BACKGROUND Nutritional and inflammatory indicators are crucial in assessing the nutritional health and immune function of patients with cancer,which are factors closely associated with the diagnosis and treatment of c...BACKGROUND Nutritional and inflammatory indicators are crucial in assessing the nutritional health and immune function of patients with cancer,which are factors closely associated with the diagnosis and treatment of colorectal cancer(CRC).AIM To explore the relationship between nutritional and inflammatory indicators and microsatellite stability(MSS)status in CRC.METHODS The clinical data of 56 patients who underwent surgical treatment for CRC were collected.Furthermore,the expressions of nutritional(levels of serum albumin,triglycerides,serum cholesterol,and body mass index)and inflammatory response indicators(absolute neutrophil count,absolute lymphocyte count,absolute monocyte count,neutrophil-to-lymphocyte ratio,and lymphocyte-to-monocyte ratio)as well as their correlation with microsatellite instability(MSI)status were investigated in patients with CRC.RESULTS Compared to the patients with MSS tumors,those with MSI tumors demonstrated significantly lower levels of two nutritional indicators,namely serum albumin and body mass index(P<0.05).Moreover,patients in the MSI group demonstrated significantly lower absolute lymphocyte counts and higher neutrophil-to-lymphocyte ratio than those in the MSS group(P<0.05),indicating pronounced differences in inflammatory responses and immune states between the two groups.CONCLUSION Certain nutritional and inflammatory indicators exhibit significant differences among patients with MSI and MSS CRC,highlighting their potential role in the clinical treatment and health management of this specific population.展开更多
The retrospective cohort study by Zuo et al investigates the interplay between microsatellite instability(MSI)status,nutritional indicators,and inflammatory profiles in colorectal cancer(CRC).Analyzing 56 patients,the...The retrospective cohort study by Zuo et al investigates the interplay between microsatellite instability(MSI)status,nutritional indicators,and inflammatory profiles in colorectal cancer(CRC).Analyzing 56 patients,the study reveals that MSI-high tumors are associated with significantly lower serum albumin,body mass index,and absolute lymphocyte counts,alongside elevated neutrophil-tolymphocyte ratios compared to microsatellite stable tumors.These findings highlight distinct immunological and nutritional profiles in MSI-high CRC,suggesting potential clinical utility in risk stratification and personalized treat-ment.While the study underscores the importance of MSI status in CRC mana-gement,its single-center design and limited sample size warrant validation through multicenter trials.This article contextualizes these findings within the broader landscape of CRC research,emphasizing the need for integrating bio-markerdriven strategies into clinical practice to optimize outcomes for patients with differing MSI statuses.展开更多
Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An ...Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An operando dual lossy mode resonance(LMR)biosensor fabricated on a D-shaped single-mode fiber(SMF)is proposed for quantification of clinical indicators of inflammatory process,like in COVID-19 infection.Dual LMRs,created via two-step deposition process,yield a nanostructure with distinct SnO_(2) thicknesses on the flat surface of the fiber.Theoretical and experimental analyses confirm its feasibility,showing a sensitivity around 4500 nm/RIU for both LMRs.A novel insight in spatially-separated biofunctionalization of the sensitive fiber regions is validated through fluorescence assays,showcasing selectivity for different immunoglobulins.Real-time and label-free detection of two inflammatory markers,C-reactive protein and Ddimer,empowers the platform capability with a minimum detectable concentration below 1μg/mL for both biomolecules,which is of clinical interest.This proof-of-concept work provides an important leap in fiber-based biosensing for effective and reliable multi-analyte detection,presenting a novel,compact and multi-functional analytical tool.展开更多
BACKGROUND Inflammation-related markers including neutrophil-to-lymphocyte ratio(NLR),platelet-to-lymphocyte ratio(PLR),monocyte-to-lymphocyte ratio(MLR),systemic immune-inflammation index(SII),systemic inflammation r...BACKGROUND Inflammation-related markers including neutrophil-to-lymphocyte ratio(NLR),platelet-to-lymphocyte ratio(PLR),monocyte-to-lymphocyte ratio(MLR),systemic immune-inflammation index(SII),systemic inflammation response index(SIRI)and prognostic nutritional index(PNI)could reflect tumor immune microenvironment and predict prognosis of cancers.However,it had not been explored in alpha-fetoprotein(AFP)producing gastric cancer(GC).AIM To determine the predictive value of inflammation-related peripheral blood markers including as NLR,PLR,MLR,SII,SIRI and PNI in the prognosis of AFPproducing GC(AFPGC).Besides,this study would also compare the differences in tumor immune microenvironment,clinical characteristics and prognosis between AFPGC and AFP-GC patients to improve the understanding of this disease.METHODS 573 patients enrolled were retrospectively studied.They were divided into AFP+group(AFP≥20 ng/mL)and AFP-group(AFP<20 ng/mL),comparing the levels of NLR/PLR/MLR/SII/SIRI/PNI and prognosis.In AFP+group,the impact of NLR/PLR/MLR/SII/SIRI/PNI and their dynamic changes on prognosis were further explored.RESULTS Compared with AFP-patients,AFP+patients had higher NLR/PLR/MLR/SII/-SIRI and lower PNI levels and poorer overall survival(OS).In the AFP+group,mortality was significantly lower in the lower NLR/PLR/MLR/SII/SIRI group and higher PNI group.Moreover,the dynamic increase(NLR/PLR/MLR/SII/-SIRI)or decrease(PNI)was associated with the rise of mortality within 1 year of follow-up.CONCLUSION Compared with AFP-patients,the level of inflammation-related peripheral blood markers significantly increased in AFP+patients,which was correlated with OS of AFP+patients.Also,the gradual increase of SII and SIRI was associated with the risk of death within one year in AFP+patients.AFPGC should be considered as a separate type and distinguished from AFP-GC because of the difference in tumor immune microenvironment.It requires basic experiments and large clinical samples in the future.展开更多
Objective:To observe the early interventions of traditional Chinese Medicine(TCM)on the conversion time of nucleic acid in patients with coronavirus disease 2019(COVID-19),and find possible underlying mechanisms of ac...Objective:To observe the early interventions of traditional Chinese Medicine(TCM)on the conversion time of nucleic acid in patients with coronavirus disease 2019(COVID-19),and find possible underlying mechanisms of action.Methods:A retrospective cohort study was conducted on 300 confirmed COVID-19 patients who were treated with TCM,at a designated hospital in China.The patients were categorized into three groups:TCM1,TCM2 and TCM3,who respectively received TCM interventions within 7,8–14,and greater than15 days of hospitalization.Different indicators such as the conversion time of pharyngeal swab nucleic acid,the conversion time of fecal nucleic acid,length of hospital stay,and inflammatory markers(leukocyte count,and lymphocyte count and percentage)were analyzed to observe the impact of early TCM interventions on these groups.Results:The median conversion times of pharyngeal swab nucleic acid in the three groups were 5.5,7 and16 d(P<0.001),with TCM1 and TCM2 being statistically different from TCM3(P<0.01).TCM1(P<0.05)and TCM3(P<0.01)were statistically different from TCM2.The median conversion times of fecal nucleic acid in the three groups were 7,9 and 17 d(P<0.001).Conversion times of fecal nucleic acid in TCM1 were statistically different from TCM3 and TCM2(P<0.01).The median lengths of hospital stay in the three groups were 13,16 and 21 d(P<0.001).TCM1 and TCM2 were statistically different from TCM3(P<0.01);TCM1 and TCM3 were statistically different from TCM2(P<0.01).Both leucocyte and lymphocyte counts increased gradually with an increase in the length of hospital stay in TCM1 group patients,with a statistically significant difference observed at each time point in the group(P<0.001).Statistically significant differences in lymphocyte count and percentage in TCM2(P<0.001),and in leucocyte count(P=0.043)and lymphocyte count(P=0.038)in TCM3 were observed.The comparison among the three groups showed a statistically significant difference in lymphocyte percentage on the third day of admission(P=0.044).Conclusion:In this study,it was observed that in COVID-19 patients treated with a combination of Chinese and Western medicines,TCM intervention earlier in the hospital stay correlated with faster conversion time of pharyngeal swab and fecal nucleic acid,as well as shorter length of hospital stay,thus helping promote faster recovery of the patient.The underlying mechanism of action may be related to improving inflammation in patients with COVID-19.展开更多
Objective To analyze the occurrence of metabolic dysfunction-associated fatty liver disease(MAFLD)and related inflammatory indicators in obstructive sleep apnea hypopnea syndrome(OSAHS)and explore the risk factors of ...Objective To analyze the occurrence of metabolic dysfunction-associated fatty liver disease(MAFLD)and related inflammatory indicators in obstructive sleep apnea hypopnea syndrome(OSAHS)and explore the risk factors of MAFLD.Methods This is a cross-sectional study.From January 2022 to October 2024,172 patients with sleep disorders were enrolled in the First Affiliated Hospital of Soochow University,including 38 patients with non-OSAHS,53 patients with mild OSAHS,37 patients with moderate OSAHS,and 44 patients with severe OSAHS.The occurrence of MAFLD was comprehensively judged from three aspects:metabolic dysfunction-associated fatty liver(MAFL),elevated liver enzymes,and liver fibrosis.The situation of MAFLD and the level of related inflammatory markers were compared among the four groups.Binary logistic regression was used to analyze the risk factors for MAFLD in OSAHS.Results There were significant differences in the prevalence of MAFL,the percentage of elevated liver enzymes,and interleukin-6 and tumor necrosis factoralpha levels among the four groups(P<0.05).The differences of fibrosis-4 index and C-reactive protein among the four groups were not statistically significant(P>0.05).Binary logistic regression showed that BMI,triglycerides,longest time of sleep apnea and tumor necrosis factor-alpha were the risk factors for MAFL(P<0.05).BMI,glucose,and apnea-hypopnea index were the risk factors for elevated liver enzymes(P<0.05).Conclusion OSAHS is strongly associated with MAFLD,and the involvement of OSAHS in the occurrence and development of MAFLD may be related to obesity,lipid metabolism disorders,insulin resistance,inflammatory responses,and intermittent hypoxia.展开更多
Aim:It remains unclear what the best therapeutic option for recurrent glioma patients after Stupp treatment is.Bevacizumab(BVZ)is commonly administered in progression,but it appears that only some patients benefit.It ...Aim:It remains unclear what the best therapeutic option for recurrent glioma patients after Stupp treatment is.Bevacizumab(BVZ)is commonly administered in progression,but it appears that only some patients benefit.It would be useful to find biomarkers that determine beforehand who these patients are.Methods:The protocol included 31 high-risk progressing glioma patients after Stupp treatment who received BVZ 5-10 mg/kg every 14 days and temozolomide(3-19 cycles,150-200 mg five days each 28-day cycle)during a mean of eight cycles of BVZ or until tumor progression or unacceptable toxicity.We analyzed the clinical outcome values of inflammatory indices measured before BVZ administration.Results:Lymphocyte level before BVZ administration was the best independent predictor of overall survival(HR=0.34;95%CI:0.145-0.81;P=0.015).The area under the receiver operating characteristic(ROC)curve was 0.823,with 1.645 being the optimal cut-off value,and 0.80 and 0.85 the sensitivity and specificity values,respectively.Responder and non-responder survival curves were also significantly different,considering the first and second tertiles as cut-off points.The number of BVZ cycles was not related to lymphopenia.Pretreatment neutrophil platelet levels,platelet-to-lymphocyte ratio(PLR),and neutrophil-to-lymphocyte ratio(NLR)did not have independent predictive value.Inflammatory variables were not correlated with each other.However,patients with high NLR and PLR simultaneously(double positive PLR-NLR)showed a worse clinical outcome than the rest(P=0.043).Conclusion:Pretreatment lymphocyte levels and double positive PLR-NLR could be used as non-invasive hematological prognostic markers for recurrent gliomas treated with bevacizumab.A close relationship emerged between inflammation and angiogenesis.展开更多
基金Supported by Grants of the Suzhou Medical Key Discipline,No.SZXK202109Suzhou Clinical Key Disease Project,No.LCZX202111Suzhou Promoting Health through Science and Education Research Project,No.KJXW2021028.
文摘BACKGROUND Nutritional and inflammatory indicators are crucial in assessing the nutritional health and immune function of patients with cancer,which are factors closely associated with the diagnosis and treatment of colorectal cancer(CRC).AIM To explore the relationship between nutritional and inflammatory indicators and microsatellite stability(MSS)status in CRC.METHODS The clinical data of 56 patients who underwent surgical treatment for CRC were collected.Furthermore,the expressions of nutritional(levels of serum albumin,triglycerides,serum cholesterol,and body mass index)and inflammatory response indicators(absolute neutrophil count,absolute lymphocyte count,absolute monocyte count,neutrophil-to-lymphocyte ratio,and lymphocyte-to-monocyte ratio)as well as their correlation with microsatellite instability(MSI)status were investigated in patients with CRC.RESULTS Compared to the patients with MSS tumors,those with MSI tumors demonstrated significantly lower levels of two nutritional indicators,namely serum albumin and body mass index(P<0.05).Moreover,patients in the MSI group demonstrated significantly lower absolute lymphocyte counts and higher neutrophil-to-lymphocyte ratio than those in the MSS group(P<0.05),indicating pronounced differences in inflammatory responses and immune states between the two groups.CONCLUSION Certain nutritional and inflammatory indicators exhibit significant differences among patients with MSI and MSS CRC,highlighting their potential role in the clinical treatment and health management of this specific population.
文摘The retrospective cohort study by Zuo et al investigates the interplay between microsatellite instability(MSI)status,nutritional indicators,and inflammatory profiles in colorectal cancer(CRC).Analyzing 56 patients,the study reveals that MSI-high tumors are associated with significantly lower serum albumin,body mass index,and absolute lymphocyte counts,alongside elevated neutrophil-tolymphocyte ratios compared to microsatellite stable tumors.These findings highlight distinct immunological and nutritional profiles in MSI-high CRC,suggesting potential clinical utility in risk stratification and personalized treat-ment.While the study underscores the importance of MSI status in CRC mana-gement,its single-center design and limited sample size warrant validation through multicenter trials.This article contextualizes these findings within the broader landscape of CRC research,emphasizing the need for integrating bio-markerdriven strategies into clinical practice to optimize outcomes for patients with differing MSI statuses.
基金financial support from the Spanish Agencia Estatal de Investigación (AEI) through project PID2023-149895OB-I00a predoctoral research grant from the Public University of Navarrafinancial support under the National Recovery and Resilience Plan (NRRP),Mission 4,Component 2,Investment 1.1,Call for tender No.1409 published on 14.9.2022 by the Italian Ministry of University and Research (MUR),funded by the European Union–NextGenerationEU–Project Title‘‘Fiber optics sensors as a platform for cancer diagnosis and in vitro model testing”–CUP B53D23024170001-Grant Assignment Decree No.1383 adopted on 01/09/2023 by the Italian MUR.
文摘Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An operando dual lossy mode resonance(LMR)biosensor fabricated on a D-shaped single-mode fiber(SMF)is proposed for quantification of clinical indicators of inflammatory process,like in COVID-19 infection.Dual LMRs,created via two-step deposition process,yield a nanostructure with distinct SnO_(2) thicknesses on the flat surface of the fiber.Theoretical and experimental analyses confirm its feasibility,showing a sensitivity around 4500 nm/RIU for both LMRs.A novel insight in spatially-separated biofunctionalization of the sensitive fiber regions is validated through fluorescence assays,showcasing selectivity for different immunoglobulins.Real-time and label-free detection of two inflammatory markers,C-reactive protein and Ddimer,empowers the platform capability with a minimum detectable concentration below 1μg/mL for both biomolecules,which is of clinical interest.This proof-of-concept work provides an important leap in fiber-based biosensing for effective and reliable multi-analyte detection,presenting a novel,compact and multi-functional analytical tool.
文摘BACKGROUND Inflammation-related markers including neutrophil-to-lymphocyte ratio(NLR),platelet-to-lymphocyte ratio(PLR),monocyte-to-lymphocyte ratio(MLR),systemic immune-inflammation index(SII),systemic inflammation response index(SIRI)and prognostic nutritional index(PNI)could reflect tumor immune microenvironment and predict prognosis of cancers.However,it had not been explored in alpha-fetoprotein(AFP)producing gastric cancer(GC).AIM To determine the predictive value of inflammation-related peripheral blood markers including as NLR,PLR,MLR,SII,SIRI and PNI in the prognosis of AFPproducing GC(AFPGC).Besides,this study would also compare the differences in tumor immune microenvironment,clinical characteristics and prognosis between AFPGC and AFP-GC patients to improve the understanding of this disease.METHODS 573 patients enrolled were retrospectively studied.They were divided into AFP+group(AFP≥20 ng/mL)and AFP-group(AFP<20 ng/mL),comparing the levels of NLR/PLR/MLR/SII/SIRI/PNI and prognosis.In AFP+group,the impact of NLR/PLR/MLR/SII/SIRI/PNI and their dynamic changes on prognosis were further explored.RESULTS Compared with AFP-patients,AFP+patients had higher NLR/PLR/MLR/SII/-SIRI and lower PNI levels and poorer overall survival(OS).In the AFP+group,mortality was significantly lower in the lower NLR/PLR/MLR/SII/SIRI group and higher PNI group.Moreover,the dynamic increase(NLR/PLR/MLR/SII/-SIRI)or decrease(PNI)was associated with the rise of mortality within 1 year of follow-up.CONCLUSION Compared with AFP-patients,the level of inflammation-related peripheral blood markers significantly increased in AFP+patients,which was correlated with OS of AFP+patients.Also,the gradual increase of SII and SIRI was associated with the risk of death within one year in AFP+patients.AFPGC should be considered as a separate type and distinguished from AFP-GC because of the difference in tumor immune microenvironment.It requires basic experiments and large clinical samples in the future.
基金supported by Foundation of Shanghai Science and Technology Committee(No.2041195020002)Shanghai Key Laboratory of Traditional Chinese Clinical Medicine(No.14DZ2273200)Shanghai Key Clinical Specialty(No.shslczdzk05101)。
文摘Objective:To observe the early interventions of traditional Chinese Medicine(TCM)on the conversion time of nucleic acid in patients with coronavirus disease 2019(COVID-19),and find possible underlying mechanisms of action.Methods:A retrospective cohort study was conducted on 300 confirmed COVID-19 patients who were treated with TCM,at a designated hospital in China.The patients were categorized into three groups:TCM1,TCM2 and TCM3,who respectively received TCM interventions within 7,8–14,and greater than15 days of hospitalization.Different indicators such as the conversion time of pharyngeal swab nucleic acid,the conversion time of fecal nucleic acid,length of hospital stay,and inflammatory markers(leukocyte count,and lymphocyte count and percentage)were analyzed to observe the impact of early TCM interventions on these groups.Results:The median conversion times of pharyngeal swab nucleic acid in the three groups were 5.5,7 and16 d(P<0.001),with TCM1 and TCM2 being statistically different from TCM3(P<0.01).TCM1(P<0.05)and TCM3(P<0.01)were statistically different from TCM2.The median conversion times of fecal nucleic acid in the three groups were 7,9 and 17 d(P<0.001).Conversion times of fecal nucleic acid in TCM1 were statistically different from TCM3 and TCM2(P<0.01).The median lengths of hospital stay in the three groups were 13,16 and 21 d(P<0.001).TCM1 and TCM2 were statistically different from TCM3(P<0.01);TCM1 and TCM3 were statistically different from TCM2(P<0.01).Both leucocyte and lymphocyte counts increased gradually with an increase in the length of hospital stay in TCM1 group patients,with a statistically significant difference observed at each time point in the group(P<0.001).Statistically significant differences in lymphocyte count and percentage in TCM2(P<0.001),and in leucocyte count(P=0.043)and lymphocyte count(P=0.038)in TCM3 were observed.The comparison among the three groups showed a statistically significant difference in lymphocyte percentage on the third day of admission(P=0.044).Conclusion:In this study,it was observed that in COVID-19 patients treated with a combination of Chinese and Western medicines,TCM intervention earlier in the hospital stay correlated with faster conversion time of pharyngeal swab and fecal nucleic acid,as well as shorter length of hospital stay,thus helping promote faster recovery of the patient.The underlying mechanism of action may be related to improving inflammation in patients with COVID-19.
文摘Objective To analyze the occurrence of metabolic dysfunction-associated fatty liver disease(MAFLD)and related inflammatory indicators in obstructive sleep apnea hypopnea syndrome(OSAHS)and explore the risk factors of MAFLD.Methods This is a cross-sectional study.From January 2022 to October 2024,172 patients with sleep disorders were enrolled in the First Affiliated Hospital of Soochow University,including 38 patients with non-OSAHS,53 patients with mild OSAHS,37 patients with moderate OSAHS,and 44 patients with severe OSAHS.The occurrence of MAFLD was comprehensively judged from three aspects:metabolic dysfunction-associated fatty liver(MAFL),elevated liver enzymes,and liver fibrosis.The situation of MAFLD and the level of related inflammatory markers were compared among the four groups.Binary logistic regression was used to analyze the risk factors for MAFLD in OSAHS.Results There were significant differences in the prevalence of MAFL,the percentage of elevated liver enzymes,and interleukin-6 and tumor necrosis factoralpha levels among the four groups(P<0.05).The differences of fibrosis-4 index and C-reactive protein among the four groups were not statistically significant(P>0.05).Binary logistic regression showed that BMI,triglycerides,longest time of sleep apnea and tumor necrosis factor-alpha were the risk factors for MAFL(P<0.05).BMI,glucose,and apnea-hypopnea index were the risk factors for elevated liver enzymes(P<0.05).Conclusion OSAHS is strongly associated with MAFLD,and the involvement of OSAHS in the occurrence and development of MAFLD may be related to obesity,lipid metabolism disorders,insulin resistance,inflammatory responses,and intermittent hypoxia.
基金the Agencia Española de Medicamentos y Productos Sanitarios(ref:PLJ-BEV-2016-01)the Hospital Universitario de Gran Canaria Doctor Negrin committee(ref:170007).
文摘Aim:It remains unclear what the best therapeutic option for recurrent glioma patients after Stupp treatment is.Bevacizumab(BVZ)is commonly administered in progression,but it appears that only some patients benefit.It would be useful to find biomarkers that determine beforehand who these patients are.Methods:The protocol included 31 high-risk progressing glioma patients after Stupp treatment who received BVZ 5-10 mg/kg every 14 days and temozolomide(3-19 cycles,150-200 mg five days each 28-day cycle)during a mean of eight cycles of BVZ or until tumor progression or unacceptable toxicity.We analyzed the clinical outcome values of inflammatory indices measured before BVZ administration.Results:Lymphocyte level before BVZ administration was the best independent predictor of overall survival(HR=0.34;95%CI:0.145-0.81;P=0.015).The area under the receiver operating characteristic(ROC)curve was 0.823,with 1.645 being the optimal cut-off value,and 0.80 and 0.85 the sensitivity and specificity values,respectively.Responder and non-responder survival curves were also significantly different,considering the first and second tertiles as cut-off points.The number of BVZ cycles was not related to lymphopenia.Pretreatment neutrophil platelet levels,platelet-to-lymphocyte ratio(PLR),and neutrophil-to-lymphocyte ratio(NLR)did not have independent predictive value.Inflammatory variables were not correlated with each other.However,patients with high NLR and PLR simultaneously(double positive PLR-NLR)showed a worse clinical outcome than the rest(P=0.043).Conclusion:Pretreatment lymphocyte levels and double positive PLR-NLR could be used as non-invasive hematological prognostic markers for recurrent gliomas treated with bevacizumab.A close relationship emerged between inflammation and angiogenesis.
