The objectives of this study were to optimize instrumental parameters and conditions for analysis of selected organop- hosphorus pesticides (OPPs) by gas chromatography (GC) with nitrogen-phosphorus detection (NPD) (G...The objectives of this study were to optimize instrumental parameters and conditions for analysis of selected organop- hosphorus pesticides (OPPs) by gas chromatography (GC) with nitrogen-phosphorus detection (NPD) (GC-NPD); to select an appropriate solvent system; to conduct a comparison of sonication and shaking extractions; and to select an appropriate procedure for extracting organophosphorus pesticides from soils. Procedure I consisted of n-hexane or petroleum ether together with acetone used as solvents, while Procedure contained several solvents including acetone, methanol, dichloromethane, and n-hexane or petroleum ether. Experimental results indicated that a mixture of petroleum ether/acetone (2:1, v/v) could be used in place of n-hexane/acetone (2:1, v/v) as it was a less expensive solvent system. In addition, shaking under a water bath at 20 °C was more effective than sonication. Also, Procedure I was more effective, safer, and more timesaving than Procedure Procedure I was applied to three soil types of different organic matter content, with recoveries of the OPPs from the yellow-brown soils, which had a higher organic matter content, being lower than those from the yellow and red soils.展开更多
[ Objective] The paper was to establish a quick detection method for residue of organphosphoras pesticides in chewing cane juices and provide guarantee for edible safety of chewing juice. [ Method] By using QuEChERS a...[ Objective] The paper was to establish a quick detection method for residue of organphosphoras pesticides in chewing cane juices and provide guarantee for edible safety of chewing juice. [ Method] By using QuEChERS and GC-NPD method, a rapid analysis method for residue of six kinds of organphosphorus pesti- cides （dichlorves, chlorpyrifos-methyl, malathion, fenthion, chlorpyrifos and triazophes） in sugarcane juices was established. [ Resdt] QuEChERS method was significantly superior to traditional extraction method and could extract six kinds of organphesphorus pesticides from chewing cane juices effectively, which gready saved organic reagent and time. Good linearity was found in different standard curves; when the concentration of pesticides was from 0. 020 toO. 100 rag/L, the re- covery rate was from 68.58% to 92.85%, and relative standard deviation （RSD） was ranged from 2.16% to 9.89%. [ Conclusion] The sensitivity, accuracy and precision of QuEChERS method were consistent with the technical requirements of pesticide residue determination. It not only could shorten sample processing cycle effectively, but also ensure that no impurity was in the sample peak and the recovery rate was also consistent with the requirements of residual analysis.展开更多
基金1Project supported by the Outstanding Youth Fund of the National Natural Science Foundation of China (No. 40325001),the National Key Basic Research Support Foundation of China (Nos. 2002CB415203 and 2002CB410805) and the Knowl-edge Innovation Program of
文摘The objectives of this study were to optimize instrumental parameters and conditions for analysis of selected organop- hosphorus pesticides (OPPs) by gas chromatography (GC) with nitrogen-phosphorus detection (NPD) (GC-NPD); to select an appropriate solvent system; to conduct a comparison of sonication and shaking extractions; and to select an appropriate procedure for extracting organophosphorus pesticides from soils. Procedure I consisted of n-hexane or petroleum ether together with acetone used as solvents, while Procedure contained several solvents including acetone, methanol, dichloromethane, and n-hexane or petroleum ether. Experimental results indicated that a mixture of petroleum ether/acetone (2:1, v/v) could be used in place of n-hexane/acetone (2:1, v/v) as it was a less expensive solvent system. In addition, shaking under a water bath at 20 °C was more effective than sonication. Also, Procedure I was more effective, safer, and more timesaving than Procedure Procedure I was applied to three soil types of different organic matter content, with recoveries of the OPPs from the yellow-brown soils, which had a higher organic matter content, being lower than those from the yellow and red soils.
基金Supported by Basic Scientific Research Special Project of Guangxi Academy of Agricultural Sciences(GNK2012YM11)Scientific Research and Technology Development Program of Nanning City(201102026B)+1 种基金Fundamental Research Project of Guangxi Academy of Agricultural Sciences(G2009001,201001G)Science and Technology Development Fund Project of Guangxi Academy of Agricultural Sciences(201002Z)
文摘[ Objective] The paper was to establish a quick detection method for residue of organphosphoras pesticides in chewing cane juices and provide guarantee for edible safety of chewing juice. [ Method] By using QuEChERS and GC-NPD method, a rapid analysis method for residue of six kinds of organphosphorus pesti- cides （dichlorves, chlorpyrifos-methyl, malathion, fenthion, chlorpyrifos and triazophes） in sugarcane juices was established. [ Resdt] QuEChERS method was significantly superior to traditional extraction method and could extract six kinds of organphesphorus pesticides from chewing cane juices effectively, which gready saved organic reagent and time. Good linearity was found in different standard curves; when the concentration of pesticides was from 0. 020 toO. 100 rag/L, the re- covery rate was from 68.58% to 92.85%, and relative standard deviation （RSD） was ranged from 2.16% to 9.89%. [ Conclusion] The sensitivity, accuracy and precision of QuEChERS method were consistent with the technical requirements of pesticide residue determination. It not only could shorten sample processing cycle effectively, but also ensure that no impurity was in the sample peak and the recovery rate was also consistent with the requirements of residual analysis.
