目的O-GlcNAc糖基化(O-linked beta-N-acetylglucosamine,O-GlcNAc)和Yes相关蛋白1(yes-associated protein 1,YAP1)是否会发生糖基化、在结直肠癌中的表达情况及发挥作用的机制都尚未得到充分阐明。本研究旨在借助癌症基因组图谱(the c...目的O-GlcNAc糖基化(O-linked beta-N-acetylglucosamine,O-GlcNAc)和Yes相关蛋白1(yes-associated protein 1,YAP1)是否会发生糖基化、在结直肠癌中的表达情况及发挥作用的机制都尚未得到充分阐明。本研究旨在借助癌症基因组图谱(the cancer genome atlas,TCGA)数据库、组织标本和细胞系探究O-GlcNAc糖基化和YAP1糖基化在结直肠中所发挥的作用。方法采用免疫组化法检测O-GlcNAc和OGT蛋白(O-linked-N-acetylglucosamine transferase,OGT)在结直肠组织和癌旁组织中的表达情况;运用qPCR检测结直肠组织和癌旁组织中OGT mRNA表达水平;借助在线分析TCGA数据库,了解OGT的表达情况及其对预后产生的影响;通过Western Blot检测OSMI-1对O-GlcNAc糖基化的抑制作用;采用CCK-8和Transwell检测,在抑制O-GlcNAc糖基化后,观察肠癌细胞增殖、侵袭及迁移能力的变化;借助数据库分析YAP1的表达情况及其O-GlcNAc糖基化位点,并经质谱分析确定YAP1是否发生O-GlcNAc糖基化修饰;运用Co-IP和免疫荧光实验验证YAP1和OGT之间的结合关系;采用qPCR和免疫荧光明确抑制O-GlcNAc糖基化后YAP1表达位置的改变以及下游效应基因表达的改变情况。结果研究发现,OGT蛋白和mRNA在结直肠组织中的表达水平明显升高,但对患者的预后并无明显影响;OSMI-1能够抑制肠癌细胞的O-GlcNAc糖基化,并且抑制细胞增殖、侵袭与迁移能力;YAP1可与OGT结合并发生O-GlcNAc糖基化,当抑制O-GlcNAc糖基化后,YAP1入核数量减少,转录活性降低。结论在结直肠癌中,O-GlcNAc糖基化水平呈现升高趋势,OGT和YAP1结合,使YAP1发生O-GlcNAc糖基化修饰,促进其转录活性。展开更多
Lignin is the only largest renewable aromatic resource in nature.Currently,most lignin is underutilized for low-value applications due to the complex structure and recalcitrant chemical properties.Over the past decade...Lignin is the only largest renewable aromatic resource in nature.Currently,most lignin is underutilized for low-value applications due to the complex structure and recalcitrant chemical properties.Over the past decades,extensive research has been devoted to valorizing lignin into aromatic N-heterocycles in the presence of nitrogen sources.It overcomes the element limitation,expands the products portfolio and would play a momentous role in value-added biorefinery concept.In this review,the latest research progress in the synthesis of N-heterocyclic compounds from lignin,lignin model compounds,and lignin-derived monomers(phenols,aromatic alcohols,aldehydes,ketones,and ethers)is presented.According to the structural characteristics of the products,these achievements are classified by the construction of five-,six-,and seven-membered N-heterocyclic compounds through one-step,multi-step,or one-pot multi-step reactions.Furthermore,the tailor-designed routes and catalytic systems,along with the reaction mechanisms/pathways involved are entirely discussed to elucidate the challenges regarding the structural complexity of lignin,the incompatible catalysis for C–O cleavage and C–N formation,as well as the nitrogen-heterocyclic ring construction.The prospects,future research efforts and process developments for the refining of lignin into aromatic N-heterocyclic compounds are outlined in terms of economy,environmental friendliness,and safety so as to draw some guidelines for lignin valorization.展开更多
建立了一种在温和条件下,通过可见光催化合成一系列N-芳基吲哚类化合物的方法。该方法在室温条件下,以邻烯基苯胺和重氨萘酮为模板底物,以曙红Y作为光催化剂,在40 W 460 nm的蓝色LED灯照射下,合成一系列N-芳基吲哚衍生物,最高产率可达到...建立了一种在温和条件下,通过可见光催化合成一系列N-芳基吲哚类化合物的方法。该方法在室温条件下,以邻烯基苯胺和重氨萘酮为模板底物,以曙红Y作为光催化剂,在40 W 460 nm的蓝色LED灯照射下,合成一系列N-芳基吲哚衍生物,最高产率可达到74%。该合成路径具有底物适用范围广、经济实用等特点,为N-芳基吲哚衍生物合成提供了一种经济简便的方法。展开更多
文摘目的O-GlcNAc糖基化(O-linked beta-N-acetylglucosamine,O-GlcNAc)和Yes相关蛋白1(yes-associated protein 1,YAP1)是否会发生糖基化、在结直肠癌中的表达情况及发挥作用的机制都尚未得到充分阐明。本研究旨在借助癌症基因组图谱(the cancer genome atlas,TCGA)数据库、组织标本和细胞系探究O-GlcNAc糖基化和YAP1糖基化在结直肠中所发挥的作用。方法采用免疫组化法检测O-GlcNAc和OGT蛋白(O-linked-N-acetylglucosamine transferase,OGT)在结直肠组织和癌旁组织中的表达情况;运用qPCR检测结直肠组织和癌旁组织中OGT mRNA表达水平;借助在线分析TCGA数据库,了解OGT的表达情况及其对预后产生的影响;通过Western Blot检测OSMI-1对O-GlcNAc糖基化的抑制作用;采用CCK-8和Transwell检测,在抑制O-GlcNAc糖基化后,观察肠癌细胞增殖、侵袭及迁移能力的变化;借助数据库分析YAP1的表达情况及其O-GlcNAc糖基化位点,并经质谱分析确定YAP1是否发生O-GlcNAc糖基化修饰;运用Co-IP和免疫荧光实验验证YAP1和OGT之间的结合关系;采用qPCR和免疫荧光明确抑制O-GlcNAc糖基化后YAP1表达位置的改变以及下游效应基因表达的改变情况。结果研究发现,OGT蛋白和mRNA在结直肠组织中的表达水平明显升高,但对患者的预后并无明显影响;OSMI-1能够抑制肠癌细胞的O-GlcNAc糖基化,并且抑制细胞增殖、侵袭与迁移能力;YAP1可与OGT结合并发生O-GlcNAc糖基化,当抑制O-GlcNAc糖基化后,YAP1入核数量减少,转录活性降低。结论在结直肠癌中,O-GlcNAc糖基化水平呈现升高趋势,OGT和YAP1结合,使YAP1发生O-GlcNAc糖基化修饰,促进其转录活性。
文摘Lignin is the only largest renewable aromatic resource in nature.Currently,most lignin is underutilized for low-value applications due to the complex structure and recalcitrant chemical properties.Over the past decades,extensive research has been devoted to valorizing lignin into aromatic N-heterocycles in the presence of nitrogen sources.It overcomes the element limitation,expands the products portfolio and would play a momentous role in value-added biorefinery concept.In this review,the latest research progress in the synthesis of N-heterocyclic compounds from lignin,lignin model compounds,and lignin-derived monomers(phenols,aromatic alcohols,aldehydes,ketones,and ethers)is presented.According to the structural characteristics of the products,these achievements are classified by the construction of five-,six-,and seven-membered N-heterocyclic compounds through one-step,multi-step,or one-pot multi-step reactions.Furthermore,the tailor-designed routes and catalytic systems,along with the reaction mechanisms/pathways involved are entirely discussed to elucidate the challenges regarding the structural complexity of lignin,the incompatible catalysis for C–O cleavage and C–N formation,as well as the nitrogen-heterocyclic ring construction.The prospects,future research efforts and process developments for the refining of lignin into aromatic N-heterocyclic compounds are outlined in terms of economy,environmental friendliness,and safety so as to draw some guidelines for lignin valorization.
文摘建立了一种在温和条件下,通过可见光催化合成一系列N-芳基吲哚类化合物的方法。该方法在室温条件下,以邻烯基苯胺和重氨萘酮为模板底物,以曙红Y作为光催化剂,在40 W 460 nm的蓝色LED灯照射下,合成一系列N-芳基吲哚衍生物,最高产率可达到74%。该合成路径具有底物适用范围广、经济实用等特点,为N-芳基吲哚衍生物合成提供了一种经济简便的方法。
