The present study was designed to investigate the influence of the pretreatment of piperazine ferulate on pharmacokinetic parameters of methotrexate in methotrexate-induced renal injury rats.A simple and efficient hig...The present study was designed to investigate the influence of the pretreatment of piperazine ferulate on pharmacokinetic parameters of methotrexate in methotrexate-induced renal injury rats.A simple and efficient high performance liquid chromatography coupled with mass spectrometry(HPLC-MS)method was developed to determine methotrexate in rat plasma.Methotrexate and syringic acid(internal standard)were extracted from rat plasma samples by protein precipitation with acetonitrile.The analysis was performed on a CAPCELL PAK C18column(150 mm×4.6 mm,5μm)with acetonitrile and 5 mmol/l ammonium acetate aqueous(10:90,v/v).The linear range was 5.0×10-2to 100.0μg/ml for methotrexate.Other parameters were all within the acceptance criteria.The validated method was successfully applied the pharmacokinetic study of methotrexate between two methotrexate treated groups(with and without the pretreatment of piperazine ferulate).Compared with the methotrexate treated alone group,the pharmacokinetic parameters in the methotrexate with the pretreatment of piperazine ferulate group showed significantly lower MRT(0-t),MRT(0-∞) and T1/2.Results suggested that methotrexate can be rapidly eliminated,cleared or metabolized in rat blood,which might be related to the pretreatment of piperazine ferulate.The method provided deeper insights into rational clinical use of methotrexate with the pretreatment of piperazine ferulate on cancer patients with renal dysfunction.展开更多
目的探讨不同梯度洗脱条件下HPLC图谱特征峰位位移变化的规律,快速建立优化的图谱条件。方法采用HPLC法,以大黄为模型药材,比较两个不同梯度洗脱条件下特征峰的总洗脱液溶度参数。色谱柱:Alltech Apollo C18柱(250 mm×4.6 mm,5μm)...目的探讨不同梯度洗脱条件下HPLC图谱特征峰位位移变化的规律,快速建立优化的图谱条件。方法采用HPLC法,以大黄为模型药材,比较两个不同梯度洗脱条件下特征峰的总洗脱液溶度参数。色谱柱:Alltech Apollo C18柱(250 mm×4.6 mm,5μm);流动相:乙腈-水-1%冰醋酸,梯度洗脱;检测波长:254 nm;柱温:40℃;进样体积10μL。结果大黄13个相匹配的特征峰,在初始程序和优化程序下,各自的总洗脱液溶度参数基本相等,其中1号匹配峰的偏差最大,为-12.05%,4号匹配峰的偏差最小,为-0.388%。结论HPLC图谱在不同梯度洗脱条件下特征峰位位移遵循总洗脱液溶度参数相等的规律。展开更多
文摘The present study was designed to investigate the influence of the pretreatment of piperazine ferulate on pharmacokinetic parameters of methotrexate in methotrexate-induced renal injury rats.A simple and efficient high performance liquid chromatography coupled with mass spectrometry(HPLC-MS)method was developed to determine methotrexate in rat plasma.Methotrexate and syringic acid(internal standard)were extracted from rat plasma samples by protein precipitation with acetonitrile.The analysis was performed on a CAPCELL PAK C18column(150 mm×4.6 mm,5μm)with acetonitrile and 5 mmol/l ammonium acetate aqueous(10:90,v/v).The linear range was 5.0×10-2to 100.0μg/ml for methotrexate.Other parameters were all within the acceptance criteria.The validated method was successfully applied the pharmacokinetic study of methotrexate between two methotrexate treated groups(with and without the pretreatment of piperazine ferulate).Compared with the methotrexate treated alone group,the pharmacokinetic parameters in the methotrexate with the pretreatment of piperazine ferulate group showed significantly lower MRT(0-t),MRT(0-∞) and T1/2.Results suggested that methotrexate can be rapidly eliminated,cleared or metabolized in rat blood,which might be related to the pretreatment of piperazine ferulate.The method provided deeper insights into rational clinical use of methotrexate with the pretreatment of piperazine ferulate on cancer patients with renal dysfunction.
