Glucose transporter 4 (GLUT4) is responsible for insulin-stimulated glucose transporting into the insulin-sensitive fat and muscle cells. The dynamics of GLUT4 storage vesicles (GSVs) remains to be explored and it is ...Glucose transporter 4 (GLUT4) is responsible for insulin-stimulated glucose transporting into the insulin-sensitive fat and muscle cells. The dynamics of GLUT4 storage vesicles (GSVs) remains to be explored and it is unclear how GSVs are arranged based on their mobility. We examined this issue in 3T3-L1 cells via investigating the three-dimensional mobility of single GSV labeled with EGFP-fused GLUT4. A thin layer of cytosol right adjacent to the plasma membrane was illuminated and successively imaged at 5 Hz under a total internal reflection fluorescence microscope with a penetration depth of 136 nm. Employing single particle tracking, the three-dimensional subpixel displacement of single GSV was tracked at a spatial precision of 22 nm. Both the mean square displacement and the diffusion coefficient were calculated for each vesicle. Tracking results revealed that vesicles moved as if restricted within a cage that has a mean radius of 160 nm, suggesting the presence of some intracellular tethering matrix. By constructing the histogram of the diffusion coefficients of GSVs, we observed a smooth distribution instead of the existence of distinct groups. The result indicates that GSVs are dynamically retained in a continuous and wide range of mobility rather than into separate classes.展开更多
While traditional open vein harvest was related to postoperative wound complications, endoscopic vein harvest was developed to minimize the morbidity in the greater saphenous vein harvest procedure. In this study, the...While traditional open vein harvest was related to postoperative wound complications, endoscopic vein harvest was developed to minimize the morbidity in the greater saphenous vein harvest procedure. In this study, these two procedures were compared for postoperative wound healing and long-term graft patency. We reviewed all consecutive patients undergoing elective off-pump coronary artery bypass grafting from January 2004 to December 2005 and collected data regarding wound complications and coronary events. Wound complications included dehiscence, excessive discharge, edema, altered sensation, cellulitis, hema-toma, pain scale, and superficial and deep wound infection. Coronary events were defined as diagnosis of myocardial infarction during the first year's follow-up. A total of 392 patients were included in our series, among whom 44 were excluded from the study due to emergent operation, preoperative intra-aortic balloon pump support, or the greater saphenous vein varicose characteristic, 78 belonged to open vein harvest group, and 270 to endoscopic vein harvest group. Wound complications were significantly less in the endoscopic group (5.2%) compared to the open group (19.2%) (P=0.0002). There was no significant difference on preopera-tive risk factors, total operative time, or hospitalization days. During one-year follow-up, both the early and late graft patency rates were similar between the two groups. Endoscopic vein harvest is safe and effective, which carries less risk for wound complica-tions and is associated with better satisfaction and cosmetic result than the traditional greater saphenous vein harvest procedure. The endoscopic vein harvest also demonstrates a great long-term patency.展开更多
For the polarimetric synthetic aperture radar interferometry (PoIInSAR) processing, it is necessary to coregister all the images, including the coregistration of polarimetric SAR images and the coregistration of inter...For the polarimetric synthetic aperture radar interferometry (PoIInSAR) processing, it is necessary to coregister all the images, including the coregistration of polarimetric SAR images and the coregistration of interferometric SAR images. Otherwise, the performance of the estimated optimal interferograms will be deteriorated. A generalized scattering vector (GSV) model is proposed to execute the PoIInSAR optimal interferograms estimation. The generalized scattering vector is constructed by the Pauli scattering vectors of the processing pixel and the surrounding pixels. Even though there are coregistration errors, all the polarimetric information of the current processing pixel is entirely included in the generalized scattering vector. Therefore, the GSV-based method can automatically recover the optimal scattering mechanisms of the processing pixel with coregistration errors either in interferoemetric channels or polarimetric channels. Theoretical analysis and processing results of simulated PoISARPro data and real PALSAR data validate the effectiveness and correctness of the proposed method.展开更多
Poplar(PopulusL.)is one of the most widely distributed tree species planted in the plains of China and plays an important role in wood products and ecological services.Accurate estimation of poplar Growing Stock Volum...Poplar(PopulusL.)is one of the most widely distributed tree species planted in the plains of China and plays an important role in wood products and ecological services.Accurate estimation of poplar Growing Stock Volume(GSV)is crucial for better understanding the ecological functions and economic values in plain regions.However,the striped distribution feature of poplar forests in plain regions makes traditional grid-based GSV modeling methods highly uncertain.This research took Lixin County and Yongqiao District as case studies to examine the advantages of using object-based GSV modeling approach over the traditional grid-based approaches for poplar GSV estimation.The canopy height variables and density variables were extracted from airborne LIDAR-derived Canopy Height Model(CHM)data through different grid sizes and segmentation unit for constructing the poplar GSV estimation models using the linear regression.The results indicate that(1)Significantly linear relationships exist between GSV and height percentile variables;(2)The estimation accuracy in Lixin can be effectively improved by incorporating the CHM density variables into height variables,with the coefficient of determination(R^(2))increasing from 0.46 to 0.71 and Root Mean Square Error(RMSE)decreasing from 20.23 to 14.94 m^(3)/ha when a grid-based approach was implemented at grid size of 26 m by 26 m(plot size).However,CHM density variables have no effect on estimation modeling in Yongqiao district.The patch sizes and shapes considerably affect the selection of modeling variables and accuracy of modeling prediction;(3)The object-based mapping approach outperforms the grid-based approach in solving the mixed plot problem.This is especially valuable in the study areas with striped forest distribution.This study shows that differences in poplar stand structure affect the selection of modeling variables and GSV modeling performance,and an object-based modeling approach is recommended for GSV estimation in the plain areas.展开更多
Objective: The great saphenous vein (GSV) is commonly used as a conduit for grafting during CABG surgery, and open GSV harvesting (OVH), commonly used with long incision to expose the vein. However, endoscopic vein ha...Objective: The great saphenous vein (GSV) is commonly used as a conduit for grafting during CABG surgery, and open GSV harvesting (OVH), commonly used with long incision to expose the vein. However, endoscopic vein harvesting (EVH) is an alternative approach, utilizing specialized instruments and small incisions to harvest the vein. Methods: A retrospective analysis was conducted on a cohort of patients who underwent Coronary artery bypass graft (CABG) requiring great saphenous vein (GSV) harvesting which was done by EVH or OVH procedures. Demographic variables, including age and gender, were assessed for both groups. Intraoperative variables such as the number of grafts, cardiopulmonary bypass time, X clamp time, and type of procedure were analyzed. Postoperative variables, including infection and bleeding rates, were also evaluated. Results: The study included 30 patients each undergoing Coronary artery bypass graft (CABG) with need of great saphenous vein harvesting which was done by EVH and OVH. Demographic variables were well-matched between the two groups in terms of age, while a significant difference in gender distribution was observed. Obesity and smoking were more prevalent in the OVH group, and EVH was associated with a higher mean number of grafts compared to OVH. Conversion to an open technique occurred in a portion of the EVH cases, and infection rates did not significantly differ between the EVH and OVH groups. However, the incidence of postoperative bleeding was significantly higher in the EVH group. Conclusion: This study provides valuable insights into the demographic, intraoperative, and postoperative variables associated with EVH and OVH techniques. EVH demonstrated advantages in terms of reduced infection rates compared to OVH. However, the higher incidence of postoperative bleeding associated with EVH raises concerns about potential risks.展开更多
Insulin-responsive GLUT4(glucose transporter 4)translocation plays a major role in regulating glucose uptake in adipose tissue and muscle.Whether or not there is a specialized secretory GSV(GLUT4 storage vesicle)pool,...Insulin-responsive GLUT4(glucose transporter 4)translocation plays a major role in regulating glucose uptake in adipose tissue and muscle.Whether or not there is a specialized secretory GSV(GLUT4 storage vesicle)pool,and more importantly how GSVs are translocated to the PM(plasma membrane)under insulin stimulation is still under debate.In the present study,we systematically analyzed the dynamics of a large number of single GLUT4-containing vesicles in 3T3-L1 adipocytes by TIRFM(total internal reflection fluorescence microscopy).We found that GLUT4-containing vesicles can be classified into three groups according to their mobility,namely vertical,stable,and lateral GLUT4-containing vesicles.Among these groups,vertical GLUT4-containing vesicles exclude transferrin receptors and move towards the PM specifically in response to insulin stimulation,while stable and lateral GLUT4-containing vesicles contain transferrin receptors and show no insulin responsiveness.These data demonstrate that vertical GLUT4-containing vesicles correspond to specialized secretory GSVs,which approach the PM directly and bypass the constitutive recycling pathway.展开更多
文摘Glucose transporter 4 (GLUT4) is responsible for insulin-stimulated glucose transporting into the insulin-sensitive fat and muscle cells. The dynamics of GLUT4 storage vesicles (GSVs) remains to be explored and it is unclear how GSVs are arranged based on their mobility. We examined this issue in 3T3-L1 cells via investigating the three-dimensional mobility of single GSV labeled with EGFP-fused GLUT4. A thin layer of cytosol right adjacent to the plasma membrane was illuminated and successively imaged at 5 Hz under a total internal reflection fluorescence microscope with a penetration depth of 136 nm. Employing single particle tracking, the three-dimensional subpixel displacement of single GSV was tracked at a spatial precision of 22 nm. Both the mean square displacement and the diffusion coefficient were calculated for each vesicle. Tracking results revealed that vesicles moved as if restricted within a cage that has a mean radius of 160 nm, suggesting the presence of some intracellular tethering matrix. By constructing the histogram of the diffusion coefficients of GSVs, we observed a smooth distribution instead of the existence of distinct groups. The result indicates that GSVs are dynamically retained in a continuous and wide range of mobility rather than into separate classes.
文摘While traditional open vein harvest was related to postoperative wound complications, endoscopic vein harvest was developed to minimize the morbidity in the greater saphenous vein harvest procedure. In this study, these two procedures were compared for postoperative wound healing and long-term graft patency. We reviewed all consecutive patients undergoing elective off-pump coronary artery bypass grafting from January 2004 to December 2005 and collected data regarding wound complications and coronary events. Wound complications included dehiscence, excessive discharge, edema, altered sensation, cellulitis, hema-toma, pain scale, and superficial and deep wound infection. Coronary events were defined as diagnosis of myocardial infarction during the first year's follow-up. A total of 392 patients were included in our series, among whom 44 were excluded from the study due to emergent operation, preoperative intra-aortic balloon pump support, or the greater saphenous vein varicose characteristic, 78 belonged to open vein harvest group, and 270 to endoscopic vein harvest group. Wound complications were significantly less in the endoscopic group (5.2%) compared to the open group (19.2%) (P=0.0002). There was no significant difference on preopera-tive risk factors, total operative time, or hospitalization days. During one-year follow-up, both the early and late graft patency rates were similar between the two groups. Endoscopic vein harvest is safe and effective, which carries less risk for wound complica-tions and is associated with better satisfaction and cosmetic result than the traditional greater saphenous vein harvest procedure. The endoscopic vein harvest also demonstrates a great long-term patency.
基金supported by the National Natural Science Foundation of China(6147127661671355)the Areospace T.T.&.C.Innovation Program
文摘For the polarimetric synthetic aperture radar interferometry (PoIInSAR) processing, it is necessary to coregister all the images, including the coregistration of polarimetric SAR images and the coregistration of interferometric SAR images. Otherwise, the performance of the estimated optimal interferograms will be deteriorated. A generalized scattering vector (GSV) model is proposed to execute the PoIInSAR optimal interferograms estimation. The generalized scattering vector is constructed by the Pauli scattering vectors of the processing pixel and the surrounding pixels. Even though there are coregistration errors, all the polarimetric information of the current processing pixel is entirely included in the generalized scattering vector. Therefore, the GSV-based method can automatically recover the optimal scattering mechanisms of the processing pixel with coregistration errors either in interferoemetric channels or polarimetric channels. Theoretical analysis and processing results of simulated PoISARPro data and real PALSAR data validate the effectiveness and correctness of the proposed method.
基金supported by the National Natural Science Foundation of China[grant number 32171787]National Key R&D Program of China[grant number 2021YFD2200400102].
文摘Poplar(PopulusL.)is one of the most widely distributed tree species planted in the plains of China and plays an important role in wood products and ecological services.Accurate estimation of poplar Growing Stock Volume(GSV)is crucial for better understanding the ecological functions and economic values in plain regions.However,the striped distribution feature of poplar forests in plain regions makes traditional grid-based GSV modeling methods highly uncertain.This research took Lixin County and Yongqiao District as case studies to examine the advantages of using object-based GSV modeling approach over the traditional grid-based approaches for poplar GSV estimation.The canopy height variables and density variables were extracted from airborne LIDAR-derived Canopy Height Model(CHM)data through different grid sizes and segmentation unit for constructing the poplar GSV estimation models using the linear regression.The results indicate that(1)Significantly linear relationships exist between GSV and height percentile variables;(2)The estimation accuracy in Lixin can be effectively improved by incorporating the CHM density variables into height variables,with the coefficient of determination(R^(2))increasing from 0.46 to 0.71 and Root Mean Square Error(RMSE)decreasing from 20.23 to 14.94 m^(3)/ha when a grid-based approach was implemented at grid size of 26 m by 26 m(plot size).However,CHM density variables have no effect on estimation modeling in Yongqiao district.The patch sizes and shapes considerably affect the selection of modeling variables and accuracy of modeling prediction;(3)The object-based mapping approach outperforms the grid-based approach in solving the mixed plot problem.This is especially valuable in the study areas with striped forest distribution.This study shows that differences in poplar stand structure affect the selection of modeling variables and GSV modeling performance,and an object-based modeling approach is recommended for GSV estimation in the plain areas.
文摘Objective: The great saphenous vein (GSV) is commonly used as a conduit for grafting during CABG surgery, and open GSV harvesting (OVH), commonly used with long incision to expose the vein. However, endoscopic vein harvesting (EVH) is an alternative approach, utilizing specialized instruments and small incisions to harvest the vein. Methods: A retrospective analysis was conducted on a cohort of patients who underwent Coronary artery bypass graft (CABG) requiring great saphenous vein (GSV) harvesting which was done by EVH or OVH procedures. Demographic variables, including age and gender, were assessed for both groups. Intraoperative variables such as the number of grafts, cardiopulmonary bypass time, X clamp time, and type of procedure were analyzed. Postoperative variables, including infection and bleeding rates, were also evaluated. Results: The study included 30 patients each undergoing Coronary artery bypass graft (CABG) with need of great saphenous vein harvesting which was done by EVH and OVH. Demographic variables were well-matched between the two groups in terms of age, while a significant difference in gender distribution was observed. Obesity and smoking were more prevalent in the OVH group, and EVH was associated with a higher mean number of grafts compared to OVH. Conversion to an open technique occurred in a portion of the EVH cases, and infection rates did not significantly differ between the EVH and OVH groups. However, the incidence of postoperative bleeding was significantly higher in the EVH group. Conclusion: This study provides valuable insights into the demographic, intraoperative, and postoperative variables associated with EVH and OVH techniques. EVH demonstrated advantages in terms of reduced infection rates compared to OVH. However, the higher incidence of postoperative bleeding associated with EVH raises concerns about potential risks.
基金Supported by the National Natural Science Foundation of China(Grant Nos.30470448 and 30130230)the National key Basic Research Program of China(Grant No.2004CB720000)+1 种基金the Knowledge Innovative Program of The Chinese Academy of Sciences(Grant Nos.KSCX2-SW-224 and Y2004018)the Li Foundation and the Sinogerman Scientific Center
文摘Insulin-responsive GLUT4(glucose transporter 4)translocation plays a major role in regulating glucose uptake in adipose tissue and muscle.Whether or not there is a specialized secretory GSV(GLUT4 storage vesicle)pool,and more importantly how GSVs are translocated to the PM(plasma membrane)under insulin stimulation is still under debate.In the present study,we systematically analyzed the dynamics of a large number of single GLUT4-containing vesicles in 3T3-L1 adipocytes by TIRFM(total internal reflection fluorescence microscopy).We found that GLUT4-containing vesicles can be classified into three groups according to their mobility,namely vertical,stable,and lateral GLUT4-containing vesicles.Among these groups,vertical GLUT4-containing vesicles exclude transferrin receptors and move towards the PM specifically in response to insulin stimulation,while stable and lateral GLUT4-containing vesicles contain transferrin receptors and show no insulin responsiveness.These data demonstrate that vertical GLUT4-containing vesicles correspond to specialized secretory GSVs,which approach the PM directly and bypass the constitutive recycling pathway.
