Neuromedin B(NMB)is a member of mammalian bombesin-like peptide family and almost all its potent biological actions take place exclusively through its receptor NMBR,which has been identified as a G-protein couple rece...Neuromedin B(NMB)is a member of mammalian bombesin-like peptide family and almost all its potent biological actions take place exclusively through its receptor NMBR,which has been identified as a G-protein couple receptor with seven trans-membrane regions.NMB and NMBR represent an interesting signaling axis over which NMB/NMBR perform multiple biological activities,in particularly,they have been highlighted in the regulation of the immunology system.This review aims to provide a brief overview of the recent advancements in our understanding regarding the potential immunological functions of NMB and NMBR in cell proliferation,tumorigenesis,angiogenesis,neurogenic inflammation,anti-influenza A virus(IAV)infections,and briefly discusses the potential importance of these observations.All these implicated that targeting NMBR or developing analogues of NMB may represent a useful strategy for development of new anti-IAV agents.展开更多
[Objectives]This study was conducted to investigate the effect of lipopolysaccharide(LPS)on the gene expression levels of neuromedin B(NMB)and its receptor(NMBR)in the hypothalamic-pituitary-testis(HPT)axis of male ra...[Objectives]This study was conducted to investigate the effect of lipopolysaccharide(LPS)on the gene expression levels of neuromedin B(NMB)and its receptor(NMBR)in the hypothalamic-pituitary-testis(HPT)axis of male rabbits.[Methods]Forty male New Zealand white rabbits were randomly divided into an experimental group injected with LPS and a control group.At 0,1.5,3,6 and 12 h after LPS injection,four rabbits in each group were sacrificed,and the hypothalamus,pituitary and testicular tissues were collected.RT-PCR was carried out to detect the expression of rabbit NMB and NMBR mRNA in the HPT axis.[Results]NMB and NMBR mRNA were expressed in the hypothalamus,pituitary gland and testis of rabbits,and there was a certain time effect,indicating that the NMB/NMBR system is involved in the activities of the hypothalamus-pituitary-testis(HPT)axis.At 1.5 h after LPS stimulation,the expression of NMB mRNA in the hypothalamus increased significantly,and showed an extremely significant difference from the control group(P<0.01),while the expression of NMBR mRNA in the hypothalamus increased,but was not significantly different from the control group(P>0.05).The expression levels of NMB and NMBR mRNA in the pituitary and testis tissues in the HPT axis at different time after the LPS treatment were not significantly different from those in the control group(P>0.05).Therefore,LPS stimulation still has a certain effect on the expression of NMB and its receptor mRNA in the HPT axis.[Conclusions]This study is of great significance for the in-depth study on the mechanism of damage caused by immune stress to livestock and poultry reproduction.展开更多
目的构建小鼠神经介素B(neuromedin B,NMB)及其受体(neuromedin B receptor,NMBR)基因慢病毒过表达质粒,并于RAW264.7细胞中表达,以期为小鼠NMB和NMBR基因过表达对RAW264.7细胞增殖及凋亡影响的深入研究奠定基础。方法经RT-PCR法扩增获...目的构建小鼠神经介素B(neuromedin B,NMB)及其受体(neuromedin B receptor,NMBR)基因慢病毒过表达质粒,并于RAW264.7细胞中表达,以期为小鼠NMB和NMBR基因过表达对RAW264.7细胞增殖及凋亡影响的深入研究奠定基础。方法经RT-PCR法扩增获得小鼠NMB和NMBR基因的编码序列(coding sequence,CDS),插入载体pCD513B-1,构建重组质粒pCD513B-1-NMB和pCD513B-1-NMBR;于HEK-293T细胞包装获得小鼠NMB和NMBR基因过表达慢病毒,通过倍比稀释法检测病毒效价。用慢病毒感染RAW264.7细胞48 h后,qPCR法检测小鼠NMB和NMBR基因mRNA的表达情况,以未感染细胞为对照。结果经双酶切鉴定证明重组质粒构建正确。小鼠NMB和NMBR基因过表达慢病毒滴度分别为6×10~6和8×10~6TU/mL。2种慢病毒转染RAW264.7细胞中小鼠NMB和NMBR基因mRNA表达量显著高于对照组(t分别为24.158和14.958,P均<0.01)。结论成功构建了小鼠NMB和NMBR基因过表达质粒,能够显著提高RAW264.7细胞NMB和NMBR基因的表达量。展开更多
基金National Science Foundation of China(U1805231)National Program for Innovation and Entrepreneurship Training of University Students(202110389008)+2 种基金Special Fund Project for Scientific and Technological Innovation of Fujian Agriculture and Forestry University(CXZX2018015,CXZX2020060A)Key Laboratory Project of Animal Infectious Disease Control and Biotechnology in Fujian Province(2018KF03)National Science Foundation of Fujian Province(2020J01539).
文摘Neuromedin B(NMB)is a member of mammalian bombesin-like peptide family and almost all its potent biological actions take place exclusively through its receptor NMBR,which has been identified as a G-protein couple receptor with seven trans-membrane regions.NMB and NMBR represent an interesting signaling axis over which NMB/NMBR perform multiple biological activities,in particularly,they have been highlighted in the regulation of the immunology system.This review aims to provide a brief overview of the recent advancements in our understanding regarding the potential immunological functions of NMB and NMBR in cell proliferation,tumorigenesis,angiogenesis,neurogenic inflammation,anti-influenza A virus(IAV)infections,and briefly discusses the potential importance of these observations.All these implicated that targeting NMBR or developing analogues of NMB may represent a useful strategy for development of new anti-IAV agents.
基金National Natural Science Foundation of China(31802149,31372388)Natural Science Foundation of Jiangsu Province(BK20180919)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘[Objectives]This study was conducted to investigate the effect of lipopolysaccharide(LPS)on the gene expression levels of neuromedin B(NMB)and its receptor(NMBR)in the hypothalamic-pituitary-testis(HPT)axis of male rabbits.[Methods]Forty male New Zealand white rabbits were randomly divided into an experimental group injected with LPS and a control group.At 0,1.5,3,6 and 12 h after LPS injection,four rabbits in each group were sacrificed,and the hypothalamus,pituitary and testicular tissues were collected.RT-PCR was carried out to detect the expression of rabbit NMB and NMBR mRNA in the HPT axis.[Results]NMB and NMBR mRNA were expressed in the hypothalamus,pituitary gland and testis of rabbits,and there was a certain time effect,indicating that the NMB/NMBR system is involved in the activities of the hypothalamus-pituitary-testis(HPT)axis.At 1.5 h after LPS stimulation,the expression of NMB mRNA in the hypothalamus increased significantly,and showed an extremely significant difference from the control group(P<0.01),while the expression of NMBR mRNA in the hypothalamus increased,but was not significantly different from the control group(P>0.05).The expression levels of NMB and NMBR mRNA in the pituitary and testis tissues in the HPT axis at different time after the LPS treatment were not significantly different from those in the control group(P>0.05).Therefore,LPS stimulation still has a certain effect on the expression of NMB and its receptor mRNA in the HPT axis.[Conclusions]This study is of great significance for the in-depth study on the mechanism of damage caused by immune stress to livestock and poultry reproduction.
