,CRISPR/Cas9-mediated genome editing makes it possible to modify susceptibility(S)genes to rapidly achieve BSR in rice.However,identifying S genes remains challenging.Here,we analyzed transcriptome data and determined that OsJAC1,encoding a mannosebinding jacalin-related lectin,is significantly induced upon infection by the rice blast fungus Magnaporthe oryzae.To explore the role of OsJAC1 in BSR in rice,we generated OsJAC1 overexpression and knockout mutant lines in the rice ZH11(Zhonghua 11)background and performed pathogen inoculation assays,revealing that OsJAC1 negatively regulates resistance against M.oryzae and the bacterial blight pathogen Xanthomonas oryzae pv.oryzae.Further evidence of defense responses,such as a reactive oxygen species burst,defense-related gene expression,and MAPK phosphorylation,also supports the role of OsJAC1 as a negative regulator of plant immunity.To further validate the function of OsJAC1,we knocked out OsJAC1 in the Nipponbare(NPB)background.The resulting NPB-osjac1^(cas9)plants showed enhanced defense responses and resistance against M.oryzae.Notably,the osjac1 mutants did not compromise agronomic traits in the ZH11 or NPB background.Hence,OsJAC1 could be regarded as an S gene and could serve as a potential target in rice breeding programs,providing valuable insights for crop improvement.
基金Supported by the Innovation Grant of the Chinese Academy of Sciences, and the National Natural Science Foundation of China （30470167）.
文摘Lectin plays an Important role In defense signaling In plants, but its function In plant growth and development is not well known. Previously, we cloneds rice （Oryza sativa L.） gene OsJAC1 encoding s msnnose-blndlng Jscslln-relsted lectln, and found that OsJAC1 was Jssmonlc acid （JA） Inducible. Here we cloned the promoter of OsJAC1, and GUS activity was detected In young roots, coleoptlles, sheaths, leaves, nodes of stems, stems, rschlses, pistils, stsmens and lemmss of OsJAC1：：GUS trsnsgenlc rice, suggesting that OsJAC1 Is s constitutive expression gene In rice. Moreover, OsJAC1-overexpressed （Ubi：：OsJAC1） rice showed dwarfism with shorter coleptlles resulting from the failure of cell elongation of coleoptlles. In addition, compared with coleoptlles of wild-type plants, those of OsJAC1 overexpresslon rice were more sensitive to JA treatment. These data revealed that, besides Its roles in defense response, lectin plays an Important role in rice growth and development.
