Background: Resveratrol is a widely recognized anti-inflammatory and antioxidant agent,and it has been suggested to possess anti-tumor effects. But the effect of resveratrol onhepatocellular carcinoma and its molecula...Background: Resveratrol is a widely recognized anti-inflammatory and antioxidant agent,and it has been suggested to possess anti-tumor effects. But the effect of resveratrol onhepatocellular carcinoma and its molecular mechanisms are unknown. This study confirmedthe effects of resveratrol on HepG2 cell proliferation and migration, and the underlyingmechanism. Methods: Viability of resveratrol (0-200 μmol/L)-treated HepG2 cells wasdetected by CCK-8. Wound healing assay was employed to evaluate cell migration. Theexpression levels of proteins including Bcl-2, Bax, Caspase3, SIRT1, and components of theMAPK pathway were analyzed via Western blot. Results: Resveratrol significantly inhibitedthe migration and proliferation of HepG2 cells at concentrations above 100 μmol/L(P<0.01). The expression of Bax, cleaved Caspase3 and SIRT1 was up-regulate (P<0.05)and Bcl-2, p-JNK、p-p38 MAPK was down-regulate (P<0.05) by resveratrol. Conclusion:Resveratrol suppresses the proliferation and migration of HepG2 cells by activating theSIRT1 signaling pathway and inhibiting the JNK and p38 MAPK pathways.展开更多
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection disrupts immune function by activating inflammatory pathways related to disease severity.Understanding virus-induced inflammation is crucial for dev...Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection disrupts immune function by activating inflammatory pathways related to disease severity.Understanding virus-induced inflammation is crucial for developing anti-coronavirus disease 2019(COVID-19)therapies.This study used principal component analysis,heatmaps,and other tools to examine mitogen-activated protein kinase(MAPK)pathway gene expression,and found that alterations in MAPK pathway genes were correlated with immune response changes.Further analysis linked P38-related gene expression to clinical symptoms,with transcriptomic data showing a strong association between MAPK gene expression changes and SARS-CoV-2 infection.In infected cell models,phosphorylated P38(P-P38)protein and inflammatory factors were significantly upregulated.Analysis of the GSE217948 dataset showed a significant correlation between plasma markers(interferon inducible protein-10(IP-10)and interleukin(IL)-6)and symptoms(fever and fatigue).Activation of P38 appears to release inflammatory factors tied to these symptoms making P38 as a key pathway in virus-induced inflammation.Forsythin(KD-1),an anti-inflammatory compound from forsythia showed efficacy against SARS-CoV-2,inhibiting replication,reducing P38 levels,and lowering inflammatory markers(IL-6,IL-8,IP-10,tumor necrosis factor-α(TNF-α),and monocyte chemotactic protein-1(MCP-1))in both cell and animal models.In specific cell models,KD-1 blocked P38 activation,thereby reducing inflammation.In a P38 overexpression model,KD-1 decreased P38 phosphorylation and downstream inflammatory proteins.This study identifies the P38 pathway as a therapeutic target for COVID-19,supporting KD-1’s potential in mitigating virus-induced inflammation and guiding further research into anti-inflammatory treatments for respiratory viruses.展开更多
基金supported by Natural Science Foundation of Nanjing University of Chinese Medicine(XZR2023010)Traditional Chinese Medicine Science and Technology Project of Jiangsu Province(QN202212)Guidance Program of Jiangsu Commission of Health(Z2020046).
文摘Background: Resveratrol is a widely recognized anti-inflammatory and antioxidant agent,and it has been suggested to possess anti-tumor effects. But the effect of resveratrol onhepatocellular carcinoma and its molecular mechanisms are unknown. This study confirmedthe effects of resveratrol on HepG2 cell proliferation and migration, and the underlyingmechanism. Methods: Viability of resveratrol (0-200 μmol/L)-treated HepG2 cells wasdetected by CCK-8. Wound healing assay was employed to evaluate cell migration. Theexpression levels of proteins including Bcl-2, Bax, Caspase3, SIRT1, and components of theMAPK pathway were analyzed via Western blot. Results: Resveratrol significantly inhibitedthe migration and proliferation of HepG2 cells at concentrations above 100 μmol/L(P<0.01). The expression of Bax, cleaved Caspase3 and SIRT1 was up-regulate (P<0.05)and Bcl-2, p-JNK、p-p38 MAPK was down-regulate (P<0.05) by resveratrol. Conclusion:Resveratrol suppresses the proliferation and migration of HepG2 cells by activating theSIRT1 signaling pathway and inhibiting the JNK and p38 MAPK pathways.
基金supported by the National Administration of Traditional Chinese Medicine(ZYYCXTU-D-202201 and ZYYCXTU-D-202206)the National Natural Science Foundation of China(82474155,82174053,82141201,and 82341099)+3 种基金the Guangdong Basic and Applied Basic Research Foundation(2022A1515010301)the Macao Science and Technology Development Fund(0022/2021/A1)the Young Top Talent of Science and Technology Innovation Department of Guangdong Province(2021TQ060189)the Youth Lift Project of China Association for Science and Technology(2020-2022QNRC001).
文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection disrupts immune function by activating inflammatory pathways related to disease severity.Understanding virus-induced inflammation is crucial for developing anti-coronavirus disease 2019(COVID-19)therapies.This study used principal component analysis,heatmaps,and other tools to examine mitogen-activated protein kinase(MAPK)pathway gene expression,and found that alterations in MAPK pathway genes were correlated with immune response changes.Further analysis linked P38-related gene expression to clinical symptoms,with transcriptomic data showing a strong association between MAPK gene expression changes and SARS-CoV-2 infection.In infected cell models,phosphorylated P38(P-P38)protein and inflammatory factors were significantly upregulated.Analysis of the GSE217948 dataset showed a significant correlation between plasma markers(interferon inducible protein-10(IP-10)and interleukin(IL)-6)and symptoms(fever and fatigue).Activation of P38 appears to release inflammatory factors tied to these symptoms making P38 as a key pathway in virus-induced inflammation.Forsythin(KD-1),an anti-inflammatory compound from forsythia showed efficacy against SARS-CoV-2,inhibiting replication,reducing P38 levels,and lowering inflammatory markers(IL-6,IL-8,IP-10,tumor necrosis factor-α(TNF-α),and monocyte chemotactic protein-1(MCP-1))in both cell and animal models.In specific cell models,KD-1 blocked P38 activation,thereby reducing inflammation.In a P38 overexpression model,KD-1 decreased P38 phosphorylation and downstream inflammatory proteins.This study identifies the P38 pathway as a therapeutic target for COVID-19,supporting KD-1’s potential in mitigating virus-induced inflammation and guiding further research into anti-inflammatory treatments for respiratory viruses.
