With the development of urban infrastructure,it is inevitable that shield tunnels will undercross intercity railways.However,the safe operation of intercity railways requires strict subgrade deformation.On the basis o...With the development of urban infrastructure,it is inevitable that shield tunnels will undercross intercity railways.However,the safe operation of intercity railways requires strict subgrade deformation.On the basis of the engineering background of the Lianghu Tunnel in Wuhan,the three-dimensional centrifuge test and numerical back analysis were used to study the development of subgrade surface settlement during shield tunneling.A three-dimensional numerical model with the same size as the prototype was subsequently established to further study the settlement development and torsion behavior of the subgrade during tunnel excavation.The results show that the maximum settlement point of the transverse settlement trough gradually moves to the tunnel axis during tunnel excavation and that the entire subgrade experiences torsional deformation.Moreover,the effect of the intersection angle between the axes of the tunnel and the subgrade on the surface settlement of the subgrade was further studied.The results show that the intersection angle has no effect on the maximum settlement,but the width of the settlement trough increases gradually with increasing angle.Finally,on the basis of the soil arching effect caused by tunnel excavation,the subgrade settlement during tunnel excavation is reduced by reinforcing the soil in different zones of soil arching.The results show that the settlement of the subgrade caused by the shield tunnel can be effectively controlled by adding reinforcement directly to the top of the tunnel,and the maximum settlement of the subgrade surface is reduced from 24.41 mm to 9.47 mm,a reduction of approximately 61.2%.展开更多
A full-scale research study was conducted during the bored tunnelling of the Klang Valley Mass Rapid Transit-Putrajaya Line beneath an existing building structure in Kuala Lumpur,Malaysia.The primary objective was to ...A full-scale research study was conducted during the bored tunnelling of the Klang Valley Mass Rapid Transit-Putrajaya Line beneath an existing building structure in Kuala Lumpur,Malaysia.The primary objective was to investigate the tunnel-soil-pile interaction at various stages of tunnel excavation.This study combined field measurements and three-dimensional(3D)numerical analysis to understand the transient effects of TBM tunnelling on a loaded pile.An experimental pile was instrumented with vibrating wire strain gauges,an inclinometer,and distributed fibre optic sensors using Brillouin optical time domain analysis.The pile was pre-loaded and continuously monitored in real-time throughout the tunnel construction process.The 3D finite element modelling was used to simulate the pile’s transient responses based on actual tunnel boring machine(TBM)driving data.The study revealed that the zone of influence due to tunnelling effects extended from y¼2D to y¼4D,with the peak effect observed at y¼1D to 1.5D,where D represents the tunnel diameter.The analysis of axial load patterns highlighted transient responses,including tensile loads below the tunnel invert,which propagated upward and subsided due to negative skin friction.The maximum downdrag load observed reached 56%e71%of the pile’s working load.Additionally,pile movement patterns indicated outward deflections as the TBM approached and a return toward the tunnel post-passage,aligning with the predicted behaviour in a negative face loss scenario.This validated numerical framework provides a solid foundation for further parametric studies and enhances the understanding of tunnel-soil-pile interactions.展开更多
Shotcrete is one of the common solutions for shallow sliding.It works by forming a protective layer with high strength and cementing the loose soil particles on the slope surface to prevent shallow sliding.However,the...Shotcrete is one of the common solutions for shallow sliding.It works by forming a protective layer with high strength and cementing the loose soil particles on the slope surface to prevent shallow sliding.However,the solidification time of conventional cement paste is long when shotcrete is used to treat cohesionless soil landslide.The idea of reinforcing slope with polyurethane solidified soil(i.e.,mixture of polyurethane and sand)was proposed.Model tests and finite element analysis were carried out to study the effectiveness of the proposed new method on the emergency treatment of cohesionless soil landslide.Surcharge loading on the crest of the slope was applied step by step until landslide was triggered so as to test and compare the stability and bearing capacity of slope models with different conditions.The simulated slope displacements were relatively close to the measured results,and the simulated slope deformation characteristics were in good agreement with the observed phenomena,which verifies the accuracy of the numerical method.Under the condition of surcharge loading on the crest of the slope,the unreinforced slope slid when the surcharge loading exceeded 30 k Pa,which presented a failure mode of local instability and collapse at the shallow layer of slope top.The reinforced slope remained stable even when the surcharge loading reached 48 k Pa.The displacement of the reinforced slope was reduced by more than 95%.Overall,this study verifies the effectiveness of polyurethane in the emergency treatment of cohesionless soil landslide and should have broad application prospects in the field of geological disasters concerning the safety of people's live.展开更多
Heat treatment processes, such as annealing and quenching, are crucial in determining residual stress evolution, microstructural changes and mechanical properties of metallic materials, with residual stresses playing ...Heat treatment processes, such as annealing and quenching, are crucial in determining residual stress evolution, microstructural changes and mechanical properties of metallic materials, with residual stresses playing a greater role in the performance of components. This paper investigates the effect of heat treatment on residual stresses induced in AISI 1025, manufactured using LENS. Finite element model was developed and simulated to analyze residual stress development. AISI 1025 samples suitable for tool and die applications in Fused Deposition Modelling (FDM) filament production, were fabricated using Laser Engineered Net Shaping (LENS) process, followed by heat treatment where annealing and quenching processes were done. The material’s microstructure, residual stress and hardness of heat-treated samples under investigation, were compared against the as-built samples. The results indicated that after annealing, tensile residual stresses were reduced by 93%, resulting in a reduced crack growth rate, compared to the as-built sample, although the hardness was reduced significantly by 25%. On the other hand, high tensile residual stresses of 425 ± 14 MPa were recorded after quenching process with an improvement of hardness by 21%.展开更多
Software systems are vulnerable to security breaches as they expand in complexity and functionality.The confidentiality,integrity,and availability of data are gravely threatened by flaws in a system’s design,implemen...Software systems are vulnerable to security breaches as they expand in complexity and functionality.The confidentiality,integrity,and availability of data are gravely threatened by flaws in a system’s design,implementation,or configuration.To guarantee the durability&robustness of the software,vulnerability identification and fixation have become crucial areas of focus for developers,cybersecurity experts and industries.This paper presents a thorough multi-phase mathematical model for efficient patch management and vulnerability detection.To uniquely model these processes,the model incorporated the notion of the learning phenomenon in describing vulnerability fixation using a logistic learning function.Furthermore,the authors have used numerical methods to approximate the solution of the proposed framework where an analytical solution is difficult to attain.The suggested systematic architecture has been demonstrated through statistical analysis using patch datasets,which offers a solid basis for the research conclusions.According to computational research,learning dynamics improves security response and results in more effective vulnerability management.The suggested model offers a systematic approach to proactive vulnerability mitigation and has important uses in risk assessment,software maintenance,and cybersecurity.This study helps create more robust software systems by increasing patch management effectiveness,which benefits developers,cybersecurity experts,and sectors looking to reduce security threats in a growing digital world.展开更多
With the increasing development of deepburied engineering projects,rockburst disasters have become a frequent concern.Studies have indicated that tunnel diameter is a critical factor influencing the occurrence of rock...With the increasing development of deepburied engineering projects,rockburst disasters have become a frequent concern.Studies have indicated that tunnel diameter is a critical factor influencing the occurrence of rockbursts.To investigate the influence of tunnel diameter on the deformation and failure characteristics of surrounding rock,large-sized rocklike gypsum specimens were tested using a selfdeveloped true triaxial rockburst loading system containing circular tunnels with three different diameters(D=0.07 m,0.11 m,and 0.15 m).Acoustic emission monitoring,together with a miniature intelligent camera,was employed to analyze the entire process,focusing on macroscopic failure patterns,fragment characteristics,and underlying failure mechanisms.In addition,theoretical analyses were carried out and combined with numerical simulations to investigate the differences in energy evolution associated with rockburst physical models.The results indicate that:(1)The rockburst process with different tunnel diameters consistently evolved through three distinct stages—initial particle ejection,crack propagation accompanied by flake spalling,and,finally,fragment ejection leading to the formation of a‘V'-shaped notch.(2)Increasing tunnel diameter reduces rockburst failure load while increasing surrounding rock damage extent,total mass and average size of ejected fragments.Additionally,shear failure proportion decreases with tensile failure becoming increasingly dominant.(3)Larger tunnel diameters reduce the attenuation rate of elastic strain energy,thereby expanding the zone of elastic strain energy accumulation and disturbance and creating conditions for larger volume rockburst.(4)Larger tunnel diameters result in a smaller principal stress ratio at equivalent distances in the surrounding rock,indicating a higher likelihood of tensile failure.(5)Numerical analyses further reveal that larger tunnel diameters reduce the maximum elastic strain energy density around the tunnel,lowering the energy released per unit volume of rockburst fragments and their ejection velocities.However,both the total failure volume and overall energy release from rockburst increase.Model experiments with different tunnel diameters are of great significance for optimizing engineering design and parameter selection,as well as guiding tunnel construction under complex geological conditions.展开更多
The internal and external flow fields during vented explosions of methane were characterized through numerical simulation,and the capability of numerical simulation thereof was validated by previous experimental data ...The internal and external flow fields during vented explosions of methane were characterized through numerical simulation,and the capability of numerical simulation thereof was validated by previous experimental data at three ignition positions.The venting mechanism was revealed by the simulated concentration distribution,temperature profile,and airflow velocity.The results show rear ignition results in the external methane mass distribution taking the form of"mushroom"and columnar flames in the external space,which can be expressed as a third-order polynomial relationship with distance;central ignition forms a relationship of the form y=AxB.Front ignition causes the temperature to show a tendency to repeated oscillations(rising,falling,and rising).Central ignition generates the maximum vented airflow velocity(V_(max)=320 m/s)upon vent opening.The results indicate that it is acceptable to apply numerical simulation of methane explosions in practice.展开更多
This study employs the Smoothed Particle Hydrodynamics(SPH)method to develop a computational fluid dynamics(CFD)model for analyzing the interaction between rogue waves and mooring systems.Four floating body configurat...This study employs the Smoothed Particle Hydrodynamics(SPH)method to develop a computational fluid dynamics(CFD)model for analyzing the interaction between rogue waves and mooring systems.Four floating body configurations are investigated:(1)dual rectangular prisms,(2)rectangular prism–sphere composites,(3)sphere–rectangular prism composites,and(4)dual spheres.These configurations are systematically evaluated under varying mooring conditions to assess their hydrodynamic performance and wave attenuation capabilities.The model accurately captures the complex fluid–structure interaction dynamics between moored floating breakwaters and incident wave fields.Among the configurations,the dual rectangular prism system demonstrates superior performance in both wave dissipation and mooring force reduction.Under conditions involving dual wave makers,the influence of floating body shape and number on wave height is found to be minimal.However,dual-body arrangements consistently outperform single-body setups in terms of both energy dissipation and structural stability.From a cost-efficiency perspective,the configuration comprising two rectangular prisms connected via a single mooring system offers significant advantages in material usage and deployment feasibility.展开更多
The pressure wave generated by an urban-rail vehicle when passing through a tunnel affects the comfort of passengersand may even cause damage to the train and related tunnel structures.Therefore,controlling the trains...The pressure wave generated by an urban-rail vehicle when passing through a tunnel affects the comfort of passengersand may even cause damage to the train and related tunnel structures.Therefore,controlling the trainspeed in the tunnel is extremely important.In this study,this problem is investigated numerically in the frameworkof the standard k-εtwo-equation turbulence model.In particular,an eight-car urban rail train passingthrough a tunnel at different speeds(140,160,180 and 200 km/h)is considered.The results show that the maximumaerodynamic drag of the head and tail cars is most affected by the running speed.The pressure at selectedmeasuring points on the windward side of the head car is very high,and the negative pressure at the side windowof the driver’s cab of the tail car is also very large.From the head car to the tail car,the pressure at the same heightgradually decreases.The positive pressure peak at the head car and the negative pressure peak at the tail car aregreatly affected by the speed.展开更多
Enhancing the fermentation efficiency of waste in waste warehouses is pivotal for accelerating the pyrolysis process and minimizing harmful gas emissions.This study proposes an integrated approach,combining hot air in...Enhancing the fermentation efficiency of waste in waste warehouses is pivotal for accelerating the pyrolysis process and minimizing harmful gas emissions.This study proposes an integrated approach,combining hot air injection with dual atomizing nozzles,for the thermal treatment of waste piles.Numerical simulations are employed to investigate the influence of various parameters,namely,nozzle height,nozzle tilt angle,inlet air velocity and air temperature,on the droplet diffusion process,spread area,droplet temperature,and droplet size distribution.The results show that reducing the nozzle height increases the temperature of droplets upon their deposition on the waste pile.Specifically,when the nozzle height is lowered to 1.5 m,the temperature of the droplets reaching the waste pile is 1℃higher than when the nozzle height is set at 2 m.Furthermore,an increase in the nozzle tilt angle expands the overlapping heating area.For instance,when the nozzle angle is increased from 15°to 30°,the overlapping spread area expands by 3.21 m2.Additionally,increasing the inlet air velocity enhances the droplet diffusion range.At an air velocity of 2 m/s,the droplet diffusion range grows to 14.4 m,representing a 6.7%increase compared to the nowind condition.While the average droplet diameter decreases to 1.53 mm,the droplet temperature decreases by 1℃.Moreover,the droplet temperature is found to become smaller as the ambient temperature inside the waste warehouse declines.Specifically,a 5℃reduction in the ambient temperature results in a 1℃decrease in the average temperature of the atomized droplets.The study concludes that a nozzle height of 1.5 m and a nozzle tilt angle of 30°effectively meet practical heating requirements.展开更多
Both the complex geometrical morphology of rough-walled rock fractures and the nonlinearity of fluid flow contribute to resistance in fluid flow through rock fractures.The interactions of the shear-flow process furthe...Both the complex geometrical morphology of rough-walled rock fractures and the nonlinearity of fluid flow contribute to resistance in fluid flow through rock fractures.The interactions of the shear-flow process further complicate the characterisation of flow behaviours in rock fractures.In this study,an improved friction factor model involving both the effects of viscous and inertial forces is presented based on the Forchheimer equation.The model incorporates two key variables,i.e.Reynolds number and relative roughness,which reflect the effects of flow regimes and fracture roughness,respectively.The changes in geometrical parameters induced by shearing are considered,with the peak asperity height predicted through a correlation with post-peak roughness degradation.The hydraulic aperture during shearing is estimated using a suggested equation that accounts for the mobilised contact area ratio and variable aperture distribution.The parametric sensitivity analysis reveals that shear-induced changes in fracture geometry enhance the flow nonlinearity in rock fractures.The model performs well in predicting the friction factor based on two validation criteria.Then,the proposed friction factor model is incorporated into the three-dimensional distinct element code(3DEC)in the form of the Darcy-Weisbach equation.Coupled with the numerically implemented mechanical model and hydraulic aperture prediction model,numerical simulations of coupled shear-flow processes in single rock fractures are conducted.The simulation outcomes are validated through comparison with the experimental results,showing acceptable agreement and demonstrating that the numerical model is capable of accurately evaluating the hydro-mechanical coupling behaviour during the shearing of rock fractures.展开更多
Produced water reinjection is a common strategy in offshore oilfield operations,yet the presence of solid particles in produced water can lead to localized formation pressure buildup,increasing the risk of rock fractu...Produced water reinjection is a common strategy in offshore oilfield operations,yet the presence of solid particles in produced water can lead to localized formation pressure buildup,increasing the risk of rock fracturing and leakage.In this study,we present an integrated experimental and numerical investigation to quantify the effects of particle migration on formation pressure and the spatial diffusion of injected water.Dynamic plugging experiments were performed to systematically examine the influence of injection rate and injection volume on core permeability.Results demonstrate that higher injection rates substantially reduce permeability,and the derived relationship between permeability and injection volume enables dynamic assessment of permeability evolution during reinjection.Complementary numerical simulations explored the impacts of injection length,particle concentration,and injection rate on formation pressure and diffusion behavior.Findings indicate that extending the injection section promotes pressure distribution and enlarges the diffusion area,whereas elevated particle concentrations and injection rates accelerate formation plugging,causing rapid pressure rise and constrained diffusion.展开更多
A self-centering bridge bent equipped with energy-dissipation(ED)beams is proposed.Quasi-static tests are conducted on self-centering bridge bents,both with and without ED beams,to validate the accuracy of the corresp...A self-centering bridge bent equipped with energy-dissipation(ED)beams is proposed.Quasi-static tests are conducted on self-centering bridge bents,both with and without ED beams,to validate the accuracy of the corresponding numerical models.The effects of various param-eters,such as the web area of ED beams,prestressing force of tendons,tendon arrangements,and number of column segments,on the seismic performance of self-centering bridge bents with ED beams are evaluated using the validated numerical model.The results demonstrate that the nu-merical models accurately replicate the quasi-static test results,with average errors in the lateral force remaining below 9.6%.The web area of ED beams significantly affects the strength,cumulative energy dissipation,and relative self-centering index(RSI)of the self-centering bridge bents.Increasing the prestressing force enhances the lateral force and self-centering capability of the bridge bents but has minimal effect on their ED capacity.Reducing the num-ber of segments in each column enhances the lateral force and cumulative hysteretic energy dissipation of the self-centering bridge bents while exerting an insignificant effect on the RSI.Thus,the proposed novel system is highly suitable for doubleor multicolumn piers supporting bridges in regions prone to strong earthquakes.展开更多
Energy resolution calibration is crucial for gamma-ray spectral analysis,as measured using a scintillation detector.A locally constrained regularization method was proposed to determine the resolution calibration para...Energy resolution calibration is crucial for gamma-ray spectral analysis,as measured using a scintillation detector.A locally constrained regularization method was proposed to determine the resolution calibration parameters.First,a Monte Carlo simulation model consistent with an actual measurement system was constructed to obtain the energy deposition distribution in the scintillation crystal.Subsequently,the regularization objective function is established based on weighted least squares and additional constraints.Additional constraints were designed using a special weighting scheme based on the incident gamma-ray energies.Subsequently,an intelligent algorithm was introduced to search for the optimal resolution calibration parameters by minimizing the objective function.The most appropriate regularization parameter was determined through mathematical experiments.When the regularization parameter was 30,the calibrated results exhibited the minimum RMSE.Simulations and test pit experiments were conducted to verify the performance of the proposed method.The simulation results demonstrate that the proposed algorithm can determine resolution calibration parameters more accurately than the traditional weighted least squares,and the test pit experimental results show that the R-squares between the calibrated and measured spectra are larger than 0.99.The accurate resolution calibration parameters determined by the proposed method lay the foundation for gamma-ray spectral processing and simulation benchmarking.展开更多
This study explores the influence of rotor blade angle on stall inception in an axial fan by means of numerical simulations grounded in the Reynolds-Averaged Navier-Stokes(RANS)equations and the Realizable k-εturbule...This study explores the influence of rotor blade angle on stall inception in an axial fan by means of numerical simulations grounded in the Reynolds-Averaged Navier-Stokes(RANS)equations and the Realizable k-εturbulence model.By analyzing the temporal behavior of the outlet static pressure,along with the propagation velocity of stall inception,the research identifies distinct patterns in the development of stall.The results reveal that stall inception originates in the second rotor impeller.At a blade angle of 27°,the stall inception follows a modal wave pattern,while in all other cases,it assumes the form of spike-type stall.The flow field associated with spike stall inception demonstrates a relatively uniform gradient in the radial direction,whereas the modal wave stall case displays a distinctive“L”-shaped propagation feature.At blade angles of multiple stall inceptions are observed.-9°and-18°,These phenomena initiate at the blade’s leading edge,propagate along both axial and radial directions,and transition dynamically between single and multiple inception states.展开更多
The longitudinal cracks distributed along the dam axis in the corridor of a dam may have potential safety hazards.According to the detection results of crack depth and width and the analysis of monitoring data,a three...The longitudinal cracks distributed along the dam axis in the corridor of a dam may have potential safety hazards.According to the detection results of crack depth and width and the analysis of monitoring data,a three-dimensional finite element model is established for numerical simulation calculation and the influence of cracks on the safety of dam structure is analyzed from different aspects such as deformation,stress value,and distribution range.The calculation results show that the maximum principal tensile stress value and the location of the dam body are basically independent of the change of crack depth(within 1.0 m).Regarding local stress around the corridor,the high upstream water level causes cracks to deepen,resulting in an increase in the maximum tensile stress near the crack tip and an expansion of the tensile stress region.展开更多
The inflation tests of rubbery membranes have been widely employed as an efficient method to characterize the stress response as biaxial loading states.However,most of the previous theoretical works have employed clas...The inflation tests of rubbery membranes have been widely employed as an efficient method to characterize the stress response as biaxial loading states.However,most of the previous theoretical works have employed classic hyperelastic models to analyze the deformation behaviors of inflated membranes.The classic models have been demonstrated to lack the ability to capturing the biaxial deformation of rubbers.To address this issue,we have combined the analytical method and the finite element simulation to investigate the deformation response of soft membranes with different constitutive relationships.For the analytical method,the governing ordinary differential equations have been set up for the boundary value problem of inflation tests and further solved using the shooting method.The analytical results are consistent with those obtained from finite element simulation.The results show that the deformation belongs to the unequal biaxial condition rather than the equi-biaxial state unless a neo-Hookean model is adopted.We also perform a parameter study using the extended eight-chain model,which shows that a change in different parameters affects the mechanical response of inflation tests variously.This work may shed light on the future experimental characterization of soft materials using inflation experiments.展开更多
Background:Epidemiological studies have confirmed that longer exposure to insecticides like cypermethrin(CYP)significantly increases the risk of male reproductive toxicity.Crocus sativus L.has been recognized due to i...Background:Epidemiological studies have confirmed that longer exposure to insecticides like cypermethrin(CYP)significantly increases the risk of male reproductive toxicity.Crocus sativus L.has been recognized due to its therapeutic properties,but its exact role and molecular mechanisms in treatment of reproductive dysfunction remain unclear.Methods:During this study,36 rats were randomly divided into six groups(n=6):control,CYP-induced(60 mg/kg),standard(leuprolide 3 mg/kg)and three treatment groups receiving aqueous,ethanolic,and oil extracts(50 mg/kg or 20 mL/kg)for post-toxicity induction.Results:The finding represented that exposure of CYP significantly increased oxidative stress,disrupted testicular architecture,and markedly reduced testosterone levels(P<0.05).Importantly,Crocus sativus L.treatment alleviated these changes by increasing the expression of Nrf2(nuclear factor erythroid 2-related factor 2),restoring the activity of antioxidant enzymes,and enhancing testicular histomorphology.Surprisingly,molecular docking established a high binding affinity of Crocus sativus L.phytoconstituents such as gallic acid,cinnamic acid and quercetin to the Nrf2-Keap1 complex.It is worth noting that,Crocus sativus L.exhibited a high level of protection against reproductive toxicity caused by CYP in male rats,which was mediated by the activation of Nrf2 pathway,reduction of oxidative damage,and favorable ADMET characteristics.Conclusion:Notably,this research provides a more valid,safe,and effective method of developing new drugs for reproductive disorders,however,further investigation is needed to support the research findings and implement it in clinical practice.展开更多
The design of counter-rotating turbine is one of new techniques to improve the thrust-weight ratio of jet propulsion engines.Numerical analysis of a low pressure（LP）counter-rotating turbine rotor blade is presented ...The design of counter-rotating turbine is one of new techniques to improve the thrust-weight ratio of jet propulsion engines.Numerical analysis of a low pressure（LP）counter-rotating turbine rotor blade is presented by using ANSYS/CFX software.Interaction of aerodynamics and solid mechanics coupling in the computation is applied.In some rating of turbine,stress distribution and vibration characteristics of low pressure turbine（LPT）blade are computed.The wake aerodynamic forces and LPT blade vibration are transformed in frequency domain using fast Fourier transform（FFT）method.The results show that under wake aerodynamic force excitation,the first order modal vibration is more easily aroused and the higher order response cannot be ignored.Moreover,with different temperature fields,the vibration responses of blade are also different.展开更多
Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macro...Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment.展开更多
基金funding support from the National Natural Science Foundation of China(Grant Nos.52208355and 52378308)Shenzhen University's 2035 Program for Excellent Research(Grant No.00000219).
文摘With the development of urban infrastructure,it is inevitable that shield tunnels will undercross intercity railways.However,the safe operation of intercity railways requires strict subgrade deformation.On the basis of the engineering background of the Lianghu Tunnel in Wuhan,the three-dimensional centrifuge test and numerical back analysis were used to study the development of subgrade surface settlement during shield tunneling.A three-dimensional numerical model with the same size as the prototype was subsequently established to further study the settlement development and torsion behavior of the subgrade during tunnel excavation.The results show that the maximum settlement point of the transverse settlement trough gradually moves to the tunnel axis during tunnel excavation and that the entire subgrade experiences torsional deformation.Moreover,the effect of the intersection angle between the axes of the tunnel and the subgrade on the surface settlement of the subgrade was further studied.The results show that the intersection angle has no effect on the maximum settlement,but the width of the settlement trough increases gradually with increasing angle.Finally,on the basis of the soil arching effect caused by tunnel excavation,the subgrade settlement during tunnel excavation is reduced by reinforcing the soil in different zones of soil arching.The results show that the settlement of the subgrade caused by the shield tunnel can be effectively controlled by adding reinforcement directly to the top of the tunnel,and the maximum settlement of the subgrade surface is reduced from 24.41 mm to 9.47 mm,a reduction of approximately 61.2%.
文摘A full-scale research study was conducted during the bored tunnelling of the Klang Valley Mass Rapid Transit-Putrajaya Line beneath an existing building structure in Kuala Lumpur,Malaysia.The primary objective was to investigate the tunnel-soil-pile interaction at various stages of tunnel excavation.This study combined field measurements and three-dimensional(3D)numerical analysis to understand the transient effects of TBM tunnelling on a loaded pile.An experimental pile was instrumented with vibrating wire strain gauges,an inclinometer,and distributed fibre optic sensors using Brillouin optical time domain analysis.The pile was pre-loaded and continuously monitored in real-time throughout the tunnel construction process.The 3D finite element modelling was used to simulate the pile’s transient responses based on actual tunnel boring machine(TBM)driving data.The study revealed that the zone of influence due to tunnelling effects extended from y¼2D to y¼4D,with the peak effect observed at y¼1D to 1.5D,where D represents the tunnel diameter.The analysis of axial load patterns highlighted transient responses,including tensile loads below the tunnel invert,which propagated upward and subsided due to negative skin friction.The maximum downdrag load observed reached 56%e71%of the pile’s working load.Additionally,pile movement patterns indicated outward deflections as the TBM approached and a return toward the tunnel post-passage,aligning with the predicted behaviour in a negative face loss scenario.This validated numerical framework provides a solid foundation for further parametric studies and enhances the understanding of tunnel-soil-pile interactions.
基金the financial support from the Fujian Science Foundation for Outstanding Youth(2023J06039)the National Natural Science Foundation of China(Grant No.41977259,U2005205,41972268)the Independent Research Project of Technology Innovation Center for Monitoring and Restoration Engineering of Ecological Fragile Zone in Southeast China(KY-090000-04-2022-019)。
文摘Shotcrete is one of the common solutions for shallow sliding.It works by forming a protective layer with high strength and cementing the loose soil particles on the slope surface to prevent shallow sliding.However,the solidification time of conventional cement paste is long when shotcrete is used to treat cohesionless soil landslide.The idea of reinforcing slope with polyurethane solidified soil(i.e.,mixture of polyurethane and sand)was proposed.Model tests and finite element analysis were carried out to study the effectiveness of the proposed new method on the emergency treatment of cohesionless soil landslide.Surcharge loading on the crest of the slope was applied step by step until landslide was triggered so as to test and compare the stability and bearing capacity of slope models with different conditions.The simulated slope displacements were relatively close to the measured results,and the simulated slope deformation characteristics were in good agreement with the observed phenomena,which verifies the accuracy of the numerical method.Under the condition of surcharge loading on the crest of the slope,the unreinforced slope slid when the surcharge loading exceeded 30 k Pa,which presented a failure mode of local instability and collapse at the shallow layer of slope top.The reinforced slope remained stable even when the surcharge loading reached 48 k Pa.The displacement of the reinforced slope was reduced by more than 95%.Overall,this study verifies the effectiveness of polyurethane in the emergency treatment of cohesionless soil landslide and should have broad application prospects in the field of geological disasters concerning the safety of people's live.
文摘Heat treatment processes, such as annealing and quenching, are crucial in determining residual stress evolution, microstructural changes and mechanical properties of metallic materials, with residual stresses playing a greater role in the performance of components. This paper investigates the effect of heat treatment on residual stresses induced in AISI 1025, manufactured using LENS. Finite element model was developed and simulated to analyze residual stress development. AISI 1025 samples suitable for tool and die applications in Fused Deposition Modelling (FDM) filament production, were fabricated using Laser Engineered Net Shaping (LENS) process, followed by heat treatment where annealing and quenching processes were done. The material’s microstructure, residual stress and hardness of heat-treated samples under investigation, were compared against the as-built samples. The results indicated that after annealing, tensile residual stresses were reduced by 93%, resulting in a reduced crack growth rate, compared to the as-built sample, although the hardness was reduced significantly by 25%. On the other hand, high tensile residual stresses of 425 ± 14 MPa were recorded after quenching process with an improvement of hardness by 21%.
基金supported by grants received by the first author and third author from the Institute of Eminence,Delhi University,Delhi,India,as part of the Faculty Research Program via Ref.No./IoE/2024-25/12/FRP.
文摘Software systems are vulnerable to security breaches as they expand in complexity and functionality.The confidentiality,integrity,and availability of data are gravely threatened by flaws in a system’s design,implementation,or configuration.To guarantee the durability&robustness of the software,vulnerability identification and fixation have become crucial areas of focus for developers,cybersecurity experts and industries.This paper presents a thorough multi-phase mathematical model for efficient patch management and vulnerability detection.To uniquely model these processes,the model incorporated the notion of the learning phenomenon in describing vulnerability fixation using a logistic learning function.Furthermore,the authors have used numerical methods to approximate the solution of the proposed framework where an analytical solution is difficult to attain.The suggested systematic architecture has been demonstrated through statistical analysis using patch datasets,which offers a solid basis for the research conclusions.According to computational research,learning dynamics improves security response and results in more effective vulnerability management.The suggested model offers a systematic approach to proactive vulnerability mitigation and has important uses in risk assessment,software maintenance,and cybersecurity.This study helps create more robust software systems by increasing patch management effectiveness,which benefits developers,cybersecurity experts,and sectors looking to reduce security threats in a growing digital world.
基金funded by the National Natural Science Foundation of China(Nos.42077228,52174085)。
文摘With the increasing development of deepburied engineering projects,rockburst disasters have become a frequent concern.Studies have indicated that tunnel diameter is a critical factor influencing the occurrence of rockbursts.To investigate the influence of tunnel diameter on the deformation and failure characteristics of surrounding rock,large-sized rocklike gypsum specimens were tested using a selfdeveloped true triaxial rockburst loading system containing circular tunnels with three different diameters(D=0.07 m,0.11 m,and 0.15 m).Acoustic emission monitoring,together with a miniature intelligent camera,was employed to analyze the entire process,focusing on macroscopic failure patterns,fragment characteristics,and underlying failure mechanisms.In addition,theoretical analyses were carried out and combined with numerical simulations to investigate the differences in energy evolution associated with rockburst physical models.The results indicate that:(1)The rockburst process with different tunnel diameters consistently evolved through three distinct stages—initial particle ejection,crack propagation accompanied by flake spalling,and,finally,fragment ejection leading to the formation of a‘V'-shaped notch.(2)Increasing tunnel diameter reduces rockburst failure load while increasing surrounding rock damage extent,total mass and average size of ejected fragments.Additionally,shear failure proportion decreases with tensile failure becoming increasingly dominant.(3)Larger tunnel diameters reduce the attenuation rate of elastic strain energy,thereby expanding the zone of elastic strain energy accumulation and disturbance and creating conditions for larger volume rockburst.(4)Larger tunnel diameters result in a smaller principal stress ratio at equivalent distances in the surrounding rock,indicating a higher likelihood of tensile failure.(5)Numerical analyses further reveal that larger tunnel diameters reduce the maximum elastic strain energy density around the tunnel,lowering the energy released per unit volume of rockburst fragments and their ejection velocities.However,both the total failure volume and overall energy release from rockburst increase.Model experiments with different tunnel diameters are of great significance for optimizing engineering design and parameter selection,as well as guiding tunnel construction under complex geological conditions.
基金supported by the Young Scientists Fund of National Natural Science Foundation of China(Grant Nos.12202202 and 12202494)the National Key Research and Development Program of China(Grant No.2021YFC3100700)。
文摘The internal and external flow fields during vented explosions of methane were characterized through numerical simulation,and the capability of numerical simulation thereof was validated by previous experimental data at three ignition positions.The venting mechanism was revealed by the simulated concentration distribution,temperature profile,and airflow velocity.The results show rear ignition results in the external methane mass distribution taking the form of"mushroom"and columnar flames in the external space,which can be expressed as a third-order polynomial relationship with distance;central ignition forms a relationship of the form y=AxB.Front ignition causes the temperature to show a tendency to repeated oscillations(rising,falling,and rising).Central ignition generates the maximum vented airflow velocity(V_(max)=320 m/s)upon vent opening.The results indicate that it is acceptable to apply numerical simulation of methane explosions in practice.
基金funding from the National Natural Science Foundation of China(No.12462028).
文摘This study employs the Smoothed Particle Hydrodynamics(SPH)method to develop a computational fluid dynamics(CFD)model for analyzing the interaction between rogue waves and mooring systems.Four floating body configurations are investigated:(1)dual rectangular prisms,(2)rectangular prism–sphere composites,(3)sphere–rectangular prism composites,and(4)dual spheres.These configurations are systematically evaluated under varying mooring conditions to assess their hydrodynamic performance and wave attenuation capabilities.The model accurately captures the complex fluid–structure interaction dynamics between moored floating breakwaters and incident wave fields.Among the configurations,the dual rectangular prism system demonstrates superior performance in both wave dissipation and mooring force reduction.Under conditions involving dual wave makers,the influence of floating body shape and number on wave height is found to be minimal.However,dual-body arrangements consistently outperform single-body setups in terms of both energy dissipation and structural stability.From a cost-efficiency perspective,the configuration comprising two rectangular prisms connected via a single mooring system offers significant advantages in material usage and deployment feasibility.
基金supported by the Beijing Postdoctoral Research Foundation(No.2023-ZZ-133)Scientific Research Foundation of Beijing Infrastructure Investment Co.,Ltd.(No.2023-ZB-03)Fundamental Research Funds for the Central Universities(No.2682023ZTPY036).
文摘The pressure wave generated by an urban-rail vehicle when passing through a tunnel affects the comfort of passengersand may even cause damage to the train and related tunnel structures.Therefore,controlling the trainspeed in the tunnel is extremely important.In this study,this problem is investigated numerically in the frameworkof the standard k-εtwo-equation turbulence model.In particular,an eight-car urban rail train passingthrough a tunnel at different speeds(140,160,180 and 200 km/h)is considered.The results show that the maximumaerodynamic drag of the head and tail cars is most affected by the running speed.The pressure at selectedmeasuring points on the windward side of the head car is very high,and the negative pressure at the side windowof the driver’s cab of the tail car is also very large.From the head car to the tail car,the pressure at the same heightgradually decreases.The positive pressure peak at the head car and the negative pressure peak at the tail car aregreatly affected by the speed.
文摘Enhancing the fermentation efficiency of waste in waste warehouses is pivotal for accelerating the pyrolysis process and minimizing harmful gas emissions.This study proposes an integrated approach,combining hot air injection with dual atomizing nozzles,for the thermal treatment of waste piles.Numerical simulations are employed to investigate the influence of various parameters,namely,nozzle height,nozzle tilt angle,inlet air velocity and air temperature,on the droplet diffusion process,spread area,droplet temperature,and droplet size distribution.The results show that reducing the nozzle height increases the temperature of droplets upon their deposition on the waste pile.Specifically,when the nozzle height is lowered to 1.5 m,the temperature of the droplets reaching the waste pile is 1℃higher than when the nozzle height is set at 2 m.Furthermore,an increase in the nozzle tilt angle expands the overlapping heating area.For instance,when the nozzle angle is increased from 15°to 30°,the overlapping spread area expands by 3.21 m2.Additionally,increasing the inlet air velocity enhances the droplet diffusion range.At an air velocity of 2 m/s,the droplet diffusion range grows to 14.4 m,representing a 6.7%increase compared to the nowind condition.While the average droplet diameter decreases to 1.53 mm,the droplet temperature decreases by 1℃.Moreover,the droplet temperature is found to become smaller as the ambient temperature inside the waste warehouse declines.Specifically,a 5℃reduction in the ambient temperature results in a 1℃decrease in the average temperature of the atomized droplets.The study concludes that a nozzle height of 1.5 m and a nozzle tilt angle of 30°effectively meet practical heating requirements.
基金supported by the China Scholarship Council(CSC)(Grant No.202006060033).
文摘Both the complex geometrical morphology of rough-walled rock fractures and the nonlinearity of fluid flow contribute to resistance in fluid flow through rock fractures.The interactions of the shear-flow process further complicate the characterisation of flow behaviours in rock fractures.In this study,an improved friction factor model involving both the effects of viscous and inertial forces is presented based on the Forchheimer equation.The model incorporates two key variables,i.e.Reynolds number and relative roughness,which reflect the effects of flow regimes and fracture roughness,respectively.The changes in geometrical parameters induced by shearing are considered,with the peak asperity height predicted through a correlation with post-peak roughness degradation.The hydraulic aperture during shearing is estimated using a suggested equation that accounts for the mobilised contact area ratio and variable aperture distribution.The parametric sensitivity analysis reveals that shear-induced changes in fracture geometry enhance the flow nonlinearity in rock fractures.The model performs well in predicting the friction factor based on two validation criteria.Then,the proposed friction factor model is incorporated into the three-dimensional distinct element code(3DEC)in the form of the Darcy-Weisbach equation.Coupled with the numerically implemented mechanical model and hydraulic aperture prediction model,numerical simulations of coupled shear-flow processes in single rock fractures are conducted.The simulation outcomes are validated through comparison with the experimental results,showing acceptable agreement and demonstrating that the numerical model is capable of accurately evaluating the hydro-mechanical coupling behaviour during the shearing of rock fractures.
基金supported by the National Natural Science Foundation of China(No.52204026).
文摘Produced water reinjection is a common strategy in offshore oilfield operations,yet the presence of solid particles in produced water can lead to localized formation pressure buildup,increasing the risk of rock fracturing and leakage.In this study,we present an integrated experimental and numerical investigation to quantify the effects of particle migration on formation pressure and the spatial diffusion of injected water.Dynamic plugging experiments were performed to systematically examine the influence of injection rate and injection volume on core permeability.Results demonstrate that higher injection rates substantially reduce permeability,and the derived relationship between permeability and injection volume enables dynamic assessment of permeability evolution during reinjection.Complementary numerical simulations explored the impacts of injection length,particle concentration,and injection rate on formation pressure and diffusion behavior.Findings indicate that extending the injection section promotes pressure distribution and enlarges the diffusion area,whereas elevated particle concentrations and injection rates accelerate formation plugging,causing rapid pressure rise and constrained diffusion.
基金The National Natural Science Foundation of China(No.52278189)Zhejiang Provincial Natural Science Foundation of China(No.LY24E080002).
文摘A self-centering bridge bent equipped with energy-dissipation(ED)beams is proposed.Quasi-static tests are conducted on self-centering bridge bents,both with and without ED beams,to validate the accuracy of the corresponding numerical models.The effects of various param-eters,such as the web area of ED beams,prestressing force of tendons,tendon arrangements,and number of column segments,on the seismic performance of self-centering bridge bents with ED beams are evaluated using the validated numerical model.The results demonstrate that the nu-merical models accurately replicate the quasi-static test results,with average errors in the lateral force remaining below 9.6%.The web area of ED beams significantly affects the strength,cumulative energy dissipation,and relative self-centering index(RSI)of the self-centering bridge bents.Increasing the prestressing force enhances the lateral force and self-centering capability of the bridge bents but has minimal effect on their ED capacity.Reducing the num-ber of segments in each column enhances the lateral force and cumulative hysteretic energy dissipation of the self-centering bridge bents while exerting an insignificant effect on the RSI.Thus,the proposed novel system is highly suitable for doubleor multicolumn piers supporting bridges in regions prone to strong earthquakes.
基金supported by the National Natural Science Foundation of China(No.41804141)。
文摘Energy resolution calibration is crucial for gamma-ray spectral analysis,as measured using a scintillation detector.A locally constrained regularization method was proposed to determine the resolution calibration parameters.First,a Monte Carlo simulation model consistent with an actual measurement system was constructed to obtain the energy deposition distribution in the scintillation crystal.Subsequently,the regularization objective function is established based on weighted least squares and additional constraints.Additional constraints were designed using a special weighting scheme based on the incident gamma-ray energies.Subsequently,an intelligent algorithm was introduced to search for the optimal resolution calibration parameters by minimizing the objective function.The most appropriate regularization parameter was determined through mathematical experiments.When the regularization parameter was 30,the calibrated results exhibited the minimum RMSE.Simulations and test pit experiments were conducted to verify the performance of the proposed method.The simulation results demonstrate that the proposed algorithm can determine resolution calibration parameters more accurately than the traditional weighted least squares,and the test pit experimental results show that the R-squares between the calibrated and measured spectra are larger than 0.99.The accurate resolution calibration parameters determined by the proposed method lay the foundation for gamma-ray spectral processing and simulation benchmarking.
基金the Natural Science Foundation of Hebei Province,China(Grant No.E2022502052)Fundamental Research Funds for the Central Universities,China(Grant No.2022MS081)Fundamental Research Funds for the Central Universities,China(Grant No.2023MS121).
文摘This study explores the influence of rotor blade angle on stall inception in an axial fan by means of numerical simulations grounded in the Reynolds-Averaged Navier-Stokes(RANS)equations and the Realizable k-εturbulence model.By analyzing the temporal behavior of the outlet static pressure,along with the propagation velocity of stall inception,the research identifies distinct patterns in the development of stall.The results reveal that stall inception originates in the second rotor impeller.At a blade angle of 27°,the stall inception follows a modal wave pattern,while in all other cases,it assumes the form of spike-type stall.The flow field associated with spike stall inception demonstrates a relatively uniform gradient in the radial direction,whereas the modal wave stall case displays a distinctive“L”-shaped propagation feature.At blade angles of multiple stall inceptions are observed.-9°and-18°,These phenomena initiate at the blade’s leading edge,propagate along both axial and radial directions,and transition dynamically between single and multiple inception states.
基金Zhejiang Provincial Natural Science Foundation of China for Young Scholars(Project No.:LQ20A020009)National College Students’Innovation and Entrepreneurship Training Program(Project No.:202311842014X)。
文摘The longitudinal cracks distributed along the dam axis in the corridor of a dam may have potential safety hazards.According to the detection results of crack depth and width and the analysis of monitoring data,a three-dimensional finite element model is established for numerical simulation calculation and the influence of cracks on the safety of dam structure is analyzed from different aspects such as deformation,stress value,and distribution range.The calculation results show that the maximum principal tensile stress value and the location of the dam body are basically independent of the change of crack depth(within 1.0 m).Regarding local stress around the corridor,the high upstream water level causes cracks to deepen,resulting in an increase in the maximum tensile stress near the crack tip and an expansion of the tensile stress region.
基金supported by the National Natural Science Foundation of China(Grant Nos.12211530061 and 12321002)the Zhejiang Provincial Natural Science Foundation of China(Grant No.LD22A020001)the 111 Project(Grant No.B21034).
文摘The inflation tests of rubbery membranes have been widely employed as an efficient method to characterize the stress response as biaxial loading states.However,most of the previous theoretical works have employed classic hyperelastic models to analyze the deformation behaviors of inflated membranes.The classic models have been demonstrated to lack the ability to capturing the biaxial deformation of rubbers.To address this issue,we have combined the analytical method and the finite element simulation to investigate the deformation response of soft membranes with different constitutive relationships.For the analytical method,the governing ordinary differential equations have been set up for the boundary value problem of inflation tests and further solved using the shooting method.The analytical results are consistent with those obtained from finite element simulation.The results show that the deformation belongs to the unequal biaxial condition rather than the equi-biaxial state unless a neo-Hookean model is adopted.We also perform a parameter study using the extended eight-chain model,which shows that a change in different parameters affects the mechanical response of inflation tests variously.This work may shed light on the future experimental characterization of soft materials using inflation experiments.
文摘Background:Epidemiological studies have confirmed that longer exposure to insecticides like cypermethrin(CYP)significantly increases the risk of male reproductive toxicity.Crocus sativus L.has been recognized due to its therapeutic properties,but its exact role and molecular mechanisms in treatment of reproductive dysfunction remain unclear.Methods:During this study,36 rats were randomly divided into six groups(n=6):control,CYP-induced(60 mg/kg),standard(leuprolide 3 mg/kg)and three treatment groups receiving aqueous,ethanolic,and oil extracts(50 mg/kg or 20 mL/kg)for post-toxicity induction.Results:The finding represented that exposure of CYP significantly increased oxidative stress,disrupted testicular architecture,and markedly reduced testosterone levels(P<0.05).Importantly,Crocus sativus L.treatment alleviated these changes by increasing the expression of Nrf2(nuclear factor erythroid 2-related factor 2),restoring the activity of antioxidant enzymes,and enhancing testicular histomorphology.Surprisingly,molecular docking established a high binding affinity of Crocus sativus L.phytoconstituents such as gallic acid,cinnamic acid and quercetin to the Nrf2-Keap1 complex.It is worth noting that,Crocus sativus L.exhibited a high level of protection against reproductive toxicity caused by CYP in male rats,which was mediated by the activation of Nrf2 pathway,reduction of oxidative damage,and favorable ADMET characteristics.Conclusion:Notably,this research provides a more valid,safe,and effective method of developing new drugs for reproductive disorders,however,further investigation is needed to support the research findings and implement it in clinical practice.
文摘The design of counter-rotating turbine is one of new techniques to improve the thrust-weight ratio of jet propulsion engines.Numerical analysis of a low pressure（LP）counter-rotating turbine rotor blade is presented by using ANSYS/CFX software.Interaction of aerodynamics and solid mechanics coupling in the computation is applied.In some rating of turbine,stress distribution and vibration characteristics of low pressure turbine（LPT）blade are computed.The wake aerodynamic forces and LPT blade vibration are transformed in frequency domain using fast Fourier transform（FFT）method.The results show that under wake aerodynamic force excitation,the first order modal vibration is more easily aroused and the higher order response cannot be ignored.Moreover,with different temperature fields,the vibration responses of blade are also different.
基金supported by Qingdao Key Medical and Health Discipline ProjectThe Intramural Research Program of the Affiliated Hospital of Qingdao University,No. 4910Qingdao West Coast New Area Science and Technology Project,No. 2020-55 (all to SW)。
文摘Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment.
