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Controllable Fabrication of Spider-Web-Like Structured Anaphe Panda Regenerated Silk Nanofibers / Nets via Electrospinning / Netting 被引量:5
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作者 DULO Benson 王娜 +3 位作者 李彦 OYONDI Erick MADARA S Diana 丁彬 《Journal of Donghua University(English Edition)》 EI CAS 2014年第4期497-502,共6页
An Anaphe panda silk nanofibers / nets( NFN) membrane with attractive structures consisting of common electrospun nanofibers and two-dimensional( 2D) spider-web-like structured nano-nets were successfully fabricated v... An Anaphe panda silk nanofibers / nets( NFN) membrane with attractive structures consisting of common electrospun nanofibers and two-dimensional( 2D) spider-web-like structured nano-nets were successfully fabricated via electrospinning / netting technology. The unique structures of NFN membranes such as extremely small diameter( < 20 nm),high porosity,large specific surface area. and biocompatibility make this Anaphe panda silk NFN membrane a promising candidate for biomedical applications. In the present study, field emission scanning electron microscopy( FESEM) was used to investigate the influence of polymer solution and humidity on nano-nets coverage and morphology. The FE-SEM images revealed that nano-net coverage area increased with increase in concentration of solution while lowering the humidity increased the nano-nets formation. Moreover,the mechanical properties of the membrane were also tested and the result showed that the silk NFN membrane displayed a breaking stress of 3. 7 MPa and breaking strain of 13. 8%. For further structural elucidation, Fourier transform infrared spectroscopy( FT-IR) was used to analyze the structural conformation changes from random coil to β-sheet in the NFN membrane which was an important factor effecting the usability of membrane. Thus,the results above confirmed the feasibility of Anaphe panda NFN structures applicability in cell tissue culture and other biomedical applications. 展开更多
关键词 Anaphe panda silk electrospinning / netting nanofibers / nets(NFN) biocompatibility
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Impacts of bird abundance, activity height and light intensity on the number of birds captured by mist netting 被引量:2
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作者 邹发生 陈桂珠 杨琼芳 《Chinese Birds》 2010年第4期221-229,共9页
To understand better the impact on mist net capture rates of the activity height of birds, light intensity, bird richness and abundance, we compared data from mist nets and point counts at the Jianfengling Forest Area... To understand better the impact on mist net capture rates of the activity height of birds, light intensity, bird richness and abundance, we compared data from mist nets and point counts at the Jianfengling Forest Area, Hainan Island. Mist-nets were operated for a total of 7135 net-hours. A total of 587 individuals of 45 bird species were captured. The total mean capture rate was 8.6 ± 10.4 per 100 net-hours. A total of 4932 individuals of 107 bird species were recorded by visual observation alone and 7616 individuals of 120 species were recorded in a visual and aural combination. The Grey-cheeked Fulvetta (Alcippe morrisonia) was the most abundant species, accounting for 38.7% of total captures, 27.9% of visual observations and 22.2% of visual + aural observations. The capture number was correlated with the number observed. Thirty-one species were more likely to be captured than observed. Canopy species, such as members of the Corvidae and Dicruridae, were seldom captured. For all ground feeding species, the capture number was greater than that of observation. Some cryptic species, such as the Lesser Wren Babbler (Napothera epilepidota), Spotnecked Babbler (Stachyris striolata) and Cuckoo Owl (Glaucidium cuculoides) also had a higher relative capture rate than that of observations. There was a significant relationship between light intensity and the number of birds captured. 展开更多
关键词 activity height bird abundance light intensity mist nets point counts
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Experimental and Numerical Investigations of the Hydrodynamic Characteristics, Twine Deformation, and Flow Field Around the Netting Structure Composed of Two Types of Twine Materials for Midwater Trawls
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作者 THIERRY Bruno Nyatchouba Nsangue TANG Hao +3 位作者 ACHILE Njomoue Pandong XU Liuxiong ZHOU Cheng HU Fuxiang 《Journal of Ocean University of China》 SCIE CAS CSCD 2021年第5期1215-1235,共21页
Nettings are complex flexible structures used in various fisheries.Understanding the hydrodynamic characteristics,de-formation,and the flow field around nettings is important to design successful fishing gear.This stu... Nettings are complex flexible structures used in various fisheries.Understanding the hydrodynamic characteristics,de-formation,and the flow field around nettings is important to design successful fishing gear.This study investigated the hydrodynamic characteristics and deformation of five nettings made of polyethylene and nylon materials in different attack angles through numeri-cal simulation and physical model experiment.The numerical model was based on the one-way coupling between computational fluid dynamics(CFD)and large deflection nonlinear structural models.Navier-Stokes equations were solved using the finite volume ap-proach,the flow was described using the k-ωshear stress turbulent model,and the large deflection structural dynamic equation was derived using a finite element approach to understand the netting deformation and nodal displacement.The porous media model was chosen to model the nettings in the CFD solver.Numerical data were compared with the experimental results of the physical model to validate the numerical models.Results showed that the numerical data were compatible with the experimental data with an average relative error of 2.34%,3.40%,6.50%,and 5.80%in the normal drag coefficients,parallel drag coefficients,inclined drag coefficients,and inclined lift coefficients,respectively.The hydrodynamic forces of the polyethylene and nylon nettings decreased by approxi-mately 52.56%and 66.66%,respectively,with decreasing net solidity.The drag and lift coefficients of the nylon netting were appro-ximately 17.15%and 6.72%lower than those of the polyethylene netting.A spatial development of turbulent flow occurred around the netting because of the netting wake.However,the flow velocity reduction downstream from the netting in the wake region in-creased with increasing attack angle and net solidity.In addition,the deformation,stress,and strain on each netting increased with in-creasing solidity ratio. 展开更多
关键词 netting k-ωshear stress turbulent(SST)model porous media model large deflection nonlinear structural model hydrodynamic characteristics
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Experimental Modelling of Transverse Oscillations in Aquaculture Netting Parallel to the Flow – Sounds Baffling
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作者 Andrew JOHNSON Cheslav BALASH 《China Ocean Engineering》 SCIE EI CSCD 2015年第3期391-400,共10页
Numerous studies have been undertaken to improve the viability, durability and suitability of materials and methods used for aquaculture enclosures. While many of the previous studies considered macro-deformation of n... Numerous studies have been undertaken to improve the viability, durability and suitability of materials and methods used for aquaculture enclosures. While many of the previous studies considered macro-deformation of nets, there is a paucity of information on netting micro-deformation. When aquaculture pens are towed, industry operators have observed the motion described as "baffling" – the transverse oscillation of the net planes parallel and near parallel to the flow. The difficulty to observe and assess baffling motion in a controlled experimental environment is to sufficiently reproduce netting boundary conditions and the flow environment experienced at sea. The focus of the present study was to develop and assess experimental methods for visualisation and quantification of these transverse oscillations. Four netrig configurations with varied boundary conditions and model-netting properties were tested in a flume tank. While the Reynolds number was not equivalent to full-scale, usage of the pliable and fine mesh model netting that enabled baffling to develop at low flow velocities was deemed to be of a larger relevance to this initial study. Baffling was observed in the testing frame that constrained the net sheet on the leading edge, similarly to a flag attachment onto a pole. Baffling motion increased the hydrodynamic drag of the net by 35%–58% when compared to the previously developed formula for taut net sheets aligned parallel to the flow. Furthermore, it was found that the drag due to baffling decreased with the increasing velocity over the studied Reynolds numbers(below 200); and the drag coefficient was non-linear for Reynolds numbers below 120. It is hypothesised that baffling motion is initially propagated by vortex shedding of the netting twine which causes the netting to oscillate; there after the restoring force causes unstable pressure differences on each side of the netting which excites the amplitude of the netting oscillations. 展开更多
关键词 aquaculture pen baffling model net testing netting drag netting micro-deformation
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A comparison of hydrodynamic forces in knotted and knotless netting,using both helix and conventional ropes for midwater trawls
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作者 Gebremeskel Eshetu Kebede Paul D.Winger 《Aquaculture and Fisheries》 2021年第1期96-105,共10页
This study evaluated the hydrodynamic forces produced by whole panels of hand-made(geometrically different)rope meshes typically found in midwater trawls.Experimental treatments included both knotted and knotless mesh... This study evaluated the hydrodynamic forces produced by whole panels of hand-made(geometrically different)rope meshes typically found in midwater trawls.Experimental treatments included both knotted and knotless meshes constructed of helix composite ropes,conventional braided nylon(PA)ropes,and twisted polyethylene(PE)ropes.Direct measurement of lift(L)and drag(D)forces were conducted using controlled flume tank experiments on panels oriented at 21°relative to the flow.For knotted netting,our results showed that the hydrodynamic forces(L and D)were statistically higher for helix ropes compared to conventional ropes of the same diameter.For knotless netting constructed of helix ropes,our primary finding is that the arrangement of helix ropes(S and Z lay)in individual meshes significantly affects the resulting D and Cd of whole netting panels.Arranging the mesh bars in the S-Z-S-Z lay pattern exhibited 29%and 32%lower drag(D)compared to S–S–S–S and S–S-Z-Z lay meshes,respectively.We also use computational fluid dynamics(CFD)to characterize the finescale flow-field around helix and conventional ropes.The resulting streamlines and pressure contours provide a functional explanation for the empirical measurements collected in the flume tank.These results can be useful in informing the design of midwater trawls. 展开更多
关键词 Drag LIFT Helix rope Knotted netting Knotless netting Reynolds number
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膀胱癌细胞外泌体诱导中性粒细胞外诱捕网形成对肿瘤细胞增殖、迁移和侵袭的研究 被引量:1
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作者 符仕宝 陈宁 +3 位作者 何书明 杨杰 彭泽椿 徐明彬 《中国免疫学杂志》 北大核心 2025年第2期351-356,共6页
目的:探究膀胱癌细胞外泌体诱导中心粒细胞胞外诱捕网(NETs)而影响肿瘤细胞生长和转移的功能及机制。方法:提取SV-HUC-1、MGH-U3和253J细胞分泌的外泌体,并用PBS和上述外泌体与中性粒细胞(PMN)共孵育,分为PBS组、SV-HUC-1 exo组、MGH-U3... 目的:探究膀胱癌细胞外泌体诱导中心粒细胞胞外诱捕网(NETs)而影响肿瘤细胞生长和转移的功能及机制。方法:提取SV-HUC-1、MGH-U3和253J细胞分泌的外泌体,并用PBS和上述外泌体与中性粒细胞(PMN)共孵育,分为PBS组、SV-HUC-1 exo组、MGH-U3 exo组、253J exo组。Sytox green染色检测NETs的生成,Western blot检测NETs标志蛋白CIT-H3、NE、MPO表达。与MGH-U3和253J细胞分为Control组、PMN组、MGH-U3 exo组、253J exo组、MGH-U3 exo+PMN组、253J exo+PMN组。MGH-U3 exo组和253J exo组的PMN培养上清加入1.5 U/ml DNAse,共孵育30 min后,收集混合液分别与MGH-U3和253J细胞共孵育,记为MGH-U3 exo+PMN+DNAse组和253J exo+PMN+DNAse组。CCK-8法检测MGH-U3和253J细胞增殖能力,Transwell小室检测细胞迁移和侵袭,Western blot检测PI3K和AKT磷酸化水平。结果:与PBS组相比,MGH-U3 exo组和253J exo组NETs形成增加,CIT-H3、NE、MPO表达显著增加(P<0.001),SV-HUC-1 exo组的上述指标无显著变化。与Control组相比,MGH-U3 exo+PMN组和253J exo+PMN组细胞增殖、迁移和侵袭能力均显著增强(P<0.05),PI3K和AKT磷酸化水平均显著升高(P<0.001),相比于MGH-U3 exo+PMN组,MGH-U3 exo+PMN+DNAse组细胞增殖、迁移和侵袭能力显著下降(P<0.05),PI3K和AKT磷酸化水平均显著降低(P<0.001),相比于253J exo+PMN组,253J exo+PMN+DNAse组细胞增殖、迁移和侵袭能力显著下降(P<0.05),PI3K和AKT磷酸化水平均显著降低(P<0.001)。结论:膀胱癌细胞外泌体可通过诱导NETs的生成而促进肿瘤细胞的增殖、迁移和侵袭能力。 展开更多
关键词 膀胱癌 外泌体 NETS 增殖 迁移 侵袭
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中性粒细胞在真菌性角膜炎中的作用 被引量:1
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作者 杨珺铭 罗艳婷 +1 位作者 何宏 钟兴武 《国际眼科杂志》 2025年第2期230-234,共5页
真菌性角膜炎是一种高发的致盲性疾病,当前的治疗效果仍不理想。该疾病的发生和进展通常受到真菌毒力及宿主免疫反应的显著影响。在疾病进程中,先天性免疫反应首先被激活,其中中性粒细胞在抗真菌过程中发挥关键作用。虽然中性粒细胞在... 真菌性角膜炎是一种高发的致盲性疾病,当前的治疗效果仍不理想。该疾病的发生和进展通常受到真菌毒力及宿主免疫反应的显著影响。在疾病进程中,先天性免疫反应首先被激活,其中中性粒细胞在抗真菌过程中发挥关键作用。虽然中性粒细胞在清除病原体方面具有重要功能,但是其过度或异常激活可能引发组织损伤,从而加重病情。因此,探究中性粒细胞在真菌性角膜炎中的作用机制,对于优化治疗策略至关重要。文章旨在综述中性粒细胞的主要杀菌机制,包括吞噬作用、脱颗粒、中性粒细胞胞外诱捕网(NETs)的形成,并探讨中性粒细胞与巨噬细胞的相互作用,以及其在真菌性角膜炎中的效应及潜在机制。对于真菌性角膜炎机制探究,有利于施行的精准干预并增强其治疗效果。 展开更多
关键词 中性粒细胞 真菌性角膜炎 免疫 巨噬细胞 中性粒细胞胞外诱捕网(NETs)
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基于中性粒细胞胞外诱捕网探究补肾活血方对抗磷脂综合征的作用机制
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作者 徐佳 王巧 +1 位作者 付丽霞 陈瑾 《药物评价研究》 北大核心 2025年第9期2483-2493,共11页
目的探究补肾活血方(BSHX)在抗磷脂综合征(APS)模型中的作用及其对中性粒细胞胞外诱捕网(NETs)的调控机制。方法将小鼠随机分为5组:对照组、模型组、阿司匹林(阳性药,0.585 mg∙kg^(−1))组和BSHX低、高剂量(5.8、11.60 g∙kg^(−1))组。除... 目的探究补肾活血方(BSHX)在抗磷脂综合征(APS)模型中的作用及其对中性粒细胞胞外诱捕网(NETs)的调控机制。方法将小鼠随机分为5组:对照组、模型组、阿司匹林(阳性药,0.585 mg∙kg^(−1))组和BSHX低、高剂量(5.8、11.60 g∙kg^(−1))组。除对照组外,采用β2糖蛋白I(β2GP-I)构建APS小鼠模型,连续ig给药15 d,每天2次;通过ELISA法测定血清中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6表达水平;采用全自动凝血测试仪检测血浆中活化部分凝血活酶时间(APTT)、凝血酶时间(TT)和凝血酶原时间(PT)。体外培养人脐静脉内皮细胞(HUVECs),60μmol·L^(−1)β2GP-I刺激制备APS模型,同时给予不同浓度BSHX孵育24 h;MTT法检测BSHX(40、80、120、160、200、240、280、320μg·mL^(−1))对细胞存活率的影响;荧光成像结合流式细胞术检测BSHX(80、120μg·mL^(−1))对活性氧(ROS)水平的影响;Transwell实验检测BSHX(120μg·mL^(−1))对细胞迁移能力的影响;免疫荧光及试剂盒法检测NETs的形成;Western blotting检测BSHX(120μg·mL^(−1))对炎症相关(IL-6、TNF-α)、细胞黏附相关[细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)]、凝血功能相关[组织因子(TF)、纤溶酶原激活抑制剂-1(PAI-1)]、NADPH氧化酶2(NOX2)、p38、细胞外调节蛋白激酶(ERK)、c-Jun氨基末端激酶(JNK)蛋白表达水平;将HUVECs细胞分为对照组、模型组、BSHX(120μg·mL^(−1))组和MK-2206组(Akt抑制剂,100 nmol·L^(−1)),Western blotting检测ERK、p47、JNK、p38及磷酸化蛋白表达。结果与模型组比较,给予APS小鼠BSHX后,APTT、TT、PT均显著升高,TNF-α、IL-6表达水平被抑制(P<0.05、0.01、0.001),说明BSHX可缓解血液高凝状态、抗炎。与模型组比较,BSHX可显著改善APS模型HUVECs的细胞存活率,降低ROS水平和细胞迁移能力,抑制NETs的形成,抑制IL-6、TNF-α、ICAM-1、VCAM-1、TF、PAI-1、NOX2、p-JNK、p-ERK和p-p38蛋白表达(P<0.01、0.001);给予Akt通路抑制剂后p-ERK、p-p47、p-JNK、p-p38和NOX2蛋白表达显著降低(P<0.01、0.001)。结论补肾活血方可能通过调节Akt/ERK信号通路调控NETs的生成而发挥治疗APS作用。 展开更多
关键词 补肾活血方 抗磷脂综合征 HUVECS NETS Akt/ERK信号通路
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中性粒细胞胞外诱捕网在糖尿病创面中的作用与机制研究进展
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作者 张培深 武胡雯 +5 位作者 周涵 邓晗彬 范闻轩 李嘉诚 王溶 程少文 《海南医科大学学报》 北大核心 2025年第2期146-151,共6页
糖尿病创面是常见的慢性难愈性创面,常伴有持续炎症反应,其中中性粒细胞充当重要角色,通过调控炎症反应的进展,影响创面愈合。近年来,研究发现中性粒细胞胞外诱捕网(neutrophil extracellular traps,NETs)可通过参与机体免疫反应,充当... 糖尿病创面是常见的慢性难愈性创面,常伴有持续炎症反应,其中中性粒细胞充当重要角色,通过调控炎症反应的进展,影响创面愈合。近年来,研究发现中性粒细胞胞外诱捕网(neutrophil extracellular traps,NETs)可通过参与机体免疫反应,充当物理和抗菌屏障,杀灭炎症部位病原体,但其在糖尿病创面中持续过度存在可能导致创面愈合迟缓。因此,本文就NETs在糖尿病创面中的各种作用、机制及相关干预策略的研究进展进行综述。 展开更多
关键词 NETS 糖尿病 创面 作用 机制
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The deformation characteristics and flow field around knotless polyethylene netting based on fluid structure interaction(FSI)one-way coupling 被引量:4
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作者 Baiqiang Zou Nyatchouba Nsangue Bruno Thierry +3 位作者 Hao Tang Liuxiong Xu Shuchuang Dong Fuxiang Hu 《Aquaculture and Fisheries》 2022年第1期89-102,共14页
Knotless polyethylene(PE)netting is widely used in fisheries because of its excellent hydrodynamic performance and low cost.Netting deformation and the surrounding flow field distribution play an important role in det... Knotless polyethylene(PE)netting is widely used in fisheries because of its excellent hydrodynamic performance and low cost.Netting deformation and the surrounding flow field distribution play an important role in determining the hydrodynamic characteristics of netting in moving water.In order to investigate the effect of solidity ratio and attack angle on drag,netting deformation,and flow field distribution through the netting,a fluid-structure interaction(FSI)model based on a one-way coupling combining the shear stress turbulent(SST)k-omega model and the large deformation nonlinear structural finite element model was evaluated.Our results showed the difference between the parallel and normal drag forces found in the present numerical model and experimental flume tank data were 9.17%and 11.58%,respectively.The mean relative error in the inclined hydrodynamic drag for different flow velocities and attack angles was 8.35%,6.69%,and 5.37%for the nettings 1,2,and 3,respectively.These results show that the present numerical simulation based on FSI one-way coupling can be used to examine hydrodynamic forces on netting.The flow simulation results show that there is a noticeable flow velocity decrease through the netting and a rather large velocity reduction region downstream from the netting for different attack angles.These results reveal the existence of turbulent flow due to the netting wake.It was found that the equivalent stress and total deformation increase as the flow velocity increases and solidity ratio decreases. 展开更多
关键词 Knotless netting Fluid structure interaction Deformation characteristics Flow field SST k-omega model
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氨甲环酸对创伤出血性休克肝脏损伤的保护作用及机制
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作者 李志鹏 巫家霖 程新生 《吉林医学》 2025年第5期1039-1042,共4页
目的:探讨氨甲环酸(TXA)对创伤出血性休克(THS)肝脏损伤的保护作用及机制。方法:通过建立THS动物模型,评估TXA在模型中的保护效果。分别检测小鼠血流动力学、肝功能、促炎/抗炎因子、中性粒细胞胞外诱捕网(NETs)水平、自噬相关蛋白变化... 目的:探讨氨甲环酸(TXA)对创伤出血性休克(THS)肝脏损伤的保护作用及机制。方法:通过建立THS动物模型,评估TXA在模型中的保护效果。分别检测小鼠血流动力学、肝功能、促炎/抗炎因子、中性粒细胞胞外诱捕网(NETs)水平、自噬相关蛋白变化。结果:TXA显著改善肝功能,降低丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、NETs和促炎因子[白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α]水平,提高抗炎因子IL-10水平,差异有统计学意义(P<0.05)。此外,TXA还通过抑制Toll样受体(TLR)4通路和自噬相关蛋白LC3的表达减少了炎性反应。结论:TXA在THS模型中能够有效减轻肝脏损伤,其保护机制涉及多条炎性反应和细胞损伤途径。首先,TXA通过抑制TLR4激活,阻断了损伤相关分子模式(DAMPs)引发的炎性反应级联反应,减少促炎细胞因子如IL-1β和TNF-α释放,抑制了由TLR4介导的过度炎性反应。其次,TXA通过调控PI3K/Akt/mTOR信号通路,抑制自噬相关蛋白表达,避免了自噬过程的异常激活,这在减轻细胞损伤和维持细胞内稳态方面发挥了关键作用。此外,TXA还显著抑制了NETs的过度形成,避免了NETs对肝脏组织的直接损伤和其在炎性反应中的放大作用。通过多重机制的共同作用,TXA有效缓解了THS引起的肝脏损伤,保护了肝脏功能。 展开更多
关键词 氨甲环酸 创伤出血性休克 肝脏损伤 NETS TLR4
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敲低MUC5B表达对分泌性中耳炎大鼠炎症反应的影响
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作者 王丹 杨启梅 +2 位作者 刘晓娜 陈菁华 王玉霞 《山西医科大学学报》 2025年第1期57-65,共9页
目的 探究黏蛋白5B(MUC5B)对分泌性中耳炎(SOM)炎症反应的调控作用,以及与中性粒细胞胞外诱捕网(NETs)形成的关系。方法 采用腹腔注射卵清蛋白构建SOM大鼠模型,随机分为模型组、shRNA-NC组和shRNA-MUC5B组,每组10只,另取10只健康SD大鼠... 目的 探究黏蛋白5B(MUC5B)对分泌性中耳炎(SOM)炎症反应的调控作用,以及与中性粒细胞胞外诱捕网(NETs)形成的关系。方法 采用腹腔注射卵清蛋白构建SOM大鼠模型,随机分为模型组、shRNA-NC组和shRNA-MUC5B组,每组10只,另取10只健康SD大鼠作为对照组。末次注射完成后24 h,进行大鼠炎症评分,采用HE染色检测大鼠中耳黏膜组织病变,ELISA法检测中耳灌洗液中炎性因子的表达,流式细胞术检测Th1和Th2细胞分布比例,免疫荧光染色检测中耳黏膜组织中NETs标志物髓过氧化物酶(MPO)和瓜氨酸化组蛋白H3(CitH3)的表达,DCFH-DA探针检测中耳黏膜组织中ROS水平。RTqPCR检测MUC5B基因的表达水平,Western blot检测干扰素-γ(IFN-γ)、白细胞介素-2(IL-2)、IL-4、IL-13、MUC5B、MPO、CitH3和中性粒细胞弹性蛋白酶(NE)蛋白的表达水平。结果 与对照组比较,模型组和shRNA-NC组大鼠表现出中耳黏膜增厚,炎症程度评分升高(P<0.05);中耳黏膜组织中MUC5B mRNA水平,CitH3、MPO、MUC5B、IL-4和IL-13蛋白水平,Th2细胞比例以及ROS水平均升高(P<0.05),IFN-γ和IL-2蛋白水平以及Th1细胞比例和Th1/Th2比值均降低(P<0.05);中耳灌洗液中MPO、NE和CitH3蛋白水平以及IL-4和IL-13含量均升高(P<0.05),IFN-γ和IL-2含量以及IFN-γ/IL-4比值均降低(均P<0.05)。与shRNA-NC组比较,shRNA-MUC5B组大鼠中耳黏膜厚度和炎症程度评分均降低(P<0.05);中耳黏膜组织中MUC5B mRNA水平,CitH3、MPO、MUC5B、IL-4和IL-13蛋白水平,Th2细胞比例以及ROS水平均降低(P<0.05),IFN-γ和IL-2蛋白水平以及Th1细胞比例和Th1/Th2比值均升高(P<0.05);中耳灌洗液中MPO、NE和CitH3蛋白水平以及IL-4和IL-13含量均降低(P<0.05),IFN-γ和IL-2含量以及IFN-γ/IL-4比值均升高(P<0.05)。结论 MUC5B敲低表达可以缓解SOM大鼠中耳黏膜组织炎症反应,该作用可能与抑制NETs的形成有关。 展开更多
关键词 黏蛋白5B(MUC5B) 分泌性中耳炎 炎症反应 中性粒细胞胞外诱捕网(NETs) 中耳黏膜 TH1/TH2
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上海交通大学医学院附属第九人民医院苏文如团队发现中性粒细胞-CD44-Th17循环驱动白塞病葡萄膜炎
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《上海交通大学学报(医学版)》 北大核心 2025年第3期270-270,共1页
2025年2月27日,上海交通大学医学院附属第九人民医院苏文如团队在Advanced Science杂志上发表题为“NETs-CD44-IL-17A feedback loop drives Th17-mediated inflammation in Behçet′s uveitis”的文章。这项研究通过整合临床样本... 2025年2月27日,上海交通大学医学院附属第九人民医院苏文如团队在Advanced Science杂志上发表题为“NETs-CD44-IL-17A feedback loop drives Th17-mediated inflammation in Behçet′s uveitis”的文章。这项研究通过整合临床样本分析、单细胞测序及实验性自身免疫性葡萄膜炎模型,首次揭示了中性粒细胞胞外陷阱(neutrophil extracellular traps,NETs)与Th17细胞间形成的“NETs-CD44-IL-17A正反馈环路”在白塞病葡萄膜炎进展中的核心作用。进一步研究发现,靶向该环路的关键节点(如CD44)可有效阻断炎症级联反应。该成果为白塞病葡萄膜炎的精准治疗提供了新思路。 展开更多
关键词 TH17细胞 CD44 白塞病葡萄膜炎 NETS IL-17A
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人参养荣汤通过调控ROS/MPO减少中性粒细胞胞外诱捕网缓解骨髓抑制
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作者 张靖 刘荣兴 +1 位作者 曾进浩 年青 《中国实验方剂学杂志》 北大核心 2025年第6期39-46,共8页
目的:探讨人参养荣汤调节活性氧(ROS)、髓过氧化物酶(MPO)及中性粒细胞胞外诱捕网(NETs)的表达从而缓解骨髓抑制的潜在机制。方法:将K562细胞分为空白组,依托泊苷组(40μmol·L^(-1)),依托泊苷+人参养荣汤(冻干粉)低、中、高质量浓... 目的:探讨人参养荣汤调节活性氧(ROS)、髓过氧化物酶(MPO)及中性粒细胞胞外诱捕网(NETs)的表达从而缓解骨髓抑制的潜在机制。方法:将K562细胞分为空白组,依托泊苷组(40μmol·L^(-1)),依托泊苷+人参养荣汤(冻干粉)低、中、高质量浓度组(2、4、8 g·L^(-1))。液相色谱-质谱联用技术(LC-MS)检测人参养荣汤冻干粉成分;酶联免疫吸附测定法(ELISA)检测各组ROS、MPO及NETs表达;蛋白免疫印迹法(Western blot)检测胞内MPO和中性粒细胞弹性蛋白酶(NE)表达。将20只8周龄雄性小鼠随机分为正常组,依托泊苷组(100 mg·kg^(-1)),依托泊苷+人参养荣汤低、中、高剂量组(0.1、0.5、2.0 g·kg^(-1)),除空白组常温磷酸盐缓冲液(PBS)灌胃,依托泊苷组腹腔注射3 d外,剩余组依托泊苷给药3 d后人参养荣汤连续灌胃14 d。血常规分析仪检测小鼠外周血象相关指标;Western blot检测小鼠骨髓细胞内MPO及NE表达改变;ELISA检测小鼠骨髓细胞ROS,MPO,NETs改变;小鼠股骨行MPO、NE免疫组化染色;电镜扫描分析小鼠骨髓细胞中NETs结构变化。结果:LC-MS结果显示人参养荣汤冻干粉中含有当归、黄芪、人参等完整的原药成分;K562细胞中,与依托泊苷组比较,ELISA结果表明依托泊苷+人参养荣汤高、中质量浓度组MPO、ROS及NETs水平均降低(P<0.05,P<0.01),同时Western blot显示高质量浓度组明显降低K562细胞内MPO、NE蛋白表达(P<0.05,P<0.01)。小鼠体内,与依托泊苷组比较,依托泊苷+人参养荣汤高质量浓度组红细胞(RBC)、白细胞(WBC)及血小板(PLT)数量上升(P<0.05);ELISA结果提示依托泊苷+人参养荣汤低、中、高质量浓度组小鼠体内ROS、MPO、NETs浓度降低(P<0.05,P<0.01);Western blot结果说明依托泊苷+人参养荣汤低、中、高质量浓度组小鼠骨髓细胞内MPO、NE表达较依托泊苷组降低(P<0.05,P<0.01);扫描电镜观察人参养荣汤可减少依托泊苷作用后小鼠骨髓细胞内NETs结构生成。结论:人参养荣汤通过抑制ROS/MPO,减少细胞内NETs形成,从而缓解依托泊苷诱导的骨髓抑制。 展开更多
关键词 人参养荣汤 髓过氧化物酶(MPO) 活性氧(ROS) 中性粒细胞胞外诱捕网(NETs) 骨髓抑制
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Get Around
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《China Textile》 2025年第5期78-79,共2页
Train Mass Rapid Transit(MRT)was put into service in 1987,and has since been augmented by and linked to the Light Rapid Transit.Combined,you can often get you within walking distance of most destinations.The maps on t... Train Mass Rapid Transit(MRT)was put into service in 1987,and has since been augmented by and linked to the Light Rapid Transit.Combined,you can often get you within walking distance of most destinations.The maps on the metro system are easy to read,complete with English version.You can easily purchase an EZ-Link card or a NETS Flashpay Card(stored value cards)at all MRT stations and bus interchange. 展开更多
关键词 Nets Flashpay Card nets flashpay card stored train mass rapid transit mrt Light Rapid Transit EZ Link Card Stored Value Cards light rapid MRT
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EFI-SATL:An Efficient Net and Self-Attention Based Biometric Recognition for Finger-Vein Using Deep Transfer Learning
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作者 Manjit Singh Sunil Kumar Singla 《Computer Modeling in Engineering & Sciences》 2025年第3期3003-3029,共27页
Deep Learning-based systems for Finger vein recognition have gained rising attention in recent years due to improved efficiency and enhanced security.The performance of existing CNN-based methods is limited by the pun... Deep Learning-based systems for Finger vein recognition have gained rising attention in recent years due to improved efficiency and enhanced security.The performance of existing CNN-based methods is limited by the puny generalization of learned features and deficiency of the finger vein image training data.Considering the concerns of existing methods,in this work,a simplified deep transfer learning-based framework for finger-vein recognition is developed using an EfficientNet model of deep learning with a self-attention mechanism.Data augmentation using various geometrical methods is employed to address the problem of training data shortage required for a deep learning model.The proposed model is tested using K-fold cross-validation on three publicly available datasets:HKPU,FVUSM,and SDUMLA.Also,the developed network is compared with other modern deep nets to check its effectiveness.In addition,a comparison of the proposed method with other existing Finger vein recognition(FVR)methods is also done.The experimental results exhibited superior recognition accuracy of the proposed method compared to other existing methods.In addition,the developed method proves to be more effective and less sophisticated at extracting robust features.The proposed EffAttenNet achieves an accuracy of 98.14%on HKPU,99.03%on FVUSM,and 99.50%on SDUMLA databases. 展开更多
关键词 Biometrics finger-vein recognition(FVR) deep net self-attention Efficient Nets transfer learning
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Netting Talent Deficit From Student Exchange With U.S.
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作者 Liu Yunyun 《Beijing Review》 2019年第16期48-48,共1页
Math is the foundation of everything. The Chinese know this too well and have dominated international math competitions for years. So when the news came that U.S. high school students had won the first prize for a sec... Math is the foundation of everything. The Chinese know this too well and have dominated international math competitions for years. So when the news came that U.S. high school students had won the first prize for a second consecutive year at the Romanian Master of Mathematics 2019, it was no laughing matter. 展开更多
关键词 netting TALENT DEFICIT STUDENT EXCHANGE U.S.
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Technical Regulations for Protected Production of Netted Melon
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作者 Hongwei ZANG Meiguang BI +1 位作者 Lizhi YU Jinhua MOU 《Agricultural Biotechnology》 2025年第5期4-7,共4页
This paper summarized the technical regulations for protected production of netted melon in Yantai City,primarily including technical measures such as the environmental requirements of the production area,cultivation ... This paper summarized the technical regulations for protected production of netted melon in Yantai City,primarily including technical measures such as the environmental requirements of the production area,cultivation techniques,pest and disease control,harvesting,packaging,storage,waste management,and production records for netted melon grown in facilities.The technical regulations aim to standardize the protected production of netted melon and enhance the level of standardized and normative production technology. 展开更多
关键词 FACILITY Netted melon PRODUCTION Technical regulations
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Developmental properties of parvalbumin-positive gamma-aminobutyric acid interneurons and the effect of fluoxetine treatment and binocular form deprivation on them in the visual cortex of adult rats
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作者 Qiao-Yun Wang Tao-Tao Xu +6 位作者 Xing-Hao Wen Man-Hui Zhu Gao-Yu Shen Yi-Qian Xu Yang Guo Lai-Qing Xie Xiao-Yan Ji 《International Journal of Ophthalmology(English edition)》 2025年第12期2237-2245,共9页
AIM:To investigate the postnatal development of parvalbumin(PV)-positive gamma-aminobutyric acid(GABA)interneurons and the co-expression of perineuronal nets(PNNs)and PV in the visual cortex of rats,as well as the reg... AIM:To investigate the postnatal development of parvalbumin(PV)-positive gamma-aminobutyric acid(GABA)interneurons and the co-expression of perineuronal nets(PNNs)and PV in the visual cortex of rats,as well as the regulatory effects of fluoxetine(FLX)treatment and binocular form deprivation(BFD)on these indices.METHODS:Wistar rats were assigned to three experimental cohorts:1)Age-related groups:postnatal week(PW)1,PW3,PW5,PW7,and PW9;2)FLX treatment duration groups:FLX 0W,FLX 2W,FLX 4W,FLX 6W,and FLX 8W;3)Intervention groups:control(Cont),FLX,BFD,and BFD+FLX.The levels of PNNs,PV,and PNNs/PV coexpression in the visual cortex were detected and analyzed.RESULTS:The density of PV-positive cells and the coexpression of PNNs and PV increased gradually with the maturation of the visual cortex(b=0.960,P<0.01).The ratio of PV-positive cells surrounded by PNNs to total PV-positive cells(PNNs+/PV+/total PV+)was significantly decreased in the FLX 4W group(χ^(2)=9.03,P=0.003).There was no significant difference in the PNNs+/PV+/total PV+ratio between the FLX and BFD groups(χ^(2)=1.08,P=0.161),but a significant difference was observed between the BFD+FLX group and the BFD group(χ^(2)=5.82,P<0.01).CONCLUSION:The number of PV-positive neurons and PNNs-surrounded PV neurons in the rat visual cortex increases postnatally and reaches adult levels by postnatal week 7.Chronic FLX treatment downregulates these expressions.Combined 4-week FLX treatment and BFD exerts a more significant inhibitory effect on the PNNs+/PV+/total PV+ratio than either intervention alone. 展开更多
关键词 PARVALBUMIN gamma-aminobutyric acid perineuronal nets FLUOXETINE visual cortex plasticity
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Investigating the Role of Antimalarial Treatment and Mosquito Nets in Malaria Transmission and Control through Mathematical Modeling
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作者 Azhar Iqbal Kashif Butt Tariq Ismaeel +4 位作者 Sara Khan Muhammad Imran Waheed Ahmad Ismail Abdulrashid Muhammad Sajid Riaz 《Computer Modeling in Engineering & Sciences》 2025年第9期3463-3492,共30页
Malaria is a significant global health challenge.This devastating disease continues to affect millions,especially in tropical regions.It is caused by Plasmodium parasites transmitted by female Anopheles mosquitoes.Thi... Malaria is a significant global health challenge.This devastating disease continues to affect millions,especially in tropical regions.It is caused by Plasmodium parasites transmitted by female Anopheles mosquitoes.This study introduces a nonlinear mathematical model for examining the transmission dynamics of malaria,incorporating both human and mosquito populations.We aim to identify the key factors driving the endemic spread of malaria,determine feasible solutions,and provide insights that lead to the development of effective prevention and management strategies.We derive the basic reproductive number employing the next-generation matrix approach and identify the disease-free and endemic equilibrium points.Stability analyses indicate that the disease-free equilibrium is locally and globally stable when the reproductive number is below one,whereas an endemic equilibrium persists when this threshold is exceeded.Sensitivity analysis identifies the most influential mosquito-related parameters,particularly the bite rate and mosquito mortality,in controlling the spread of malaria.Furthermore,we extend our model to include a treatment compartment and three disease-preventive control variables such as antimalaria drug treatments,use of larvicides,and the use of insecticide-treated mosquito nets for optimal control analysis.The results show that optimal use of mosquito nets,use of larvicides for mosquito population control,and treatment can lower the basic reproduction number and control malaria transmission with minimal intervention costs.The analysis of disease control strategies and findings offers valuable information for policymakers in designing cost-effective strategies to combat malaria. 展开更多
关键词 MALARIA mathematical modeling optimal control mosquito nets anti-malaria drugs stability and sensitivity analysis
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