Drought stress significantly impedes apple growth,development,and yield,leading to substantial economic losses within the global apple industry.Malus prunifolia(Mp),a commonly utilized apple rootstock,has shown promis...Drought stress significantly impedes apple growth,development,and yield,leading to substantial economic losses within the global apple industry.Malus prunifolia(Mp),a commonly utilized apple rootstock,has shown promise in augmenting cultivated apple resistance to abiotic stress.Although Alfin-like(ALs)proteins have demonstrated pivotal roles in dicotyledonous plants'response to abiotic stresses,knowledge about AL genes in apple rootstocks is limited,and their functions remain largely elusive.In this study,we identified and characterized 10 MpAL gene members in the apple rootstock genome,confirming their localization within the nucleus.Our investigation revealed the significant regulation of MpALs'expression under drought and abscisic acid(ABA)stresses in M.prunifolia.In this study,one of the members,MpAL1,was selected for further exploration in Arabidopsis and apple to explore its potential function in response to drought and ABA stresses.The results showed that overexpression-MpAL1 transgenic apple calli grew significantly better than WT and MpAL1-RNAi lines,which regulates the accumulation of H_(2)O_(2)and O_(2).-levels.Additionally,transgenic Arabidopsis plants overexpressing MpAL1 exhibited positively regulating antioxidant enzymes activities under stress treatments.Further study showed that silencing MpAL1 in apple plants showed obvious chlorosis in leaves,and accumulation of reactive oxygen species under drought stress.Moreover,our detailed analysis established that MpAL1 regulates several drought and ABA-responsive genes,exerting an influence on their expression in transgenic apple.Collectively,our findings identify MpAL1 as a positive regulator that increases drought stress in apple,shedding light on its potential significance in bolstering drought resistance in this fruit crop.展开更多
Biphenotypic acute leukemia (BAL) is an uncommon clinical entity. It is a type of acute leukemia with features characteristic of both the myeloid and lymphoid lineages and for this reason is designated as mixed-lineag...Biphenotypic acute leukemia (BAL) is an uncommon clinical entity. It is a type of acute leukemia with features characteristic of both the myeloid and lymphoid lineages and for this reason is designated as mixed-lineage, hybrid or biphenotypic acute leukemia. As strict diagnostic criteria have only recently been established, the precise incidence among acute leukemia is uncertain, although it is likely to account for approximately less then 5% of all acute leukemia. BAL is now collectively considered as “mixed phenotype acute leukemia” (MPAL). We hereby report two cases of a rare disease, BAL from our institution in the light of morphology, cytochemistry, flow cytometry and review of literature regarding these cases are described.展开更多
Objective To evaluate the safety and efficacy of mitoxantrone liposome(MIT-LIP)combined chemotherapy in treating mixed phenotype acute leukemia(MPAL).Methods December 2021 to November 2024,MPAL patients who underwent ...Objective To evaluate the safety and efficacy of mitoxantrone liposome(MIT-LIP)combined chemotherapy in treating mixed phenotype acute leukemia(MPAL).Methods December 2021 to November 2024,MPAL patients who underwent the MAED(MIT-LIP+cytarabine+etoposide+dexamethasone)regimen were retrospectively analyzed.Data on clinical characteristics,adverse reactions,therapeutic outcomes,and long-term prognoses were collected.Results A total of 7 MPAL patients who received MAED regimen were admitted.Among them,two patients were initially diagnosed with T-ALL or BALL,respectively,and transformed into AML after treatment.Three patients were initially diagnosed as MPAL(B/myeloid),one as MPAL(T/myeloid),and one with MPAL(myeloid/plasmacytoid dendritic cell).Among the 7 patients,there were 3 males and 4 females,1 chromosome abnormalities and 6 gene abnormalities,including 1 case with BCR::ABL fusion gene.The median age was 38 years(range:16-58 years).There was no clear related drug allergy and organ toxicity during MAED regimen,and the main adverse effect was hematological toxicity.After induced chemotherapy,all patientsachieved complete remission(CR),2 maintained MRD-negative CR and 1 maintained MRDpositive CR.The other 4 patients underwent allogeneic hematopoietic stem cell transplantation,2 maintained MRD-negative CR,and 2 relapsed.The current median follow-up time was 12 months,the overall survival(OS)rate was 100%,the relapse-free survival(RFS)rate was 60%,and the median OS time and median RFS time were not reached.Conclusion The MAEDDregimen demonstrates high safety and a favorable CR rate in MPAL treatment.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.32102311 and 32102338)the China Postdoctoral Science Foundation(Grant No.2021M690129).
文摘Drought stress significantly impedes apple growth,development,and yield,leading to substantial economic losses within the global apple industry.Malus prunifolia(Mp),a commonly utilized apple rootstock,has shown promise in augmenting cultivated apple resistance to abiotic stress.Although Alfin-like(ALs)proteins have demonstrated pivotal roles in dicotyledonous plants'response to abiotic stresses,knowledge about AL genes in apple rootstocks is limited,and their functions remain largely elusive.In this study,we identified and characterized 10 MpAL gene members in the apple rootstock genome,confirming their localization within the nucleus.Our investigation revealed the significant regulation of MpALs'expression under drought and abscisic acid(ABA)stresses in M.prunifolia.In this study,one of the members,MpAL1,was selected for further exploration in Arabidopsis and apple to explore its potential function in response to drought and ABA stresses.The results showed that overexpression-MpAL1 transgenic apple calli grew significantly better than WT and MpAL1-RNAi lines,which regulates the accumulation of H_(2)O_(2)and O_(2).-levels.Additionally,transgenic Arabidopsis plants overexpressing MpAL1 exhibited positively regulating antioxidant enzymes activities under stress treatments.Further study showed that silencing MpAL1 in apple plants showed obvious chlorosis in leaves,and accumulation of reactive oxygen species under drought stress.Moreover,our detailed analysis established that MpAL1 regulates several drought and ABA-responsive genes,exerting an influence on their expression in transgenic apple.Collectively,our findings identify MpAL1 as a positive regulator that increases drought stress in apple,shedding light on its potential significance in bolstering drought resistance in this fruit crop.
文摘Biphenotypic acute leukemia (BAL) is an uncommon clinical entity. It is a type of acute leukemia with features characteristic of both the myeloid and lymphoid lineages and for this reason is designated as mixed-lineage, hybrid or biphenotypic acute leukemia. As strict diagnostic criteria have only recently been established, the precise incidence among acute leukemia is uncertain, although it is likely to account for approximately less then 5% of all acute leukemia. BAL is now collectively considered as “mixed phenotype acute leukemia” (MPAL). We hereby report two cases of a rare disease, BAL from our institution in the light of morphology, cytochemistry, flow cytometry and review of literature regarding these cases are described.
文摘Objective To evaluate the safety and efficacy of mitoxantrone liposome(MIT-LIP)combined chemotherapy in treating mixed phenotype acute leukemia(MPAL).Methods December 2021 to November 2024,MPAL patients who underwent the MAED(MIT-LIP+cytarabine+etoposide+dexamethasone)regimen were retrospectively analyzed.Data on clinical characteristics,adverse reactions,therapeutic outcomes,and long-term prognoses were collected.Results A total of 7 MPAL patients who received MAED regimen were admitted.Among them,two patients were initially diagnosed with T-ALL or BALL,respectively,and transformed into AML after treatment.Three patients were initially diagnosed as MPAL(B/myeloid),one as MPAL(T/myeloid),and one with MPAL(myeloid/plasmacytoid dendritic cell).Among the 7 patients,there were 3 males and 4 females,1 chromosome abnormalities and 6 gene abnormalities,including 1 case with BCR::ABL fusion gene.The median age was 38 years(range:16-58 years).There was no clear related drug allergy and organ toxicity during MAED regimen,and the main adverse effect was hematological toxicity.After induced chemotherapy,all patientsachieved complete remission(CR),2 maintained MRD-negative CR and 1 maintained MRDpositive CR.The other 4 patients underwent allogeneic hematopoietic stem cell transplantation,2 maintained MRD-negative CR,and 2 relapsed.The current median follow-up time was 12 months,the overall survival(OS)rate was 100%,the relapse-free survival(RFS)rate was 60%,and the median OS time and median RFS time were not reached.Conclusion The MAEDDregimen demonstrates high safety and a favorable CR rate in MPAL treatment.
