This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms.Cell viability was meas...This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms.Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.The induction of apoptosis was detected by light and transmission electron microscopy and flow cytometry.Western blot analysis was used to investigate the related mechanisms.Nude mice were further employed to investigate the antitumour activity of ART in vivo.MTT assay results demonstrated that ART selectively inhibits the growth of HOS cells in a dose-and time-dependent manner.Based on the findings of light and transmission electron microscopy,Hoechst 33258 staining,and fluorescein isothiocyanate (FITC)-annexin V staining,the cytotoxicity of ART in HOS cells occurs through apoptosis.With ART treatment,cytosolic cytochrome c was increased,Bax expression was gradually upregulated,Bcl-2 expression was downregulated,and caspase-9 and caspase-3 were activated.Thus,the intrinsic apoptotic pathway may be involved in ART-induced apoptosis.Cell cycle analysis by flow cytometry indicated that ART may induce cell cycle arrest at G2 /M phase.In nude mice bearing HOS xenograft tumours,ART inhibited tumour growth and regulated the expressions of cleaved caspase-3 and survivin,in agreement with in vitro observations.ART has a selective antitumour activity against human osteosarcoma HOS cells,which may be related to its effects on induction of apoptosis via the intrinsic pathway.The results suggest that ART is a promising candidate for the treatment of osteosarcoma.展开更多
The goal of this work was aimed to improve the power conversion efficiency of single crystalline silicon-based photovoltaic cells by using the solar spectral conversion principle, which employs an up-conversion phosph...The goal of this work was aimed to improve the power conversion efficiency of single crystalline silicon-based photovoltaic cells by using the solar spectral conversion principle, which employs an up-conversion phosphor to convert a low energy infrared photon to the more energetic visible photons to improve the spectral response. In this study, the surface of multicrystalline silicon solar cells was coated with an up-conversion molybdate phosphor to improve the spectral response of the solar cell in the near-infrared spectral range. The short circuit current （Isc）, open circuit voltage （Voc）, and conversion efficiency （η） of spectral conversion cells were measured. Preliminary experimental results revealed that the light conversion efficiency of a 1.5%–2.7% increase in Si-based cell was achieved.展开更多
This paper presents a joint high order statistics （HOS） and signal-to-noise ratio （SNR） algorithm for the recognition of multiple-input multiple-output （MIMO） radar signal without a priori knowledge of the signa...This paper presents a joint high order statistics （HOS） and signal-to-noise ratio （SNR） algorithm for the recognition of multiple-input multiple-output （MIMO） radar signal without a priori knowledge of the signal parameters. This method is capable of recognizing the MIMO radar signal as well as discriminating it from single-carrier signal adopted by conventional radar. Meanwhile, the sub-carrier number of the none-coding MIMO radar signal is estimated. Extensive simulations are carried out in different operating conditions. Simulation results prove the feasibility and indicate that the recognition probability could reach over 90% when the value of SNR is above 0 dB.展开更多
文摘This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms.Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.The induction of apoptosis was detected by light and transmission electron microscopy and flow cytometry.Western blot analysis was used to investigate the related mechanisms.Nude mice were further employed to investigate the antitumour activity of ART in vivo.MTT assay results demonstrated that ART selectively inhibits the growth of HOS cells in a dose-and time-dependent manner.Based on the findings of light and transmission electron microscopy,Hoechst 33258 staining,and fluorescein isothiocyanate (FITC)-annexin V staining,the cytotoxicity of ART in HOS cells occurs through apoptosis.With ART treatment,cytosolic cytochrome c was increased,Bax expression was gradually upregulated,Bcl-2 expression was downregulated,and caspase-9 and caspase-3 were activated.Thus,the intrinsic apoptotic pathway may be involved in ART-induced apoptosis.Cell cycle analysis by flow cytometry indicated that ART may induce cell cycle arrest at G2 /M phase.In nude mice bearing HOS xenograft tumours,ART inhibited tumour growth and regulated the expressions of cleaved caspase-3 and survivin,in agreement with in vitro observations.ART has a selective antitumour activity against human osteosarcoma HOS cells,which may be related to its effects on induction of apoptosis via the intrinsic pathway.The results suggest that ART is a promising candidate for the treatment of osteosarcoma.
基金Project supported by National Science Council of Taiwan (NSC98-2113-M-009-005-MY3)
文摘The goal of this work was aimed to improve the power conversion efficiency of single crystalline silicon-based photovoltaic cells by using the solar spectral conversion principle, which employs an up-conversion phosphor to convert a low energy infrared photon to the more energetic visible photons to improve the spectral response. In this study, the surface of multicrystalline silicon solar cells was coated with an up-conversion molybdate phosphor to improve the spectral response of the solar cell in the near-infrared spectral range. The short circuit current （Isc）, open circuit voltage （Voc）, and conversion efficiency （η） of spectral conversion cells were measured. Preliminary experimental results revealed that the light conversion efficiency of a 1.5%–2.7% increase in Si-based cell was achieved.
基金supported by the Foundation of Chinese People’s Liberation Army General Equipment Department(41101020303)
文摘This paper presents a joint high order statistics （HOS） and signal-to-noise ratio （SNR） algorithm for the recognition of multiple-input multiple-output （MIMO） radar signal without a priori knowledge of the signal parameters. This method is capable of recognizing the MIMO radar signal as well as discriminating it from single-carrier signal adopted by conventional radar. Meanwhile, the sub-carrier number of the none-coding MIMO radar signal is estimated. Extensive simulations are carried out in different operating conditions. Simulation results prove the feasibility and indicate that the recognition probability could reach over 90% when the value of SNR is above 0 dB.
