Objective To evaluate the long-term therapeutic effect and histologic result of ADM combined with autologous thin split-thickness skin graft.Methods 23 patients were treated with acellalar dermal matrix(ADM) combined ...Objective To evaluate the long-term therapeutic effect and histologic result of ADM combined with autologous thin split-thickness skin graft.Methods 23 patients were treated with acellalar dermal matrix(ADM) combined with autoiogous展开更多
Demyelination and remyelination play key roles in spinal cord injury(SCI),affecting the recovery of motor and sensory functions.Research in rodent models is extensive,but the study of these processes in non-human prim...Demyelination and remyelination play key roles in spinal cord injury(SCI),affecting the recovery of motor and sensory functions.Research in rodent models is extensive,but the study of these processes in non-human primates is limited.Therefore,our goal was to thoroughly study the histological features of demyelination and remyelination after contusion injury of the cervical spinal cord in Macaca fascicularis.In a previous study,we created an SCI model in M.fascicularis by controlling the contusion displacement.We used Eriochrome Cyanine staining,immunohistochemical analysis,and toluidine blue staining to evaluate demyelination and remyelination.The results showed demyelination ipsilateral to the injury epicenter both rostrally and caudally,the former mainly impacting sensory pathways,while the latter primarily affected motor pathways.Toluidine blue staining showed myelin loss and axonal distension at the injury site.Schwann cell-derived myelin sheaths were only found at the center,while thinner myelin sheaths from oligodendrocytes were seen at the center and surrounding areas.Our study showed that long-lasting demyelination occurs in the spinal cord of M.fascicularis after SCI,with oligodendrocytes and Schwann cells playing a significant role in myelin sheath formation at the injury site.展开更多
Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting ...Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting or mini-cutting. We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA （indole-3-butyric acid） concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings. For the first experiment, the mini-cuttings were stored at 4℃ for 0 （immediate planting）, 24, 48, 72, 96 and 120 h. The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse. The basal region of the mini-cutting was treated with various IBA solutions： 0 （free of IBA）, 1,000, 2,000, 3,000 and 4,000 mg.L^-1. Every seven days （0 （immediate planting）, 7, 14, 21 and 28 days）, destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting. Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots. The 2,000 mg.L^-1 IBA concentration induced a greater speed and percentage of adventitious rooting, and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse. Expo- sure to low temperature induced adventitious root formation with diffuse vascular connections.展开更多
Sulfate chitosan derivatives have good solubility and therapeutic effect on the cell model of NAFLD. The aim of this study was to examine the therapeutic effect of sulfate chitosan derivatives on NAFLD. The male Wista...Sulfate chitosan derivatives have good solubility and therapeutic effect on the cell model of NAFLD. The aim of this study was to examine the therapeutic effect of sulfate chitosan derivatives on NAFLD. The male Wistar rats were orally fed high fat emulsion and received sulfate chitosan derivatives for 5 weeks to determine the pre-treatment effect of sulfate chitosan derivatives on NAFLD. To evaluate the therapeutic effect of sulfate chitosan derivatives on NAFLD, the rats were orally fed with high concentration emulsion for 5 weeks, followed by sulfate chitosan derivatives for 3 weeks. Histological analysis and biomedical assays showed that sulfate chitosan derivatives can dramatically prevent the development of hepatic steatosis in hepatocyte cells. In animal studies, pre-treatment and treatment with sulfate chitosan derivatives significantly protected against hepatic steatohepatitis induced by high fat diet according to histological analysis. Furthermore, increased TC, ALT, MDA, and LEP in NAFLD were significantly ameliorated by pre-treatment and treatment with sulfate chitosan derivatives. Furthermore, increased TG, AST, and TNF-α in NAFLD were significantly ameliorated by treatment with sulfate chitosan derivatives. Sulfate chitosan derivatives have good pre-treatment and therapeutic effect on NAFLD.展开更多
This randomized clinical trial aimed to compare volumetric changes and histological characteristics of new bone in extraction sockets grafted with Bio-Oss^(R)or THE Graft^(R).Furthermore,it sought to further assess th...This randomized clinical trial aimed to compare volumetric changes and histological characteristics of new bone in extraction sockets grafted with Bio-Oss^(R)or THE Graft^(R).Furthermore,it sought to further assess the quality and quantity of bone regeneration at 3-and 6-month intervals.Thirty-two patients requiring single-tooth extraction(excluding third molars)and ridge preservation in preparation for subsequent implant placement were randomized to receive either graft material(Bio-Oss^(R)or THE Graft^(R))covered by a collagen membrane.At 3 months,BV/TV was higher in the DBBM groups than in the DPBM groups,while mineralized bone proportions were similar between groups.By 6 months,BV/TV remained significantly higher in DPBM groups than in the DBBM groups,and a higher proportion of mineralized bone was observed in the DPBM groups.Both groups showed increasing BV/TV and mineralized bone over time.In conclusion,both Bio-Oss^(R)and THE Graft^(R)effectively promoted socket bone regeneration,with healing time influencing bone formation.Long-term results suggest THE Graft®may enhance mineralized bone percentage more than Bio-Oss^(R).展开更多
Many egg-laying reptiles possess temperature-dependent sex determination(TSD)in which outcome of gonadogenesis is determined by incubation temperature during a temperature-sensitive period of development.Prior studies...Many egg-laying reptiles possess temperature-dependent sex determination(TSD)in which outcome of gonadogenesis is determined by incubation temperature during a temperature-sensitive period of development.Prior studies on Malayemys macrocephala showed that incubation temperatures influence gonadal development and suggested that M.macrocephala exhibits TSD.However,information on the temperature-sensitivity period in this species was unknown until the current study.Turtle eggs were collected from rice fields in central Thailand from December 2016 to February 2017.In the laboratory,eggs were incubated at male-biased temperature(26°C)and shifted to female-biased temperature(32°C),or vice versa.Single shift experiments were performed systematically during embryonic stages 13–21.After hatching,sex of individual turtles was determined by histological analysis.We found that the sex determination of M.macrocephala is affected by temperature up to stage 16 of embryonic development.展开更多
The present study was conducted to evaluate the impact of calcium oxide-nanoparticles(CaO-NPs)administration at different concentrations on the epithelial thickness,papilla length and number of blood vessels of male W...The present study was conducted to evaluate the impact of calcium oxide-nanoparticles(CaO-NPs)administration at different concentrations on the epithelial thickness,papilla length and number of blood vessels of male Wister rats’buccal mucosa,and the epithelial thickness,filiform length and number of blood vessels of the rats’tongue.Twelve rats were used and randomly allocated into four groups:control group(untreated)and the three experimental groups were orally treated with CaO-NPs at different doses 25,50 and 100 mg/kg of the body weight over two months.On the 61st day,all the animals were sacrificed,and 1 cm of the buccal mucosa and the body of the tongue were carefully removed for histological analysis.Histological tissues were studied under a light microscope to investigate the impact of CaO-NPs administration on the oral tissues.An image processing software(Image J)was used to measure the epithelial thickness,papilla length and number of blood vessels of the rats’buccal mucosa,and the epithelial thickness,filiform length and number of blood vessels of the rats’tongue.The results showed that the CaO nanoparticles administration caused epithelial atrophy and decreased vascularization of buccal and tongue mucosa with all tested doses of CaO-NPs and the 100 mg/kg concentration showed the most significant effect.展开更多
文摘Objective To evaluate the long-term therapeutic effect and histologic result of ADM combined with autologous thin split-thickness skin graft.Methods 23 patients were treated with acellalar dermal matrix(ADM) combined with autoiogous
基金supported by the National Natural Science Foundation of China(81972064)the Guangdong Basic and Applied Basic Research Foundation(2021A1515111117,2020A1515011415).
文摘Demyelination and remyelination play key roles in spinal cord injury(SCI),affecting the recovery of motor and sensory functions.Research in rodent models is extensive,but the study of these processes in non-human primates is limited.Therefore,our goal was to thoroughly study the histological features of demyelination and remyelination after contusion injury of the cervical spinal cord in Macaca fascicularis.In a previous study,we created an SCI model in M.fascicularis by controlling the contusion displacement.We used Eriochrome Cyanine staining,immunohistochemical analysis,and toluidine blue staining to evaluate demyelination and remyelination.The results showed demyelination ipsilateral to the injury epicenter both rostrally and caudally,the former mainly impacting sensory pathways,while the latter primarily affected motor pathways.Toluidine blue staining showed myelin loss and axonal distension at the injury site.Schwann cell-derived myelin sheaths were only found at the center,while thinner myelin sheaths from oligodendrocytes were seen at the center and surrounding areas.Our study showed that long-lasting demyelination occurs in the spinal cord of M.fascicularis after SCI,with oligodendrocytes and Schwann cells playing a significant role in myelin sheath formation at the injury site.
基金financially by FAPESP (So Paulo Research Foundation,Brazil)CAPES (Coordination for the Improvement of Higher Level Personnel,Brazil)
文摘Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting or mini-cutting. We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA （indole-3-butyric acid） concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings. For the first experiment, the mini-cuttings were stored at 4℃ for 0 （immediate planting）, 24, 48, 72, 96 and 120 h. The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse. The basal region of the mini-cutting was treated with various IBA solutions： 0 （free of IBA）, 1,000, 2,000, 3,000 and 4,000 mg.L^-1. Every seven days （0 （immediate planting）, 7, 14, 21 and 28 days）, destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting. Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots. The 2,000 mg.L^-1 IBA concentration induced a greater speed and percentage of adventitious rooting, and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse. Expo- sure to low temperature induced adventitious root formation with diffuse vascular connections.
基金supported by the National High Technology Research and Development Program of China (863 Program 2006AA090401)
文摘Sulfate chitosan derivatives have good solubility and therapeutic effect on the cell model of NAFLD. The aim of this study was to examine the therapeutic effect of sulfate chitosan derivatives on NAFLD. The male Wistar rats were orally fed high fat emulsion and received sulfate chitosan derivatives for 5 weeks to determine the pre-treatment effect of sulfate chitosan derivatives on NAFLD. To evaluate the therapeutic effect of sulfate chitosan derivatives on NAFLD, the rats were orally fed with high concentration emulsion for 5 weeks, followed by sulfate chitosan derivatives for 3 weeks. Histological analysis and biomedical assays showed that sulfate chitosan derivatives can dramatically prevent the development of hepatic steatosis in hepatocyte cells. In animal studies, pre-treatment and treatment with sulfate chitosan derivatives significantly protected against hepatic steatohepatitis induced by high fat diet according to histological analysis. Furthermore, increased TC, ALT, MDA, and LEP in NAFLD were significantly ameliorated by pre-treatment and treatment with sulfate chitosan derivatives. Furthermore, increased TG, AST, and TNF-α in NAFLD were significantly ameliorated by treatment with sulfate chitosan derivatives. Sulfate chitosan derivatives have good pre-treatment and therapeutic effect on NAFLD.
基金supported by Oral Fusion major projects of Binzhou Medical University(KQRH2024MS005)Dentsply Sirona,Mölndal,Sweden(IIS No.I-AS-21-030),the National Natural Science Foundation of China(Grant No.12472301)the Fundamental Research Funds for the Central Universities.
文摘This randomized clinical trial aimed to compare volumetric changes and histological characteristics of new bone in extraction sockets grafted with Bio-Oss^(R)or THE Graft^(R).Furthermore,it sought to further assess the quality and quantity of bone regeneration at 3-and 6-month intervals.Thirty-two patients requiring single-tooth extraction(excluding third molars)and ridge preservation in preparation for subsequent implant placement were randomized to receive either graft material(Bio-Oss^(R)or THE Graft^(R))covered by a collagen membrane.At 3 months,BV/TV was higher in the DBBM groups than in the DPBM groups,while mineralized bone proportions were similar between groups.By 6 months,BV/TV remained significantly higher in DPBM groups than in the DBBM groups,and a higher proportion of mineralized bone was observed in the DPBM groups.Both groups showed increasing BV/TV and mineralized bone over time.In conclusion,both Bio-Oss^(R)and THE Graft^(R)effectively promoted socket bone regeneration,with healing time influencing bone formation.Long-term results suggest THE Graft®may enhance mineralized bone percentage more than Bio-Oss^(R).
基金This research was supported by the 90th Anniversary of Chulalongkorn University Fund(Ratchadaphiseksomphot Endowment Fund)An educa-tional grant from the Human Resource Development in Science Project(Science Achievement Scholarship of Thailand)the Research Assistantship Fund from Fac-ulty of Science,Chulalongkorn University,to R.P.are fully acknowledged.
文摘Many egg-laying reptiles possess temperature-dependent sex determination(TSD)in which outcome of gonadogenesis is determined by incubation temperature during a temperature-sensitive period of development.Prior studies on Malayemys macrocephala showed that incubation temperatures influence gonadal development and suggested that M.macrocephala exhibits TSD.However,information on the temperature-sensitivity period in this species was unknown until the current study.Turtle eggs were collected from rice fields in central Thailand from December 2016 to February 2017.In the laboratory,eggs were incubated at male-biased temperature(26°C)and shifted to female-biased temperature(32°C),or vice versa.Single shift experiments were performed systematically during embryonic stages 13–21.After hatching,sex of individual turtles was determined by histological analysis.We found that the sex determination of M.macrocephala is affected by temperature up to stage 16 of embryonic development.
基金the support from University of Kufa to provide the experimental facility.
文摘The present study was conducted to evaluate the impact of calcium oxide-nanoparticles(CaO-NPs)administration at different concentrations on the epithelial thickness,papilla length and number of blood vessels of male Wister rats’buccal mucosa,and the epithelial thickness,filiform length and number of blood vessels of the rats’tongue.Twelve rats were used and randomly allocated into four groups:control group(untreated)and the three experimental groups were orally treated with CaO-NPs at different doses 25,50 and 100 mg/kg of the body weight over two months.On the 61st day,all the animals were sacrificed,and 1 cm of the buccal mucosa and the body of the tongue were carefully removed for histological analysis.Histological tissues were studied under a light microscope to investigate the impact of CaO-NPs administration on the oral tissues.An image processing software(Image J)was used to measure the epithelial thickness,papilla length and number of blood vessels of the rats’buccal mucosa,and the epithelial thickness,filiform length and number of blood vessels of the rats’tongue.The results showed that the CaO nanoparticles administration caused epithelial atrophy and decreased vascularization of buccal and tongue mucosa with all tested doses of CaO-NPs and the 100 mg/kg concentration showed the most significant effect.
