Monitoring changes in Annual Net Primary Productivity(ANPP)is required for reporting on UN Sustainable Development Goal(SDG)Indicator 15.3.1:the proportion of land that is degraded over the total land area.Calibrating...Monitoring changes in Annual Net Primary Productivity(ANPP)is required for reporting on UN Sustainable Development Goal(SDG)Indicator 15.3.1:the proportion of land that is degraded over the total land area.Calibrating time-series observations of ANPP to derive Water Use Efficiency(WUE;a measure of ANPP per unit of evapotranspiration)can minimize the influence of climate factors on ANPP observations and highlight the influence of non-climatic drivers of degradation such as land use changes.Comparing the ANPP and WUE time series may be useful for identifying the primary drivers of land degradation,which could be used to support the Land Degradation Neutrality objectives of the UN Convention to Combat Desertification(UNCCD).This paper presents an algorithm for the Google Earth Engine(freely and openly available upon request-http://doi.org/10.5281/zenodo.4429773)to calculate and compare ANPP and WUE time series for Santa Cruz,Bolivia,which has recently experienced an intensification in its land use.This code builds on the Good Practice Guidance document(ver-sion 1)for monitoring SDG Indicator 15.3.1.We use the MODIS 16-day average,250 m resolution to demonstrate that the Enhanced Vegetation Index(EVI)responds faster to changes in water avail-ability than the Normalized Difference Vegetation Index(NDVI).We also consider the relationships between ANPP and WUE.Significant and concordant trends may highlight good agricultural practices or increased resilience in ecosystem structure and productivity when they are positive or reducing resilience and functional integrity if negative.The sign and significance of the correlation between ANPP and WUE may also diverge over time.With further analysis,it may be possible to interpret this relationship in terms of the drivers of change in plant productivity and ecosystem resilience.展开更多
At present time when climate change has negative effect on soil moisture and can decrease significantly the productivity, good agricultural practises have a high importance via their direct influence on soil propertie...At present time when climate change has negative effect on soil moisture and can decrease significantly the productivity, good agricultural practises have a high importance via their direct influence on soil properties, regimes and biodiversity. Objectives of this study have been focused on the assessment of good agricultural practises in different soil cultivation types: conventional, minimum till, mulch, no-till and organic farming. Method used was based on two case study areas where organic and/or minimal farming systems have been applied. As a control, we chose soil with traditional cultivation. In organic farm, we evaluated earthworms;their amount and status and in farm with different types of cultivation we evaluated the microbial activity to assess the biodiversity conditions. Basic soil properties and soil structure have been set to be able to assess the influence of good agricultural practises on soil environment. Our study shows positive effect of these practises on soil moisture content, biodiversity and soil structure stability. These findings can be used for further studies determining the ways of soil cultivation in harmony with nature—in sustainable way.展开更多
Haiti is the poorest country in the Western Hemisphere and presents a unique scenario for the food and agriculture industry, because there is no food safety legislation. The application of Good Agricultural Practices ...Haiti is the poorest country in the Western Hemisphere and presents a unique scenario for the food and agriculture industry, because there is no food safety legislation. The application of Good Agricultural Practices (GAPs) leads to improvements of quality, safety and sustainability of agricultural products. The purpose of the study was to assess the status of Good Agricultural Practices (GAPs) in cocoa and coffee farms in Northern Haiti. A general survey captured information about the farmer and the farm, and an audit checklist was used to assess compliance to GAPs. A total of 11 farms (n = 11) were audited, of which 7 were cocoa farms (64%) and 4 were coffee farms (34%) in the regions of Dondon, Limonade and Milot. Average overall audit scores for coffee farms (73%) were higher than for cocoa farms (55%). Farms affiliated with a cooperative scored higher (78%) than those that were not part of a cooperative (55%). The sections of the survey on “Practices related to premises and production site”, and the “use of agricultural inputs and chemicals” received the lowest scores but were confined to the cocoa farms. “Record keeping” plus “distribution, transportation, and traceability” were cause for concern with both the cocoa and coffee farms. Critical non-conformances included the access of livestock animals and domestic pets to processing and storage areas, the lack of control in the application of agricultural chemicals, a lack of safeguards on equipment and elevated surfaces, and washing of fresh cocoa beans to remove the mucilage with water that had not been treated or tested for potability. The root cause of the non-conformances, regardless of the commodity, was either related to poor physical and organizational infrastructures, or to a lack of technical training.展开更多
An earlier baseline study （Jellason et al. in preparation） has identified some of the key challenges faced by farmers and from that, appropriate Good Agricultural Practices （GAPs） have been selected for the enviro...An earlier baseline study （Jellason et al. in preparation） has identified some of the key challenges faced by farmers and from that, appropriate Good Agricultural Practices （GAPs） have been selected for the environment the farmers find themselves in and based on a review of available literature. Farmer action plans for enhancing smallholder resilience, GHGs emission mitigation and food security for these dry lands were developed. Expert opinions on the applicability of the selected GAPs to inform farmer co-learning and to validate the suitability of these practices in a dry land context were sought through the Delphi study. Experts were also invited to offer suggestions for improvement of the training action plan. Two rounds of a modified Delphi survey were carried out with experts from diverse backgrounds and locations across the globe to solicit their opinion. Results show that experts＇ agreement was reached on most of the action plan items. The results and opinions obtained from the survey were reviewed and adapted into the action plan leading to the development of GAPs for the co-learning exercise.展开更多
BACKGROUND Good clinical practice(GCP)is put in place to protect human participants in clinical trials as well as to ensure the quality of research.Non-adherence to these guidelines can produce research that may not m...BACKGROUND Good clinical practice(GCP)is put in place to protect human participants in clinical trials as well as to ensure the quality of research.Non-adherence to these guidelines can produce research that may not meet the standards set by the scientific community.Therefore,it must be ensured that researchers are wellversed in the GCP.But not much is known about the knowledge and practices of the GCP in the medical colleges of North India.AIM To assess the knowledge and practices of researchers about GCP and analyze these with respect to the demographics of participants.METHODS This is a cross-sectional study.A self-structured questionnaire about GCP,after expert validations,was circulated among researchers,at a tertiary healthcare institute,All India Institute of Medical Sciences(AIIMS),Rishikesh.A total of 59 individuals,who were selected by universal sampling,participated in the study.All healthcare workers who have been investigators of Institutional Ethics Committee-approved research projects,except residents and faculty,and are still a part of the institute have been included in the study.The study was approved by the Institutional Ethics Committee of AIIMS,Rishikesh.We used descriptive analysis and the Chi-squared test to analyze data.P value<0.05 was considered significant.RESULTS Out of 59 participants,only 11(18.6%)were certified for GCP.Most of the participants(64.4%)had“Average”knowledge,33.9%had“Good”knowledge and 1.7%had“Poor”knowledge.Only 49%of participants had satisfactory practices related to GCP.There was a significant difference in the knowledge based on the current academic position for the items assessing knowledge of institutional review board(P=0.010),confidentiality&privacy(P=0.011),and participant safety&adverse events(P<0.001).There was also a significant difference in knowledge of research misconduct(P=0.024)and participant safety&adverse events(P=0.011)based on certification of GCP.There was a notable difference in the practices related to recruitment&retention on the basis of current academic position(P<0.001)and certification of GCP(P=0.023).We also observed a considerable difference between the knowledge and practices of GCP among the participants(P=0.013).CONCLUSION Participants have basic knowledge of GCP but show a lack thereof in certain domains of GCP.This can be addressed by holding training sessions focusing on these particular domains.展开更多
In today’s world,higher education,especially in technical sciences,is crucial when speaking about a change in values and attitudes towards sustainability.Engaging students in learning and training process as well as ...In today’s world,higher education,especially in technical sciences,is crucial when speaking about a change in values and attitudes towards sustainability.Engaging students in learning and training process as well as developing their research skills and creative capacity is challenging.This study inquiries into the current academic activities and role of used educational technologies and methodologies for encouraging active learning in both undergraduate and postgraduate programs when teaching any engineering course in general,and geospatial sciences in particular.The result of the study suggests the importance of interdisciplinary project and problem-based learning,building partnership with universities,students and industrial companies,monitoring,developing,and implementation of new educational technologies.展开更多
Corporate social responsibility （CSR） is becoming more and more frequently emphasized part of management in the structure of Polish enterprises. These results from the fact that growing awareness of consumers, destr...Corporate social responsibility （CSR） is becoming more and more frequently emphasized part of management in the structure of Polish enterprises. These results from the fact that growing awareness of consumers, destructive impact of industry on the natural environment, economic scandals, unethical behavior among entrepreneurs, bring about redefining the principles of a business activity on the market by entrepreneurs. As a result of the above, such values as honesty, trust, or responsibility take on greater importance. Additionally, more and more often, Polish entrepreneurs pay attention to the influence of the company on the environment, the way the stakeholders are treated, while striving to maximize profit. The present paper looks into the subject of social responsibility from the point of view of Polish enterprises. To verify this objective, there was the analysis of the due literature performed, and also carried out the survey among 174 entrepreneurs running their business activity in the area of Southern Poland. The research tool used in the research was the questionnaire （PAPI and CAWI）. In the conducted research, there were factors analyzed referring to knowledge of social responsibility among the people holding both managerial and lower-level positions, in particular opinion, is it important for enterprises to be driven by ethical standards？ How to implement CSR in their structures？ How to understood concept of CSR？ What are the reasons for the implementation of CSR in the strategy of the company？ The considerations （both the theoretical and practical ones） confirmed that the Polish enterprises feel the increased need to implement the activities in the field of social responsibility, which, in the present conditions, constitutes an important part of running a business activity.展开更多
The aim of the study was to examine microorganisms in samples of fer<span style="font-family:;" "="">mented milk beverage and water from dairy under state inspection. In a dairy located ...The aim of the study was to examine microorganisms in samples of fer<span style="font-family:;" "="">mented milk beverage and water from dairy under state inspection. In a dairy located in the Rec<span style="color:#4F4F4F;font-family:-apple-system, "font-size:16px;white-space:normal;background-color:#FFFFFF;">ô</span>ncavo of Bahia, fifteen samples of fermented milk beverage were <span>collected, corresponding to three different batches, and six water samples</span>, two from each point of use in the industry: Point 1</span><span style="font-family:;" "="">—<span>Sanitary barrier;Point <span>2</span></span><span>—<span>Internal faucet for cleaning surfaces and utensils;Point 3</span>—<span>Drinking</span></span><span> fountain for employees. All samples were submitted to microbiological research of total coliforms, <i>Escherichia coli</i> and <i>Aeromonas</i> spp. In the water samples, there was also quantification of heterotrophic bacteria and analysis of free residual chlorine. The fermented milk beverage samples did not show growth of total coliforms, <i>E. coli</i> and <i>Aeromonas</i> spp. With regard to water, no sample showed measurable levels of free residual chlorine, while in the microbiolog<span>ical analysis the count of heterotrophic bacteria in the drinking water exceeded the limit established by legislation (5.6 </span></span><span>×<span> 10<sup>2</sup> CFU<span style="color:#4F4F4F;font-family:-apple-system, "font-size:16px;white-space:normal;background-color:#F7F7F7;">.</span>mL<sup>-1</sup>). In addition, the water from the sanitary barrier revealed total coliforms above the allowed</span></span><span> <span>value (1.3 </span></span><span>× <span>10<sup>1</sup> CFU<span style="color:#4F4F4F;font-family:-apple-system, "font-size:16px;white-space:normal;background-color:#F7F7F7;">.</span>100 mL<sup>-1</sup>) and showed contamination by <i>Aeromonas</i></span></span><span> spp. (7.0 </span>×<span> 10<sup>1</sup> CFU<span style="color:#4F4F4F;font-family:-apple-system, "font-size:16px;white-space:normal;background-color:#F7F7F7;">.</span>mL<sup>-1</sup>). Thus, it can be inferred that, although t<span>he fermented milk beverage did not present the investigated microorganisms, the </span><span>water used by the industry showed microbial contamination, and </span><span>due to its participation in the entire production process, water quality control is extremely important to prevent contamination of the food </span><span>handler and the</span><span> final product, preventing the spread of diseases and harm to public health.</span></span></span>展开更多
A road safety management (RSM) system can be defined as “a complex institutional structure that involves cooperating and interacting bodies which support the tasks and processes necessary for the prevention and reduc...A road safety management (RSM) system can be defined as “a complex institutional structure that involves cooperating and interacting bodies which support the tasks and processes necessary for the prevention and reduction of road traffic injuries”. RSM should promote the road safety progress of the country. However, the details of this relationship are generally lacking. This study explored the RSM systems in European countries based on the information collected through interviews with experts and officials, in each country, and using a “good practice” criteria questionnaire. The dataset included 14 countries with fifty items related to five RSM areas: institutional organization;policy formulation and adoption;policy implementation and funding;monitoring and evaluation;scientific support, information and capacity building. Cluster analyses and correlations were used to identify country groups with similar RSM components, to recognize typical RSM structures if available and to examine the relationship between RSM and road safety performance of the countries. The findings showed that all the countries are different when RSM systems are considered as a whole, making it impossible to identify typical RSM structures or a single best working model at a national level. However, it is possible to compare countries when the RSM areas are considered separately, where the clusters of countries recognized by the study present the patterns common for those European countries. Across the analyses, a number of countries with a consistently higher and lower availability of the RSM components were identified, enabling a final countries’ ranking into a number of groups. The latter actually reflects the level of RSM in the country, in terms of its correspondence to the “good practice” criteria. A further analysis indicated a positive correlation between the higher level of the RSM system and better safety performance of the countries.展开更多
The progress of tissue-engineering technology has realized development of new therapies to treat various disorders by using cultured cells. Cell-and tissue-based therapies have been successfully applied to human patie...The progress of tissue-engineering technology has realized development of new therapies to treat various disorders by using cultured cells. Cell-and tissue-based therapies have been successfully applied to human patients, and several tissue-engineered products have been approved by the regulatory agencies and are commercially available. In the review article, we describe our experience of development and clinical application of cell sheet-based regenerative medicine.Endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) have been shown to be useful for removal of gastrointestinal neoplasms with less invasiveness compared with open surgery, especially in esophageal surgery. However, postoperative inflammation and stenosis are major complications observed after intensive mucosal resection. Therefore, we have developed novel regenerative medicine to prevent such complications and promote wound healing of esophageal mucosa after EMR or ESD. Transplantable oral mucosal epithelial cell sheets were fabricated from patients' own oral mucosa. Immediately after EMR or ESD, fabricated autologous cell sheets were endoscopically transplanted to the ulcer sites. We performed a preclinical study with a canine model. In human clinical settings, cell culture and cell sheet fabrication were performed in clean rooms according to good manufacturing practice guidelines, and pharmaceutical drugs were used as supplements to culture medium in place of research regents used in animal study. We believe that cell-based regenerative medicine would be useful to improve quality of life of patients after EMR or ESD.展开更多
Claudin18.2(CLDN18.2)is a tight junction protein that is overexpressed in a variety of solid tumors such as gastrointestinal cancer and oesophageal cancer.It has been identified as a promising target and a potential b...Claudin18.2(CLDN18.2)is a tight junction protein that is overexpressed in a variety of solid tumors such as gastrointestinal cancer and oesophageal cancer.It has been identified as a promising target and a potential biomarker to diagnose tumor,evaluate efficacy,and determine patient prognosis.TST001 is a recombinant humanized CLDN18.2 antibody that selectively binds to the extracellular loop of human Claudin18.2.In this study,we constructed a solid target radionuclide zirconium-^(89)(^(89)Zr)labled-TST001 to detect the expression of in the human stomach cancer BGC823CLDN18.2 cell lines.The[^(89)Zr]Zr-desferrioxamine(DFO)-TST001 showed high radiochemical purity(RCP,>99%)and specific activity(24.15±1.34 GBq/mmol),and was stable in 5%human serum albumin,and phosphate buffer saline(>85%RCP at 96 h).The EC_(50) values of TST001 and DFO-TST001 were as high as 0.413±0.055 and 0.361±0.058 nM(P>0.05),respectively.The radiotracer had a significantly higher average standard uptake values in CLDN18.2-positive tumors than in CLDN18.2-negative tumors(1.11±0.02 vs.0.49±0.03,P=0.0016)2 days post injection(p.i.).BGC823CLDN18.2 mice models showed high tumor/muscle ratios 96 h p.i.with[^(89)Zr]Zr-DFO-TST001 was much higher than those of the other imaging groups.Immunohistochemistry results showed that BGC823CLDN18.2 tumors were highly positive(+++)for CLDN18.2,while those in the BGC823 group did not express CLDN18.2().The results of ex vivo biodistribution studies showed that there was a higher distribution in the BGC823CLDN18.2 tumor bearing mice(2.05±0.16%ID/g)than BGC823 mice(0.69±0.02%ID/g)and blocking group(0.72±0.02%ID/g).A dosimetry estimation study showed that the effective dose of[^(89)Zr]Zr-DFO-TST001 was 0.0705 mSv/MBq,which is within the range of acceptable doses for nuclear medicine research.Taken together,these results suggest that Good Manufacturing Practices produced by this immuno-positron emission tomography probe can detect CLDN18.2-overexpressing tumors.展开更多
Traditional herbal medicine(THM)is an important part of the traditional Chinese medicine culture.Due to its high medicinal potential,it should not only serve for the Chinese people’s medical use,but also contribute t...Traditional herbal medicine(THM)is an important part of the traditional Chinese medicine culture.Due to its high medicinal potential,it should not only serve for the Chinese people’s medical use,but also contribute to the world medicine,THM for the international market must be standardized and large-scale,and produced according to the"Good Agriculture Practice"(GAP).The quality of THM directly affects the patient’s treatment status and safety of use.Therefore,the quality assurance of THM runs through the entire process of research and development,production and clinical practice.The standardized production and cultivation of THM is the starting point of the THM industry chain and plays a decisive role in the economic development of the THM industry.This article summarizes the development history,limitations and future development of GAP,and clarifies the opportunities for THM in the rapid development of the international and domestic Chinese medicine industry.In addition,analyzing the deficiencies that were existing in the former GAP implementation process and by suggesting science-based quality measures,it is hoped to stipulate improved GAP guidelines in the future and to lay the foundation for a modern THM international trade.展开更多
Mesenchymal stem stromal cells(MSC)are characterized by the intriguing capacity to home toward cancer cells after systemic administration.Thus,MSC can be harnessed as targeted delivery vehicles of cytotoxic agents aga...Mesenchymal stem stromal cells(MSC)are characterized by the intriguing capacity to home toward cancer cells after systemic administration.Thus,MSC can be harnessed as targeted delivery vehicles of cytotoxic agents against tumors.In cancer patients,MSC based advanced cellular therapies were shown to be safe but their clinical efficacy was limited.Indeed,the amount of systemically infused MSC actually homing to human cancer masses is insufficient to reduce tumor growth.Moreover,induction of an unequivocal anticancer cytotoxic phenotype in expanded MSC is necessary to achieve significant therapeutic efficacy.Ex vivo cell modifications are,thus,required to improve anti-cancer properties of MSC.MSC based cellular therapy products must be handled in compliance with good manufacturing practice(GMP)guidelines.In the present review we include MSCimproving manipulation approaches that,even though actually tested at preclinical level,could be compatible with GMP guidelines.In particular,we describe possible approaches to improve MSC homing on cancer,including genetic engineering,membrane modification and cytokine priming.Similarly,we discuss appropriate modalities aimed at inducing a marked cytotoxic phenotype in expanded MSC by direct chemotherapeutic drug loading or by genetic methods.In conclusion,we suggest that,to configure MSC as a powerful weapon against cancer,combinations of clinical grade compatible modification protocols that are currently selected,should be introduced in the final product.Highly standardized cancer clinical trials are required to test the efficacy of ameliorated MSC based cell therapies.展开更多
To evaluate and improve the milk quality in dual purpose cattle with hand or machine milking, a good management and sanitary program was implemented （GMSP） in 40 farms. Was obtained a diagnostic by an interview and ...To evaluate and improve the milk quality in dual purpose cattle with hand or machine milking, a good management and sanitary program was implemented （GMSP） in 40 farms. Was obtained a diagnostic by an interview and in situ evaluation of management conditions and milk samples for laboratory analysis integrating the platform and microbiological counts, before and after of GMSP. The changes achieved were no significant （P 〉 0.05） for pH and density, but alcohol and foreign material were positive modified （P 〈 0.05） by GMSP in both hand and machine milking. The quantities of sediments were decreased （P 〈 0.05） in more than 50.0%. Decrease （P 〈 0.05） was observed in the counts of CFU mL1 （colony forming unitsl） in aerobic mesophilic, coliforms, Salmonella ssp. and Staphylococcus aureus with the GMSP. After GMSP Salmonella ssp. incidence was affected （P 〈 0.05） by hand （55.5%） vs. machine （61.5%） milking type; coliforms count and positive incidence in milk were affected （P 〈 0.05） in hand （81.5%） vs. machine （53.9%）. Therefore it was confirmed that the implementation of adequate sanitary and management practices, in both hand and machine milking, decreased the presence and incidence of microorganisms in milk with potential to produce disease in animals and humans.展开更多
A'simplified'European procedure now allows the registration of traditional herbal medicines as medicinal products even without the support of clinical data.This procedure entails the requirement that those pro...A'simplified'European procedure now allows the registration of traditional herbal medicines as medicinal products even without the support of clinical data.This procedure entails the requirement that those products comply with European Good Manufacturing Practice for medicinal products,which in turn implies that the raw herbal materials comply with the European Guidelines for Good Agricultural and Collection Practice.On the basis of a comparison between European Good Agricultural and Collection Practice and China Good Agricultural Practice,as well as direct observation made at sites in China,we issue some recommendations to facilitate good communication between the Chinese producer and European pharmaceutical customer,with a view to ensure full compliance with European expectations.展开更多
Training and dissemination of good handling practices are key points in reducing animal abuse. Three aspects are considered in the development of education in animal welfare （AW）： （1） introduction of AW in curric...Training and dissemination of good handling practices are key points in reducing animal abuse. Three aspects are considered in the development of education in animal welfare （AW）： （1） introduction of AW in curricular education, nevertheless it has not been applied in the Facultad de Veterinaria, Universidad de la Repfiblica de Uruguay, up to now; （2） promotion of permanent education for graduates; （3） development of plans for dissemination, follow up, assessment and continuous improvement on AW affairs. Aiming to obtain information about the knowledge of veterinarians on AW during the practice of their professional duties, in September 2016, an on-line questionnaire was sent to the veterinarians who were in the Educaci6n Continua database. The questionnaire had four sections： a general gathering information and three additional sections, one for each veterinarian professional field （large animal＇s health and production, small animal＇s health and food industry）. From 1,804 questionnaires sent, 388 answers were obtained. There were 69% attended at least one AW course, and 89.9% were willing to attend another AW course. And 63.5% considered the information received to be very useful for their daily activities, 95% considered that AW should be included in the veterinary studies curriculum, while 53.3% declared not having knowledge about the regional animal welfare strategy （RAWS）. The majority of the veterinarians considered the acquired knowledge very useful and are eager to obtain more information. It is necessary to keep working to include AW in the veterinary studies curriculum as well as continuing with the diffusion of good practices and AW principles, based on the national and international norms.展开更多
The area of interest is located in the South Moravia Region at the confluence of the Jihlava, Oslava and Rokytn^i rivers. Ivan^ice spring area comprises a series of hydrologic boreholes, it is the main water source fo...The area of interest is located in the South Moravia Region at the confluence of the Jihlava, Oslava and Rokytn^i rivers. Ivan^ice spring area comprises a series of hydrologic boreholes, it is the main water source for water supply of Ivan^ice and Rosice towns and provides water for 30,000 inhabitants. The risk analysis was created on the basis of water quality monitoring, hydrogeological assessment and terrain exploration and a revision of protection zones was proposed. The spring area is situated in nitrate vulnerable zones and recently nitrate concentrations have been decreasing. Water quality evaluation results： high concentration of manganese and iron, sometimes higher concentration of ammonium and COD. This area is intensively used for agriculture and it is necessary to make a compromise solution during protection zones proposal, The regime in protection zones can not affect manganese and iron concentration （their origin is in the natural geological environment）. Therefore, water treatment plant is in operation and its modernization is proposed. Furthermore, the paper deals with spring area intensification construction of a new hydrologic borehole, and managed and unmanaged infiltration of surface water. The proposal of protection zones revision consists of reduction to a 2nd level protection zone.展开更多
The aim of the research was to evaluate overall quality of introduction of hazard analysis and critical control points （HACCP） prerequisites and HACCP procedures in catering sector in Latvia to reveal common problem...The aim of the research was to evaluate overall quality of introduction of hazard analysis and critical control points （HACCP） prerequisites and HACCP procedures in catering sector in Latvia to reveal common problems that can provoke food-borne infection outbreaks. Certain quantitative indicators were defined and analyzed to conclude about characteristic trends related to implementation of food safety legislation. The state＇s monitoring data on surveillance of catering establishments were used. Fulfillment of legislative requirements in about 6,000 catering establishments was evaluated during time period of 2004-2009. The results of investigation were obtained with help of statistical analyses using MS Excel computer program. The results of the research suggest that most of catering establishments （e.g., 83.6% during 2009） meet the hygiene requirements only partly. Non-conformities regarding HACCP-based self-control procedures were fixed during 49.2% of inspections. The research reveals characteristic shortcomings that are established from year to year, e.g., improper hygiene of premises （established during 30%-40% of inspections, depending on the year）, poor hygiene of technological equipment （established during 27%-37% of inspections）, poor personnel hygiene （established during 11%-22% of inspections）, inadequate competence of food service personnel （established during 10%-35% of inspections）, as well as non-observance of cleaning-disinfection procedures and technological parameters for food processing （established during 11%-25% of inspections, depending on the year）. Actual shortcomings can lead to both the direct and the indirect cross-contamination of food. On the base of the research, it was concluded that further detailed assessment of potential food safety risks is relevant to establish risk-based monitoring activities in catering sector.展开更多
This research developed the technology which united ISO-GUM （International Organization of Standard-Guide to Express of Uncertainty in Measurement） and QE （Quality Engineer） strategically and employed both feature...This research developed the technology which united ISO-GUM （International Organization of Standard-Guide to Express of Uncertainty in Measurement） and QE （Quality Engineer） strategically and employed both feature efficiently. Although to tow technology of purpose use is same, each has future. The purpose is scheduled to improve the accuracy as a result of the clinical examinations. The result of research was used for diagnosis by equivalent Quality Control （eqQC） of the same level when and where, that are needed for broader-based medical system of the time of IT-era. Since National Institute of Standard and Technology （NIST） is recommend use of complicated operation by ISO-GUM for uniting, realization required the newest high quality computer technology. The algorithm of the complexity system of operation is that Fast Fourier Transform （FFT）, Inverse Fast Fourier Transform （IFFT） and Multi-variance Analysis （MA） are needed in Fig. 4. The main new improving points are changed of ambiguous expression method of data, and the pursuit which the cause of worsens accuracy put into data. All data with an assurance reported the final result for patient or donor. Medical laboratory quality system service is offered essential in the laboratory to be correct result for health care by Good Laboratory Practices （GLP） useful ISO 15189. Quality Assurance （QA） is achieved though system reliability.展开更多
基金This study was partially funded by UNDP Grant:BOL/118208(“Laboratorios de Recuperación Temprana”),a study led by Fundación para Conservación del Bosque Chiquitano(www.fcbc.org.bo)to determine forest patches requiring post-fire assisted recovery in the aftermath of 2019 wildfires in Santa Cruz,Bolivia:“Plan Estratégico para la Restauración de lasÁreas Afectadas por los Incendios en el 2019”.
文摘Monitoring changes in Annual Net Primary Productivity(ANPP)is required for reporting on UN Sustainable Development Goal(SDG)Indicator 15.3.1:the proportion of land that is degraded over the total land area.Calibrating time-series observations of ANPP to derive Water Use Efficiency(WUE;a measure of ANPP per unit of evapotranspiration)can minimize the influence of climate factors on ANPP observations and highlight the influence of non-climatic drivers of degradation such as land use changes.Comparing the ANPP and WUE time series may be useful for identifying the primary drivers of land degradation,which could be used to support the Land Degradation Neutrality objectives of the UN Convention to Combat Desertification(UNCCD).This paper presents an algorithm for the Google Earth Engine(freely and openly available upon request-http://doi.org/10.5281/zenodo.4429773)to calculate and compare ANPP and WUE time series for Santa Cruz,Bolivia,which has recently experienced an intensification in its land use.This code builds on the Good Practice Guidance document(ver-sion 1)for monitoring SDG Indicator 15.3.1.We use the MODIS 16-day average,250 m resolution to demonstrate that the Enhanced Vegetation Index(EVI)responds faster to changes in water avail-ability than the Normalized Difference Vegetation Index(NDVI).We also consider the relationships between ANPP and WUE.Significant and concordant trends may highlight good agricultural practices or increased resilience in ecosystem structure and productivity when they are positive or reducing resilience and functional integrity if negative.The sign and significance of the correlation between ANPP and WUE may also diverge over time.With further analysis,it may be possible to interpret this relationship in terms of the drivers of change in plant productivity and ecosystem resilience.
文摘At present time when climate change has negative effect on soil moisture and can decrease significantly the productivity, good agricultural practises have a high importance via their direct influence on soil properties, regimes and biodiversity. Objectives of this study have been focused on the assessment of good agricultural practises in different soil cultivation types: conventional, minimum till, mulch, no-till and organic farming. Method used was based on two case study areas where organic and/or minimal farming systems have been applied. As a control, we chose soil with traditional cultivation. In organic farm, we evaluated earthworms;their amount and status and in farm with different types of cultivation we evaluated the microbial activity to assess the biodiversity conditions. Basic soil properties and soil structure have been set to be able to assess the influence of good agricultural practises on soil environment. Our study shows positive effect of these practises on soil moisture content, biodiversity and soil structure stability. These findings can be used for further studies determining the ways of soil cultivation in harmony with nature—in sustainable way.
文摘Haiti is the poorest country in the Western Hemisphere and presents a unique scenario for the food and agriculture industry, because there is no food safety legislation. The application of Good Agricultural Practices (GAPs) leads to improvements of quality, safety and sustainability of agricultural products. The purpose of the study was to assess the status of Good Agricultural Practices (GAPs) in cocoa and coffee farms in Northern Haiti. A general survey captured information about the farmer and the farm, and an audit checklist was used to assess compliance to GAPs. A total of 11 farms (n = 11) were audited, of which 7 were cocoa farms (64%) and 4 were coffee farms (34%) in the regions of Dondon, Limonade and Milot. Average overall audit scores for coffee farms (73%) were higher than for cocoa farms (55%). Farms affiliated with a cooperative scored higher (78%) than those that were not part of a cooperative (55%). The sections of the survey on “Practices related to premises and production site”, and the “use of agricultural inputs and chemicals” received the lowest scores but were confined to the cocoa farms. “Record keeping” plus “distribution, transportation, and traceability” were cause for concern with both the cocoa and coffee farms. Critical non-conformances included the access of livestock animals and domestic pets to processing and storage areas, the lack of control in the application of agricultural chemicals, a lack of safeguards on equipment and elevated surfaces, and washing of fresh cocoa beans to remove the mucilage with water that had not been treated or tested for potability. The root cause of the non-conformances, regardless of the commodity, was either related to poor physical and organizational infrastructures, or to a lack of technical training.
文摘An earlier baseline study （Jellason et al. in preparation） has identified some of the key challenges faced by farmers and from that, appropriate Good Agricultural Practices （GAPs） have been selected for the environment the farmers find themselves in and based on a review of available literature. Farmer action plans for enhancing smallholder resilience, GHGs emission mitigation and food security for these dry lands were developed. Expert opinions on the applicability of the selected GAPs to inform farmer co-learning and to validate the suitability of these practices in a dry land context were sought through the Delphi study. Experts were also invited to offer suggestions for improvement of the training action plan. Two rounds of a modified Delphi survey were carried out with experts from diverse backgrounds and locations across the globe to solicit their opinion. Results show that experts＇ agreement was reached on most of the action plan items. The results and opinions obtained from the survey were reviewed and adapted into the action plan leading to the development of GAPs for the co-learning exercise.
文摘BACKGROUND Good clinical practice(GCP)is put in place to protect human participants in clinical trials as well as to ensure the quality of research.Non-adherence to these guidelines can produce research that may not meet the standards set by the scientific community.Therefore,it must be ensured that researchers are wellversed in the GCP.But not much is known about the knowledge and practices of the GCP in the medical colleges of North India.AIM To assess the knowledge and practices of researchers about GCP and analyze these with respect to the demographics of participants.METHODS This is a cross-sectional study.A self-structured questionnaire about GCP,after expert validations,was circulated among researchers,at a tertiary healthcare institute,All India Institute of Medical Sciences(AIIMS),Rishikesh.A total of 59 individuals,who were selected by universal sampling,participated in the study.All healthcare workers who have been investigators of Institutional Ethics Committee-approved research projects,except residents and faculty,and are still a part of the institute have been included in the study.The study was approved by the Institutional Ethics Committee of AIIMS,Rishikesh.We used descriptive analysis and the Chi-squared test to analyze data.P value<0.05 was considered significant.RESULTS Out of 59 participants,only 11(18.6%)were certified for GCP.Most of the participants(64.4%)had“Average”knowledge,33.9%had“Good”knowledge and 1.7%had“Poor”knowledge.Only 49%of participants had satisfactory practices related to GCP.There was a significant difference in the knowledge based on the current academic position for the items assessing knowledge of institutional review board(P=0.010),confidentiality&privacy(P=0.011),and participant safety&adverse events(P<0.001).There was also a significant difference in knowledge of research misconduct(P=0.024)and participant safety&adverse events(P=0.011)based on certification of GCP.There was a notable difference in the practices related to recruitment&retention on the basis of current academic position(P<0.001)and certification of GCP(P=0.023).We also observed a considerable difference between the knowledge and practices of GCP among the participants(P=0.013).CONCLUSION Participants have basic knowledge of GCP but show a lack thereof in certain domains of GCP.This can be addressed by holding training sessions focusing on these particular domains.
文摘In today’s world,higher education,especially in technical sciences,is crucial when speaking about a change in values and attitudes towards sustainability.Engaging students in learning and training process as well as developing their research skills and creative capacity is challenging.This study inquiries into the current academic activities and role of used educational technologies and methodologies for encouraging active learning in both undergraduate and postgraduate programs when teaching any engineering course in general,and geospatial sciences in particular.The result of the study suggests the importance of interdisciplinary project and problem-based learning,building partnership with universities,students and industrial companies,monitoring,developing,and implementation of new educational technologies.
文摘Corporate social responsibility （CSR） is becoming more and more frequently emphasized part of management in the structure of Polish enterprises. These results from the fact that growing awareness of consumers, destructive impact of industry on the natural environment, economic scandals, unethical behavior among entrepreneurs, bring about redefining the principles of a business activity on the market by entrepreneurs. As a result of the above, such values as honesty, trust, or responsibility take on greater importance. Additionally, more and more often, Polish entrepreneurs pay attention to the influence of the company on the environment, the way the stakeholders are treated, while striving to maximize profit. The present paper looks into the subject of social responsibility from the point of view of Polish enterprises. To verify this objective, there was the analysis of the due literature performed, and also carried out the survey among 174 entrepreneurs running their business activity in the area of Southern Poland. The research tool used in the research was the questionnaire （PAPI and CAWI）. In the conducted research, there were factors analyzed referring to knowledge of social responsibility among the people holding both managerial and lower-level positions, in particular opinion, is it important for enterprises to be driven by ethical standards？ How to implement CSR in their structures？ How to understood concept of CSR？ What are the reasons for the implementation of CSR in the strategy of the company？ The considerations （both the theoretical and practical ones） confirmed that the Polish enterprises feel the increased need to implement the activities in the field of social responsibility, which, in the present conditions, constitutes an important part of running a business activity.
文摘The aim of the study was to examine microorganisms in samples of fer<span style="font-family:;" "="">mented milk beverage and water from dairy under state inspection. In a dairy located in the Rec<span style="color:#4F4F4F;font-family:-apple-system, "font-size:16px;white-space:normal;background-color:#FFFFFF;">ô</span>ncavo of Bahia, fifteen samples of fermented milk beverage were <span>collected, corresponding to three different batches, and six water samples</span>, two from each point of use in the industry: Point 1</span><span style="font-family:;" "="">—<span>Sanitary barrier;Point <span>2</span></span><span>—<span>Internal faucet for cleaning surfaces and utensils;Point 3</span>—<span>Drinking</span></span><span> fountain for employees. All samples were submitted to microbiological research of total coliforms, <i>Escherichia coli</i> and <i>Aeromonas</i> spp. In the water samples, there was also quantification of heterotrophic bacteria and analysis of free residual chlorine. The fermented milk beverage samples did not show growth of total coliforms, <i>E. coli</i> and <i>Aeromonas</i> spp. With regard to water, no sample showed measurable levels of free residual chlorine, while in the microbiolog<span>ical analysis the count of heterotrophic bacteria in the drinking water exceeded the limit established by legislation (5.6 </span></span><span>×<span> 10<sup>2</sup> CFU<span style="color:#4F4F4F;font-family:-apple-system, "font-size:16px;white-space:normal;background-color:#F7F7F7;">.</span>mL<sup>-1</sup>). In addition, the water from the sanitary barrier revealed total coliforms above the allowed</span></span><span> <span>value (1.3 </span></span><span>× <span>10<sup>1</sup> CFU<span style="color:#4F4F4F;font-family:-apple-system, "font-size:16px;white-space:normal;background-color:#F7F7F7;">.</span>100 mL<sup>-1</sup>) and showed contamination by <i>Aeromonas</i></span></span><span> spp. (7.0 </span>×<span> 10<sup>1</sup> CFU<span style="color:#4F4F4F;font-family:-apple-system, "font-size:16px;white-space:normal;background-color:#F7F7F7;">.</span>mL<sup>-1</sup>). Thus, it can be inferred that, although t<span>he fermented milk beverage did not present the investigated microorganisms, the </span><span>water used by the industry showed microbial contamination, and </span><span>due to its participation in the entire production process, water quality control is extremely important to prevent contamination of the food </span><span>handler and the</span><span> final product, preventing the spread of diseases and harm to public health.</span></span></span>
文摘A road safety management (RSM) system can be defined as “a complex institutional structure that involves cooperating and interacting bodies which support the tasks and processes necessary for the prevention and reduction of road traffic injuries”. RSM should promote the road safety progress of the country. However, the details of this relationship are generally lacking. This study explored the RSM systems in European countries based on the information collected through interviews with experts and officials, in each country, and using a “good practice” criteria questionnaire. The dataset included 14 countries with fifty items related to five RSM areas: institutional organization;policy formulation and adoption;policy implementation and funding;monitoring and evaluation;scientific support, information and capacity building. Cluster analyses and correlations were used to identify country groups with similar RSM components, to recognize typical RSM structures if available and to examine the relationship between RSM and road safety performance of the countries. The findings showed that all the countries are different when RSM systems are considered as a whole, making it impossible to identify typical RSM structures or a single best working model at a national level. However, it is possible to compare countries when the RSM areas are considered separately, where the clusters of countries recognized by the study present the patterns common for those European countries. Across the analyses, a number of countries with a consistently higher and lower availability of the RSM components were identified, enabling a final countries’ ranking into a number of groups. The latter actually reflects the level of RSM in the country, in terms of its correspondence to the “good practice” criteria. A further analysis indicated a positive correlation between the higher level of the RSM system and better safety performance of the countries.
基金Supported by The Formation of Innovation Center for Fusion of Advanced Technologies in the Special Coordination Funds for Promoting ScienceTechnology from the Ministry of Education, Culture, Sports, Science, and Technology, Japan
文摘The progress of tissue-engineering technology has realized development of new therapies to treat various disorders by using cultured cells. Cell-and tissue-based therapies have been successfully applied to human patients, and several tissue-engineered products have been approved by the regulatory agencies and are commercially available. In the review article, we describe our experience of development and clinical application of cell sheet-based regenerative medicine.Endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) have been shown to be useful for removal of gastrointestinal neoplasms with less invasiveness compared with open surgery, especially in esophageal surgery. However, postoperative inflammation and stenosis are major complications observed after intensive mucosal resection. Therefore, we have developed novel regenerative medicine to prevent such complications and promote wound healing of esophageal mucosa after EMR or ESD. Transplantable oral mucosal epithelial cell sheets were fabricated from patients' own oral mucosa. Immediately after EMR or ESD, fabricated autologous cell sheets were endoscopically transplanted to the ulcer sites. We performed a preclinical study with a canine model. In human clinical settings, cell culture and cell sheet fabrication were performed in clean rooms according to good manufacturing practice guidelines, and pharmaceutical drugs were used as supplements to culture medium in place of research regents used in animal study. We believe that cell-based regenerative medicine would be useful to improve quality of life of patients after EMR or ESD.
基金The research was funded by the National Natural Science Foundation of China(Grant Nos.:82171973,82171980,and 82102092)Beijing Millions of Talent Projects A Level Funding(Grant No.:2019A38)+2 种基金The study was also supported by Beijing Hospitals Authority Dengfeng Project(Grant No.:DFL20191102)the Pilot Project(4th Round)to Reform Public Development of Beijing Municipal Medical Research Institute(20211)the Third Foster Plan in 2019“Molecular Imaging Probe Preparation and Characterization of Key Technologies and Equipment”for the Development of Key Technologies and Equipment in Major Science and Technology Infrastructure in Shenzhen,China.
文摘Claudin18.2(CLDN18.2)is a tight junction protein that is overexpressed in a variety of solid tumors such as gastrointestinal cancer and oesophageal cancer.It has been identified as a promising target and a potential biomarker to diagnose tumor,evaluate efficacy,and determine patient prognosis.TST001 is a recombinant humanized CLDN18.2 antibody that selectively binds to the extracellular loop of human Claudin18.2.In this study,we constructed a solid target radionuclide zirconium-^(89)(^(89)Zr)labled-TST001 to detect the expression of in the human stomach cancer BGC823CLDN18.2 cell lines.The[^(89)Zr]Zr-desferrioxamine(DFO)-TST001 showed high radiochemical purity(RCP,>99%)and specific activity(24.15±1.34 GBq/mmol),and was stable in 5%human serum albumin,and phosphate buffer saline(>85%RCP at 96 h).The EC_(50) values of TST001 and DFO-TST001 were as high as 0.413±0.055 and 0.361±0.058 nM(P>0.05),respectively.The radiotracer had a significantly higher average standard uptake values in CLDN18.2-positive tumors than in CLDN18.2-negative tumors(1.11±0.02 vs.0.49±0.03,P=0.0016)2 days post injection(p.i.).BGC823CLDN18.2 mice models showed high tumor/muscle ratios 96 h p.i.with[^(89)Zr]Zr-DFO-TST001 was much higher than those of the other imaging groups.Immunohistochemistry results showed that BGC823CLDN18.2 tumors were highly positive(+++)for CLDN18.2,while those in the BGC823 group did not express CLDN18.2().The results of ex vivo biodistribution studies showed that there was a higher distribution in the BGC823CLDN18.2 tumor bearing mice(2.05±0.16%ID/g)than BGC823 mice(0.69±0.02%ID/g)and blocking group(0.72±0.02%ID/g).A dosimetry estimation study showed that the effective dose of[^(89)Zr]Zr-DFO-TST001 was 0.0705 mSv/MBq,which is within the range of acceptable doses for nuclear medicine research.Taken together,these results suggest that Good Manufacturing Practices produced by this immuno-positron emission tomography probe can detect CLDN18.2-overexpressing tumors.
基金supported by Ministry of Agriculture and Rural Affairs of the People’s Republic of China(No.CARS-21)National Administration of Traditional Chinese Medicine(No.Finance Society[2019],39)+1 种基金National Natural Science Foundation of China(No.M1942003)National Natural Science Foundation of China(No.M2042003)。
文摘Traditional herbal medicine(THM)is an important part of the traditional Chinese medicine culture.Due to its high medicinal potential,it should not only serve for the Chinese people’s medical use,but also contribute to the world medicine,THM for the international market must be standardized and large-scale,and produced according to the"Good Agriculture Practice"(GAP).The quality of THM directly affects the patient’s treatment status and safety of use.Therefore,the quality assurance of THM runs through the entire process of research and development,production and clinical practice.The standardized production and cultivation of THM is the starting point of the THM industry chain and plays a decisive role in the economic development of the THM industry.This article summarizes the development history,limitations and future development of GAP,and clarifies the opportunities for THM in the rapid development of the international and domestic Chinese medicine industry.In addition,analyzing the deficiencies that were existing in the former GAP implementation process and by suggesting science-based quality measures,it is hoped to stipulate improved GAP guidelines in the future and to lay the foundation for a modern THM international trade.
基金the grant from the Italian Ministry of Health“Ricerca Corrente”funding(J34I19003280007)the organization“Alleanza Contro il Cancro(ACC)(J34I20000600001)the association“Finchèci siete voi ci sono anch'io”(J31I17000440007)”.
文摘Mesenchymal stem stromal cells(MSC)are characterized by the intriguing capacity to home toward cancer cells after systemic administration.Thus,MSC can be harnessed as targeted delivery vehicles of cytotoxic agents against tumors.In cancer patients,MSC based advanced cellular therapies were shown to be safe but their clinical efficacy was limited.Indeed,the amount of systemically infused MSC actually homing to human cancer masses is insufficient to reduce tumor growth.Moreover,induction of an unequivocal anticancer cytotoxic phenotype in expanded MSC is necessary to achieve significant therapeutic efficacy.Ex vivo cell modifications are,thus,required to improve anti-cancer properties of MSC.MSC based cellular therapy products must be handled in compliance with good manufacturing practice(GMP)guidelines.In the present review we include MSCimproving manipulation approaches that,even though actually tested at preclinical level,could be compatible with GMP guidelines.In particular,we describe possible approaches to improve MSC homing on cancer,including genetic engineering,membrane modification and cytokine priming.Similarly,we discuss appropriate modalities aimed at inducing a marked cytotoxic phenotype in expanded MSC by direct chemotherapeutic drug loading or by genetic methods.In conclusion,we suggest that,to configure MSC as a powerful weapon against cancer,combinations of clinical grade compatible modification protocols that are currently selected,should be introduced in the final product.Highly standardized cancer clinical trials are required to test the efficacy of ameliorated MSC based cell therapies.
文摘To evaluate and improve the milk quality in dual purpose cattle with hand or machine milking, a good management and sanitary program was implemented （GMSP） in 40 farms. Was obtained a diagnostic by an interview and in situ evaluation of management conditions and milk samples for laboratory analysis integrating the platform and microbiological counts, before and after of GMSP. The changes achieved were no significant （P 〉 0.05） for pH and density, but alcohol and foreign material were positive modified （P 〈 0.05） by GMSP in both hand and machine milking. The quantities of sediments were decreased （P 〈 0.05） in more than 50.0%. Decrease （P 〈 0.05） was observed in the counts of CFU mL1 （colony forming unitsl） in aerobic mesophilic, coliforms, Salmonella ssp. and Staphylococcus aureus with the GMSP. After GMSP Salmonella ssp. incidence was affected （P 〈 0.05） by hand （55.5%） vs. machine （61.5%） milking type; coliforms count and positive incidence in milk were affected （P 〈 0.05） in hand （81.5%） vs. machine （53.9%）. Therefore it was confirmed that the implementation of adequate sanitary and management practices, in both hand and machine milking, decreased the presence and incidence of microorganisms in milk with potential to produce disease in animals and humans.
文摘A'simplified'European procedure now allows the registration of traditional herbal medicines as medicinal products even without the support of clinical data.This procedure entails the requirement that those products comply with European Good Manufacturing Practice for medicinal products,which in turn implies that the raw herbal materials comply with the European Guidelines for Good Agricultural and Collection Practice.On the basis of a comparison between European Good Agricultural and Collection Practice and China Good Agricultural Practice,as well as direct observation made at sites in China,we issue some recommendations to facilitate good communication between the Chinese producer and European pharmaceutical customer,with a view to ensure full compliance with European expectations.
文摘Training and dissemination of good handling practices are key points in reducing animal abuse. Three aspects are considered in the development of education in animal welfare （AW）： （1） introduction of AW in curricular education, nevertheless it has not been applied in the Facultad de Veterinaria, Universidad de la Repfiblica de Uruguay, up to now; （2） promotion of permanent education for graduates; （3） development of plans for dissemination, follow up, assessment and continuous improvement on AW affairs. Aiming to obtain information about the knowledge of veterinarians on AW during the practice of their professional duties, in September 2016, an on-line questionnaire was sent to the veterinarians who were in the Educaci6n Continua database. The questionnaire had four sections： a general gathering information and three additional sections, one for each veterinarian professional field （large animal＇s health and production, small animal＇s health and food industry）. From 1,804 questionnaires sent, 388 answers were obtained. There were 69% attended at least one AW course, and 89.9% were willing to attend another AW course. And 63.5% considered the information received to be very useful for their daily activities, 95% considered that AW should be included in the veterinary studies curriculum, while 53.3% declared not having knowledge about the regional animal welfare strategy （RAWS）. The majority of the veterinarians considered the acquired knowledge very useful and are eager to obtain more information. It is necessary to keep working to include AW in the veterinary studies curriculum as well as continuing with the diffusion of good practices and AW principles, based on the national and international norms.
文摘The area of interest is located in the South Moravia Region at the confluence of the Jihlava, Oslava and Rokytn^i rivers. Ivan^ice spring area comprises a series of hydrologic boreholes, it is the main water source for water supply of Ivan^ice and Rosice towns and provides water for 30,000 inhabitants. The risk analysis was created on the basis of water quality monitoring, hydrogeological assessment and terrain exploration and a revision of protection zones was proposed. The spring area is situated in nitrate vulnerable zones and recently nitrate concentrations have been decreasing. Water quality evaluation results： high concentration of manganese and iron, sometimes higher concentration of ammonium and COD. This area is intensively used for agriculture and it is necessary to make a compromise solution during protection zones proposal, The regime in protection zones can not affect manganese and iron concentration （their origin is in the natural geological environment）. Therefore, water treatment plant is in operation and its modernization is proposed. Furthermore, the paper deals with spring area intensification construction of a new hydrologic borehole, and managed and unmanaged infiltration of surface water. The proposal of protection zones revision consists of reduction to a 2nd level protection zone.
文摘The aim of the research was to evaluate overall quality of introduction of hazard analysis and critical control points （HACCP） prerequisites and HACCP procedures in catering sector in Latvia to reveal common problems that can provoke food-borne infection outbreaks. Certain quantitative indicators were defined and analyzed to conclude about characteristic trends related to implementation of food safety legislation. The state＇s monitoring data on surveillance of catering establishments were used. Fulfillment of legislative requirements in about 6,000 catering establishments was evaluated during time period of 2004-2009. The results of investigation were obtained with help of statistical analyses using MS Excel computer program. The results of the research suggest that most of catering establishments （e.g., 83.6% during 2009） meet the hygiene requirements only partly. Non-conformities regarding HACCP-based self-control procedures were fixed during 49.2% of inspections. The research reveals characteristic shortcomings that are established from year to year, e.g., improper hygiene of premises （established during 30%-40% of inspections, depending on the year）, poor hygiene of technological equipment （established during 27%-37% of inspections）, poor personnel hygiene （established during 11%-22% of inspections）, inadequate competence of food service personnel （established during 10%-35% of inspections）, as well as non-observance of cleaning-disinfection procedures and technological parameters for food processing （established during 11%-25% of inspections, depending on the year）. Actual shortcomings can lead to both the direct and the indirect cross-contamination of food. On the base of the research, it was concluded that further detailed assessment of potential food safety risks is relevant to establish risk-based monitoring activities in catering sector.
文摘This research developed the technology which united ISO-GUM （International Organization of Standard-Guide to Express of Uncertainty in Measurement） and QE （Quality Engineer） strategically and employed both feature efficiently. Although to tow technology of purpose use is same, each has future. The purpose is scheduled to improve the accuracy as a result of the clinical examinations. The result of research was used for diagnosis by equivalent Quality Control （eqQC） of the same level when and where, that are needed for broader-based medical system of the time of IT-era. Since National Institute of Standard and Technology （NIST） is recommend use of complicated operation by ISO-GUM for uniting, realization required the newest high quality computer technology. The algorithm of the complexity system of operation is that Fast Fourier Transform （FFT）, Inverse Fast Fourier Transform （IFFT） and Multi-variance Analysis （MA） are needed in Fig. 4. The main new improving points are changed of ambiguous expression method of data, and the pursuit which the cause of worsens accuracy put into data. All data with an assurance reported the final result for patient or donor. Medical laboratory quality system service is offered essential in the laboratory to be correct result for health care by Good Laboratory Practices （GLP） useful ISO 15189. Quality Assurance （QA） is achieved though system reliability.
