目的：通过高效液相色谱-飞行时间质谱（HPLC-TOF/MS）联用技术定性分析莱菔子中的主要化学成分。方法：色谱分离采用Diamonsil-C18柱（2.1 mm×150 mm,3μm）;流动相组成为乙腈-水（含0.1%醋酸）,梯度洗脱,流速为0.3 m L/min,质谱...目的：通过高效液相色谱-飞行时间质谱（HPLC-TOF/MS）联用技术定性分析莱菔子中的主要化学成分。方法：色谱分离采用Diamonsil-C18柱（2.1 mm×150 mm,3μm）;流动相组成为乙腈-水（含0.1%醋酸）,梯度洗脱,流速为0.3 m L/min,质谱定性采用飞行时间质谱,正负离子模式扫描。结果：快速鉴别出莱菔子中13种化学成分。结论：建立了一种简单可靠的HPLC-TOF/MS方法对莱菔子中主要化学成分进行了鉴定。展开更多
According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac effica...According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac efficacy of alkaloids in Aconitum tubers. First, the chemical ingredients in unprocessed and processed Aconitum tubers were identified and compared by using high performance liquid chromatography time-of-flight mass spectrometry(HPLC-TOF/MS) and multivariate pattern recognition methods. Then the representative alkaloids in Aconitum tubers, aconitine, benzoylaconine, and aconine, which belong to diester-diterpenoid alkaloids,monoester-diterpenoid alkaloids, and amine-diterpenoid alkaloids, respectively, were selected for further validation of attenuated mechanism. Subsequent pharmacological experiments with aconitine, benzoylaconine,and aconine in SD rats were used to validate the effect of processing on cardiac functions. After processing the Aconitum tubers, it was found that the contents of diester-diterpenoid alkaloids were reduced, and those of monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids were increased, suggesting that diesterditerpenoid alkaloids were transformed into monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids.Through further decocting the aconitine in boiling water, it was confirmed that the three alkaloids could be progressively transformed. Pharmacological experiments with aconitine, benzoylaconine, and aconine in SD rats showed that aconitine at a dose of 0.01 mg/kg and aconine at a dose of 10 mg/kg enhanced the cardiac function, while benzoylaconine at a dose of 2 mg/kg weakened the cardiac function. The effect of processing is attributed to the transformation of the most toxic diester-diterpenoid alkaloids into less toxic monoesterditerpenoid alkaloids and amine-diterpenoid alkaloids.展开更多
文摘目的：通过高效液相色谱-飞行时间质谱（HPLC-TOF/MS）联用技术定性分析莱菔子中的主要化学成分。方法：色谱分离采用Diamonsil-C18柱（2.1 mm×150 mm,3μm）;流动相组成为乙腈-水（含0.1%醋酸）,梯度洗脱,流速为0.3 m L/min,质谱定性采用飞行时间质谱,正负离子模式扫描。结果：快速鉴别出莱菔子中13种化学成分。结论：建立了一种简单可靠的HPLC-TOF/MS方法对莱菔子中主要化学成分进行了鉴定。
基金financially supported by the National Natural Science Foundation of China (81573396)Military Innovation Funding (16CXZ012)
文摘According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac efficacy of alkaloids in Aconitum tubers. First, the chemical ingredients in unprocessed and processed Aconitum tubers were identified and compared by using high performance liquid chromatography time-of-flight mass spectrometry(HPLC-TOF/MS) and multivariate pattern recognition methods. Then the representative alkaloids in Aconitum tubers, aconitine, benzoylaconine, and aconine, which belong to diester-diterpenoid alkaloids,monoester-diterpenoid alkaloids, and amine-diterpenoid alkaloids, respectively, were selected for further validation of attenuated mechanism. Subsequent pharmacological experiments with aconitine, benzoylaconine,and aconine in SD rats were used to validate the effect of processing on cardiac functions. After processing the Aconitum tubers, it was found that the contents of diester-diterpenoid alkaloids were reduced, and those of monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids were increased, suggesting that diesterditerpenoid alkaloids were transformed into monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids.Through further decocting the aconitine in boiling water, it was confirmed that the three alkaloids could be progressively transformed. Pharmacological experiments with aconitine, benzoylaconine, and aconine in SD rats showed that aconitine at a dose of 0.01 mg/kg and aconine at a dose of 10 mg/kg enhanced the cardiac function, while benzoylaconine at a dose of 2 mg/kg weakened the cardiac function. The effect of processing is attributed to the transformation of the most toxic diester-diterpenoid alkaloids into less toxic monoesterditerpenoid alkaloids and amine-diterpenoid alkaloids.
