[目的]探究OR13C2在宫颈癌组织中的表达以及对宫颈癌恶性转移的影响。[方法]通过蛋白免疫印迹和实时的荧光定量PCR检测宫颈癌组织OR13C2表达情况。将宫颈癌细胞分为pcDNA NC组、pcDNA OR13C2组和抑制剂(PI-103)组。CCK-8实验分析宫颈癌...[目的]探究OR13C2在宫颈癌组织中的表达以及对宫颈癌恶性转移的影响。[方法]通过蛋白免疫印迹和实时的荧光定量PCR检测宫颈癌组织OR13C2表达情况。将宫颈癌细胞分为pcDNA NC组、pcDNA OR13C2组和抑制剂(PI-103)组。CCK-8实验分析宫颈癌细胞增殖能力,Transwell实验和划痕实验分析宫颈癌细胞侵袭和迁移能力,流式细胞术检测宫颈癌细胞的凋亡率,蛋白免疫印迹分析OR13C2对PI3K/AKT通路蛋白表达的影响。[结果]OR13C2在宫颈癌组织中低表达,而在宫颈癌旁组织中高表达(0.23±0.02 vs 0.97±0.07;2.01±0.05 vs 4.81±0.13;P<0.05)。OR13C2高表达后能够抑制宫颈癌细胞的增殖、侵袭和迁移能力,促进宫颈癌细胞凋亡。此外,OR13C2高表达能够抑制PI3K/AKT的激活(0.73±0.03 vs 0.19±0.05 vs 0.26±0.08;0.72±0.08 vs 0.23±0.06 vs 0.31±0.03;P<0.05)。[结论]OR13C2在宫颈癌组织中表达减少,上调OR13C2的表达可以抑制宫颈癌HeLa细胞的增殖、侵袭和迁移能力,促进宫颈癌HeLa细胞凋亡,此作用与OR13C2抑制PI3K/AKT通路的激活相关。展开更多
In this article,we are concerned with the C^(2)estimates for the k-convex solutions of a class of degenerate k-Hessian equations on closed Hermitian manifolds,whose function in the right-hand side is relevant to the u...In this article,we are concerned with the C^(2)estimates for the k-convex solutions of a class of degenerate k-Hessian equations on closed Hermitian manifolds,whose function in the right-hand side is relevant to the unknown function and its gradient.We will get C^(0)estimate by promoting others′results,and get the“HMW estimate”of this equation such that the conditions of using blow-up analysis are satisfied,and the gradient estimate and second-order estimate will be obtained.Such an estimate will be helpful to study the existence for the solution of the equation.展开更多
文摘[目的]探究OR13C2在宫颈癌组织中的表达以及对宫颈癌恶性转移的影响。[方法]通过蛋白免疫印迹和实时的荧光定量PCR检测宫颈癌组织OR13C2表达情况。将宫颈癌细胞分为pcDNA NC组、pcDNA OR13C2组和抑制剂(PI-103)组。CCK-8实验分析宫颈癌细胞增殖能力,Transwell实验和划痕实验分析宫颈癌细胞侵袭和迁移能力,流式细胞术检测宫颈癌细胞的凋亡率,蛋白免疫印迹分析OR13C2对PI3K/AKT通路蛋白表达的影响。[结果]OR13C2在宫颈癌组织中低表达,而在宫颈癌旁组织中高表达(0.23±0.02 vs 0.97±0.07;2.01±0.05 vs 4.81±0.13;P<0.05)。OR13C2高表达后能够抑制宫颈癌细胞的增殖、侵袭和迁移能力,促进宫颈癌细胞凋亡。此外,OR13C2高表达能够抑制PI3K/AKT的激活(0.73±0.03 vs 0.19±0.05 vs 0.26±0.08;0.72±0.08 vs 0.23±0.06 vs 0.31±0.03;P<0.05)。[结论]OR13C2在宫颈癌组织中表达减少,上调OR13C2的表达可以抑制宫颈癌HeLa细胞的增殖、侵袭和迁移能力,促进宫颈癌HeLa细胞凋亡,此作用与OR13C2抑制PI3K/AKT通路的激活相关。
文摘In this article,we are concerned with the C^(2)estimates for the k-convex solutions of a class of degenerate k-Hessian equations on closed Hermitian manifolds,whose function in the right-hand side is relevant to the unknown function and its gradient.We will get C^(0)estimate by promoting others′results,and get the“HMW estimate”of this equation such that the conditions of using blow-up analysis are satisfied,and the gradient estimate and second-order estimate will be obtained.Such an estimate will be helpful to study the existence for the solution of the equation.
