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A Study on the Form,Material,and Cultural Value of the Rosewood Four-Head Official’s Cap Chair With Golden Flecks:A Case Study of the Inscribed Piece by Wang Shixiang
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作者 HUANG Yun LIN Hai-tao 《Journal of Literature and Art Studies》 2026年第1期43-46,共4页
Ming-style furniture represents the pinnacle of Chinese traditional furniture,with the four-head official’s cap chair standing out as a representative category due to its classic form and cultural connotations.This p... Ming-style furniture represents the pinnacle of Chinese traditional furniture,with the four-head official’s cap chair standing out as a representative category due to its classic form and cultural connotations.This paper takes a specific rosewood four-head official’s cap chair inscribed by Wang Shixiang as the research subject,employing a tripartite approach of form analysis,material examination,and inscription interpretation.The chair strictly adheres to the classic form of Ming-style four-head official’s cap chairs,embodying the Ming Dynasty design philosophy of“the unity of function and aesthetics”.The scarcity and unique grain pattern of the golden-flecked rosewood(Zitan)endow it with the status of“gold among woods”.Wang Shixiang’s inscription binds the furniture to the cultural essence of“the charm of the Ming Dynasty”,transforming it into“heritable cultural heritage”.This paper provides a new perspective for the case study of Ming-style furniture and offers theoretical reference for the contemporary inheritance of traditional craftsmanship. 展开更多
关键词 Ming-style furniture Four-head official’s cap chair rosewood with golden flecks Wang shixiang inscription cultural value
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清燥救肺汤通过FOXK-FOXO-SKP2/ACSS2-TFEB-CARM1双通路调控肺癌细胞自噬溶酶体形成相关蛋白的表达
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作者 谢斌 田佳玄 +1 位作者 安欣 余功 《中药药理与临床》 北大核心 2026年第2期36-42,共7页
目的:检测自噬体形成(FOXK-FOXO-SKP2)与溶酶体合成(ACSS2-TFEB-CARM1)通路蛋白表达,结合透射电镜观察自噬溶酶体,探究清燥救肺汤抑制荷Lewis小鼠肺癌增殖机制。方法:以雄性C57BL/6J小鼠右腋皮下注射Lewis细胞建立动物模型,随机分为模... 目的:检测自噬体形成(FOXK-FOXO-SKP2)与溶酶体合成(ACSS2-TFEB-CARM1)通路蛋白表达,结合透射电镜观察自噬溶酶体,探究清燥救肺汤抑制荷Lewis小鼠肺癌增殖机制。方法:以雄性C57BL/6J小鼠右腋皮下注射Lewis细胞建立动物模型,随机分为模型对照组、环磷酰胺50 mg/kg组(腹腔注射,隔日1次)、清燥救肺汤2.7、5.5、11 g/kg组。各组造模前2 w给药,接种后连续给药2 w。试验结束后处死小鼠并取荷瘤组织,称瘤质量、瘤体积、计算体质量变化及瘤质比;透射电镜观察肺癌细胞自噬溶酶体的形成;qRT-PCR法检测肺癌组织Foxk1、Foxk2、Foxo3、Acss2、Skp2、Carm1 mRNA的表达;Western blot法检测肺癌组织p-TFEB-1蛋白表达;荧光显微镜下观察肺癌组织GFP-LC3蛋白表达。结果:与模型对照组比较,环磷酰胺组与清燥救肺汤2.7、5.5、11 g/kg组瘤体积及瘤质比显著降低、p-TFEB-1蛋白表达显著下调(P<0.01);环磷酰胺组和清燥救肺汤5.5、11 g/kg组GFP-LC3蛋白、Acss2 mRNA表达明显上调,Foxk2 mRNA表达明显下调(P<0.05或P<0.01);环磷酰胺组和清燥救肺汤11 g/kg组瘤质量显著降低(P<0.01),Foxk1、Foxk2、Skp2 mRNA表达下调,Foxo3、Carm1 mRNA表达明显上调(P<0.05或P<0.01);电镜下清燥救肺汤5.5、11 g/kg组可见自噬溶酶体。结论:清燥救肺汤可诱导肺癌细胞自噬,抑制肺癌增殖,减小瘤体积及瘤质比,其机制可能为通过上调GFP-LC3蛋白及Foxo3 mRNA的表达,下调Foxk1、Foxk2和Skp2的mRNA表达,促进自噬体的形成;下调p-TFEB-1蛋白表达、上调Acss2、Carm1 mRNA的表达促进溶酶体的形成,最终促进自噬融酶体的形成实现。 展开更多
关键词 清燥救肺汤 肺癌 自噬溶酶体 叉头框k亚家族转录因子-叉头框O亚家族转录因子-s期激酶相关蛋白2通路 乙酰辅酶A合成酶2-转录因子EB-共激活因子相关的精氨酸甲基转移酶1通路
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基于改进K-shell算法与多维价值融合的产业链关键共性技术识别研究——以半导体产业链为例
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作者 陈哲 李华 +1 位作者 吴爱萍 裴骁 《中国科技论坛》 北大核心 2026年第3期147-159,共13页
识别关键共性技术对提升产业链创新协同具有重要意义。针对现有研究仅关注单一技术价值或其简单组合的局限,提出一种融合技术属性与关系价值的识别方法。以半导体产业链为例,将半导体产业链产品、组成及工艺作为关键词来源检索专利,提取... 识别关键共性技术对提升产业链创新协同具有重要意义。针对现有研究仅关注单一技术价值或其简单组合的局限,提出一种融合技术属性与关系价值的识别方法。以半导体产业链为例,将半导体产业链产品、组成及工艺作为关键词来源检索专利,提取IPC小组号表征技术;基于普适性与关联性构建共性技术网络;引入随机森林算法构建评价体系评估各技术属性价值,设计改进后K-shell算法并在共性技术网络中创新性考虑邻域关联技术融合价值、间接关联技术融合价值影响力对各技术重要度排序,识别关键共性技术。识别出涉及半导体生长、刻蚀、薄膜沉积、先进成型及制造与封装测试技术等核心技术领域的24种关键共性技术,为产业链关键共性技术精准识别提供科学有效的方法支撑。 展开更多
关键词 产业链 关键共性技术 技术价值融合 k-shell算法
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利用Ripley’s K函数法的制造业微观企业数据空间集聚分析
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作者 赖冠中 《地理空间信息》 2026年第3期61-66,共6页
在制造业产业升级背景下,以广州市白云区现状建设用地和典型工业集聚区上的制造业微观企业为研究对象,进行多尺度和单双变量Ripley’s K函数空间集聚分析。结果表明:①在两种研究窗口范围下,纺织服装、服饰业为主的劳动密集型行业的集... 在制造业产业升级背景下,以广州市白云区现状建设用地和典型工业集聚区上的制造业微观企业为研究对象,进行多尺度和单双变量Ripley’s K函数空间集聚分析。结果表明:①在两种研究窗口范围下,纺织服装、服饰业为主的劳动密集型行业的集聚程度最高,契合白云区现状产业情况;②白云区大部分行业两两之间的吸引分布与行业劳动力共享和上下游产业链条有关;③对局部6片典型工业集聚区的分析表明,与全区总体分布情况类似,但木制家具业和技术密集型在6片区的集聚表现比全区更明显,人和镇东华工业集聚区形成明显的技术密集型行业集聚。 展开更多
关键词 制造业 工业集聚区 单双变量Ripley’s k函数 空间集聚 白云区
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不同危险度MDS患者PBMC中BCL-xL、HERV-K gag水平及对病情向急性白血病转换的预测价值
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作者 孙璨 杜姗姗 +2 位作者 郭敏 周静秋 章莉 《检验医学与临床》 2026年第2期230-237,共8页
目的研究不同危险度骨髓增生异常综合征(MDS)患者外周血单个核细胞(PBMC)中B淋巴细胞瘤-X基因长片段(BCL-xL)、人内源性逆转录病毒-K gag(HERV-K gag)水平及对MDS患者病情向急性髓系细胞白血病(AML)转换的预测价值。方法选取2020年1月至... 目的研究不同危险度骨髓增生异常综合征(MDS)患者外周血单个核细胞(PBMC)中B淋巴细胞瘤-X基因长片段(BCL-xL)、人内源性逆转录病毒-K gag(HERV-K gag)水平及对MDS患者病情向急性髓系细胞白血病(AML)转换的预测价值。方法选取2020年1月至2024年1月成都市第七人民医院和四川大学华西医院收治的218例MDS患者作为研究对象,随访1年,根据病情向AML转换情况,将其分为转换组、未转换组。收集MDS患者基线资料。使用倾向性评分匹配(PSM)降低组间偏倚。比较不同危险度(较低危、较高危)MDS患者及转换组和未转换组PBMC中BCL-xL mRNA、HERV-K gag mRNA水平。采用多因素Logistic回归分析MDS患者病情向AML转换的影响因素,根据危险度(较低危、较高危)分层,对回归分析结果进行敏感性检验。绘制受试者工作特征(ROC)曲线分析PBMC中BCL-xL mRNA、HERV-K gag mRNA单独及联合预测MDS患者病情向AML转换的价值。结果218例MDS患者中有1例失访,其余217例患者均获得随访,其中61例转换为AML(转换组),AML转换率为28.11%,另156例纳入未转换组。按照1∶1 PSM后共筛选出61例患者纳入转换组和61例患者纳入未转换组,2组各项基线资料比较,差异均无统计学意义(P>0.05)。较高危MDS患者PBMC中BCL-xL mRNA水平明显低于较低危患者,HERV-K gag mRNA水平明显高于较低危患者,差异均有统计学意义(P<0.05)。转换组患者PBMC中BCL-xL mRNA水平明显低于未转换组,HERV-K gag mRNA水平明显高于未转换组,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,PBMC中BCL-xL mRNA水平升高是MDS患者病情向AML转换的独立保护因素(P<0.05),HERV-K gag mRNA水平升高是MDS患者病情向AML转换的独立危险因素(P<0.05),且在较低危、较高危患者中,PBMC中BCL-xL mRNA水平升高仍是MDS患者病情向AML转换的独立保护因素(P<0.05),PBMC中HERV-K gag mRNA水平升高仍是MDS患者病情向AML转换的独立危险因素(P<0.05),提示研究结果较为稳定。ROC曲线分析结果显示,PBMC中BCL-xL mRNA、HERV-K gag mRNA单独及联合预测MDS患者病情向AML转换的曲线下面积(AUC)分别为0.754、0.762、0.874,二者联合预测的AUC明显大于PBMC中BCL-xL mRNA、HERV-K gag mRNA单独预测的AUC(Z=2.257,P=0.024;Z=2.246,P=0.025)。结论不同危险度MDS患者PBMC中BCL-xL mRNA、HERV-K gag mRNA水平存在明显差异,PBMC中BCL-xL mRNA、HERV-K gag mRNA水平与MDS患者病情向AML转换有关,且二者联合检测能提高对MDS患者病情向AML转换的预测能力,二者均可为临床随访管理和治疗决策提供重要的参考信息。 展开更多
关键词 危险度 骨髓增生异常综合征 外周血单个核细胞 B淋巴细胞瘤-X基因长片段 人内源性逆转录病毒-k gag基因 急性白血病 转换 预测价值
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Assessment of self- and cross-absorbed SAF values for HDRK-man using GEANT4 code: internal photon and electron emitters 被引量:1
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作者 Maroua Bhar Omrane Kadri Kais Manai 《Nuclear Science and Techniques》 SCIE CAS CSCD 2019年第10期40-47,共8页
The ultimate need to account for the partial amount of energy deposited in target tissue/organ resulting from internal inhalation,ingestion,and injection intakes of radionuclides,defined by the Medical Internal Radiat... The ultimate need to account for the partial amount of energy deposited in target tissue/organ resulting from internal inhalation,ingestion,and injection intakes of radionuclides,defined by the Medical Internal Radiation Dosimetry committee as the specific absorbed fraction(SAF),has become obvious.In this study,we assessed the SAF values for self-and cross-absorption,which were calculated for a uniform distribution of monoenergetic photon and electron emitters with energies ranging from 15 keV to 3 MeV.The voxelized human phantom‘‘High-Definition Reference Korean-man’’(HDRK-man),which was implemented using the Monte Carlo simulation code GEANT4(version 10.1),was used for several combinations of target–source organs.The results were compared to those of the International Commission on Radiological Protection Reference(ICRP133)and Zubal phantoms.It was found that the SAF values of the three models have a similar trend.However,the SAF values for the HDRK-man phantom were higher than those of the other two models,with a relatively good agreement with those for the ICRP133 phantom(differences of 13.9±2.8 and 12.1±3.2 for photon and electron emitters,respectively).To analyze the differences in SAF values,we calculated the chord length distributions(CLDs)for selected target–source combinations.The parameters of organ mass(or volume)and CLDs,in addition to the adopted computational procedures,mainly cause such discrepancies.For realistic radionuclide emission spectra,an overall overestimation was observed when computing the S values for three radiopharmaceuticals studied(I-131,In-111,and Lu-177)and for liver–spleen intra-and inter-organ absorption when compared with published data.The new arrangement of S and SAF values is expected to add value for multidisciplinary research and clinical communities. 展开更多
关键词 GEANT4 Monte Carlo simulation INTERNAL DOsIMETRY HDRk-man s and sAF values
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Values of Siva’s Constant “<i>K</i>” for All Fundamental Forces—A Review on Spin, Threshold Time and Quantum Entanglement 被引量:2
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作者 Siva Prasad Kodukula 《Journal of Modern Physics》 2019年第4期466-476,共11页
Siva’s constant “K” has been explained in brief. Its numerical values have been calculated for each fundamental force of nature. Spin of quantum mechanics has been interpreted in terms of Sivas constant “K”. Thus... Siva’s constant “K” has been explained in brief. Its numerical values have been calculated for each fundamental force of nature. Spin of quantum mechanics has been interpreted in terms of Sivas constant “K”. Thus limitation to velocity of light and interrelation between relativity and quantum mechanics has been explained in a novel and profound way. Involvement of “physics of consciousness” in synchronizing relativity and quantum mechanics has been emphasized. Concept of “bio force” as fifth fundamental force in addition to other four fundamental forces, strong, weak, electromagnetic and gravitational forces also has been emphasized. Consciousness has been explained as entanglement between bio force particle named as “jeeton” and gravitational force particle “graviton”. Thus frequency mediated consciousness has been explained. 展开更多
关键词 siva’s CONsTANT k Bio Force Particle Jeeton sPIN Grand Unification QUANTUM Mechanics Relativity QUANTUM Entanglement
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Comparison of K_(IC) Values for SiC Whisker Reinforced Ceramic Composites Obtained by Using Various Methods
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作者 Tingquan LEI Guangyong LIN +1 位作者 Shuangxi WANG and Yu ZHOU (Dept. of Metals and Technology, Harbin Institute of Technology, Harbin, 150001, China)(To whom correspondence should be addressed) 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 1994年第5期331-340,共10页
The fracture toughness (KIC) values determined by indentation microfracture method (IM ) for SiC whisker reinforced Al2O3 and ZrO2 based composites were calculated with different IM equations and compared with those o... The fracture toughness (KIC) values determined by indentation microfracture method (IM ) for SiC whisker reinforced Al2O3 and ZrO2 based composites were calculated with different IM equations and compared with those obtained by singte edge notched beam (SENB) technique. Experimental results show that the KIC (IM) values calculated with different equations are quite different one from another. For composites without phase transformable components the KIC (IM) and KIC (SENB) values are practically on the same level, but for composites with phase transformable components (partially stabilized zirconia) the KIC (SENB) values are always higher than KIC (IM). This is because that the IM method can not reveal sensitively the toughening effect due to dynamic t-m transformation of ZrO2 as the SENB method does. The accuracy of the IM method depends on the Suitability of the IM equations and was evaluated for the materials used in this investigation. Two new IM equations are suggested with which the KIC (IM ) values can be obtained very close to KIC (SENB) values for composites having phase transformable components. 展开更多
关键词 siC values for siC Whisker Reinforced Ceramic Composites Obtained by Using Various Methods Comparison of k IC
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Kincaid's Cultural Values in The Bridges of Madison County
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作者 LIU Xi WANG Xiao-yan 《Journal of Literature and Art Studies》 2014年第7期509-512,共4页
Robert James Waller (1939-2014) does not catch readers' attention until his popular novel The Bridges of Madison County (1996) wins him great admirations since its publication in 1992 which brings him instant suc... Robert James Waller (1939-2014) does not catch readers' attention until his popular novel The Bridges of Madison County (1996) wins him great admirations since its publication in 1992 which brings him instant success. The story explores the dilemma between responsibility of family and fulfillment of desires. Set in America in the 1960s, the novel is woven by the romantic story of the two middle-aged man and woman after they countered and gathered together for four days. Though simple in plot, it really deserves detailed interpretation and appreciation. This paper will mainly discuss Kincaid's cultural values reflected not only in the romance between his lover and him, but also in his attitudes towards women's rights. 展开更多
关键词 cultural values self-establishment woman's rights the other
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The Winter's Tale by Shakespeare and the Confucian Values
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作者 QIAN Zhi-fu HAN Li-li 《Journal of Literature and Art Studies》 2013年第6期333-343,共11页
Through comparison, this paper finds out that William Shakespeare (1564-1616), the most popular and wide respected writer in all English literatures and a great dramatist and humanist in the English Renaissance peri... Through comparison, this paper finds out that William Shakespeare (1564-1616), the most popular and wide respected writer in all English literatures and a great dramatist and humanist in the English Renaissance perio ly d, coincides in speaking highly of love, loyalty, intelligence, harmony, integrity, righteousness, friendship, and so on with Chinese Confucian values--the most precious treasure of Chinese traditional culture reflected in the Three Cardinal Guides and the Five Constant Virtues used by Chinese people in maintaining the stability and harmony of the whole nation and society--through vivid interpretation of human ethical relations in many of his works. At present, the world comparative literature studies have entered the third stage in the circumstance of globalization and internationalization, this finding is with paramount significance in construction of the mechanism of mutual recognition, mutual justification, mutual supplementation and mutual appreciation of heterogeneous cultures. This study, taking The Winter's Tale (1623) as a case, is about universality, unity, and shared values of the Confucianism and Shakespeare's plays in the perspective of the coexistence of multiple cultures. This paper is with four parts: (1) mechanism of mutual understanding, mutual justification, mutual supplementation, and mutual appreciation introduced in the first part; (2) the Confucian values addressed in details in the second part; (3) the third part is a case study; and (4) the last part shows how the Chinese and Western literatures and cultures can be understood, assisted, communicated, and appreciated with each other by the way of comparing the great works of Shakespeare and the Confucian values featured with universalism to a certain extent 展开更多
关键词 "four-mutuals the Confucian values Three Cardinal Guides and Five Constant Virtues TheWinter's Tale
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丹寿汤调控mTOR/S6K通路对人绒毛膜滋养层细胞HTR-8/Svneo自噬及增殖、凋亡的影响 被引量:1
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作者 于喜乐 卫爱武 +2 位作者 石少琦 崔天薇 宋红艳 《华中科技大学学报(医学版)》 北大核心 2025年第1期45-51,共7页
目的探讨丹寿汤调控mTOR/S6K通路对人绒毛膜滋养层细胞HTR-8/Svneo自噬及增殖、凋亡的影响。方法将HTR-8/Svneo分为对照组、丹寿汤低(丹寿汤-L,100μmol/mL)、中(丹寿汤-M,150μmol/mL)、高剂量(丹寿汤-H,200μmol/mL)组、mTOR通路激活... 目的探讨丹寿汤调控mTOR/S6K通路对人绒毛膜滋养层细胞HTR-8/Svneo自噬及增殖、凋亡的影响。方法将HTR-8/Svneo分为对照组、丹寿汤低(丹寿汤-L,100μmol/mL)、中(丹寿汤-M,150μmol/mL)、高剂量(丹寿汤-H,200μmol/mL)组、mTOR通路激活剂MHY1485(100 nmol/mL)组、丹寿汤-H+MHY1485组,处理24 h后,检测细胞增殖、凋亡、侵袭与迁移,观察自噬小体变化,检测LC3、p62、Beclin1 mRNA表达及mTOR/S6K通路相关蛋白的表达。结果与对照组相比,不同剂量的丹寿汤组增殖率、侵袭、迁移率、自噬小体/细胞质总面积、LC3、Beclin1 mRNA、p-mTOR/mTOR、S6K蛋白表达显著降低,凋亡率、p62 mRNA表达增加,组间差异有统计学意义,但MHY1485组增殖率、侵袭、迁移率、自噬小体/细胞质总面积、LC3、Beclin1 mRNA、p-mTOR/mTOR、S6K蛋白表达显著增加,凋亡率、p62 mRNA表达降低(均P<0.05);与丹寿汤-H组相比,丹寿汤-H+MHY1485组增殖率、侵袭、迁移率、自噬小体/细胞质总面积、LC3、Beclin1 mRNA、p-mTOR/mTOR、S6K蛋白表达显著增加,凋亡率、p62 mRNA表达降低(均P<0.05)。结论丹寿汤调控mTOR/S6K通路抑制人绒毛膜滋养层细胞HTR-8/Svneo自噬、增殖,诱导其凋亡。 展开更多
关键词 丹寿汤 mTOR/s6k通路 人绒毛膜滋养层细胞 自噬
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Pearl S. Buck’s Values of “Cultural Convergence” and Their Application
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作者 WANG Yan ZHU Ze-yuan SHI Hui-ge 《Cultural and Religious Studies》 2023年第1期9-18,共10页
The typical features of internal and external integration of cultures as revealed by the conception of“cultural convergence”provide a new perspective on the study of cultural exchanges and mutual learning.In the con... The typical features of internal and external integration of cultures as revealed by the conception of“cultural convergence”provide a new perspective on the study of cultural exchanges and mutual learning.In the context of“a global community of shared future for humankind”,this paper adopts the novel idea of“cultural convergence”to probe the growth of Pearl S.Buck’s cultural values and the specific contents and representation of Pearl S.Buck’s conception of“cultural convergence”,highlighting the application of Pearl S.Buck’s values to cultural education,growth and success,family education,cultural exchanges,humanistic practices,etc.Feasible suggestions and countermeasures are put forward to better promote the cultural exchanges and mutual learning as well as the convergence of different cultures. 展开更多
关键词 Pearl s.Buck values of“cultural convergence” applied research
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Preliminary <i>k</i>-Values of Unbound Natural Quartzitic Gravels for Mechanistic-Empirical Pavement Design
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作者 Edmund Obeng Yaw A. Tuffour +1 位作者 Daniel A. Obeng Bernard Koranteng-Yorke 《Open Journal of Civil Engineering》 2017年第4期509-526,共18页
The generalized constitutive model relating the resilient modulus (MR) of flexible pavement layer materials to stress state, adopted by the Mechanistic-Empirical Pavement Design Guide (MEPDG), contains a set of consta... The generalized constitutive model relating the resilient modulus (MR) of flexible pavement layer materials to stress state, adopted by the Mechanistic-Empirical Pavement Design Guide (MEPDG), contains a set of constants known as k-values (k1, k2, and k3) which are associated with the physical state of the layer materials. In Ghana, natural gravels constitute the predominant and sometimes the sole layer materials for most flexible pavements yet representative k-values of gravel materials, have not been determined to permit full application and implementation of the mechanistic-empirical design concept to pavements involving such materials. In this study, k-values characterising typical natural quartzitic gravels used for road construction in the country were derived by regression techniques from MR values determined using laboratory repeated load triaxial test. Using multiple linear regression technique, correlation relationships were then developed between the k-values and the physical properties of the gravels, namely, percentages of materials passing the 9.5 mm (P9.5) and 2.0 mm (P2.0) sieves, liquid limit (LL), maximum dry density (ρdmax), and optimum moisture content (wopt). The regression analysis returned k1 values which ranged between 441 and 958 with a mean of 516;k2 which varied between 0.0636 and 0.2168 with a mean value of 0.1216;and, k3 values which ranged between 0.1257 and 3.1590 with a mean value of 1.723. Contrary to what is mostly reported in literature, the analysis returned positive k3 values for all but one gravel material, suggesting stress hardening under octahedral shear stress for those materials. While an expanded sample base is required to fully characterize the whole gamut of natural gravels used in pavement construction in the country, this study on limited quartzitic gravel samples has given a good indication of strong linear correlations between the k-values and the index properties of the gravels, to permit estimates of the constants for such gravels be made where capability and opportunity for conducting resilient modulus tests do not exist.However, further work is recommended to fully characterise the exact nature of k3 values for quartzitic gravels in the country. 展开更多
关键词 Flexible Pavement Index Properties k-values Mechanistic-Empirical Design Quartzitic GRAVEL Resilient Modulus
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温肾健脾方通过p70S6K信号通路调控自噬减轻糖尿病肾病大鼠足细胞损伤的机制
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作者 史波 李如瑶 +2 位作者 金汀龙 王金 曹晓丹 《中国药理学通报》 北大核心 2025年第3期567-573,共7页
目的探究基于70 ku核糖体S6激酶(phosphoprotein 70 S6 kinase,p70S6K)信号通路的温肾健脾方调控自噬减轻糖尿病肾病(diabetic nephropathy,DN)大鼠足细胞损伤的作用机制。方法将链脲佐菌素诱导的DN模型大鼠分为5组,每组6只,即DN对照组... 目的探究基于70 ku核糖体S6激酶(phosphoprotein 70 S6 kinase,p70S6K)信号通路的温肾健脾方调控自噬减轻糖尿病肾病(diabetic nephropathy,DN)大鼠足细胞损伤的作用机制。方法将链脲佐菌素诱导的DN模型大鼠分为5组,每组6只,即DN对照组、温肾健脾方低剂量组(7.5 g·kg^(-1)·d^(-1))、温肾健脾方中剂量组(15 g·kg^(-1)·d^(-1))、温肾健脾方高剂量组(30 g·kg^(-1)·d^(-1))、缬沙坦阳性对照组(25 mg·kg^(-1)·d^(-1)),另以6只正常大鼠作为阴性对照组(等渗NaCl溶液10 mL·kg^(-1)·d^(-1)),各组大鼠连续灌胃8周。测定各组大鼠空腹血糖、尿液白蛋白/肌酐比值(urine albumin/creatinine ratio,UACR)和血液黏滞性,透射电镜观察各组大鼠足细胞结构和自噬小体变化,Western blot检测各组大鼠肾脏组织信号通路因子p70S6K和自噬因子p62的表达水平,免疫组化检测各组大鼠肾脏组织p62的表达水平。结果温肾健脾方可降低DN大鼠空腹血糖和UACR比值,改善血液黏滞性。透射电镜提示,温肾健脾方能够明显改善DN模型大鼠足细胞结构,提高足细胞自噬水平。Western blot结果提示,DN模型大鼠肾脏细胞信号通路因子p70S6K和自噬因子p62表达水平比空白对照组大鼠明显升高,差异有统计学意义( P <0.01)。与模型对照组相比,不同浓度温肾健脾方干预后p70S6K和p62表达水平均有所下降( P <0.05),其中温肾健脾方中剂量组变化最为显著。免疫组化结果显示,与对照组大鼠相比,DN大鼠肾脏组织自噬因子p62水平升高,温肾健脾方对DN大鼠p62的表达有一定的抑制作用。 结论 温肾健脾方可能通过抑制DN大鼠肾脏组织p70S6K水平,降低自噬因子p62表达,诱导肾脏细胞自噬增强进而恢复细胞稳态。 展开更多
关键词 温肾健脾方 糖尿病肾病 自噬 足细胞 P70s6k P62
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S100A4通过PI3K/AKT/mTOR信号通路调控BCG感染的THP-1巨噬细胞自噬
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作者 李梦媛 刘悦阳 +3 位作者 聂雪伊 马伯利 杨易 徐金瑞 《中国病理生理杂志》 北大核心 2025年第12期2348-2355,共8页
目的:探究牛结核分枝杆菌减毒株卡介苗(bacillus Calmette-Guérin,BCG)感染人单核-巨噬细胞THP-1后,S100钙结合蛋白A4(S100 calcium-binding protein A4,S100A4)通过磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase,PI3K)/蛋白激... 目的:探究牛结核分枝杆菌减毒株卡介苗(bacillus Calmette-Guérin,BCG)感染人单核-巨噬细胞THP-1后,S100钙结合蛋白A4(S100 calcium-binding protein A4,S100A4)通过磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase,PI3K)/蛋白激酶B(protein kinase B,PKB/AKT)/哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)信号通路对BCG诱导的巨噬细胞自噬的调控作用。方法:采用S100A4小干扰RNA(S100A4 small interfering RNA,si-S100A4)转染或PI3K抑制剂LY294002预处理THP-1巨噬细胞,并经BCG感染,设置不同处理组和对照组,通过Western blot方法检测PI3K/AKT/mTOR信号通路相关蛋白和自噬相关蛋白[微管相关蛋白1轻链3-II(microtubule-associated protein 1 light chain 3-II,LC3-II)、自噬相关蛋白7(autophagy-related protein 7,Atg7)和beclin-1]的表达,mRFP-GFP-LC3自噬双标腺病毒方法检测自噬流,透射电子显微镜观察细胞中自噬体和自噬溶酶体数量变化。结果:与未感染组相比,BCG感染组中p-PI3K、p-AKT和p-mTOR蛋白水平显著降低(P<0.05)。与BCG感染组相比,si-S100A4+BCG组上述磷酸化蛋白水平显著升高(P<0.05),LC3-II、Atg7和beclin-1蛋白水平显著降低(P<0.01);LY294003+BCG组上述磷酸化蛋白水平显著降低(P<0.01),LC3-II、Atg7和beclin-1蛋白水平显著升高(P<0.05),自噬体和自噬溶酶体数量显著增多(P<0.01)。与si-S100A4+BCG组相比,si-S100A4+LY294002+BCG组中p-PI3K、p-AKT和p-mTOR蛋白水平显著降低(P<0.01),LC3-II、Atg7和beclin-1蛋白水平显著升高(P<0.01),自噬体和自噬溶酶体数量显著增多(P<0.01),胞内菌载量显著减少(P<0.01)。结论:干扰S100A4经由PI3K/AKT/mTOR信号通路对BCG诱导的巨噬细胞自噬具有抑制作用。 展开更多
关键词 s100钙结合蛋白A4 结核分枝杆菌 巨噬细胞 自噬 PI3k/AkT/mTOR信号通路
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CEEMD小波包多阈值联合消噪后和变换时窗PAI⁃K/S⁃AIC微震初至波拾取方法研究 被引量:1
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作者 胡刚 张楚旋 +3 位作者 李夕兵 黄德龙 董陇军 蒲成志 《防灾减灾工程学报》 北大核心 2025年第3期542-553,共12页
重点分析研究了不同时窗长度对固定时窗PAI‑K/S拾取法拾取结果的影响及规律,提出了变换时窗在高阶统计量拾取法中的应用,进而采用变换时窗PAI‑K/S‑AIC联合法拾取波至时间。基于国内用沙坝矿的现场监测数据,首先采用CEEMD小波包多阈值联... 重点分析研究了不同时窗长度对固定时窗PAI‑K/S拾取法拾取结果的影响及规律,提出了变换时窗在高阶统计量拾取法中的应用,进而采用变换时窗PAI‑K/S‑AIC联合法拾取波至时间。基于国内用沙坝矿的现场监测数据,首先采用CEEMD小波包多阈值联合消噪法对微震数据进行消噪。对消噪后的微震信号,使用变换时窗PAI‑K/S‑AIC联合法拾取P波初至时间并与其他拾取方法进行横向对比分析。使用变换时窗PAI‑K/S‑AIC联合法拾取波至时间,避免人为选取STA/LTA阈值和时窗长度对拾取结果的影响,同时也解决了阈值和时窗的选取适用性较差的问题。变换时窗PAI‑K‑AIC联合法对消噪的微震信号拾取误差(≤20 ms)的事件数占90.95%(PAI‑S‑AIC为93%)。研究结果表明变换时窗PAI‑K/S‑AIC方法具有高效性、拾取精度高、适用范围广的特点。 展开更多
关键词 微震信号消噪 P波拾取 互补经验模态分解CEEMD 小波包多阈值 最小信息量准则 高阶统计量法PAI‑k/s
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Beta混合模型结合K-S检验的系统谐波阻抗估计 被引量:1
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作者 陈一涵 曾成碧 +1 位作者 苗虹 杨小宝 《电力系统及其自动化学报》 北大核心 2025年第6期121-128,共8页
为提高概率分布类方法在系统谐波阻抗估计中的准确性和稳健性,提出Beta混合模型结合柯尔莫可洛夫-斯米洛夫(Kolmogorov-Smirnov,K-S)检验的系统谐波阻抗估计方法。首先,基于电力系统等效电路构建系统谐波电流的Beta混合模型,根据最大似... 为提高概率分布类方法在系统谐波阻抗估计中的准确性和稳健性,提出Beta混合模型结合柯尔莫可洛夫-斯米洛夫(Kolmogorov-Smirnov,K-S)检验的系统谐波阻抗估计方法。首先,基于电力系统等效电路构建系统谐波电流的Beta混合模型,根据最大似然估计原理建立模型的对数似然函数。其次,采用期望最大算法进行参数估计,通过求解对数似然函数,实现系统谐波阻抗的准确估计。最后,引入K-S检验方法,根据谐波电流数据的实际累积分布和理论累积分布计算检验统计量,检验Beta混合模型的系统谐波电流分布模拟能力。在仿真测试和实例分析中与多种方法进行对比,结果表明本文所提方法能够提高系统谐波阻抗估计的准确性和稳健性。 展开更多
关键词 电能质量 谐波阻抗估计 Beta混合模型 最大似然估计 期望最大算法 柯尔莫可洛夫-斯米洛夫检验
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(n,k)-排列图的t/s诊断度与t/s诊断算法研究
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作者 张世豪 冷明 《计算机科学》 北大核心 2025年第S1期893-901,共9页
鉴于多处理器系统中日益严峻的故障风险挑战,特别是在超级计算机领域,如何有效提升系统的可靠性和容错能力成为了亟待解决的关键问题。(n,k)-排列图作为一种新型的互连网络拓扑结构应运而生,它是基于星图网络的推广和变形。它在保留星... 鉴于多处理器系统中日益严峻的故障风险挑战,特别是在超级计算机领域,如何有效提升系统的可靠性和容错能力成为了亟待解决的关键问题。(n,k)-排列图作为一种新型的互连网络拓扑结构应运而生,它是基于星图网络的推广和变形。它在保留星图网络原有的对称性和容错性的同时,具有更好的灵活性。目前对于(n,k)-排列图的可靠性研究尚不全面。基于此,展开了对(n,k)-排列图的t/s和t/s诊断算法研究。首先,给出了(n,k)-排列图的系列拓扑性质;然后,度量了(n,k)-排列图在PMC(Preparata,Metze,Chien)模型下的t/s诊断度;最后,设计了一个时间复杂度为O(N log2N)的快速诊断算法,用于识别(n,k)-排列图的所有故障结点。(n,k)-排列图的t/s诊断度被确定,进一步完善了(n,k)-排列图网络的可靠性指标,为其在应用和推广中的可靠性提供了重要的依据。 展开更多
关键词 可靠性 t/s诊断度 t/s诊断算法 (n k)-排列图 PMC模型
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联合稳健S变换与K均值聚类的高崩溃污染率拟稳点选取策略
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作者 刘忠贺 李宗春 +2 位作者 何华 郭迎钢 赵文斌 《测绘学报》 北大核心 2025年第9期1608-1619,共12页
合理选取拟稳点是变形监测网稳定性分析的关键一环。变形点数量较多甚至过半情况下,现有稳定性分析方法在选取拟稳点时稳健性不足,导致拟稳点选取结果不尽合理。为提高拟稳点选取的准确率,提出了一种联合稳健S变换模型与K均值聚类算法... 合理选取拟稳点是变形监测网稳定性分析的关键一环。变形点数量较多甚至过半情况下,现有稳定性分析方法在选取拟稳点时稳健性不足,导致拟稳点选取结果不尽合理。为提高拟稳点选取的准确率,提出了一种联合稳健S变换模型与K均值聚类算法的高崩溃污染率选取策略。首先,将两期变形监测网的同名点作为采样集合,从集合中随机选取部分点作为子集,利用稳健S变换模型求解子集中控制点的点位转换残差。然后,依据点位转换残差,采用K均值聚类算法将控制点分为拟稳点、小变形点及大变形点,并通过拟稳点集与大变形点集的点位转换残差质心差别判断稳健S变换模型是否可行,若稳健S变换模型奏效,则将转换残差小的点标记为拟稳点,从中选取频次较高的点作为备选拟稳点。最后,使用稳健S变换模型处理备选拟稳点以获得可靠的点位转换残差,以此为据,用K均值聚类算法准确选定拟稳点。通过仿真试验和实例分析,本文方法与传统相似变换模型、迭代加权相似变换模型、结合RANSAC算法的相似变换模型及平方型M_(split)相似变换模型进行了比较。结果表明,当变形监测网中存在变形点时,本文方法的变形点判别准确率最高,所得控制点位移更加符合实际。在变形点数量过半时,本文方法仍能保持稳健性,具有更高的崩溃污染率。 展开更多
关键词 变形监测网 稳健s变换 k均值聚类 稳定性分析 拟稳点
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基于AKT/S6K1信号通路探讨白术内酯Ⅰ抑制结直肠癌的作用机制
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作者 田薇 闫秋莹 +5 位作者 罗婧雯 吴其标 沈卫星 程海波 徐长亮 孙东东 《南京中医药大学学报》 北大核心 2025年第8期1037-1046,共10页
目的探讨白术内酯Ⅰ抑制结直肠癌的药效及作用机制。方法采用MTT实验从白术的三种主要活性成分中筛选出药效最好的白术内酯Ⅰ并筛选合理给药浓度。克隆形成实验检测白术内酯Ⅰ对LoVo细胞增殖的影响,流式细胞术检测其对LoVo细胞凋亡和周... 目的探讨白术内酯Ⅰ抑制结直肠癌的药效及作用机制。方法采用MTT实验从白术的三种主要活性成分中筛选出药效最好的白术内酯Ⅰ并筛选合理给药浓度。克隆形成实验检测白术内酯Ⅰ对LoVo细胞增殖的影响,流式细胞术检测其对LoVo细胞凋亡和周期的影响,伤口愈合及Transwell实验验证其对LoVo细胞迁移、侵袭的影响,Western blot检测其对LoVo细胞的增殖、迁移、EMT蛋白的影响。构建CT26小鼠皮下瘤模型,HE染色进行组织病理学分析,Western blot检测小鼠组织EMT蛋白的表达。结果白术内酯Ⅰ能抑制LoVo细胞增殖,且给药组与空白对照组相比有统计学差异(P<0.05,P<0.01)。白术内酯Ⅰ诱导LoVo细胞凋亡,给药组与空白对照组有差异(P<0.05,P<0.01)。白术内酯Ⅰ是通过G2/M期阻滞发挥抗肿瘤作用,与空白对照组相比,LoVo给药组的G2期细胞数增多,且有统计学差异(P<0.05)。细胞划痕和Transwell实验都能表明白术内酯Ⅰ能明显抑制肿瘤细胞的迁移和侵袭,LoVo给药组与对照组相比有统计学差异(P<0.05,P<0.01)。Western blot结果证明白术内酯Ⅰ能调控增殖蛋白c-Myc和凋亡蛋白Bcl-2的表达(P<0.05),下调周期蛋白CDK1、Cyclin B1、Cyclin D1及血管生成蛋白VEGF、MMP9(P<0.05),调控EMT蛋白E-Cadherin、N-Cadherin(P<0.05),并下调p-AKT、p-S6K1蛋白(P<0.05)。结论白术内酯Ⅰ可能通过AKT-S6K1信号通路发挥抗结直肠癌作用。 展开更多
关键词 白术内酯Ⅰ 结直肠癌 AkT/s6k1信号通路 上皮间质转化 LOVO细胞 CT26小鼠
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