In order to improve the substrate diffusion properties and stability of an immobilized enzyme alginate microgels modified with TiO2 nanoparticles were employed as the enzyme immobilizing support.Ionotropic gelation wa...In order to improve the substrate diffusion properties and stability of an immobilized enzyme alginate microgels modified with TiO2 nanoparticles were employed as the enzyme immobilizing support.Ionotropic gelation was applied for the preparation of hybrid gels while Ca2＋ Ce3＋ Ni2＋Cu2＋and Fe3＋were employed as the crosslinkers.Papain was selected as the model enzyme. UV-Vis spectroscopy was employed to investigate the activity of papain to evaluate kinetics and stability.Analysis results show that the highest affinity the lowest Michaelis-Menten constant Km =11.0 mg/mL and the highest stability are obtained when using Cu2＋as the crosslinker.The effect of the mass ratio of TiO2 to papain on the stability and leakage of papain is also investigated and the results show that 10∶1 TiO2∶papain is optimal because the proper use of TiO2 can reduce enzyme leakage and ensure enzyme stability.Preparing Cu/alginate/TiO2 hybrid gels via ionotropic gelation can provide a satisfactory diffusion capability and enzyme stability.展开更多
Ni-mSA-mCS bipolar membrane （BM） was prepared by sodium alginate （SA） and chitosan （CS）, which were modified by Ca^2＋ and glutaraldehyde as linking reagents, respectively, mSA-mCS membrane was characterized by ...Ni-mSA-mCS bipolar membrane （BM） was prepared by sodium alginate （SA） and chitosan （CS）, which were modified by Ca^2＋ and glutaraldehyde as linking reagents, respectively, mSA-mCS membrane was characterized by FTIR, SEM, TG and used as a separator in the electrolysis cell to produce thioglycolic acid （TGA）. The experiment results show that TGAwas prepared effectively by electro-reduction of dithiodiglycolic acid （DTDGA） with the mixture of TGA and DTDGA in the cathodic chamber. The current efficiency was up to 66.7% at the room temperature （25 ℃） during the current density of 10 mA/cm^2. Compared with the traditional metal reduction method, the electro-reduction technology saves the zinc powder and eliminates the pollution to environment.展开更多
Flexible zinc-air batteries(ZABs)are highly promising for use in bendable electronic devices,including wearable sensors,bendable smartphones,and flexible displays,owing to their exceptional theoretical energy density,...Flexible zinc-air batteries(ZABs)are highly promising for use in bendable electronic devices,including wearable sensors,bendable smartphones,and flexible displays,owing to their exceptional theoretical energy density,affordability,and safety features.Nevertheless,conventional ZABs rely on liquid electrolytes,which present certain limitations such as electrolyte leakage,severely restricting their practical application.Consequently,developing a high-performance,high-safety,and environmentally degradable solid-state electrolyte(SSE)has emerged as a focal point in current research endeavors.In this work,we designed and fabricated an SSE based on modified sodium alginate with exceptional water absorption capacity,biodegradability,and ion conductivity of up to 0.231 S cm^(-1).The water-retentive sodium alginate/ion-conductive sodium alginate(WSA/ISA)-based ZAB delivered an impressive open-circuit voltage of 1.54 V and a peak power density of 63 mWcm^(-2),with stable output under various bending states.This research offers fresh perspectives on the development and fabrication of highefficiency,eco-friendly,and flexible ZAB SSEs.展开更多
基金The National Natural Science Foundation of China(No.21005016)the Foundation of Educational Commission of Jiangsu Province(No.JHB2011-2)
文摘In order to improve the substrate diffusion properties and stability of an immobilized enzyme alginate microgels modified with TiO2 nanoparticles were employed as the enzyme immobilizing support.Ionotropic gelation was applied for the preparation of hybrid gels while Ca2＋ Ce3＋ Ni2＋Cu2＋and Fe3＋were employed as the crosslinkers.Papain was selected as the model enzyme. UV-Vis spectroscopy was employed to investigate the activity of papain to evaluate kinetics and stability.Analysis results show that the highest affinity the lowest Michaelis-Menten constant Km =11.0 mg/mL and the highest stability are obtained when using Cu2＋as the crosslinker.The effect of the mass ratio of TiO2 to papain on the stability and leakage of papain is also investigated and the results show that 10∶1 TiO2∶papain is optimal because the proper use of TiO2 can reduce enzyme leakage and ensure enzyme stability.Preparing Cu/alginate/TiO2 hybrid gels via ionotropic gelation can provide a satisfactory diffusion capability and enzyme stability.
基金supported by the Nature Science Foundations of Fujian Province(No.D0710009)the Fujian Education Bureau(Nos.JB06069,JB05314).
文摘Ni-mSA-mCS bipolar membrane （BM） was prepared by sodium alginate （SA） and chitosan （CS）, which were modified by Ca^2＋ and glutaraldehyde as linking reagents, respectively, mSA-mCS membrane was characterized by FTIR, SEM, TG and used as a separator in the electrolysis cell to produce thioglycolic acid （TGA）. The experiment results show that TGAwas prepared effectively by electro-reduction of dithiodiglycolic acid （DTDGA） with the mixture of TGA and DTDGA in the cathodic chamber. The current efficiency was up to 66.7% at the room temperature （25 ℃） during the current density of 10 mA/cm^2. Compared with the traditional metal reduction method, the electro-reduction technology saves the zinc powder and eliminates the pollution to environment.
基金the National Natural Science Foundation of China(grant no.22209150)Zhejiang Normal University Scientific Research Foundation,China(grant nos.YS304221906 and YS304022904)+2 种基金the Power Battery and Systems Research Center from State Key Laboratory of Catalysis,Strategic Priority Research Program of Chinese Academy of Sciences(grant no.XDB0600104)Dalian Revitalization Talents Program,Liaoning,China(grant no.2022RG01)Liaoning Binhai Laboratory Grant,China(grant no.LBLB202304).
文摘Flexible zinc-air batteries(ZABs)are highly promising for use in bendable electronic devices,including wearable sensors,bendable smartphones,and flexible displays,owing to their exceptional theoretical energy density,affordability,and safety features.Nevertheless,conventional ZABs rely on liquid electrolytes,which present certain limitations such as electrolyte leakage,severely restricting their practical application.Consequently,developing a high-performance,high-safety,and environmentally degradable solid-state electrolyte(SSE)has emerged as a focal point in current research endeavors.In this work,we designed and fabricated an SSE based on modified sodium alginate with exceptional water absorption capacity,biodegradability,and ion conductivity of up to 0.231 S cm^(-1).The water-retentive sodium alginate/ion-conductive sodium alginate(WSA/ISA)-based ZAB delivered an impressive open-circuit voltage of 1.54 V and a peak power density of 63 mWcm^(-2),with stable output under various bending states.This research offers fresh perspectives on the development and fabrication of highefficiency,eco-friendly,and flexible ZAB SSEs.
