The naturally fermented Inner Mongolian cheese’s flavor and nutritional value make it a popular choice among customers.In this work,to create multi-functional peptides that have taste and biological activity,peptidom...The naturally fermented Inner Mongolian cheese’s flavor and nutritional value make it a popular choice among customers.In this work,to create multi-functional peptides that have taste and biological activity,peptidomics and bioinformatics were used to screen flavor peptides from Inner Mongolian cheese and further assess their antioxidant and angiotensin I-converting enzyme(ACE)inhibitory properties.According to sensory data,YH8 and IL7 had detectable bitter tastes with low thresholds of 0.03 and 0.06 mmol/L,respectively.With an umami threshold range of 0.24‒0.81 mmol/L,VQ6,FK13,HP13 and QT14 exhibited a range of flavors dominated by umami,including sweet,bitter,salty,sour and kokumi.Antioxidant activity wise,YH8,VQ6,HP13 and QT14 were well represented.The above-mentioned peptides all had some ACE inhibitory effect.The bitter peptide IL7(IC_(50)=0.08 mmol/L)had the highest level of ACE inhibitory activity,followed by YH8(IC_(50)=0.33 mmol/L).These multi-functional peptides,which have been assessed for bioactive and taste features in Inner Mongolian cheese,may have positive impacts on health and harmonize the cheese’s overall flavor.These results suggest that some flavor peptides produced in fermented foods might be with bioactivities while providing a basis for the exploration and application of multi-functional peptides.展开更多
Figure 6(a)in the paper[Chin.Phys.B 33074203(2024)]was incorrect due to editorial oversight.The correct figure is provided.This modification does not affect the result presented in the paper.
The gut microbiome is closely associated with human health and the development of diseases. Isolating, characterizing, and identifying gut microbes are crucial for research on the gut microbiome and essential for adva...The gut microbiome is closely associated with human health and the development of diseases. Isolating, characterizing, and identifying gut microbes are crucial for research on the gut microbiome and essential for advancing our understanding and utilization of it. Although culture-independent approaches have been developed, a pure culture is required for in-depth analysis of disease mechanisms and the development of biotherapy strategies. Currently, microbiome research faces the challenge of expanding the existing database of culturable gut microbiota and rapidly isolating target microorganisms. This review examines the advancements in gut microbe isolation and cultivation techniques, such as culturomics, droplet microfluidics, phenotypic and genomics selection, and membrane diffusion. Furthermore, we evaluate the progress made in technology for identifying gut microbes considering both non-targeted and targeted strategies. The focus of future research in gut microbial culturomics is expected to be on high-throughput, automation, and integration. Advancements in this field may facilitate strain-level investigation into the mechanisms underlying diseases related to gut microbiota.展开更多
OBJECTIVE:To investigate the effects of gut microbes regulation of the trimethylamine(TMA)/flavin containing monooxygenase 3(FMO3)/trimethylamine N-oxide(TMAO)pathway on platelet aggregation in acute coronary syndrome...OBJECTIVE:To investigate the effects of gut microbes regulation of the trimethylamine(TMA)/flavin containing monooxygenase 3(FMO3)/trimethylamine N-oxide(TMAO)pathway on platelet aggregation in acute coronary syndrome(ACS)rats and the intervention of Huayu Qutan formula(化瘀祛痰方).METHODS:The ACS rats with syndrome of phlegm and blood stasis rats were established.Platelet,platelet aggregation,platelet activation markers and TMA/FMO3/TMAO pathway were detected.Metagenomics technology was employed to analyze the characteristics of the gut microbiota.RESULTS:Huayu Qutan formula and gut microbes could inhibit high platelet reactivity and regulate the TMA/FMO3/TMAO pathway.The dominant bacteria in ACS rats including but not limited to the major phyla,Firmicutes,Bacteroidetes,Actinobacteria,and Proteobacteria,also including some low abundance phyla,Fusobacteria,Verrucomicrobia,Spirochaetes,and Deferribacteres.The dominant bacteria in the Huayu Qutan formula group were Synergistetes,Deferribacteres,Deferribacteraceae,Faecalibacterium and Mucispirillum.In the Huayu Qutan formula combined with fecal bacteria enema group,the dominant bacteria were Verrucomicrobia,Verrucomicrobiae,Akkermansia and Verrucomicrobium.These gut microbiota were correlated with pathways such as Riboflavin metabolism and Arachidonic acid metabolism.CONCLUSION:Huayu Qutan formula may prevent ACS by modulating gut microbes Synergistetes,Faecalibacterium and Allobaculum,regulating the iron metabolism of Deferribacteres,and driving the TMA/FMO3/TMAO pathway to regulate gut microbiota function,and improving platelet aggregation.Akkermansia may serve as a promising probiotic,which could drive TMA/FMO3/TMAO pathway to regulate Arachidonic acid metabolism to improve platelet aggregation.The findings of this study provide a theoretical basis for the theory of"the heart is connected with the small intestine".展开更多
As industrial pollution continues to contaminate ecosystems worldwide,researchers have engineered a breakthrough solution:a single bacterial strain that can tackle five persistent organic pollutants at once.
This study establishes and validates a method for the precise quantification of aquatic microbial loads using microbial diversity absolute quantitative sequencing.By adding synthetic spike-in DNA to water samples from...This study establishes and validates a method for the precise quantification of aquatic microbial loads using microbial diversity absolute quantitative sequencing.By adding synthetic spike-in DNA to water samples from the Dahei River prior to DNA extraction and 16S rRNA gene sequencing,it generates standard curves to convert sequencing data into absolute microbial copy numbers.The method,which is proved highly accurate(R^(2)>0.99),reveals a clear contrast between the river sites:the upstream community has not only a significantly higher total microbial load but also a completely different makeup of species compared to the downstream site.This approach effectively overcomes the limitations of relative abundance analysis,providing a powerful tool for environmental monitoring,and proposes key steps for future standardization to ensure data comparability and integration.展开更多
Three typical rural-suburban-urban artificial wetlands Pinus elliottii forest in Nanchang City were selected as research objects to mensurate the soil nutrient content.And the annual average values and seasonal change...Three typical rural-suburban-urban artificial wetlands Pinus elliottii forest in Nanchang City were selected as research objects to mensurate the soil nutrient content.And the annual average values and seasonal changes of microbes in forest soil were analyzed.The results showed that soil bulk density,total phosphorus （TP） and pH increased,while soil organic carbon （SOC）,total nitrogen （TN） declined with rural-to-urban gradient.At different eco-boundary,annual average values showed that actinomycetes quantity bacteria quantity fungi quantity.Total microbe number was urban suburb rural areas.The number of bacteria and fungi was urban suburbs rural areas,but the number of actinomycetes was suburb urban rural areas.Eco-boundary,season and microbes actinomycetes and fungi reached an extreme significant level （P 0.001）.Bacteria in soil at different eco-boundary had significant effects,but season had no significant effect on bacteria.Eco-boundary and season had a very significant interaction on actinomycetes and fungi （P 0.001）,but they had no significant interaction on bacteria （P 0.05）.Eco-boundary showed significantly positive correlation with actinomycetes and fungi （P 0.001）,but season had no significant corelation with microbes.In conclusion,urbanization process caused the physical-chemical properties changes of forest soil and affected the amount of soil microbes obviously.展开更多
[Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums. [Method] under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determ...[Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums. [Method] under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determined the CMCase and Fpase,then proceeded the 16S rRNA gene analysis. [Result]The results showed that:BC1 and BC3 strains characterized higher carboxymethyl cellulose enzyme activity and higher filter paper activity,but their difference was small,then the 16S rDNA sequence of BC1 and BC3 strains were related to pseudomonas sp. (98% and 99% similarities,respectively).[Conclusion] the experiment laid foundation for high-efficiency biological heating agent.展开更多
Multi-functional Al-matrix composites with high volume fraction （55%-57%） of SiC particles are produced with the new pressureless infiltration fabrication technology. X-ray detection and microscopic observation disp...Multi-functional Al-matrix composites with high volume fraction （55%-57%） of SiC particles are produced with the new pressureless infiltration fabrication technology. X-ray detection and microscopic observation display the composites which are macroscopically homogeneous without porosity. The investigation further reveals that the SiC/Al composites possess low density （2.94 g/cm^3）, high elastic modulus （220 GPa）, prominent thermal management function as a result of low coefficient of thermal expansion （8 × 10^4 K^-1） and high thermal conductivity （235 W/（m.K）） as well as unique preventability of resonance vibration. By adopting a series of developed techniques, the multi-functional SiC/Al composites have managed to be made into near-net-shape parts. Many kinds of precision components of space-based optomechanical structures and airborne optoelectronic platform have been turned out. Of them, several typical products are being under test in practices.展开更多
It has been estimated that a typical gram of soilcontains around 90 100 million bacteria and about200 000 fungi, with the majority of these organismsbeing located around the roots of plants. This locali-zation reflect...It has been estimated that a typical gram of soilcontains around 90 100 million bacteria and about200 000 fungi, with the majority of these organismsbeing located around the roots of plants. This locali-zation reflects the fact that plant roots typically exudea large fraction of the carbon that they fix by photo-synthesis, and soil microbes utilize this exuded carbonas a food source.展开更多
The microbial consortium GF-20(GF-20) can efficiently decompose corn stover at low temperatures. The present study explored the key microbes of GF-20 and evaluated different culture conditions on its composition stabi...The microbial consortium GF-20(GF-20) can efficiently decompose corn stover at low temperatures. The present study explored the key microbes of GF-20 and evaluated different culture conditions on its composition stability to promote the utilization of corn stover decomposing microbes in low temperature regions. GF-20 was subcultured to the 15 th generation under different temperatures, pHs, carbon, and nitrogen sources. Then, the dynamics of fermenting pH, cellulose enzyme activities, carbohydrate concentration, and oxidation reduction potential were determined to estimate the degradation efficiency of corn stover with GF-20. Furthermore, the structural stability and functional microbes of GF-20 were identified on the basis of PCR-denaturing gradient gel electrophoresis(DGGE) profiling and principal component analysis. The results showed that the offspring of GF-20 subcultured under different temperatures(4–30°C) and pH(6.0–9.0) conditions maintained stable growth, decomposition function, and composition structure. Furthermore, consortia GF-20 had a stable composition structure, which induced GF-20 to secrete cellulose and promote substrate decomposition as corn stover and ammonium were used as sources of carbon and nitrogen, respectively. According to the PCR-DGGE profiles, the key strains of GF-20 were determined to be Bacillus licheniformis, Cellvibrio mixtus subsp. mixtus, Bacillus tequilensis, Clostridium populeti, and Clostridium xylanolyticum.展开更多
The thermochemical kinetics of the rare earth ternary complex Sm(Gly) 4Im(ClO 4) 3·2H 2O (Gly glycine, Im imidazole) reacting with microbes (escherichia coli, bacillus subilis, staphylococcus aureus) was st...The thermochemical kinetics of the rare earth ternary complex Sm(Gly) 4Im(ClO 4) 3·2H 2O (Gly glycine, Im imidazole) reacting with microbes (escherichia coli, bacillus subilis, staphylococcus aureus) was studied by means of Calvet microcalorimeter. The rate constant k of microbe growth was calculated, and the inhibition effect of these complexes to microbes was compared.展开更多
In an era of electronics,recovering the precious metal such as gold from ever increasing piles of electronic-wastes and metal-ion infested soil has become one of the prime concerns for researchers worldwide.Biological...In an era of electronics,recovering the precious metal such as gold from ever increasing piles of electronic-wastes and metal-ion infested soil has become one of the prime concerns for researchers worldwide.Biological mining is an attractive,economical and nonhazardous to recover gold from the low-grade auriferous ore containing waste or soil.This review represents the recent major biological gold retrieval methods used to bio-mine gold.The biomining methods discussed in this review include,bioleaching,bio-oxidation,bio-precipitation,bio-flotation,bio-flocculation,bio-sorption,bio-reduction,bioelectrometallurgical technologies and bio accumulation.The mechanism of gold biorecovery by microbes is explained in detail to explore its intracellular mechanistic,which help it withstand high concentrations of gold without causing any fatal consequences.Major challenges and future opportunities associated with each method and how they will dictate the fate of gold bio-metallurgy from metal wastes or metal infested soil bioremediation in the coming future are also discussed.With the help of concurrent advancements in high-throughput technologies,the gold bio-exploratory methods will speed up our ways to ensure maximum gold retrieval out of such low-grade ores containing sources,while keeping the gold mining clean and more sustainable.展开更多
Microbe cementitious material as a binder has been developed due to the ever increasing awareness of environmental protection.The new cementitious material relies on microbiologically induced precipitation of calcium ...Microbe cementitious material as a binder has been developed due to the ever increasing awareness of environmental protection.The new cementitious material relies on microbiologically induced precipitation of calcium carbonate to bind loose particles or repair surface defects and cracks of cement-based material.This paper elaborates the research on loose sand particles cemented by microbe cement from three aspects:compressive strength,pore structure and microstructure.In addition,the research on restoration surface defects and cracks of cement-based material by microbe cement is introduced from two parameters:surface water absorption and compressive strength recover coefficient.The results show that microbe cementitious material can bind loose particles and repair surface defects or cracks of cement-based material.展开更多
The bio-sandstone, which was cemented by microbe cement, was firstly prepared, and then the microstructure evolution process was studied by X-ray computed tomography （X-CT） technique. The experimental results indica...The bio-sandstone, which was cemented by microbe cement, was firstly prepared, and then the microstructure evolution process was studied by X-ray computed tomography （X-CT） technique. The experimental results indicate that the microstructure of bio-sandstone becomes dense with the development of age. The evolution of inner structure at different positions is different due to the different contents of microbial induced precipitation calcite. Besides, the increase rate of microbial induced precipitation calcite gradually decreases because of the reduction of microbe absorption content with the decreasing pore size in bio-sandstone.展开更多
Some strains and culture of bacteria which are able to decolorize dyes and degrade polyvinyl alcohol(PVA) were isolated and selected. A pilot scale facultative anaerobic-aerobic biological process was applied for trea...Some strains and culture of bacteria which are able to decolorize dyes and degrade polyvinyl alcohol(PVA) were isolated and selected. A pilot scale facultative anaerobic-aerobic biological process was applied for treatment of textile wastewater containing dyes and PVA. Activated carbon adsorption was used as a tertiary treatment stage, and residual sludge from clarifier returned to the anaerobic reactor again. The pilot test were carried out with two systems. One was inoculated by acclimated sludge, and the another was adding the mixed culture of dye-decoloring and PVA-degrading bacteria for forming biological films, the latter was observed to be more effective than the former. The test has run normally for ten months with a COD loading of 2.13 kg/m3/day, a BOD5 loading of 0.34 kg/m3/day in anaerobic reactor; a COD loading of 1.71 kg/m3/day, a BOD5 loading 0.44 kg/m3/day in aerobic reactor. The pollutants removal efficiency by adding microbes was about 20% higher than that by acclimated sludge. The average removal efficiency of COD stood about 92%, BOD5 97%, PVA 90% and decolorization 80%. The other parameters of effluent quality are also satisfactory.展开更多
[Objective] The aim was to study on effect of different temperatures on microbes and its quality of green pepper during circulation. [Method] Measurement was conducted on change of colibacillus, salmonella, Listeria n...[Objective] The aim was to study on effect of different temperatures on microbes and its quality of green pepper during circulation. [Method] Measurement was conducted on change of colibacillus, salmonella, Listeria number, and its quality of green pepper stored at 9 and 20 ℃ respectively. The green peppers stored at 9 ℃ for 16 d were under simulated sale. In addition, effects of microbe number of green peppers stored at 20 ℃ for 4 d by different washing methods were measured. [Result] Indices of green pepper were different when green peppers were stored for 16 d at 9 ℃ and sold at 20 ℃ after 16 d storage. Specifically, under the former condition, sensory quality, weight-loss rate, Vc content, chlorophyll content and soluble protein were 4.1, 1.79%, 95.6, 20.3, and 93.3 mg/100 g; under the latter condition, the corresponding indices were 3.3, 3.87%, 81.2, 16.5, 85.6 mg/100 g, respectively. Colibacillus numbers for green pepper stored 16 d at 9 ℃, sold for 1 d at 20 ℃ after 16 d storage and stored for 4 d at 20 ℃ were 46, 3.2×103 and 3.1×104 cfu/g; during the test, salmonella and Listeria were not found; colibacillus in green pepper could be eliminated by 1×10-3 ml/L of chloros and most colibacillus in green pepper could be cleared away by 1 drop/L of detergent and running water. [Conclusion] Stored at 9 ℃, quality of green pepper could be better preserved and colibacillus propagation could be controlled.展开更多
Production and storage-transportation of crude oil can not only give rise to soil pollution but also destroy ecological environment. Degradation of microbes for oily soil was studied with the instnunent, Geofina Hydro...Production and storage-transportation of crude oil can not only give rise to soil pollution but also destroy ecological environment. Degradation of microbes for oily soil was studied with the instnunent, Geofina Hydrocarbon Meter (GHM), by experimental analysis qualitatively and quantitatively in the paper. Analytical result showed that the crude oil could be considerably degraded by eating-oil microbes in oily soil and the number of eating-oil microbes increased while the working hours of oil-well risi...展开更多
Metal-organic frameworks（MOFs） are a unique class of porous crystalline materials that have shown promise for a wide range of applications. MOFs have been explored as a new type of heterogeneous catalytic materials,...Metal-organic frameworks（MOFs） are a unique class of porous crystalline materials that have shown promise for a wide range of applications. MOFs have been explored as a new type of heterogeneous catalytic materials, because of their high surface area, uniform and tunable pores, facile functionalization and incorporation of catalytic active sites. The use of multi-functional sites MOF materials as catalysts for synergistic catalysis and tandem reactions has attracted increasing attention. In this review, we aim to introduce the construction of bi-or multi-functional MOF catalysts with cooperative or cascade functions via post-synthetic modification（PSM）.展开更多
Microbes were cultured from the samples at various depths in a weathering profile of RE-bearing granite in Gonghe RE mine, Guangdong Province. The cultured microbes, existing at a depth of 0.2 similar to 12 in and bei...Microbes were cultured from the samples at various depths in a weathering profile of RE-bearing granite in Gonghe RE mine, Guangdong Province. The cultured microbes, existing at a depth of 0.2 similar to 12 in and being more plentiful within 3 in in the profile, include bacteria ( Bacillus, Enterobacter, Escherichia, Alkaligenes, Neisseria, Staphylococcus and anaerobic bacteria such as Clostridium), fungi ( Aspergillus niger, Aspergillus flavus, Penicillium, Mucor and Saccharomycete) and actinomyces. Experiments were made under room temperature by using solutions of the cultured microbes and their metabolites, compared with distilled water and the culture solution without microbes, to leach RE from the sample of the weathering crust. The results are shown by the experiments: (1) The mixed microbes cultured from the profile and their metabolites increase the quantity of RE leached from the sample and reduce the pH of the solutions. (2) The ability to leach RE from the sample varies with various microbes, decreasing in a sequence of fungi ( Mucor, saccharomycete, Aspegillus and Penicillium), zymotic bacilli (Enterobacter, Escherichia etc.), Staphylococcus, zymotic Bacillus, actinomyces and Alkaligenes. (3) The RE leached with bacteria is mainly related to the pH value of the solutions influenced by the metabolites of the bacteria; whereas that leached with fungi is mainly related to the adsorption and imbibition of RE by the fungi and the complexing of RE with their metabolites. (4) Compared with that leached with ammonium sulfate, the fractionation of the RE leached with microbes is characterized by higher delta (Ce), lower delta (Eu) and lower ratios of N-La/Sm and N-Gd/Yb . The result of the fractionation of RE accords with the distribution of RE in the various layers of a profile of weathering crust of granite in South China. The experimental results indicate that microbes and their metabolites should play a positive role in the mobilization, migration and fractionation of RE in the weathering crust in South China.展开更多
基金supported by the central government and guides local funds for science and technology development(2022ZY0109).
文摘The naturally fermented Inner Mongolian cheese’s flavor and nutritional value make it a popular choice among customers.In this work,to create multi-functional peptides that have taste and biological activity,peptidomics and bioinformatics were used to screen flavor peptides from Inner Mongolian cheese and further assess their antioxidant and angiotensin I-converting enzyme(ACE)inhibitory properties.According to sensory data,YH8 and IL7 had detectable bitter tastes with low thresholds of 0.03 and 0.06 mmol/L,respectively.With an umami threshold range of 0.24‒0.81 mmol/L,VQ6,FK13,HP13 and QT14 exhibited a range of flavors dominated by umami,including sweet,bitter,salty,sour and kokumi.Antioxidant activity wise,YH8,VQ6,HP13 and QT14 were well represented.The above-mentioned peptides all had some ACE inhibitory effect.The bitter peptide IL7(IC_(50)=0.08 mmol/L)had the highest level of ACE inhibitory activity,followed by YH8(IC_(50)=0.33 mmol/L).These multi-functional peptides,which have been assessed for bioactive and taste features in Inner Mongolian cheese,may have positive impacts on health and harmonize the cheese’s overall flavor.These results suggest that some flavor peptides produced in fermented foods might be with bioactivities while providing a basis for the exploration and application of multi-functional peptides.
文摘Figure 6(a)in the paper[Chin.Phys.B 33074203(2024)]was incorrect due to editorial oversight.The correct figure is provided.This modification does not affect the result presented in the paper.
文摘The gut microbiome is closely associated with human health and the development of diseases. Isolating, characterizing, and identifying gut microbes are crucial for research on the gut microbiome and essential for advancing our understanding and utilization of it. Although culture-independent approaches have been developed, a pure culture is required for in-depth analysis of disease mechanisms and the development of biotherapy strategies. Currently, microbiome research faces the challenge of expanding the existing database of culturable gut microbiota and rapidly isolating target microorganisms. This review examines the advancements in gut microbe isolation and cultivation techniques, such as culturomics, droplet microfluidics, phenotypic and genomics selection, and membrane diffusion. Furthermore, we evaluate the progress made in technology for identifying gut microbes considering both non-targeted and targeted strategies. The focus of future research in gut microbial culturomics is expected to be on high-throughput, automation, and integration. Advancements in this field may facilitate strain-level investigation into the mechanisms underlying diseases related to gut microbiota.
基金Supported by National Natural Science Foundation of China Project:based on the Theory of“the Heart is in Harmony with the Small Intestine”to Explore the Influence and Mechanism of Gut Microbes on High Platelet Reactivity of Acute Coronary Syndrome with Phlegm and Blood Stasis Syndrome(No.82104841)Education Department of Liaoning Province Young Science and Technology Talents"Seedling"Project:to Explore the Effect and Mechanism of Huayu Qutan Formula on Platelet Function in Acute Coronary Syndrome Patients with Phlegm and Blood Stasis Syndrome after Percutaneous Coronary Intervention based on Intestinal Microbiome(No.L202039)。
文摘OBJECTIVE:To investigate the effects of gut microbes regulation of the trimethylamine(TMA)/flavin containing monooxygenase 3(FMO3)/trimethylamine N-oxide(TMAO)pathway on platelet aggregation in acute coronary syndrome(ACS)rats and the intervention of Huayu Qutan formula(化瘀祛痰方).METHODS:The ACS rats with syndrome of phlegm and blood stasis rats were established.Platelet,platelet aggregation,platelet activation markers and TMA/FMO3/TMAO pathway were detected.Metagenomics technology was employed to analyze the characteristics of the gut microbiota.RESULTS:Huayu Qutan formula and gut microbes could inhibit high platelet reactivity and regulate the TMA/FMO3/TMAO pathway.The dominant bacteria in ACS rats including but not limited to the major phyla,Firmicutes,Bacteroidetes,Actinobacteria,and Proteobacteria,also including some low abundance phyla,Fusobacteria,Verrucomicrobia,Spirochaetes,and Deferribacteres.The dominant bacteria in the Huayu Qutan formula group were Synergistetes,Deferribacteres,Deferribacteraceae,Faecalibacterium and Mucispirillum.In the Huayu Qutan formula combined with fecal bacteria enema group,the dominant bacteria were Verrucomicrobia,Verrucomicrobiae,Akkermansia and Verrucomicrobium.These gut microbiota were correlated with pathways such as Riboflavin metabolism and Arachidonic acid metabolism.CONCLUSION:Huayu Qutan formula may prevent ACS by modulating gut microbes Synergistetes,Faecalibacterium and Allobaculum,regulating the iron metabolism of Deferribacteres,and driving the TMA/FMO3/TMAO pathway to regulate gut microbiota function,and improving platelet aggregation.Akkermansia may serve as a promising probiotic,which could drive TMA/FMO3/TMAO pathway to regulate Arachidonic acid metabolism to improve platelet aggregation.The findings of this study provide a theoretical basis for the theory of"the heart is connected with the small intestine".
文摘As industrial pollution continues to contaminate ecosystems worldwide,researchers have engineered a breakthrough solution:a single bacterial strain that can tackle five persistent organic pollutants at once.
基金supported by the National Natural Science Foundation of China(Grant No.32160172)the Key Science-Technology Project of Inner Mongolia(2023KYPT0010)+1 种基金the Natural Science Foundation of Inner Mongolia Autonomous Region of China(Grant No.2025QN03006)the 2023 Inner Mongolia Public Institution High-level Talent Introduction Scientific Research Support Project.
文摘This study establishes and validates a method for the precise quantification of aquatic microbial loads using microbial diversity absolute quantitative sequencing.By adding synthetic spike-in DNA to water samples from the Dahei River prior to DNA extraction and 16S rRNA gene sequencing,it generates standard curves to convert sequencing data into absolute microbial copy numbers.The method,which is proved highly accurate(R^(2)>0.99),reveals a clear contrast between the river sites:the upstream community has not only a significantly higher total microbial load but also a completely different makeup of species compared to the downstream site.This approach effectively overcomes the limitations of relative abundance analysis,providing a powerful tool for environmental monitoring,and proposes key steps for future standardization to ensure data comparability and integration.
基金Supported by Natural Science Foundation of Jiangxi Province(2007GQN1935)~~
文摘Three typical rural-suburban-urban artificial wetlands Pinus elliottii forest in Nanchang City were selected as research objects to mensurate the soil nutrient content.And the annual average values and seasonal changes of microbes in forest soil were analyzed.The results showed that soil bulk density,total phosphorus （TP） and pH increased,while soil organic carbon （SOC）,total nitrogen （TN） declined with rural-to-urban gradient.At different eco-boundary,annual average values showed that actinomycetes quantity bacteria quantity fungi quantity.Total microbe number was urban suburb rural areas.The number of bacteria and fungi was urban suburbs rural areas,but the number of actinomycetes was suburb urban rural areas.Eco-boundary,season and microbes actinomycetes and fungi reached an extreme significant level （P 0.001）.Bacteria in soil at different eco-boundary had significant effects,but season had no significant effect on bacteria.Eco-boundary and season had a very significant interaction on actinomycetes and fungi （P 0.001）,but they had no significant interaction on bacteria （P 0.05）.Eco-boundary showed significantly positive correlation with actinomycetes and fungi （P 0.001）,but season had no significant corelation with microbes.In conclusion,urbanization process caused the physical-chemical properties changes of forest soil and affected the amount of soil microbes obviously.
基金Supported by Foundation of Henan Educational Committee(2010B530001)~~
文摘[Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums. [Method] under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determined the CMCase and Fpase,then proceeded the 16S rRNA gene analysis. [Result]The results showed that:BC1 and BC3 strains characterized higher carboxymethyl cellulose enzyme activity and higher filter paper activity,but their difference was small,then the 16S rDNA sequence of BC1 and BC3 strains were related to pseudomonas sp. (98% and 99% similarities,respectively).[Conclusion] the experiment laid foundation for high-efficiency biological heating agent.
基金Foundation items： High-technology Research and Development Programme of China （2007AA03Z544） Aeronautical Science Foundation of China （20075221001）
文摘Multi-functional Al-matrix composites with high volume fraction （55%-57%） of SiC particles are produced with the new pressureless infiltration fabrication technology. X-ray detection and microscopic observation display the composites which are macroscopically homogeneous without porosity. The investigation further reveals that the SiC/Al composites possess low density （2.94 g/cm^3）, high elastic modulus （220 GPa）, prominent thermal management function as a result of low coefficient of thermal expansion （8 × 10^4 K^-1） and high thermal conductivity （235 W/（m.K）） as well as unique preventability of resonance vibration. By adopting a series of developed techniques, the multi-functional SiC/Al composites have managed to be made into near-net-shape parts. Many kinds of precision components of space-based optomechanical structures and airborne optoelectronic platform have been turned out. Of them, several typical products are being under test in practices.
文摘It has been estimated that a typical gram of soilcontains around 90 100 million bacteria and about200 000 fungi, with the majority of these organismsbeing located around the roots of plants. This locali-zation reflects the fact that plant roots typically exudea large fraction of the carbon that they fix by photo-synthesis, and soil microbes utilize this exuded carbonas a food source.
基金supported by the National Natural Science Foundation of China (31760353 and 31560360)the National Key R&D Program of China (2017YFD0300804 and 2016YFD0300103)+2 种基金the earmarked fund for China Agriculture Research System (CARS-02-63)the Crop Science Observation & Experiment Station in Loess Plateau of North China, Ministry of Agriculture, China (25204120)the Advanced Talented Scholars of Inner Mongolia Agricultural University, China (NDYB2016-15)
文摘The microbial consortium GF-20(GF-20) can efficiently decompose corn stover at low temperatures. The present study explored the key microbes of GF-20 and evaluated different culture conditions on its composition stability to promote the utilization of corn stover decomposing microbes in low temperature regions. GF-20 was subcultured to the 15 th generation under different temperatures, pHs, carbon, and nitrogen sources. Then, the dynamics of fermenting pH, cellulose enzyme activities, carbohydrate concentration, and oxidation reduction potential were determined to estimate the degradation efficiency of corn stover with GF-20. Furthermore, the structural stability and functional microbes of GF-20 were identified on the basis of PCR-denaturing gradient gel electrophoresis(DGGE) profiling and principal component analysis. The results showed that the offspring of GF-20 subcultured under different temperatures(4–30°C) and pH(6.0–9.0) conditions maintained stable growth, decomposition function, and composition structure. Furthermore, consortia GF-20 had a stable composition structure, which induced GF-20 to secrete cellulose and promote substrate decomposition as corn stover and ammonium were used as sources of carbon and nitrogen, respectively. According to the PCR-DGGE profiles, the key strains of GF-20 were determined to be Bacillus licheniformis, Cellvibrio mixtus subsp. mixtus, Bacillus tequilensis, Clostridium populeti, and Clostridium xylanolyticum.
文摘The thermochemical kinetics of the rare earth ternary complex Sm(Gly) 4Im(ClO 4) 3·2H 2O (Gly glycine, Im imidazole) reacting with microbes (escherichia coli, bacillus subilis, staphylococcus aureus) was studied by means of Calvet microcalorimeter. The rate constant k of microbe growth was calculated, and the inhibition effect of these complexes to microbes was compared.
基金supported by the University Malaysia Pahang, Malaysiafinancial support provided by university under Research Grant No.RDU190332Ministry of Education of Malaysia under the research Grant of RDU 190121.
文摘In an era of electronics,recovering the precious metal such as gold from ever increasing piles of electronic-wastes and metal-ion infested soil has become one of the prime concerns for researchers worldwide.Biological mining is an attractive,economical and nonhazardous to recover gold from the low-grade auriferous ore containing waste or soil.This review represents the recent major biological gold retrieval methods used to bio-mine gold.The biomining methods discussed in this review include,bioleaching,bio-oxidation,bio-precipitation,bio-flotation,bio-flocculation,bio-sorption,bio-reduction,bioelectrometallurgical technologies and bio accumulation.The mechanism of gold biorecovery by microbes is explained in detail to explore its intracellular mechanistic,which help it withstand high concentrations of gold without causing any fatal consequences.Major challenges and future opportunities associated with each method and how they will dictate the fate of gold bio-metallurgy from metal wastes or metal infested soil bioremediation in the coming future are also discussed.With the help of concurrent advancements in high-throughput technologies,the gold bio-exploratory methods will speed up our ways to ensure maximum gold retrieval out of such low-grade ores containing sources,while keeping the gold mining clean and more sustainable.
基金the National Natural Science Foundation of China(No.51072035)the Ph.D.Program's Foundation of Ministry of Education of China (No.20090092110029)+1 种基金the Research Innovation Program for College Graduates of Jiangsu Province (No.CXZZ 0145)the Scientific Research Foundation of Graduate School of Southeast University (No.YBJJ1127)
文摘Microbe cementitious material as a binder has been developed due to the ever increasing awareness of environmental protection.The new cementitious material relies on microbiologically induced precipitation of calcium carbonate to bind loose particles or repair surface defects and cracks of cement-based material.This paper elaborates the research on loose sand particles cemented by microbe cement from three aspects:compressive strength,pore structure and microstructure.In addition,the research on restoration surface defects and cracks of cement-based material by microbe cement is introduced from two parameters:surface water absorption and compressive strength recover coefficient.The results show that microbe cementitious material can bind loose particles and repair surface defects or cracks of cement-based material.
基金Funded by the National Natural Science Foundation of China(No.51072035),the Ph D Program’s Foundation of Ministry of Education of China(No.20090092110029)the Research Innovation Program for College Graduates of Jiangsu Province(No.CXZZ_0145)the Scientific Research Foundation of Graduate School of Southeast University(Nos.YBJJ1127 and YBPY1208)
文摘The bio-sandstone, which was cemented by microbe cement, was firstly prepared, and then the microstructure evolution process was studied by X-ray computed tomography （X-CT） technique. The experimental results indicate that the microstructure of bio-sandstone becomes dense with the development of age. The evolution of inner structure at different positions is different due to the different contents of microbial induced precipitation calcite. Besides, the increase rate of microbial induced precipitation calcite gradually decreases because of the reduction of microbe absorption content with the decreasing pore size in bio-sandstone.
文摘Some strains and culture of bacteria which are able to decolorize dyes and degrade polyvinyl alcohol(PVA) were isolated and selected. A pilot scale facultative anaerobic-aerobic biological process was applied for treatment of textile wastewater containing dyes and PVA. Activated carbon adsorption was used as a tertiary treatment stage, and residual sludge from clarifier returned to the anaerobic reactor again. The pilot test were carried out with two systems. One was inoculated by acclimated sludge, and the another was adding the mixed culture of dye-decoloring and PVA-degrading bacteria for forming biological films, the latter was observed to be more effective than the former. The test has run normally for ten months with a COD loading of 2.13 kg/m3/day, a BOD5 loading of 0.34 kg/m3/day in anaerobic reactor; a COD loading of 1.71 kg/m3/day, a BOD5 loading 0.44 kg/m3/day in aerobic reactor. The pollutants removal efficiency by adding microbes was about 20% higher than that by acclimated sludge. The average removal efficiency of COD stood about 92%, BOD5 97%, PVA 90% and decolorization 80%. The other parameters of effluent quality are also satisfactory.
基金Supported by National Science&Technology Pillar Program during the Eleventh Five-year Plan Period(2009BADB91309-6)Construction of Massive Vegetable Industry(CARS-25-E-01)Special Fund of Beijing Academy of Agriculture and Forestry for Innovation of Sciences and Technology(KJCX201102004)~~
文摘[Objective] The aim was to study on effect of different temperatures on microbes and its quality of green pepper during circulation. [Method] Measurement was conducted on change of colibacillus, salmonella, Listeria number, and its quality of green pepper stored at 9 and 20 ℃ respectively. The green peppers stored at 9 ℃ for 16 d were under simulated sale. In addition, effects of microbe number of green peppers stored at 20 ℃ for 4 d by different washing methods were measured. [Result] Indices of green pepper were different when green peppers were stored for 16 d at 9 ℃ and sold at 20 ℃ after 16 d storage. Specifically, under the former condition, sensory quality, weight-loss rate, Vc content, chlorophyll content and soluble protein were 4.1, 1.79%, 95.6, 20.3, and 93.3 mg/100 g; under the latter condition, the corresponding indices were 3.3, 3.87%, 81.2, 16.5, 85.6 mg/100 g, respectively. Colibacillus numbers for green pepper stored 16 d at 9 ℃, sold for 1 d at 20 ℃ after 16 d storage and stored for 4 d at 20 ℃ were 46, 3.2×103 and 3.1×104 cfu/g; during the test, salmonella and Listeria were not found; colibacillus in green pepper could be eliminated by 1×10-3 ml/L of chloros and most colibacillus in green pepper could be cleared away by 1 drop/L of detergent and running water. [Conclusion] Stored at 9 ℃, quality of green pepper could be better preserved and colibacillus propagation could be controlled.
基金Supported by the nature scientific fund of Heilongjiang province(No.110210).
文摘Production and storage-transportation of crude oil can not only give rise to soil pollution but also destroy ecological environment. Degradation of microbes for oily soil was studied with the instnunent, Geofina Hydrocarbon Meter (GHM), by experimental analysis qualitatively and quantitatively in the paper. Analytical result showed that the crude oil could be considerably degraded by eating-oil microbes in oily soil and the number of eating-oil microbes increased while the working hours of oil-well risi...
基金supported by the National Natural Science Foundation of China (Nos. 21371069 and 21621001)
文摘Metal-organic frameworks（MOFs） are a unique class of porous crystalline materials that have shown promise for a wide range of applications. MOFs have been explored as a new type of heterogeneous catalytic materials, because of their high surface area, uniform and tunable pores, facile functionalization and incorporation of catalytic active sites. The use of multi-functional sites MOF materials as catalysts for synergistic catalysis and tandem reactions has attracted increasing attention. In this review, we aim to introduce the construction of bi-or multi-functional MOF catalysts with cooperative or cascade functions via post-synthetic modification（PSM）.
文摘Microbes were cultured from the samples at various depths in a weathering profile of RE-bearing granite in Gonghe RE mine, Guangdong Province. The cultured microbes, existing at a depth of 0.2 similar to 12 in and being more plentiful within 3 in in the profile, include bacteria ( Bacillus, Enterobacter, Escherichia, Alkaligenes, Neisseria, Staphylococcus and anaerobic bacteria such as Clostridium), fungi ( Aspergillus niger, Aspergillus flavus, Penicillium, Mucor and Saccharomycete) and actinomyces. Experiments were made under room temperature by using solutions of the cultured microbes and their metabolites, compared with distilled water and the culture solution without microbes, to leach RE from the sample of the weathering crust. The results are shown by the experiments: (1) The mixed microbes cultured from the profile and their metabolites increase the quantity of RE leached from the sample and reduce the pH of the solutions. (2) The ability to leach RE from the sample varies with various microbes, decreasing in a sequence of fungi ( Mucor, saccharomycete, Aspegillus and Penicillium), zymotic bacilli (Enterobacter, Escherichia etc.), Staphylococcus, zymotic Bacillus, actinomyces and Alkaligenes. (3) The RE leached with bacteria is mainly related to the pH value of the solutions influenced by the metabolites of the bacteria; whereas that leached with fungi is mainly related to the adsorption and imbibition of RE by the fungi and the complexing of RE with their metabolites. (4) Compared with that leached with ammonium sulfate, the fractionation of the RE leached with microbes is characterized by higher delta (Ce), lower delta (Eu) and lower ratios of N-La/Sm and N-Gd/Yb . The result of the fractionation of RE accords with the distribution of RE in the various layers of a profile of weathering crust of granite in South China. The experimental results indicate that microbes and their metabolites should play a positive role in the mobilization, migration and fractionation of RE in the weathering crust in South China.
