目的:探讨乳腺癌MDA-MB-231细胞中,Y性别决定区基因7(SOX7)基因启动子甲基化水平对细胞的体外迁移和侵袭的影响。方法:脂质体转染pcDNA3.0-DNA甲基转移酶3a(DNMT3a)质粒至MDA-MB-231细胞中,并于24h、48h及72h后,采用蛋白质免疫印迹实验(...目的:探讨乳腺癌MDA-MB-231细胞中,Y性别决定区基因7(SOX7)基因启动子甲基化水平对细胞的体外迁移和侵袭的影响。方法:脂质体转染pcDNA3.0-DNA甲基转移酶3a(DNMT3a)质粒至MDA-MB-231细胞中,并于24h、48h及72h后,采用蛋白质免疫印迹实验(WB)检测细胞内DNMT3a蛋白表达水平;甲基化特异性定量PCR(Q-MSP)检测DNMT3a处理组、5-aza-C处理组及对照(Control)组MDA-MB-231细胞中的SOX7基因启动子DNA甲基化水平;实时荧光定量PCR(qRT-PCR)及WB实验检测各组MDA-MB-231细胞中的SOX7 m RNA和蛋白表达水平;细胞划痕实验及细胞侵袭实验检测各组MDA-MB-231细胞的迁移和侵袭能力。结果:pcDNA3.0-DNMT3a质粒转染MDA-MB-231细胞24h时,细胞内的DNMT3a蛋白表达水平最高。DNMT3a能够显著提高SOX7基因启动子DNA甲基化水平,而5-aza-C则抑制了SOX7基因启动子DNA甲基化水平(P<0.05)。与Control组相比,DNMT3a处理组的MDA-MB-231细胞中,SOX7的m RNA及蛋白表达水平均明显下降,而5-aza-C处理组SOX7的m RNA及蛋白表达水平均明显增加(P<0.05)。与Control组相比,DNMT3a处理组的MDA-MB-231细胞的迁移和侵袭能力均显著增强(P<0.05),而5-aza-C处理组的MDA-MB-231细胞的迁移和侵袭能力变化不大(P>0.05)。结论:在恶性肿瘤中,SOX7低表达表受其基因启动子高甲基化调节,且乳腺癌MDA-MB-231细胞中低表达的SOX7能够影响细胞的外迁移和侵袭能力。展开更多
Dermatitis is a combination of various skin disease agents such as bacteria,fungi,ectoparasites,viruses and metabolic diseases with various clinical symptoms or signs.Dermatitis can cause an imbalance between ROS and ...Dermatitis is a combination of various skin disease agents such as bacteria,fungi,ectoparasites,viruses and metabolic diseases with various clinical symptoms or signs.Dermatitis can cause an imbalance between ROS and antioxidants.Exogenous antioxidants are needed to help balance antioxidant and ROS levels in the body by providing trigona honey.This study aims to determine the MDA and SOD levels of dogs with complex dermatitis after administration of trigona honey.This study used 12 dogs with complex dermatitis,with a moderate severity score.Dogs 4-6 months old.Samples were divided into control group(P0)without honey,fresh honey group(P1)5 mL/head/day and capsule honey group(P2)0.1 mg/head/day.Honey was given for 4 weeks in the treatment group.Examination of MDA and SOD levels was carried out using the ELISA kit.The results showed that the Trigona honey treatment had a significant effect(P<0.05)in reducing MDA levels and increasing SOD levels in dogs with complex dermatitis.Duncan's test results showed that SOD levels increased significantly at week 4,while week 2 and week 0 did not increase significantly.Trigona honey has anti-inflammatory,antibacterial,antiaging,antiulcer effects,helps in wound healing dermatitis and is an antioxidant.So that trigona honey can reduce plasma MDA levels and increase plasma SOD levels in dogs with complex dermatitis.展开更多
文摘目的:探讨乳腺癌MDA-MB-231细胞中,Y性别决定区基因7(SOX7)基因启动子甲基化水平对细胞的体外迁移和侵袭的影响。方法:脂质体转染pcDNA3.0-DNA甲基转移酶3a(DNMT3a)质粒至MDA-MB-231细胞中,并于24h、48h及72h后,采用蛋白质免疫印迹实验(WB)检测细胞内DNMT3a蛋白表达水平;甲基化特异性定量PCR(Q-MSP)检测DNMT3a处理组、5-aza-C处理组及对照(Control)组MDA-MB-231细胞中的SOX7基因启动子DNA甲基化水平;实时荧光定量PCR(qRT-PCR)及WB实验检测各组MDA-MB-231细胞中的SOX7 m RNA和蛋白表达水平;细胞划痕实验及细胞侵袭实验检测各组MDA-MB-231细胞的迁移和侵袭能力。结果:pcDNA3.0-DNMT3a质粒转染MDA-MB-231细胞24h时,细胞内的DNMT3a蛋白表达水平最高。DNMT3a能够显著提高SOX7基因启动子DNA甲基化水平,而5-aza-C则抑制了SOX7基因启动子DNA甲基化水平(P<0.05)。与Control组相比,DNMT3a处理组的MDA-MB-231细胞中,SOX7的m RNA及蛋白表达水平均明显下降,而5-aza-C处理组SOX7的m RNA及蛋白表达水平均明显增加(P<0.05)。与Control组相比,DNMT3a处理组的MDA-MB-231细胞的迁移和侵袭能力均显著增强(P<0.05),而5-aza-C处理组的MDA-MB-231细胞的迁移和侵袭能力变化不大(P>0.05)。结论:在恶性肿瘤中,SOX7低表达表受其基因启动子高甲基化调节,且乳腺癌MDA-MB-231细胞中低表达的SOX7能够影响细胞的外迁移和侵袭能力。
文摘Dermatitis is a combination of various skin disease agents such as bacteria,fungi,ectoparasites,viruses and metabolic diseases with various clinical symptoms or signs.Dermatitis can cause an imbalance between ROS and antioxidants.Exogenous antioxidants are needed to help balance antioxidant and ROS levels in the body by providing trigona honey.This study aims to determine the MDA and SOD levels of dogs with complex dermatitis after administration of trigona honey.This study used 12 dogs with complex dermatitis,with a moderate severity score.Dogs 4-6 months old.Samples were divided into control group(P0)without honey,fresh honey group(P1)5 mL/head/day and capsule honey group(P2)0.1 mg/head/day.Honey was given for 4 weeks in the treatment group.Examination of MDA and SOD levels was carried out using the ELISA kit.The results showed that the Trigona honey treatment had a significant effect(P<0.05)in reducing MDA levels and increasing SOD levels in dogs with complex dermatitis.Duncan's test results showed that SOD levels increased significantly at week 4,while week 2 and week 0 did not increase significantly.Trigona honey has anti-inflammatory,antibacterial,antiaging,antiulcer effects,helps in wound healing dermatitis and is an antioxidant.So that trigona honey can reduce plasma MDA levels and increase plasma SOD levels in dogs with complex dermatitis.
