OBJECTIVE To investigate the effect of neuroprotective effect of lychee seed saponins(LSS) in BV-2.METHODS Aβ_(1-42) induced BV-2 cells were incubated with LSS for 12 h,the content of the inflammatory factors such as...OBJECTIVE To investigate the effect of neuroprotective effect of lychee seed saponins(LSS) in BV-2.METHODS Aβ_(1-42) induced BV-2 cells were incubated with LSS for 12 h,the content of the inflammatory factors such as IL^(-1)β,TNF-α,COX-2 and i NOS in the supernatant of BV-2 cell were measured by ELISA.The detection of the m RNA levels and the protein expression of the inflammatory factors including IL^(-1)β,TNF-α,COX-2 and i NOS using real-time PCR and Western blotting,respectively.RESULTS The level of IL^(-1)β,COX-2 and i NOS significantly increased with the treatment of Aβ_(1-42),and 0.117 mg·L^(-1)-0.469 mg·L^(-1) LSS can inhibit these increased level.CONCLUSION LSS conferred neuroprotection via inhibiting the inflammatory factors expression.展开更多
Long-duration manned submersible missions require advanced life support systems (LSS) that can regenerate air, water and food. This study presented two CO_2-capture methods used in LSS, CO_2 removal with diethanolamin...Long-duration manned submersible missions require advanced life support systems (LSS) that can regenerate air, water and food. This study presented two CO_2-capture methods used in LSS, CO_2 removal with diethanolamine (DEA) and cryo-freezing with liquid oxygen. Both processes were modeled and simulated with HYSYS simulator. The performance of the two types of module was compared, and the results showed that the latter could be advantageous over the former in specific power, facility scale, operation reliability and safety. Economic evaluation suggested the latter cost only half of the former. Cryo-capture module could be an alternative for underwater LSS because of its efficiency and compactness.展开更多
文摘OBJECTIVE To investigate the effect of neuroprotective effect of lychee seed saponins(LSS) in BV-2.METHODS Aβ_(1-42) induced BV-2 cells were incubated with LSS for 12 h,the content of the inflammatory factors such as IL^(-1)β,TNF-α,COX-2 and i NOS in the supernatant of BV-2 cell were measured by ELISA.The detection of the m RNA levels and the protein expression of the inflammatory factors including IL^(-1)β,TNF-α,COX-2 and i NOS using real-time PCR and Western blotting,respectively.RESULTS The level of IL^(-1)β,COX-2 and i NOS significantly increased with the treatment of Aβ_(1-42),and 0.117 mg·L^(-1)-0.469 mg·L^(-1) LSS can inhibit these increased level.CONCLUSION LSS conferred neuroprotection via inhibiting the inflammatory factors expression.
文摘Long-duration manned submersible missions require advanced life support systems (LSS) that can regenerate air, water and food. This study presented two CO_2-capture methods used in LSS, CO_2 removal with diethanolamine (DEA) and cryo-freezing with liquid oxygen. Both processes were modeled and simulated with HYSYS simulator. The performance of the two types of module was compared, and the results showed that the latter could be advantageous over the former in specific power, facility scale, operation reliability and safety. Economic evaluation suggested the latter cost only half of the former. Cryo-capture module could be an alternative for underwater LSS because of its efficiency and compactness.
