Siraitia grosvenorii(SG),a traditional edible medicine in China,has shown potential application in health foods due to multiple bioactivities.However,few comparative studies have investigated the effect of daily intak...Siraitia grosvenorii(SG),a traditional edible medicine in China,has shown potential application in health foods due to multiple bioactivities.However,few comparative studies have investigated the effect of daily intake of SG products by traditional drinking way on alleviating metabolic disorders caused by long-term high fat and high sugar diet(HF-HSD).This study investigated the effects of 2 commercial SG products(SG juice containing comprehensive compositions and mogrosides extract containing high content of mogroside V)supplement on fat content,lipid accumulation,colon barrier and gut microbiota in a 38-week HF-HSD mice trial.Results showed that the SG intervention could decrease the weight gain and body fat content,alleviated lipid accumulation,low-grade inflammation and colon barrier injury.SG juice intake was found to increase the antioxidant enzyme activities of serum glutathione peroxidase and liver superoxide dismutase.16S r RNA analysis found that SG could restore the gut microbiota with downregulation of harmful bacteria increased by HF-HSD and enhancement of the abundances of beneficial genus.In particular,SG juice significantly enriched taxonomic family Prevotellaceae and genus Alloprevotella.These results indicated that SG might be a functional additive to prevent disease risk caused by excess fat and sugar diet,and active ingredients resulting to the discrepancy of performance especially on gut microbiota are worthy of in-depth investigation.展开更多
Obesity has brought great challenges to global human health,and how to effectively prevent and control the occurrence and development of obesity has become an urgent problem.The role and mechanism of 4-[(α-Lrhamnosyl...Obesity has brought great challenges to global human health,and how to effectively prevent and control the occurrence and development of obesity has become an urgent problem.The role and mechanism of 4-[(α-Lrhamnosyloxy)benzyl]isothiocyanate(MITC),an active ingredient of Moringa oleifera Lam.,in the regulation of lipid metabolism have not been comprehensively investigated.In the present study,we investigated the mechanism of MITC in inhibiting lipid accumulation in mice fed with a high-fat diet(HFD)in terms of both lipolysis and central appetite regulation mediated by the gut microbe-gut-brain axis.MITC enhanced the characteristic indices associated with HFD mice and also promoted adipocytolysis and brown fat thermogenesis.Moreover,MITC was observed to improve leptin resistance,modulate the composition of gut microbiota such as Ruminococcaceae,Parasutterella,and Acetatifactor,promote 5-hydroxytryptamine secretion,further enhance the secretion of glucagon-like peptide-1(GLP-1)and peptide tyrosine-tyrosine(PYY)to activate peroxisome proliferator-activated receptor(PPAR)signaling in the hypothalamus,and modulate feeding behavior to inhibit lipid accumulation in HFD mice.These data suggest that MITC supplementation can help to alleviate obesity or obesity-related diseases.展开更多
Objective:To investigate the effects of Alpiniae oxyphyllae Fructus(AOF)on renal lipid deposition in diabetic kidney disease(DKD)and elucidate its molecular mechanisms.Methods:The mechanism of AOF in treating DKD was ...Objective:To investigate the effects of Alpiniae oxyphyllae Fructus(AOF)on renal lipid deposition in diabetic kidney disease(DKD)and elucidate its molecular mechanisms.Methods:The mechanism of AOF in treating DKD was explored by network pharmacological enrichment analysis,molecular docking,and molecular dynamics simulation.The effects of AOF on renal function and lipid deposition were assessed in a mouse model of DKD and high glucose-stressed HK-2 cells.Cell viability and lipid accumulation were detected by CCK8 and oil red O staining.The expressions of PPARαand fatty acid oxidation-related genes(ACOX1 and CPT1A)were detected by quantitative RT-PCR,Western blot,and immunofluorescence.Furthermore,PPARαknockdown was performed to examine the molecular mechanism of AOF in treating DKD.Results:Network pharmacological enrichment analysis,molecular docking,and molecular dynamics simulation showed that the active compounds in AOF targeted PPARαand thus transcriptionally regulated ACOX1 and CPT1A.AOF lowered blood glucose,improved dyslipidemia,and attenuated renal injury in DKD mice.AOF-containing serum accentuated high glucose-induced decrease in cell viability and ameliorated lipid accumulation.Additionally,it significantly upregulated the expression of PPARα,ACOX1,and CPT1A in both in vivo and in vitro experiments,which was reversed by PPARαknockdown.Conclusions:AOF may promote fatty acid oxidation via PPARαto ameliorate renal lipid deposition in DKD.展开更多
BACKGROUND Metabolic dysfunction-associated steatotic liver disease(MASLD)has emerged as a prominent and pervasive global health challenge.Bicuculline(BIC),which is a key active component of the anti-MASLD prescriptio...BACKGROUND Metabolic dysfunction-associated steatotic liver disease(MASLD)has emerged as a prominent and pervasive global health challenge.Bicuculline(BIC),which is a key active component of the anti-MASLD prescription"Eight Zhes Decoction",has been preliminarily shown by our research team to have significant potential in treating MASLD.AIM To determine BIC's efficacy in treating MASLD by regulating lipid metabolism and suppressing hepatic inflammation via nuclear factor-kappa B(NF-κB)pathway,identifying it as a therapeutic candidate.METHODS This study explored the potential of BIC in preventing and treating MASLD using zebrafish,cellular(HepG2 and AML12),and mouse models.RESULTS Our results indicate that BIC significantly reduces lipid accumulation and inflammation both in vivo and in vitro.Transcriptomic analysis suggested that the anti-MASLD effects of BIC are linked to the inhibition of the NF-κB pathway,which plays a critical role in mitigating inflammation and lipid deposition.CONCLUSION This study is the first to demonstrate that BIC specifically alleviates lipid accumulation and hepatic steatosis in MASLD models via the NF-κB signaling pathway.Overall,BIC has emerged as a promising candidate for treating MASLD.展开更多
Alcoholic liver disease(ALD)is a growing global health concern,and its early pathogenesis includes steatosis and steatohepatitis.Inhibiting lipid accumulation and inflammation is a crucial step in relieving ALD.Eviden...Alcoholic liver disease(ALD)is a growing global health concern,and its early pathogenesis includes steatosis and steatohepatitis.Inhibiting lipid accumulation and inflammation is a crucial step in relieving ALD.Evidence shows that puerarin(Pue),an isoflavone isolated from Pueraria lobata,exerts cardio-protective,neuroprotective,anti-inflammatory,antioxidant activities.However,the therapeutic potential of Pue on ALD remains unknown.In the study,both the NIAAA model and ethanol(EtOH)-induced AML-12 cell were used to explore the protective effect of Pue on alcoholic liver injury in vivo and in vitro and related mechanism.The results showed that Pue(100 mg·kg^(−1))attenuated EtOH-induced liver injury and inhibited the levels of SREBP-1c,TNF-α,IL-6 and IL-1β,compared with silymarin(Sil,100 mg·kg^(−1)).In vitro results were consistent with in vivo results.Mechanistically,Pue might suppress liver lipid accumulation and inflammation by regulating MMP8.In conclusion,Pue might be a promising clinical candidate for ALD treatment.展开更多
Phosphoenolpyruvate carboxylase（PEPC;EC 4.1.1.31） catalyses phosphoenolpyruvate（PEP） to yield oxaloacetate,which is involved in protein biosynthesis.Pyruvate kinase（PK;EC 2.7.1.40） catalyzes PEP to yield pyruvat...Phosphoenolpyruvate carboxylase（PEPC;EC 4.1.1.31） catalyses phosphoenolpyruvate（PEP） to yield oxaloacetate,which is involved in protein biosynthesis.Pyruvate kinase（PK;EC 2.7.1.40） catalyzes PEP to yield pyruvate,which is involved in fatty acid synthesis.In this study,five PEPC genes（AhPEPC1,AhPEPC2,AhPEPC3,AhPEPC4,and AhPEPC5） from peanut have been cloned.Using a quantitative real-time RT-PCR approach,the expression pattern of each gene was monitored during the seed development of four peanut varieties（E11,Hebeigaoyou,Naihan 1,and Huayu 26）.It was found that these five genes shared similar expression behaviors over the developmental stages of E11 with high expression levels at 30 and 40 d after pegging（DAP）;whereas these five genes showed irregular expression patterns during the seed development of Hebeigaoyou.In Naihan 1 and Huayu 26,the expression levels of the five genes remained relatively high in the first stage.The PEPC activity was monitored during the seed development of four peanut varieties and seed oil content was also characterized during whole period of seed development.The PEPC activity followed the oil accumulation pattern during the early stages of development but they showed a significantly negative correlation thereafter.These results suggested that PEPC may play an important role in lipid accumulation during the seed development of four peanut varieties tested.展开更多
Microalgal lipids are regarded as main future feedstock of biofuels for its higher efficiency of accumulation and sus- tainable production. In order to investigate the effect of various nitrogen to phosphorus ratios o...Microalgal lipids are regarded as main future feedstock of biofuels for its higher efficiency of accumulation and sus- tainable production. In order to investigate the effect of various nitrogen to phosphorus ratios on cells growth, chlorophyll content and accumulation of lipids in Dunaliella tertiolecta, experiments were carried out in modified microalgal medium with inorganic nitrogen （nitrate-nitrogen） or organic nitrogen （urea-nitrogen） as the sole nitrogen source at initial N：P ratios ranging from 1：1 to 32：1. The favorable N：P of 16：1 in the nitrate-N or urea-N medium yielded the maximum cell density and specific growth rate. Decrease in chlorophyll content were observed at the N：P of 4：1 in both nitrate-N and urea-N cultures. It was also observed that the maximum lipids concentration was obtained at the N：P of 4：1 in both nitrate and urea nutrient medium. The lipid productivity and lipid content of cultures in the urea-N medium at the N：P of 4： lwere markedly higher than those from cultures with other N：P ratios （p〈 0.05）. The results of this work illustrate the possibility that higher ratios of nitrogen to phosphorus have enhancing effect on cells growth of D. tertiolecta. Conversely, higher lipid accumulation is associated with a decrease in chlorophyll content under lower ratios of nitro- gen to phosphorus. The results confirm the hypothesis of this study that a larger metabolic flux has been channeled to lipid accumu- lation in D. tertiolecta cells when the ratios of nitrogen to phosphorus drop below a critical level.展开更多
Age-related macular degeneration(AMD)is a progressive retinal disease,which is the leading cause of blindness in western countries.There is an urgency to establish new therapeutic strategies that could prevent or dela...Age-related macular degeneration(AMD)is a progressive retinal disease,which is the leading cause of blindness in western countries.There is an urgency to establish new therapeutic strategies that could prevent or delay the progression of AMD more efficiently.Until now,the pathogenesis of AMD has remained unclear,limiting the development of the novel therapy.Bruch’s membrane(BM)goes through remarkable changes in AMD,playing a significant role during the disease course.The main aim of this review is to present the crucial processes that occur at the level of BM,with special consideration of the lipid accumulation and protein modifications.Besides,some therapies targeted at these molecules and the construction of BM in tissue engineering of retinal pigment epithelium(RPE)cells transplantation were listed.Hopefully,this review may provide a reference for researchers engaged in pathogenesis or management on AMD.展开更多
The aim of this study was to explore the lipid-lowering effect of naringenin and the underlying mechanism in high-fat-diet-fed SD rats and 3T3-L1 cells.In this study,SD rats were divided into the normal chow diet grou...The aim of this study was to explore the lipid-lowering effect of naringenin and the underlying mechanism in high-fat-diet-fed SD rats and 3T3-L1 cells.In this study,SD rats were divided into the normal chow diet group(NCD),high fat diet group(HFD),three treatment groups feeding high-fat diet with naringenin(100,200,400 mg/kg)for 12 weeks.Results indicated that naringenin treatment decreased total cholesterol(TC),triglyceride(TG)and the non-high-density lipoprotein cholesterol(non-HDL-C)levels in serum.Naringenin also alleviated hepatic steatosis and reduced the adipocyte size in the epididymis in high-fat-diet-induced SD rats.In addition,naringenin(25−75μg/mL)decrease TG and TC levels in 3T3 mature adipocytes.The molecular mechanism of naringenin in the treatment of obesity were predicted by using network pharmacology.Real-time PCR analysis results showed that naringenin regulated the expression of lipid metabolism genes.Meanwhile,naringenin increased the AMPK(AMP-activated protein kinase)activity and the expression of AMPK phosphorylated protein in 3T3 mature adipocytes.And the inhibitory effect of naringenin on lipid accumulation in 3T3 adipocytes was abolished by Compound C.Molecular docking results indicated that naringenin could bind to AMPK protein.These results indicated naringenin reduced lipid accumulation through AMPK pathway.展开更多
The major pathologic hallmark of the alcoholic liver disease(ALD)is the representation of chronic alcohol-induced hepatocyte lipid accumulation.This study aims to investigate the hepatoprotective role of triterpenoids...The major pathologic hallmark of the alcoholic liver disease(ALD)is the representation of chronic alcohol-induced hepatocyte lipid accumulation.This study aims to investigate the hepatoprotective role of triterpenoids-enriched extracts from Antrodia cinnamomea mycelia(ACT)in chronic alcohol-induced liver injury mice,establishing in C57BL/6 mice through gradient alcohol feeding for 24 weeks.In longterm alcohol consumption mice,the significantly lost body weight,increased organ indexes,hepatic alanine aminotransferase and aspartate aminotransferase levels were all remissed after 6-week ACT orally administration,showing its hepatoprotective property.ACT suppressed the triglyceride,total cholesterol and low-density lipoprotein levels,and enhanced high-density lipoprotein levels in serum or/and liver of chronic alcohol damaged mice.Combining with the pathological observations,ACT displayed an anti-steatosis effects to restrain the progress of ALD.Based on proteomic analysis and enzyme-linked immunosorbent assay,ACT had been confi rmed to regulate the levels of lipid biogeneration-related factors and depressed the over-accumulation of hepatic reactive oxygen species.According to further data,ACT prevented alcoholic liver injury may be associated with mediating lipid metabolism-related to PGC-1αand NF-κB signaling.In summary,ACT protected the body against chronic alcohol ingest induced liver injury through its regulation lipid on metabolism.展开更多
Calanus sinicus,the dominant copepod in the Yellow Sea,develops a large oil sac in late spring to prepare for over-summering in the Yellow Sea Cold Water Mass(YSCWM).The lipid accumulation mechanism for the initiation...Calanus sinicus,the dominant copepod in the Yellow Sea,develops a large oil sac in late spring to prepare for over-summering in the Yellow Sea Cold Water Mass(YSCWM).The lipid accumulation mechanism for the initiation of over-summering is unknown.Here,we cultured C3 copepodites at four constant temperatures(10,13,16,and 19℃) and at three temperature regimes that mimicked the temperature variations experienced during diurnal vertical migration(10-13℃,10-16℃,and 10-19℃) for 18 days to explore the effects of temperature differences on copepod development and lipid accumulation.C.sinicus stored more lipid at low than at high temperatures.A diurnal temperature difference(10-16℃ and 10-19℃)promoted greater lipid accumulation(1.9-2.1 times) than a constant temperature of either 16℃ or 19℃,by reducing the energy cost at colder temperatures and lengthening copepodite development.Thereafter,the lipid reserve supported gonad development after final molting.Only one male developed in these experiments.This highly female-skewed sex ratio may have been the result of the monotonous microalgae diet fed to the copepodites.This study provides the first evidence that diurnal temperature differences may promote lipid accumulation in C.sinicus,and provides a foundation for future investigations into the mechanisms involved in over-summering in the YSCWM.展开更多
Low molecular weight polysaccharides can be isolated from Sargassum thunbergii(LMPST)and in vitro experiments were conducted to evaluate the inhibitory effects on lipids.Two natures of LMPST were attained from S.thunb...Low molecular weight polysaccharides can be isolated from Sargassum thunbergii(LMPST)and in vitro experiments were conducted to evaluate the inhibitory effects on lipids.Two natures of LMPST were attained from S.thunbergii and appraised their LMPST on palmitic acid(PA)induced lipid accretion in Hep G2,and 3T3-L1 cells.LMPST treatment lessened lipid deposition and intracellular free fatty acid and triglyceride intensities in PA-treated above mentioned cells.The mechanistic study publicized that LMPST2 significantly suppressed adipogenesis and stimulated the PA-treated 3T3-L1 cells occupied in the lipolysis pathway.Furthermore,in PA-treated Hep G2 cells,the free fatty acid oxidation was significantly increased by LMPST2.Given these constructive properties of LMPST2 from S.thunbergii,is a potential candidate for diminishing the intracellular lipids,and for a therapeutic agent in those conditions.展开更多
This study aims to investigate the impact of hepatocyte nuclear factor 1β(HNF1b)on macrophage sortilin-mediated lipid metabolism and aortic atherosclerosis and explore the role of the flavone of Polygonatum odoratum(...This study aims to investigate the impact of hepatocyte nuclear factor 1β(HNF1b)on macrophage sortilin-mediated lipid metabolism and aortic atherosclerosis and explore the role of the flavone of Polygonatum odoratum(PAOA-flavone)-promoted small ubiquitin-related modifier(SUMO)modification in the atheroprotective efficacy of HNF1b.HNF1b was predicted to be a transcriptional regulator of sortilin expression via bioinformatics,dual-luciferase reporter gene assay,and chromatin immunoprecipitation.HNF1b overexpression decreased sortilin expression and cellular lipid contents in THP-1 macrophages,leading to a depression in atherosclerotic plaque formation in low-density lipoprotein(LDL)receptor-deficient(LDLR−/−)mice.Multiple SUMO1-modified sites were identified on the HNF1b protein and co-immunoprecipitation confirmed its SUMO1 modification.The SUMOylation of HNF1b protein enhanced the HNF1b-inhibited effect on sortilin expression and reduced lipid contents in macrophages.PAOA-flavone treatment promoted SUMO-activating enzyme subunit 1(SAE1)expression and SAE1-catalyzed SUMOylation of the HNF1b protein,which prevented sortilin-mediated lipid accumulation in macrophages and the formation of atherosclerotic plaques in apolipoprotein E-deficient(ApoE−/−)mice.Interference with SAE1 abrogated the improvement in lipid metabolism in macrophage cells and atheroprotective efficacy in vivo upon PAOA-flavone administration.In summary,HNF1b transcriptionally suppressed sortilin expression and macrophage lipid accumulation to inhibit aortic lipid deposition and the development of atherosclerosis.This anti-atherosclerotic effect was enhanced by PAOA-flavone-facilitated,SAE1-catalyzed SUMOylation of the HNF1b protein.展开更多
Objective This study aimed to investigate the lipid-lowering activity of LFBEP-C1 in high glucose-fed Caenorhabditis elegans(C.elegans).Methods In this study,the fermented barley protein LFBEP-C1 was prepared and test...Objective This study aimed to investigate the lipid-lowering activity of LFBEP-C1 in high glucose-fed Caenorhabditis elegans(C.elegans).Methods In this study,the fermented barley protein LFBEP-C1 was prepared and tested for its potential anti-obesity effects on C.elegans.The worms were fed Escherichia coli OP50(E.coli OP50),glucose,and different concentrations of LFBEP-C1.Body size,lifespan,movement,triglyceride content,and gene expression were analyzed.The results were analyzed using ANOVA and Tukey's multiple comparison test.Results Compared with the model group,the head-swing frequency of C.elegans in the group of LFBEP-C1 at 20μg/mL increased by 33.88%,and the body-bending frequency increased by 27.09%.This indicated that LFBEP-C1 improved the locomotive ability of C.elegans.The average lifespan of C.elegans reached 13.55 days,and the body length and width of the C.elegans decreased after LFBEP-C1 intake.Additionally,LFBEP-C1 reduced the content of lipid accumulation and triglyceride levels.The expression levels of sbp-1,daf-2,and mdt-15 significantly decreased,while those of daf-16,tph-1,mod-1,and ser-4 significantly increased after LFBEP-C1 intake.Changes in these genes explain the signaling pathways that regulate lipid metabolism.Conclusion LFBEP-C1 significantly reduced lipid deposition in C.elegans fed a high-glucose diet and alleviated the adverse effects of a high-glucose diet on the development,lifespan,and exercise behavior of C.elegans.In addition,LFBEP-C1 regulated lipid metabolism mainly by mediating the expression of genes in the sterol regulatory element-binding protein,insulin,and 5-hydroxytryptamine signaling pathways.展开更多
Background:Nonalcoholic fatty liver disease(NAFLD)is a metabolic disorder of the liver with a complex underlying pathogenic mechanism characterized by excessive accumulation of lipids in liver cells,which can later le...Background:Nonalcoholic fatty liver disease(NAFLD)is a metabolic disorder of the liver with a complex underlying pathogenic mechanism characterized by excessive accumulation of lipids in liver cells,which can later lead to irreversible liver fibrosis.Dajianzhong decoction(DJZ)is a traditional Chinese medicine formula,and its medicinal components have the potential to treat metabolic diseases.This study aims to evaluate the effect of DJZ intervention on lipid accumulation in HepG2 cells in vitro.Methods:This study used a combination of network pharmacology and in vitro experimental validation to demonstrate the efficacy of DJZ in treating NAFLD using a commercial kit.And the key genes were characterized by PCR technology.Western blot was used to characterize the expression of core proteins in network pharmacology.Results:The results of network pharmacology indicate that DJZ shares 220 common targets with NAFLD.According to the MCOED plugin in PPI,the core targets include apoptosis regulator Bcl-2(Bcl2),apoptosis regulator BAX(BAX),Caspase-3(CASP3),mitogen-activated protein kinase 8(JNK),mitogen-activated protein kinase 1(MAPK1),peroxisome proliferator-activated receptor alpha(PPAR-α),etc.These targets also occupy important positions in the component target pathway disease network diagram,and were validated by molecular docking.In vitro experiments showed that DJZ reduced the intracellular total triglyceride(TG)and total cholesterol(TC)content of HepG2 cells,decreased the production of ROS in cells,and reduced apoptosis,playing a protective role in liver cells.The mRNA expression levels of some key fatty acid and cholesterol metabolism factors were also characterized by PCR.After administering DJZ to HepG2 cells,the mRNA levels of fatty acid synthesis proteins such as SREBP1 and FASN decreased,while the mRNA levels of fatty acid metabolism such as PPAR-αand ACOX1 increased.The core targets in network pharmacology were also validated by western blot,and the pathways involved were related to apoptosis and fatty acid oxidation.Conclusion:DJZ can effectively reduce the intracellular lipid levels and decrease cell apoptosis in HepG2 cells modeled with free fatty acids.Further research is needed in animal experiments and to improve the composition analysis of DJZ,in order to fully clarify the molecular mechanism and material basis of DJZ in treating NAFLD.展开更多
As significant differences in cellular physiology, metabolic potential and genetics occur among strains with morphological similarity, the screening of appropriate microalgae species for effective CO2 fixation and bio...As significant differences in cellular physiology, metabolic potential and genetics occur among strains with morphological similarity, the screening of appropriate microalgae species for effective CO2 fixation and biodiesel production is extremely critical. In this study, ten strains of Chlorella were cultivated in municipal wastewater influent（MWI） and their tolerance for MWI, CO2 fixation efficiency and lipid productivity were assessed. The results showed that the biomass concentrations of four strains（Chlorella vulgaris, Chlorella64.01, Chlorella regularis var. minima and Chlorella sp.） were significantly higher than other strains. When the cultivation systems were aerated with 10% CO2, Chlorella sp. showed the highest CO2 fixation efficiency（35.51%）, while the highest lipid accumulation（58.48%） was observed with C. vulgaris. Scanning electron microscopy images revealed that the cells of both Chlorella sp. and C. vulgaris kept their normal morphologies after 15 day batch culture.These findings indicated that Chlorella sp. and C. vulgaris have fairly good tolerance for MWI,and moreover, Chlorella sp. was appropriate for CO2 fixation while C. vulgaris represented the highest potential for producing biodiesel.展开更多
Lard,a fat rich in saturated fatty acids(SFAs),is regarded as a risk factor for metabolic diseases.In the present study,effect of different lard blended with sunflower oil diets on lipid accumulation in adipose tissue...Lard,a fat rich in saturated fatty acids(SFAs),is regarded as a risk factor for metabolic diseases.In the present study,effect of different lard blended with sunflower oil diets on lipid accumulation in adipose tissue,liver,and serum by mouse model was researched.Body weight,body fat percentage,cross-sectional area of adipocytes,liver triglycerides(TGs),and oil red stained area in mice liver of lard blend sunflower oil(L-SFO)group were significantly lower than those of sunflower oil(SFO)group,whereas no significant differences were observed between mice of lard and L-SFO groups.Serum TG and free fatty acid levels were significantly lower in L-SFO group than in other two groups.Furthermore,data showed that sunflower oil decreased contents of hormonesensitive lipase and carnitine palmitoyl transferase 1(CPT-1)and increased fatty acid synthase activity in liver tissue.A mixture of lard and sunflower oil rather than only sunflower oil or lard might promote body fat loss and reduce lipid accumulation in adipose tissue,serum,and liver by promoting hydrolysis of TG,increasingβ-oxidation of fatty acids.These data suggested that mixing lard and vegetable oil(e.g.sunflower oil)for cooking,or alternate using lard and vegetable oil could be beneficial for reducing body fat.展开更多
<span style="font-family:Verdana;">The Lipid Accumulation Product (LAP) is a clinical marker of visceral obesity and has been proposed as a simple, inexpensive, and accurate tool to estimate cardiovasc...<span style="font-family:Verdana;">The Lipid Accumulation Product (LAP) is a clinical marker of visceral obesity and has been proposed as a simple, inexpensive, and accurate tool to estimate cardiovascular risk and mortality. The aim of this study was to verify the association of LAP with anthropometric, biochemical, visceral adiposity index and IR in adults and the elderly. This single cross-section center clinical study, with experimental, analytical, primary, and observational design, included 210 participants. Anthropometric (Body Mass Index (BMI), Waist Circumference (WC), and Neck Circumference (NC)), LAP, Visceral Adipose Index (VAI), and biochemical parameters (fasting glycemia, insulinemia (to calculate the Homa-IR index), total cholesterol, LDL-c, HDL-c, and triglycerides) were evaluated. The results showed that by separating the sample into three groups (adequate BMI and WC, adequate BMI and elevated WC, and elevated BMI and WC), the group with high BMI and WC showed a high value of LAP and VAI compared to the other groups, with a significant difference. Still, the data show a positive and significant correlation when relating the LAP with VAI, HOMA-IR, BMI, WC, NC, total cholesterol, triglycerides, and Diastolic Blood Pressure. It also showed an inversely proportional relationship when associating LAP with HDL-c (p < 0.0001). Thus, we show that LAP is closely related to visceral adiposity, IR, altered lipid parameters, and blood pressure, especially diastolic in the patients included in our study. For these reasons, we suggest that LAP is a reliable indicator of promising visceral adiposity for early detection of cardiovascular risk in the adult and senior population.</span>展开更多
Objective To observe the efficacy of"Biaoben acupoint compatibility moxibustion for abdominal obesity and its effect on blood lipid,lipid accumulation product(LAP)and cardiometabolic index(CMI).Methods A total of...Objective To observe the efficacy of"Biaoben acupoint compatibility moxibustion for abdominal obesity and its effect on blood lipid,lipid accumulation product(LAP)and cardiometabolic index(CMI).Methods A total of 150 patients with abdominal obesity were randomly divided into an observation group(75cases,5 cases dropped out)and a control group(75cases,6 cases dropped out).展开更多
At the bend and bifurcation of arteries prone to atherosclerosis,pulsatile blood retention may cause overstretch on the tube wall.It has been reported that more than half of the foam cells found in atherosclerotic pla...At the bend and bifurcation of arteries prone to atherosclerosis,pulsatile blood retention may cause overstretch on the tube wall.It has been reported that more than half of the foam cells found in atherosclerotic plaques are derived from vascular smooth muscle cells(VSMCs),but the mechanism is not adequately understood.In this work,we used a microfluidic device to apply a cyclic stretch(15%and 0.05 Hz)on the VSMC for 24 h.The stretch caused a significant increase in the intracellular lipid accumulation,accompanying with the increased NOX1 and CD36 protein expression.On the other hand,inhibition of NOX1 activity,elimination of reactive oxygen species(ROS),or knockdown of NOX1 expression could significantly inhibit intracellular lipid accumulation.In addition,the NOX1 upregulation caused by 15%stretch was related to the JAK/STAT signaling pathway.Our results reveal a novel mechanism of VSMC foam cell formation caused by the upregulation of NOX1.展开更多
基金funded by Guangxi Science and Technology Major Project(Guike AA23023035,Guike AA22096020)the Joint Funds of the National Natural Science Foundation of China(U20A2004)+1 种基金Guilin Innovation Platform and Talent Plan(20210102-3)Guangxi Science and Technology Base and Talent Project(Guike AA21196009)。
文摘Siraitia grosvenorii(SG),a traditional edible medicine in China,has shown potential application in health foods due to multiple bioactivities.However,few comparative studies have investigated the effect of daily intake of SG products by traditional drinking way on alleviating metabolic disorders caused by long-term high fat and high sugar diet(HF-HSD).This study investigated the effects of 2 commercial SG products(SG juice containing comprehensive compositions and mogrosides extract containing high content of mogroside V)supplement on fat content,lipid accumulation,colon barrier and gut microbiota in a 38-week HF-HSD mice trial.Results showed that the SG intervention could decrease the weight gain and body fat content,alleviated lipid accumulation,low-grade inflammation and colon barrier injury.SG juice intake was found to increase the antioxidant enzyme activities of serum glutathione peroxidase and liver superoxide dismutase.16S r RNA analysis found that SG could restore the gut microbiota with downregulation of harmful bacteria increased by HF-HSD and enhancement of the abundances of beneficial genus.In particular,SG juice significantly enriched taxonomic family Prevotellaceae and genus Alloprevotella.These results indicated that SG might be a functional additive to prevent disease risk caused by excess fat and sugar diet,and active ingredients resulting to the discrepancy of performance especially on gut microbiota are worthy of in-depth investigation.
基金supported by the Basic Research Project of Yunnan Provincial Science and Technology Department(202201AT070262)the Project of Yunnan Province Food and Drug Homologous Resources Functional Food Innovation Team(A3032023057)+1 种基金the Yunnan Province Ten Thousand Plan Industrial Technology Talents Project(YNWR-CYJS-2020-010)the Yunnan Province-City Integration Project(202302 AN360002).
文摘Obesity has brought great challenges to global human health,and how to effectively prevent and control the occurrence and development of obesity has become an urgent problem.The role and mechanism of 4-[(α-Lrhamnosyloxy)benzyl]isothiocyanate(MITC),an active ingredient of Moringa oleifera Lam.,in the regulation of lipid metabolism have not been comprehensively investigated.In the present study,we investigated the mechanism of MITC in inhibiting lipid accumulation in mice fed with a high-fat diet(HFD)in terms of both lipolysis and central appetite regulation mediated by the gut microbe-gut-brain axis.MITC enhanced the characteristic indices associated with HFD mice and also promoted adipocytolysis and brown fat thermogenesis.Moreover,MITC was observed to improve leptin resistance,modulate the composition of gut microbiota such as Ruminococcaceae,Parasutterella,and Acetatifactor,promote 5-hydroxytryptamine secretion,further enhance the secretion of glucagon-like peptide-1(GLP-1)and peptide tyrosine-tyrosine(PYY)to activate peroxisome proliferator-activated receptor(PPAR)signaling in the hypothalamus,and modulate feeding behavior to inhibit lipid accumulation in HFD mice.These data suggest that MITC supplementation can help to alleviate obesity or obesity-related diseases.
基金This work was supported by the grants from National Natural Science Foundation of China(No.82174334)2022 Postgraduate Innovation Research Projects in Hainan Province(No.Qhys2022-273).
文摘Objective:To investigate the effects of Alpiniae oxyphyllae Fructus(AOF)on renal lipid deposition in diabetic kidney disease(DKD)and elucidate its molecular mechanisms.Methods:The mechanism of AOF in treating DKD was explored by network pharmacological enrichment analysis,molecular docking,and molecular dynamics simulation.The effects of AOF on renal function and lipid deposition were assessed in a mouse model of DKD and high glucose-stressed HK-2 cells.Cell viability and lipid accumulation were detected by CCK8 and oil red O staining.The expressions of PPARαand fatty acid oxidation-related genes(ACOX1 and CPT1A)were detected by quantitative RT-PCR,Western blot,and immunofluorescence.Furthermore,PPARαknockdown was performed to examine the molecular mechanism of AOF in treating DKD.Results:Network pharmacological enrichment analysis,molecular docking,and molecular dynamics simulation showed that the active compounds in AOF targeted PPARαand thus transcriptionally regulated ACOX1 and CPT1A.AOF lowered blood glucose,improved dyslipidemia,and attenuated renal injury in DKD mice.AOF-containing serum accentuated high glucose-induced decrease in cell viability and ameliorated lipid accumulation.Additionally,it significantly upregulated the expression of PPARα,ACOX1,and CPT1A in both in vivo and in vitro experiments,which was reversed by PPARαknockdown.Conclusions:AOF may promote fatty acid oxidation via PPARαto ameliorate renal lipid deposition in DKD.
基金Supported by Joint TCM Science&Technology Projects of National Demonstration Zones for Comprehensive TCM Reform,No.GZYKJS-ZJ-2025-044Ningbo Top Medical and Health Research Program,No.2023020612.
文摘BACKGROUND Metabolic dysfunction-associated steatotic liver disease(MASLD)has emerged as a prominent and pervasive global health challenge.Bicuculline(BIC),which is a key active component of the anti-MASLD prescription"Eight Zhes Decoction",has been preliminarily shown by our research team to have significant potential in treating MASLD.AIM To determine BIC's efficacy in treating MASLD by regulating lipid metabolism and suppressing hepatic inflammation via nuclear factor-kappa B(NF-κB)pathway,identifying it as a therapeutic candidate.METHODS This study explored the potential of BIC in preventing and treating MASLD using zebrafish,cellular(HepG2 and AML12),and mouse models.RESULTS Our results indicate that BIC significantly reduces lipid accumulation and inflammation both in vivo and in vitro.Transcriptomic analysis suggested that the anti-MASLD effects of BIC are linked to the inhibition of the NF-κB pathway,which plays a critical role in mitigating inflammation and lipid deposition.CONCLUSION This study is the first to demonstrate that BIC specifically alleviates lipid accumulation and hepatic steatosis in MASLD models via the NF-κB signaling pathway.Overall,BIC has emerged as a promising candidate for treating MASLD.
基金the National Natural Science Foundation of China(No.31900285)the Natural Science FoundationofAnhuiProvince(Nos.2108085QH309and 2208085MH203)Anhui Medical University"Three Complete Education"Comprehensive Reform Pilot Project(No.2021xsqyr05)。
文摘Alcoholic liver disease(ALD)is a growing global health concern,and its early pathogenesis includes steatosis and steatohepatitis.Inhibiting lipid accumulation and inflammation is a crucial step in relieving ALD.Evidence shows that puerarin(Pue),an isoflavone isolated from Pueraria lobata,exerts cardio-protective,neuroprotective,anti-inflammatory,antioxidant activities.However,the therapeutic potential of Pue on ALD remains unknown.In the study,both the NIAAA model and ethanol(EtOH)-induced AML-12 cell were used to explore the protective effect of Pue on alcoholic liver injury in vivo and in vitro and related mechanism.The results showed that Pue(100 mg·kg^(−1))attenuated EtOH-induced liver injury and inhibited the levels of SREBP-1c,TNF-α,IL-6 and IL-1β,compared with silymarin(Sil,100 mg·kg^(−1)).In vitro results were consistent with in vivo results.Mechanistically,Pue might suppress liver lipid accumulation and inflammation by regulating MMP8.In conclusion,Pue might be a promising clinical candidate for ALD treatment.
基金supported by the China Agriculture Research System (CARS-14)the National Natural Science Foundation of China (31000728,31100205)+2 种基金the Natural Science Fundation of Shangdong Province,China(ZR2009DQ004,ZR2011CQ036)the Promotive Research Fund for Young and Middle-Aged Scientisits of Shandong Province,China (BS2010NY023)the Qingdao Municipal Science and Technology Plan Project,China (11-2-4-9-(3)-jch,11-2-3-26-nsh)
文摘Phosphoenolpyruvate carboxylase（PEPC;EC 4.1.1.31） catalyses phosphoenolpyruvate（PEP） to yield oxaloacetate,which is involved in protein biosynthesis.Pyruvate kinase（PK;EC 2.7.1.40） catalyzes PEP to yield pyruvate,which is involved in fatty acid synthesis.In this study,five PEPC genes（AhPEPC1,AhPEPC2,AhPEPC3,AhPEPC4,and AhPEPC5） from peanut have been cloned.Using a quantitative real-time RT-PCR approach,the expression pattern of each gene was monitored during the seed development of four peanut varieties（E11,Hebeigaoyou,Naihan 1,and Huayu 26）.It was found that these five genes shared similar expression behaviors over the developmental stages of E11 with high expression levels at 30 and 40 d after pegging（DAP）;whereas these five genes showed irregular expression patterns during the seed development of Hebeigaoyou.In Naihan 1 and Huayu 26,the expression levels of the five genes remained relatively high in the first stage.The PEPC activity was monitored during the seed development of four peanut varieties and seed oil content was also characterized during whole period of seed development.The PEPC activity followed the oil accumulation pattern during the early stages of development but they showed a significantly negative correlation thereafter.These results suggested that PEPC may play an important role in lipid accumulation during the seed development of four peanut varieties tested.
基金partially supported by Tianjin Marine Revitalization Plan of Science and Technology (No.KJXH2013-16) from Tianjin Oceanic Administration,China
文摘Microalgal lipids are regarded as main future feedstock of biofuels for its higher efficiency of accumulation and sus- tainable production. In order to investigate the effect of various nitrogen to phosphorus ratios on cells growth, chlorophyll content and accumulation of lipids in Dunaliella tertiolecta, experiments were carried out in modified microalgal medium with inorganic nitrogen （nitrate-nitrogen） or organic nitrogen （urea-nitrogen） as the sole nitrogen source at initial N：P ratios ranging from 1：1 to 32：1. The favorable N：P of 16：1 in the nitrate-N or urea-N medium yielded the maximum cell density and specific growth rate. Decrease in chlorophyll content were observed at the N：P of 4：1 in both nitrate-N and urea-N cultures. It was also observed that the maximum lipids concentration was obtained at the N：P of 4：1 in both nitrate and urea nutrient medium. The lipid productivity and lipid content of cultures in the urea-N medium at the N：P of 4： lwere markedly higher than those from cultures with other N：P ratios （p〈 0.05）. The results of this work illustrate the possibility that higher ratios of nitrogen to phosphorus have enhancing effect on cells growth of D. tertiolecta. Conversely, higher lipid accumulation is associated with a decrease in chlorophyll content under lower ratios of nitro- gen to phosphorus. The results confirm the hypothesis of this study that a larger metabolic flux has been channeled to lipid accumu- lation in D. tertiolecta cells when the ratios of nitrogen to phosphorus drop below a critical level.
文摘Age-related macular degeneration(AMD)is a progressive retinal disease,which is the leading cause of blindness in western countries.There is an urgency to establish new therapeutic strategies that could prevent or delay the progression of AMD more efficiently.Until now,the pathogenesis of AMD has remained unclear,limiting the development of the novel therapy.Bruch’s membrane(BM)goes through remarkable changes in AMD,playing a significant role during the disease course.The main aim of this review is to present the crucial processes that occur at the level of BM,with special consideration of the lipid accumulation and protein modifications.Besides,some therapies targeted at these molecules and the construction of BM in tissue engineering of retinal pigment epithelium(RPE)cells transplantation were listed.Hopefully,this review may provide a reference for researchers engaged in pathogenesis or management on AMD.
文摘The aim of this study was to explore the lipid-lowering effect of naringenin and the underlying mechanism in high-fat-diet-fed SD rats and 3T3-L1 cells.In this study,SD rats were divided into the normal chow diet group(NCD),high fat diet group(HFD),three treatment groups feeding high-fat diet with naringenin(100,200,400 mg/kg)for 12 weeks.Results indicated that naringenin treatment decreased total cholesterol(TC),triglyceride(TG)and the non-high-density lipoprotein cholesterol(non-HDL-C)levels in serum.Naringenin also alleviated hepatic steatosis and reduced the adipocyte size in the epididymis in high-fat-diet-induced SD rats.In addition,naringenin(25−75μg/mL)decrease TG and TC levels in 3T3 mature adipocytes.The molecular mechanism of naringenin in the treatment of obesity were predicted by using network pharmacology.Real-time PCR analysis results showed that naringenin regulated the expression of lipid metabolism genes.Meanwhile,naringenin increased the AMPK(AMP-activated protein kinase)activity and the expression of AMPK phosphorylated protein in 3T3 mature adipocytes.And the inhibitory effect of naringenin on lipid accumulation in 3T3 adipocytes was abolished by Compound C.Molecular docking results indicated that naringenin could bind to AMPK protein.These results indicated naringenin reduced lipid accumulation through AMPK pathway.
基金This research was funded by the National Key Research&Development Program of China(grant number:2018YFE0107800)the Special Projects of the Cooperation between Jilin University and Jilin Province(grant number:SXGJXX2017-1)+1 种基金the Science and Technology Develop Project in Jilin Province of China under grant(No.20191102027YY,20200708091YY and 20200708068YY)Research and Cultivation Project for Young Teachers of Jiangxi Medical College,Nanchang University(No.PY201901).
文摘The major pathologic hallmark of the alcoholic liver disease(ALD)is the representation of chronic alcohol-induced hepatocyte lipid accumulation.This study aims to investigate the hepatoprotective role of triterpenoids-enriched extracts from Antrodia cinnamomea mycelia(ACT)in chronic alcohol-induced liver injury mice,establishing in C57BL/6 mice through gradient alcohol feeding for 24 weeks.In longterm alcohol consumption mice,the significantly lost body weight,increased organ indexes,hepatic alanine aminotransferase and aspartate aminotransferase levels were all remissed after 6-week ACT orally administration,showing its hepatoprotective property.ACT suppressed the triglyceride,total cholesterol and low-density lipoprotein levels,and enhanced high-density lipoprotein levels in serum or/and liver of chronic alcohol damaged mice.Combining with the pathological observations,ACT displayed an anti-steatosis effects to restrain the progress of ALD.Based on proteomic analysis and enzyme-linked immunosorbent assay,ACT had been confi rmed to regulate the levels of lipid biogeneration-related factors and depressed the over-accumulation of hepatic reactive oxygen species.According to further data,ACT prevented alcoholic liver injury may be associated with mediating lipid metabolism-related to PGC-1αand NF-κB signaling.In summary,ACT protected the body against chronic alcohol ingest induced liver injury through its regulation lipid on metabolism.
基金Supported by the State Key Program of National Natural Science Foundation of China(No.41230963)the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA11020305)the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences(No.U1406403)
文摘Calanus sinicus,the dominant copepod in the Yellow Sea,develops a large oil sac in late spring to prepare for over-summering in the Yellow Sea Cold Water Mass(YSCWM).The lipid accumulation mechanism for the initiation of over-summering is unknown.Here,we cultured C3 copepodites at four constant temperatures(10,13,16,and 19℃) and at three temperature regimes that mimicked the temperature variations experienced during diurnal vertical migration(10-13℃,10-16℃,and 10-19℃) for 18 days to explore the effects of temperature differences on copepod development and lipid accumulation.C.sinicus stored more lipid at low than at high temperatures.A diurnal temperature difference(10-16℃ and 10-19℃)promoted greater lipid accumulation(1.9-2.1 times) than a constant temperature of either 16℃ or 19℃,by reducing the energy cost at colder temperatures and lengthening copepodite development.Thereafter,the lipid reserve supported gonad development after final molting.Only one male developed in these experiments.This highly female-skewed sex ratio may have been the result of the monotonous microalgae diet fed to the copepodites.This study provides the first evidence that diurnal temperature differences may promote lipid accumulation in C.sinicus,and provides a foundation for future investigations into the mechanisms involved in over-summering in the YSCWM.
基金supported by Korea Institute of Marine Science&Technology Promotion(KIMST)funded by the Ministry of Oceans and Fisheries,Korea(20220488)。
文摘Low molecular weight polysaccharides can be isolated from Sargassum thunbergii(LMPST)and in vitro experiments were conducted to evaluate the inhibitory effects on lipids.Two natures of LMPST were attained from S.thunbergii and appraised their LMPST on palmitic acid(PA)induced lipid accretion in Hep G2,and 3T3-L1 cells.LMPST treatment lessened lipid deposition and intracellular free fatty acid and triglyceride intensities in PA-treated above mentioned cells.The mechanistic study publicized that LMPST2 significantly suppressed adipogenesis and stimulated the PA-treated 3T3-L1 cells occupied in the lipolysis pathway.Furthermore,in PA-treated Hep G2 cells,the free fatty acid oxidation was significantly increased by LMPST2.Given these constructive properties of LMPST2 from S.thunbergii,is a potential candidate for diminishing the intracellular lipids,and for a therapeutic agent in those conditions.
基金supported by the Natural Science Foundation of Guangxi Zhuang Autonomous Region,China (No.2019JJA140728)the Horizontal Cooperation Project with Hunan Mingshun Pharmaceutical Co.,Ltd. (No.2021GLHX02)the Guangxi Province Postgraduate Co-training Base for Cooperative Innovation in Basic Medicine of Guilin Medical University (No.Gui Xue Wei[2020]7).
文摘This study aims to investigate the impact of hepatocyte nuclear factor 1β(HNF1b)on macrophage sortilin-mediated lipid metabolism and aortic atherosclerosis and explore the role of the flavone of Polygonatum odoratum(PAOA-flavone)-promoted small ubiquitin-related modifier(SUMO)modification in the atheroprotective efficacy of HNF1b.HNF1b was predicted to be a transcriptional regulator of sortilin expression via bioinformatics,dual-luciferase reporter gene assay,and chromatin immunoprecipitation.HNF1b overexpression decreased sortilin expression and cellular lipid contents in THP-1 macrophages,leading to a depression in atherosclerotic plaque formation in low-density lipoprotein(LDL)receptor-deficient(LDLR−/−)mice.Multiple SUMO1-modified sites were identified on the HNF1b protein and co-immunoprecipitation confirmed its SUMO1 modification.The SUMOylation of HNF1b protein enhanced the HNF1b-inhibited effect on sortilin expression and reduced lipid contents in macrophages.PAOA-flavone treatment promoted SUMO-activating enzyme subunit 1(SAE1)expression and SAE1-catalyzed SUMOylation of the HNF1b protein,which prevented sortilin-mediated lipid accumulation in macrophages and the formation of atherosclerotic plaques in apolipoprotein E-deficient(ApoE−/−)mice.Interference with SAE1 abrogated the improvement in lipid metabolism in macrophage cells and atheroprotective efficacy in vivo upon PAOA-flavone administration.In summary,HNF1b transcriptionally suppressed sortilin expression and macrophage lipid accumulation to inhibit aortic lipid deposition and the development of atherosclerosis.This anti-atherosclerotic effect was enhanced by PAOA-flavone-facilitated,SAE1-catalyzed SUMOylation of the HNF1b protein.
基金supported by the priority academic program development of Jiangsu Higher education institutionsthe National Natural Science Foundation of China [31801538, 32072200]China Postdoctoral Science Foundation[2019M651747].
文摘Objective This study aimed to investigate the lipid-lowering activity of LFBEP-C1 in high glucose-fed Caenorhabditis elegans(C.elegans).Methods In this study,the fermented barley protein LFBEP-C1 was prepared and tested for its potential anti-obesity effects on C.elegans.The worms were fed Escherichia coli OP50(E.coli OP50),glucose,and different concentrations of LFBEP-C1.Body size,lifespan,movement,triglyceride content,and gene expression were analyzed.The results were analyzed using ANOVA and Tukey's multiple comparison test.Results Compared with the model group,the head-swing frequency of C.elegans in the group of LFBEP-C1 at 20μg/mL increased by 33.88%,and the body-bending frequency increased by 27.09%.This indicated that LFBEP-C1 improved the locomotive ability of C.elegans.The average lifespan of C.elegans reached 13.55 days,and the body length and width of the C.elegans decreased after LFBEP-C1 intake.Additionally,LFBEP-C1 reduced the content of lipid accumulation and triglyceride levels.The expression levels of sbp-1,daf-2,and mdt-15 significantly decreased,while those of daf-16,tph-1,mod-1,and ser-4 significantly increased after LFBEP-C1 intake.Changes in these genes explain the signaling pathways that regulate lipid metabolism.Conclusion LFBEP-C1 significantly reduced lipid deposition in C.elegans fed a high-glucose diet and alleviated the adverse effects of a high-glucose diet on the development,lifespan,and exercise behavior of C.elegans.In addition,LFBEP-C1 regulated lipid metabolism mainly by mediating the expression of genes in the sterol regulatory element-binding protein,insulin,and 5-hydroxytryptamine signaling pathways.
基金supported by the Joint Innovation Fund Project between the Third Affiliated Hospital of Chengdu University of Traditional Chinese Medicine and Chengdu Pidu Traditional Chinese Medicine Hospital(Grant No.LH202402041).
文摘Background:Nonalcoholic fatty liver disease(NAFLD)is a metabolic disorder of the liver with a complex underlying pathogenic mechanism characterized by excessive accumulation of lipids in liver cells,which can later lead to irreversible liver fibrosis.Dajianzhong decoction(DJZ)is a traditional Chinese medicine formula,and its medicinal components have the potential to treat metabolic diseases.This study aims to evaluate the effect of DJZ intervention on lipid accumulation in HepG2 cells in vitro.Methods:This study used a combination of network pharmacology and in vitro experimental validation to demonstrate the efficacy of DJZ in treating NAFLD using a commercial kit.And the key genes were characterized by PCR technology.Western blot was used to characterize the expression of core proteins in network pharmacology.Results:The results of network pharmacology indicate that DJZ shares 220 common targets with NAFLD.According to the MCOED plugin in PPI,the core targets include apoptosis regulator Bcl-2(Bcl2),apoptosis regulator BAX(BAX),Caspase-3(CASP3),mitogen-activated protein kinase 8(JNK),mitogen-activated protein kinase 1(MAPK1),peroxisome proliferator-activated receptor alpha(PPAR-α),etc.These targets also occupy important positions in the component target pathway disease network diagram,and were validated by molecular docking.In vitro experiments showed that DJZ reduced the intracellular total triglyceride(TG)and total cholesterol(TC)content of HepG2 cells,decreased the production of ROS in cells,and reduced apoptosis,playing a protective role in liver cells.The mRNA expression levels of some key fatty acid and cholesterol metabolism factors were also characterized by PCR.After administering DJZ to HepG2 cells,the mRNA levels of fatty acid synthesis proteins such as SREBP1 and FASN decreased,while the mRNA levels of fatty acid metabolism such as PPAR-αand ACOX1 increased.The core targets in network pharmacology were also validated by western blot,and the pathways involved were related to apoptosis and fatty acid oxidation.Conclusion:DJZ can effectively reduce the intracellular lipid levels and decrease cell apoptosis in HepG2 cells modeled with free fatty acids.Further research is needed in animal experiments and to improve the composition analysis of DJZ,in order to fully clarify the molecular mechanism and material basis of DJZ in treating NAFLD.
文摘As significant differences in cellular physiology, metabolic potential and genetics occur among strains with morphological similarity, the screening of appropriate microalgae species for effective CO2 fixation and biodiesel production is extremely critical. In this study, ten strains of Chlorella were cultivated in municipal wastewater influent（MWI） and their tolerance for MWI, CO2 fixation efficiency and lipid productivity were assessed. The results showed that the biomass concentrations of four strains（Chlorella vulgaris, Chlorella64.01, Chlorella regularis var. minima and Chlorella sp.） were significantly higher than other strains. When the cultivation systems were aerated with 10% CO2, Chlorella sp. showed the highest CO2 fixation efficiency（35.51%）, while the highest lipid accumulation（58.48%） was observed with C. vulgaris. Scanning electron microscopy images revealed that the cells of both Chlorella sp. and C. vulgaris kept their normal morphologies after 15 day batch culture.These findings indicated that Chlorella sp. and C. vulgaris have fairly good tolerance for MWI,and moreover, Chlorella sp. was appropriate for CO2 fixation while C. vulgaris represented the highest potential for producing biodiesel.
基金This research was funded by the National Key Research and Development Program of China(2016YDF0501200).
文摘Lard,a fat rich in saturated fatty acids(SFAs),is regarded as a risk factor for metabolic diseases.In the present study,effect of different lard blended with sunflower oil diets on lipid accumulation in adipose tissue,liver,and serum by mouse model was researched.Body weight,body fat percentage,cross-sectional area of adipocytes,liver triglycerides(TGs),and oil red stained area in mice liver of lard blend sunflower oil(L-SFO)group were significantly lower than those of sunflower oil(SFO)group,whereas no significant differences were observed between mice of lard and L-SFO groups.Serum TG and free fatty acid levels were significantly lower in L-SFO group than in other two groups.Furthermore,data showed that sunflower oil decreased contents of hormonesensitive lipase and carnitine palmitoyl transferase 1(CPT-1)and increased fatty acid synthase activity in liver tissue.A mixture of lard and sunflower oil rather than only sunflower oil or lard might promote body fat loss and reduce lipid accumulation in adipose tissue,serum,and liver by promoting hydrolysis of TG,increasingβ-oxidation of fatty acids.These data suggested that mixing lard and vegetable oil(e.g.sunflower oil)for cooking,or alternate using lard and vegetable oil could be beneficial for reducing body fat.
文摘<span style="font-family:Verdana;">The Lipid Accumulation Product (LAP) is a clinical marker of visceral obesity and has been proposed as a simple, inexpensive, and accurate tool to estimate cardiovascular risk and mortality. The aim of this study was to verify the association of LAP with anthropometric, biochemical, visceral adiposity index and IR in adults and the elderly. This single cross-section center clinical study, with experimental, analytical, primary, and observational design, included 210 participants. Anthropometric (Body Mass Index (BMI), Waist Circumference (WC), and Neck Circumference (NC)), LAP, Visceral Adipose Index (VAI), and biochemical parameters (fasting glycemia, insulinemia (to calculate the Homa-IR index), total cholesterol, LDL-c, HDL-c, and triglycerides) were evaluated. The results showed that by separating the sample into three groups (adequate BMI and WC, adequate BMI and elevated WC, and elevated BMI and WC), the group with high BMI and WC showed a high value of LAP and VAI compared to the other groups, with a significant difference. Still, the data show a positive and significant correlation when relating the LAP with VAI, HOMA-IR, BMI, WC, NC, total cholesterol, triglycerides, and Diastolic Blood Pressure. It also showed an inversely proportional relationship when associating LAP with HDL-c (p < 0.0001). Thus, we show that LAP is closely related to visceral adiposity, IR, altered lipid parameters, and blood pressure, especially diastolic in the patients included in our study. For these reasons, we suggest that LAP is a reliable indicator of promising visceral adiposity for early detection of cardiovascular risk in the adult and senior population.</span>
文摘Objective To observe the efficacy of"Biaoben acupoint compatibility moxibustion for abdominal obesity and its effect on blood lipid,lipid accumulation product(LAP)and cardiometabolic index(CMI).Methods A total of 150 patients with abdominal obesity were randomly divided into an observation group(75cases,5 cases dropped out)and a control group(75cases,6 cases dropped out).
基金supported by the National Natural Science Foundation of China(31571481,31771585,and 32070799).
文摘At the bend and bifurcation of arteries prone to atherosclerosis,pulsatile blood retention may cause overstretch on the tube wall.It has been reported that more than half of the foam cells found in atherosclerotic plaques are derived from vascular smooth muscle cells(VSMCs),but the mechanism is not adequately understood.In this work,we used a microfluidic device to apply a cyclic stretch(15%and 0.05 Hz)on the VSMC for 24 h.The stretch caused a significant increase in the intracellular lipid accumulation,accompanying with the increased NOX1 and CD36 protein expression.On the other hand,inhibition of NOX1 activity,elimination of reactive oxygen species(ROS),or knockdown of NOX1 expression could significantly inhibit intracellular lipid accumulation.In addition,the NOX1 upregulation caused by 15%stretch was related to the JAK/STAT signaling pathway.Our results reveal a novel mechanism of VSMC foam cell formation caused by the upregulation of NOX1.
