OBJECTIVE:To analyze the chemical components of Mudan Huaban recipe(牡丹化斑方,MHR)and evaluate its efficacy and possible mechanism in melasma mice.METHODS:The chemical compositions of MHR were determined by the ultra...OBJECTIVE:To analyze the chemical components of Mudan Huaban recipe(牡丹化斑方,MHR)and evaluate its efficacy and possible mechanism in melasma mice.METHODS:The chemical compositions of MHR were determined by the ultra-high performance liquid chromatography coupled with quadrupole-exactive mass spectrometry method.Female C57BL/6 mice were exposed to ultraviolet B and progesterone for 21 d to induce melasma,and Fontana-Masson staining was used to assess the effects of MHR on melasma.Luteinizing hormone(LH),estradiol,and follicle-stimulating hormone levels were detected by enzyme-linked immunosorbent assay.The superoxide dismutase(SOD)activity and malonic dialdehyde content were detected by chemiluminescence.Tyrosinase and related proteins expressions were detected by Western blots and immunohistochemistry.RESULTS:A total of 43 chemical components were identified in MHR,MHR significantly decreased the melanin particles of melasma mice.MHR treatment significantly reversed the high contents of LH and low activity of SOD in models.MHR significantly reduced the higher levels of tyrosinase,tyrosinase-related proteins-1(TRP-1),TRP-2,microphthalmia-associated transcription factor and phosphorylation of cyclic adenosine monophosphate response element-binding protein(pCREB)/CREB in the skin of melasma mice.CONCLUSIONS:MHR protects against melasma via regulation of sex hormones,oxidative stress,and melanogenesis-related proteins,suggesting its possible use as a supplement and alternative drug therapy for melasma.展开更多
[Objectives]To treat patients with Lower-Jiao Obstruction Syndrome caused by severe pneumonia complicated with heart failure using the retention enema with Modified Dahuang Mudan Decoction,and to observe the effect an...[Objectives]To treat patients with Lower-Jiao Obstruction Syndrome caused by severe pneumonia complicated with heart failure using the retention enema with Modified Dahuang Mudan Decoction,and to observe the effect and safety of this treatment on inflammatory indicators,cardiac function,gastrointestinal function and multiple organ failure in patients with Lower-Jiao(lower energizer)Obstruction Syndrome caused by severe pneumonia complicated with heart failure.[Methods]54 patients with Lower-Jiao Obstruction Syndrome caused by severe pneumonia complicated with heart failure diagnosed in Nanchong Chinese Medicine Hospital from January 2020 to December 2021 were randomly divided into experimental group(n=27)and control group(n=27).The control group was given conventional treatment combined with 0.9%sodium chloride injection(100 mL)+glycerine enema(40 mL).The treatment group was treated with Modified Dahuang Mudan Decoction on the basis of conventional treatment for 7 d.The efficacy indicators and adverse events such as white blood count(WBC),C-reactive protein(CRP),procalcitonin(PCT),N-terminal pro-brain natriuretic peptide(NT-proBNP),Lausanne Intestinal Failure Estimation(LIFE)and multiple organ dysfunction score(MODS)were observed.[Results](i)There was no significant difference in WBC,PCT,CRP and NT-proBNP between the two groups before treatment.All indicators after treatment were significantly lower than those before treatment in both groups(P<0.05).The levels of WBC,PCT and CRP in the treatment group were lower than those in the control group after treatment(P<0.05),and there was no significant difference in NT-proBNP between the two groups after treatment(P>0.05),but the difference in NT-proBNP before and after treatment in the treatment group was higher than that in the control group.(ii)The LIFE of the two groups decreased significantly after treatment,and the improvement of LIFE of the treatment group was better than that of the control group(P<0.05).(iii)The MODS of the two groups decreased significantly after treatment(P<0.05),and the degree of decrease after treatment in the treatment group was higher than that in the control group(P<0.05).(iv)There was no significant difference in all-cause mortality between the two groups(P>0.05).(v)No serious adverse events were found in both groups during the treatment period.[Conclusions]The conventional treatment,combined with the retention enema with Modified Dahuang Mudan Decoction could further reduce the inflammatory indicators,improve cardiac function,improve intestinal function and organ function,and the safety was good.展开更多
Mudan模型(MUltisectoral Development ANalysis of China)是一个中国宏观经济多部门动态模型,它是将投入产出模型和总量计量经济模型有机结合在一起的一种模型,既可用于宏观经济分析,又可将分析深入到产业部门一级,研究各种经济政策和...Mudan模型(MUltisectoral Development ANalysis of China)是一个中国宏观经济多部门动态模型,它是将投入产出模型和总量计量经济模型有机结合在一起的一种模型,既可用于宏观经济分析,又可将分析深入到产业部门一级,研究各种经济政策和外部环境变动对各产业部门的影响。展开更多
目的观察木丹颗粒和复方丹参滴丸治疗2型糖尿病周围神经病变(diabetic peripheral neuropathy,DPN)的临床疗效。方法选取2023年5月—2024年4月在郑州颐和医院、河南省人民医院就诊的DPN患者180例,随机分为木丹颗粒组(木丹颗粒联合硫辛...目的观察木丹颗粒和复方丹参滴丸治疗2型糖尿病周围神经病变(diabetic peripheral neuropathy,DPN)的临床疗效。方法选取2023年5月—2024年4月在郑州颐和医院、河南省人民医院就诊的DPN患者180例,随机分为木丹颗粒组(木丹颗粒联合硫辛酸治疗,85例),复方丹参滴丸组(复方丹参滴丸联合硫辛酸治疗,26例),硫辛酸组(单纯硫辛酸治疗,69例),疗程8周。主要观察指标为三组治疗前后密西根糖尿病周围神经病变评分(michigan diabetic neuropathy score,MDNS);次要观察指标为多伦多临床评分(Toronto clinical scoring system scores,TCSS)、中医临床证候评分(traditional chinese medicine syndrome scores,TCM)及血清超氧化物歧化酶(superoxide dismutase,SOD)水平变化。收集三组患者的年龄、性别比例、病程、血压、体重指数(body mass index,BMI)、合并症(冠心病、高血压、高脂血症)、吸烟饮酒史等临床资料及相关生化指标。结果三组患者MDNS、TCSS、TCM评分总分治疗后均较治疗前降低,木丹颗粒组和复方丹参滴丸组治疗后评分低于硫辛酸组,差异有统计学意义(P<0.05),木丹颗粒组和复方丹参滴丸组比较差异无统计学意义(P>0.05)。木丹颗粒组和复方丹参滴丸组治疗后MDNS、TCSS、TCM评分总有效率(90.58%和88.46%、94.11%和96.15%、92.94%和92.30%)均高于硫辛酸组(66.67%、76.81%、73.91%),差异有统计学意义(P<0.05),但木丹颗粒组和复方丹参滴丸组总有效率比较差异无统计学意义(P>0.05)。三组治疗后血清SOD水平均较治疗前升高,且研究组血清SOD水平高于硫辛酸组,差异有统计学意义(P<0.05)。三组患者病程、血压、BMI、FBG、HbA1c、CHO、TG、HDL-C、LDL-C、ALT和AST等水平无明显差异(P>0.05)。结论木丹颗粒和复方丹参滴丸均可有效改善2型糖尿病周围神经病变,两者和硫辛酸联用疗效相近。展开更多
基金Beijing University of Chinese Medicine Transversal Project:"Internal and External"Product and Technology Development for Herbal Acne and Blemish Removal(No.2019110031001241)the Youth Project Under the National Natural Science Foundation of China:Revealing the Scientific Connotation of Tongfu Chinese Herb Rhubarb in Treating Ischemic Stroke from the Perspective of"Intestinal Tryptophan Metabolism and Central Microglia Polarisation"(No.82104440)。
文摘OBJECTIVE:To analyze the chemical components of Mudan Huaban recipe(牡丹化斑方,MHR)and evaluate its efficacy and possible mechanism in melasma mice.METHODS:The chemical compositions of MHR were determined by the ultra-high performance liquid chromatography coupled with quadrupole-exactive mass spectrometry method.Female C57BL/6 mice were exposed to ultraviolet B and progesterone for 21 d to induce melasma,and Fontana-Masson staining was used to assess the effects of MHR on melasma.Luteinizing hormone(LH),estradiol,and follicle-stimulating hormone levels were detected by enzyme-linked immunosorbent assay.The superoxide dismutase(SOD)activity and malonic dialdehyde content were detected by chemiluminescence.Tyrosinase and related proteins expressions were detected by Western blots and immunohistochemistry.RESULTS:A total of 43 chemical components were identified in MHR,MHR significantly decreased the melanin particles of melasma mice.MHR treatment significantly reversed the high contents of LH and low activity of SOD in models.MHR significantly reduced the higher levels of tyrosinase,tyrosinase-related proteins-1(TRP-1),TRP-2,microphthalmia-associated transcription factor and phosphorylation of cyclic adenosine monophosphate response element-binding protein(pCREB)/CREB in the skin of melasma mice.CONCLUSIONS:MHR protects against melasma via regulation of sex hormones,oxidative stress,and melanogenesis-related proteins,suggesting its possible use as a supplement and alternative drug therapy for melasma.
基金Supported by Sichuan Science and Technology Plan Project-Key R&D Project(2022YFS0410)Nanchong R&D Funding Project(19YFZJ0005)Science and Technology Research Project of Sichuan Provincial Administration of Chinese Medicine(2020LC0150)。
文摘[Objectives]To treat patients with Lower-Jiao Obstruction Syndrome caused by severe pneumonia complicated with heart failure using the retention enema with Modified Dahuang Mudan Decoction,and to observe the effect and safety of this treatment on inflammatory indicators,cardiac function,gastrointestinal function and multiple organ failure in patients with Lower-Jiao(lower energizer)Obstruction Syndrome caused by severe pneumonia complicated with heart failure.[Methods]54 patients with Lower-Jiao Obstruction Syndrome caused by severe pneumonia complicated with heart failure diagnosed in Nanchong Chinese Medicine Hospital from January 2020 to December 2021 were randomly divided into experimental group(n=27)and control group(n=27).The control group was given conventional treatment combined with 0.9%sodium chloride injection(100 mL)+glycerine enema(40 mL).The treatment group was treated with Modified Dahuang Mudan Decoction on the basis of conventional treatment for 7 d.The efficacy indicators and adverse events such as white blood count(WBC),C-reactive protein(CRP),procalcitonin(PCT),N-terminal pro-brain natriuretic peptide(NT-proBNP),Lausanne Intestinal Failure Estimation(LIFE)and multiple organ dysfunction score(MODS)were observed.[Results](i)There was no significant difference in WBC,PCT,CRP and NT-proBNP between the two groups before treatment.All indicators after treatment were significantly lower than those before treatment in both groups(P<0.05).The levels of WBC,PCT and CRP in the treatment group were lower than those in the control group after treatment(P<0.05),and there was no significant difference in NT-proBNP between the two groups after treatment(P>0.05),but the difference in NT-proBNP before and after treatment in the treatment group was higher than that in the control group.(ii)The LIFE of the two groups decreased significantly after treatment,and the improvement of LIFE of the treatment group was better than that of the control group(P<0.05).(iii)The MODS of the two groups decreased significantly after treatment(P<0.05),and the degree of decrease after treatment in the treatment group was higher than that in the control group(P<0.05).(iv)There was no significant difference in all-cause mortality between the two groups(P>0.05).(v)No serious adverse events were found in both groups during the treatment period.[Conclusions]The conventional treatment,combined with the retention enema with Modified Dahuang Mudan Decoction could further reduce the inflammatory indicators,improve cardiac function,improve intestinal function and organ function,and the safety was good.
文摘Mudan模型(MUltisectoral Development ANalysis of China)是一个中国宏观经济多部门动态模型,它是将投入产出模型和总量计量经济模型有机结合在一起的一种模型,既可用于宏观经济分析,又可将分析深入到产业部门一级,研究各种经济政策和外部环境变动对各产业部门的影响。
文摘目的观察木丹颗粒和复方丹参滴丸治疗2型糖尿病周围神经病变(diabetic peripheral neuropathy,DPN)的临床疗效。方法选取2023年5月—2024年4月在郑州颐和医院、河南省人民医院就诊的DPN患者180例,随机分为木丹颗粒组(木丹颗粒联合硫辛酸治疗,85例),复方丹参滴丸组(复方丹参滴丸联合硫辛酸治疗,26例),硫辛酸组(单纯硫辛酸治疗,69例),疗程8周。主要观察指标为三组治疗前后密西根糖尿病周围神经病变评分(michigan diabetic neuropathy score,MDNS);次要观察指标为多伦多临床评分(Toronto clinical scoring system scores,TCSS)、中医临床证候评分(traditional chinese medicine syndrome scores,TCM)及血清超氧化物歧化酶(superoxide dismutase,SOD)水平变化。收集三组患者的年龄、性别比例、病程、血压、体重指数(body mass index,BMI)、合并症(冠心病、高血压、高脂血症)、吸烟饮酒史等临床资料及相关生化指标。结果三组患者MDNS、TCSS、TCM评分总分治疗后均较治疗前降低,木丹颗粒组和复方丹参滴丸组治疗后评分低于硫辛酸组,差异有统计学意义(P<0.05),木丹颗粒组和复方丹参滴丸组比较差异无统计学意义(P>0.05)。木丹颗粒组和复方丹参滴丸组治疗后MDNS、TCSS、TCM评分总有效率(90.58%和88.46%、94.11%和96.15%、92.94%和92.30%)均高于硫辛酸组(66.67%、76.81%、73.91%),差异有统计学意义(P<0.05),但木丹颗粒组和复方丹参滴丸组总有效率比较差异无统计学意义(P>0.05)。三组治疗后血清SOD水平均较治疗前升高,且研究组血清SOD水平高于硫辛酸组,差异有统计学意义(P<0.05)。三组患者病程、血压、BMI、FBG、HbA1c、CHO、TG、HDL-C、LDL-C、ALT和AST等水平无明显差异(P>0.05)。结论木丹颗粒和复方丹参滴丸均可有效改善2型糖尿病周围神经病变,两者和硫辛酸联用疗效相近。
文摘高温烟气颗粒、二氧化碳和水蒸气是烃类池火中火焰热辐射的主要来源,而点源模型、Shokri Beyler模型和Mudan模型是计算池火辐射最常用的方法。采用这3种模型计算了直径为2 m的乙醇池火的火焰特性,得出了目标位置接受辐射强度和与池火中心距离之间的关系。根据乙醇池火试验的光谱辐射强度,以及通过简化处理计算得到的火焰周围大气环境的衰减系数,采用辐射传热理论计算目标位置处的辐射强度。将不同模型的计算结果与基于试验数据的计算结果对比发现:当热辐射强度小于1.5 k W/m2时,点源模型计算结果比较准确;当热辐射强度大于4.5 k W/m2时,Shokri Beyler模型的计算结果比较合理。在工业火灾的实际应用中,计算辐射对人员的伤害可选用点源模型与Mudan模型;计算辐射对周围建筑物的破坏可选用Shokri Beyler模型。
