Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In ...Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In this study, four different fresh-cut types, whole flower(W),floret(F), quarterly cut floret(QF) and shredded floret(FS) of broccoli, were stored for 0, 1, 2 and 3 day(s) to explore GLS responses to postharvest treatments. As a result, seven GLS were identified, mainly including glucoraphanin(RAA), neoglucobrassicin(NEO), and glucobrassicin(GBC)and accounting for 52.69%, 20.12% and 14.99% of the total GLS(21.92 ± 0.48) μmol · g ^(-1 )DW, respectively. FS had the sharpest decrease in GLS after three days of storage(6.55 ± 0.37) μmol · g-1DW, while QF had the least(10.16 ± 0.33) μmol · g ^(-1 )DW. All GLS components decreased over storage, except for 4-methoxyglucobrassicin(4 ME) in FS and QF, suggesting its key role in serious wound defence. The results suggested certain postharvest approaches influenced the flavour and nutrition of broccoli.展开更多
The aim of this study was to investigate the effect of storage conditions on the sensory quality, colour and texture of fresh-cut cabbage during the addition of ascorbic acid, citric acid and calcium chloride. Ascorbi...The aim of this study was to investigate the effect of storage conditions on the sensory quality, colour and texture of fresh-cut cabbage during the addition of ascorbic acid, citric acid and calcium chloride. Ascorbic acid maintained the overall quality for 14 days at 0℃ and 7 days at 5℃;no difference, however, was observed regarding browning of cut surface compared to the control sample at both storage temperatures. Calcium chloride maintained the overall quality and cut surface browning for 14 days at both storage temperatures. It was also found that citric acid 1% can be used for minimally processed cabbage. Soaking with citric acid helped retain the color and increased the overall acceptance and organoleptic quality of fresh cut cabbage;it reduced browning of the cut surface and protected against formation of black specks. Citric acid treatment combined with low temperature storage (0℃) prolonged the shelf life of minimally processed cabbage for 22 days, time sufficient for acceptable marketing of the product. The lightness of minimally processed cabbage decreased linearly from 70.94 ± 6 to 63.8 ± 8.5 - 61.3 ± 8 units for the chemical treatments during 22 days of storage at 0℃. Hue angle values during storage time were also significantly influenced by chemical treatments mainly at 0℃.展开更多
At present, chlorine dioxide has been widely used as a bactericide that may become an alternative to chlorine. In this study, fresh-cut narrow-leaf cattail was soaked with 45, 70 and 95 mg/L chlorine dioxide solution,...At present, chlorine dioxide has been widely used as a bactericide that may become an alternative to chlorine. In this study, fresh-cut narrow-leaf cattail was soaked with 45, 70 and 95 mg/L chlorine dioxide solution, respectively. Fresh-cut narrow-leaf cattail samples were collected regularly to determine changes in surface microbe amount, cellulose content, Vc content, reduction sugar content and sensory quality of narrow-leaf cattail, thus analyzing the effects of chlorine diox- ide on sterilization and preservation of fresh-cut narrow-leaf cattail. The results showed that all three concentrations of chlorine dioxide solution could significantly reduce the amount of microbes on the surface of fresh-cut narrow-leaf cattail and improve the edible safety of products. The initial sterilization efficiency was im- proved gradually as the concentration of chlorine dioxide increased. In addition, chlorine dioxide treatment postponed the increase of cellulose content of fresh-cut narrow-leaf cattail. However, chlorine dioxide oxidized Vc and reducing sugar, and its bleaching effect also exerted a certain impact on the sensory quality of fresh- cut narrow-leaf cattail. Based on comprehensive comparisons, 45 - 70 mg/L chlorine dioxide solution exerted the best effects on sterilization and preservation of fresh-cut narrow-leaf cattail. This study laid the foundation for the production and application of chlorine dioxide solution and promotion of rapid development of nar- row-leaf cattail industry.展开更多
In this study, DERB1A transcription factor and stress-induced promoter rd29A were isolated respectively and amplified from Arabidopsis thaliana, se- quenced and analyzed by DNAsis. In addition, the stress-induced prom...In this study, DERB1A transcription factor and stress-induced promoter rd29A were isolated respectively and amplified from Arabidopsis thaliana, se- quenced and analyzed by DNAsis. In addition, the stress-induced promoter rd29A was utilized to construct the plant expression vector of DERB1A, which was transformed into Agrobacterium tumefaciens. Furthermore, the transgenic regeneration system of fresh-cut chrysanthemum from callus to plantlets was established successfully. On this basis, chrysanthemum leaf-disc explants were genetically transformed with Agrobacterium-mediated method. Two positive transgenie plantlets were obtained in vitro. Based on PCR detection, DREB1A transcription factor was integrated into chrysanthemum genome, which laid the foundation for breeding new transgenie cultivars of fresh-cut chrysanthemum with high comprehensive stress resistance, good cmalitv and high field.展开更多
[Objectives]This study was conducted to investigate the effect of compound fresh-keeping liquid on the browning and quality of fresh-cut yam during the cold chain process.[Methods]Under the simulated cold chain condit...[Objectives]This study was conducted to investigate the effect of compound fresh-keeping liquid on the browning and quality of fresh-cut yam during the cold chain process.[Methods]Under the simulated cold chain condition of 4℃,a uniform design(U6*(64))experiment was carried out to optimize the compound preservative formula,and a better compound preservative formula was obtained using the regression equation.[Results]The compound preservative formula was:AA 0.25%,CA 0.5%,Nisin 0.09%,GSH 0.15%.The compound fresh-keeping liquid effectively delayed the browning of fresh-cut yam and the accumulation of MDA,maintained the content of TSS,inhibited the activity of PAL and delayed the appearance of the maximum activity of POD and POD.[Conclusions]This study provides technical support for the actual extension of the shelf life of fresh-cut yam.展开更多
The present work focused on the effects of the Modified Atmosphere Packaging (MAP) 1 (5% O2 and 10% CO2) or 2 (2% O2 and 5% CO2) and the previous addition of Lactoperoxidase System (LPS) and Oregano essential oil or c...The present work focused on the effects of the Modified Atmosphere Packaging (MAP) 1 (5% O2 and 10% CO2) or 2 (2% O2 and 5% CO2) and the previous addition of Lactoperoxidase System (LPS) and Oregano essential oil or chlorine washing on the quality of fresh-cut lettuce during refrigerated storage at +4?C. Our results showed the significant effect of this combined treatment on quality improvement during storage. Thus, mesophilic bacteria was reduced in treated samples compared to those untreated with number which not exceeded the critical of 5 × 107 UFC?g-1 (p 2 and CO2 levels created by both atmosphere were not significantly different between the two treatments (p > 0.05). Brightness of lettuce samples was significantly reduced during storage. Thereafter, the PCA data showed the effect of combined treatment on the preservation of hygienic, physico-chemical and sensory quality up to the 7th day of refrigerated storage of these treated samples. The results obtained draw attention to modified atmosphere packaging lettuce and the addition of bio-preservatives which could be an alternative of choice to replace chlorine to preserve the sanitary quality of green products.展开更多
Fresh-cut cattail was extremely not resistant to store at room temperature.In the first day,it began to etiolate and rot,the fiber content increased,but the vitamin C content and reducing sugar content decreased.The a...Fresh-cut cattail was extremely not resistant to store at room temperature.In the first day,it began to etiolate and rot,the fiber content increased,but the vitamin C content and reducing sugar content decreased.The aerobic bacterial count increased,the weight loss rate sharply increased,and the sensory quality and food value were gradually lost.Low temperature storage could decrease the loss of vitamin C and reducing sugar of fresh-cut cattail,reduce the weight loss rate and delay the increase of fiber content,maintain the water and nutrient of fresh-cut cattail,and the storage effect of 0 ℃ was better than 4 ℃.展开更多
Winter squash (var. Ajijimang) washed in 100 mg.L-1 chlorine water and cut into 4 and 8 parts was packed in different packaging materials, 35 μm micro-perforated P-Plus (MPP), 50 μm polyethylene (PE), 80 μm nylon/P...Winter squash (var. Ajijimang) washed in 100 mg.L-1 chlorine water and cut into 4 and 8 parts was packed in different packaging materials, 35 μm micro-perforated P-Plus (MPP), 50 μm polyethylene (PE), 80 μm nylon/PE (Ny/PE), and 90 μm PE films separately. Fresh-cut samples were then stored at 10℃ for up to 17 days. The 35 μm MPP having a high oxygen transmission rate (OTR) has the most stable gas exchange ratio while the 80 μm Ny/PE was the most unstable. Firmness of the mesocarp remained acceptable until the end of storage regardless of the cutting size. Soluble solids content (SSC) remained stable and even slightly increased to a certain extent within 6 days. An increase in pH after 10-day storage suggested the activity of microorganisms within the packaging films. Strong off-odor was detected in samples packaged in 80 μm Ny/PE film after 13 days of storage. Mold was observed in all 1/4 cut samples on day 13 except the sample packaged with 80 μm Ny/PE film. However, no mold was found in all 1/8 cut samples except 35 μm MPP film on day 13. Severe mold invasion was apparent on the onset of the 17-day storage in 35 μm MPP film and 50 μm PE film. Samples cut into 8 parts and packed in 80 μm Ny/PE and 90 μm PE films had less mold incidence compared with other two packaging films. Fresh-cut winter squash packaged in 90 μm PE film maintained quality with shelf-life of 10 days at 10℃. Winter squash as fresh-cut commodity, with the right packaging material has the potential for longer period of storage in retail stores.展开更多
[Objectives]The paper was to study the effect of magnetic field technology on physicochemical properties of fresh-cut apple during storage.[Methods]Using fresh-cut apples as materials,magnetic field assisted refrigera...[Objectives]The paper was to study the effect of magnetic field technology on physicochemical properties of fresh-cut apple during storage.[Methods]Using fresh-cut apples as materials,magnetic field assisted refrigeration and conventional refrigeration control groups were set up,and the preservation effects of magnetic field assisted refrigeration on fresh-cut apples were tested by measuring the changes of microorganisms and physicochemical indicators.In this experiment,the conditions of magnetic field assisted refrigeration group was set as follows:temperature 4℃,magnetic field intensity 5 mT,static magnetic field,and storage for 6 d.[Results]At 6 d post storage,the weight loss rate,total number of colonies and total number of fungi of fresh-cut apples increased;the L value and hardness showed a downward trend;the soluble solid content first increased and then decreased;and the total acid decreased first and then increased.The above indicators in magnetic field assisted refrigeration group changed more gently than that in conventional refrigeration group.Compared with conventional assisted refrigeration,the weight loss rate of fresh-cut apples treated by magnetic field combined with 4℃refrigeration was reduced by 68.00%;the hardness was increased by 12.23%;the total number of colonies and total number of fungi were reduced by 64.15%and 37.50%;and the L value did not change much.High content of soluble solids and total acid was maintained.[Conclusions]Magnetic field assisted refrigeration delays quality deterioration of fresh-cut apples during storage,prolongs shelf life,and further improves their edible quality and commodity value.展开更多
Ethylene is one of the most important plant hormones that regulate physiological responses, maturity and senescence of postharvest fruits and vegetables. The determination of its role in the wound-healing response of ...Ethylene is one of the most important plant hormones that regulate physiological responses, maturity and senescence of postharvest fruits and vegetables. The determination of its role in the wound-healing response of fruits and vegetables is helpful in understanding the wound responses. 2,5-Norbornadiene （NBD）, inhibitor ethylene binding were used to determine the effect of ethylene action on wound-induced response of fresh-cut winter squash, a kind of vegetables known to produce ethylene markedly after cutting. The effect of NBD on the physiological and biochemical changes of fresh-cut winter squash was investigated in this experiment. The results showed that NBD had great effects on physio-biochemical metabolism of fresh-cut winter squash. Ethylene production was inhibited significantly by NBD. NBD also had an inhibition effect on polyphenol oxidase （PPO）, peroxidase （POD） and phenylalanine ammonia lyase （PAL） activity. The effect of NBD on lipoxygenase （LOX） activity and superoxide dismutase （SOD） was not obvious. NBD treated sample showed a more rapid increase in catalase （CAT） activity, which may help in healing the damage of the tissue. These information are important in understanding the fundamental biology of wound response and in the development of new technology to hasten wound-healing and, thereby, to minimize costly wound-related problems in fresh-cut industry.展开更多
The perceived visual quality of fruits and vegetables plays a central role in the choices made by retail customers.Machine learning(ML)approaches based on image analysis have been recently proposed to overcome the poo...The perceived visual quality of fruits and vegetables plays a central role in the choices made by retail customers.Machine learning(ML)approaches based on image analysis have been recently proposed to overcome the poor efficiency and subjectivity of human visual evaluation as well as the expensiveness and destructiveness of physical and chemical methods that measure internal indicators.In this paper,we propose a ML method based on Random Forests for estimating the chlorophyll and ammonia contents(considered,in the literature,reliable indicators of product freshness)from images of fresh-cut rocket leaves.Our approach copes with specific issues raised by(i)the non-uniform distributions of ammonia and chlorophyll values and(ii)the need to provide insights into the features that produce a particular model outcome,aiming to enhance its trustworthiness.Our experiments,performed on real images of fresh-cut rocket leaves,proved that the proposed approach significantly outperforms 7 competitor methods,obtaining an improvement of the RSE results of 6.6%for the prediction of the ammonia and of 10.4%for the prediction of the chlorophyll over its best competitor.Moreover,a specific analysis of the explainability of the predictions showed that the learned models are based on reasonable features,empowering their acceptance in real-world applications.展开更多
In present work,the effects of UV-B treatments on quality of fresh-cut spinach leaves were studied.For this purpose,spinach leaves grown in glasshouse were harvest,cleaned and placed into polystyrene foam dishes and w...In present work,the effects of UV-B treatments on quality of fresh-cut spinach leaves were studied.For this purpose,spinach leaves grown in glasshouse were harvest,cleaned and placed into polystyrene foam dishes and wrapped by stretch film.After packaging,freshcut spinach leaves were treated with UV-B irradiation and were stored at 5℃ temperature for 12 days.Total soluble solids,visual quality,L^*a^*b^* values,and also hue angle values of samples were determined during storage.Total soluble solids content of samples in control group has been higher than those from samples in UV-B treatments,however,visual quality was lower than the other treatments.According to L*values of the samples,it is found that lower ultraviolet irratiation(UV-B)treatments caused yellowing of samples,and also same result was obtained by b^* and a^* values of fresh-cut spinach leaves.Similarly,hue angles values of samples treated with UV-B for 6min was higher compared with the other treatments.Consequently,yellowing of fresh-cut spinach leaves was decreased,and visual quality scores of samples was increased through ultraviolet irradiation treatments.展开更多
Fresh-cut Chinese water chestnuts(CWCs)are prone to quality deterioration during storage,which does not meet consumer demand.In this study,the effect of exogenous melatonin(5 mmol·L^(-1))on the quality and potent...Fresh-cut Chinese water chestnuts(CWCs)are prone to quality deterioration during storage,which does not meet consumer demand.In this study,the effect of exogenous melatonin(5 mmol·L^(-1))on the quality and potential mechanisms in fresh-cut CWC was investigated.The results showed that melatonin treatment alleviated the cut-surface discoloration of CWCs.Not only did this treatment significantly slow down the in-crease in browning degree and yellowness(b*)as well as the decrease in lightness(L*),but it also significantly delayed the loss of weight and total soluble solids.Further investigations indicated that melatonin-treated fresh-cut CWCs exhibited significantly lower total phenolics and soluble quinones and suppressed the activities of phenylalanine ammonia-lyase,polyphenol oxidase,and peroxidase.Meanwhile,when fresh-cut CWCs were treated with melatonin,the total flavonoid concentration was significantly decreased compared to the control.Ad ditionally,melatonin significantly inhibited the accumulation of H_(2)O_(2)and malondialdehyde as well as enhanced the activities of superoxide dismutase and catalase by promoting the production of O_(2)^(-·).In summary,melatonin treatment may delay the surface discoloration of fresh-cut CWCs by inhibiting phenolic compound metabolism and improving antioxidant capacity,thereby effectively maintaining the quality and prolonging the shelf life of fresh-cut CWCs.展开更多
Designing environmentally friendly edible nanocomposite films with antioxidant functions is of great interest in the field of fresh-cut fruits.Herein,Pleurotus eryngii carbon quantum dots(PER-CDs)were prepared from Pl...Designing environmentally friendly edible nanocomposite films with antioxidant functions is of great interest in the field of fresh-cut fruits.Herein,Pleurotus eryngii carbon quantum dots(PER-CDs)were prepared from Pleu-rotus eryngii by-products,successfully characterized and added to chitosan(CS)/hydrogen-rich water solution to develop CDs/CS nanocomposite films(CS/PER-CDs),which was successfully applied to the preservation of fresh-cut pears.Thereinto,the fresh-cut pears treated with CS/2%PER-CDs nanocomposite films had minimum weight loss of 3.38%and the best retention of moisture,color,soluble solids and titratable acids after 5 days of storage.Importantly,the fresh-cut pears in the CS/2%PER-CDs treatment group had the lowest relative conductivity(37.53%)and MDA content of 1.94μmol/kg,which could indicate that the CS/2%PER-CDs nanocomposite films maintained good cell membrane integrity of fresh-cut pears.In addition,the CS/2%PER-CDs treatment group effectively reduced the maximum respiration intensity(51.67 mgCO_(2)/Kg⋅h)and ethylene production rate(0.75μg/kg⋅h)of fresh-cut pear.Therefore,PER-CDs can be added into chitosan-based coating film as a functional component to make nanocomposite films and applied in fresh-cut fruits preservation effectively.展开更多
Objectives:The purpose of this study was to evaluate the antibrowning functions of hawthorn leaf extract on fresh-cut potato and its possible mechanism.Materials and Methods:Fresh-cut potatoes were treated with differ...Objectives:The purpose of this study was to evaluate the antibrowning functions of hawthorn leaf extract on fresh-cut potato and its possible mechanism.Materials and Methods:Fresh-cut potatoes were treated with different concentrations(0.01%,0.05%,and 0.1%)of hawthorn leaf extract and preserved at 4 C for 8 days.The appears nee and colour of potato slices were evaluated,along with the content of the phenol,malondialdehyde(MDA),and hydrogen peroxide(H2O2)during cold storage.Meanwhile,the activities of polyphenol oxidase(PPO),peroxidase(POD),phenylalanine ammonia-lyase(PAL),lipoxygenase(LOX),catalase(CAT),superoxide dismutase(SOD),and the antioxidant capacity were determ in ed.Furthermore,the composition of hawthorn leaf extract was analyzed by high-performance liquid chromatographytandem mass spectrometry(HPLC-MS/MS).Results:The addition of hawthorn leaf extract effectively delayed the browning process.It not only enhanced the CAT activity and antioxidant capacity but also reduced the LOX activity and accumulation of MDA and H_(2)O_(2).Meanwhile,the activities of PPO,POD,and PAL as well as the content of phe nol were con trolled.Additionally,25 phe nols,34 flavonoids,and 5 proa nthocya nidins were identified through high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),ideluding caffeic acid,quercetin and catechol.Conelusion:Hawthorn leaf extract significantly alleviated the browning of fresh-cut potato.It could serve as a n atural an tibrowning alter native by stabilizing the membra ne and modulating reactive oxygen species and redox reactions.展开更多
The cut or sliced form of apples are more prone to chemical deterioration and microbial growth.In this work,quality changes(physicochemical properties,sensory quality,and microbial growth)of fresh-cut apples stored a...The cut or sliced form of apples are more prone to chemical deterioration and microbial growth.In this work,quality changes(physicochemical properties,sensory quality,and microbial growth)of fresh-cut apples stored at 4℃ for 15 days were studied after immersion treatment(water,biopolymer complex;free carvacrol,and carvacrol emulsion).Whey protein isolate/high methoxyl pectin-based carvacrol emulsions effectively delayed the browning process and reduced variation in chemical quality compared to other groups.Apples treated with carvacrol emulsions were more favored by panelists at the middle-and late-stages of storage.Additionally,the presence of carvacrol emulsions improved microbial safety,as demonstrated by the number of viable bacteria(<6 log CFU/g)and yeast and mold(<5 log CFU/g)after 15 d of storage.16 S rRNA Gene Sequencing was used to identify the bacterial species responsible for the spoilage of fresh-cut apples.These results show that carvacrol emulsions are a potential preservation alternative for fresh-cut apples.展开更多
基金supported by the Collaborative Innovation Center of the Beijing Academy of Agriculture and Forestry Sciences(Grant No.KJCX201915)the Youth Scientific Research Funds of Beijing Academy of Agriculture and Forestry Sciences(Grant No.QNJJ201914)the Innovation and Capacity-building Project of Beijing Academy of Agriculture and Forestry Sciences(Grant No.KJCX20200213).
文摘Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In this study, four different fresh-cut types, whole flower(W),floret(F), quarterly cut floret(QF) and shredded floret(FS) of broccoli, were stored for 0, 1, 2 and 3 day(s) to explore GLS responses to postharvest treatments. As a result, seven GLS were identified, mainly including glucoraphanin(RAA), neoglucobrassicin(NEO), and glucobrassicin(GBC)and accounting for 52.69%, 20.12% and 14.99% of the total GLS(21.92 ± 0.48) μmol · g ^(-1 )DW, respectively. FS had the sharpest decrease in GLS after three days of storage(6.55 ± 0.37) μmol · g-1DW, while QF had the least(10.16 ± 0.33) μmol · g ^(-1 )DW. All GLS components decreased over storage, except for 4-methoxyglucobrassicin(4 ME) in FS and QF, suggesting its key role in serious wound defence. The results suggested certain postharvest approaches influenced the flavour and nutrition of broccoli.
文摘The aim of this study was to investigate the effect of storage conditions on the sensory quality, colour and texture of fresh-cut cabbage during the addition of ascorbic acid, citric acid and calcium chloride. Ascorbic acid maintained the overall quality for 14 days at 0℃ and 7 days at 5℃;no difference, however, was observed regarding browning of cut surface compared to the control sample at both storage temperatures. Calcium chloride maintained the overall quality and cut surface browning for 14 days at both storage temperatures. It was also found that citric acid 1% can be used for minimally processed cabbage. Soaking with citric acid helped retain the color and increased the overall acceptance and organoleptic quality of fresh cut cabbage;it reduced browning of the cut surface and protected against formation of black specks. Citric acid treatment combined with low temperature storage (0℃) prolonged the shelf life of minimally processed cabbage for 22 days, time sufficient for acceptable marketing of the product. The lightness of minimally processed cabbage decreased linearly from 70.94 ± 6 to 63.8 ± 8.5 - 61.3 ± 8 units for the chemical treatments during 22 days of storage at 0℃. Hue angle values during storage time were also significantly influenced by chemical treatments mainly at 0℃.
基金Supported by Practice and Innovation Training Project for College Students in Jiangsu Province(2012JSSPITP3590)
文摘At present, chlorine dioxide has been widely used as a bactericide that may become an alternative to chlorine. In this study, fresh-cut narrow-leaf cattail was soaked with 45, 70 and 95 mg/L chlorine dioxide solution, respectively. Fresh-cut narrow-leaf cattail samples were collected regularly to determine changes in surface microbe amount, cellulose content, Vc content, reduction sugar content and sensory quality of narrow-leaf cattail, thus analyzing the effects of chlorine diox- ide on sterilization and preservation of fresh-cut narrow-leaf cattail. The results showed that all three concentrations of chlorine dioxide solution could significantly reduce the amount of microbes on the surface of fresh-cut narrow-leaf cattail and improve the edible safety of products. The initial sterilization efficiency was im- proved gradually as the concentration of chlorine dioxide increased. In addition, chlorine dioxide treatment postponed the increase of cellulose content of fresh-cut narrow-leaf cattail. However, chlorine dioxide oxidized Vc and reducing sugar, and its bleaching effect also exerted a certain impact on the sensory quality of fresh- cut narrow-leaf cattail. Based on comprehensive comparisons, 45 - 70 mg/L chlorine dioxide solution exerted the best effects on sterilization and preservation of fresh-cut narrow-leaf cattail. This study laid the foundation for the production and application of chlorine dioxide solution and promotion of rapid development of nar- row-leaf cattail industry.
基金Supported by Natural Science Foundation of Yunnan Province(2007C213M)Provincial Key Discipline of Landscape Plant&Ornamental Horticulture of Yunnan Province+1 种基金Provincial Key Lab of Colleges and Universities in Landscape Plants and Ornamental Horticulture of Yunnan ProvinceLarge Apparatuses Sharing Platform of Southwest Forestry University
文摘In this study, DERB1A transcription factor and stress-induced promoter rd29A were isolated respectively and amplified from Arabidopsis thaliana, se- quenced and analyzed by DNAsis. In addition, the stress-induced promoter rd29A was utilized to construct the plant expression vector of DERB1A, which was transformed into Agrobacterium tumefaciens. Furthermore, the transgenic regeneration system of fresh-cut chrysanthemum from callus to plantlets was established successfully. On this basis, chrysanthemum leaf-disc explants were genetically transformed with Agrobacterium-mediated method. Two positive transgenie plantlets were obtained in vitro. Based on PCR detection, DREB1A transcription factor was integrated into chrysanthemum genome, which laid the foundation for breeding new transgenie cultivars of fresh-cut chrysanthemum with high comprehensive stress resistance, good cmalitv and high field.
基金Natural Science Foundation of Guangxi(2017GXNSFAA198082)Guangxi Aquatic Vegetable Fresh-keeping and Processing Engineering Research Center Project(GXSSSCBXYJGZX1912)。
文摘[Objectives]This study was conducted to investigate the effect of compound fresh-keeping liquid on the browning and quality of fresh-cut yam during the cold chain process.[Methods]Under the simulated cold chain condition of 4℃,a uniform design(U6*(64))experiment was carried out to optimize the compound preservative formula,and a better compound preservative formula was obtained using the regression equation.[Results]The compound preservative formula was:AA 0.25%,CA 0.5%,Nisin 0.09%,GSH 0.15%.The compound fresh-keeping liquid effectively delayed the browning of fresh-cut yam and the accumulation of MDA,maintained the content of TSS,inhibited the activity of PAL and delayed the appearance of the maximum activity of POD and POD.[Conclusions]This study provides technical support for the actual extension of the shelf life of fresh-cut yam.
文摘The present work focused on the effects of the Modified Atmosphere Packaging (MAP) 1 (5% O2 and 10% CO2) or 2 (2% O2 and 5% CO2) and the previous addition of Lactoperoxidase System (LPS) and Oregano essential oil or chlorine washing on the quality of fresh-cut lettuce during refrigerated storage at +4?C. Our results showed the significant effect of this combined treatment on quality improvement during storage. Thus, mesophilic bacteria was reduced in treated samples compared to those untreated with number which not exceeded the critical of 5 × 107 UFC?g-1 (p 2 and CO2 levels created by both atmosphere were not significantly different between the two treatments (p > 0.05). Brightness of lettuce samples was significantly reduced during storage. Thereafter, the PCA data showed the effect of combined treatment on the preservation of hygienic, physico-chemical and sensory quality up to the 7th day of refrigerated storage of these treated samples. The results obtained draw attention to modified atmosphere packaging lettuce and the addition of bio-preservatives which could be an alternative of choice to replace chlorine to preserve the sanitary quality of green products.
基金Supported by Students Practice Innovation Projects in Jiangsu Province(2012JSSPITP3590)
文摘Fresh-cut cattail was extremely not resistant to store at room temperature.In the first day,it began to etiolate and rot,the fiber content increased,but the vitamin C content and reducing sugar content decreased.The aerobic bacterial count increased,the weight loss rate sharply increased,and the sensory quality and food value were gradually lost.Low temperature storage could decrease the loss of vitamin C and reducing sugar of fresh-cut cattail,reduce the weight loss rate and delay the increase of fiber content,maintain the water and nutrient of fresh-cut cattail,and the storage effect of 0 ℃ was better than 4 ℃.
文摘Winter squash (var. Ajijimang) washed in 100 mg.L-1 chlorine water and cut into 4 and 8 parts was packed in different packaging materials, 35 μm micro-perforated P-Plus (MPP), 50 μm polyethylene (PE), 80 μm nylon/PE (Ny/PE), and 90 μm PE films separately. Fresh-cut samples were then stored at 10℃ for up to 17 days. The 35 μm MPP having a high oxygen transmission rate (OTR) has the most stable gas exchange ratio while the 80 μm Ny/PE was the most unstable. Firmness of the mesocarp remained acceptable until the end of storage regardless of the cutting size. Soluble solids content (SSC) remained stable and even slightly increased to a certain extent within 6 days. An increase in pH after 10-day storage suggested the activity of microorganisms within the packaging films. Strong off-odor was detected in samples packaged in 80 μm Ny/PE film after 13 days of storage. Mold was observed in all 1/4 cut samples on day 13 except the sample packaged with 80 μm Ny/PE film. However, no mold was found in all 1/8 cut samples except 35 μm MPP film on day 13. Severe mold invasion was apparent on the onset of the 17-day storage in 35 μm MPP film and 50 μm PE film. Samples cut into 8 parts and packed in 80 μm Ny/PE and 90 μm PE films had less mold incidence compared with other two packaging films. Fresh-cut winter squash packaged in 90 μm PE film maintained quality with shelf-life of 10 days at 10℃. Winter squash as fresh-cut commodity, with the right packaging material has the potential for longer period of storage in retail stores.
基金Supported by Rural Revitalization Science and Technology Innovation Boost Action Plan of Shandong Province“Apple Step Processing and High Value Utilization Technology Research and Product Creation”(2022TZXD007-04).
文摘[Objectives]The paper was to study the effect of magnetic field technology on physicochemical properties of fresh-cut apple during storage.[Methods]Using fresh-cut apples as materials,magnetic field assisted refrigeration and conventional refrigeration control groups were set up,and the preservation effects of magnetic field assisted refrigeration on fresh-cut apples were tested by measuring the changes of microorganisms and physicochemical indicators.In this experiment,the conditions of magnetic field assisted refrigeration group was set as follows:temperature 4℃,magnetic field intensity 5 mT,static magnetic field,and storage for 6 d.[Results]At 6 d post storage,the weight loss rate,total number of colonies and total number of fungi of fresh-cut apples increased;the L value and hardness showed a downward trend;the soluble solid content first increased and then decreased;and the total acid decreased first and then increased.The above indicators in magnetic field assisted refrigeration group changed more gently than that in conventional refrigeration group.Compared with conventional assisted refrigeration,the weight loss rate of fresh-cut apples treated by magnetic field combined with 4℃refrigeration was reduced by 68.00%;the hardness was increased by 12.23%;the total number of colonies and total number of fungi were reduced by 64.15%and 37.50%;and the L value did not change much.High content of soluble solids and total acid was maintained.[Conclusions]Magnetic field assisted refrigeration delays quality deterioration of fresh-cut apples during storage,prolongs shelf life,and further improves their edible quality and commodity value.
文摘Ethylene is one of the most important plant hormones that regulate physiological responses, maturity and senescence of postharvest fruits and vegetables. The determination of its role in the wound-healing response of fruits and vegetables is helpful in understanding the wound responses. 2,5-Norbornadiene （NBD）, inhibitor ethylene binding were used to determine the effect of ethylene action on wound-induced response of fresh-cut winter squash, a kind of vegetables known to produce ethylene markedly after cutting. The effect of NBD on the physiological and biochemical changes of fresh-cut winter squash was investigated in this experiment. The results showed that NBD had great effects on physio-biochemical metabolism of fresh-cut winter squash. Ethylene production was inhibited significantly by NBD. NBD also had an inhibition effect on polyphenol oxidase （PPO）, peroxidase （POD） and phenylalanine ammonia lyase （PAL） activity. The effect of NBD on lipoxygenase （LOX） activity and superoxide dismutase （SOD） was not obvious. NBD treated sample showed a more rapid increase in catalase （CAT） activity, which may help in healing the damage of the tissue. These information are important in understanding the fundamental biology of wound response and in the development of new technology to hasten wound-healing and, thereby, to minimize costly wound-related problems in fresh-cut industry.
基金supported by the project FAIR-Future AI Research(PE00000013)spoke 6–Symbiotic AI,under the NRRP MUR program funded by the NextGenerationEU and by the project Prin 2017“SUS&LOW-Sustaining low-impact practices in horticulture through non-destructive approach to provide more information on fresh produce history and quality”(grant number:201785Z5H9)from the Italian Ministry of University and Research。
文摘The perceived visual quality of fruits and vegetables plays a central role in the choices made by retail customers.Machine learning(ML)approaches based on image analysis have been recently proposed to overcome the poor efficiency and subjectivity of human visual evaluation as well as the expensiveness and destructiveness of physical and chemical methods that measure internal indicators.In this paper,we propose a ML method based on Random Forests for estimating the chlorophyll and ammonia contents(considered,in the literature,reliable indicators of product freshness)from images of fresh-cut rocket leaves.Our approach copes with specific issues raised by(i)the non-uniform distributions of ammonia and chlorophyll values and(ii)the need to provide insights into the features that produce a particular model outcome,aiming to enhance its trustworthiness.Our experiments,performed on real images of fresh-cut rocket leaves,proved that the proposed approach significantly outperforms 7 competitor methods,obtaining an improvement of the RSE results of 6.6%for the prediction of the ammonia and of 10.4%for the prediction of the chlorophyll over its best competitor.Moreover,a specific analysis of the explainability of the predictions showed that the learned models are based on reasonable features,empowering their acceptance in real-world applications.
文摘In present work,the effects of UV-B treatments on quality of fresh-cut spinach leaves were studied.For this purpose,spinach leaves grown in glasshouse were harvest,cleaned and placed into polystyrene foam dishes and wrapped by stretch film.After packaging,freshcut spinach leaves were treated with UV-B irradiation and were stored at 5℃ temperature for 12 days.Total soluble solids,visual quality,L^*a^*b^* values,and also hue angle values of samples were determined during storage.Total soluble solids content of samples in control group has been higher than those from samples in UV-B treatments,however,visual quality was lower than the other treatments.According to L*values of the samples,it is found that lower ultraviolet irratiation(UV-B)treatments caused yellowing of samples,and also same result was obtained by b^* and a^* values of fresh-cut spinach leaves.Similarly,hue angles values of samples treated with UV-B for 6min was higher compared with the other treatments.Consequently,yellowing of fresh-cut spinach leaves was decreased,and visual quality scores of samples was increased through ultraviolet irradiation treatments.
基金the National Natural Science Foundation of China(No.32001764)the Student Scientific Research Program of Wuhan Polytechnic University(No.xsky2021002),China.
文摘Fresh-cut Chinese water chestnuts(CWCs)are prone to quality deterioration during storage,which does not meet consumer demand.In this study,the effect of exogenous melatonin(5 mmol·L^(-1))on the quality and potential mechanisms in fresh-cut CWC was investigated.The results showed that melatonin treatment alleviated the cut-surface discoloration of CWCs.Not only did this treatment significantly slow down the in-crease in browning degree and yellowness(b*)as well as the decrease in lightness(L*),but it also significantly delayed the loss of weight and total soluble solids.Further investigations indicated that melatonin-treated fresh-cut CWCs exhibited significantly lower total phenolics and soluble quinones and suppressed the activities of phenylalanine ammonia-lyase,polyphenol oxidase,and peroxidase.Meanwhile,when fresh-cut CWCs were treated with melatonin,the total flavonoid concentration was significantly decreased compared to the control.Ad ditionally,melatonin significantly inhibited the accumulation of H_(2)O_(2)and malondialdehyde as well as enhanced the activities of superoxide dismutase and catalase by promoting the production of O_(2)^(-·).In summary,melatonin treatment may delay the surface discoloration of fresh-cut CWCs by inhibiting phenolic compound metabolism and improving antioxidant capacity,thereby effectively maintaining the quality and prolonging the shelf life of fresh-cut CWCs.
基金financially supported by the National Key R&D Pro-gram of China(2018YFD0700303)the Shandong Province Technology Innovation Guidance Program(66812211118)+1 种基金the Shandong Province Modern Agricultural Industry Technology System(SDAIT-07-08)the Breeding Plan of Shandong Provincial Qingchuang Research Team(Innovation Team of Functional Plant Protein-Based Food),Natural Science Foundation of Shandong Province(ZR2022QC104).
文摘Designing environmentally friendly edible nanocomposite films with antioxidant functions is of great interest in the field of fresh-cut fruits.Herein,Pleurotus eryngii carbon quantum dots(PER-CDs)were prepared from Pleu-rotus eryngii by-products,successfully characterized and added to chitosan(CS)/hydrogen-rich water solution to develop CDs/CS nanocomposite films(CS/PER-CDs),which was successfully applied to the preservation of fresh-cut pears.Thereinto,the fresh-cut pears treated with CS/2%PER-CDs nanocomposite films had minimum weight loss of 3.38%and the best retention of moisture,color,soluble solids and titratable acids after 5 days of storage.Importantly,the fresh-cut pears in the CS/2%PER-CDs treatment group had the lowest relative conductivity(37.53%)and MDA content of 1.94μmol/kg,which could indicate that the CS/2%PER-CDs nanocomposite films maintained good cell membrane integrity of fresh-cut pears.In addition,the CS/2%PER-CDs treatment group effectively reduced the maximum respiration intensity(51.67 mgCO_(2)/Kg⋅h)and ethylene production rate(0.75μg/kg⋅h)of fresh-cut pear.Therefore,PER-CDs can be added into chitosan-based coating film as a functional component to make nanocomposite films and applied in fresh-cut fruits preservation effectively.
基金sustained by the National Natural Science Foundation of China(No.32001765)the Open Project Program of the State Key Laboratory of Food Nutrition and Safety,China(SKLFNS-KF-202016).
文摘Objectives:The purpose of this study was to evaluate the antibrowning functions of hawthorn leaf extract on fresh-cut potato and its possible mechanism.Materials and Methods:Fresh-cut potatoes were treated with different concentrations(0.01%,0.05%,and 0.1%)of hawthorn leaf extract and preserved at 4 C for 8 days.The appears nee and colour of potato slices were evaluated,along with the content of the phenol,malondialdehyde(MDA),and hydrogen peroxide(H2O2)during cold storage.Meanwhile,the activities of polyphenol oxidase(PPO),peroxidase(POD),phenylalanine ammonia-lyase(PAL),lipoxygenase(LOX),catalase(CAT),superoxide dismutase(SOD),and the antioxidant capacity were determ in ed.Furthermore,the composition of hawthorn leaf extract was analyzed by high-performance liquid chromatographytandem mass spectrometry(HPLC-MS/MS).Results:The addition of hawthorn leaf extract effectively delayed the browning process.It not only enhanced the CAT activity and antioxidant capacity but also reduced the LOX activity and accumulation of MDA and H_(2)O_(2).Meanwhile,the activities of PPO,POD,and PAL as well as the content of phe nol were con trolled.Additionally,25 phe nols,34 flavonoids,and 5 proa nthocya nidins were identified through high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),ideluding caffeic acid,quercetin and catechol.Conelusion:Hawthorn leaf extract significantly alleviated the browning of fresh-cut potato.It could serve as a n atural an tibrowning alter native by stabilizing the membra ne and modulating reactive oxygen species and redox reactions.
基金The research was supported by National Natural Science Foundation of China(32172301).
文摘The cut or sliced form of apples are more prone to chemical deterioration and microbial growth.In this work,quality changes(physicochemical properties,sensory quality,and microbial growth)of fresh-cut apples stored at 4℃ for 15 days were studied after immersion treatment(water,biopolymer complex;free carvacrol,and carvacrol emulsion).Whey protein isolate/high methoxyl pectin-based carvacrol emulsions effectively delayed the browning process and reduced variation in chemical quality compared to other groups.Apples treated with carvacrol emulsions were more favored by panelists at the middle-and late-stages of storage.Additionally,the presence of carvacrol emulsions improved microbial safety,as demonstrated by the number of viable bacteria(<6 log CFU/g)and yeast and mold(<5 log CFU/g)after 15 d of storage.16 S rRNA Gene Sequencing was used to identify the bacterial species responsible for the spoilage of fresh-cut apples.These results show that carvacrol emulsions are a potential preservation alternative for fresh-cut apples.
