The development of deep reservoirs is an emerging topic in the energy industry.This paper analyzes the challenges in simulations of flow and transport in deep reservoirs and introduces models and algorithms aimed at r...The development of deep reservoirs is an emerging topic in the energy industry.This paper analyzes the challenges in simulations of flow and transport in deep reservoirs and introduces models and algorithms aimed at resolving these challenges.A fast,accurate,and robust phase equilibrium model is developed with the aid of deep learning algorithms to accelerate the thermodynamic analysis of deep reservoir fluids.A pixel-free search algorithm is developed to generate a pore-network model that describes pore connectivity and porous media fluidity.A fully conservative Implicit Pressure Explicit Saturation algorithm is developed to simulate the Darcy-scale two-phase flow while achieving a reliable result for production evaluation.Numerical examples are presented to validate the performance of the developed models and algorithms.This paper also presents suggestions for future studies on deep reservoirs to achieve both scientific and engineering progress.展开更多
BACKGROUND Differential diagnosis among atypical hyperplasia(AH)(including reparative hyperplasia and intestinal metaplasia),low-grade dysplasia(LGD),high-grade dysplasia(HGD),and adenocarcinoma(AC)in gastric mucosal ...BACKGROUND Differential diagnosis among atypical hyperplasia(AH)(including reparative hyperplasia and intestinal metaplasia),low-grade dysplasia(LGD),high-grade dysplasia(HGD),and adenocarcinoma(AC)in gastric mucosal biopsies is challenging due to histomorphological overlaps,variability in pathological diagnosis consistency,and limited reproducibility.AIM To evaluate the diagnostic utility of P53,Ki67,P504S,and IMP3 in gastric cancer and its precancerous lesions,focusing on their effectiveness in distinguishing AH,LGD,HGD,and AC.METHODS From January 2018 to September 2020,a total of 185 gastric mucosal biopsy specimens were analyzed according to the pathological diagnostic criteria outlined in the World Health Organization Classification of Digestive System Tumors(2019).The specimens were categorized into four groups:AH,LGD,HGD,and AC.Immunohistochemistry was employed to assess the expression status of P53,Ki67,P504S,and IMP3.Intergroup comparisons were performed using theχ^(2)test or Fisher's exact probability test to compare the differences in immunohistochemical markers across the distinct lesion groups.RESULTS The expression rate of P504S was highest in the LGD group(53.3%,16/30),while IMP3 expression was highest in the AC group(41.9%,26/62),followed by the HGD group(33.3%).Significant differences in P504S and IMP3 expression levels were observed among the four lesion groups(P<0.001).Pairwise comparisons revealed statistically significant differences in P504S expression between the AH group and the LGD,HGD,and AC groups(P<0.001),as well as significant variations in IMP3 expression between the AH group and the HGD and AC groups,and between the LGD group and the HGD and AC groups(P<0.001).Additionally,significant correlations were found between P504S and the polarity expression pattern of Ki67,and between IMP3 and the mutation expression pattern of P53(P<0.001).The combined detection of P504S with Ki67 and IMP3 with P53 increased the diagnostic sensitivity for LGD and HGD/AC,respectively.CONCLUSION P504S is highly expressed in LGD and is associated with the Ki67“polarity”expression pattern.IMP3 is highly expressed in HGD/AC and is correlated with the P53 mutation expression pattern.The combined detection of P504S with Ki67 and IMP3 with P53 increased the diagnostic sensitivity for LGD and HGD/AC,respectively.The rational use of P504S,Ki67,IMP3,and p53 can help distinguish gastric cancer and precancerous lesions,improving the early cancer diagnosis rate.展开更多
RNA contains diverse post-transcriptional modifications,and its catabolic breakdown yields numerous modified nucleosides requiring correct processing,but the mechanisms remain unknown.Here,we demonstrate that three RN...RNA contains diverse post-transcriptional modifications,and its catabolic breakdown yields numerous modified nucleosides requiring correct processing,but the mechanisms remain unknown.Here,we demonstrate that three RNA-derived modified adenosines,N6-methyladenosine(m6A),N6,N6-dimethyladenosine(m6,6A),and N6-isopentenyladenosine(i6A),are sequentially metabolized into inosine monophosphate(IMP)to mitigate their intrinsic cytotoxicity.展开更多
The characteristics of deposition of chicken IMP and IMF were investigated in this experiment. Arbor Acres (AA) and Beijing Fatty Chickens (n=200, respectively) were raised under the same condition and were slaughtere...The characteristics of deposition of chicken IMP and IMF were investigated in this experiment. Arbor Acres (AA) and Beijing Fatty Chickens (n=200, respectively) were raised under the same condition and were slaughtered at 28 d, 56 d and 90 d to assay the content of IMP and IMF in breast and leg muscle. The results showed that the IMP content of Beijing Fatty Chicken was significantly higher than that of AA (P<0.01), and also significant difference existed between them in IMF (P<0.01). The IMP content in breast muscle was higher than that in leg muscle (P<0.01), while the IMF was inverse (P<0.01). IMP content of female was a little bit higher than that of male (P>0.05). IMF content varied with growth rate, and showed no association with sex. IMF content increased with age, while IMP did not.展开更多
基金King Abdullah University of Science and Technology,Grant/Award Numbers:BAS/1/1351-01,URF/1/5028-01National Natural Science Foundation of China,Grant/Award Number:51936001。
文摘The development of deep reservoirs is an emerging topic in the energy industry.This paper analyzes the challenges in simulations of flow and transport in deep reservoirs and introduces models and algorithms aimed at resolving these challenges.A fast,accurate,and robust phase equilibrium model is developed with the aid of deep learning algorithms to accelerate the thermodynamic analysis of deep reservoir fluids.A pixel-free search algorithm is developed to generate a pore-network model that describes pore connectivity and porous media fluidity.A fully conservative Implicit Pressure Explicit Saturation algorithm is developed to simulate the Darcy-scale two-phase flow while achieving a reliable result for production evaluation.Numerical examples are presented to validate the performance of the developed models and algorithms.This paper also presents suggestions for future studies on deep reservoirs to achieve both scientific and engineering progress.
基金Supported by The Science and Technology Research Project of Anyang,No.2022C01SF074。
文摘BACKGROUND Differential diagnosis among atypical hyperplasia(AH)(including reparative hyperplasia and intestinal metaplasia),low-grade dysplasia(LGD),high-grade dysplasia(HGD),and adenocarcinoma(AC)in gastric mucosal biopsies is challenging due to histomorphological overlaps,variability in pathological diagnosis consistency,and limited reproducibility.AIM To evaluate the diagnostic utility of P53,Ki67,P504S,and IMP3 in gastric cancer and its precancerous lesions,focusing on their effectiveness in distinguishing AH,LGD,HGD,and AC.METHODS From January 2018 to September 2020,a total of 185 gastric mucosal biopsy specimens were analyzed according to the pathological diagnostic criteria outlined in the World Health Organization Classification of Digestive System Tumors(2019).The specimens were categorized into four groups:AH,LGD,HGD,and AC.Immunohistochemistry was employed to assess the expression status of P53,Ki67,P504S,and IMP3.Intergroup comparisons were performed using theχ^(2)test or Fisher's exact probability test to compare the differences in immunohistochemical markers across the distinct lesion groups.RESULTS The expression rate of P504S was highest in the LGD group(53.3%,16/30),while IMP3 expression was highest in the AC group(41.9%,26/62),followed by the HGD group(33.3%).Significant differences in P504S and IMP3 expression levels were observed among the four lesion groups(P<0.001).Pairwise comparisons revealed statistically significant differences in P504S expression between the AH group and the LGD,HGD,and AC groups(P<0.001),as well as significant variations in IMP3 expression between the AH group and the HGD and AC groups,and between the LGD group and the HGD and AC groups(P<0.001).Additionally,significant correlations were found between P504S and the polarity expression pattern of Ki67,and between IMP3 and the mutation expression pattern of P53(P<0.001).The combined detection of P504S with Ki67 and IMP3 with P53 increased the diagnostic sensitivity for LGD and HGD/AC,respectively.CONCLUSION P504S is highly expressed in LGD and is associated with the Ki67“polarity”expression pattern.IMP3 is highly expressed in HGD/AC and is correlated with the P53 mutation expression pattern.The combined detection of P504S with Ki67 and IMP3 with P53 increased the diagnostic sensitivity for LGD and HGD/AC,respectively.The rational use of P504S,Ki67,IMP3,and p53 can help distinguish gastric cancer and precancerous lesions,improving the early cancer diagnosis rate.
文摘RNA contains diverse post-transcriptional modifications,and its catabolic breakdown yields numerous modified nucleosides requiring correct processing,but the mechanisms remain unknown.Here,we demonstrate that three RNA-derived modified adenosines,N6-methyladenosine(m6A),N6,N6-dimethyladenosine(m6,6A),and N6-isopentenyladenosine(i6A),are sequentially metabolized into inosine monophosphate(IMP)to mitigate their intrinsic cytotoxicity.
文摘The characteristics of deposition of chicken IMP and IMF were investigated in this experiment. Arbor Acres (AA) and Beijing Fatty Chickens (n=200, respectively) were raised under the same condition and were slaughtered at 28 d, 56 d and 90 d to assay the content of IMP and IMF in breast and leg muscle. The results showed that the IMP content of Beijing Fatty Chicken was significantly higher than that of AA (P<0.01), and also significant difference existed between them in IMF (P<0.01). The IMP content in breast muscle was higher than that in leg muscle (P<0.01), while the IMF was inverse (P<0.01). IMP content of female was a little bit higher than that of male (P>0.05). IMF content varied with growth rate, and showed no association with sex. IMF content increased with age, while IMP did not.
