Background:Polycystic ovary syndrome(PCOS)is one of the most common reproductive endocrine metabolic diseases.Combined use of metformin and diane-35 has better curative effect in regulating serum hormone level(LH,FSH,...Background:Polycystic ovary syndrome(PCOS)is one of the most common reproductive endocrine metabolic diseases.Combined use of metformin and diane-35 has better curative effect in regulating serum hormone level(LH,FSH,T and E2)than using metformin alone.Traditional Chinese medicine(TCM)can also be used to treat PCOS.According to some studies,the combined use of metformin and diane-35 and TCM have achieved better curative effect than combining metformin and diane-35 in the treatment of patients with PCOS.Methods:Computerized searches of the science,Medline,VIP,Wan Fang and China HowNet(CNKI)databases were conducted to identify eligible randomized controlled trials(RCTs)from the data obtained up to March 1,2022.The Cochrane Collaboration risk of bias tool was used to assess the risk of bias in individual RCTs,and R software(version 4.0.3)was used for data statistical analysis.Results:Nine RCTs involving 1035 patients were included.Comparing to D+M,significant reduce of LH(mean difference[MD]:-1.93,95%confidence interval[CI]:-3.44,-0.42;Unit:U/L P<0.01;I2=89%)、T(MD:-1.44,95%CI-2.59,-0.30;Unit:nmol/L P<0.01;I2=98%)and significant increase of E2(MD:31.43,95%CI 24.54,38.33;Unit:pmol/L P<0.01;I2=96%)were shown in TCM+D+M.Comparing to D+M,TCM+D+M group has higher ovulation rate(RR 1.1495%CI 1.07,1.22;P=0.42;I2=0%)and higher pregnancy rate(RR 1.2995%CI 1.15,1.44;P=0.37;I2=7%).There is no significant difference between the two therapies in FSH changes(MD:-1.00,95%CI-2.27,0.28;Unit:U/L P<0.01;I2=95%).Subgroup analysis showed that compared with the Guizhi Fuling capsule group,the Kuntai capsule group had more FSH reduction and E2 increase more.In other outcome indicators,the two subgroup did not show significant differences.Conclusion:Kuntai Capsule+Diane-35+Metformin is better than Guizhi Fuling Capsule in reducing FSH,and it is also better in increasing E2.There was no significant difference between the two in LH and T hormones.There was no significant difference between Kuntai Capsules+Diane-35+Metformin and Guizhi Fuling Capsules+Diane-35+Metformin.As for the effect in lessen insulin resistance,Kuntai Capsule+Diane-35+Metformin was significantly better than Guizhi Fuling Capsules+Diane-35+Metformin.展开更多
The core sampling experiments were conducted after hydraulic fracturing in the three-dimensional development zone of Fuling shale gas.Six coring wells of different well types were systematically designed.Based on the ...The core sampling experiments were conducted after hydraulic fracturing in the three-dimensional development zone of Fuling shale gas.Six coring wells of different well types were systematically designed.Based on the integrated engineering technology of post-fracturing drilling,coring and monitoring of shale and the analysis of fracture source tracing,the evaluation of the fracture network after fracturing in the three-dimensional development of shale gas was conducted.The data of core fractures after fracturing indicate that three major types of fractures are formed after fracturing:natural fractures,hydraulic fractures,and fractures induced by external mechanical force,which are further classified into six subcategories:natural structural fractures,natural bedding fractures,hydraulic fractures,hydraulically activated fractures,drilling induced fractures,and fractures induced by core transportation.The forms of the artificial fracture network after fracturing are complex.Hydraulic fractures and hydraulically activated fractures interweave with each other,presenting eight forms of artificial fracture networks,among which the“一”-shaped fracture is the most common,accounting for approximately 70%of the total fractures.When the distance to the fractured wellbore is less than 35 m,the density of the artificial fracture network is relatively high;when it is 35–100 m,the density is lower;and when it is beyond 100 m,the density gradually increases.The results of the fracture tracing in the core sampling area confirm that the current fracturing technology can essentially achieve the differential transformation of the reservoir in the main area of Jiaoshiba block in Fuling.The three-layer three-dimensional development model can efficiently utilize shale gas reserves,although there is still room for improvement in the complexity and propagation uniformity of fractures.It is necessary to further optimize technologies such as close-cutting combined with temporary blocking and deflection within fractures or at fracture mouths,as well as limited flow perforation techniques,to promote the balanced initiation and extension of fractures.展开更多
Comprehensive quantitative evaluation of shale gas content and the controlling factors in different occurrence states is of great significance for accurately assessing gas-bearing capacity and providing effective well...Comprehensive quantitative evaluation of shale gas content and the controlling factors in different occurrence states is of great significance for accurately assessing gas-bearing capacity and providing effective well-production strategies. A total of 122 core samples from well JY-A in the Fuling shale gas field were studied to reveal the characteristics of S_1 l shale,15 of which were selected to further predict the shale gas content in different occurrence states, which are dependent on geological factors in the thermal evolution process. Geological parameters were researched by a number of laboratory programs, and the factors influential in controlling shale gas content were extracted by both PCA and GRA methods and prediction models were confirmed by the BE method using SPSS software. Results reveal that the adsorbed gas content is mainly controlled by TOC, Ro, SSA, PD and pyrite content, and the free gas content is mainly controlled by S_2, quartz content, gas saturation and formation pressure for S_1 l in well JY-A. Three methods, including the on-site gas desorption method, the empirical formula method, and the multiple regression analysis method were used in combination to evaluate the shale gas capacity of well JY-A, all of which show that the overall shale gas content of well JY-A is in the range of 2.0–5.0 m^3/t and that the free gas ratio is about 50%, lower than that of well JY-1. Cause analysis further confirms the tectonics and preservation conditions of S_1 l in the geological processes, especially the influence of eastern boundary faults on well JY-A, as the fundamental reasons for the differences in shale gas enrichment in the Jiaoshiba area.展开更多
Guizhi Fuling capsule(GFC), a traditional Chinese medicine(TCM) with effects of promoting blood circulation and dissipating blood stasis, has been widely used in the clinic. Because of the complex matrix and various c...Guizhi Fuling capsule(GFC), a traditional Chinese medicine(TCM) with effects of promoting blood circulation and dissipating blood stasis, has been widely used in the clinic. Because of the complex matrix and various chemical structure types, quality control of GFC remains great challenge. In the present study, an ultra performance liquid chromatography hybrid triple-quadrupole mass spectrometry(UPLC-QQQ MS) method with ultrafast positive/negative ionization switching was developed for simultaneous determination of 18 bioactive components in GFC, including methyl gallate, ethyl gallate, oxypaeoniflorin, benzoic acid, albiflorin, paeonolide, paeoniflorin, 1, 2, 3, 4, 6-pentagalloylglucose, mudanpioside C, benzoyloxypaeoniflorin, benzoylpaeoniflorin, pachymic acid, amygdalin, cinnamaldehyde, paeonol, cinnamic acid, 4-hydroxybenzoic acid, and gallic acid. Separation was performed on an Agilent Zorbax Extend–C18 column(2.1 mm × 50 mm, 1.8 μm), using a gradient elution with acetonitrile and water containing 0.1% formic acid. Cholic acid was selected as the internal standard. This newly developed method was fully validated for linearity, precision, accuracy, and stability, and then applied to quality assessment of GFC. Finally, the batch-to-batch reproducibility of GFC samples was evaluated by the cosine ration and Euclidean distance method, which showed high quality consistency. The results demonstrated that the developed method provided a reasonable and powerful manner for quality control of GFC.展开更多
In the Jiaoshiba block of the Fuling shale gas field,the employed reserves and recovery factor by primary well pattern are low,no obvious barrier is found in the development layer series,and layered development is dif...In the Jiaoshiba block of the Fuling shale gas field,the employed reserves and recovery factor by primary well pattern are low,no obvious barrier is found in the development layer series,and layered development is difficult.Based on the understanding of the main factors controlling shale gas enrichment and high production,the theory and technology of shale gas three-dimensional development,such as fine description and modeling of shale gas reservoir,optimization of three-dimensional development strategy,highly efficient drilling with dense well pattern,precision fracturing and real-time control,are discussed.Three-dimensional development refers to the application of optimal and fast drilling and volume fracturing technologies,depending upon the sedimentary characteristics,reservoir characteristics and sweet spot distribution of shale gas,to form"artificial gas reservoir"in a multidimensional space,so as to maximize the employed reserves,recovery factor and yield rate of shale gas development.In the research on shale gas three-dimensional development,the geological+engineering sweet spot description is fundamental,the collaborative optimization of natural fractures and artificial fractures is critical,and the improvement of speed and efficiency in drilling and fracturing engineering is the guarantee.Through the implementation of three-dimensional development,the overall recovery factor in the Jiaoshiba block has increased from 12.6%to 23.3%,providing an important support for the continuous and stable production of the Fuling shale gas field.展开更多
One of the important indicators of shale gas reservoir excavation is capacity evaluation,which directly affects whether large-scale shale gas reservoirs can be excavated.Capacity evaluation is the basis of system anal...One of the important indicators of shale gas reservoir excavation is capacity evaluation,which directly affects whether large-scale shale gas reservoirs can be excavated.Capacity evaluation is the basis of system analysis and dynamic prediction.Therefore,it is particularly important to conduct capacity evaluation studies on shale gas horizontal wells.In order to accurately evaluate the horizontal well productivity of shale gas staged fracturing,this paper uses a new method to evaluate the productivity of Fuling shale gas.The new method is aimed at the dynamic difference of horizontal wells and effectively analyzes the massive data,which are factors affecting the productivity of shale gas horizontal wells.According to the pressure system,production dynamic characteristics,well trajectory position,fracturing transformation mode and penetration depth,32 wells were divided into four types.Then,based on the classification,the principal component analysis methods can be used to evaluate the horizontal well productivity of shale gas.The new method of capacity evaluation has improved the accuracy by 10.25%compared with the traditional method,which provides a theoretical basis for guiding the efficient development of the horizontal wells of Fuling shale gas.展开更多
The Fuling shale gas field in China is the largest shale gas field as well as the largest of its type discovered in any Lower Paleozoic formation.In this study,the geology and production of the upper and lower gas lay...The Fuling shale gas field in China is the largest shale gas field as well as the largest of its type discovered in any Lower Paleozoic formation.In this study,the geology and production of the upper and lower gas layers in the Fuling shale gas field are evaluated in terms of structure,shale quality,fault,initial production,and estimated ultimate recovery(EUR).The shale in the lower gas layer of the Jiaoshiba anticline is a high-quality reservoir,where the space is dominated by organic pores in kerogen,and the gas content is high.The shale gas wells reveal relatively high initial production and EUR.However,the shale in the upper gas layer of the Jiaoshiba anticline has reservoir space mainly composed of clay mineral pores and organic pores within bitumen,and the gas content is low.In terms of structure,primary gas migration may occur in the upper gas layer,resulting in free gas accumulation in the structural high,where the development effects are generally better than those in the structural low.The lower gas layer in the Pingqiao anticline,is the main interval for shale gas accumulation and development due to the high-quality shale.Under the influence of faults,the efficiency of exploration wells emplaced on top of the anticline is much lower a compared with those in the flanks.The residual synclines close to the Sichuan Basin,including the Baima and Baitao anticlines,are characterized by more recent uplifts,larger area,greater distance from the deep and large faults,and early fracture closure.Therefore,we recommend that the shale gas exploration and development should be carried out preferentially in areas close to the center of the residual synclines,featuring relatively high-pressure coefficient and moderate burial depth.展开更多
Geological characteristics,geomechanical behavior and hydraulic fracture propagation mechanism in the Marcellus shale gas play are analyzed and compared with China’s Fuling shale play.Successful experiences in hydrau...Geological characteristics,geomechanical behavior and hydraulic fracture propagation mechanism in the Marcellus shale gas play are analyzed and compared with China’s Fuling shale play.Successful experiences in hydraulic fracturing and shale gas development in the Marcellus shale gas play are summarized,which might be applicable in other shale plays.The main factors contributing to the successful development of the Marcellus shale gas play include adoption of advanced drilling and completion technologies,increases of hydraulic fracturing stages,proppant concentration and fluid injection volume.The geological and geomechanical mechanisms related to those technologies are analyzed,particularly the in-situ stress impacts on hydraulic fracturing.The minimum horizontal stress controls where the fractures are initiated,and the maximum horizontal stress dominates the direction of the hydraulic fracture propagation.Hydraulic fracturing performed in the shale reservoir normally has no stress barriers in most cases because the shale has a high minimum horizontal stress,inducing hydraulic fractures propagating beyond the reservoir zone,resulting in inefficient stimulation.This is a common problem in shale plays,and its mechanism is studied in the paper.It is also found that the on-azimuth well has a higher productivity than the off-azimuth well,because shear fractures are created in the off-azimuth well,causing main fractures to kink and increasing fracture tortuosity and friction.The Fuling shale gas play has a markedly higher minimum horizontal stress and much smaller horizontal stress difference.The high minimum horizontal stress causes a much higher formation breakdown pressure;therefore,hydraulic fracturing in the Fuling shale gas play needs a higher treatment pressure,which implies higher difficulty in fracture propagation.The small difference in the two horizontal stresses in the Fuling shale gas play generates shorter and more complex hydraulic fractures,because hydraulic fractures in this case are prone to curve to preexisting fractures.To overcome these difficulties,we recommend reducing well spacing and increasing proppant concentration to increase gas productivity for the Fuling shale gas development.展开更多
OBJECTIVE:To observe and explore the effect of Fuling(Poria) in alleviating the spleen deficiency symptom pattern(SDSP).METHODS:We established an animal model of SDS in Sprague-Dawley(SD) rats by treating them with de...OBJECTIVE:To observe and explore the effect of Fuling(Poria) in alleviating the spleen deficiency symptom pattern(SDSP).METHODS:We established an animal model of SDS in Sprague-Dawley(SD) rats by treating them with deficiency-inducing factors,including irregular feeding and tail clamping.Mice were administered Fuling(Poria) and its extracts(raw/cooked powder,aqueous/alcohol extract) by gavage once a day for 21 d.The body weight,rectal temperature,and spleen and thymus organ coefficients were calculated.The levels of motilin(MTL),gastrin(GAS),aquaporin 2(AQP2),interleukin 2(IL-2),IL-4,and 5-hydroxytryptamine(5-HT) in the serum and the level of AQP2 in the kidneys were evaluated by enzyme-linked immunosorbent assay.RESULTS:Fuling(Poria) and its extracts did not change the body weight,rectal temperature,and organ coefficients of the spleen and thymus.However,it reduced the levels of MTL and GAS and increased the levels of IL-2 and AQP2.In addition,the levels of IL-4 and 5-HT showed no significant alteration.CONCLUSIONS:These results suggested the crucial function of Fuling(Poria) in SDSP,especially promoting digestive function and water metabolism.展开更多
Marine shale gas in South China is widely distributed and demonstrates an enriched resource. Compared with the North American commercial shale gas field, the shale formation in South China is featured by old age, mult...Marine shale gas in South China is widely distributed and demonstrates an enriched resource. Compared with the North American commercial shale gas field, the shale formation in South China is featured by old age, multiple stages tectonic movements, and high thermal evolution degree and complex reservoir forming conditions. As a result, the existing theories and technical methods of exploration in North America cannot be simply applied to South China. Since 2007, based on the in-depth study on a large quantity of analytical test data, we have conducted the theory and technology research for the southern marine shale gas, found Wufeng-Longmaxi Group deep water shelf high-quality shale gas critical parameters coupling laws, proposed the “Binary Enrichment” theory for highly evolved marine shale gas in southern complex tectonic zone, and established a zone selection and evaluation criteria, which are based on the quality of shale, for the key of preservation condition, and for the purpose of economy. Taking this as a guide, we selected Longmaxi formation Lower Silurian of Fuling area in southeastern Sichuan Province as the preferred breakthrough field for the shale gas exploration. The Jiaoye 1 well has the daily gas production of 20.3 × 10<sup>4</sup> m<sup>3</sup>, which embarked the found of China’s first large-scale business development gas field, the Fuling shale gas field. And we submit the first domestic shale gas proven reserves of 1067.5 × 10<sup>8</sup> m<sup>3</sup>. The Fuling shale gas reservoirs are deep water shelf marine high-quality shale, with favorable thickness and even distribution, without dissection in the middle. They are typical self-generation and self-storage shale gas reservoirs. The gas fields have high production gas well;high pressure gas reservoir, good gas components and good exploit results, and there for the Fuling shale gas field is characterized by mid-depth, high pressure, high reservoir pressure, and high quality gas accumulation. Up to March 24, 2014, all of the 101 fracture-gas testing wells have obtained middle and upper shale airflow, with the average single well test production of 32.6 × 10<sup>4</sup> m<sup>3</sup>/d. In the exploration and development process, technology series in geological evaluation, horizontal well, drilling, well completion, piecewise fracturing techniques have been gradually formed. It is of great importance to commercially develop the domestic shale gas and promote the restructuring of China’s energy structure.展开更多
For the sake of figuring out the influential mechanisms of structural characteristics on the productivity of shale gas wells,the structural characteristics of the Jiaoshiba Block in the Fuling shale gasfield,Sichuan B...For the sake of figuring out the influential mechanisms of structural characteristics on the productivity of shale gas wells,the structural characteristics of the Jiaoshiba Block in the Fuling shale gasfield,Sichuan Basin,were analyzed.Then,based on well test data of more than 190 horizontal wells,the effects of structures on shale gas well productivity were discussed systematically,and the main structural factors of different structural units in the Jiaoshiba Block that influence the productivity of shale gas wells were clarified.The following results were obtained.First,the structural units in the Jiaoshiba Block were obviously different in structural characteristics and their deformation strength is different.Second,the influence of structural characteristics on shale gas well productivity is directly manifested in gas-bearing property and fracturing effect.The stronger the structural deformation and the more developed the large faults and natural fractures,the more easily shale gas escapes and the poorer the gas bearing property will be,and vice versa.Third,The stronger the structural deformation,the more developed the fractures,the greater the burial depth and the higher the compressive stress of negative structures,the worse the fracturing effect will be,and vice versa.And fourth,Tectonics is the key factor controlling the difference of shale gas productivity between different structural units in the Jiaoshiba Block,but the main structural factors influencing the productivity are different in different structural units.展开更多
Enhancing Schwann cell proliferation may be beneficial for peripheral nerve repair and nerve regeneration. A traditional herbal formula composed of Fuling （poria cocos）, Baizhu （Atractylodes macrocephala）, and Dan...Enhancing Schwann cell proliferation may be beneficial for peripheral nerve repair and nerve regeneration. A traditional herbal formula composed of Fuling （poria cocos）, Baizhu （Atractylodes macrocephala）, and Danggui （Angelica sinensis） （FBD） improves neuronal survival and growth, and FBD may promote the secretion of brain-derived neurotrophic factor. However, the mechanism underlying Schwann cell proliferation remains unclear. We tested whether FBD enhanced the proliferation of human Schwann cells. FBD （20 ug/mL） increased Schwann cell viability and survival and increased the number of cells at G2/M and S phases. FBD also increased nerve growth factor and brain-derived neurotrophic factor expression in Schwann cells, with maximum efficacy at 20 ug/mL.展开更多
Carbonate rocks in the first member of the Middle Permian Maokou Formation in the Sichuan Basin(hereinafter“Mao 1 Member”for short)have been taken as a set of carbonate source rocks,and they have not been specifical...Carbonate rocks in the first member of the Middle Permian Maokou Formation in the Sichuan Basin(hereinafter“Mao 1 Member”for short)have been taken as a set of carbonate source rocks,and they have not been specifically studied from the aspects of reservoir evaluation and testing.By referring the exploration ideas of unconventional natural gas,the Mao 1 Member in Fuling area of southeastern Sichuan Basin has obtained industrial gas flow in development wells in recent years.In order to further clarify the natural gas exploration potential of the Mao 1 Member in this area,it is necessary to study its sedimentary characteristics,natural gas accumulation conditions and main control factors based on the data of field section measurement,systematic coring in drilling and laboratory testing.The following research results were obtained.First,the gas reservoir in the Mao 1 Member in the Fuling area is of sourceereservoir integration,and its natural gas ismainly enriched in blackish graymarlite and nodularmarlite.Second,its reservoir spaces are dominated by grainmargin pores(fractures),diagenetic shrinkage pores(fractures),organic pores and fractures.Third,the pores aremostly in nanometer scale,and the main pore diameter is in the range of 5-50 nm,which is between shale reservoir and conventional reservoir,with strong heterogeneity.Fourth,the gas reservoir is characterized by source-reservoir coexistence,lithology controlling reservoir and extensive layered distribution,presenting two-stage differential hydrocarbon enrichment,namely intraformational near-source enrichment in the early stage and interformational blowdown adjustment in the late stage.Fifth,the development of blackish gray organic-rich fine marlite which is deposited with the episodic upwelling in the outer ramp facies belt is the foundation for the natural gas accumulation,the transformation of clay minerals controls the development of higher-quality reservoirs,good preservation conditions are the key to the natural gas accumulation,and fracture development is favorable for the enrichment and high yield of natural gas.In conclusion,theMao 1Member in this area is a special type of gas reservoir,i.e.,carbonate source rock gas reservoir,which has greater potential of natural gas exploration and industrial gas flow have been obtained in several wells.The discovery of such type of gas reservoirs not only expands the domain of natural gas exploration in the Sichuan Basin,but provides the reference for the natural gas exploration in other areas of South China.展开更多
OBJECTIVE To evaluate the effect of Guizhi Fuling Capsule active pharmaceutical ingredient(API)and its fractions on human breast cancer cells proliferation by high-throughput screening assay.METHODS The crude fraction...OBJECTIVE To evaluate the effect of Guizhi Fuling Capsule active pharmaceutical ingredient(API)and its fractions on human breast cancer cells proliferation by high-throughput screening assay.METHODS The crude fractions were obtained from the extraction and elution of the API of Guizhi Fuling Capsule,and 929 standard fractions were obtained by the optimal separation conditions.Sulforhodamine B(SRB)method was used to evaluate the effects of the Guizhi Fuling capsule API and929 kinds of fractions on the proliferation of human breast cancer cells MCF-7 and MDA-MB-231.RESULTS The Guizhi Fuling capsule API had a strong ability to inhibit the proliferation of MCF-7 cells at high concentration and the ability to inhibit MDA-MB-231 cells' proliferate at low concentration following 72 h treatment;some samples of 929 fractions(5μg·mL^(-1))was found to have a breast cancer cell growth inhibition rate above 50%,without toxicity on HUVECs proliferation.CONCLUSION The API of Guizhi Fuling capsule had significant cytotoxicity effects on these two human breast cancer cells,with significant concentration-and time-dependent manner.展开更多
Objective:The mechanism of Chinese herbal medicine"Bi xie and Tu fuling"on the treatment of gouty arthritis was explored through network pharmacological methods.Methods:First,the TCMSP Chinese medicine syste...Objective:The mechanism of Chinese herbal medicine"Bi xie and Tu fuling"on the treatment of gouty arthritis was explored through network pharmacological methods.Methods:First,the TCMSP Chinese medicine system pharmacology database and analysis platform were used to define the bioavailability(OB)30%and drug-likeness(DL)0.18.Database to mine genetic targets related to gouty arthritis disease.Finally,the two genes are intersected to construct a Chinese medicine regulatory network of Chinese medicine-components-target genes-disease.The genes in the network are used to construct a protein interaction network to find core genes for analysis of GO and KEGG.Results:There were a total of 84 active ingredients in Chinese medicine Poria and Poria cocos,and 17 effective ingredients and 180 genetic targets were obtained after screening.600 disease targets for gouty arthritis were identified,and 58 were common target genes for both.PPI network analysis revealed that IL1β,VEGFA,MAPK1,IL10,and PTGS2 may be drugs for treating gouty arthritis.Key targets.GO enrichment analysis identified 1,399 entries(P<0.05),of which biological processes mainly include biological stimulation,biological regulation,cell metabolism,etc;KEGG pathway enrichment analysis identified 152 signal pathways,of which signal pathways involved inflammation,metabolism,In terms of aging,mainly including the IL-4,IL-10,IL-13,IL-17signal pathway,etc.Conclusion:"Bi xie and Tu fuling"drugs have anti-inflammatory and immunological effects on gouty arthritis through multiple targets and multiple pathways.The mechanism of lowering uric acid and protecting liver and kidney is not clear.It needs molecular biology research to improve it.The mechanism of the action of the"Bi xie and Tu fuling"medicine pair provides new ideas for the clinical prescriptions.展开更多
Objective:To explore the main active components and mechanism of Guizhi Fuling pill in the treatment of ovarian cancer based on network pharmacology and molecular docking technology.Methods:TCMSP database was used to ...Objective:To explore the main active components and mechanism of Guizhi Fuling pill in the treatment of ovarian cancer based on network pharmacology and molecular docking technology.Methods:TCMSP database was used to retrieve and screen the active components of each Chinese medicine in Guizhi Fuling pill.The main active components of the medicine were screened through the network analysis function of Cytoscape software,and the target of the main active components of the medicine was found in UniProt database.Using genecards,OMIM,digsee,TTD,and drugbank database to retrieve the related targets of ovarian cancer.The interaction between the target of the main active components of the drug and the related target of ovarian cancer was obtained by R software.The intersection targets are imported into string database for PPI analysis,and then the results are imported into Cytoscape to get the hub gene using the cyclohubba plug-in.KEGG pathway analysis and go enrichment analysis were carried out in metascape database.At last,autodock tools was used to verify the main active ingredients and the selected target proteins.PyMOL and LigPlus software visualized the results.Results:The main active components of Guizhi Fuling pills were quercetin,β-sitosterol,kaempferol,hederagenin,catechin,ellagic acid,stigmasterol and Douglas fir.There are 20 pivotal genes of drug effect on ovarian cancer,including VEGFA,AKT1,mapk8,Jun,MMP9,IL6,TNF,CXCL8,PTGS2,TP53,CASP3,mapk1,EGF,ESR1,EGFR,Myc,FOS,CCL2,CXCL8 and IL1B.According to KEGG pathway analysis,10 pathways with the highest correlation were found:cancer signaling pathway,MAPK signaling pathway,fluid shear stress and atherosclerosis,hepatitis B,trypanosomiasis,age-rage signaling pathway in diabetic complications,tumor necrosis factor signaling pathway,pertussis,IL-17 signaling pathway,and bladder cancer.Go enrichment analysis has screened out 10 important items in molecular biological function,cell components and biological process.The proteins encoded by IL6,mapk1,VEGFA,AKT1,TP53,TNF and CCL2 were used as targets to dock with the main active components of the drug respectively,and the best results were TNFαand ellagic acid.Conclusion:The main active components of Guizhi Fuling pill regulate multiple signal pathways by regulating IL6,VEGFA,CCL2,TNF,MMP9 and other cytokines,and inhibit the proliferation and metastasis of cancer cells.展开更多
Objective:To investigate the mechanism of action of Guizhi Fuling pill in the treatment of atherosclerosis with network pharmacology.Methods:The active components of Guizhi Fuling pill were screened by specific ADME f...Objective:To investigate the mechanism of action of Guizhi Fuling pill in the treatment of atherosclerosis with network pharmacology.Methods:The active components of Guizhi Fuling pill were screened by specific ADME features on Traditional Chinese Medicine Systems Pharmacology platform,and their targets were obtained.Targets of atherosclerosis were extracted and screened from GeneCards database,OMIM database,DrugBank database and DisGeNET database,and the intersection of component targets and disease targets were regarded as potential targets.The protein-protein interaction network of potential targets was constructed by STRING database and Cytoscape 3.7.2.GO analysis and KEGG pathway analysis of potential targets were performed by Metascape platform.The“drugs-componenst-targets-pathways”network was constructed by Cytoscape 3.7.2.SwissDock was used for molecular docking of active components and key targets.Results:The main components of Guizhi Fuling pill in the treatment of atherosclerosis were quercetin,beta-sitosterol,kaempfero,hederagenin,baicalein,etc.The key targets mainly included PGST2,RELA,AKT1,etc.Molecular docking analysis showed that the binding energy of the main active components to the core target was less than-7 kcal/mol.The associated pathways mainly included fluid shear stress and atherosclerosis,PI3K-Akt signaling pathway and AGE-RAGE signaling pathway.Conclusion:Through this study,we initially revealed the possible mechanism of Guizhi Fuling pill in treating atherosclerosis through anti-inflammatory,reducing blood lipids,and regulating endothelial function.展开更多
This paper reviews the research progress of Guizhi Fuling Pill in the clinical application of gynecology,such as the treatment of uterine leiomyoma,ovarian cyst,infertility and dysmenorrhea,in order to provide further...This paper reviews the research progress of Guizhi Fuling Pill in the clinical application of gynecology,such as the treatment of uterine leiomyoma,ovarian cyst,infertility and dysmenorrhea,in order to provide further research ideas for clinical researchers.展开更多
Objective:To investigate the effect of Guizhi Fuling Capsule on sex hormones and tumor markers in patients with uterine fibroids, and observe its clinical efficacy.Methods: A total of 120 patients with uterine fibroid...Objective:To investigate the effect of Guizhi Fuling Capsule on sex hormones and tumor markers in patients with uterine fibroids, and observe its clinical efficacy.Methods: A total of 120 patients with uterine fibroids who were treated in our hospital from August 2016 to August 2017 were selected according to the order of treatment and divided into observation group and control group, 60 cases each. Mifepristone was administered to the control group and mifepristone combined with Guizhi Fuling Capsules was administered to the observation group. The levels of hemoglobin, vascular endothelial growth factor (VEGF), sex hormones, tumor markers, and inflammatory factors before and after treatment were compared between the two groups.Result:The levels of hemoglobin, vascular endothelial growth factor (VEGF), sex hormones, tumor markers, and inflammatory factors before and after treatment were compared between the two groups. After treatment, hemoglobin levels increased significantly in the two groups, VEGF, sex hormones FSH, LH and E2, tumor markers HE4, CA125, inflammatory cytokines TNF-α and IFN-γ levels decreased significantly. The hemoglobin level of the observation group was (117.09±11.51) g/L higher than that of the control group , and the level of VEGF was (0.69±0.06) pg/mL lower than that of the control group. The levels of sex hormone FSH, LH and E2 in the observation group were (15.87±1.56) U/L, (10.69±1.06) U/L and (4.95±0.51) pmol/L lower than those of the control group. The levels of HE4, CA125, TNF-α and IFN-γ gamma in the observation group were (52.47±5.24) pmol/L, (15.46±1.53) U/mL, (40.41±4.05) and (16.72±1.62) pg/mL lower than those of the control group.Conclusion:Guizhi Fuling Capsule can significantly improve the level of sex hormones and tumor markers, relieve inflammatory stress and improve the therapeutic effect.展开更多
Objective To observe the effects of Guizhi Fuling capsule(GZFLC)on RPMI 8226 cells and explore the mechanisms.Methods Cell Counting Kit-8(CCK-8)assays and flow cytometry were used to detect the viability and apoptosis...Objective To observe the effects of Guizhi Fuling capsule(GZFLC)on RPMI 8226 cells and explore the mechanisms.Methods Cell Counting Kit-8(CCK-8)assays and flow cytometry were used to detect the viability and apoptosis levels of RPMI 8226 cells.The effects on mitochondria were examined by ROS and JC-1 assays.Western blotting was used to detect the expression of B cell lymphoma-2(Bcl-2),Bax,cleaved caspase-3,and Apoptotic protease-activating factor 1(Apaf-1).Results GZFLC drug serum decreased the viability and increased the apoptosis of RPMI 8226 cells.In addition,this drug increased ROS levels and decreased the mitochondrial membrane potential(MMP).Western blotting showed that the Bcl-2/Bax ratios were decreased in the GZFLC drug serum-treated groups,whereas the expression levels of cleaved caspase-3 and Apaf-1 were increased.Conclusion GZFLC promoted apoptosis of myeloma cells through the mitochondrial apoptosis pathway.展开更多
文摘Background:Polycystic ovary syndrome(PCOS)is one of the most common reproductive endocrine metabolic diseases.Combined use of metformin and diane-35 has better curative effect in regulating serum hormone level(LH,FSH,T and E2)than using metformin alone.Traditional Chinese medicine(TCM)can also be used to treat PCOS.According to some studies,the combined use of metformin and diane-35 and TCM have achieved better curative effect than combining metformin and diane-35 in the treatment of patients with PCOS.Methods:Computerized searches of the science,Medline,VIP,Wan Fang and China HowNet(CNKI)databases were conducted to identify eligible randomized controlled trials(RCTs)from the data obtained up to March 1,2022.The Cochrane Collaboration risk of bias tool was used to assess the risk of bias in individual RCTs,and R software(version 4.0.3)was used for data statistical analysis.Results:Nine RCTs involving 1035 patients were included.Comparing to D+M,significant reduce of LH(mean difference[MD]:-1.93,95%confidence interval[CI]:-3.44,-0.42;Unit:U/L P<0.01;I2=89%)、T(MD:-1.44,95%CI-2.59,-0.30;Unit:nmol/L P<0.01;I2=98%)and significant increase of E2(MD:31.43,95%CI 24.54,38.33;Unit:pmol/L P<0.01;I2=96%)were shown in TCM+D+M.Comparing to D+M,TCM+D+M group has higher ovulation rate(RR 1.1495%CI 1.07,1.22;P=0.42;I2=0%)and higher pregnancy rate(RR 1.2995%CI 1.15,1.44;P=0.37;I2=7%).There is no significant difference between the two therapies in FSH changes(MD:-1.00,95%CI-2.27,0.28;Unit:U/L P<0.01;I2=95%).Subgroup analysis showed that compared with the Guizhi Fuling capsule group,the Kuntai capsule group had more FSH reduction and E2 increase more.In other outcome indicators,the two subgroup did not show significant differences.Conclusion:Kuntai Capsule+Diane-35+Metformin is better than Guizhi Fuling Capsule in reducing FSH,and it is also better in increasing E2.There was no significant difference between the two in LH and T hormones.There was no significant difference between Kuntai Capsules+Diane-35+Metformin and Guizhi Fuling Capsules+Diane-35+Metformin.As for the effect in lessen insulin resistance,Kuntai Capsule+Diane-35+Metformin was significantly better than Guizhi Fuling Capsules+Diane-35+Metformin.
文摘The core sampling experiments were conducted after hydraulic fracturing in the three-dimensional development zone of Fuling shale gas.Six coring wells of different well types were systematically designed.Based on the integrated engineering technology of post-fracturing drilling,coring and monitoring of shale and the analysis of fracture source tracing,the evaluation of the fracture network after fracturing in the three-dimensional development of shale gas was conducted.The data of core fractures after fracturing indicate that three major types of fractures are formed after fracturing:natural fractures,hydraulic fractures,and fractures induced by external mechanical force,which are further classified into six subcategories:natural structural fractures,natural bedding fractures,hydraulic fractures,hydraulically activated fractures,drilling induced fractures,and fractures induced by core transportation.The forms of the artificial fracture network after fracturing are complex.Hydraulic fractures and hydraulically activated fractures interweave with each other,presenting eight forms of artificial fracture networks,among which the“一”-shaped fracture is the most common,accounting for approximately 70%of the total fractures.When the distance to the fractured wellbore is less than 35 m,the density of the artificial fracture network is relatively high;when it is 35–100 m,the density is lower;and when it is beyond 100 m,the density gradually increases.The results of the fracture tracing in the core sampling area confirm that the current fracturing technology can essentially achieve the differential transformation of the reservoir in the main area of Jiaoshiba block in Fuling.The three-layer three-dimensional development model can efficiently utilize shale gas reserves,although there is still room for improvement in the complexity and propagation uniformity of fractures.It is necessary to further optimize technologies such as close-cutting combined with temporary blocking and deflection within fractures or at fracture mouths,as well as limited flow perforation techniques,to promote the balanced initiation and extension of fractures.
基金financially supported by the Natural Science Foundation of China (NSFC Grant 41572106)+1 种基金the National Science and Technology Major Project "The enrichment conditions, evaluation technology and application of shale gas in the Sichuan Basin and its periphery" (Item No. 2017ZX05035002-006)State Key Laboratory of oil and gas resources and exploration, Chinese University of Petroleum-Beijing
文摘Comprehensive quantitative evaluation of shale gas content and the controlling factors in different occurrence states is of great significance for accurately assessing gas-bearing capacity and providing effective well-production strategies. A total of 122 core samples from well JY-A in the Fuling shale gas field were studied to reveal the characteristics of S_1 l shale,15 of which were selected to further predict the shale gas content in different occurrence states, which are dependent on geological factors in the thermal evolution process. Geological parameters were researched by a number of laboratory programs, and the factors influential in controlling shale gas content were extracted by both PCA and GRA methods and prediction models were confirmed by the BE method using SPSS software. Results reveal that the adsorbed gas content is mainly controlled by TOC, Ro, SSA, PD and pyrite content, and the free gas content is mainly controlled by S_2, quartz content, gas saturation and formation pressure for S_1 l in well JY-A. Three methods, including the on-site gas desorption method, the empirical formula method, and the multiple regression analysis method were used in combination to evaluate the shale gas capacity of well JY-A, all of which show that the overall shale gas content of well JY-A is in the range of 2.0–5.0 m^3/t and that the free gas ratio is about 50%, lower than that of well JY-1. Cause analysis further confirms the tectonics and preservation conditions of S_1 l in the geological processes, especially the influence of eastern boundary faults on well JY-A, as the fundamental reasons for the differences in shale gas enrichment in the Jiaoshiba area.
基金supported by the Specialized Research Fund for the Doctoral Program of Higher Education(No.20130096140001)the 111 Project(No.B16046)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘Guizhi Fuling capsule(GFC), a traditional Chinese medicine(TCM) with effects of promoting blood circulation and dissipating blood stasis, has been widely used in the clinic. Because of the complex matrix and various chemical structure types, quality control of GFC remains great challenge. In the present study, an ultra performance liquid chromatography hybrid triple-quadrupole mass spectrometry(UPLC-QQQ MS) method with ultrafast positive/negative ionization switching was developed for simultaneous determination of 18 bioactive components in GFC, including methyl gallate, ethyl gallate, oxypaeoniflorin, benzoic acid, albiflorin, paeonolide, paeoniflorin, 1, 2, 3, 4, 6-pentagalloylglucose, mudanpioside C, benzoyloxypaeoniflorin, benzoylpaeoniflorin, pachymic acid, amygdalin, cinnamaldehyde, paeonol, cinnamic acid, 4-hydroxybenzoic acid, and gallic acid. Separation was performed on an Agilent Zorbax Extend–C18 column(2.1 mm × 50 mm, 1.8 μm), using a gradient elution with acetonitrile and water containing 0.1% formic acid. Cholic acid was selected as the internal standard. This newly developed method was fully validated for linearity, precision, accuracy, and stability, and then applied to quality assessment of GFC. Finally, the batch-to-batch reproducibility of GFC samples was evaluated by the cosine ration and Euclidean distance method, which showed high quality consistency. The results demonstrated that the developed method provided a reasonable and powerful manner for quality control of GFC.
基金Supported by the Sinopec Science and Technology Project(P22183).
文摘In the Jiaoshiba block of the Fuling shale gas field,the employed reserves and recovery factor by primary well pattern are low,no obvious barrier is found in the development layer series,and layered development is difficult.Based on the understanding of the main factors controlling shale gas enrichment and high production,the theory and technology of shale gas three-dimensional development,such as fine description and modeling of shale gas reservoir,optimization of three-dimensional development strategy,highly efficient drilling with dense well pattern,precision fracturing and real-time control,are discussed.Three-dimensional development refers to the application of optimal and fast drilling and volume fracturing technologies,depending upon the sedimentary characteristics,reservoir characteristics and sweet spot distribution of shale gas,to form"artificial gas reservoir"in a multidimensional space,so as to maximize the employed reserves,recovery factor and yield rate of shale gas development.In the research on shale gas three-dimensional development,the geological+engineering sweet spot description is fundamental,the collaborative optimization of natural fractures and artificial fractures is critical,and the improvement of speed and efficiency in drilling and fracturing engineering is the guarantee.Through the implementation of three-dimensional development,the overall recovery factor in the Jiaoshiba block has increased from 12.6%to 23.3%,providing an important support for the continuous and stable production of the Fuling shale gas field.
文摘One of the important indicators of shale gas reservoir excavation is capacity evaluation,which directly affects whether large-scale shale gas reservoirs can be excavated.Capacity evaluation is the basis of system analysis and dynamic prediction.Therefore,it is particularly important to conduct capacity evaluation studies on shale gas horizontal wells.In order to accurately evaluate the horizontal well productivity of shale gas staged fracturing,this paper uses a new method to evaluate the productivity of Fuling shale gas.The new method is aimed at the dynamic difference of horizontal wells and effectively analyzes the massive data,which are factors affecting the productivity of shale gas horizontal wells.According to the pressure system,production dynamic characteristics,well trajectory position,fracturing transformation mode and penetration depth,32 wells were divided into four types.Then,based on the classification,the principal component analysis methods can be used to evaluate the horizontal well productivity of shale gas.The new method of capacity evaluation has improved the accuracy by 10.25%compared with the traditional method,which provides a theoretical basis for guiding the efficient development of the horizontal wells of Fuling shale gas.
基金supported by the National Natural Science Foundation of China(Grant No.41872124 and 91755211)and several Sinopec in-house projects.We thank Sinopec Petroleum Exploration and Production Research Institute,Sinopec Exploration Company,Sinopec Jianghan Oilfield,and Sinopec East China Petroleum Company for valuable data and information.
文摘The Fuling shale gas field in China is the largest shale gas field as well as the largest of its type discovered in any Lower Paleozoic formation.In this study,the geology and production of the upper and lower gas layers in the Fuling shale gas field are evaluated in terms of structure,shale quality,fault,initial production,and estimated ultimate recovery(EUR).The shale in the lower gas layer of the Jiaoshiba anticline is a high-quality reservoir,where the space is dominated by organic pores in kerogen,and the gas content is high.The shale gas wells reveal relatively high initial production and EUR.However,the shale in the upper gas layer of the Jiaoshiba anticline has reservoir space mainly composed of clay mineral pores and organic pores within bitumen,and the gas content is low.In terms of structure,primary gas migration may occur in the upper gas layer,resulting in free gas accumulation in the structural high,where the development effects are generally better than those in the structural low.The lower gas layer in the Pingqiao anticline,is the main interval for shale gas accumulation and development due to the high-quality shale.Under the influence of faults,the efficiency of exploration wells emplaced on top of the anticline is much lower a compared with those in the flanks.The residual synclines close to the Sichuan Basin,including the Baima and Baitao anticlines,are characterized by more recent uplifts,larger area,greater distance from the deep and large faults,and early fracture closure.Therefore,we recommend that the shale gas exploration and development should be carried out preferentially in areas close to the center of the residual synclines,featuring relatively high-pressure coefficient and moderate burial depth.
文摘Geological characteristics,geomechanical behavior and hydraulic fracture propagation mechanism in the Marcellus shale gas play are analyzed and compared with China’s Fuling shale play.Successful experiences in hydraulic fracturing and shale gas development in the Marcellus shale gas play are summarized,which might be applicable in other shale plays.The main factors contributing to the successful development of the Marcellus shale gas play include adoption of advanced drilling and completion technologies,increases of hydraulic fracturing stages,proppant concentration and fluid injection volume.The geological and geomechanical mechanisms related to those technologies are analyzed,particularly the in-situ stress impacts on hydraulic fracturing.The minimum horizontal stress controls where the fractures are initiated,and the maximum horizontal stress dominates the direction of the hydraulic fracture propagation.Hydraulic fracturing performed in the shale reservoir normally has no stress barriers in most cases because the shale has a high minimum horizontal stress,inducing hydraulic fractures propagating beyond the reservoir zone,resulting in inefficient stimulation.This is a common problem in shale plays,and its mechanism is studied in the paper.It is also found that the on-azimuth well has a higher productivity than the off-azimuth well,because shear fractures are created in the off-azimuth well,causing main fractures to kink and increasing fracture tortuosity and friction.The Fuling shale gas play has a markedly higher minimum horizontal stress and much smaller horizontal stress difference.The high minimum horizontal stress causes a much higher formation breakdown pressure;therefore,hydraulic fracturing in the Fuling shale gas play needs a higher treatment pressure,which implies higher difficulty in fracture propagation.The small difference in the two horizontal stresses in the Fuling shale gas play generates shorter and more complex hydraulic fractures,because hydraulic fractures in this case are prone to curve to preexisting fractures.To overcome these difficulties,we recommend reducing well spacing and increasing proppant concentration to increase gas productivity for the Fuling shale gas development.
基金Supported by National Key Research and Development Program of China:Study on the Core Efficacy Evaluation of Fuling(Poria)(No.2017YFC1703005)。
文摘OBJECTIVE:To observe and explore the effect of Fuling(Poria) in alleviating the spleen deficiency symptom pattern(SDSP).METHODS:We established an animal model of SDS in Sprague-Dawley(SD) rats by treating them with deficiency-inducing factors,including irregular feeding and tail clamping.Mice were administered Fuling(Poria) and its extracts(raw/cooked powder,aqueous/alcohol extract) by gavage once a day for 21 d.The body weight,rectal temperature,and spleen and thymus organ coefficients were calculated.The levels of motilin(MTL),gastrin(GAS),aquaporin 2(AQP2),interleukin 2(IL-2),IL-4,and 5-hydroxytryptamine(5-HT) in the serum and the level of AQP2 in the kidneys were evaluated by enzyme-linked immunosorbent assay.RESULTS:Fuling(Poria) and its extracts did not change the body weight,rectal temperature,and organ coefficients of the spleen and thymus.However,it reduced the levels of MTL and GAS and increased the levels of IL-2 and AQP2.In addition,the levels of IL-4 and 5-HT showed no significant alteration.CONCLUSIONS:These results suggested the crucial function of Fuling(Poria) in SDSP,especially promoting digestive function and water metabolism.
文摘Marine shale gas in South China is widely distributed and demonstrates an enriched resource. Compared with the North American commercial shale gas field, the shale formation in South China is featured by old age, multiple stages tectonic movements, and high thermal evolution degree and complex reservoir forming conditions. As a result, the existing theories and technical methods of exploration in North America cannot be simply applied to South China. Since 2007, based on the in-depth study on a large quantity of analytical test data, we have conducted the theory and technology research for the southern marine shale gas, found Wufeng-Longmaxi Group deep water shelf high-quality shale gas critical parameters coupling laws, proposed the “Binary Enrichment” theory for highly evolved marine shale gas in southern complex tectonic zone, and established a zone selection and evaluation criteria, which are based on the quality of shale, for the key of preservation condition, and for the purpose of economy. Taking this as a guide, we selected Longmaxi formation Lower Silurian of Fuling area in southeastern Sichuan Province as the preferred breakthrough field for the shale gas exploration. The Jiaoye 1 well has the daily gas production of 20.3 × 10<sup>4</sup> m<sup>3</sup>, which embarked the found of China’s first large-scale business development gas field, the Fuling shale gas field. And we submit the first domestic shale gas proven reserves of 1067.5 × 10<sup>8</sup> m<sup>3</sup>. The Fuling shale gas reservoirs are deep water shelf marine high-quality shale, with favorable thickness and even distribution, without dissection in the middle. They are typical self-generation and self-storage shale gas reservoirs. The gas fields have high production gas well;high pressure gas reservoir, good gas components and good exploit results, and there for the Fuling shale gas field is characterized by mid-depth, high pressure, high reservoir pressure, and high quality gas accumulation. Up to March 24, 2014, all of the 101 fracture-gas testing wells have obtained middle and upper shale airflow, with the average single well test production of 32.6 × 10<sup>4</sup> m<sup>3</sup>/d. In the exploration and development process, technology series in geological evaluation, horizontal well, drilling, well completion, piecewise fracturing techniques have been gradually formed. It is of great importance to commercially develop the domestic shale gas and promote the restructuring of China’s energy structure.
基金Project supported by National Science and Technology Major Project“Demonstration Project of Fuling Shale Gas Development”(No.:2016ZX05060).
文摘For the sake of figuring out the influential mechanisms of structural characteristics on the productivity of shale gas wells,the structural characteristics of the Jiaoshiba Block in the Fuling shale gasfield,Sichuan Basin,were analyzed.Then,based on well test data of more than 190 horizontal wells,the effects of structures on shale gas well productivity were discussed systematically,and the main structural factors of different structural units in the Jiaoshiba Block that influence the productivity of shale gas wells were clarified.The following results were obtained.First,the structural units in the Jiaoshiba Block were obviously different in structural characteristics and their deformation strength is different.Second,the influence of structural characteristics on shale gas well productivity is directly manifested in gas-bearing property and fracturing effect.The stronger the structural deformation and the more developed the large faults and natural fractures,the more easily shale gas escapes and the poorer the gas bearing property will be,and vice versa.Third,The stronger the structural deformation,the more developed the fractures,the greater the burial depth and the higher the compressive stress of negative structures,the worse the fracturing effect will be,and vice versa.And fourth,Tectonics is the key factor controlling the difference of shale gas productivity between different structural units in the Jiaoshiba Block,but the main structural factors influencing the productivity are different in different structural units.
基金the Korea Health R&D Project,No.A080863,Granted by Ministry of Health and Welfare,Republic of Korea
文摘Enhancing Schwann cell proliferation may be beneficial for peripheral nerve repair and nerve regeneration. A traditional herbal formula composed of Fuling （poria cocos）, Baizhu （Atractylodes macrocephala）, and Danggui （Angelica sinensis） （FBD） improves neuronal survival and growth, and FBD may promote the secretion of brain-derived neurotrophic factor. However, the mechanism underlying Schwann cell proliferation remains unclear. We tested whether FBD enhanced the proliferation of human Schwann cells. FBD （20 ug/mL） increased Schwann cell viability and survival and increased the number of cells at G2/M and S phases. FBD also increased nerve growth factor and brain-derived neurotrophic factor expression in Schwann cells, with maximum efficacy at 20 ug/mL.
基金supported by the Special and Significant Project of National Science and Technology“Enrichment law and exploration evaluation methods of shale gas in key areas”(No.2017ZX05036001)Sinopec Major Science and Technology Project“Target evaluation and key exploration techniques for large marine gas fields in Sichuan Basin”(No.P16082).
文摘Carbonate rocks in the first member of the Middle Permian Maokou Formation in the Sichuan Basin(hereinafter“Mao 1 Member”for short)have been taken as a set of carbonate source rocks,and they have not been specifically studied from the aspects of reservoir evaluation and testing.By referring the exploration ideas of unconventional natural gas,the Mao 1 Member in Fuling area of southeastern Sichuan Basin has obtained industrial gas flow in development wells in recent years.In order to further clarify the natural gas exploration potential of the Mao 1 Member in this area,it is necessary to study its sedimentary characteristics,natural gas accumulation conditions and main control factors based on the data of field section measurement,systematic coring in drilling and laboratory testing.The following research results were obtained.First,the gas reservoir in the Mao 1 Member in the Fuling area is of sourceereservoir integration,and its natural gas ismainly enriched in blackish graymarlite and nodularmarlite.Second,its reservoir spaces are dominated by grainmargin pores(fractures),diagenetic shrinkage pores(fractures),organic pores and fractures.Third,the pores aremostly in nanometer scale,and the main pore diameter is in the range of 5-50 nm,which is between shale reservoir and conventional reservoir,with strong heterogeneity.Fourth,the gas reservoir is characterized by source-reservoir coexistence,lithology controlling reservoir and extensive layered distribution,presenting two-stage differential hydrocarbon enrichment,namely intraformational near-source enrichment in the early stage and interformational blowdown adjustment in the late stage.Fifth,the development of blackish gray organic-rich fine marlite which is deposited with the episodic upwelling in the outer ramp facies belt is the foundation for the natural gas accumulation,the transformation of clay minerals controls the development of higher-quality reservoirs,good preservation conditions are the key to the natural gas accumulation,and fracture development is favorable for the enrichment and high yield of natural gas.In conclusion,theMao 1Member in this area is a special type of gas reservoir,i.e.,carbonate source rock gas reservoir,which has greater potential of natural gas exploration and industrial gas flow have been obtained in several wells.The discovery of such type of gas reservoirs not only expands the domain of natural gas exploration in the Sichuan Basin,but provides the reference for the natural gas exploration in other areas of South China.
基金supported by National Science and Technology Major Projects of China(2013ZX09402203,2013ZX09508104)Medical and Health Science and Technology Innovation Engineering of Chinese Academy of Medical Sciences(2016-I2M-3-007)National Natural Science Foundation of China(81573454)
文摘OBJECTIVE To evaluate the effect of Guizhi Fuling Capsule active pharmaceutical ingredient(API)and its fractions on human breast cancer cells proliferation by high-throughput screening assay.METHODS The crude fractions were obtained from the extraction and elution of the API of Guizhi Fuling Capsule,and 929 standard fractions were obtained by the optimal separation conditions.Sulforhodamine B(SRB)method was used to evaluate the effects of the Guizhi Fuling capsule API and929 kinds of fractions on the proliferation of human breast cancer cells MCF-7 and MDA-MB-231.RESULTS The Guizhi Fuling capsule API had a strong ability to inhibit the proliferation of MCF-7 cells at high concentration and the ability to inhibit MDA-MB-231 cells' proliferate at low concentration following 72 h treatment;some samples of 929 fractions(5μg·mL^(-1))was found to have a breast cancer cell growth inhibition rate above 50%,without toxicity on HUVECs proliferation.CONCLUSION The API of Guizhi Fuling capsule had significant cytotoxicity effects on these two human breast cancer cells,with significant concentration-and time-dependent manner.
基金National administration of TCM base special fund(No.JDZX2015277)Beijing municipal science and technology commission science and technology star project(No.Z191100001119025)
文摘Objective:The mechanism of Chinese herbal medicine"Bi xie and Tu fuling"on the treatment of gouty arthritis was explored through network pharmacological methods.Methods:First,the TCMSP Chinese medicine system pharmacology database and analysis platform were used to define the bioavailability(OB)30%and drug-likeness(DL)0.18.Database to mine genetic targets related to gouty arthritis disease.Finally,the two genes are intersected to construct a Chinese medicine regulatory network of Chinese medicine-components-target genes-disease.The genes in the network are used to construct a protein interaction network to find core genes for analysis of GO and KEGG.Results:There were a total of 84 active ingredients in Chinese medicine Poria and Poria cocos,and 17 effective ingredients and 180 genetic targets were obtained after screening.600 disease targets for gouty arthritis were identified,and 58 were common target genes for both.PPI network analysis revealed that IL1β,VEGFA,MAPK1,IL10,and PTGS2 may be drugs for treating gouty arthritis.Key targets.GO enrichment analysis identified 1,399 entries(P<0.05),of which biological processes mainly include biological stimulation,biological regulation,cell metabolism,etc;KEGG pathway enrichment analysis identified 152 signal pathways,of which signal pathways involved inflammation,metabolism,In terms of aging,mainly including the IL-4,IL-10,IL-13,IL-17signal pathway,etc.Conclusion:"Bi xie and Tu fuling"drugs have anti-inflammatory and immunological effects on gouty arthritis through multiple targets and multiple pathways.The mechanism of lowering uric acid and protecting liver and kidney is not clear.It needs molecular biology research to improve it.The mechanism of the action of the"Bi xie and Tu fuling"medicine pair provides new ideas for the clinical prescriptions.
基金National Natural Science Foundation of China(No.81473566)。
文摘Objective:To explore the main active components and mechanism of Guizhi Fuling pill in the treatment of ovarian cancer based on network pharmacology and molecular docking technology.Methods:TCMSP database was used to retrieve and screen the active components of each Chinese medicine in Guizhi Fuling pill.The main active components of the medicine were screened through the network analysis function of Cytoscape software,and the target of the main active components of the medicine was found in UniProt database.Using genecards,OMIM,digsee,TTD,and drugbank database to retrieve the related targets of ovarian cancer.The interaction between the target of the main active components of the drug and the related target of ovarian cancer was obtained by R software.The intersection targets are imported into string database for PPI analysis,and then the results are imported into Cytoscape to get the hub gene using the cyclohubba plug-in.KEGG pathway analysis and go enrichment analysis were carried out in metascape database.At last,autodock tools was used to verify the main active ingredients and the selected target proteins.PyMOL and LigPlus software visualized the results.Results:The main active components of Guizhi Fuling pills were quercetin,β-sitosterol,kaempferol,hederagenin,catechin,ellagic acid,stigmasterol and Douglas fir.There are 20 pivotal genes of drug effect on ovarian cancer,including VEGFA,AKT1,mapk8,Jun,MMP9,IL6,TNF,CXCL8,PTGS2,TP53,CASP3,mapk1,EGF,ESR1,EGFR,Myc,FOS,CCL2,CXCL8 and IL1B.According to KEGG pathway analysis,10 pathways with the highest correlation were found:cancer signaling pathway,MAPK signaling pathway,fluid shear stress and atherosclerosis,hepatitis B,trypanosomiasis,age-rage signaling pathway in diabetic complications,tumor necrosis factor signaling pathway,pertussis,IL-17 signaling pathway,and bladder cancer.Go enrichment analysis has screened out 10 important items in molecular biological function,cell components and biological process.The proteins encoded by IL6,mapk1,VEGFA,AKT1,TP53,TNF and CCL2 were used as targets to dock with the main active components of the drug respectively,and the best results were TNFαand ellagic acid.Conclusion:The main active components of Guizhi Fuling pill regulate multiple signal pathways by regulating IL6,VEGFA,CCL2,TNF,MMP9 and other cytokines,and inhibit the proliferation and metastasis of cancer cells.
基金This study was supported by National Science Foundation of China(81973645,81673805,81704058,and 81774100)Natural Science Foundation of Guangdong(2019A1515011560)Traditional Chinese Medicine Bureau of Guangdong Province(20161167,20201392).
文摘Objective:To investigate the mechanism of action of Guizhi Fuling pill in the treatment of atherosclerosis with network pharmacology.Methods:The active components of Guizhi Fuling pill were screened by specific ADME features on Traditional Chinese Medicine Systems Pharmacology platform,and their targets were obtained.Targets of atherosclerosis were extracted and screened from GeneCards database,OMIM database,DrugBank database and DisGeNET database,and the intersection of component targets and disease targets were regarded as potential targets.The protein-protein interaction network of potential targets was constructed by STRING database and Cytoscape 3.7.2.GO analysis and KEGG pathway analysis of potential targets were performed by Metascape platform.The“drugs-componenst-targets-pathways”network was constructed by Cytoscape 3.7.2.SwissDock was used for molecular docking of active components and key targets.Results:The main components of Guizhi Fuling pill in the treatment of atherosclerosis were quercetin,beta-sitosterol,kaempfero,hederagenin,baicalein,etc.The key targets mainly included PGST2,RELA,AKT1,etc.Molecular docking analysis showed that the binding energy of the main active components to the core target was less than-7 kcal/mol.The associated pathways mainly included fluid shear stress and atherosclerosis,PI3K-Akt signaling pathway and AGE-RAGE signaling pathway.Conclusion:Through this study,we initially revealed the possible mechanism of Guizhi Fuling pill in treating atherosclerosis through anti-inflammatory,reducing blood lipids,and regulating endothelial function.
文摘This paper reviews the research progress of Guizhi Fuling Pill in the clinical application of gynecology,such as the treatment of uterine leiomyoma,ovarian cyst,infertility and dysmenorrhea,in order to provide further research ideas for clinical researchers.
文摘Objective:To investigate the effect of Guizhi Fuling Capsule on sex hormones and tumor markers in patients with uterine fibroids, and observe its clinical efficacy.Methods: A total of 120 patients with uterine fibroids who were treated in our hospital from August 2016 to August 2017 were selected according to the order of treatment and divided into observation group and control group, 60 cases each. Mifepristone was administered to the control group and mifepristone combined with Guizhi Fuling Capsules was administered to the observation group. The levels of hemoglobin, vascular endothelial growth factor (VEGF), sex hormones, tumor markers, and inflammatory factors before and after treatment were compared between the two groups.Result:The levels of hemoglobin, vascular endothelial growth factor (VEGF), sex hormones, tumor markers, and inflammatory factors before and after treatment were compared between the two groups. After treatment, hemoglobin levels increased significantly in the two groups, VEGF, sex hormones FSH, LH and E2, tumor markers HE4, CA125, inflammatory cytokines TNF-α and IFN-γ levels decreased significantly. The hemoglobin level of the observation group was (117.09±11.51) g/L higher than that of the control group , and the level of VEGF was (0.69±0.06) pg/mL lower than that of the control group. The levels of sex hormone FSH, LH and E2 in the observation group were (15.87±1.56) U/L, (10.69±1.06) U/L and (4.95±0.51) pmol/L lower than those of the control group. The levels of HE4, CA125, TNF-α and IFN-γ gamma in the observation group were (52.47±5.24) pmol/L, (15.46±1.53) U/mL, (40.41±4.05) and (16.72±1.62) pg/mL lower than those of the control group.Conclusion:Guizhi Fuling Capsule can significantly improve the level of sex hormones and tumor markers, relieve inflammatory stress and improve the therapeutic effect.
基金This work was supported by the National Natural Science Foundation of China(No.82074348)the Taishan Scholar Project(tsqn201812145)the Key Technology Research and Development Program of Shandong(No.2019GSF108162).
文摘Objective To observe the effects of Guizhi Fuling capsule(GZFLC)on RPMI 8226 cells and explore the mechanisms.Methods Cell Counting Kit-8(CCK-8)assays and flow cytometry were used to detect the viability and apoptosis levels of RPMI 8226 cells.The effects on mitochondria were examined by ROS and JC-1 assays.Western blotting was used to detect the expression of B cell lymphoma-2(Bcl-2),Bax,cleaved caspase-3,and Apoptotic protease-activating factor 1(Apaf-1).Results GZFLC drug serum decreased the viability and increased the apoptosis of RPMI 8226 cells.In addition,this drug increased ROS levels and decreased the mitochondrial membrane potential(MMP).Western blotting showed that the Bcl-2/Bax ratios were decreased in the GZFLC drug serum-treated groups,whereas the expression levels of cleaved caspase-3 and Apaf-1 were increased.Conclusion GZFLC promoted apoptosis of myeloma cells through the mitochondrial apoptosis pathway.
